Sodium hyaluronate fermentation system and sodium hyaluronate production method using the same

Abstract

The present application relates to sodium hyaluronate processing technical field, especially sodium hyaluronate fermentation system and the sodium hyaluronate production method realized by using it, including a fermenter group, the precision feeder is connected to the feed end of the fermenter group, the precision feeder is used for quantitative feeding to the fermenter group inside, the discharge end of the fermenter group is connected with the output pipeline of pump, the osmotic membrane unit is installed on the output pipeline, the osmotic membrane unit is used to realize the fine purification to the sodium hyaluronate solution generated after fermentation treatment, the fermenter group inside is used to complete the fermentation production of sodium hyaluronate;The primary filtration unit is also installed at the two discharge ends of the fermenter group.The present application is used when the sodium hyaluronate is prepared by fermentation method, and the two fermenter bodies on the fermenter group are interconnected by interconnection components to achieve mutual balance, promote the purpose of reaction, effectively ensure the balance of the reaction inside the two fermenter bodies.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of sodium hyaluronate treatment, in particular to a new process and system capable of realizing rapid fermentation production of sodium hyaluronate and improving fermentation effect and product quality of sodium hyaluronate, especially a sodium hyaluronate fermentation system and a sodium hyaluronate production method realized by using the same. BACKGROUND

[0002] The production process of sodium hyaluronate mainly includes two categories: extraction method using animal tissues as raw materials and bacterial fermentation method. When the extraction method is used to produce sodium hyaluronate, sodium hyaluronate forms a complex with proteins and other mucopolysaccharides in the raw materials, and the separation and purification is complex, and the extraction amount mainly depends on the animal tissue raw materials, which is basically uncontrollable.

[0003] When the fermentation method is used to produce sodium hyaluronate, the existing state is mainly free in the fermentation broth, which is easy to separate and purify, and can be controlled by the parameter conditions of the fermentation method, and the quality is controllable and the yield is unlimited. Therefore, the commonly used method at present is to use the fermentation method to produce and prepare sodium hyaluronate.

[0004] In addition, there are also various related equipment for realizing the production of sodium hyaluronate by using the fermentation method in the prior art. For example, a sodium hyaluronate fermentation tank is disclosed in the patent application No. CN202022636903.2, which mainly includes a tank body, a support column fixedly arranged at the bottom end of the tank body, a discharge pipe fixedly arranged at the middle of the bottom end of the tank body, a discharge valve fixedly arranged at the middle of the discharge pipe, a feeding cylinder fixedly connected with the tank body through a feeding pipe arranged at one side of the top end of the tank body, a feeding valve fixedly arranged at the middle of the feeding pipe, an L-shaped support plate fixedly arranged at the other side of the top end of the tank body, a forward and reverse motor fixedly arranged at the top end of the L-shaped support plate, a connecting lead screw fixedly connected with the transmission shaft of the forward and reverse motor, a moving plate threadedly connected with the connecting lead screw through a lead screw nut, a stirring motor fixedly connected with one end of the moving plate, a stirring shaft fixedly arranged at the transmission shaft of the stirring motor, a through hole formed at the middle of the top end of the tank body, the stirring shaft penetratingly connected with the through hole, stirring rods fixedly arranged at the bottom ends of both sides of the stirring shaft, a heating assembly fixedly arranged at the bottom end of one side of the tank body, and an explosion-proof pipe fixedly arranged at the top end of the other side of the tank body.

[0005] The fermentation tank in the prior art patent above mainly uses a heating assembly inside the tank body to heat the material inside the tank body, which can realize fermentation efficiency in the production process of sodium hyaluronate to a certain extent and accurately grasp the heating temperature of the electric heating wire through the temperature controller to realize accurate temperature control. However, this way of promoting fermentation production does not play a role in the precision treatment of sodium hyaluronate and cannot effectively improve the fermentation effect and the purity of the product. Simply improving the fermentation efficiency of the product has little effect on the quality improvement of the whole production process.

