A membrane strip incubation method for western blotting and an assay method comprising the same

Abstract

The application discloses a membrane strip incubation method for protein blotting and a detection method containing the same, and belongs to the technical field of immunoblotting. The membrane strip incubation method comprises the following steps: incubating a membrane strip by using a membrane strip incubation device, and placing the membrane strip into an incubation groove; adding buffer washing liquid and blocking liquid to the incubation groove, and blowing the liquid to flow to perform membrane blocking; adding a sample to be detected to the incubation groove, and blowing the liquid to flow to perform sample incubation; adding buffer washing liquid to the incubation groove, and blowing the liquid to flow to perform membrane washing; adding buffer washing liquid and enzyme combination to the incubation groove, and blowing the liquid to flow to perform enzyme combination; adding buffer washing liquid to the incubation groove, and blowing the liquid to flow to perform membrane washing; adding distilled water and color developing liquid to the incubation groove, shielding the membrane strip from light, and blowing the liquid to flow to perform light-proof color developing; adding distilled water to the incubation groove, and blowing the liquid to flow to perform color developing termination; and after each operation process is completed, liquid in the incubation groove is absorbed. The membrane strip incubation method ensures that the membrane strip is fully contacted with detection liquid and reacts.

Description

Technical Field

[0001] This invention relates to the field of immunoblotting technology, and more particularly to a membrane strip incubation method for protein blotting and a detection method containing the same. Background Technology

[0002] Immunoblotting, also known as protein blotting, involves transferring proteins onto a membrane and then detecting them using antibodies. Furthermore, immunoblotting offers advantages such as large analytical capacity, high sensitivity, and strong specificity, making it widely used for detecting protein characteristics, expression, and distribution.

[0003] However, in the process of incubating using existing membrane strip incubation methods and existing immunoblotting instruments, the incubation tray needs to be shaken by the shaking mechanism of the immunoblotting instrument to ensure sufficient contact and reaction between the membrane strip and the detection solution in the incubation tank. During the shaking process, because the shaker swings back and forth vertically and the angle of swing is difficult to control, the detection solution is prone to overflow from the incubation tank, wasting the detection solution and contaminating the detection environment. Moreover, as the usage time increases, the shaking accuracy of the shaking mechanism is difficult to guarantee, which can easily lead to insufficient or excessive shaking. Therefore, there is an urgent need for a membrane strip incubation method that can ensure sufficient contact and reaction between the membrane strip and the detection solution and prevent overflow of the detection solution. Summary of the Invention

[0004] The purpose of this invention is to overcome at least one deficiency of the prior art and provide a membrane strip incubation method for Western blotting that facilitates sufficient contact and reaction between the membrane strip and the detection solution while preventing spillage of the detection solution; in addition, it also provides a detection method for Western blotting.

[0005] The technical solution of this invention to solve the above-mentioned technical problems is as follows: A membrane strip incubation method for Western blotting, comprising:

[0006] A membrane strip incubation device is used to incubate the membrane strip. The incubation device includes a base and an incubation tray. The base is provided with an incubation tray placement part. The incubation tray is placed horizontally on the incubation tray placement part. The incubation tray is provided with a plurality of incubation tanks at intervals for incubating the membrane strip.

[0007] The membrane strips to be tested are placed into the incubation tank respectively;

[0008] Buffer washing solution and sealing solution are added to each of the incubation tanks to wet the membrane strips, and the liquid in the incubation tanks is blown to seal the membrane for a first time.

[0009] adding a sample to be detected into each of the plurality of incubation grooves, blowing the liquid in the incubation groove to flow to incubate the sample for a second time length, and then sucking dry the liquid in the incubation groove;

[0010] adding a buffer washing solution into each of the plurality of incubation grooves, blowing the liquid in the incubation groove to flow to wash the membrane for a third time length, and then sucking dry the buffer washing solution in the incubation groove;

[0011] adding a buffer washing solution and an enzyme combination into each of the plurality of incubation grooves, blowing the liquid in the incubation groove to flow to combine the enzyme for a fourth time length, and then sucking dry the liquid in the incubation groove;

[0012] adding a buffer washing solution into each of the plurality of incubation grooves, blowing the liquid in the incubation groove to flow to wash the membrane for a fifth time length, and then sucking dry the buffer washing solution in the incubation groove;

[0013] adding distilled water and a chromogenic solution into each of the plurality of incubation grooves, shielding light for the membrane strip and blowing the liquid in the incubation groove to flow to develop color in the light for a sixth time length, and then sucking dry the liquid in the incubation groove;

[0014] adding distilled water into each of the plurality of incubation grooves, blowing the liquid in the incubation groove to flow to terminate the development of color for a seventh time length, and then sucking dry the distilled water in the incubation groove;

[0015] The blowing the liquid in the incubation groove to flow is blowing the liquid in the incubation groove by the blowing disturbance device and making the liquid in the incubation groove flow relative to the membrane strip placed in the incubation groove.

[0016] The membrane strip incubation method has the advantages that the membrane strip in the incubation groove is fully contacted with the liquid and fully reacts, the incubation effect of the membrane strip is improved, the disturbance degree of the liquid in the incubation groove is flexibly adjusted by controlling the blowing speed and the air volume, the disturbance of the liquid is suitable, the liquid is prevented from overflowing from the incubation groove in the incubation process, the blowing speed and the air volume are flexibly controlled by selecting the suitable adjustment gear according to the disturbance degree of the liquid required in the incubation process, the disturbance degree of the liquid is controlled with high precision, and the liquid in the incubation groove is blown in multiple blowing modes to realize different disturbance modes.

[0017] In addition, on the basis of the above technical solutions, the application can also be improved and have the following additional technical features.

[0018] According to one embodiment of the present application, the membrane strip incubation method for protein blotting further comprises: covering the outer periphery of the base with a transparent light shield so that the incubation environment is isolated from the outside. In this embodiment, the transparent light shield is used to isolate the incubation environment from the outside, so that a relatively closed space is formed inside the transparent light shield, the influence of external airflow on the space inside the transparent light shield is reduced or avoided, the disturbance effect of the air outlet structure on the liquid in the incubation tank is improved, and the light shielding and color development of the membrane strip during incubation can be achieved.

[0019] According to one embodiment of the present application, the membrane strip incubation method for protein blotting further comprises: driving the transparent light shield to move up and down in the vertical direction by a lifting drive device one, the lifting drive device one being arranged outside the base, and the transparent light shield being mounted on the lifting drive device one. In this embodiment, the lifting drive device one is used to drive the transparent light shield to move up and down, which facilitates the downward movement of the transparent light shield to cover the outer periphery of the base and the upward movement of the transparent light shield to expose the base.

[0020] According to one embodiment of the present application, the membrane strip incubation method for protein blotting further comprises: driving the air outlet structure arranged on the air blowing disturbance device to move up and down in the vertical direction by the lifting drive device one, the air outlet structure being mounted inside the transparent light shield, the air blowing disturbance device further comprising a fan for inputting air, and the air outlet structure being connected to the fan through an air conveying pipeline. In this embodiment, the lifting drive device one is used to adjust the distance between the air outlet structure and the incubation disc, so that the disturbance degree of the air blowing on the liquid can be adjusted by adjusting the distance between the air outlet structure and the incubation disc, thereby facilitating the increase of the disturbance mode and the disturbance degree and further improving the disturbance effect of the air outlet structure on the liquid in the incubation tank.

[0021] According to one embodiment of the present application, the membrane strip incubation method for protein blotting further comprises: adjusting the temperature inside the transparent light shield by a temperature adjusting device according to the requirement of the membrane strip for the incubation temperature, so that the temperature inside the transparent light shield and the temperature of the liquid in the incubation tank meet the requirement of the membrane strip for the incubation temperature. In this embodiment, the temperature inside the transparent light shield is adjusted by the temperature adjusting device, which facilitates the satisfaction of the requirement of the membrane strip for the incubation temperature, and is conducive to improving the adaptability of the membrane strip incubation device and the membrane strip incubation method to different temperature environments and ensuring the normal performance and accuracy of the membrane strip incubation under different temperature environments.

[0022] According to one embodiment of the present application, the membrane strip incubation method for protein blotting further comprises: during the incubation of the membrane strip, after the liquid in the incubation tank is absorbed, the incubation tank is blown by the blowing disturbance device and the residual liquid in the incubation tank is blown dry. In this embodiment, the residual liquid in the incubation tank is blown dry by blowing the incubation tank, which is conducive to more thoroughly removing the residual liquid in the incubation tank, further improving the accuracy of the membrane strip incubation.

