A method for breeding artificial seedlings of holothuria scabra

Abstract

The present application belongs to the field of sea cucumber breeding, and particularly relates to a breeding method of artificial seedlings of Apostichopus japonicus, which greatly improves the success rate of inducing spawning and the spawning amount of parent sea cucumber by improving the feed structure of parent sea cucumber; by putting Enterococcus faecalis, Bacillus licheniformis and Lactobacillus into the culture water of initial auricularia and juvenile sea cucumber, the feeding of initial auricularia can be increased, the water quality can be effectively improved, the culture density can be increased, the normal growth of initial auricularia can be effectively avoided by frequent water change, the survival rate of initial auricularia and juvenile sea cucumber is improved, and the technical problem of high difficulty and low efficiency of breeding seedlings of Apostichopus japonicus is effectively solved.

Description

TECHNICAL FIELD

[0001] The present application belongs to the field of sea cucumber breeding, and particularly relates to a breeding method of artificial seedling of Stichopus horrens. BACKGROUND

[0002] Stichopus horrens, scientific name: Stichopus horrens, is a marine animal of the family Stichopodidae and the genus Stichopus, and is a high-quality edible sea cucumber with thick and tender meat. Stichopus horrens has high protein content and low fat content, has high nutritional value and health care function, and is in short supply on the international and domestic markets, and has extremely high economic benefits. However, the current artificial breeding technology of Stichopus horrens seedling still has many problems, which greatly restricts the expansion of the artificial breeding scale of Stichopus horrens. The main problems are as follows: 1. The initial ear larvae have high requirements for water quality and feed, have high breeding difficulty, have high mortality, and have low survival rate of seedling; 2. The juvenile sea cucumber is prone to shrinkage, skin ulceration and autolysis, and the survival rate of juvenile sea cucumber is low. SUMMARY

[0003] The present application aims to overcome the deficiencies in the prior art, and provides a breeding method of artificial seedling of Stichopus horrens, which can effectively increase the egg production of parent sea cucumber, improve the survival rate of initial ear larvae and juvenile sea cucumber, and is beneficial to increasing the market supply of sea cucumber seedlings.

[0004] The present application is implemented as follows:

[0005] A breeding method of artificial seedling of Stichopus horrens comprises the following steps:

[0006] (1) The selected parent sea cucumber is placed in a parent sea cucumber breeding tank, and is fed with bait composed of Sargassum thunbergii powder, Sargassum siliquastrum powder, sea mud, chitosan, fucoidan, lecithin, vitamin E, vitamin A, and pineapple enzyme;

[0007] (2) In the egg-laying season of Stichopus horrens from May to September, the induction of egg laying is performed within 3 days before and after the first day of the lunar calendar every month, bait feeding is stopped 2 days before the induction of egg laying, in the afternoon of the day of the induction of egg laying, healthy parent sea cucumber with full abdomen is selected, the surface of the sea cucumber is washed clean with filtered seawater, and then the sea cucumber is placed in an indoor induction of egg laying tank to dry for 40-60 min, then the sea cucumber is stimulated with flowing filtered seawater for 30-40 min, and then the sea cucumber is re-injected with seawater, and the spermatozoa are discharged;

[0008] (3) After the parent sea cucumber discharges the spermatozoa, the parent sea cucumber is taken out, and the fertilized eggs are hatched in the original induction of egg laying tank;

[0009] (4) after hatching out the initial ear larvae, collect the initial ear larvae and put them into the breeding tank for cultivation, wait for the initial ear larvae to gradually develop into the drum-shaped larvae, juvenile sea cucumbers, and until the cultivation into young sea cucumbers; during the cultivation process, fecal enterococcus, bacillus licheniformis, lactobacillus, vibrio and butyric acid clostridium are put into the water body, and are put in once a day; the put-in amount is, in terms of the water body volume, 0.05-0.10 mg / L of fecal enterococcus, 0.05-0.10 mg / L of bacillus licheniformis, 0.05-0.10 mg / L of lactobacillus, 0.1-0.5 mg / L of vibrio and 0.1-0.5 mg / L of butyric acid clostridium; on the first to fifth day of the young sea cucumber breeding, 5-7% of the sand-filtered seawater of the breeding water body is added every day, and after the sixth day, 10-15% of the water is changed every day; the young sea cucumber is cultivated and developed into the drum-shaped larvae after 10-14 days.

