Functional suspended crystal ball and its manufacturing method

Abstract

The present invention proposes a functional suspension modeling crystal ball, which includes: a main structure and an active ingredient, the active ingredient is filled in the main structure or attached to the outside of the main structure. In addition, the present invention proposes a method for manufacturing a functional suspension modeling crystal ball, which includes: providing a mold, the mold has a molding cavity; adding a crystal ball composition liquid into the molding cavity of the mold; and forming a functional suspension modeling crystal ball with the crystal ball composition liquid in the molding cavity through the molding composition liquid, and the functional suspension modeling crystal ball includes a main structure and an active ingredient, the active ingredient is filled in the main structure or attached to the outside of the main structure. The functional suspension modeling crystal ball of the present invention has the advantage of protecting the active ingredient to prevent the active ingredient from contacting with the environment and causing deterioration.

Description

Technical Field

[0001] The invention relates to a suspended crystal ball and a manufacturing method thereof, and in particular to a functional suspended modeling crystal ball and a manufacturing method thereof. Background Art

[0002] In order to allow consumers to see the presence of active ingredients with their naked eyes or as a sales gimmick, cosmetics manufacturers usually make active ingredients into visible dosage forms, such as crystal balls, water-in-oil forms, or oil-in-water forms. However, the existing production methods mostly use microfluidic equipment, which leads to high production costs.

[0003] For example, the "collagen liposphere structure with the function of promoting skin firmness, whitening and translucent skin and increasing skin elasticity" proposed in Taiwan Utility Model Patent Publication No. M466662 uses microfluidic and thin film pressurized injection technology to manufacture Lipogen collagen lipospheres. Microfluidic technology is to pass through an oily solvent containing lipids, use water to squeeze out the oil droplets near the outlet, form a small protrusion at the outlet of the flow channel, and cut off the vacuole at the right time, which can continuously produce collagen lipospheres of uniform size; thin film pressurized injection technology uses a filter membrane with appropriate pore size to obtain the best pressurized filtration pressure and flow rate to manufacture collagen lipospheres of uniform size.

[0004] For another example, in the "structural color material and cosmetics using the same" proposed in Taiwan Invention Patent Publication No. 201400556, the structural color material is composed of resin particles with structural colors. The resin particles are injected into a microtubular flow channel at a certain flow rate into a fluid mixed with an aqueous medium containing a water-soluble free radical polymerization initiator and a free radical polymerizable monomer. After the fluid temperature instantly reaches a preset temperature, a portion of the free radical polymerizable monomer is reacted in the presence of a water-soluble free radical polymerization initiator, so that uniform particle cores of the resin particles are formed in the microtubular flow channel, and then a polymerization reaction liquid containing the obtained particle core is subjected to a polymerization reaction in a reactor equipped with a stirring device to obtain resin particles. The average particle size of the resin particles is in the range of 150 to 400 nm, and the absolute value of the zeta potential is in the range of 30 to 80 mV.

[0005] For another example, the "device for manufacturing particles of equal particle size" proposed in Taiwan Utility Model Patent Publication No. M421160 includes a base with a space, the base is respectively provided with a first flow channel and a second flow channel connected to the space, and the base is provided with an output flow channel that collects the first flow channel and the second flow channel, the output flow channel is for flowing into the space, and the output flow channel is provided with an output port; a driving member combined with the base, the driving member is provided with a push rod that slides in the space, and the push rod is provided with through holes corresponding to the first flow channel and the second flow channel; the driving member drives the push rod to slide laterally in the space, so that the through hole of the push rod is sequentially and cyclically aligned with the first flow channel and the second flow channel, so that the raw materials can be used to manufacture particles of equal particle size after being separated by gas, and used in skin care products.

[0006] Therefore, it is indeed one of the issues that people in the art of visible dosage form cosmetics are actively trying to solve in order to improve the problems existing in the prior art. Summary of the invention

[0007] An object of the present invention is to provide a suspension crystal sphere which can protect active ingredients to prevent the active ingredients from being deteriorated due to contact with the environment.

[0008] Another object of the present invention is to provide a suspended crystal ball that can be suspended at the interface between two liquid layers so that consumers can observe the active ingredients with their naked eyes.

[0009] Another object of the present invention is to provide a suspended crystal ball, which can be mixed with two liquids and quickly form an interface between the two liquid layers as the liquids are layered, so as to reduce the contact time with the environment.