[0023] According to one embodiment of the present application, the membrane strip incubation method for protein blotting further comprises: the incubation tank is placed by the incubation tank placing rack, the upper side of the incubation tank placing rack is provided with the incubation tank placing part; the incubation tank placing rack is supported by a plurality of elastic support structures arranged at intervals, the lower end of the plurality of elastic support structures is connected to the base in the vertical direction, and the incubation tank placing rack is connected to the upper end of the plurality of elastic support structures; during the blowing of the incubation tank by the blowing disturbance device, the elastic support structures are elastically deformed under the blowing action of the blowing disturbance device, and the incubation tank placed on the incubation tank placing part shakes.

[0024] In this embodiment, under the blowing action of the blowing disturbance device, the wind blows on the incubation tank, the wind force acts on the incubation tank, the elastic support structures are elastically deformed, and the incubation tank placed on the incubation tank placing part can shake. The liquid in the incubation tank not only flows relative to the membrane strip placed in the incubation tank, but also has the disturbance caused by the shaking of the incubation tank, which is conducive to increasing the disturbance mode and the disturbance degree, and further improving the disturbance effect of the air outlet structure on the liquid in the incubation tank.

[0025] According to one embodiment of the present application, before the membrane strip to be detected is placed in the incubation tank, the membrane strip incubation method for protein blotting further comprises:

[0026] A plurality of membrane strips are placed on the membrane strip placing rack, the membrane strip placing rack is arranged on one side of the base or mounted on the base, the membrane strip placing rack is located above the incubation tank placing part, a plurality of incubation slots of the incubation tank placed one by one on the incubation tank placing part are detachably provided with a plurality of placing structures, and the placing structures are respectively provided with placing parts for detachably placing the membrane strips;

[0027] The height position of the membrane strip rest rack in the vertical direction is adjusted by a lifting driving device II, which is installed on the base, and the membrane strip rest rack is installed on the lifting driving device II and can move up and down in the vertical direction under the driving of the lifting driving device II, and the rest part and the membrane strip resting on the rest part can extend into the incubation tank under the driving of the lifting driving device II.

[0028] In the embodiment, the membrane strip is conveniently rested on the rest part, and the membrane strip can be limited by the rest part to avoid being turned up during incubation. Further, the membrane strip resting on the rest part is conveniently extended into the incubation tank and taken out of the incubation tank by driving the membrane strip rest rack to move up and down by the lifting driving device II, so that the membrane strip is conveniently disassembled and assembled.

[0029] In addition, the detection method for the Western blotting method provided in the embodiment comprises the membrane strip incubation method for the Western blotting method, and air is blown to the incubated membrane strip by the air blowing disturbance device to dry the membrane strip.

[0030] The detection method for the Western blotting method in the embodiment is advantageous in that the membrane strip in the incubation tank is fully contacted with the liquid and fully reacts, and the incubation effect of the membrane strip is improved. The disturbance of the liquid is conveniently adapted, and the liquid is prevented from overflowing out of the incubation tank during incubation. Further, the membrane strip is dried by blowing air to the incubated membrane strip by the air blowing disturbance device, so that the membrane strip is dried, and the dried membrane strip is conveniently taken out for the next detection operation. Further, the membrane strip is dried by blowing air to the membrane strip, which is advantageous in improving the uniformity of the drying of the membrane strip.

[0031] According to one embodiment of the present application, during the process of blowing air to the incubated membrane strip by the air blowing disturbance device, the detection method for the Western blotting method further comprises heating the air in the air conveying pipeline by starting an air heating device. The air blowing disturbance device comprises a fan and an air conveying pipeline. The fan is arranged on one side of the base or installed on the base and used for inputting air. The air conveying pipeline is connected to the air outlet end of the fan. The air outlet structure is connected to the air outlet end of the air conveying pipeline and arranged above the incubation disc placing part. The air heating device is installed in the air conveying pipeline or arranged on the outer periphery of the air conveying pipeline.

[0032] In the embodiment, the air in the air conveying pipeline is heated by turning on the air heating device, so that the air in the air conveying pipeline is heated by the air heating device, the film strip is dried after the film strip incubation is completed, the efficiency and uniformity of drying are improved, and the dried film strip is convenient to take out for next detection operation. BRIEF DESCRIPTION OF DRAWINGS

[0033] In order to more clearly illustrate the technical solutions in the present application, the drawings needed in the embodiment description will be briefly introduced. Obviously, the drawings in the following description are only some embodiments of the present application, and other drawings can be obtained by those skilled in the art without creative effort.

[0034] Figure 1 Flow chart of the film strip incubation method for Western blotting method in the embodiment of the present application;

[0035] Figure 2 Structure diagram of the film strip incubation device in the embodiment of the present application;

[0036] Figure 3 Right view of the film strip incubation device in Figure 2

[0037] Figure 4 Right view of the film strip incubation device in Figure 3

[0038] Figure 5 Structure diagram of the film strip incubation device in Figure 2

[0039] Figure 6 Structure diagram of the transverse movement driving mechanism in the embodiment of the present application;

[0040] Figure 7 Structure diagram of the air outlet structure in the embodiment of the present application;

[0041] Figure 8 Structure diagram of the film strip placed on the film strip shelf in the embodiment of the present application;

[0042] Figure 9 Structure diagram of the incubation disc placed on the incubation disc shelf in the embodiment of the present application;

[0043] Figure 10 Structure diagram of the film strip placed on the shelf structure in the embodiment of the present application;

[0044] Figure 11 ​​​A structural schematic diagram of an elastic support structure according to an embodiment of the present application. DETAILED DESCRIPTION

[0045] In order to make the purposes, technical solutions and advantages of the present application clearer, the following further describes the embodiments of the present application in detail with reference to the accompanying drawings.

[0046] In order to make the purposes, technical solutions and advantages of the present application clearer, the following further describes the embodiments of the present application in detail with reference to the accompanying drawings.

[0047] In the following description, a large number of specific details are set forth in order to provide a thorough understanding of the present application, however, the present application can also be implemented in other manners different from those described herein, therefore, the protection scope of the present application is not limited by the specific embodiments disclosed below.

[0048] The embodiment provides a membrane strip incubation method for a protein blotting method, as shown in Figure 1 The method comprises the following steps.

[0049] The membrane strip 47 is incubated by using a membrane strip incubation device as shown in Figures 2 to 5 The incubation device comprises a base 2 and an incubation disc 33, the base 2 is provided with an incubation disc placing part, the incubation disc 33 is horizontally placed on the incubation disc placing part, and a plurality of incubation grooves 331 for incubating the membrane strip 47 are arranged on the incubation disc 33 at intervals.

[0050] The membrane strip 47 to be detected is placed into the incubation grooves 331 respectively.

[0051] Buffer washing liquid and blocking liquid are added into the plurality of incubation grooves 331 respectively, the membrane strip 47 is wetted, and the liquid in the incubation grooves 331 is blown to flow to perform membrane blocking for a first time length.

[0052] The sample to be detected is added into the plurality of incubation grooves 331 respectively, the liquid in the incubation grooves 331 is blown to flow to perform sample incubation for a second time length, and then the liquid in the incubation grooves 331 is sucked dry.

[0053] The buffer washing liquid is added into the plurality of incubation grooves 331 respectively, the liquid in the incubation grooves 331 is blown to flow to perform membrane washing for a third time length, and then the buffer washing liquid in the incubation grooves 331 is sucked dry.

[0054] The buffer washing liquid and enzyme conjugate are added into the plurality of incubation grooves 331 respectively, the liquid in the incubation grooves 331 is blown to flow to perform enzyme conjugation for a fourth time length, and then the liquid in the incubation grooves 331 is sucked dry.

[0055] The buffer washing solution is added into the plurality of incubation grooves 331 respectively, the liquid in the incubation grooves 331 is blown to flow to wash the membrane for a fifth time length, and then the buffer washing solution in the incubation grooves 331 is sucked dry;

[0056] The distilled water and the chromogenic solution are added into the plurality of incubation grooves 331 respectively, the membrane strip 47 is shaded, and the liquid in the incubation grooves 331 is blown to flow to develop color for a sixth time length, and then the liquid in the incubation grooves 331 is sucked dry;

[0057] The distilled water is added into the plurality of incubation grooves 331 respectively, the liquid in the incubation grooves 331 is blown to flow to terminate color development for a seventh time length, and then the distilled water in the incubation grooves 331 is sucked dry;

[0058] The liquid in the incubation grooves 331 is blown to flow by the blowing disturbance device blowing to the incubation grooves 331 and making the liquid in the incubation grooves 331 flow relative to the membrane strip 47 placed in the incubation grooves 331.

