A method for indoor propagation of black hooked butterfly

Abstract

The present invention discloses a method for indoor propagation of black hooked hessians. The method comprises the steps of egg collection, egg hatching / larval rearing, and adult rearing. The method uses Amomum villosum leaves as an oviposition substrate, introduces adult black hooked hessians for mating and egg-laying culture, collects the oviposition substrate carrying black hooked hessians eggs, places it in an insect box containing an antibacterial and moisturizing substrate for hatching and larval culture, and collects the pupae when they emerge, and rears the adults. The method adds a traditional Chinese medicine solution made from Dai Baijie, Gynostemma pentaphyllum, and Kidney Tea, which can reduce the fungal infection rate by 29%, increase the survival rate of black hooked hessians, streamline the rearing steps, and facilitate standardized rearing of black hooked hessians. The propagation method is simple and practical, and a large number of black hooked hessians of uniform age can be obtained in a short period of time, laying a foundation for theoretical research on the biology, ecology, and toxicology of black hooked hessians.

Description

Technical Field

[0001] The invention belongs to the technical field of insect breeding, and particularly relates to an indoor propagation method for black hooked hesperi. Background Art

[0002] Amomum villosum Amomum villosum Lour Widely cultivated in Yunnan, Guangdong, Hainan, and Guangxi, Amomum villosum is one of my country's four renowned southern medicinal herbs. Yunnan accounts for over 60% of the country's total production and is one of the ten renowned Yunnan medicinal herbs. It boasts benefits such as strengthening the stomach and spleen, regulating qi, and stabilizing pregnancy. It is also a key Dai medicinal herb widely cultivated by farmers in regions such as Xishuangbanna Prefecture in Yunnan. However, with the continuous expansion of cultivated areas, the infestation of Amomum villosum insects has become increasingly rampant, severely impacting its yield and quality, hindering its standardized cultivation and the sustainable development of the related industrial chain.

[0003] Black hook butterfly ( Ancistroides nigrita Latreille is a genus of the Lepidoptera family Hesperidae ( AncistroidesButler ), a new pest of Amomum vulgare, discovered for the first time by the applicant in Xishuangbanna Dai Autonomous Prefecture, Yunnan Province, is characterized by its wide distribution, strong reproductive capacity, and concealed nature. This insect is widely distributed in Thailand, Cambodia, Myanmar, Malaysia, Indonesia, the Philippines, India, Bangladesh, and Bhutan, and is primarily found in Yunnan and Hainan in China. The black hooked hesperid's host plants include Amomum vulgare, ginger, and other plants in the Zingiberaceae family.

[0004] The larvae of the black hooked butterfly primarily infest the leaves of Amomum villosum. They lay their eggs in the leaves of Amomum villosum. Newly hatched larvae prefer to feed on the young leaves, often creating numerous irregular notches. Older larvae enter a period of gluttony, allowing them to shift their damage. When attacking Amomum villosum, the larvae often spin silk to regularly sew the leaves together at the margins, forming dumpling-shaped capsules within which they feed and develop, making their damage highly concealed. In severe cases, large capsules can form on the leaves of Amomum villosum plants, completely devouring the leaves, causing the entire plant to wither or even die. Therefore, conducting basic theoretical research on the biology and ecology of the black hooked butterfly is crucial and a key component of developing a key technology framework for green pest control of Amomum villosum.

[0005] Currently, only a few descriptions of the morphological characteristics of the black hooked hesperid butterfly exist in China, and no reports exist on its biological characteristics or rearing techniques. Establishing a stable experimental population of this butterfly indoors and obtaining a large, consistent, standardized source for research lays the foundation for systematic studies on its biology, ecology, toxicology, and integrated control techniques. The lack of key technologies for indoor propagation of the black hooked hesperid butterfly has become a technical bottleneck for fundamental research on its biology, ecology, and toxicology. Therefore, establishing a standardized and standardized indoor propagation technique for the black hooked hesperid butterfly, thereby providing a stable, consistent, and reliable source for ongoing research on the butterfly and providing technical support for its basic biology and integrated control, is a pressing technical challenge. Furthermore, the large numbers of adults reared on a large scale can be further developed and utilized as butterfly specimens and a valuable resource for butterfly gardens, transforming this pest into a valuable asset. Summary of the Invention

[0006] The object of the present invention is to overcome at least one disadvantage of the prior art and provide an indoor propagation method for the black hooked butterfly.