[0010] Further, in the step (1), the bait for the parent sea cucumber is put in once a day in the evening, and the bait amount is 2-3% of the body weight of the parent sea cucumber; if the parent sea cucumber cultivation tank is an indoor cement tank, continuous water flow and aeration are maintained, and the residual bait and feces at the bottom of the tank are regularly removed; if the parent sea cucumber cultivation tank is an outdoor pond, the water is changed once every other day.

[0011] Preferably, the step (1) is: selecting natural rough sea cucumbers with a body weight of 400-850 g, and breeding them in an indoor cement tank or an outdoor pond, with a breeding density of 5-8 heads / m 2 in the indoor cement tank and 1-2 heads / m 2 in the outdoor pond, and placing hiding places at the bottom of the tank; feeding the parent sea cucumber once a day in the evening, with a daily feeding amount of 2-3% of the body weight of the parent sea cucumber, wherein the feeding bait for the parent sea cucumber includes, in terms of weight fraction, 10-20 parts of sargassum thunbergii, 10-20 parts of sargassum siliquosum, 25-35 parts of sea cucumber parent sea cucumber compound feed, 5-10 parts of sea mud, 0.1-1 part of chitosan, 0.1-1 part of fucoidan, 0.1-1 part of lecithin, 0.1-0.5 part of vitamin E, 0.1-0.5 part of vitamin A and 0.1-0.3 part of pineapple enzyme; the daily water flow of the indoor cement tank is 2-4 times the cultivation water body; the water change amount of the outdoor pond is 10-20% of the water body of the pond each time; the salinity of the indoor cement tank and the outdoor pond needs to be maintained at 26-33, and the dissolved oxygen content needs to be more than 5 mg / L.

[0012] Further preferably, in the step (1), the feeding bait for the parent sea cucumber includes, in terms of weight fraction, 15 parts of sargassum thunbergii, 15 parts of sargassum siliquosum, 30 parts of sea cucumber parent sea cucumber compound feed, 8 parts of sea mud, 0.5 part of chitosan, 0.5 part of fucoidan, 0.5 part of lecithin, 0.3 part of vitamin E, 0.3 part of vitamin A and 0.2 part of pineapple enzyme.

[0013] Further, in the step (3), after the spawning of the parent sea cucumber, a small mesh net made of nylon thread is used to catch the parent sea cucumber, and a 300-mesh screen silk net box is used for siphon water exchange, with 20-30% water exchange volume each time.

[0014] Further, in the step (4), after the fertilized eggs are hatched and developed into initial ear larvae, the bait of M. mucronata, chlorella, and spirulina powder is used, with 2 times of bait feeding per day.

[0015] Preferably, after the fertilized eggs are hatched and developed into initial ear larvae, the initial ear larvae are collected by siphon method, and then placed in a breeding tank for cultivation, with a stocking density of 0.1-0.5 individuals / mL; the bait of M. mucronata, chlorella, and spirulina powder is used, with 2 times of bait feeding per day, with 10-20 thousand cells / mL of M. mucronata, 1000-2000 cells / mL of chlorella, and 0.02-0.12 mg / L of spirulina powder.

[0016] Further, in the step (4), when 10-20% of the initial ear larvae metamorphose into barrel-shaped larvae, corrugated plates are used for seedling collection, with 130-150 pieces of corrugated plates of 40 cm x 30 cm specifications per square meter; after the corrugated plates are placed, 2 times of bait feeding per day is performed, with 20 thousand cells / mL of M. mucronata, 2000 cells / mL of chlorella, and 0.12 mg / L of spirulina powder each time; during the seedling collection, water exchange is stopped, and after the barrel-shaped larvae are completely attached, water flow is started to improve water quality.