[0059] In the embodiment, the liquid in the incubation grooves 331 is blown to flow by the blowing disturbance device blowing to the incubation grooves 331 and making the liquid in the incubation grooves 331 flow relative to the membrane strip 47 placed in the incubation grooves 331, which is conducive to the membrane strip 47 in the incubation grooves 331 fully contacting and fully reacting with the liquid, and improves the incubation effect of the membrane strip 47; in addition, the disturbance degree of the liquid in the incubation grooves 331 can be flexibly adjusted by controlling the blowing speed and the air volume, so as to make the disturbance of the liquid suitable, avoid the liquid overflowing from the incubation grooves 331 in the incubation process, avoid wasting the liquid, and also avoid the liquid overflowing to pollute the detection environment; further, it is conducive to setting the blowing disturbance device with multiple gears for adjusting the speed and the air volume, so as to select a suitable adjustment gear according to the need of the disturbance degree of the liquid in the incubation process, and then flexibly control the blowing speed and the air volume, and the disturbance degree control precision of the liquid is high; in addition, a plurality of blowing modes can be used to blow the liquid in the incubation grooves 331 to realize different disturbance modes, and further improve the disturbance effect of the liquid.

[0060] It should be noted that in the process of adding the to-be-detected sample into the plurality of incubation grooves 331 in the embodiment, the number of the to-be-detected sample corresponding to each membrane strip 47 is recorded; the liquid added into the incubation groove 331 in the embodiment is the detection liquid; the first duration of the membrane strip incubation method in the embodiment is 5 minutes, the second duration is 30 minutes, the third duration is 5 minutes, the fourth duration is 30 minutes, the fifth duration is 5 minutes, the sixth duration is 15 minutes, and the seventh duration is 1 minute; the durations of the above-mentioned procedures can also be adjusted according to the needs, and the incubation procedures for incubating the membrane strip in the prior art in the field can also be referred to; further, in order to wash the membrane more fully, the procedure for washing the membrane in the embodiment is repeated 2 to 3 times; further, in order to develop the color more fully, the procedure for developing the color in the dark is repeated 1 to 2 times; in addition, other necessary operations and incubation environment during the incubation of the membrane strip can be referred to the incubation process for incubating the membrane strip in the prior art in the field, and will not be described here.

[0061] In the embodiment, as shown in Figures 2 to 5 , the membrane strip incubation device adopted includes a base 2, an incubation disc 33, a liquid adding device, a liquid sucking device, and a blowing disturbance device, the base 2 is provided with an incubation disc placing part; the incubation disc 33 is placed on the incubation disc placing part, and a plurality of incubation grooves 331 are provided on the incubation disc 33 at intervals; the liquid adding device is installed on the base 2; the liquid sucking device is installed on the base 2; the blowing disturbance device is installed on the base 2, and the blowing disturbance device is provided with an air outlet structure 9, the air outlet structure 9 is arranged above the incubation disc placing part, and the air outlet of the air outlet structure 9 faces the incubation grooves 331 of the incubation disc 33 horizontally placed on the incubation disc placing part.

[0062] In the embodiment, as shown in Figure 3 , Figure 5 and Figure 6As shown, the liquid adding device comprises a transverse movement driving mechanism 5, a transversely movable liquid adding seat, a plurality of detection liquid storage bottles 26, a plurality of delivery pumps, a plurality of detection liquid output pipes and a plurality of detection liquid input pipes. The transverse movement driving mechanism 5 is installed on the base 2. The transversely movable liquid adding seat is installed on the transverse movement driving mechanism 5 and can move transversely above the incubation tray 33 under the driving of the transverse movement driving mechanism 5. The plurality of detection liquid storage bottles 26 are provided. The base 2 is provided with bottle placing portions for placing the detection liquid storage bottles 26. The plurality of detection liquid storage bottles 26 are placed on the bottle placing portions. The plurality of detection liquid storage bottles 26 are respectively used for storing detection liquids. The detection liquids include buffer washing liquid, sealing liquid, enzyme combination, distilled water and color developing liquid. The plurality of delivery pumps are provided one-to-one corresponding to the plurality of detection liquid storage bottles 26. The plurality of delivery pumps are installed on the base 2 at intervals. The plurality of detection liquid output pipes are provided one-to-one corresponding to the plurality of delivery pumps. The input ends of the plurality of detection liquid output pipes are respectively connected to the delivery pumps. The output ends of the plurality of detection liquid output pipes are respectively connected to the transversely movable liquid adding seat. The plurality of detection liquid input pipes are provided one-to-one corresponding to the plurality of delivery pumps. The input ends of the plurality of detection liquid input pipes are respectively connected to the detection liquid storage bottles 26. The output ends of the plurality of detection liquid input pipes are respectively connected to the delivery pumps.

[0063] In this embodiment, as shown in Figure 3 , Figure 5 and Figure 6 , under the driving of the transverse movement driving mechanism 5, the transversely movable liquid adding seat moves transversely above the incubation tray 33 relative to the incubation tray 33, thereby driving the detection liquid output pipes to move transversely above the incubation tray 33 relative to the incubation tray 33. By starting the delivery pumps, the detection liquids can be sequentially input into the incubation grooves 331.

[0064] In this embodiment, as shown in Figure 3 , Figure 5 and Figure 6 , the lower part of the base 2 is provided with a support table 20. The support table 20 is provided with bottle placing grooves 201 which are open on the left side and serve as bottle placing portions, so as to facilitate the placement of the detection liquid storage bottles 26 in the bottle placing grooves 201.

[0065] In this embodiment, as shown in Figure 3 , Figure 5 and Figure 6 , the base 2 is provided with a support table 21. The support table 21 is installed with an incubation tray placing rack 3. The incubation tray placing rack 3 is provided with an incubation tray placing portion. In addition, the base 2 is also provided with a support table 25. The transverse movement driving mechanism 5 is installed on the support table 25.

[0066] In this embodiment, as shown in Figure 3 , Figure 5 and Figure 6As shown, the transverse movement driving mechanism 5 comprises a rotary driving motor 531, a screw rod 53, a screw nut sliding block 51, a guide sliding block 52, a guide rod 54 and a connecting seat 55, the screw nut sliding block 51 is threadedly connected to the outside of the screw rod 53, and the screw rod 53 is connected with the rotating shaft of the rotary driving motor 531; specifically, the rotary driving motor 531 is installed on a support seat three 50, both ends of the screw rod 53 are respectively provided with bearings 501 and are supported by two support seats three 50, and the bearings 501 installed at both ends of the screw rod 53 are installed on the support seat three 50 for supporting the screw rod 53; the guide rod 54 is arranged parallel to the screw rod 53, both ends of the guide rod 54 are respectively fixed on the two support seats three 50, the guide sliding block 52 is slidingly installed on the outside of the guide rod 54 opposite to the screw nut sliding block 51, the connecting seat 55 is connected between the screw nut sliding block 51 and the guide sliding block 52, and the support seat three 50 is installed on the support table three 25.

[0067] Further, the rotation of the rotary driving motor 531 in the embodiment controls the rotation of the screw rod 53, the moving position of the transversely movable liquid adding seat and the input of the detection liquid into the incubation tank 331 in turn, and the rotary driving motor 531 is electrically connected with the control unit; under the rotary driving of the rotary driving motor 531, the screw nut sliding block 51 moves along the length direction of the screw rod 53, the guide sliding block 52 moves along the length direction of the guide rod 54 synchronously with the screw nut sliding block 51, and further drives the connecting seat 55 to move transversely. Further, the transverse movement driving mechanism 5 can also adopt other displacement driving devices.

[0068] Further, as shown in Figure 3 , Figure 5 and Figure 6 , the transversely movable liquid adding seat comprises a hanging support plate two 59, the rear end of the hanging support plate two 59 is fixed on the connecting seat 55 by bolts, the front end of the hanging support plate two 59 is provided with a plurality of mounting through holes one, a plurality of liquid outlet nozzles 591 are installed in the plurality of mounting through holes one, the upper end of each of the liquid outlet nozzles 591 is used for being connected with a detection liquid output pipe capable of moving with the hanging support plate two 59, and the lower end of each of the liquid outlet nozzles 591 is provided with a liquid outlet; under the rotary driving of the rotary driving motor 531, the plurality of liquid outlet nozzles 591 can be moved to the upper side of the incubation tank 331 in turn; further, the front end of the hanging support plate two 59 is provided with a plurality of conveying pipe limiting protrusions 592, and the output end of the detection liquid output pipe can be clamped between two adjacent conveying pipe limiting protrusions 592, so as to conveniently limit the output end of the detection liquid output pipe.