[0007] The technical solution adopted by the present invention is:

[0008] The present invention provides an indoor propagation method of black hooked butterfly, comprising the following steps:

[0009] 1) Using Amomum villosum leaves as the oviposition substrate, placing them in an insect cage supplemented with exogenous nutrient solution, inserting their petioles into a traditional Chinese medicine extract, and introducing adult black hooked hesperids for mating and oviposition;

[0010] 2) Collecting oviposition substrates carrying black hooked butterfly eggs;

[0011] 3) placing the egg-laying matrix carrying the black hook butterfly eggs in an insect box containing an antibacterial and moisturizing matrix at the bottom for incubation and larval culture, collecting the pupae when they emerge, and raising them as adults, wherein the antibacterial and moisturizing matrix is ​​adsorbed with the traditional Chinese medicine extract;

[0012] The active ingredients of the traditional Chinese medicine extract are water extracts of Daibaijie, Gynostemma pentaphyllum and Shencha.

[0013] In some examples, the mass ratio of Gynostemma pentaphyllum, Daibaijie and Shencha in the traditional Chinese medicine extract is (1-5): (1-5): (1-5).

[0014] In some examples, the preparation method of the traditional Chinese medicine extract is: mix Gynostemma pentaphyllum, Daibaijie and Shencha, soak them in water and then boil them, filter the obtained decoction, and dilute it with water to a mass percentage of 4.5% to 5.5% to obtain the traditional Chinese medicine extract.

[0015] In some examples, the mass ratio of the exogenous supplementary nutrient solution in step 1 is: 7.5% to 12% carbohydrates, and the balance is water.

[0016] In some examples, the sugar substance is selected from at least one of sucrose, glucose, fructose, and maltose.

[0017] In some examples, the female-to-male ratio of the black hooked butterfly adults in step 1 is (1-3):1.

[0018] In some examples, the mass composition of the antibacterial moisturizing matrix in step 3 is: 0.5% to 1.5% gel material, 1.5% to 2.5% Chinese herbal extract, and 96% to 98% water.

[0019] In some examples, the insect cages and insect boxes in steps 1 and 3 are placed at a temperature of 24 to 29°C.

[0020] In some examples, the insect cages and insect boxes in steps 1 and 3 are placed in a humidity condition of 70%±5%.

[0021] In some examples, the insect cages and insect boxes in steps 1 and 3 are placed under conditions where the daily light duration is 11 to 13 hours.

[0022] The beneficial effects of the present invention are:

[0023] The present invention sets the host plant for breeding black hooked hesperi and the egg-laying carrier of the black hooked hesperi as Amomum villosum leaves, simplifies the breeding steps, and is conducive to the standardized breeding and expansion of the black hooked hesperi.

[0024] The invention adds a traditional Chinese medicine liquid made from three Dai medicines, namely, Dai Baijie, Jiaogulan and Shencha, which can reduce the fungal infection rate by 28.7%; the antibacterial and moisturizing matrix and the disinfection and moisturizing treatment of Amomum villosum leaves can extend the fresh-keeping time of the leaves from 2 days to about 12 days; newly emerged adults are mated first and then placed in an egg-laying device, which can significantly increase the hatching rate of eggs to more than 80%, and the adult collection rate is more than 75%.