[0017] Further, in the step (4), within 10 days after the barrel-shaped larvae are attached and metamorphosed into juvenile sea cucumbers, in addition to the bait of chlorella and spirulina powder, juvenile sea cucumber compound feed, sargassum thunbergii powder, sargassum thunbergii powder, and sea mud are started to be fed, with 1 feeding each morning and evening under the conditions of micro-flow and continuous aeration; from the 11th to the 40th day after the juvenile sea cucumbers are attached, the feeding of spirulina powder is stopped, and the rest of the bait is fed 1 time each morning and evening; the cultivation is carried out under the conditions of micro-flow and continuous aeration; the bottom residual bait and feces are cleaned up once every 5-7 days, the juvenile sea cucumbers are collected using a 40-mesh screen silk net box, and the collected juvenile sea cucumbers are moved to the breeding tank in time; when the juvenile sea cucumbers grow to a body length of 1.0 cm, the cultivation of young sea cucumbers is started.

[0018] Further, in the step (4), during the cultivation of young sea cucumbers, the bait of young sea cucumber compound feed, sargassum thunbergii powder, sargassum thunbergii powder, and sea mud is used, with 1 feeding each morning and evening under the conditions of micro-flow and continuous aeration; the bottom residual bait and feces are cleaned up once every 3-5 days, the young sea cucumbers are collected using a 20-mesh screen silk net box, and the cultivation is continued for 40 days; the specific operation is as follows: 40 days after the barrel-shaped larvae are attached and metamorphosed into juvenile sea cucumbers, the body length of the sea cucumber seedlings grows to more than 1 cm, the feeding concentration is 8-10 g / m 2 of young sea cucumber compound feed, 8-10 g / m2 Sargassum powder 6-8 g / m 2 Sea mud 8-10.0 g / m 2 Each morning and evening, 1 time; under the conditions of micro-flow water and continuous aeration, the daily flow water amount is 1.0-1.5 times of the water body for breeding, the dissolved oxygen is kept above 5 mg / L; the residual feed and feces at the bottom of the pool are removed once every 3-5 days; when the pool bottom is flushed, the young sea cucumbers flushed out are collected by a 20-mesh silk net box and then moved back to the breeding pool in time to avoid the young sea cucumbers from being damaged and dying.

[0019] The present application greatly improves the spawning quantity of the parent sea cucumber by improving the structure of the parent sea cucumber feed; the Enterococcus faecalis, Bacillus licheniformis, Lactobacillus, Vibrio and Clostridium butyricum are put into the breeding water body of the initial ear larvae and juvenile sea cucumbers, so that the feeding of the initial ear larvae is increased, the water quality is effectively improved, the breeding density is improved, and the normal growth of the initial ear larvae is effectively avoided due to frequent water change, and the survival rate of the initial ear larvae and juvenile sea cucumbers is improved. The successful rate of the parent sea cucumber induced spawning is 95%, the cultivation rate of the initial ear larvae to seedlings is 4.92%, and about 16,000 seedlings with a length of more than 2 cm are cultivated in one square meter of indoor breeding pool, which effectively solves the technical problems of high difficulty and low efficiency of sea cucumber breeding. DETAILED DESCRIPTION

[0020] The technical solutions of the present application will be clearly and completely described below in combination with the embodiments of the present application. Obviously, the described embodiments are only part of the embodiments of the present application, rather than all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative labor fall within the scope of protection of the present application.

[0021] Embodiment 1

[0022] Embodiment 1

[0023] A method for artificial seedling breeding of Acaudina molpadioidea, comprising the following steps:

[0024] (1) Selecting natural Acaudina molpadioidea with a weight of 400-850 g and releasing them into a parent sea cucumber breeding pool; the parent sea cucumber breeding pool can be an indoor cement pool or an outdoor pond; the releasing density in the indoor water pool is 5-8 heads / m 2 , and the releasing density in the outdoor pond is 1-2 heads / m 2, and the bottom of the pool is placed with a hidden object; the bait is fed once a day in the evening, and the daily feeding amount is 2-3% of the weight of the parent sea cucumber; according to weight, the feeding bait of the parent sea cucumber comprises 10-20 parts of sargassum thunbergii, 10-20 parts of sargassum siliquosum, 25-35 parts of sea cucumber parent sea cucumber compound feed, 5-10 parts of sea mud, 0.1-1 part of chitosan, 0.1-1 part of fucoidan, 0.1-1 part of lecithin, 0.1-0.5 part of vitamin E, 0.1-0.5 part of vitamin A, and 0.1-0.3 part of pineapple enzyme; the indoor cement pool is continuously kept flowing and aerated, and the daily water flow is 2-4 times of the water body in the pool; the bottom of the pool is regularly cleaned of residual bait and feces; the outdoor pond is changed once a day, and the daily water change amount is 10-20% of the water body in the pond; the salinity of seawater is 26-33, and the dissolved oxygen content is more than 5 mg / L;

[0025] (2) In the spawning season of the rough sea cucumber from May to September, the parent sea cucumber is induced to spawn within 3 days before and after the first day of the lunar calendar every month, the bait is stopped 2 days before artificial stimulation of oocyte collection, and the parent sea cucumber is selected for stimulation of oocyte collection in the afternoon of the day, the parent sea cucumber is washed clean with filtered seawater, then is placed in an indoor stimulation oocyte collection pool for 40-60 min of air drying, then is stimulated with filtered seawater for 30-40 min, and then is re-injected with seawater until the sperm and egg are discharged;

[0026] (3) After the parent sea cucumber discharges the sperm and egg, the parent sea cucumber is hatched in the original stimulation oocyte collection pool, and the parent sea cucumber is fished out with a small net made of nylon thread, and is siphoned with a 300-mesh gauze net box, and the water is changed 1-2 times, and the water change amount is 20-30% each time;

[0027] (4) After the initial auricularia larvae are hatched, the initial auricularia larvae are collected and placed in a breeding pool for cultivation, and the initial auricularia larvae are gradually developed into a drum-shaped larva, a juvenile sea cucumber, and finally a young sea cucumber; during the cultivation process, fecal enterococcus, bacillus licheniformis, and lactobacillus are added to the water body, and are added once a day; the addition amount is 0.05-0.10 mg / L of fecal enterococcus, 0.05-0.10 mg / L of bacillus licheniformis, 0.05-0.10 mg / L of lactobacillus, 0.1-0.5 mg / L of vibrio, and 0.1-0.5 mg / L of clostridium butyricum.

[0028] After the fertilized eggs are hatched into the initial ear larvae, the initial ear larvae are collected by using the siphon method, and then the initial ear larvae are placed in a rearing pond for cultivation. The initial ear larvae are placed at a density of 0.1-0.5 individuals / mL. The feed is composed of Chaetoceros muelleri, Isochrysis galbana and spirulina powder. The feed is fed twice a day. Chaetoceros muelleri is fed at a concentration of 10-20 thousand cells / mL, Isochrysis galbana is fed at a concentration of 1-2 thousand cells / mL, and spirulina powder is fed at a concentration of 0.02-0.12 mg / L. From the first day to the fifth day, 5-7% of the sand-filtered seawater is added to the water body of the rearing pond every day. From the sixth day, 10-15% of the water is changed every day. The larvae are cultivated for 10-14 days to develop into the urn-shaped larvae.

[0029] When 10-20% of the initial ear larvae develop into the urn-shaped larvae, the wave-shaped plates are placed for the seedling collection. Each square meter is provided with 130-150 pieces of wave-shaped plates with a size of 40 cm x 30 cm. After the wave-shaped plates are placed, the urn-shaped larvae are attached. During this period, the feed is fed twice a day. Chaetoceros muelleri is fed at a concentration of 20 thousand cells / mL, Isochrysis galbana is fed at a concentration of 2 thousand cells / mL, and spirulina powder is fed at a concentration of 0.12 mg / L. During the seedling collection, the water is not changed. After the urn-shaped larvae are completely attached, the water is started to be changed to improve the water quality.