[0010] Another object of the present invention is to provide a suspended crystal ball that can be prepared by using a microfluidic device or other non-microfluidic device methods, thereby reducing the production cost.

[0011] Therefore, the present invention proposes a functional suspended crystal ball, which includes: a main structure and an active component, wherein the active component is filled in the main structure or attached to the outside of the main structure.

[0012] Preferably, the specific gravity of the functional suspension modeling crystal ball is 0.95 to 1.

[0013] Preferably, the main structure contains sodium hyaluronate, polyacrylic acid, sodium alginate, xanthan gum, sclerotium gum, hydroxyethyl cellulose, and guar gum.

[0014] Preferably, the active ingredient is a plant extract.

[0015] According to the present invention, the functional suspension crystal ball is suspended at the interface between two liquid layers in a normal state. When in use, the functional suspension crystal ball can be shaken to mix the two liquid layers, and then the functional suspension crystal ball can be taken out; thereafter, the functional suspension crystal ball and the two liquids are allowed to stand to separate the liquids and form an interface between the two liquid layers, thereby reducing the contact time with the environment, thereby preventing the main structure or active ingredients from deteriorating. In addition, by filling the main structure with active ingredients or attaching them to the outside of the main structure, the active ingredients can be protected to prevent them from contacting the environment and deteriorating.

[0016] On the other hand, the present invention proposes a method for manufacturing a functional suspended crystal ball, which includes: providing a mold, the mold having a molding cavity; adding a crystal ball composition liquid into the molding cavity of the mold; and forming a functional suspended crystal ball through the molding composition liquid. The functional suspended crystal ball includes a main structure and an active ingredient, and the active ingredient is filled in the main structure or attached to the outside of the main structure.

[0017] Preferably, the crystal ball composition liquid contains a solvent, sodium hyaluronate, polyacrylic acid, sodium alginate, tris-gum, sclerotium gum, hydroxyethyl cellulose, guar gum, and an active ingredient.

[0018] Preferably, based on the total weight of the crystal ball composition liquid, the content of sodium hyaluronate is 0.01 to 5wt%, the content of polyacrylic acid is 0.5 to 5wt%, the content of sodium alginate is 0.5 to 5wt%, the content of Sanxian gum is 0.5 to 5wt%, the content of Sclerotium gum is 0.5 to 2wt%, the content of hydroxyethyl cellulose is 0.04 to 2wt%, the content of guar gum is 0.04 to 2wt%, the content of active ingredients is 0.05 to 5wt%, and the content of solvent is supplemented to 100wt%.

[0019] Preferably, the pH value of the crystal ball composition liquid is pH 4 to 8.

[0020] Preferably, the molding composition liquid contains a solvent, calcium carbonate, calcium lactate, calcium chloride, barium chloride, and potassium chloride.

[0021] Preferably, based on the total weight of the molding composition, the content of calcium carbonate is 0.5 to 2 wt%, the content of calcium lactate is 0.5 to 2 wt%, the content of calcium chloride is 0.5 to 2 wt%, the content of barium chloride is 0.5 to 2 wt%, the content of potassium chloride is 0.5 to 2 wt%, and the content of the solvent is supplemented to 100 wt%.

[0022] Preferably, the pH value of the molding composition liquid is pH 4 to 8.

[0023] Preferably, before adding the crystal ball composition liquid into the forming cavity, the forming cavity of the mold may be wetted with the forming composition liquid.

[0024] In another aspect, the present invention provides a composition comprising: a first liquid layer, a second liquid layer, and the functional suspended crystal sphere as described above, wherein an interface is formed between the first liquid layer and the second liquid layer, and the functional suspended crystal sphere is suspended at the interface.

[0025] Preferably, the first liquid layer contains a solvent, glycerin, an active ingredient, and alcohol.

[0026] Preferably, the second liquid layer contains cyclopentasiloxane, polydimethylsiloxane, phenylpropyldimethylsiloxysilicate, and cetearyl methicone.

[0027] Preferably, based on the total weight of the first liquid layer, the content of glycerol is 5 to 20 wt %, the content of active ingredient is 0.1 to 20 wt %, the content of alcohol is 1 to 10 wt %, and the content of solvent is supplemented to 100 wt %.