[0025] The method of the present invention is simple and practical, saves time and labor, improves the survival rate of black hooked hesperids, reduces the frequency of food replacement, reduces fungal infection, avoids the decline of laboratory populations, and can obtain a large number of black hooked hesperids of various stages with consistent insect ages in a short period of time, thereby providing a stable insect source for the laboratory and laying a foundation for theoretical research on the biology, ecology, toxicology and other aspects of the black hooked hesperids. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] Figure 1 The plant of Amomum villosum, a Dai medicine, is a planting base;

[0027] Figure 2These are the adults of the black hooked butterfly reared indoors;

[0028] Figure 3 These are the black hooked butterfly eggs laid on the leaves of the Yangchun sand;

[0029] Figure 4 These are young black hooked butterfly larvae that are reared;

[0030] Figure 5 These are the older black hooked butterfly larvae reared;

[0031] Figure 6 Irregular notches left by the black hooked butterfly larvae feeding on the leaves of Amomum villosum;

[0032] Figure 7 The larvae of the black hooked butterfly, which is used for breeding, damage the leaves of Amomum villosum and form cysts;

[0033] Figure 8 The mature larvae of the black hooked butterfly were spinning silk and pupating;

[0034] Figure 9 Black hooked butterfly pupae harvested for breeding;

[0035] Figure 10 The male adult of the reared black hooked butterfly;

[0036] Figure 11 This is a female adult of the reared black hooked butterfly. DETAILED DESCRIPTION

[0037] A method for indoor propagation of black hooked butterfly comprises the following steps:

[0038] 1) Using Amomum villosum leaves as the oviposition substrate, placing them in an insect cage supplemented with exogenous nutrient solution, inserting their petioles into a traditional Chinese medicine extract, and introducing adult black hooked hesperids for mating and oviposition;

[0039] 2) Collecting oviposition substrates carrying black hooked butterfly eggs;

[0040] 3) placing the egg-laying matrix carrying the black hook butterfly eggs in an insect box containing an antibacterial and moisturizing matrix at the bottom for incubation and larval culture, collecting the pupae when they emerge, and raising them as adults, wherein the antibacterial and moisturizing matrix is ​​adsorbed with the traditional Chinese medicine extract;

[0041] The active ingredients of the traditional Chinese medicine extract are water extracts of Daibaijie, Gynostemma pentaphyllum and Shencha.

[0042] In order to reduce the infection rate, the leaves of Amomum villosum should be disinfected before use, such as wiping their surface with a residue-free component such as alcohol.

[0043] In some examples, the mass ratio of Gynostemma pentaphyllum, Daibaijie, and Shencha in the Chinese herbal medicine extract is (1-5):(1-5):(1-5). Preferably, the mass ratio of Gynostemma pentaphyllum, Daibaijie, and Shencha is 1:1:1.

[0044] In some examples, the preparation method of the traditional Chinese medicine extract is: mix Gynostemma pentaphyllum, Daibaijie and Shencha, soak them in water and then boil them, filter the obtained decoction, and dilute it with water to a mass percentage of 4.5% to 5.5% to obtain the traditional Chinese medicine extract.

[0045] The role of the exogenous supplementary nutrient solution is to provide the necessary energy for the black hooked butterfly adults. The nutrient solution commonly used in this field can be used. In some examples, the mass ratio of the exogenous supplementary nutrient solution in step 1 is: 7.5% to 12% carbohydrates, and the balance is water.

[0046] In some embodiments, the sugar is selected from at least one of sucrose, glucose, fructose, and maltose. These monosaccharides or disaccharides are more likely to be utilized by the black hooked skipper.

[0047] In some examples, the female-to-male ratio of the black hooked butterfly adults in step 1 is (1-3):1.

[0048] In some examples, the mass composition of the antibacterial moisturizing matrix in step 3 is: 0.5% to 1.5% gel material, 1.5% to 2.5% Chinese herbal extract, and 96% to 98% water.

[0049] In some examples, the insect cages and insect boxes in steps 1 and 3 are placed at a temperature of 24 to 29°C.

[0050] In some examples, the insect cages and insect boxes in steps 1 and 3 are placed in a humidity condition of 70%±5%.

[0051] In some examples, the insect cages and insect boxes in steps 1 and 3 are placed under conditions where the daily light duration is 11 to 13 hours.