[0069] Further, as shown in Figure 3 , Figure 5 and Figure 6As shown, the delivery pump in the embodiment is a peristaltic pump 27, the peristaltic pump 27 is installed on the base 2, the elastic hose 271 connected between the detection liquid input pipe and the detection liquid output pipe is installed on the rotating extrusion head provided on the peristaltic pump 27, and the rotating extrusion head intermittently extrudes the elastic hose 271 to realize input liquid during rotation. Further, the peristaltic pump 27 is connected with the control unit, and the peristaltic pump 27 is controlled by the control unit; further, the specific structure and working principle of the peristaltic pump 27 can refer to the prior art in the field, and will not be described here. In addition, the liquid adding device can also use other liquid adding equipment.

[0070] Further, as shown in Figure 3 , Figure 5 and Figure 6 , the liquid suction device in the embodiment includes a lifting device, a liquid suction pump, a liquid suction pipe, a liquid suction nozzle 581, and a hanging support plate one 58. The lifting device is installed on the base 2. The hanging support plate one 58 has a bent structure. The rear end of the hanging support plate one 58 is fixed on the lifting drive device by bolts. The front end of the hanging support plate one 58 extends forward and bends downward to form an extension end. The extension end of the hanging support plate one 58 is provided with a mounting through hole two. The liquid suction nozzle 581 is installed in the mounting through hole two. The upper end of the liquid suction nozzle 581 is connected with the liquid suction pipe which can move with the hanging support plate one 58. The lower end of the liquid suction nozzle 581 is provided with a liquid suction port. The liquid suction pump is connected with the liquid suction pipe. The output end of the liquid suction pipe is used to be connected with a waste liquid pool. The waste liquid pool can be arranged on one side of the base 2 to store waste liquid. Further, the lifting drive device is an electric push rod three 57. The electric push rod three 57 is installed on a support seat four 56. The front side of the support seat four 56 is provided with a connecting lug 561. The connecting lug 561 is installed on the rear end of the connecting seat 55 by bolts. The rear end of the hanging support plate one 58 is connected with the movable push rod on the electric push rod three 57.

[0071] Further, under the rotation drive of the rotation drive motor 531, the liquid suction nozzle 581 can be moved to above the incubation groove 331 in sequence. Under the lifting drive of the electric push rod three 57, the liquid suction nozzle 581 is lifted. In addition, the electric push rod three 57 and the liquid suction pump are connected with the control unit, and the electric push rod three 57 is controlled by the control unit. It should be noted that the liquid suction pump and the liquid suction pipe are not shown. The specific arrangement mode of the liquid suction pump and the liquid suction pipe can be various. In addition, the liquid suction device can also use other liquid suction equipment.

[0072] Further, as shown in Figures 2 to 5 , the right end of the support table two 21 is provided with a support block 23. The upper end of the support block 23 is provided with a cleaning pool 24. The liquid suction nozzle 581 can be moved to above the cleaning pool 24 and is driven by the electric push rod three 57 to extend into the bottom of the cleaning pool 24 to suck liquid in the cleaning pool 24.

[0073] In the embodiment, as shown in Figure 5 and Figure 7 , the film strip incubation device further comprises: a hanging rack arranged above the incubation disc placing portion; the air outlet structure 9 comprises: a plurality of air outlet nozzles 96, the plurality of air outlet nozzles 96 are arranged on the hanging rack at intervals, and the air outlets of the air outlet nozzles 96 face the incubation grooves 331; this is conducive to the flow of the detection liquid in the plurality of incubation grooves 331, and can also improve the uniformity of the air outlet structure 9 blowing the incubation grooves 331, which is conducive to improving the consistency of the disturbance of the detection liquid in the plurality of incubation grooves 331.

[0074] In the embodiment, as shown in Figure 5 and Figure 7 , the plurality of air outlet nozzles 96 are respectively connected with the air fan through the air conveying pipeline, and the plurality of air outlet nozzles 96 are arranged on the hanging rack at intervals. Further, the air outlet nozzles 96 are detachably arranged on the hanging rack, and the direction of the air outlet of the air outlet nozzle 96 is adjustable; in addition, the air conveying pipeline and the air fan in the embodiment are not shown.

[0075] Further, as shown in Figure 5 and Figure 7 , the air outlet structure 9 further comprises: a plurality of sliding mounting seats 95 and locking pieces two, the plurality of sliding mounting seats 95 are provided one by one corresponding to the plurality of air outlet nozzles 96, the sliding mounting seats 95 are respectively slidably arranged on the transverse support rods, and the air outlet nozzles 96 are arranged on the sliding mounting seats 95; the plurality of locking pieces two are provided one by one corresponding to the plurality of sliding mounting seats 95, the locking pieces two are connected with the sliding mounting seats 95 and can lock the sliding mounting seats 95 on the transverse support rods. In the embodiment, the spacing between the adjacent two sliding mounting seats 95 in the transverse direction is adjusted, so that the spacing between the plurality of air outlet nozzles 96 in the transverse direction is adjusted; in addition, the sliding mounting seats 95 are locked by the locking pieces two, which is conducive to fixing the arrangement positions of the sliding mounting seats 95 and the air outlet nozzles 96.

[0076] Further, as shown in Figure 5 and Figure 7 , the air inlet end of the air outlet nozzle 96 is provided with a connecting portion for connecting with the air conveying pipe, and the air inlet is arranged on the connecting portion; further, one side of the sliding mounting seat 95 is provided with a mounting protrusion 951, and the mounting hole is arranged on the mounting protrusion 951, and the air outlet nozzle 96 is arranged in the mounting hole, specifically, the air outlet nozzle 96 is arranged in the mounting hole in an interference fit; in addition, the air outlet nozzle 96 can also be arranged on the sliding mounting seat 95 by other mounting modes.

[0077] Further, as shown in Figure 5 and Figure 7As shown, the locking member two is a screw bolt five 97, one side of the sliding mounting seat 95 is provided with a connecting protrusion 952, the connecting protrusion 952 is provided with a threaded hole in communication with the mounting opening, the screw bolt five 97 is threadedly connected in the threaded hole, by tightening the screw bolt five 97, the screw-in end of the screw bolt five 97 is in abutment with the transverse support rod and locks the sliding mounting seat 95; while by loosening the screw bolt five 97, the position of the sliding mounting seat 95 can be adjusted by adjusting the sliding of the sliding mounting seat 95 along the transverse support rod, and the orientation of the air outlet nozzle 96 can also be adjusted by circumferentially rotating the sliding mounting seat 95.

[0078] In the embodiment, as shown in Figure 5 and Figure 7 , the suspension frame comprises a support structure, a transverse support rod one 92 and a transverse support rod two 94, the transverse support rod one 92 is connected to the support structure, and a plurality of air outlet nozzles 96 are slidingly mounted on the transverse support rod one 92 at intervals; the transverse support rod two 94 is connected to the support structure and is arranged in parallel and at intervals with the transverse support rod one 92, and a plurality of air outlet nozzles 96 are slidingly mounted on the transverse support rod two 94 at intervals.

[0079] In the embodiment, as shown in Figures 2 to 5 , a transparent light shield 8 is provided in the embodiment, the transparent light shield 8 is mounted on the electric push rod one 7, and the support structure is specifically a connecting support beam 90, which is horizontally arranged and mounted on the top plate of the transparent light shield 8; the transverse support rod one 92 is connected to the lower end of the connecting support beam 90 through an inclined connecting block one 91, and the left and right ends of the transverse support rod one 92 extend horizontally to form free ends; in addition, the transverse support rod two 94 is connected to the lower end of the connecting support beam 90 through an inclined connecting block two 93, and the left and right ends of the transverse support rod two 94 extend horizontally to form free ends.

[0080] Further, as shown in Figures 2 to 5 , a plurality of bolt holes one 901 are arranged at intervals on the connecting support beam 90 in the embodiment, and a plurality of bolt holes two are arranged on the transparent light shield 8 one by one corresponding to the plurality of bolt holes one 901, and the transparent light shield 8 is fixed on the connecting support beam 90 by the fixing bolt one 82 and the fixing nut one 83 in the embodiment. Further, the suspension frame can also be provided in other structures.

[0081] Further, as shown in Figures 2 to 5As shown, the air inlet pipe 80 in the air conveying pipeline for conveying air to the plurality of air outlets 96 in the embodiment passes through the top plate of the transparent light shield 8, and the air outlet pipe 81 for air exhaust passes through the rear wall of the transparent light shield 8, so that the gas in the transparent light shield 8 can be discharged and treated through the air outlet pipe 81. It should be noted that the air inlet pipe 80 and the air outlet pipe 81 are flexible pipes and can move with the transparent light shield 8. In addition, the air inlet pipe 80 and the air outlet pipe 81 are not completely shown, and the fan, the air inlet pipe 80 and the air outlet pipe 81 can be arranged and connected in the manner known in the art.

