Electrode, collection method and preparation method for collecting electromyographic electricity of masticatory muscles in awake mice

Abstract

The present invention discloses an electrode for collecting electromyography of masticatory muscles in the awake state of mice, a collection method and a preparation method, wherein the electrode for collecting electromyography of masticatory muscles in the awake state of mice comprises a base for fixing on the top of the mouse skull; a female connector is provided on the base, a female connector hole is provided in the female connector, a plurality of welding lugs are provided on the outer peripheral surface of the female connector, one end of each welding lug is electrically connected to the female connector, and the other end is connected to a flexible electrode line, the end of the flexible electrode line is connected to a recording probe, and the end of the recording probe has a barb. The electrode for collecting electromyography of masticatory muscles in the awake state of mice of the invention can collect electromyography of masticatory muscles in the awake state of mice, thereby providing more accurate and comprehensive data for research.

Description

Technical Field

[0001] The invention belongs to the technical field of electromyographic acquisition of masticatory muscles of experimental small animals, and in particular relates to an electromyographic acquisition electrode for masticatory muscles of awake mice, an acquisition method and a preparation method. Background Art

[0002] Temporomandibular disorder (TMD) is a general term for a class of diseases involving the temporomandibular joint and masticatory muscles. Its incidence rate is about 18%-54%, ranking fourth in oral diseases after periodontal disease, caries, and malocclusion. Masticatory muscle dysfunction is one of the important classifications of TMD. The main symptoms are myofascial pain, oromandibular muscle spasm, parafunctional movements (bruxism), and even limited mouth opening, which seriously affect the patient's quality of life. The etiology of masticatory muscle dysfunction is complex. Studies have confirmed that it is closely related to factors such as abnormal occlusion, mental psychology, and systemic endocrine changes. Therefore, clinical and basic research on temporomandibular joint and masticatory muscle dysfunction has always been one of the hot topics in oral medicine research.

[0003] Among the technical means of studying the physiological and pathological changes of masticatory muscles, the electromyographic activity level of masticatory muscles is one of the important indicators for judging the severity of the disease and the prognosis. There are currently two methods for measuring the electromyography of masticatory muscles. One is surface electromyography measurement, which is to attach electrodes to the skin to derive the activity potential of the more superficial masticatory muscles (masseter muscles and temporalis muscles); the other is needle electrode measurement, which is to insert the electrode needle into the muscle to be measured and derive the local potential. However, in the process of animal experiments, both measurement methods can only be completed under anesthesia due to the inability of animals to cooperate, and cannot well simulate the changes in muscle activity of humans in the awake state, thus limiting the development of many related animal experiments. Summary of the invention

[0004] In response to the problems existing in the prior art, the present invention provides an electrode for collecting electromyography of the masticatory muscles of mice in an awake state, which has the advantage of being able to collect electromyography of the masticatory muscles of mice in an awake state, thereby providing more accurate and comprehensive data for research. It solves the problem that existing animals are unable to cooperate and can only complete measurements under anesthesia, and cannot well simulate the changes in muscle activity of humans in an awake state, thus limiting the development of many related animal experiments.

[0005] The present invention is implemented in this way. The present invention provides an electrode for collecting electromyographic electricity of the masticatory muscles of mice in a waking state, comprising:

[0006] A base for fixing to the top of the mouse skull;

[0007] A female connector is provided on the base, a female connection hole is provided in the female connector, a plurality of welding protrusions are provided on the outer peripheral surface of the female connector, one end of each welding protrusion is electrically connected to the female connector, and the other end is connected to a flexible electrode wire, the end of the flexible electrode wire is connected to a recording probe, and the end of the recording probe has a barb.

[0008] As a preferred embodiment of the present invention, the bottom surface of the base is a concave curved surface structure matching the contact surface of the mouse skull, and a perfusion port penetrating the base is provided on the base located at the periphery of the female connector. When the base is set on the mouse skull, dental cement is injected into the perfusion port to fix the base on the mouse skull;

[0009] The said filling ports include a plurality of filling ports, and the filling ports are arranged in a circular array on the said base;

[0010] The cross-sectional shape of the injection port is composed of a circular hole and a notch connected to the circular hole, the circular hole penetrates the upper and lower end surfaces of the base, and the notch penetrates the upper and lower end surfaces and the outer peripheral surface of the base.

[0011] As a preferred embodiment of the present invention, the base and the female connector are both cylinders, and the base and the female connector are connected up and down to form a stepped column structure, and the outer diameter of the female connector is smaller than the outer diameter of the base.

[0012] As a preferred embodiment of the present invention, the cross-section of the flexible electrode wire is elliptical;

[0013] The flexible electrode wire comprises an insulating skin layer and a conductive core layer, and the insulating skin layer wraps the conductive core layer.