[0006] Therefore, the present application proposes a new process and system capable of realizing rapid fermentation production of sodium hyaluronate and improving the fermentation effect and product quality to better solve the problems existing in the prior art. SUMMARY

[0007] To solve the above technical problems, the technical solution adopted by the present application is as follows: a sodium hyaluronate fermentation system includes a fermentation tank group, a precision feeder is connected to the feed end of the fermentation tank group, the precision feeder is used for quantitative feeding to the inside of the fermentation tank group, a pump-equipped output pipeline is connected to the discharge end of the fermentation tank group, a permeation membrane unit is installed on the output pipeline, the permeation membrane unit is used for realizing fine purification of the sodium hyaluronate solution generated after fermentation treatment, and the fermentation tank group is used for completing the fermentation production of sodium hyaluronate; a primary filtration unit is also installed at both discharge ends of the fermentation tank group.

[0008] In any of the above schemes, preferably, the primary filtration unit includes a primary filtration cartridge installed at the corresponding discharge end of the fermentation tank group, a primary filtration check valve is installed at the upper inlet end of the primary filtration cartridge, a push piston is installed in the inner cavity of the primary filtration cartridge, the top of the push piston is upwardly movable and seals through a detachable top cover of the primary filtration cartridge and is connected to the piston rod of a fixedly arranged primary filtration push cylinder, a primary filtration screen is detachably and fixedly installed in the middle cavity of the primary filtration cartridge, and a primary filtration liquid discharge pipe with a valve connected to the output pipeline outside is installed on the primary filtration cartridge below the primary filtration screen.

[0009] In any of the above schemes, preferably, the precision feeder includes a screw feeder, a feed main pipe is connected to the bottom outlet of the screw feeder, the bottom of the feed main pipe is connected to two feed branch pipes through a tee joint, the ends of the two feed branch pipes are respectively connected to the two feed ends of the fermentation tank group, and a feed check valve is installed on each feed branch pipe.

[0010] Preferably, in any of the above solutions, the fermenter set comprises two fermenter bodies arranged in parallel, the sodium hyaluronate solution is prepared in each fermenter body by fermentation, and a feed branch is connected to the top of each fermenter body.

[0011] Preferably, in any of the above solutions, a thermometer and a pressure gauge are arranged on each fermenter body.

[0012] Preferably, in any of the above solutions, the two fermenter bodies are connected by an interconnection and intercommunication component, the interconnection and intercommunication component comprises linkage pistons sealingly arranged in the reaction cavities of the two fermenter bodies, the linkage pistons are movably sealingly fitted in the corresponding reaction cavities, the reaction cavities above the linkage pistons are respectively filled with reaction liquid and bacteria, the reaction cavities below the linkage pistons are divided into buffer variable cavities, the buffer variable cavities are connected by an intercommunication pipeline, a vertical light shaft is fixedly connected to the bottom of each linkage piston, the lower end of the vertical light shaft movably and sealingly penetrates through the bottom of the fermenter body, a supporting spring is sleeved on the outer wall of the vertical light shaft in each buffer variable cavity, the top of the supporting spring supports the bottom of the linkage piston at the corresponding position, and the bottom of the supporting spring supports the bottom of the fermenter body.

[0013] Preferably, in any of the above solutions, a normally open heating control switch is arranged below each vertical light shaft, and the two heating control switches are connected to the heaters in the interiors of the fermenter bodies on the adjacent sides of the heating control switches in a cross control manner, that is, when the heating control switch on the left side is turned on, the heater in the interior of the fermenter body on the right side is controlled to heat the reaction liquid, and when the heating control switch on the right side is turned on, the heater in the interior of the fermenter body on the left side is controlled to heat the reaction liquid.

[0014] Each heating control switch is double-controlled connected to the heating switch on the fermenter body corresponding to the heating control switch.

[0015] Preferably, in any of the above solutions, the permeation membrane unit set comprises a plurality of permeation filtration units arranged in parallel, the two ends of each permeation filtration unit are connected to the output pipeline through permeation branch pipelines, and a branch valve is arranged on each permeation branch pipeline, each branch valve is used to control the opening and cutting off of the current permeation membrane unit.

[0016] The application further provides a sodium hyaluronate production method using the sodium hyaluronate fermentation system, which comprises the following steps.