[0082] In one embodiment of the present application, the membrane strip incubation device further comprises a control unit, and the liquid adding device, the liquid sucking device and the air blowing disturbance device are electrically connected to the control unit, so as to automatically input the detection liquid into the incubation groove 331, automatically suck out the detection liquid in the incubation groove 331, automatically control the air blowing speed and the air volume to flexibly adjust the disturbance degree of the detection liquid in the incubation groove 331, and automatically switch the air blowing mode of the air blowing disturbance device. It should be noted that the specific structure of the control unit can refer to the prior art in the art, and will not be described here.

[0083] In the embodiment, as shown in Figures 2 to 5 The right side of the support table 20 is provided with a control operation table 22, and the control operation table 22 is provided with a touch screen connected to the control unit, so as to control and operate the membrane strip incubation device. Further, the bottom of the base 2 is provided with a plurality of support legs 28 for supporting the base 2.

[0084] In one embodiment of the present application, as shown in Figures 2 to 5 The membrane strip incubation method for Western blotting further comprises that a transparent light shield 8 is covered on the outer periphery of the base 2, so that the incubation environment is isolated from the outside.

[0085] In the embodiment, as shown in Figures 2 to 5 The transparent light shield 8 is used to isolate the incubation environment from the outside, so as to form a relatively closed space in the transparent light shield 8, reduce or avoid the influence of external airflow on the space in the transparent light shield 8, and improve the disturbance effect of the air outlet structure 9 provided on the air blowing disturbance device on the liquid in the incubation groove 331. Further, the transparent light shield 8 can shield light and develop color for the membrane strip 47 during the incubation of the membrane strip 47.

[0086] In the embodiment, as shown in Figures 2 to 5As shown, the film strip incubation device also includes a detection workbench 1, the base 2 is placed on the detection workbench 1, and the upper side of the detection workbench 1 is provided with a receiving groove 10 capable of receiving the lower end surface profile of the transparent light shield 8, the lower end surface profile of the transparent light shield 8 is extended into the receiving groove 10, and the transparent light shield 8 forms a closed space; further, in order to improve the air isolation effect between the lower end surface profile of the transparent light shield 8 and the receiving groove 10, a sealing strip can also be installed in the receiving groove 10, and the sealing effect is improved by extruding the lower end of the transparent light shield 8 with the sealing strip.

[0087] An embodiment of the present application is as shown in Figures 2 to 5 The film strip incubation method for Western blotting also includes: driving the transparent light shield 8 to move up and down in the vertical direction through the first lifting driving device, the first lifting driving device is arranged outside the base 2, and the transparent light shield 8 is installed on the first lifting driving device.

[0088] In the present embodiment, as shown in Figures 2 to 5 The transparent light shield 8 is driven to move up and down by the first lifting driving device, the transparent light shield 8 is moved downward and covers the outer periphery of the base 2, and the transparent light shield 8 is moved upward to expose the base 2.

[0089] In the present embodiment, as shown in Figures 2 to 5 The film strip incubation device also includes the transparent light shield 8 and the first lifting driving device, the first lifting driving device is arranged outside the base 2, the transparent light shield 8 is installed on the first lifting driving device and moves up and down in the vertical direction under the driving of the first lifting driving device.

[0090] In the present embodiment, as shown in Figures 2 to 5As shown, the lifting driving device one comprises: an electric push rod one 7, which is fixedly arranged, and a movable push rod one 70 provided on the electric push rod one 7 extends vertically upward to form an extension end; in this embodiment, the electric push rod one 7 is installed on a support seat one 6, which is fixedly arranged on the rear side of the base 2, and the support seat one 6 comprises a support base 60 and a vertical support plate 61 vertically connected to the support base 60, and the front end of the vertical support plate 61 is connected with a support protrusion one 611 and a support protrusion two 612, and the middle part of the support protrusion one 611 and the support protrusion two 612 is respectively provided with a vertical through groove; further, the outer side wall of the electric push rod one 7 is respectively connected with a mounting plate one 71 and a mounting plate two 72 corresponding to the support protrusion one 611 and the support protrusion two 612, the mounting plate one 71 is fixedly connected with the support protrusion one 611 through bolts, the mounting plate two 72 is fixedly connected with the support protrusion two 612 through bolts, and the body of the electric push rod one 7 passes through the vertical through grooves provided on the support protrusion one 611 and the support protrusion two 612. Further, the support seat one 6 can also be arranged in other structures, and the electric push rod one 7 can also be installed on the support seat one 6 through other mounting modes.

[0091] Further, as shown in the drawings, Figures 2 to 5 In this embodiment, the lifting connection stringer 73 connected to the upper end of the movable push rod one 70, the lifting connection stringer 73 extends forward to form an extension end, the extension end of the lifting connection stringer 73 is connected with a hanging cross beam 74, the hanging cross beam 74 is provided with a plurality of bolt through holes three at intervals, the top plate of the transparent light shield 8 is provided with a plurality of bolt through holes four corresponding to the plurality of bolt through holes three provided on the hanging cross beam 74, and in this embodiment, the transparent light shield 8 is hung and installed on the hanging cross beam 74 by passing the fixed bolt one 82 through the corresponding bolt through holes three and bolt through holes four and fixing by the fixed nut one 83. Further, the electric push rod one 7 is connected with the control unit, and the electric push rod one 7 is controlled by the control unit, and in addition, the lifting driving device one can also use other lifting equipment.

[0092] In one embodiment of the present application, as shown in the drawings, Figures 2 to 5 The membrane strip incubation method for Western blotting further comprises: driving the air outlet structure 9 provided on the air blowing disturbance device to move up and down in the vertical direction by the lifting driving device one, the air outlet structure 9 is installed in the transparent light shield 8, and the air blowing disturbance device further comprises a fan for inputting air, and the air outlet structure 9 is connected with the fan through an air conveying pipeline.

[0093] In this embodiment, as shown in the drawings, Figures 2 to 5As shown, the air outlet structure 9 provided on the air blowing disturbance device is driven to move up and down in the vertical direction by the lifting driving device I, so that the air outlet structure 9 can move synchronously in the vertical direction relative to the incubation disc placing part under the driving of the lifting driving device I, and the distance between the air outlet structure 9 and the incubation disc 33 can be adjusted, the disturbance degree of the air blowing to the liquid can be adjusted by adjusting the distance between the air outlet structure 9 and the incubation disc 33, and then the disturbance mode and the disturbance degree can be increased, and the disturbance effect of the air outlet structure 9 on the liquid in the incubation groove 331 is further improved.

[0094] Further, the fan in the embodiment is connected with the control unit, the operation mode of the fan is controlled by the control unit, different air blowing modes are realized, the air outlet size, the air outlet time and the air outlet time length can be automatically adjusted, and various air blowing modes such as intermittent air blowing and air blowing wind force change from small to large are realized.

[0095] In one embodiment of the application, the membrane strip incubation method for the Western blotting method further comprises: adjusting the temperature in the transparent light shield 8 by the temperature adjusting device according to the requirement of the membrane strip 47 on the incubation temperature, so that the temperature in the transparent light shield 8 and the temperature of the liquid in the incubation groove 331 meet the requirement of the membrane strip 47 on the incubation temperature.

[0096] In the embodiment, the temperature in the transparent light shield 8 is adjusted by the temperature adjusting device, so that the temperature in the transparent light shield 8 and the temperature of the liquid in the incubation groove 331 meet the requirement of the membrane strip 47 on the incubation temperature, the adaptability of the membrane strip incubation device and the membrane strip incubation method to different temperature environments is improved, and the normal incubation of the membrane strip 47 and the accuracy of the incubation of the membrane strip 47 are ensured under different temperature environments. In addition, the temperature adjusting device can refer to the prior art in the field, and will not be described here.

[0097] In one embodiment of the application, as shown in Figure 5 the membrane strip incubation method for the Western blotting method further comprises: after the liquid in the incubation groove 331 is absorbed, the liquid remaining in the incubation groove 331 is blown and dried by the air blowing disturbance device. In the embodiment, after the liquid in the incubation groove 331 is absorbed, the liquid remaining in the incubation groove 331 is blown and dried by blowing air to the incubation groove 331, so that the liquid remaining in the incubation groove 331 can be removed more thoroughly, and the accuracy of the incubation of the membrane strip 47 is further improved.