[0030] Within 10 days after the urn-shaped larvae are attached and metamorphosed into the juvenile sea cucumbers, in addition to the feeding of Isochrysis galbana and spirulina powder, the juvenile sea cucumber compound feed, Sargassum thunbergii powder, Sargassum siliquastrum powder and sea mud are started to be fed. The feed is fed once in the morning and once in the evening. The cultivation is carried out under the conditions of micro-flow water and continuous aeration. From the eleventh day to the fortieth day after the juvenile sea cucumbers are attached, the feeding of spirulina powder is stopped. The rest of the feed is fed once in the morning and once in the evening. The cultivation is carried out under the conditions of micro-flow water and continuous aeration. The bottom residual feed and feces are cleaned once every 5-7 days. The juvenile sea cucumbers are collected by using a 40-mesh silk net box, and the collected juvenile sea cucumbers are moved to the rearing pond in time. When the juvenile sea cucumbers grow to a body length of 1.0 cm, the juvenile sea cucumber cultivation stage is started.

[0031] In the juvenile sea cucumber cultivation stage, the juvenile sea cucumber compound feed, Sargassum thunbergii powder, Sargassum siliquastrum powder and sea mud are used as the feed, which is fed once in the morning and once in the evening. The cultivation is carried out under the conditions of micro-flow water and continuous aeration. The bottom residual feed and feces are cleaned once every 3-5 days. The juvenile sea cucumbers are collected by using a 20-mesh silk net box. The cultivation is carried out for 40 days. The specific operation is as follows: 40 days after the urn-shaped larvae are attached and metamorphosed into the juvenile sea cucumbers, the body length of the seedlings reaches 1 cm or more. The concentration of the feed is 8-10 g / m 2 for the juvenile sea cucumber compound feed, 8-10 g / m 2 for Sargassum thunbergii powder, 6-8 g / m 2 for Sargassum siliquastrum powder, and 8-10.0 g / m 2; each morning and evening 1 times; under the conditions of micro-flow and continuous aeration, the daily flow water is 1.0-1.5 times of the water body for seedling raising, and the dissolved oxygen is maintained above 5 mg / L; the residual bait and feces at the bottom of the pool are removed once every 3-5 days; when the bottom of the pool is flushed, the young sea cucumbers flushed out are collected in a 20-mesh silk net box and then moved back to the seedling raising pool in time to avoid the accumulation of the young sea cucumbers and the injury and death of the young sea cucumbers.

[0032] The success rate of oviposition induced by the parent sea cucumber according to the application is 95%, the seedling raising rate of the initial ear larvae is 4.92%, and about 16000 seedlings with a length of more than 2 cm are raised per square meter of indoor seedling raising pool, which solves the technical problem of high difficulty in seedling raising of the sea cucumber and greatly improves the breeding efficiency of the artificial seedlings of the sea cucumber.

[0033] Effects of different parent sea cucumber breeding methods on oviposition of parent sea cucumber

[0034] Forty parent sea cucumbers selected at random are randomly divided into four groups. The parent sea cucumbers in the control group are bred according to the method in the application no. 201510127244.0 entitled “A breeding method of artificial seedlings of sea cucumber”. The feed composition of the experimental group 1 is as follows: Sargassum thunbergii powder 10-20 parts, Sargassum siliquastrum powder 10-20 parts, sea cucumber parent sea cucumber compound feed 25-35 parts, sea mud 5-10 parts, vitamin E 0.1-0.5 parts, vitamin A 0.1-0.5 parts, and pineapple enzyme 0.1-0.3 parts. The feed composition of the experimental group 2 is as follows: Sargassum thunbergii powder 10-20 parts, Sargassum siliquastrum powder 10-20 parts, sea cucumber parent sea cucumber compound feed 25-35 parts, sea mud 5-10 parts, chitosan 0.1-1 part, brown algal polysaccharide 0.1-1 part, lecithin 0.1-1 part, vitamin E 0.1-0.5 part, and vitamin A 0.1-0.5 part. The feed composition of the experimental group 3 is as follows: Sargassum thunbergii powder 10-20 parts, Sargassum siliquastrum powder 10-20 parts, sea cucumber parent sea cucumber compound feed 25-35 parts, sea mud 5-10 parts, chitosan 0.1-1 part, brown algal polysaccharide 0.1-1 part, lecithin 0.1-1 part, vitamin E 0.1-0.5 part, vitamin A 0.1-0.5 part, and pineapple enzyme 0.1-0.3 part. The experimental groups 1-3 are induced for oviposition and hatching according to the method in example 1. The data of oviposition of the parent sea cucumbers are counted and shown in Table 1.