[0017] Put the appropriate amount of bacteria and reaction material and reaction liquid into the precision feeder;

[0018] Control the operation of the precision feeder and sequentially put the appropriate amount of reaction liquid, material and bacteria into the two fermentation tank bodies;

[0019] Control the heating switch on the two fermentation tank bodies to be turned on and heat the reaction cavity inside the fermentation tank body, and observe the changes of the thermometer and pressure gauge on the fermentation tank body;

[0020] When the reaction in the reaction cavity is carried out by fermentation method, the internal pressure changes, and when the reaction speeds in the two fermentation tank bodies are inconsistent, the interconnection component will regulate to reduce the reaction gap between the two, so that the reactions in the two fermentation tank bodies are in a balanced state.

[0021] After the reaction is completed, the corresponding pipeline valve is opened, and the initial filtration unit and the permeation membrane unit are used to complete the filtration and purification of the sodium hyaluronate solution after the reaction. After filtration and purification, the sodium hyaluronate solution is collected by the external collection device.

[0022] Compared with the prior art, the beneficial effects of the present application are as follows:

[0023] 1. When the fermentation system is used for fermentation of sodium hyaluronate, the two fermentation tank bodies on the fermentation tank group are interconnected by the interconnection component to achieve mutual balance and promote reaction, effectively ensuring the balance of the reactions in the two fermentation tank bodies.

[0024] 2. After the reaction liquid after the reaction is purified and filtered, the initial filtration unit and the permeation membrane unit can effectively improve the precision of the sodium hyaluronate reaction liquid and reduce the content of impurities in it.

[0025] 3. The parallel structure design of the multiple groups of the permeation membrane unit can effectively ensure that the operation of the entire permeation membrane unit is not affected when one or part of the permeation filtration units in the permeation membrane unit fails, and ensure the continuous operation of the permeation membrane unit. BRIEF DESCRIPTION OF DRAWINGS

[0026] In order to more clearly illustrate the specific embodiments of the present application or the technical solutions in the prior art, the following will briefly introduce the drawings needed to be used in the specific embodiments or the prior art description. In all the drawings, similar elements or components are generally identified by similar reference numerals. In the drawings, the elements or components are not necessarily drawn according to the actual proportions.

[0027] Figure 1 It is an internal structure diagram of the present application.

[0028] Figure 2It is the internal section structure schematic view of the fermenting tank group of the present application.

[0029] Figure 3 It is the internal structure schematic view of the fermenting tank body of the present application.

[0030] Figure 4 It is the structure schematic view of the permeation membrane unit + primary filtration unit of the present application.

[0031] In the figure, 1, fermenting tank group; 2, output pipeline; 3, permeation membrane unit; 4, primary filtration unit; 401, primary filtration cylinder; 402, primary filtration one-way valve; 403, push piston; 404, primary filtration push cylinder; 405, primary filtration liquid discharge pipe; 5, screw feeder; 6, feed main pipe; 7, feed branch pipe; 8, feed one-way valve; 9, fermenting tank body; 10, thermometer; 11, pressure gauge; 12, reaction cavity; 13, linkage piston; 14, buffer variable cavity; 15, intercommunication pipeline; 16, vertical optical shaft; 17, support spring; 18, heating control switch; 19, heater; 20, permeation filtration unit; 21, permeation branch pipeline; 22, branch valve; 23, agitator; 24, agitator motor. DETAILED DESCRIPTION

[0032] The embodiments of the technical scheme of the present application will be described in detail below with reference to the accompanying drawings. The following embodiments are only used to more clearly illustrate the technical scheme of the present application, thus only serve as examples, and cannot limit the protection scope of the present application. The specific structure of the present application is shown in Figures 1-4 .

[0033] Embodiment 1:

[0034] The present application is used to solve one of the above technical problems, and the technical scheme adopted is: a sodium hyaluronate fermentation system, comprising a fermenting tank group 1, a precision feeder connected to the feed end of the fermenting tank group 1, the precision feeder being used for quantitative feeding into the fermenting tank group 1, a pump-equipped output pipeline 2 connected to the discharge end of the fermenting tank group 1, a permeation membrane unit 3 installed on the output pipeline 2, the permeation membrane unit 3 being used for realizing fine purification of the sodium hyaluronate solution generated after fermentation treatment, the fermenting tank group 1 being used for completing the fermentation production of sodium hyaluronate; a primary filtration unit 4 is also installed at both discharge ends of the fermenting tank group 1. The sodium hyaluronate fermentation system uses the precision feeder to perform accurate feeding, and the feeding amount can be controlled as needed when feeding; after the material enters the fermenting tank group 1, it performs fermentation reaction inside and generates a sodium hyaluronate solution, and the primary filtration unit 4 and the permeation membrane unit 3 can filter and purify the impurities in the output sodium hyaluronate solution, effectively ensuring the purity of the sodium hyaluronate solution.