[0028] Preferably, based on the total weight of the second liquid layer, the content of cyclopentasiloxane is 5 to 40 wt %, the content of polydimethylsiloxane is 1 to 20 wt %, the content of phenylpropyldimethylsiloxysilicate is 1 to 20 wt %, and the content of cetearyl polymethylsiloxane is 1 to 20 wt %.

[0029] Preferably, the specific gravity of the first liquid layer is 1 to 1.5.

[0030] Preferably, the specific gravity of the second liquid layer is 0.6 to 0.95. BRIEF DESCRIPTION OF THE DRAWINGS

[0031] Figure 1 It is a schematic flow chart illustrating a manufacturing method according to one embodiment of the present invention.

[0032] Figure 2 It is a structural schematic diagram illustrating a mold used in an embodiment of the present invention.

[0033] Figure 3 This is a photograph showing a functional suspended crystal ball obtained according to an embodiment of the present invention.

[0034] Figure 4 It is a photograph showing that the functional suspended crystal ball obtained by the embodiment of the present invention is suspended at the interface between two liquid layers.

[0035] Figure 5 is a flow chart illustrating a manufacturing method according to another embodiment of the present invention;

[0036] Figure 6 is a histogram illustrating the effect of AMLE on the activity of B16F10 cells;

[0037] Fig. 7A It is a photographic diagram illustrating the protective effect of AMLW and AMLE on DNA;

[0038] Figure 7B is a histogram, illustrating the protective effect of AMLW and AMLE on DNA;

[0039] Figure 8 is a histogram, illustrating the protective effect of Allium spicatum extract on DNA;

[0040] Fig. 9 is a histogram, illustrating the effect of Allium spicatum extract on the activity of B16F10 cells;

[0041] Fig.10 is a histogram, illustrating the inhibitory effect of Allium spicatum extract on tyrosinase activity;

[0042] Fig.11 It is a photo graph, which illustrates the expression of PCNA after the action of PSB;

[0043] Fig.12 The photographs show the expression level of Lamin A protein in cells after the action of PSBL. DETAILED DESCRIPTION

[0044] In order to make the above and / or other purposes, effects, and features of the present invention more clearly understood, preferred embodiments are specifically described below in detail:

[0045] Please refer to Figure 1 One embodiment of the present invention provides a method for manufacturing a functional suspended crystal ball. Figure 3As shown, the functional suspension-shaped crystal ball prepared has a main structure and active ingredients, and the active ingredients are filled in the main structure or attached to the outside of the main structure, so as to protect the active ingredients from contact with the environment and deterioration. Figure 4 As shown, the obtained functional suspension crystal ball can be combined with the first liquid layer and the second liquid layer to form a composite and suspended at the interface formed between the first liquid layer and the second liquid layer. Based on the total weight of the first liquid layer, the first liquid layer can contain 5 to 20 wt% of glycerol, 0.1 to 20 wt% of active ingredients, 1 to 10 wt% of alcohols (such as propylene glycol, butylene glycol, or a mixture thereof), and a solvent (such as water) supplemented to 100 wt%, but not limited thereto; and the specific gravity of the first liquid layer can be 1 to 1.5, but not limited thereto. Based on the total weight of the second liquid layer, the second liquid layer may contain 5 to 40 wt% of cyclopentasiloxane, 1 to 20 wt% of polydimethylsiloxane, 1 to 20 wt% of phenylpropyldimethylsiloxysilicate, and 1 to 20 wt% of cetearyl polymethylsiloxane, but not limited thereto; and the specific gravity of the second liquid layer is 0.6 to 0.95, but not limited thereto. When in use, the composition may be shaken first to mix the functional suspension modeling crystal spheres with the two liquid layers, and then the functional suspension modeling crystal spheres may be taken out; thereafter, the functional suspension modeling crystal spheres and the two liquids may be allowed to stand to allow the two liquids to separate into layers and then form the first liquid layer and the second liquid layer, and the functional suspension modeling crystal spheres may be formed at the interface between the two liquid layers, thereby reducing the time that the functional suspension modeling crystal spheres are in contact with the environment, thereby avoiding deterioration of the main structure or active ingredients. The detailed steps of the manufacturing method of this embodiment are described as follows.