[0035] Table 1 Comparison of oviposition effects of different parent sea cucumber breeding methods

[0036] Parameter Objective (head) Induced oviposition success rate (%) Total oviposition amount (thousands of eggs) Hatching rate (%) 20 85 3360 73.8 Control group 20 75 3200 85.7 Experimental group 1 20 90 3600 95.4 Experimental group 2 Experimental group 3 20 95 4350 96.5

[0037] According to the data in Table 1, the success rate of oviposition induced by the parent sea cucumber according to the application can reach 95%, and the total oviposition amount of 20 parent sea cucumbers is more than 40 million, which is significantly improved compared with the control group and the experimental groups 1 and 2. Meanwhile, the hatching rate of the fertilized eggs in the example 1 group of the application can reach 96.5%, which is much higher than 73.8% of the control group.

[0038] Effects of different cultivation methods on the growth of juveniles

[0039] The juveniles are hatched according to the parent participation cultivation method and the fertilized egg hatching method described in the present application, and are released at 0.5 individuals / mL into different rearing ponds, and are cultivated by different methods. Among them, the control group is cultivated according to the cultivation method in the application number 201510127244.0, entitled "A cultivation method of artificial seedlings of Apostichopus japonicus", the experimental group 1 refers to the culture method described in the present application example 1, the experimental group 2 refers to the culture method of the present application example 1, but Enterococcus faecalis, Bacillus licheniformis and Lactobacillus are put into the water, the experimental group 3 refers to the culture method of the present application example 1, but Enterococcus faecalis, Bacillus licheniformis, Lactobacillus and Vibrio are put into the water, and the experimental group 4 refers to the culture method of the present application example 1, but Enterococcus faecalis, Bacillus licheniformis, Lactobacillus and Clostridium butyricum are put into the water. The number of seedlings above 2 cm is counted, and the effects of different cultivation methods on the growth of juveniles are compared, as shown in Table 2.

[0040] Table 2 Comparison of effects of different cultivation methods

[0041]

[0042]

[0043] According to the data in Table 2, the culture method described in the present application example 1 can significantly improve the seedling rate of juveniles, and the seedling rate can reach 4.92%, which is significantly higher than that of the control group and the experimental groups 2-4. The number of seedlings per square meter can reach 16-19 thousand in the present application example 1, while the highest number of seedlings per square meter in the other treatment groups is 12-13 thousand. It shows that the cultivation method described in the present application can significantly improve the seedling rate of juveniles, and the number of seedlings per square meter is also significantly improved, the success rate and efficiency of the cultivation of Apostichopus japonicus seedlings are greatly improved, which provides a new way for large-scale cultivation of Apostichopus japonicus seedlings.

[0044] The above only describes the preferred embodiments of the present application, and is not intended to limit the present application. Any modification, equivalent replacement, improvement, etc. made within the spirit and principles of the present application shall be included in the protection scope of the present application.