[0098] In one embodiment of the application, as shown in Figure 9 and Figure 5As shown, the membrane strip incubation method for Western blotting also includes: placing the incubation tray 33 through the incubation tray placing rack 3, the upper side of the incubation tray placing rack 3 is provided with an incubation tray placing part; supporting the incubation tray placing rack 3 through the multiple elastic support structures 31, the lower end of the multiple elastic support structures 31 is connected to the base 2 in the vertical direction, and the incubation tray placing rack 3 is connected to the upper end of the multiple elastic support structures 31; during the process of blowing by the blowing disturbance device to the incubation groove 331, under the blowing action of the blowing disturbance device, the elastic support structure 31 produces elastic deformation, and the incubation tray 33 placed on the incubation tray placing part shakes.

[0099] In the embodiment, as shown in Figure 2 , under the blowing action of the blowing disturbance device, the wind blows on the incubation tray 33, the wind force acts on the incubation tray 33, so that the elastic support structure 31 produces elastic deformation, and further makes the incubation tray 33 placed on the incubation tray placing part can shake, and the liquid in the incubation groove 331 has disturbance produced by the shaking of the incubation tray 33 while flowing relative to the membrane strip 47 placed in the incubation groove 331, which is beneficial to increase the disturbance mode and the disturbance degree, and further improve the disturbance effect of the air outlet structure 9 on the liquid in the incubation groove 331.

[0100] In the embodiment, as shown in Figure 5 , Figure 9 and Figure 3 , the membrane strip incubation device also includes the elastic support structure 31 and the incubation tray placing seat 32, the elastic support structure 31 is provided with multiple, the lower end of the multiple elastic support structures 31 is connected to the base 2 in the vertical direction; the incubation tray placing seat 32 is connected to the upper end of the multiple elastic support structures 31, the upper side of the incubation tray placing seat 32 is provided with an incubation tray placing part; the incubation tray 33 is placed in the incubation tray placing part, and under the blowing action of the blowing disturbance device, the elastic support structure 31 produces elastic deformation, and the incubation tray 33 placed on the incubation tray placing part can shake.

[0101] Further, as shown in Figure 9 and Figure 3 , the membrane strip incubation device also includes the mounting base 30, the mounting base 30 is installed on the support table two 21, the mounting base 30, the incubation tray placing seat 32 and the multiple elastic support structures 31 constitute the incubation tray placing rack 3, the multiple elastic support structures 31 are vertically connected between the mounting base 30 and the incubation tray placing seat 32, and the upper side of the incubation tray placing seat 32 is provided with an incubation tray placing groove.

[0102] In the embodiment, as shown in Figure 9 and Figure 3As shown in the drawings, the upper side of the mounting base 30 is provided with three horizontal guide rails one 301, and the lower side of the incubation tray placing seat 32 is provided with a plurality of horizontal guide rails two 321 opposite to the three horizontal guide rails one 301; the elastic support structure 31 comprises a sliding block one 311, a sliding block two 313 and a spring 312, the sliding block one 311 is slidingly installed on the horizontal guide rail one 301, the sliding block two 313 is slidingly installed on the horizontal guide rail two 321, and the spring 312 is connected between the sliding block one 311 and the sliding block two 313.

[0103] In this embodiment, as shown in the drawings, Figure 9 、 Figure 11 and Figure 3 , the sliding block one 311 is slidingly installed on the horizontal guide rail one 301, the horizontal guide rail one 301 extends into the sliding groove one 3111 provided on the sliding block one 311, the sliding block two 313 is slidingly installed on the horizontal guide rail two 321, and the horizontal guide rail two 321 extends into the sliding groove two 3131 provided on the sliding block two 313, so as to facilitate the adjustment of the position of the elastic support structure 31, thereby facilitating the adjustment of the distance between the plurality of elastic support structures 31 and the adjustment of the shaking degree of the incubation tray placing seat 32 under the disturbance of the disturbance driving device, so that the flow degree of the detection liquid relative to the membrane strip 47 placed in the incubation groove 331 is suitable.

[0104] Further, as shown in the drawings, Figure 9 、 Figure 11 and Figure 3 , the incubation tray placing seat 32 in this embodiment is horizontally installed at the upper end of the plurality of elastic support structures 31, and the horizontal guide rail two 321 is vertically connected to the lower side of the incubation tray placing seat 32; further, the elastic support structure 31 in this embodiment can also use other elastic support components.

[0105] In this embodiment, as shown in the drawings, Figure 9 、 Figure 11 and Figure 9 , the incubation tray placing rack 3 further comprises a screwing member one and a screwing member two, a plurality of screwing member ones and a plurality of screwing member twos are respectively provided corresponding to the plurality of elastic support structures 31, the screwing member one is connected with the sliding block one 311 and locks the sliding block one 311 on the horizontal guide rail one 301, and the screwing member two is connected with the sliding block two 313 and locks the sliding block two 313 on the horizontal guide rail two 321.

[0106] Further, the first screwing part is a first screwing bolt 314, one side of the first sliding block 311 is provided with a second threaded hole, the first screwing bolt 314 is threadedly connected with the second threaded hole and the screwing-in end of the first screwing bolt 314 extends into the first sliding groove 3111, the first screwing bolt 314 is tightened, the screwing-in end of the first screwing bolt 314 abuts against the first horizontal guide sliding rail 301 to lock the first sliding block 311 on the first horizontal guide sliding rail 301, and the first screwing bolt 314 is loosened to enable the first sliding block 311 to slide along the length direction of the first horizontal guide sliding rail 301; the second screwing part is a second screwing bolt 315, one side of the second sliding block 313 is provided with a third threaded hole, the second screwing bolt 315 is threadedly connected with the third threaded hole and the screwing-in end of the second screwing bolt 315 extends into the second sliding groove 3131, the second screwing bolt 315 is tightened, the screwing-in end of the second screwing bolt 315 abuts against the second horizontal guide sliding rail 321 to lock the second sliding block 313 on the second horizontal guide sliding rail 321, and the second screwing bolt 315 is loosened to enable the second sliding block 313 to slide along the length direction of the second horizontal guide sliding rail 321.

[0107] In the embodiment, as shown in Figure 9 , the upper side of the incubation tray placing seat 32 is provided with an incubation tray placing groove, the rear end of the incubation tray placing groove is provided with a first stop edge 322, the front end of the incubation tray placing groove is provided with a second stop edge 323, and the incubation tray placing rack 3 further comprises a plurality of third screwing parts, a plurality of threaded holes are arranged on the second stop edge 323 along the length direction of the second stop edge 323, and the plurality of third screwing parts are threadedly connected with the plurality of threaded holes respectively, and the screwing-in ends of the third screwing parts can extend into the incubation tray placing groove and abut against the incubation tray 33.

[0108] In addition, as shown in Figure 2 , the third screwing part in the embodiment is a fourth screwing bolt 3231, the threaded hole arranged on the second stop edge 323 is a fourth threaded hole, the fourth screwing bolt 3231 is threadedly connected with the fourth threaded hole and the screwing-in end of the fourth screwing bolt 3231 extends into the incubation tray placing groove, the fourth screwing bolt 3231 is tightened, the fourth screwing bolt 3231 abuts against the incubation tray 33 to lock the incubation tray 33 in the incubation tray placing groove.

[0109] Further, as shown in Figure 9 , Figure 5 , three incubation trays 33 are placed in the incubation tray placing groove shown in the embodiment, the incubation trays 33 are locked by the fourth screwing bolt 3231, and in addition, two or four incubation trays 33 can be placed according to requirements.

[0110] An embodiment of the present application, as shown in Figure 8 and Figure 5 , before the membrane strips 47 to be detected are placed into the incubation grooves 331 respectively, the membrane strip incubation method for the Western blotting further comprises:

[0111] A plurality of membrane strips 47 are respectively placed on the membrane strip placing rack 4, which is arranged on one side of the base 2 or mounted on the base 2 and located above the incubation tray placing part. The plurality of incubation grooves 331 of the incubation tray 33 placed on the incubation tray placing part are respectively provided with a plurality of placing structures 46, and the placing structures 46 are respectively provided with placing parts for detachably placing the membrane strips 47.

[0112] The height position of the membrane strip placing rack 4 in the vertical direction is adjusted by the second lifting driving device. The membrane strip placing rack 4 is mounted on the second lifting driving device and can be lifted in the vertical direction under the driving of the second lifting driving device. The placing parts and the membrane strips 47 placed on the placing parts can be extended into the incubation grooves 331 under the driving of the second lifting driving device.