[0046] First, a mold (1) is provided, wherein the mold has a molding cavity (11) and two openings connected to the molding cavity (11) (step S11). Generally speaking, the shape of the molding cavity (11) can determine the shape of the functional suspension molding crystal ball. Figure 2 As shown, when the shape of the forming cavity (11) is heart-shaped, the shape of the functional suspension crystal ball is of course heart-shaped. It is understandable that the shape of the forming cavity (11) can also be circular, triangular, or star-shaped, but not limited thereto.

[0047] Next, one of the openings of the mold (1) is placed in the molding composition liquid (3), so that the molding composition liquid (3) wets the molding cavity (11) of the mold (1) (step S12). It should be noted that the purpose of wetting the molding cavity (11) is to facilitate the demolding of the functional suspended crystal ball obtained later from the molding cavity (11) without leaving any residue in the mold (1). Based on the total weight of the molding composition liquid (3), the molding composition liquid (3) may contain 0.5 to 2 wt% of calcium carbonate, 0.5 to 2 wt% of calcium lactate, 0.5 to 2 wt% of calcium chloride, 0.5 to 2 wt% of barium chloride, 0.5 to 2 wt% of potassium chloride, and a solvent (such as water) supplemented to 100 wt%, but not limited thereto; and the pH value of the molding composition liquid (3) may be pH 4 to 8, but not limited thereto.

[0048] Finally, the crystal ball composition liquid (2) is added into the molding cavity (11) of the mold (1), so that the crystal ball composition liquid (2) forms a functional suspended crystal ball (4) through the molding composition liquid (3) in the molding cavity (11) (steps S13, S14). Based on the total weight of the crystal ball composition liquid (2), the crystal ball composition liquid (2) may contain 0.01 to 5 wt% of sodium hyaluronate, 0.5 to 5 wt% of polyacrylic acid, 0.5 to 5 wt% of sodium alginate, 0.5 to 5 wt% of tris-gum, 0.5 to 2 wt% of sclerotium gum, 0.04 to 2 wt% of hydroxyethyl cellulose, 0.04 to 2 wt% of guar gum, 0.05 to 5 wt% of active ingredients, and a solvent (such as water) supplemented to 100 wt%, but not limited thereto; and the pH value of the crystal ball composition liquid (2) may be pH 4 to 8, but not limited thereto. In addition, the active ingredient can be defined as an ingredient having one of the effects of promoting skin protection and repair, anti-wrinkle, anti-oxidation, anti-inflammatory, anti-aging, and whitening, preferably a plant extract. For example, the plant extract can be the Cinnamomum camphora bud embryo tissue extract described in Taiwan Invention Patent Publication No. I697332, the orchid bud embryo tissue extract described in Taiwan Invention Patent Publication No. 202021609, the Curculigo extract described in Taiwan Invention Patent Publication No. 202023527, the longan flower extract described in China Invention Patent Publication No. CN109276493A, or the red quinoa extract described in China Invention Patent Publication No. CN108852924A, but not limited thereto, the full text of these documents is hereby provided for reference. It should be noted that, since the molding composition liquid (3) can polymerize the crystal ball composition liquid (2) to form a functional suspension modeling crystal ball (4) including a main structure and active ingredients, the main structure can contain sodium hyaluronate, polyacrylic acid, sodium alginate, trisaccharide gum, sclerotium gum, hydroxyethyl cellulose, and guar gum, but not limited thereto. In addition, the specific gravity of the functional suspension modeling crystal ball (4) can be 0.95 to 1, but not limited thereto.

[0049] Please refer to Figure 5 Another embodiment of the present invention provides a method for manufacturing a functional suspended crystal ball. Unless otherwise specified, the meanings of the terms in this embodiment are in principle the same as those in the previous embodiment. The detailed steps of the manufacturing method of this embodiment are as follows.

[0050] First, a mold (1) is provided, wherein the mold has a molding cavity (11) and an opening communicating with the molding cavity (11) (step S21).

[0051] Next, the crystal ball composition liquid (2) is added into the molding cavity (11) of the mold (1) through the opening (step S22). In addition, as mentioned above, in order to facilitate the subsequent demolding of the functional suspended crystal ball from the molding cavity (11) without leaving any residue in the mold (1), the molding cavity (11) of the mold (1) can be wetted with the molding composition liquid before the crystal ball composition liquid (2) is added.