[0052] The following is a further description of specific embodiments of the present invention. It should be noted that the description of these embodiments is intended to facilitate understanding of the present invention and does not constitute a limitation of the present invention. In addition, the technical features involved in the various embodiments of the present invention described below may be combined with each other as long as they do not conflict with each other.

[0053] The experimental methods in the following examples are conventional methods unless otherwise specified, and the experimental materials used in the following examples are commercially available unless otherwise specified.

[0054] The centrifuge tubes, insect boxes, insect cages, insect collection cages, culture dishes, tweezers and other related tools used in the examples were cleaned, disinfected with 75% alcohol, and dried before use.

[0055] Example 1

[0056] 1. Obtaining Yangchunsha

[0057] Host plant Amomum villosum Amomum villosum Lour The leaves were collected from the Amomum villosum cultivation base of the Institute of Medicinal Plants, Chinese Academy of Medical Sciences, Yunnan Branch, Jinghong City, Yunnan Province. The Amomum villosum is cultivated and managed normally by a dedicated person throughout its growth period without the use of any pesticides. The Amomum villosum plants at the base (such as Figure 1 as shown) can provide host plant support for the experiment.

[0058] 2. Preparation of Chinese medicine solution

[0059] Take the preparation of 140mL Chinese medicine solution as an example: weigh Gynostemma pentaphyllum ( Gynostemmapentaphyllum )、Dai Baijie( Marsdeniatenacissima ) and Kidney Tea[ Clerodendranthus spicatus (Thunb.) CY Wu] Place 10 g of each of the three Dai herbs in a pot, add 1000 mL of water, soak for 40 minutes, and then boil. First, boil over high heat for 20 minutes, then simmer over low heat for 20 minutes to obtain a decoction. Finally, filter through gauze to remove the residue, resulting in 120 mL of the traditional Chinese medicine solution. After cooling, refrigerate and store until ready for use.

[0060] The preparation method of the 5% Chinese medicine solution used subsequently is as follows: add distilled water to the above Chinese medicine solution at a standard ratio of 5:95 by weight of Chinese medicine solution: distilled water to obtain a 5% Chinese medicine solution.

[0061] 3. Preparation of antibacterial and moisturizing matrix and insect breeding box

[0062] Weigh 1.0 g of agar, 2.0 g of Chinese herbal medicine liquid, and 97 mL of water respectively. Boil the water first, then add the agar. After it is fully melted, add the Chinese herbal medicine liquid. Then add it to the insect box or culture dish while it is hot, and spread it flat on the bottom of the insect box or culture dish to a thickness of 1.0 cm. After it solidifies and cools, it becomes an antibacterial and moisturizing matrix.

[0063] Unless otherwise specified, the hatching boxes, insect raising boxes, and larval rearing boxes described below are all plastic boxes with an antibacterial and moisturizing matrix prepared on the bottom.

[0064] 4. Egg collection

[0065] (1) Preparation of egg-laying matrix: The egg-laying matrix is ​​fresh, pest-free Amomum villosum leaves from the Amomum villosum planting base. Place 20 Amomum villosum leaves in 5L of clean water for cleaning, then rinse under tap water for 20s. Then use a spray bottle filled with 75% alcohol to evenly spray the front and back of the leaves for disinfection. Finally, lay the disinfected leaves one by one on filter paper and use absorbent paper towels to wipe off the water droplets on the leaves for later use. When using, insert the petiole of the Amomum villosum leaf into a 2mL plastic centrifuge tube filled with 5% Chinese medicine solution. The petiole is fixed with absorbent cotton and the mouth of the centrifuge tube is sealed with absorbent cotton.

[0066] (2) Setting up the egg-laying device: The egg-laying device is a rectangular insect cage with a length, width and height of 75.0 cm × 50.0 cm × 50.0 cm. A rectangular zipper door with a length of 50 cm and a height of 40 cm is set in the center of one side. There is a sleeve (50 cm long and 20 cm in diameter) on the opening surface to facilitate the placement of samples and experimental operations. In each egg-laying device, a leaf of Amomum villosum inserted into a plastic centrifuge tube filled with 5% Chinese medicine solution is placed.