[0035] In any of the above solutions, preferably, the primary filter unit 4 comprises a primary filter cylinder 401 installed at the corresponding discharge end of the fermenter group 1, a primary filter one-way valve 402 installed at the upper inlet end of the primary filter cylinder 401, a push piston 403 installed in the inner cavity of the primary filter cylinder 401, the top of the push piston 403 upwardly movable and sealingly passing through a detachable top cover of the primary filter cylinder 401 and connected to the piston rod of a fixedly installed primary filter push cylinder 404, a primary filter screen 404 detachably and fixedly installed in the middle cavity of the primary filter cylinder 401, and a primary filter liquid discharge pipe 405 with a valve installed at the primary filter cylinder 401 below the primary filter screen 404 and connected to the external output pipeline 2. The primary filter unit 4 performs primary filtration on the sodium hyaluronate solution entering the inner part of the primary filter cylinder 401. During filtration, the primary filter push cylinder is directly started to drive the push piston 403 to reciprocally and downwardly press in the primary filter cylinder 401. In the process of downward pressing, the solution passes through the primary filter screen 404 to enter the lower part and is discharged by the primary filter liquid discharge pipe 405, while the particulate impurities are blocked above the primary filter screen 404. Regular cleaning of the primary filter screen 404 can clean the accumulated impurities.

[0036] In any of the above solutions, preferably, the precision feeder comprises a screw feeder 5, the bottom outlet of which is connected to a feed main pipe 6, the bottom of the feed main pipe 6 is connected to two feed branch pipes 7 through a three-way pipe, the distal ends of the two feed branch pipes 7 are respectively connected to the two feed ends of the fermenter group 1, and a feed one-way valve 8 is installed on each of the feed branch pipes 7. The screw feeder 5 can effectively deliver the material directly to the corresponding feed branch pipe 7, and then to the inner part of the fermenter group 1. The feed one-way valve 8 can effectively control the reaction cutoff to ensure the sealing during fermentation in the fermenter group 1.

[0037] In any of the above solutions, preferably, the fermenter group 1 comprises two fermenter bodies 9 arranged at intervals, sodium hyaluronate solution is prepared in the inner part of each of the fermenter bodies 9, the top of each of the fermenter bodies 9 is respectively connected to the corresponding position of the feed branch pipe 7, and each of the feed branch pipes 7 delivers the bacterial strain and the reaction liquid to the inner part of the corresponding fermenter body 9. The reactions in the two fermenter bodies 9 are independent of each other, but the reaction effect and efficiency in the two fermenter bodies 9 can be kept relatively balanced by cooperating with the interconnection and intercommunication components, so as to ensure the balanced efficiency of preparing sodium hyaluronate solution in the two reaction cavities 12.

[0038] In any of the above solutions, preferably, a thermometer 10 and a pressure gauge 11 are respectively installed on the fermenter body 9. The thermometer 10 and the pressure gauge 11 can facilitate the observation of the temperature and pressure conditions in the inner part of the reaction cavity 12.