[0052] Finally, the molding composition liquid (3) is added to the crystal ball composition liquid (2) in the molding cavity (11) so as to form a functional suspended molding crystal ball (4) through the molding composition liquid (3) in the molding cavity (11) (steps S23, S24).

[0053] More preferably, the plant extract is a soursop leaf extract. In a preferred embodiment, the preparation method of the soursop leaf extract comprises: first, washing the soursop leaves, drying them in the shade for about 3 to 5 days, and then cutting the soursop leaves into small pieces of about 0.5 cm 2size for extraction. Among them, the extraction rate formula of the present invention is: (extract weight / original total weight of sample)×100%. Next, the chopped mountain soursop leaves are soaked in a soaking step, and this step includes two implementation modes, wherein the first implementation mode is "ultrasonic low-temperature water extraction method", specifically, the implementation mode is: weigh about 100g of mountain soursop leaves and soak them in 900ml of deionized water at 4°C, and use ultrasonic oscillation extraction equipment to continuously extract at low temperature with a total energy of 300-600w. In a preferred embodiment, the continuous extraction is vibrated for a total of 60 minutes in a manner of shaking for 3 minutes and resting for 2 minutes. In another preferred embodiment, after the continuous extraction is completed, high-speed centrifugation is performed at 15,000rpm for 30 minutes at 4°C, the supernatant is collected, and then filtered through a vacuum filter to obtain a clarified filtrate, and the semi-finished extract is concentrated using a reduced pressure concentrator and a freeze dryer to prepare a mountain soursop leaf water extract. In another preferred embodiment, the lyophilized product of the water extract of mountain soursop leaves is stored at -80°C; the second embodiment is "ultrasonic low-temperature ethanol extraction method", specifically, the implementation method is: weigh about 100g of mountain soursop leaves and soak them in five volumes of 95% ethanol, keep them away from light in an environment of 4°C for three days, take them out and continuously extract them at low temperature with a total energy of 300-600w. In a preferred embodiment, the continuous extraction is shaken for a total of 60 minutes in a manner of shaking for 3 minutes and resting for 2 minutes. In another preferred embodiment, after the continuous extraction is completed, centrifuge at 15,000rpm for 30 minutes at 4°C, collect the supernatant, and then filter it through a vacuum filter to obtain a clarified filtrate, and use a vacuum concentrator and a freeze dryer to remove the solvent from the semi-finished extract to prepare a mountain soursop leaf ethanol extract. In another preferred embodiment, the lyophilized product of the mountain soursop leaf ethanol extract is stored at -80°C. Hereinafter, the soursop leaf water extract is referred to as AMLW, and the soursop leaf ethanol extract is referred to as AMLE.

[0054] like Figure 6 As shown, the survival rate of B16F10 cells when the ethanol extract of soursop leaves acts on melanoma cells (B16F10 cells) at 50-1000 μg / ml. Among them, AMLE at 50 μg / ml has significant toxicity to mouse melanoma cells (B16F10), and the cell survival rate is only 30%, indicating that the ethanol extract of soursop leaves has the effect of killing cancer cells and can be used as a raw material for preparing medicines, cosmetics, skin care products, human cleaning products, or fragrance products for treating skin cancer.

[0055] like Figures 7A to 7BThe figure shows the DNA pUC119 experiment after UVB irradiation. When DNA is damaged, it will break from the supercoiled-form (S-form) to the linear-form (L-form). By taking advantage of the different migration speeds of nucleic acids of different molecular weights in the pore size of the colloid, pUC19 DNA was induced by H2O2 (1mM) and UVB (20mJ / cm 2 ) irradiation, if the DNA is protected, it will be in the S-form position, and if it is damaged, the DNA will be in the L-form position. Figures 7A to 7B As shown, it can be found that the control group can maintain a complete DNA morphology and present a coiled structure without damage. In the UVB damage group without drug addition, the DNA structure is damaged and presents a linear structure. When AMLS or AMLW is added first and then irradiated by UVB, a relatively complete DNA coiled structure can be maintained. Therefore, it can be found that the soursop leaf extract has a DNA protection effect, and the water extract of the soursop leaf has a better DNA protection effect than the ethanol extract.