Claims

1. A method for artificial seedling breeding of Apostichopus japonicus, characterized in that, It comprises the following steps: (1) Put the selected parent into the parent culture pool, feed the bait composed of sargassum thunbergii powder, sargassumvateroides powder, sea mud, chitosan, fucoidan, lecithin, vitamin E, vitamin A and pineapple enzyme; (2) In the spawning season of the rough sea cucumber from May to September, the spawning is induced within 3 days before and after the first day of the lunar calendar every month, the bait is stopped 2 days before the artificial stimulation of oocyte collection, the healthy parent with full abdomen is selected in the afternoon of the day of stimulation of oocyte collection, the surface of the parent is washed clean with filtered seawater, then it is placed in the indoor stimulation oocyte collection pool to dry for 40-60 min, then it is stimulated with water flow for 30-40 min, and then the seawater is re-injected until the spermatozoa are discharged; (3) After the parent discharges the spermatozoa, the parent is taken out, and the zygotes are hatched in the original stimulation oocyte collection pool; (4) After the initial auricularia larvae are hatched, the initial auricularia larvae are collected and placed in the larval rearing pool for cultivation, and the initial auricularia larvae are gradually developed into ampullae larvae, juvenile sea cucumbers and finally young sea cucumbers; during the cultivation process, enterococcus faecalis, bacillus licheniformis, lactobacillus, vulvovaginalis and butyric acid clostridium are added to the water body once a day; the adding amount is 0.05-0.10 mg / L of enterococcus faecalis, 0.05-0.10 mg / L of bacillus licheniformis, 0.05-0.10 mg / L of lactobacillus, 0.1-0.5 mg / L of vulvovaginalis and 0.1-0.5 mg / L of butyric acid clostridium, calculated based on the volume of the water body; 5-7% of sand-filtered seawater is added to the larval rearing pool every day from the first day to the fifth day, and the water is changed by 10-15% every day after the sixth day; the larvae are cultivated and developed into ampullae larvae after 10-14 days; The step (1) selects natural Stichopus horrens with a weight of 400-850 g, and the Stichopus horrens are cultured in indoor cement pools or outdoor ponds. The indoor water pool has a stocking density of 5-8 individuals / m 2 , the outdoor pond has a stocking density of 1-2 individuals / m 2 , and the pool bottom is provided with shelters. The Stichopus horrens are fed once a day in the evening, and the daily feeding amount is 2-3% of the weight of the Stichopus horrens. According to weight, the feeding bait of the Stichopus horrens includes 10-20 parts of Sargassum thunbergii, 10-20 parts of Sargassum siliquastrum, 25-35 parts of Stichopus horrens parent Stichopus horrens compound feed, 5-10 parts of sea mud, 0.1-1 part of chitosan, 0.1-1 part of fucoidan, 0.1-1 part of lecithin, 0.1-0.5 part of vitamin E, 0.1-0.5 part of vitamin A, and 0.1-0.3 part of pineapple enzyme. The indoor cement pool has a daily water flow of 2-4 times the culture water body. The outdoor pond has a water change amount of 10-20% of the pond water body each time. The indoor cement pool and the outdoor pond both need to maintain a seawater salinity of 26-33 and a dissolved oxygen content of more than 5 mg / L.

2. The method for artificial seedling propagation of sea cucumber as described in claim 1, characterized in that, In step (1), the bait for the parent is added once a day in the evening, and the bait amount is 2-3% of the weight of the parent; if the parent culture pool is an indoor cement pool, the water is kept flowing and aerated continuously, and the residual bait and feces at the bottom of the pool are cleaned regularly; if the parent culture pool is an outdoor pond, the water is changed once every other day.

3. The method for artificial seed propagation of the sea cucumber Stichopus horrens as claimed in claim 1, wherein, In step (1), the bait for the parent comprises, by weight fraction, 15 parts of sargassum thunbergii powder, 15 parts of sargassumvateroides powder, 30 parts of sea cucumber parent compound feed, 8 parts of sea mud, 0.5 parts of chitosan, 0.5 parts of fucoidan, 0.5 parts of lecithin, 0.3 parts of vitamin E, 0.3 parts of vitamin A and 0.2 parts of pineapple enzyme.

4. The method for artificial seed propagation of the sea cucumber Stichopus horrens as claimed in claim 1, wherein, In step (3), after the parent discharges the spermatozoa, the parent is taken out with a small net made of nylon thread, and the water is changed with a 300-mesh gauze net box siphon, 1-2 times, with a water change amount of 20-30% each time.

5. The method for artificial seed propagation of the sea cucumber Stichopus horrens as claimed in claim 1, wherein, In step (4), after the zygotes are hatched into initial auricularia larvae, muggiaea gaudichaudii, chrysochromulina globifera and spirulina powder are used as bait, and the bait is added twice a day.