[0039] In any of the above schemes, preferably, the two fermentation tanks 9 are interconnected by an interconnection communication component, which includes linkage pistons 13 respectively sealingly installed in the reaction cavities 12 of the two fermentation tanks 9, the linkage pistons 13 are in movable sealing cooperation with the corresponding reaction cavities 12, the reaction cavities 12 above the linkage pistons 13 respectively contain reaction liquid and bacteria, the reaction cavities 12 below the linkage pistons 13 are divided into buffer variable cavities 14, the two buffer variable cavities 14 are communicated by a communication pipeline 15, a vertical light shaft 16 is fixedly connected to the center bottom of each linkage piston 13, the lower end of the vertical light shaft 16 movably and sealingly penetrates through the bottom of the fermentation tank 9, a supporting spring 17 is sleeved on the outer side wall of the vertical light shaft 16 in each buffer variable cavity 14, the top of the supporting spring 17 supports the bottom of the linkage piston 13 at the corresponding position, and the bottom of the supporting spring 17 supports the bottom of the fermentation tank 9. In operation, the interconnection communication component mainly utilizes the pressure increase of the reaction cavity 12 caused by the heat release of the reaction in the corresponding reaction cavity 12 to realize the downward movement of the linkage piston 13 in the equilibrium state below the reaction cavity 12. If the reaction efficiencies in the two fermentation tanks 9 are the same and the pressures on both sides are the same, the two linkage pistons 13 are still in a relatively balanced and static state. When the reaction in one of the fermentation tanks 9 is too fast, the pressure in the reaction cavity 12 of the fermentation tank 9 increases more greatly, the pressure in the reaction cavity 12 pushes the linkage piston 13 to overcome the elastic force of the supporting spring 17, and the linkage piston 13 moves downward. Since the two buffer variable cavities 14 formed by the two linkage pistons 13 and the communication pipeline 15 are sealingly communicated, when the linkage piston 13 on one side moves downward, the linkage piston 13 on the other side is forced to move upward, so that the volume of the reaction cavity 12 on the other side is compressed and the air pressure is increased, thereby promoting the reaction on the side and making the reactions in the two reaction cavities 12 tend to be balanced.

[0040] Embodiment 2:

[0041] In order to solve the above technical problems, the technical scheme adopted by the present application is as follows: a sodium hyaluronate fermentation system, which comprises a fermentation tank group 1, a precision feeder connected to the feeding end of the fermentation tank group 1, the precision feeder being used for quantitative feeding into the fermentation tank group 1, a pump-equipped output pipeline 2 connected to the discharging end of the fermentation tank group 1, a permeation membrane unit 3 installed on the output pipeline 2, the permeation membrane unit 3 being used for realizing fine purification of the sodium hyaluronate solution generated after fermentation treatment, and the fermentation tank group 1 being used for completing the fermentation production of sodium hyaluronate; an initial filtration unit 4 is also installed at each discharging end of the fermentation tank group 1, and a power pump is installed on each corresponding pipeline as needed.

[0042] The precision feeder is used for precise feeding in the sodium hyaluronate fermentation system, and the feeding amount can be controlled as required during feeding; after the material enters the fermentation tank group 1, the fermentation reaction is carried out in the fermentation tank group 1, and the sodium hyaluronate solution is generated; the impurities in the output sodium hyaluronate solution can be filtered and purified by using the primary filtration unit 4 and the osmotic membrane unit 3, so that the purity of the sodium hyaluronate solution is effectively ensured.

[0043] In any of the above schemes, preferably, the primary filtration unit 4 comprises a primary filter cartridge 401 installed at the corresponding discharge end of the fermentation tank group 1, a primary filter one-way valve 402 is installed at the upper inlet end of the primary filter cartridge 401, a push piston 403 is installed in the inner cavity of the primary filter cartridge 401, the top of the push piston 403 is movable upward and seals through a detachable top cover of the primary filter cartridge 401 and is connected with a piston rod of a primary filter push cylinder 404 fixedly arranged, a primary filter screen 404 is detachably and fixedly installed in the middle cavity of the primary filter cartridge 401, and a primary filter liquid discharge pipe 405 with a valve connected with the external output pipeline 2 is installed below the primary filter screen 404 of the primary filter cartridge 401.

[0044] The primary filtration unit 4 performs primary filtration on the sodium hyaluronate solution entering the inside of the primary filter cartridge 401, and directly starts the filter push cylinder to drive the push piston 403 to reciprocatingly press downward in the primary filter cartridge 401 during filtration. In the process of pressing downward, the solution passes through the primary filter screen 404 to enter below and is discharged by the primary filter liquid discharge pipe 405, and the particulate impurities are blocked above the primary filter screen 404, so that the accumulated impurities can be cleaned by regularly cleaning the primary filter screen 404.

[0045] In any of the above schemes, preferably, the precision feeder comprises a screw feeder 5, a feed main pipe 6 is connected to the bottom outlet of the screw feeder 5, the bottom of the feed main pipe 6 is connected with two feed branch pipes 7 through a three-way pipe, and the ends of the two feed branch pipes 7 are respectively connected to the two feed ends of the fermentation tank group 1. The feed one-way valve 8 is installed on each of the feed branch pipes 7.