[0056] More preferably, the plant extract is an extract of Allium spicata stem. In a preferred embodiment, the preparation method of the Allium spicata stem extract is the same as the preparation method of the mountain soursop leaf extract, which will not be described in detail here, wherein the “ultrasonic low-temperature water extraction method” is used to prepare an Allium spicata stem water extract; and the “ultrasonic low-temperature ethanol extraction method” is used to prepare an Allium spicata stem ethanol extract. In another preferred embodiment, the plant extract is an Allium spicata leaf extract. In another preferred embodiment, the preparation method of the Allium spicata leaf extract is the same as the preparation method of the mountain soursop leaf extract, which will not be described in detail here, wherein the “ultrasonic low-temperature water extraction method” is used to prepare an Allium spicata leaf water extract; and the “ultrasonic low-temperature ethanol extraction method” is used to prepare an Allium spicata leaf ethanol extract. Hereinafter, the Allium spicata leaf water extract is referred to as ZLLW: the Allium spicata leaf ethanol extract is referred to as ZLLE; the Allium spicata stem water extract is referred to as ZLSW: and the Allium spicata stem ethanol extract is referred to as ZLSE.

[0057] like Figure 8 The figure shows the effect of Allium sibiricum extract on DNA protection. In the group that was only damaged by H2O2, FeSO4 and UVB, the DNA structure was damaged, showing a large number of linear structures. However, the group treated with the Allium sibiricum stem water extract was able to maintain the integrity of the DNA and present a circular winding structure, which has an excellent DNA protection effect.

[0058] like Fig. 9The figure shows the survival rate of B16F10 cells when the extract of Allium argenteae acts on melanoma cells (B16F10 cells) at a concentration of 20-1000 μg / ml, wherein: a control group is provided, the B16F10 cell activity of which is 100%, when ZLLE is added at a concentration of 100 μg / ml or above, the survival rate of B16F10 cells is less than 80%; when ZLSE is added at a concentration of 250 μg / ml or above, the survival rate of B16F10 cells is less than 80%, indicating that it is toxic to B16F10 cells. It indicates that the ethanol extract of Allium argenteae leaves or the ethanol extract of Allium argenteae stems has the effect of killing cancer cells, and can be used to prepare raw materials for the preparation of medicines, cosmetics, skin care products, human cleaning products, or fragrance products for treating skin cancer.

[0059] like Fig.10 As shown in the figures, ZLSE, ZLLE and ZLLW significantly promote the activity of tyrosinase, resulting in an increase in the amount of melanin produced, and the increase rate tends to increase with increasing concentration. Therefore, it is speculated that ZLSE, ZLLE and ZLLW have the ability to promote melanin production and can be used to prepare a composition for promoting melanin production or strengthening hair roots, and the prepared composition can be used as a raw material for self-tanning preparations, scalp care products, shampoos, oral preparations, foods or hair growth water and other products.

