A cultivation device and its anti-condensation method

Abstract

A cultivation device and anti-condensation method belong to the field of intelligent control technology. The cultivation device and anti-condensation method provided by this invention control all heaters installed on the exterior walls of the cultivation device through a single temperature sensor inside the cultivation chamber. This allows all heaters to operate simultaneously within a single heating control cycle. However, different termination times are set based on the different surface conditions of each element in the cultivation chamber (such as heater resistance values ​​and installation position errors, differences in wind speed on the inner walls of the cultivation chamber, and differences in insulation materials on each wall). This solves the problem of mutual interference caused by changes in ambient temperature when individual heaters are controlled independently, and also prevents condensation.

Description

Technical Field

[0001] This invention provides a cultivation device and a method for preventing condensation, belonging to the field of intelligent control technology. Background Technology

[0002] Carbon dioxide is a colorless and odorless gas, although its aqueous solution has a slightly acidic taste. It is also a common greenhouse gas in the air. Carbon dioxide is mainly used for refrigerating perishable foods (solid), as a refrigerant (liquid), in the manufacture of carbonized soft drinks (gaseous), and as a solvent for homogeneous reactions (supercritical state). Carbon dioxide incubators provide an optimal growth environment for cells by maintaining a humid atmosphere with a certain temperature and carbon dioxide concentration.

[0003] Precise control of carbon dioxide concentration is crucial for maintaining a stable pH environment for cell growth and is a key factor for successful cell culture. Currently used carbon dioxide concentration sensors include thermal conductivity and infrared sensors, but humidity and temperature significantly affect the measurement accuracy of thermal conductivity sensors. Therefore, infrared sensors have become the preferred choice. To maintain a sterile environment within the incubator, the incubator interior needs to be sterilized periodically. High-temperature sterilization is widely used due to its superior sterilization effect. However, high-temperature resistant infrared sensors are expensive, and the high-temperature environment inevitably affects the sensor's lifespan and measurement accuracy. Furthermore, particles can affect the measurement accuracy of infrared sensors, so the cleanliness of the measurement atmosphere must be controlled. Therefore, designing a measurement system that can control the number of particles in the gas while avoiding the effects of high temperatures is particularly important. The carbon dioxide incubator is a high-humidity environment, maintained at approximately 95% RH. The dew point temperature in this high-humidity environment is very high, close to the culture temperature. Therefore, the temperature of the inner walls and inner door glass of the incubator must not be lower than the dew point temperature; otherwise, condensation will occur. Water can breed bacteria and cause cross-contamination, leading to cell culture failure. The incubator temperature is provided by heaters on the outer wall and door, which are controlled by temperature sensors. Heaters are installed on all five sides of the outer wall and the surface of the outer door. Each heater is independently controlled by several temperature sensors, most commonly three-way independent temperature control: the outer door temperature sensor controls the outer door temperature, the inner chamber bottom temperature sensor controls the humidification temperature, and the main heating temperature sensor controls the other heaters. Because the outer door and the incubator are sealed by a perimeter sealing strip, heat radiated from the outer door to the inner glass door is lost through the sealing strip. Therefore, the temperature of the inner glass door is affected by the ambient temperature. Higher ambient temperatures result in less heat loss through the sealing strip, leading to a higher inner glass door temperature. This heat transfer from the glass door to the incubator reduces the heat the incubator receives from the other heaters, causing the controller to reduce the heat output of the other heaters. This results in a lower temperature on the inner wall of the incubator, causing condensation. Conversely, lower ambient temperatures result in more heat loss through the sealing strip, causing the inner glass door to become too cold and also condensation. Summary of the Invention

[0004] The main objective of this invention is to provide a cultivation device and its anti-condensation method. All heaters installed on the exterior walls of the cultivation device are controlled by a single temperature sensor inside the cultivation chamber, allowing all heaters to operate simultaneously within a single heating control cycle. However, different end times are set based on the different operating conditions of each surface in the cultivation chamber (such as heater resistance value and installation position error, differences in internal wall wind speed, differences in insulation materials, etc.). This solves the problem of mutual interference caused by changes in ambient temperature when controlled independently, and also prevents condensation.