6. The method for artificial seed propagation of the sea cucumber Stichopus horrens as claimed in claim 5, wherein, After the fertilized eggs are hatched into initial ear larvae, the initial ear larvae are collected by using a siphon method, and then the initial ear larvae are placed in a rearing pond for cultivation, the initial ear larvae are placed at a density of 0.1-0.5 pieces / mL; the Mugilar chuii, Isochrysis galbana and spirulina powder are used as baits, the Mugilar chuii is 10-20 thousand cells / mL, the Isochrysis galbana is 1000-2000 cells / mL, and the spirulina powder is 0.02-0.12 mg / L, and the baits are fed twice a day.

7. The method for artificial seedling propagation of sea cucumber as described in claim 1, characterized in that, In the step (4), when 10-20% of the initial ear larvae metamorphose into the bottle-shaped larvae, the corrugated plates are put for seedling collection, 130-150 pieces of the corrugated plates with a specification of 40 cm*30 cm are put per square meter; after the corrugated plates are put, the bottle-shaped larvae are attached, and the baits are fed twice a day, the Mugilar chuii is 20 thousand cells / mL, the Isochrysis galbana is 2000 cells / mL, and the spirulina powder is 0.12 mg / L; during the seedling collection, the water is not changed, and after the bottle-shaped larvae are attached, the water is changed to improve the water quality.

8. The method for artificial seed propagation of the sea cucumber Stichopus horrens as claimed in claim 1, wherein the sea cucumber Stichopus horrens is Stichopus horrens of the species Stichopus horrens. In the step (4), within 10 days after the bottle-shaped larvae are attached and metamorphosed into the juvenile sea cucumbers, in addition to the Isochrysis galbana and the spirulina powder, the juvenile sea cucumber compound feed, the Sargassum thunbergii powder, the Sargassum vachellianum powder and the sea mud are started to be fed, and the baits are fed once in the morning and once in the evening, and the juvenile sea cucumbers are cultivated under the conditions of micro-flow water and continuous aeration; on the 11th day to the 40th day after the juvenile sea cucumbers are attached, the spirulina powder is stopped to be fed, and the rest of the baits are fed once in the morning and once in the evening; the juvenile sea cucumbers are cultivated under the conditions of micro-flow water and continuous aeration; the residual baits and feces on the bottom of the pond are cleaned once every 5-7 days, the 40-mesh silk net box is used to collect the juvenile sea cucumbers, and the collected juvenile sea cucumbers are moved to the rearing pond in time; when the juvenile sea cucumbers grow to a body length of 1.0 cm, the juvenile sea cucumbers enter the young sea cucumber cultivation stage. ​ 9. The method for artificial seedling propagation of sea cucumber as described in claim 1, characterized in that, The step (4) is carried out as follows: the young sea cucumber is fed with the young sea cucumber compound feed, the sargassum thunbergii powder, the sargassum funtus powder and the sea mud, and the feeding is carried out once in the morning and once in the evening; the young sea cucumber is bred under the conditions of micro-flow water and continuous aeration; the residual bait and feces at the bottom of the pool are removed once every 3-5 days; the young sea cucumber is collected by using a 20-mesh gauze box, and the breeding is continued for 40 days; the specific operation is as follows: 40 days after the attachment metamorphosis of the barrel-shaped young sea cucumber into the juvenile sea cucumber, the sea cucumber seedling grows to be more than 1 cm in length; the feeding concentration is 8-10 g / m 2 of the young sea cucumber compound feed, 8-10 g / m 2 of the sargassum thunbergii powder, 6-8 g / m 2 of the sargassum funtus powder and 8-10.0 g / m 2 of the sea mud; the feeding is carried out once in the morning and once in the evening; the young sea cucumber is bred under the conditions of micro-flow water and continuous aeration, the daily flow water is 1.0-1.5 times the water body for breeding, the dissolved oxygen is maintained to be more than 5 mg / L; the residual bait and feces at the bottom of the pool are removed once every 3-5 days; when the bottom of the pool is flushed, the young sea cucumber flushed out is collected by using a 20-mesh gauze box, and is timely moved back to the breeding pool, so as to avoid the accumulation, injury and death of the young sea cucumber.

Images