[0067] (3) Egg laying: Collect adult black hooked slugs from the Yangchunsha plantation and transfer no more than 20 adults to the rearing cages using a collecting cage. Set the male-female ratio of the adults to 2:1. Leave the cages for 6 hours to allow the adults to mate. Then, use the collecting cages to transfer no more than 20 mated black hooked slugs to each egg laying device using a collecting cage. Set the male-female ratio to 2:1. Close the zipper door and tie the sleeves tightly for rearing. The temperature is 24-29℃, the humidity is 70%±5%, and the photoperiod is 12L:12D.

[0068] (4) Egg collection: Collect the leaves of Amomum villosum carrying black hooked butterfly eggs every day and add new egg-laying substrate. The black hooked butterfly adults will actively move to the new substrate. After collecting, add the same amount of fresh Amomum villosum leaves.

[0069] 5. Egg hatching and larval rearing

[0070] Black hooked hesperid incubation boxes were set up. Both the incubation boxes and larval rearing boxes were identical plastic boxes (10.0 cm × 7.0 cm × 5.0 cm in length, width, and height, with a 5 cm long, 2 cm wide rectangular opening in the center of the lid, covered with 200-mesh gauze). Eggs typically hatch around 6 days after being laid. Each incubation box contained a leaf of Amomum villosum carrying a black hooked hesperid egg. The petiole of this leaf was inserted into a 2 mL plastic centrifuge tube filled with 5% traditional Chinese medicine solution. The petiole was secured with absorbent cotton, and the tube mouth was sealed with absorbent cotton. The centrifuge tube was then placed upside down in the larval rearing box containing an antibacterial and moisturizing matrix at the bottom and the lid was secured. Starting from the 7th day, Amomum villosum leaves were added according to feeding conditions. The petioles of these leaves were also inserted into 2 mL plastic centrifuge tubes filled with 5% Chinese medicine solution and fixed with absorbent cotton. Larvae were reared at a temperature of 24-90°C, a humidity of 70%±5%, and a photoperiod of 12L:12D.

[0071] 6. Collection of pupae

[0072] Observe the pupation of mature black hooked hesperid larvae in the larval rearing box daily. If pupation is detected, collect it in a 9 cm diameter petri dish containing an antibacterial moisture-retaining matrix. Place the dish open in the adult rearing cage.

[0073] 7. Adult rearing and collection

[0074] Observe the emergence of black hooked hesperid pupae in the culture dishes within the cages daily. Place cotton balls dipped in 10% mixed nutrient solution inside the cage lids to feed the adults. Keep the number of adults in the adult rearing device (the same cages as the egg-laying device) below 20 (with no fewer than 7 males). Then, use a collection cage to capture the specified number of adult black hooked hesperids and transfer them to the newly installed egg-laying device for continued egg-laying.

[0075] 8. Low temperature preservation of pupae

[0076] A selection of pupae of the black hooked hessian butterfly was collected and preserved to rationally manage the indoor population and to allow for breeding when large experimental needs arose. This provided a stable and abundant source of insects of uniform age for experimental purposes. The pupae were placed in plastic boxes (30 × 20 × 10 cm in length × width × height) with an antibacterial, moisturizing matrix at the bottom. Each box contained 20-30 pupae, which were then placed in an artificial climate chamber for low-temperature storage. The low-temperature storage conditions and shelf life of black hooked hessian butterfly pupae are: 16°C-20°C for 21 days, ensuring a collection rate of at least 75% for adult insects.

[0077] Comparative Example 1

[0078] The cultivation method and conditions were the same as those in Example 1, except that the centrifuge tubes were not treated in any way during the egg hatching and larval rearing in step 5.

[0079] Comparative Example 2

[0080] The cultivation method and conditions are the same as those in Example 1, except that only water is added to the centrifuge tube during the egg hatching and larval rearing in step 5, without adding the Chinese medicine solution.

[0081] Comparative Example 3

[0082] The cultivation method and conditions are the same as those in Example 1, except that the composition of the Chinese medicinal liquid in the centrifuge tube during the egg hatching and larval rearing in step 5 is Dai Baijie, Gynostemma pentaphyllum and water.