[0060] More preferably, the plant extract is a peony stamen growth point tissue extract. In a preferred embodiment, the peony stamen growth point tissue extract comprises a peony stamen growth point tissue water extract or a peony stamen growth point tissue ethanol extract. In another preferred embodiment, the preparation method of the peony stamen growth point tissue water extract comprises: taking fresh peony stamens, rapidly freezing and solidifying the peony stamen tissue with liquid nitrogen at -195.79°C (77K), grinding the peony stamen tissue with a freezing grinder, and grinding the peony stamen tissue into powder; extracting pure water and peony stamen tissue powder in a weight ratio of 9:1, and soaking the peony stamen tissue powder in pure water at a temperature of 4°C to 50°C for 72 to 120 hours; and immersing the mixture of the soaked peony stamen tissue powder and pure water at a temperature of -4°C to 25°C. The extract was continuously and rapidly shaken with a total ultrasonic energy of 500-800w, wherein the shaking was continued in a manner of 5 minutes of shaking and 2 minutes of rest until the total shaking time reached 60-120 minutes; the extract was centrifuged at a high speed of 15,000g for 10 minutes at a temperature condition of -20°C to remove the bottom sediment and collect the supernatant; the supernatant was filtered through a microporous structure filter membrane with a pore size of 0.22-0.45μM, and then filtered using a vacuum screener to obtain a clarified filtrate; the clarified filtrate was freeze-dried using a reduced pressure concentrator and a vacuum freeze dryer to obtain a peony stamen growth point tissue extract (PSB). In another preferred embodiment, the preparation method of the peony stamen growth point tissue ethanol extract comprises: taking fresh peony stamens, freezing and solidifying the peony stamen tissue with liquid nitrogen at -195.79°C (77K), grinding the peony stamen tissue with a freezing grinder, and grinding the peony stamen tissue into powder; extracting an alcohol solution (containing 50% to 75% by weight ethanol) and peony stamen tissue powder in a weight ratio of 9:1, and soaking the peony stamen tissue powder in the alcohol solution (containing 50% to 75% by weight ethanol) at a temperature of 4°C to 50°C for 72 to 120 hours; soaking the soaked peony stamen tissue powder and the alcohol solution (containing 50% to 75% by weight ethanol) in a vacuum oven for 72 to 120 hours; and mixing the soaked peony stamen tissue powder and the alcohol solution (containing 50% to 75% by weight ethanol) with water. The invention relates to a method for preparing a mixture of 1,2-dimethoate (1,2-dimethoate) and 1,2-dimethoate (1,2-dimethoate) of 1,2-dimethoate (1,2-dimethoate) of 1,2-dimethoate (2,2-dimethoate) of 1,2-dimethoate (3,2-dimethoate) of 1,2-dimethoate (4,2-dimethoate) of 1,2-dimethoate (5,2-dimethoate) of 1,2-dimethoate (75 wt. % ethanol), and extracting the mixture by continuous rapid oscillation with a total energy of 500 to 800 W at a temperature of -4°C to 25°C, wherein the oscillation is continuously performed in a manner of 5 minutes of vibration followed by 2 minutes of rest, until the total oscillation time reaches 60 to 120 minutes; centrifuging at a high speed of 15,000 g for 10 minutes at a temperature of -20°C to remove the bottom sediment and collect the supernatant; filtering the supernatant with a microporous structure filter membrane with a pore size of 0.22-0.45 μm and using a vacuum sieve to obtain a clear filtrate; and freeze-drying the clear filtrate using a vacuum concentrator and a vacuum freeze dryer to obtain a peony stamen growth point tissue extract (PSB). The peony stamen growth point tissue extract is hereinafter referred to as PSB.

[0061] PCNA (proliferating cell nuclear antigen) is a proliferating cell nuclear antigen that acts as a DNA clamp, helping DNA polymerase activity (DNA pol) and then recruiting FEN1 (Flap endonuclease 1) to remove the overhanging 5' end. It is the last step of DNA repair involving DNA ligase, which joins the final DNA strands together with phosphodiester bonds. There are many mechanisms to repair damaged double strands. Such as Fig.11 As shown, the control group without UVB damage had a quantitative PCNA expression (1.0), but after UVB irradiation, its expression was significantly reduced (0.2). However, cells treated with peony stamen growth point tissue extract (PSB) significantly increased PCNA expression (0.8). This shows that peony stamen growth point tissue extract can effectively regulate and repair skin cells from UV damage.

[0062] More preferably, the active ingredient is a peony stamen composite microcrystalline capsule composition. In a preferred embodiment, the preparation method of the peony stamen composite microcrystalline capsule composition comprises: mixing a peony stamen growth point tissue extract (PSB) with a carrier raw material, and dispersing the carrier raw material to form a colloidal complex. Specifically, the carrier raw material is dissolved in a solvent, and a rotary vacuum concentrator is used to remove the solvent to form a lipid film, and then the peony stamen growth point tissue extract (PSB) is added to the lipid film to disperse the lipids into a colloidal complex. The obtained colloidal complex is the peony stamen composite microcrystalline capsule composition. The peony stamen composite microcrystalline capsule composition is referred to as PSBL below.