[0113] In the embodiment, as shown in Figure 8 and Figure 5 , the membrane strip placing rack 4 is provided with a plurality of placing structures 46, and the placing structures 46 are respectively provided with placing parts for detachably placing the membrane strips 47. The membrane strips 47 can be placed on the placing parts, and the membrane strips 47 can be limited by the placing parts to avoid being turned up during incubation. Further, the height position of the membrane strip placing rack 4 in the vertical direction is adjusted by the second lifting driving device. The membrane strip placing rack 4 can be lifted by the second lifting driving device, so that the membrane strips 47 placed on the placing parts can be extended into the incubation grooves 331 and taken out of the incubation grooves 331, and the membrane strips 47 can be disassembled and assembled.

[0114] In the embodiment, as shown in Figure 8 and Figure 5 , the membrane strip incubation device further comprises the second lifting driving device and the membrane strip placing rack 4. The second lifting driving device is mounted on the base 2, and the membrane strip placing rack 4 is mounted on the second lifting driving device and located above the incubation tray placing part. The plurality of incubation grooves 331 of the incubation tray 33 placed on the incubation tray placing part are provided with a plurality of placing structures 46, and the placing structures 46 are respectively provided with placing parts for detachably placing the membrane strips 47. The membrane strip placing rack 4 can be lifted in the vertical direction under the driving of the second lifting driving device, and the placing parts and the membrane strips 47 placed on the placing parts can be extended into the incubation grooves 331 under the driving of the second lifting driving device.

[0115] In the embodiment, as shown in Figure 8 and Figure 4As shown in the figure, the film strip shelf 4 in the embodiment comprises: two longitudinal support beams 43, which are parallel and spaced apart in the transverse direction and horizontally extend in the longitudinal direction, two guiding slide rails 431 are connected to the two longitudinal support beams 43 respectively, two support sliding blocks 44 are provided and are slidingly installed on the guiding slide rails 431 respectively, and a suspension support beam 45 is connected between the two support sliding blocks 44; a plurality of shelf structures 46 are detachably connected to the suspension support beam 45 in the transverse direction. In addition, the suspension support beam 45 in the embodiment is provided in two, and the two suspension support beams 45 are parallel and spaced apart in the longitudinal direction.

[0116] In the embodiment, as shown in the figure, Figure 8 and Figure 8 The film strip shelf 4 further comprises: a plurality of locking members one, which are connected to the support sliding blocks 44 respectively and can lock the support sliding blocks 44 on the guiding slide rails 431 respectively; further, the locking member one in the embodiment is specifically a screw bolt three 441, two threaded holes one are provided on the two support sliding blocks 44 respectively, the threaded holes one are communicated with the sliding grooves in the support sliding blocks 44, the screw bolt three 441 is threadedly connected with the threaded holes one, and the screw-in end of the screw bolt three 441 can be in abutment with the guiding slide rails 431 to lock the support sliding blocks 44; in addition, the screw bolt three 441 can be loosened to take out the two support sliding blocks 44 and the suspension support beam 45 upward.

[0117] In the embodiment, as shown in the figure, Figure 10 and Figure 10 The shelf structure 46 comprises: a shelf body 461, the upper end of the shelf body 461 is provided with a hook structure for being detachably suspended on the support body, the hook structure is detachably suspended on the suspension support beam 45, and the lower end of the shelf body 461 is provided with a shelf part.

[0118] In the embodiment, as shown in the figure, Figure 10 The shelf body 461 is in a bent structure, the inner side of the shelf body 461 forms a clamping groove in a U-shaped structure, the width direction cross section of the suspension support beam 45 is in a rectangular shape, the suspension support beam 45 is clamped in the clamping groove in the shelf body 461, the lower end of the shelf body 461 is connected with a horizontally extending shelf protrusion 462, and a shelf groove 4621 is opened on the shelf protrusion 462. Further, the material of the shelf body 461 is an elastic material, the hook structure is suspended on the suspension support beam 45, the shelf body 461 is elastically deformed and presses the suspension support beam 45, in addition, the shelf body 461 can also be provided in other structures, and the shelf part can also be provided in other structures, which is convenient for the film strip 47 to be shelved.

[0119] In the embodiment, as shown in the figure, Figure 5As shown, the rest part is a rest groove 4621, which is horizontally arranged at the lower end of the rest structure 46 and has three open sides; it is convenient to insert the film strip 47 into the rest groove 4621, and it is beneficial to make the two ends of the film strip 47 pass through the rest groove 4621 respectively, so as to adjust the rest position of the film strip 47 and the rest groove 4621, and make the film strip 47 horizontally rest in the rest groove 4621.

[0120] In the embodiment, as shown in Figure 10 and Figure 8 , the rear ends of the two longitudinal support beams 43 in the embodiment are connected with the lifting connecting beam 42, and the lifting connecting beam 42 connects the two longitudinal support beams 43 into one body; further, the longitudinal support beam 43 in the embodiment has a uniform long strip structure, and the two suspension support beams 45 in the embodiment have a bent structure, and the left and right ends of the two suspension support beams 45 are respectively provided with horizontal connecting sections, and the middle part of the suspension support beam 45 is provided with a horizontally arranged suspension section, and the left and right ends of the suspension section are connected with the horizontal connecting sections through vertical connecting sections, and the suspension section is located at the lower side of the horizontal connecting section.

[0121] It should be noted that, as shown in Figure 10 and Figure 5 , the film strip 47 has a long strip structure, and a plurality of samples 471 are arranged on the film strip 47 at intervals, and when the film strip 47 is horizontally rested in the two oppositely arranged rest grooves 4621, the samples 471 arranged on the film strip 47 at intervals are staggered with the rest grooves 4621 respectively; in addition, the depth of the vertical direction groove depth of the rest groove 4621 is slightly greater than the thickness of the film strip 47.

[0122] In the embodiment, as shown in Figure 5 , the lifting driving device two in the embodiment comprises: an electric push rod two 41, which is fixedly arranged, and an active push rod two 411 provided on the electric push rod two 41 extends vertically upward to form an extension end, and the film strip rest rack 4 is horizontally connected to the extension end of the active push rod two 411.

[0123] In the embodiment, as shown in ​ , the electric push rod two 41 in the embodiment is provided with two, and the two electric push rod two 41 are respectively installed on the support seat two 40, and the two support seat two 40 are installed on the left and right ends of the rear side of the base 2, and the left and right ends of the lifting connecting beam 42 are connected to the active push rod two 411 of the two electric push rod two 41. Further, the electric push rod two 41 in the embodiment is connected with the control unit, and the electric push rod two 41 is controlled through the control unit; further, the lifting driving device two can also use other lifting devices, which is convenient to adjust the height position of the film strip rest rack 4 by lifting driving the film strip rest rack 4; in addition, the film strip rest rack 4 can also be arranged in other structures.

[0124] In addition, the detection method for the Western blotting method provided in the embodiment comprises: the film strip incubation method for the Western blotting method; and air blowing is performed on the film strip 47 after the incubation is completed by using the air blowing disturbance device, so that the film strip 47 is dried by air.

[0125] In the embodiment, the detection method for the Western blotting method facilitates the film strip 47 in the incubation tank 331 to be fully contacted with the liquid and to fully react, and improves the incubation effect of the film strip 47; in addition, the disturbance degree of the liquid in the incubation tank 331 can be flexibly adjusted by controlling the air blowing speed and the air volume, so that the disturbance of the liquid is suitable, and the liquid is prevented from overflowing out of the incubation tank 331 in the incubation process; further, the film strip 47 after the incubation is completed is dried by air blowing through the air blowing disturbance device, the uniformity of the drying of the film strip 47 is improved, and the film strip 47 after the drying is taken out and subjected to the next detection operation.

[0126] In an embodiment of the present application, in the process of air blowing on the film strip 47 after the incubation is completed by using the air blowing disturbance device, the detection method for the Western blotting method further comprises: the air in the air conveying pipeline is heated by turning on the air heating device; the air blowing disturbance device comprises an air blower and an air conveying pipeline, the air blower is arranged on one side of the base 2 or is mounted on the base 2 and is used for inputting air; the air conveying pipeline is connected to the air outlet end of the air blower, the air outlet structure 9 is connected to the air outlet end of the air conveying pipeline and is arranged above the incubation disc placing part, and the air heating device is mounted in the air conveying pipeline or is arranged on the outer periphery of the air conveying pipeline.

[0127] In the embodiment, the air in the air conveying pipeline is heated by turning on the air heating device, the air in the air conveying pipeline is heated by using the air heating device, the air in the air conveying pipeline is heated after the incubation of the film strip 47 is completed, then hot air is blown on the film strip 47 to dry the film strip 47, the film strip 47 is dried, and the film strip 47 after the drying is taken out and subjected to the next detection operation; further, the film strip 47 is dried by blowing hot air on the film strip 47, the film strip 47 is fully dried, and the efficiency and the uniformity of the drying are improved.