[0083] Comparative Example 4

[0084] The cultivation method and conditions are the same as those in Example 1, except that no antibacterial moisturizing matrix is ​​added to the rearing box during the egg hatching and larval rearing in step 5.

[0085] Comparative Example 5

[0086] The cultivation method and conditions were the same as those in Example 1, except that only wet filter paper was added to the rearing box during the egg hatching and larval rearing in step 5.

[0087] Comparative Example 6

[0088] The cultivation method and conditions were the same as those in Example 1, except that only agar was added to the rearing box during the hatching of the eggs and larval rearing in step 5.

[0089] Comparative Example 7

[0090] The cultivation method and conditions are the same as those in Example 1, except that in step 5, the composition of the antibacterial and moisturizing matrix of the Chinese medicinal liquid in the breeding box during egg hatching and larval rearing is Dai Baijie, Gynostemma pentaphyllum and water.

[0091] Then, the above embodiments and comparative examples were tested on the effect of adding Chinese medicine liquid on the shelf life of Amomum villosum leaves (20 repetitions), the effect of antibacterial and moisturizing matrix on the infection rate of fungal diseases of black hooked butterfly (10 repetitions), observation of the developmental period of black hooked butterfly (10 repetitions), observation of the life span of black hooked butterfly adults (10 repetitions), the collection rate of black hooked butterfly adults under different temperature storage conditions (10 repetitions), the growth and decline pattern of black hooked butterfly adult population (10 repetitions), and the daily activity pattern of black hooked butterfly adults (10 repetitions). The results are shown in Tables 1 to 7.

[0092] Table 1 Effects of adding Chinese medicinal liquid on the shelf life of Amomum villosum leaves

[0093] Treatment group Average shelf life (d) Example (centrifuge tube + water + Chinese medicine solution) 12.2 Comparative Example 1 (empty centrifuge tube without treatment) 1.6 Comparative Example 2 (centrifuge tube + water) 7.4 Comparative Example 3 [centrifuge tube + water + Chinese medicine solution (the Chinese medicine extract consists of Dai Baijie, Gynostemma pentaphyllum and water)] 9.3

[0094] Table 2 Effects of antibacterial and moisturizing matrix on the infection rate of fungal diseases of the black hooked butterfly

[0095] Treatment group Black hooked butterfly disease infection rate (%) Example (rearing box + agar + Chinese medicine solution) 3.5 Comparative Example 4 (empty breeding box without treatment) 32.2 Comparative Example 5 (breeding box + wet filter paper) 34.0 Comparative Example 6 (rearing box + agar) 31.4 Comparative Example 7 [rearing box + agar + Chinese medicine solution (the Chinese medicine extract consists of Dai Baijie, Gynostemma pentaphyllum and water)] 7.6

[0096] Table 3 Developmental period of the black hooked butterfly (days)

[0097] insect stage Day (d) Egg stage 5.40 larval stage 22.32 pupal stage 9.46 Pre-spawning period 2.14 Generational period 39.32

[0098] Table 4 Lifespan of black hooked butterfly adults

[0099] Treatment group Average lifespan of adults (d) 1 10 2 15 3 14 4 12 5 13 6 14 7 15 8 16 9 17 10 15

[0100] Table 5 Collection rate of adults of Black Hooked Skipper under different storage temperature conditions

[0101] Low temperature storage conditions Average collection rate of adults (%) 16℃ / 14d 83.2 16℃ / 21d 75.4 20℃ / 14d 87.8 20℃ / 21d 82.0

[0102] Table 6 Population fluctuation patterns of adult black hooked butterfly (Xishuangbanna, Yunnan)

[0103] month Number of adults (heads) 1 0 2 0 3 3 4 6 5 9 6 14 7 16 8 11 9 8 10 5 11 7 12 4

[0104] Table 7 Daily activity patterns of adults of the black hooked butterfly (June to July in Xishuangbanna, Yunnan)