[0063] Mutations in the lamin A gene can damage DNA repair function, making the genome unstable and leading to premature aging. Anti-aging activation mechanism found that Lamin A protein can stimulate the longevity gene SIRT1, allowing cells to self-protect, self-renew and delay aging. Evaluate the expression of anti-aging protein Lamin A by encapsulated peony stamen microcapsules (PSBLs), thereby delaying cell aging. Human skin keratinocytes (HaCaT cells) (1.5×105cell / mL) were cultured in a 10cm diameter dish in a 37℃ and 5% CO2 incubator for at least 24 hours, and encapsulated peony stamen microcapsules (PSBLs) were added and placed in the incubator for 24 hours. When the reaction time was reached, protein extraction was performed using RIPA buffer, followed by SDS-PAGE electrophoresis until the target protein was separated. After adding 0.5% skim milk for 2 minutes, add the first-level antibody for 10 minutes, rinse with 0.1% phosphate buffered saline (PBST) 3 times, add the second-level antibody for 10 minutes, and rinse with 0.1% PBST 3 times. Finally, add ECL cold light sensing reagent, place it in the cold light camera system to take pictures and use image J to quantitatively analyze protein expression. Fig.12 As shown in the figure, human skin keratinocytes (HaCaT cells) were treated with PSBL to analyze the expression of Lamin A protein in the cells. The cytoskeletal protein β-actin was used as a quantitative control group. The results showed that compared with the control group (Lamin A expression was 1.0), the expression of Lamin A increased to 3.8 after PSBL treatment, indicating that PSBL can activate Lamin A protein, which is speculated to have anti-aging effects.

[0064] However, what is described above is only a preferred embodiment of the present invention, but it cannot be used to limit the patent protection scope of the present invention; therefore, all simple equivalent changes and modifications made according to the patent protection scope of the present invention and the contents of the invention specification still fall within the patent protection scope of the present invention.

Claims

1. A composition, characterized in that include: The first liquid layer contains, based on the total weight, 5 to 20 wt% of glycerol, 0.1 to 20 wt% of active ingredients, 1 to 10 wt% of alcohols, and a solvent that makes up to 100 wt%; a second liquid layer comprising, based on the total weight thereof, 5 to 40 wt% of cyclopentasiloxane, 1 to 20 wt% of polydimethylsiloxane, 1 to 20 wt% of phenylpropyldimethylsiloxysilicate, and 1 to 20 wt% of cetearyl polymethicone; and A functional suspended crystal ball, comprising: The main structure is composed of the following ingredients: sodium hyaluronate, polyacrylic acid, sodium alginate, trisaccharide gum, sclerotium gum, hydroxyethyl cellulose, and guar gum; and The active ingredient is a plant extract and is filled in the main structure, wherein: the plant extract is one or more selected from the group consisting of the following extracts: Cinnamomum camphora bud embryo tissue extract, orchid bud embryo tissue extract, Curculigo extract, Longan flower extract, and Chenopodium album extract; an interface is formed between the first liquid layer and the second liquid layer, and the functional suspended crystal ball is suspended on the interface.

2. The composition according to claim 1, characterized in that: The manufacturing method of the functional suspension modeling crystal ball comprises: Providing a mold, the mold having a forming cavity; Adding a crystal ball composition liquid into the molding cavity of the mold, the crystal ball composition liquid containing: the solvent, the main structure, and the active ingredient; and The crystal ball composition liquid in the molding cavity is formed into the functional suspension-shaped crystal ball through the molding composition liquid, and the molding composition liquid contains solvent, calcium carbonate, calcium lactate, calcium chloride, barium chloride, and potassium chloride.

3. The composition according to claim 2, characterized in that Before the crystal ball composition liquid is added to the molding cavity of the mold, the method further includes: The molding cavity of the mold is wetted with the molding composition liquid.

4. The composition according to claim 2, characterized in that: The molding composition liquid contains 0.5 to 2 wt % of calcium carbonate, 0.5 to 2 wt % of calcium lactate, 0.5 to 2 wt % of calcium chloride, 0.5 to 2 wt % of barium chloride, 0.5 to 2 wt % of potassium chloride, and a solvent supplemented to 100 wt % according to its total weight.

5. The composition according to claim 2, characterized in that: The crystal ball composition liquid contains, based on its total weight, 0.01 to 5 wt% of sodium hyaluronate, 0.5 to 5 wt% of polyacrylic acid, 0.5 to 5 wt% of sodium alginate, 0.5 to 5 wt% of Sanxian gum, 0.5 to 2 wt% of sclerotium gum, 0.04 to 2 wt% of hydroxyethyl cellulose, 0.04 to 2 wt% of guar gum, 0.05 to 5 wt% of active ingredients, and a solvent that makes up to 100 wt%.

6. The composition according to claim 1, characterized in that: The specific gravity of the functional suspension modeling crystal ball is 0.95 to 1, the specific gravity of the first liquid layer is 1 to 1.5, and the specific gravity of the second liquid layer is 0.6 to 0.95.

Images