[0005] To achieve the aforementioned objective, this invention provides a cultivation device comprising a cultivation chamber, wherein heaters are disposed on the outer sides of at least two walls of the cultivation chamber. The device further comprises a carbon dioxide concentration detection structure for measuring the carbon dioxide concentration within the cultivation chamber; a temperature sensor for measuring the temperature within the cultivation chamber; a storage table storing resistance data and heater placement data for the heaters disposed on the outer walls of the cultivation chamber, material properties, thickness, and area data for the inner walls of the cultivation chamber, and wind speed data for the inner walls of the cultivation chamber; and a processor configured to control the operating time of the heaters disposed on the outer walls based on the temperature data, carbon dioxide concentration, resistance data and placement data of the heaters disposed on the outer walls, material properties, thickness, and area data of the inner walls of the cultivation chamber, and wind speed data of the inner walls of the cultivation chamber provided by the temperature sensor.

[0006] To achieve the aforementioned objective, the present invention also provides a method for preventing condensation in a culture device. The culture device is provided with a culture chamber, and heaters are provided on the outer sides of at least two walls of the culture chamber. The working time of the heaters on the outer walls is controlled based on temperature data provided by temperature sensors, carbon dioxide concentration, resistance value data and location data of the heaters on each outer wall, material properties, thickness and area data of each inner wall of the culture chamber, and wind speed data of each inner wall of the culture chamber.

[0007] Compared with the prior art, the present invention has the following beneficial effects:

[0008] All heaters installed on the exterior walls of the cultivation equipment are controlled by a single temperature sensor inside the cultivation chamber, allowing all heaters to operate simultaneously within a single heating control cycle. However, different end times are set based on the different surface conditions of each element in the cultivation chamber (such as heater resistance values ​​and installation position errors, differences in internal wall wind speed, and differences in insulation materials). This solves the problem of mutual interference caused by changes in ambient temperature when using independent control and also prevents condensation. Attached Figure Description

[0009] To more clearly illustrate the technical solutions of the embodiments of the present invention, the accompanying drawings used in the description of the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0010] Figure 1 This is a schematic diagram of the overall structure of the cultivation device provided by the present invention;

[0011] Figure 2 A top view schematic diagram of the culture equipment provided by Benming;

[0012] Figure 3 A front view structural schematic diagram provided by the present invention

[0013] Figure 4 for Figure 2 Schematic diagram of the cross-sectional structure at point AA;

[0014] Figure 5 for Figure 2 Schematic diagram of the cross-sectional structure at point BB;

[0015] Figure 6 This is a block diagram of the control system of the culture device provided by the present invention.

[0016] The attached diagram lists the components represented by each number as follows:

[0017] 1. Culture chamber; 2. Partition; 3. Exhaust pipe; 4. Exhaust pipe; 5. Gas filter; 6. Exhaust pump; 7. Connecting pipe; 8. Inlet pipe; 9. Infrared carbon dioxide concentration sensor; 10. Circulating fan; 11. Delivery pipe; 12. Carbon dioxide delivery pipe; 13. Concentration measuring cylinder; 14. Inlet valve; 15. Connecting flange; 16. Support plate; 17. Support strip; 18. Casters. Detailed Implementation

[0018] The technical solution of the present invention will now be clearly and completely described with reference to the accompanying drawings. Obviously, the described embodiments are only some, not all, of the embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0019] In the description of this invention, it should be noted that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to limit the invention. Unless the context clearly indicates otherwise, the singular forms “a,” “an,” and “the” as used herein are intended to include the plural forms as well. The use of terms such as “comprising” and / or “including” is intended to indicate the presence of that feature, integer, step, operation, element, and / or component, without excluding the presence or addition of one or more other features, integers, steps, operations, elements, components, and / or other combinations. The term “and / or” includes any and all combinations of one or more of the associated listed items. Those skilled in the art will understand the specific meaning of the above terms in this invention based on the specific circumstances.

[0020] Although the exemplary embodiments are described as using multiple modules to perform the exemplary process, it will be understood that the exemplary process may also be performed by one or more units.