[0046] The screw feeder 5 can effectively deliver the material directly to the corresponding feed branch pipe 7, and then to the inside of the fermentation tank group 1, and the feed one-way valve 8 can effectively control the reaction cutoff to ensure the sealing property during the fermentation in the fermentation tank group 1.

[0047] In any of the above schemes, preferably, the fermentation tank group 1 comprises two fermentation tank bodies 9 arranged at intervals, sodium hyaluronate solution is prepared in the inside of each of the fermentation tank bodies 9 by fermentation, the top of each of the fermentation tank bodies 9 is respectively connected with the corresponding position of the feed branch pipe 7, and each of the feed branch pipes 7 delivers the bacteria and the reaction liquid to the inside of the corresponding position of the fermentation tank body 9.

[0048] The reactions in the two fermentation tanks 9 are independent of each other, but the interconnection and intercommunication component can keep the reaction effects and efficiency of the two fermentation tanks 9 relatively balanced, thereby ensuring the balance of the efficiency of the preparation of the sodium hyaluronate solution in the two reaction cavities 12.

[0049] In any of the above solutions, preferably, a thermometer 10 and a pressure gauge 11 are respectively installed on the fermentation tanks 9.

[0050] The thermometer 10 and the pressure gauge 11 can facilitate the observation of the temperature and pressure in the reaction cavity 12.

[0051] In any of the above solutions, preferably, the two fermentation tanks 9 are interconnected by an interconnection and intercommunication component, which comprises linkage pistons 13 respectively sealingly installed in the reaction cavities 12 of the two fermentation tanks 9. The linkage pistons 13 are movably sealingly fitted in the corresponding reaction cavities 12. The reaction cavities 12 above the linkage pistons 13 respectively contain reaction liquid and bacteria. The reaction cavities 12 below the linkage pistons 13 are divided into buffer variable cavities 14. The two buffer variable cavities 14 are connected by an intercommunication pipeline 15. A vertical light shaft 16 is fixedly connected to the center bottom of each linkage piston 13. The lower end of the vertical light shaft 16 movably and sealingly penetrates through the bottom of the fermentation tank 9. A supporting spring 17 is sleeved to the outer sidewall of the vertical light shaft 16 in each buffer variable cavity 14. The top of the supporting spring 17 supports the bottom of the linkage piston 13 at the corresponding position, and the bottom of the supporting spring 17 supports the bottom of the fermentation tank 9.

[0052] In operation, the interconnection and intercommunication component mainly utilizes the pressure increase of the reaction cavity 12 caused by the heat generated by the reaction in the corresponding reaction cavity 12 to push the linkage piston 13 below to move downward. If the reaction efficiency in the two fermentation tanks 9 is the same, the pressure on both sides is the same, and at this time, the two linkage pistons 13 are still in a relatively balanced and stationary state. When the reaction in one of the fermentation tanks 9 is too fast, the pressure in the reaction cavity 12 of this side will increase more greatly. At this time, the pressure in the reaction cavity 12 will push the linkage piston 13 to overcome the elastic force of the supporting spring 17 at this position, and make the linkage piston 13 move downward. At this time, since the two buffer variable cavities 14 formed by the two linkage pistons 13 and the intercommunication pipeline 15 are sealingly communicated, when the linkage piston 13 on one side moves downward, it will force the linkage piston 13 on the other side to move upward, thereby compressing the volume of the reaction cavity 12 on the other side and increasing the air pressure, so as to accelerate the reaction on this side and make the reactions in the two reaction cavities 12 tend to be balanced.

[0053] In any of the above schemes, preferably, a normally open heating control switch 18 is arranged below each vertical light axis 16, and the two heating control switches 18 are connected to the heaters 19 in the interiors of the adjacent sides of the fermentation tank bodies 9 in a cross control manner, that is, when the left heating control switch 18 is turned on, the heater 19 in the interior of the right fermentation tank body 9 is controlled to heat the reaction liquid, and when the right heating control switch 18 is turned on, the heater 19 in the interior of the left fermentation tank body 9 is controlled to heat the reaction liquid.