[0105] Time period Number of adults (heads) 7:00-8:00 1 9:00-10:00 8 11:00-12:00 5 13:00-14:00 1 15:00-16:00 4 17:00-18:00 2

[0106] As shown in Table 1, the treatment with a traditional Chinese medicine solution significantly extended the shelf life of Amomum villosum leaves to 12 days. As shown in Table 2, the treatment with an antibacterial and moisturizing matrix effectively reduced the infection rate of fungal diseases in the black hooked hesperid by 28.7%. Table 3 shows that the generation period of the black hooked hesperid is 39.32 days, with the larval stage accounting for 56.8% of the entire generation. Table 4 shows that the adult lifespan of the black hooked hesperid is 14 days. Table 5 shows that after 21 days of low-temperature storage at 16°C-20°C, the adult recovery rate can reach over 75%.

[0107] Table 6 shows that the adult population of this insect in Banna begins to increase in March, peaks in June and July, and fluctuates in November. Table 7 shows that the daily activity peak of this insect occurs primarily between 9:00 and 12:00 in the morning, with some fluctuations between 15:00 and 16:00. Its population is relatively low during the rest of the day.

[0108] The embodiments of the present invention are described in detail above, but the present invention is not limited to the described embodiments. It is apparent to those skilled in the art that various changes, modifications, substitutions, and variations of these embodiments may be made without departing from the principles and spirit of the present invention, and the changes still fall within the scope of protection of the present invention.

Claims

1. A method for indoor propagation of black hooked butterfly, characterized in that: The following steps are involved: 1) Using Amomum villosum leaves as the oviposition substrate, the leaves were placed in an insect cage supplemented with exogenous nutrient solution, the petioles were inserted into a traditional Chinese medicine extract, and adult black hooked hesperids were introduced for mating and oviposition; 2) Collecting oviposition substrates carrying black hooked butterfly eggs; 3) placing the egg-laying matrix carrying black hooked butterfly eggs in an insect box containing an antibacterial and moisturizing matrix at the bottom for incubation and larval culture, collecting the pupae when they emerge, and raising them as adults, wherein the antibacterial and moisturizing matrix is ​​adsorbed with the traditional Chinese medicine extract, and the mass composition of the antibacterial and moisturizing matrix is: 0.5% to 1.5% gel material, 1.5% to 2.5% traditional Chinese medicine extract, and 96% to 98% water; The active ingredients of the Chinese medicine extract are water extracts of Dai Baijie, Gynostemma pentaphyllum, and Shencha, and the mass ratio of Gynostemma pentaphyllum, Dai Baijie, and Shencha in the Chinese medicine extract is (1-5):(1-5):(1-5). The preparation method of the Chinese medicine extract is: mixing Gynostemma pentaphyllum, Dai Baijie, and Shencha, soaking them in water, and then decocting them. After filtering the obtained decoction, the decoction is diluted with water to a mass percentage of 4.5% to 5.5%, thereby obtaining the Chinese medicine extract.

2. The indoor propagation method of the black hooked butterfly according to claim 1, characterized in that: The mass ratio of the exogenous supplementary nutrient solution in step 1 is: 7.5% to 12% carbohydrates, and the balance is water.

3. The indoor propagation method of the black hooked butterfly according to claim 2, characterized in that: The sugar substance is selected from at least one of sucrose, glucose, fructose and maltose.

4. The indoor propagation method of the black hooked butterfly according to claim 1, characterized in that: In the step 1, the male-female ratio of the black hooked butterfly adults is (1-3):

1.

5. The indoor propagation method of the black hooked butterfly according to claim 1, characterized in that: In the steps 1 and 3, the insect cages and insect boxes are placed at a temperature of 24 to 29° C. for breeding.

6. The indoor propagation method of the black hooked butterfly according to claim 1, characterized in that: In steps 1 and 3, the insect cages and insect boxes are placed in a humidity condition of 70% ± 5%.

7. The indoor propagation method of the black hooked butterfly according to claim 1, characterized in that: The insect cages and insect boxes in steps 1 and 3 are placed under conditions where the daily light duration is 11 to 13 hours.

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