[0021] Furthermore, the technical features involved in the different embodiments of the present invention described below can be combined with each other as long as they do not conflict with each other.

[0022] Figure 1 This is a schematic diagram of the overall structure of the cultivation device provided by the present invention; Figure 2 A top view schematic diagram of the culture equipment provided by Benming; Figure 3 This is a front view structural diagram provided by the present invention; Figure 4 for Figure 2 Schematic diagram of the cross-sectional structure at point AA; Figure 5 for Figure 2 Schematic diagram of the cross-sectional structure at point BB;

[0023] like Figure 1-5 As shown, the culture device provided by the present invention includes a culture chamber 1 and a partition 2 fixedly installed on the inner wall of the culture chamber 1. The partition 2 includes an "L"-shaped side plate portion and a bottom plate portion, and a ventilation opening is provided on the top surface of one side of the bottom plate portion. The partition 2 divides the internal space of the culture chamber 1 into two chambers: a culture chamber and an air duct chamber. The air duct chamber is located outside the culture chamber. A circulation convection mechanism is provided on the culture chamber 1.

[0024] In this invention, the culture equipment includes a carbon dioxide supply mechanism, which comprises a delivery connector 11 and a carbon dioxide delivery pipe 12. The delivery connector 11 is embedded in the top surface of the culture chamber 1 and extends to the air duct chamber. The top end of the delivery connector 11 is fitted with the carbon dioxide delivery pipe 12. The carbon dioxide delivery pipe 12 is sequentially provided with a concentration measurement interface 13 and an electrically controlled air inlet valve 14. The concentration measurement interface 13 mainly facilitates personnel to detect the concentration of carbon dioxide introduced into the carbon dioxide delivery pipe 12 using external detection equipment. The electrically controlled air inlet valve 14 is used to control the amount input into the culture chamber. A connecting flange 15 is provided at the end of the carbon dioxide delivery pipe 12 away from the delivery connector 11 for easy connection to a carbon dioxide supply container.

[0025] In this invention, the culture equipment also includes a carbon dioxide concentration detection structure and a processor. The carbon dioxide concentration detection structure measures the carbon dioxide concentration in the culture chamber. The structure includes a gas filter 5, a vacuum pump 6, and an infrared carbon dioxide concentration sensor 9. The vacuum pump 6 draws gas from the culture chamber, filters it through the filter 5, and then delivers it to the carbon dioxide concentration sensor 9. The carbon dioxide concentration sensor 9 measures the concentration of carbon dioxide in the gas and then discharges the gas back into the culture chamber. Specifically, the carbon dioxide concentration detection structure includes a vacuum pipe 4, a gas filter 5, a vacuum pump 6, an infrared carbon dioxide concentration sensor 9, and an inlet pipe 8. One end of the vacuum pipe 4 connects to the culture chamber, and the other end connects to the inlet of the gas filter 5. The exhaust port of the gas filter 5 is connected to the inlet of the vacuum pump 6 via a pipe. The exhaust port of the vacuum pump is connected to the inlet of the carbon dioxide concentration sensor 9. The exhaust port of the carbon dioxide concentration sensor 9 is connected to the culture chamber via the inlet pipe 8. The carbon dioxide concentration sensor provides the acquired carbon dioxide concentration to the processor of the culture equipment. The gas filter 5 mainly filters and controls particulate matter mixed in the carbon dioxide gas.

[0026] In this invention, the culture equipment further includes a circulation convection mechanism, which includes: an air extraction pipe 3, a circulation fan 10, and a pipe 7. One end of the air extraction pipe 3 is embedded in the top surface of one side of the culture chamber 1 and its bottom end extends into the air duct chamber. The other end is connected to the air inlet of the circulation fan 10. The other end of the circulation fan 10 is connected to one end of the pipe 7, and the other end of the pipe 7 is connected to the bottom of the air duct chamber. The circulation fan 10 is used to circulate the gas in the culture chamber and the air duct chamber.