[0054] Each heating control switch 18 is double-controlled connected to the heating switch on the corresponding fermentation tank body 9.

[0055] When the linkage piston 13 moves downward, it drives the vertical light axis 16 on the same side to move downward, thereby pressing the heating control switch 18 of the fermentation tank body 9 on the other side to turn on, so that the heater 19 in the interior of the fermentation tank body 9 on the side with slower reaction heats up, the temperature in the interior of the reaction chamber 12 on the side is increased, thereby achieving the purpose of secondary promoting the reaction rate. After heating, the reaction efficiency in the interior of the reaction chamber 12 on the side is increased, and the reaction in the interiors of the two reaction chambers 12 is balanced again through such reciprocating movement.

[0056] In any of the above schemes, preferably, the permeation membrane unit 3 includes a plurality of parallelly arranged permeation filtration units 20, both ends of each permeation filtration unit 20 are connected to the output pipeline 2 through permeation branch pipelines 21, and a branch valve 22 is installed on each permeation branch pipeline 21, each branch valve 22 is used to control the opening and cutting of the current permeation membrane unit 3.

[0057] The parallel structure design of the plurality of groups of the permeation membrane unit 3 can effectively ensure that the operation of the entire permeation membrane unit 3 is not affected when one or part of the permeation filtration units 20 in the permeation membrane unit 3 fails, and ensure the continuous operation of the permeation membrane unit 3.

[0058] In any of the above schemes, preferably, a stirrer 23 is installed in the reaction chamber 12 of each fermentation tank body 9, the top of the stirrer 23 is upwardly movable out of the top of the fermentation tank body 9 and is controlled and driven by a fixedly arranged stirring motor 24.

[0059] When the stirring motor 24 is driven, it can drive the stirrer 23 to stir and drive the reaction liquid in the interior of the reaction chamber 12, thereby effectively ensuring the uniformity of the reaction liquid.

[0060] The sodium hyaluronate production method realized by the sodium hyaluronate fermentation system includes the following steps:

[0061] The appropriate amount of bacteria and reaction materials and reaction liquid are put into the precision feeder;

[0062] The precision feeder is controlled to operate and sequentially put the appropriate amount of reaction liquid, materials and bacteria into the two fermentation tanks 9;

[0063] The heating switch on the two fermentation tanks 9 is controlled to be turned on and the reaction cavity 12 of the fermentation tank 9 is heated, and the changes of the thermometer 10 and the pressure gauge 11 on the fermentation tank 9 are observed;

[0064] When the fermentation method is used to react in the reaction cavity 12, the internal pressure changes, and when the reaction speeds in the two fermentation tanks 9 are inconsistent, the interconnection and intercommunication component can be controlled to reduce the reaction difference between the two fermentation tanks 9, so that the reactions in the two fermentation tanks 9 are in a balanced state.

[0065] After the reaction is completed, the corresponding pipeline valve is opened, and the initial filter unit 4 and the permeation membrane unit 3 are used to filter and purify the sodium hyaluronate solution after the reaction, and the sodium hyaluronate solution is collected by the external collection device after the filtration and purification.

[0066] The above embodiments are only used to illustrate the technical solutions of the present application, but not to limit it; although the present application has been described in detail with reference to the above embodiments, those skilled in the art should understand that the technical solutions recorded in the above embodiments can be modified, or some or all of the technical features can be replaced equivalently; and these modifications or replacements do not make the essence of the corresponding technical solutions deviate from the scope of the technical solutions of the embodiments of the present application, and they should be covered in the scope of the claims and the specification of the present application; any alternative improvement or change made by those skilled in the art to the embodiments of the present application falls within the protection scope of the present application.

[0067] The details of the present application not described are well known to those skilled in the art.