[0128] In the embodiment, the air heating device arranged on the film strip incubation device can be mounted in the air conveying pipeline or can be arranged on the outer periphery of the air conveying pipeline. Further, the air heating device is connected to the control unit and is controlled by using the control unit; in addition, the air heating device can be an electric heating wire, the electric heating wire is mounted in the air conveying pipeline, and the electric heating wire is not shown in the figure.

[0129] It should be noted that the membrane strip incubation device in each of the above embodiments is specifically an immunoblotting instrument; further, the membrane strip incubation device in each of the above embodiments is only one of various membrane strip incubation devices or immunoblotting instruments capable of the above membrane strip incubation method; further, other operations of the above membrane strip incubation method and other procedures of the detection method for the Western blotting method in the embodiments can refer to existing incubation technologies in the art, which will not be described here in detail.

[0130] In addition, in addition to the technical solutions disclosed in the embodiments, the rotating drive motor 531, the electric push rod, the membrane strip 47, the control unit, other components of the membrane strip incubation device, and the working principles thereof in the present application can refer to conventional technical solutions in the technical field, and these conventional technical solutions are not the focus of the present application, which will not be described in detail herein.

[0131] In the present application, the term "a plurality of" refers to two or more, unless otherwise explicitly limited. The terms "mounting", "connecting", "connecting", "fixing" and the like should be understood in a broad sense, for example, "connecting" can be fixed connection, or detachable connection, or integrally connected; "connected" can be directly connected, or indirectly connected through an intermediate medium. For those skilled in the art, the specific meaning of the above terms in the present application can be understood according to the specific circumstances.

[0132] In the description of the present application, it should be understood that the terms "upper", "lower", "front", "rear" and the like indicate the orientation or positional relationship based on the orientation or positional relationship shown in the drawings, and are only for the convenience of describing the present application and simplifying the description, and do not indicate or imply that the devices or units referred to must have a particular direction, be constructed and operated in a particular orientation, therefore, it cannot be understood as a limitation on the present application.

[0133] In the description of the present application, the terms "one embodiment", "some embodiments", "a specific embodiment" and the like mean that the specific features, structures, materials or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the present application. In the present application, the illustrative description of the above terms does not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described can be combined in any one or more embodiments or examples in a suitable manner.

[0134] The above is only the preferred embodiment of the present application and is not used to limit the present application. For those skilled in the art, the present application can have various changes and modifications. Any modification, equivalent replacement, improvement, etc. made within the spirit and principles of the present application shall be included in the protection scope of the present application.

Claims

1. A method for membrane strip incubation for Western blotting, characterized in that, The application relates to a membrane strip incubation device. The membrane strip is incubated by using a membrane strip incubation device, the incubation device comprises a base and an incubation disc, the base is provided with an incubation disc placing part, the incubation disc is horizontally placed on the incubation disc placing part, and a plurality of incubation grooves for incubating the membrane strip are arranged on the incubation disc at intervals. The membrane strip to be detected is placed in the incubation grooves respectively. Buffer washing liquid and blocking liquid are added into the incubation grooves respectively, the membrane strip is wetted, the liquid in the incubation groove is blown to flow, and membrane blocking is carried out for a first time length. The sample to be detected is added into the incubation grooves respectively, the liquid in the incubation groove is blown to flow, sample incubation is carried out for a second time length, and then the liquid in the incubation groove is sucked dry. Buffer washing liquid is added into the incubation grooves respectively, the liquid in the incubation groove is blown to flow, membrane washing is carried out for a third time length, and then the buffer washing liquid in the incubation groove is sucked dry. Buffer washing liquid and enzyme combination are added into the incubation grooves respectively, the liquid in the incubation groove is blown to flow, enzyme combination is carried out for a fourth time length, and then the liquid in the incubation groove is sucked dry. Buffer washing liquid is added into the incubation grooves respectively, the liquid in the incubation groove is blown to flow, membrane washing is carried out for a fifth time length, and then the buffer washing liquid in the incubation groove is sucked dry. Distilled water and chromogenic liquid are added into the incubation grooves respectively, the membrane strip is shaded, the liquid in the incubation groove is blown to flow, light-proof chromogenic is carried out for a sixth time length, and then the liquid in the incubation groove is sucked dry. Distilled water is added into the incubation grooves respectively, the liquid in the incubation groove is blown to flow, chromogenic termination is carried out for a seventh time length, and then the distilled water in the incubation groove is sucked dry. The liquid in the incubation groove is blown to flow by blowing the air disturbance device to the incubation groove and making the liquid in the incubation groove flow relative to the membrane strip placed in the incubation groove.

2. The membrane strip incubation method for Western blotting according to claim 1, characterized in that, Further comprising: A transparent light shield cover is arranged on the outer periphery of the base, so that the incubation environment is isolated from the outside world.

3. The membrane strip incubation method for Western blotting according to claim 2, characterized in that, Further comprising: The transparent light shield cover is driven to move up and down in the vertical direction by the lifting driving device one, the lifting driving device one is arranged outside the base, and the transparent light shield cover is mounted on the lifting driving device one.

4. The membrane strip incubation method for Western blotting according to claim 3, characterized in that, Further comprising: The air outlet structure arranged on the air disturbance device is driven to move up and down in the vertical direction by the lifting driving device one, the air outlet structure is mounted in the transparent light shield cover, the air disturbance device further comprises an air fan for inputting air, and the air outlet structure is connected with the air fan through an air conveying pipeline.

5. The membrane strip incubation method for Western blotting according to claim 2, characterized in that, Further comprising: According to the requirement of the membrane strip on the incubation temperature, the temperature in the transparent light shield cover is adjusted by the temperature adjusting device, so that the temperature in the transparent light shield cover and the temperature of the liquid in the incubation groove meet the requirement of the membrane strip on the incubation temperature.

6. The membrane strip incubation method for Western blotting according to any one of claims 1 to 5, characterized in that, Further comprising: During the incubation of the membrane strip, after the liquid in the incubation groove is sucked dry, the air disturbance device blows air to the incubation groove and makes the residual liquid in the incubation groove dry.

7. The membrane strip incubation method for Western blotting according to any one of claims 1 to 5, characterized in that, Further comprising: The incubation disc is placed by using an incubation disc placing rack, and the upper side of the incubation disc placing rack is provided with the incubation disc placing part. The incubation tray rack is supported by a plurality of elastic support structures arranged at intervals, the lower ends of the plurality of elastic support structures are connected to the base in the vertical direction, and the incubation tray rack is connected to the upper ends of the plurality of elastic support structures; During the blowing process of the blowing disturbance device to the incubation tank, the elastic support structure is elastically deformed under the blowing action of the blowing disturbance device, and the incubation tray placed on the incubation tray placing part shakes.

8. The membrane strip incubation method for Western blotting according to any one of claims 1 to 5, characterized in that, Before placing the membrane strips to be detected into the incubation tanks, further comprising: A plurality of membrane strips are placed on the membrane strip placing rack, the membrane strip placing rack is arranged on one side of the base or mounted on the base, the membrane strip placing rack is located above the incubation tray placing part, a plurality of placing structures are detachably provided on the plurality of incubation tanks of the incubation tray placed on the incubation tray placing part, and a placing part for detachably placing the membrane strip is arranged on each placing structure. The height position of the membrane strip placing rack in the vertical direction is adjusted by the second lifting driving device, the second lifting driving device is mounted on the base, the membrane strip placing rack is mounted on the second lifting driving device and can move up and down in the vertical direction under the driving of the second lifting driving device, and the placing part and the membrane strip placed on the placing part can extend into the incubation tank under the driving of the second lifting driving device.

9. A detection method for Western blotting, characterized by, Comprise: The membrane strip incubation method for Western blotting method according to any one of claims 1 to 8; The membrane strips are blown by the blowing disturbance device to complete the incubation, so that the plurality of membrane strips are air-dried.

10. The detection method for Western blotting according to claim 9, characterized in that, During the blowing process of the blowing disturbance device to the incubation tank, further comprising: The air in the air conveying pipeline is heated by turning on the air heating device, the blowing disturbance device comprises a fan and an air conveying pipeline, the fan is arranged on one side of the base or mounted on the base and used for inputting air, the air conveying pipeline is connected to the air outlet end of the fan, the air outlet structure is connected to the air outlet end of the air conveying pipeline and arranged above the incubation tray placing part, and the air heating device is mounted in the air conveying pipeline or arranged on the outer periphery of the air conveying pipeline.

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