[0027] In this invention, a tray 16 is fixedly installed on one side surface of the culture chamber 1, and two support strips 17 are fixedly installed on the top surface of the tray 16. Each support strip 17 has a movable through hole on one side of its top surface. The exhaust pipe 4 and the inlet pipe 8 respectively movably pass through one support strip 17, and the two support strips 17 respectively provide auxiliary fixation for the exhaust pipe 4 and the inlet pipe 8.

[0028] In this invention, casters 18 are fixedly installed at the corners of the bottom surface of the culture chamber 1. There are four casters 18 in total, which are distributed in a rectangular array.

[0029] In this invention, heaters are provided on the outer surfaces of at least two walls of the culture chamber. Preferably, heaters are provided on the outer surfaces of the upper and lower walls, front and rear walls, left and right walls, and the outer door of the culture chamber. Specifically, a first heater is provided on the outer surface of the upper wall of the culture chamber, a second heater is provided on the outer surface of the front wall of the culture chamber, a third heater is provided on the outer surface of the rear wall of the culture chamber, a fourth heater is provided on the outer surface of the left wall of the culture chamber, and a fifth heater is provided on the outer surface of the right wall of the culture chamber.

[0030] According to one embodiment, aluminum foil is attached to both the culture chamber wall 17 and the heater surface.

[0031] In this invention, the culture device is also equipped with a temperature sensor whose probe extends into the culture chamber, which is used to measure the temperature information of the culture chamber and provide it to the processor.

[0032] Figure 6 A block diagram of the control system of the culture device provided by the present invention is shown below. Figure 6 As shown, the control system includes: a carbon dioxide concentration sensor for measuring the carbon dioxide concentration in the culture chamber; a temperature sensor for measuring the temperature in the culture chamber; a storage table storing the resistance values ​​and location data of each heater, the material properties, thickness, and area data of each inner wall of the culture chamber, and the wind speed data of each inner wall of the culture chamber; and a processor configured to control the operating time of the heaters based on the temperature data, carbon dioxide concentration, heater resistance values ​​and location data, material properties, thickness, and area data of each inner wall of the culture chamber, and wind speed data of each inner wall of the culture chamber provided by the sensors.

[0033] The present invention also provides a method for preventing condensation in a culture device. The culture device is provided with a culture chamber, and heaters are provided on the outer surface of the upper wall, the outer surface of the front and rear walls, the outer surface of the upper and lower walls, and the surface of the outer door of the culture chamber. The method is characterized in that the working time of each heater is controlled according to the temperature data, carbon dioxide concentration, resistance value data and installation location data of the heaters in the culture chamber, material properties, thickness and area data of each inner wall of the culture chamber, and wind speed data of each inner wall of the culture chamber.

[0034] In this invention, the storage table also stores a correspondence table between the working power of the circulating fan and the wind speed of each inner wall of the culture chamber. The correspondence table is obtained by setting an anemometer on each inner wall of the culture chamber and linearly adjusting the working power of the circulating fan to obtain the wind speed of each inner wall of the culture chamber when the circulating fan has different working power.

[0035] In this invention, the processor includes at least a wind speed pointer and an artificial intelligence module. The wind speed pointer is configured to select and provide the wind speed data of each inner wall of the culture chamber corresponding to the working power of the circulating fan to the artificial intelligence module. The artificial intelligence module includes a data processing module, a feature extraction module, and a deep learning module. The data processing module processes the information provided by the temperature sensor and the carbon dioxide concentration sensor to obtain the temperature time series and the carbon dioxide concentration time series. The feature extraction module extracts features from the temperature time series and the carbon dioxide concentration time series respectively and then provides them to the deep learning module. The deep learning module determines the working time of the heaters installed on each outer wall based on the temperature time series, the carbon dioxide concentration time series, and the wind speed of each inner wall of the culture chamber.

[0036] In this invention, the deep learning module includes a neural network, which comprises an input layer, a hidden layer, an output layer, and a decision layer.