Claims

1. A sodium hyaluronate fermentation apparatus, characterized in that: The system includes a fermentation tank assembly. A precision feeder is connected to the feed inlet of the fermentation tank assembly for quantitatively feeding materials into the fermentation tank assembly. The discharge end of the fermentation tank assembly is connected to an output pipeline with a pump. A permeation membrane unit is installed on the output pipeline for fine purification of the sodium hyaluronate solution generated after fermentation. The fermentation tank assembly is used to complete the fermentation production of sodium hyaluronate. Primary filtration units are also installed at both discharge ends of the fermentation tank assembly. Two fermentation tanks are interconnected via an interconnecting component. The interconnecting component includes a linkage piston that is sealed and installed in the reaction chambers of the two fermentation tanks respectively. The linkage piston and the corresponding reaction chamber are in a movable sealed fit. The reaction chamber above each linkage piston contains reaction liquid and inoculum. The reaction chamber below each linkage piston is divided into a buffer variable chamber. The two buffer variable chambers are connected by an interconnecting pipe. A vertical optical shaft is fixedly connected to the bottom center of each linkage piston. The lower end of the vertical optical shaft moves and is sealed through the bottom of the fermentation tank. A support spring is sleeved on the outer wall of the vertical optical shaft in each buffer variable chamber. The top of the support spring supports the bottom of the linkage piston at the corresponding position, and the bottom of the support spring supports the bottom of the fermentation tank. When the interconnected components are in operation, they utilize the heat release and pressure increase inside the corresponding reaction chamber to push the linkage piston below it, which is in a state of equilibrium, downward. If the reaction efficiency inside the two fermentation tanks is the same, the pressure on both sides is the same, and the two linkage pistons will still be in a relatively balanced and static state. When the reaction inside one of the fermentation tanks is too fast, the pressure inside its reaction chamber increases more significantly. The pressure inside that reaction chamber will push the linkage piston to overcome the elastic force of the support spring at that point, causing the linkage piston to move downward. Since the two linkage pistons and the interconnecting pipes form two separate buffer variable chambers that are sealed and interconnected, when the linkage piston on one side moves downward, it will force the linkage piston on the other side to move upward, which will compress the volume of the reaction chamber on the other side, increase the gas pressure, promote the acceleration of the reaction on that side, and promote the reaction in the two reaction chambers to tend to be in equilibrium. The permeation membrane unit includes several permeation filtration units arranged in parallel. Both ends of each permeation filtration unit are connected to the output pipeline through permeation branch pipes. Each permeation branch pipe is equipped with a branch valve, which is used to control the opening and closing of the current permeation membrane unit.

2. The sodium hyaluronate fermentation apparatus according to claim 1, characterized in that: The primary filtration unit includes a primary filter cartridge installed at the discharge end of the fermenter group. A primary filter check valve is installed at the upper inlet end of the primary filter cartridge. A push piston is installed in the inner cavity of the primary filter cartridge. The top of the push piston moves upward and seals through the detachable top cover of the primary filter cartridge and is connected to the piston rod of a fixedly installed primary filter push cylinder. A primary filter screen is detachably and fixedly installed in the middle section of the primary filter cartridge. A primary filter drain pipe with a valve is installed on the primary filter cartridge below the primary filter screen and connected to an external output pipeline.

3. The sodium hyaluronate fermentation apparatus according to claim 2, characterized in that: The precision feeder includes a screw conveyor, with a main feed pipe connected to the bottom outlet of the screw conveyor. The bottom of the main feed pipe is connected to two feed branch pipes via a tee. The ends of the two feed branch pipes are respectively connected to the two feed ends of the fermentation tank group. A one-way valve is installed on each feed branch pipe.

4. The sodium hyaluronate fermentation apparatus according to claim 3, characterized in that: The fermentation tank group includes two spaced-apart fermentation tanks. Sodium hyaluronate solution is prepared inside each fermentation tank by fermentation. Each fermentation tank has a feed branch pipe connected to the top of it at a corresponding position. Each feed branch pipe delivers the inoculum and reaction solution into the fermentation tank at the corresponding position.

5. The sodium hyaluronate fermentation apparatus according to claim 4, characterized in that: A thermometer and a pressure gauge are installed on the fermentation tank.

6. The sodium hyaluronate fermentation apparatus according to claim 5, characterized in that: Below each vertical optical axis, there is a normally open heating control switch. The two heating control switches are connected to the heaters inside the fermentation tank on their respective adjacent sides by cross control. That is, when the heating control switch on the left is turned on, it controls the heater inside the fermentation tank on the right to heat the reaction liquid. When the heating control switch on the right is turned on, it controls the heater inside the fermentation tank on the left to heat the reaction liquid. Each of the heating control switches is connected to the corresponding heating switch on the fermentation tank body for dual control.

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