[0037] The input layer inputs a temperature-time data sequence of X1 = [x 11 … x n1 … x N1 ] T ;

[0038] The carbon dioxide concentration data sequence is: X4 = [x 14 … x n4 … x N4 ] T ;

[0039] The wind speed data for the inner wall of the first wall of the culture chamber is X5 = [x 15 … x n5 … x N5 ] T ;

[0040] …

[0041] The sequence of wind speed data inside the Mth wall of the culture chamber is X. M =[x 1M … x nM … x NM ] T ;

[0042] The first matrix is ​​obtained by transforming the temperature-time data sequence:

[0043]

[0044] in, x 13 =x 11 ;x n3 =f1(x n1 (x) represents room temperature. n1The functional relationship of the heat required; T is the time interval between adjacent temperature measurements; N≥3;

[0045] The first matrix, the carbon dioxide concentration data sequence, the wind speed data of the upper inner wall of the culture chamber, the wind speed data sequence of the front inner wall of the culture chamber, the wind speed data sequence of the rear inner wall of the culture chamber, the wind speed data sequence of the left inner wall of the culture chamber, and the wind speed data sequence of the right inner wall of the culture chamber are combined to form the second matrix:

[0046]

[0047] In the formula, M is greater than or equal to 2, preferably M = 6;

[0048] The third matrix is ​​obtained by normalizing the second matrix using the normalized coefficient matrix δ.

[0049] Z=I·δ

[0050] Each row of the normalized matrix is ​​input into the input layer of the neural network, and the neurons in the hidden layer of the neural network are activated using a Gaussian function to obtain the first vector:

[0051] Y = [y n1 … y nk … y nK ]

[0052]

[0053] r nm =f k (x nm )+y n(k-1) α k S nm C is the center point of the Gaussian function. nm w is the center point of the Gaussian function km f is the cross-correlation coefficient between the k-th neuron in the hidden layer and the m-th neuron in the input layer; k (x nm x represents the data of the k-th neuron in the hidden layer and the input data of the m-th neuron in the input layer. nm Functional relationship; α k The learning coefficient;

[0054] The output of the neurons in the output layer is represented by a second vector:

[0055] O nj =[q n1 … q nj … q nJ ]

[0056] In the formula J is greater than or equal to 2, q njThe room temperature of the culture room is determined by x (n-1)1 Change to x n1 The heat required to be supplied by the j-th heater on the outer wall of the incubation chamber; w jk Let J be the cross-correlation coefficient between the j-th neuron in the output layer and the k-th neuron in the hidden layer. Preferably, J = M = 6, meaning that a heater is installed on each outer wall of the culture chamber. Optionally, heaters are installed on the outer surfaces of the upper and lower walls, front and rear walls, left and right walls, and the outer door of the culture chamber, where J = 7 and M = 6.

[0057] The output of the neurons in the decision layer is represented by a third vector: P = [p1 … p j … p J ]

[0058] In the formula,

[0059] Optionally,

[0060] ε j For the heat coefficient of the j-th wall of the culture chamber, I j R is the operating current of the j-th heater. j Let be the resistance value of the j-th heater. The time required for all heaters to heat the incubation chamber above the dew point temperature; p j When the value is 1, the j-th heater in the culture chamber continues to work; when the value is 0, it stops working.

[0061] Then, the next set of data is input into the input layer in N steps.

[0062] The control system provided by the present invention also includes an electric valve control driver, which drives the electric valve to open, close, and open to a certain extent according to the control signal provided by the processor.

[0063] The present invention also provides a storage medium for storing a computer program, wherein the computer program is used to implement the methods described above. The memory includes permanent and non-permanent, removable and non-removable media, and information storage can be implemented by any method or technology. The information can be computer-readable instructions, data structures, program modules, or other data. The memory includes, but is not limited to, phase-change memory (PRAM), static random access memory (SRAM), dynamic random access memory (DRAM), other types of random access memory (RAM), read-only memory (ROM), electrically erasable programmable read-only memory (EEPROM), flash memory, CD-ROM, digital versatile optical disc (DVD) or other optical storage, magnetic tape, magnetic magnetic disk storage or other magnetic storage devices, or any other non-transmission medium.

[0064] To achieve the aforementioned objective, the processor provided by this invention is used to execute a computer program, wherein the computer program compiles the above-described method or the steps in the above-described method into a program module.

[0065] In this invention, the temperature sensor can be an external infrared temperature sensor, which avoids the influence of high temperatures. An air filter is set in the carbon dioxide concentration detection structure to remove particles, ensuring measurement accuracy. Through the circulation of the fan and air duct, the carbon dioxide concentration is evenly dispersed throughout the entire space of the culture chamber. This invention uses an artificial intelligence module to repeatedly learn and determine the working time of all heaters set on the outside of each wall of the culture equipment by using data provided by the temperature sensor in the culture chamber, data provided by the carbon dioxide concentration sensor, and wind speed data of each inner wall of the culture chamber. This solves the problem of mutual interference caused by changes in ambient temperature when controlling independently, and also prevents condensation.

[0066] In this invention, the new technical solutions formed by various combinations of the above embodiments are also within the scope of this invention.

[0067] Obviously, the above embodiments are merely illustrative examples for clear explanation and are not intended to limit the implementation. Those skilled in the art will recognize that other variations or modifications can be made based on the above description. It is neither necessary nor possible to exhaustively list all possible implementations here. However, obvious variations or modifications derived therefrom are still within the scope of protection of this invention.

Claims

1. A method for preventing condensation in a culture device, wherein the culture device is provided with a culture chamber, and heaters are provided on the outside of at least two walls of the culture chamber, characterized in that, The method includes: controlling the operating time of heaters installed on the outer walls based on temperature data, carbon dioxide concentration, heater resistance value and location, material properties, thickness and area of ​​each inner wall of the culture chamber, and wind speed data of each inner wall of the culture chamber. Specifically, the operating time of the heaters is determined by an artificial intelligence module, which includes a deep learning module, a neural network, and an input layer, a hidden layer, and an output layer. The temperature-time data sequence is transformed to obtain the first matrix; the first matrix, the carbon dioxide concentration data sequence, and the wind speed data of each inner wall of the incubation chamber are combined to form the second matrix; the second matrix is ​​normalized to obtain the normalized matrix; and each row of the normalized matrix is ​​input into the input layer. The first vector is obtained by activating the neurons in the hidden layer using a Gaussian function: ， ， , and These represent the bandwidth and center point of the Gaussian function, respectively. is the cross-correlation coefficient between the k-th neuron in the hidden layer and the m-th neuron in the input layer; The data of the k-th neuron in the hidden layer and the data input to the m-th neuron in the input layer. The functional relationship; The learning coefficient; The output of neurons in the output layer is represented by a second vector: ， In the formula, J is greater than or equal to 2. The room temperature of the culture room is determined by Change to The heat required to be provided by the j-th heater on the outer wall of the incubation chamber; This is the cross-correlation number between the j-th neuron in the output layer and the k-th neuron in the hidden layer.

2. The method for preventing condensation in the culture equipment according to claim 1, characterized in that, Neural networks also include a decision layer. The output of the neurons in the decision layer is represented by a third vector: ， In the formula, , The thermal coefficient of the j-th wall of the culture chamber, Let j be the operating current of the j-th heater. Let be the resistance value of the j-th heater. The time required for all heaters to heat the incubation chamber above the dew point temperature; When the value is 1, the j-th heater in the culture chamber continues to work; when the value is 0, it stops working. Then, the next set of data is input into the input layer in N steps.

3. The method for preventing condensation in the culture equipment according to claim 2, characterized in that, Based on the operating power of the circulating fan, the wind speed data of each inner wall of the culture chamber corresponding to that operating power is selected using the wind speed pointer.

4. The method for preventing condensation in the culture equipment according to claim 3, characterized in that, The artificial intelligence module also includes a data processing module and a feature extraction module. The data processing module processes the information provided by the temperature sensor and the carbon dioxide concentration sensor to obtain the temperature time series and the carbon dioxide concentration time series; the feature extraction module extracts features from the temperature time series and the carbon dioxide concentration time series respectively.

5. The method for preventing condensation in the culture equipment according to claim 4, characterized in that, Heaters are installed on the outside of the upper and lower walls, the front and rear walls, and the left and right walls of the culture chamber of the culture equipment, or on the outside of the upper and lower walls, the front and rear walls, the left and right walls, and the surface of the outer door of the culture chamber of the culture equipment.

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