[0014] As a preferred embodiment of the present invention, the end of the flexible electrode wire and the tail of the recording probe are welded or detachably connected;

[0015] Wherein, the configuration of the detachable connection between the end of the flexible electrode line and the tail of the recording probe comprises:

[0016] The tail of the recording probe is fixedly connected with a first connecting piece, one side of the first connecting piece is a first arc-shaped surface; the other side is a first plane, and the first plane has a second slot; the end of the flexible electrode line is fixedly connected with a second connecting piece, one side of the second connecting piece is a second arc-shaped surface, and the other side is a second plane, and the second plane has a card block that matches the second slot; after the first plane and the second plane are attached, the second slot is inserted into the card block, and the first connecting piece and the second connecting piece form a cylindrical structure; the surface of the flexible electrode line is sleeved with an elastic sleeve, and the elastic sleeve is sleeved on the outside of the first connecting piece and the second connecting piece;

[0017] The second card slot is an inclined block, the card block is an inclined slot, and the inner surface of the elastic sleeve is in contact with the outer surfaces of the first connecting piece and the second connecting piece;

[0018] An annular groove is fixedly connected to the recording probe, and an elastic ring is provided on the inner surface of the elastic sleeve, and the elastic ring can be snapped into the annular groove.

[0019] As a preferred embodiment of the present invention, the end of the recording probe is a cone, the number of the barbs is at least 2, and the barbs are arranged on the circumferential surface of the recording probe.

[0020] As a preferred embodiment of the present invention, it includes a plurality of flexible electrode wires and a plurality of recording probes, and at least some of the recording probes and / or at least some of the flexible electrode wires have different sizes, so as to simultaneously detect the four masticatory muscles, namely the masseter muscle, the temporalis muscle, the medial pterygoid muscle and the lateral pterygoid muscle.

[0021] The method for collecting electromyographic information of the masticatory muscles of mice in a waking state comprises the following steps:

[0022] Anesthetize the mouse and expose the parietal bones and masticatory muscles of the mouse;

[0023] inserting a recording probe of an electromyographic electrode deep into the masticatory muscle;

[0024] The base of the electromyographic electrode is fixed on the parietal bone of the mouse, and the curved surface structure at the lower end of the base matches the surface of the parietal bone of the mouse;

[0025] The flexible electrode wire connected to the recording probe is buried in the subcutaneous tissue between the parietal bone and the masticatory muscle area of ​​the mouse, and the flexible electrode wire is fixed by suturing at least one place;

[0026] Connect the female connector to the recording end of the recorder. After the mouse has healed for a preset period of time, collect the electromyography of the masticatory muscles while the mouse is awake, and generate an electromyogram based on the signals from the electromyographic electrodes.

[0027] The method for preparing an electrode for collecting electromyographic electricity from the masticatory muscles of mice in a waking state comprises the following steps:

[0028] Make flexible electrode wires, bases, recording probes, and female connectors;

[0029] A barb is provided at the tip of the recording probe, the tail of the recording probe is fixedly connected to the end of the flexible electrode line, the other end of the flexible electrode line is connected to the female connector, and the female connector is connected to the base.

[0030] As a preferred embodiment of the present invention, the method for making a flexible electrode wire includes:

[0031] The conductive core layer and the metal wire are arranged side by side at equal distances, and both ends of the metal wire are shorter than the conductive core layer;

[0032] Wrapping the conductive core layer and the metal wire with an insulating layer;

[0033] The insulating skin layer is drilled until the metal wire is exposed, the metal wire is pulled out of the hole, and an air extraction and release mechanism is connected at the hole to obtain a flexible electrode wire with an airway.

[0034] Compared with the prior art, the present invention has the following beneficial effects:

[0035] 1. In the present invention, the masticatory muscle electromyography acquisition electrode for the awake state of mice is provided with a base, a female connector, a flexible electrode line and a recording probe; when used specifically, the mouse is anesthetized first, and the mouse skull and masticatory muscle are exposed; the recording probe of the electromyography electrode is inserted into the deep part of the masticatory muscle area, wherein the barb is used to hook the mouse masticatory muscle, and the recording probe is fixed in the masticatory muscle area; the base of the electromyography electrode is fixed to the mouse skull, and the curved surface structure at the lower end of the base matches the surface of the mouse skull; the flexible electrode line connected to the recording probe is buried in the subcutaneous tissue between the mouse skull and the masticatory muscle area, and the flexible electrode line is fixed by suturing at least one place; the female connector is connected to the recording end of the recorder, and after the mouse has healed for a preset time, the masticatory muscle electromyography is collected in the awake state of the mouse, and an electromyogram is generated according to the signal from the electromyography electrode. Through the above-mentioned setting, the masticatory muscle electromyography of the mouse can be collected in the awake state, and the obtained data is more accurate and comprehensive. Through this device, the masticatory muscle electromyography of the mouse in the awake state can be collected, thereby providing more accurate and comprehensive data for research. BRIEF DESCRIPTION OF THE DRAWINGS

[0036] Figure 1 It is a schematic diagram of the three-dimensional structure of the electromyographic acquisition electrode for the masticatory muscles of mice in an awake state provided by an embodiment of the present invention;

[0037] Figure 2 The embodiment of the present invention provides Figure 1 A schematic diagram of the enlarged structure of part A;

[0038] Figure 3 1 is a schematic diagram of a top view of an electrode for collecting electromyographic electromyography of a masticatory muscle in an awake state of a mouse provided in an embodiment of the present invention;

[0039] Figure 4 The embodiment of the present invention provides Figure 3 A schematic diagram of the enlarged structure of part B;

[0040] Figure 5 is a schematic diagram of the cross-sectional structure of a flexible electrode wire provided by an embodiment of the present invention;

[0041] Figure 6is a schematic diagram of the three-dimensional structure of the connection between the flexible electrode wire and the recording probe provided by an embodiment of the present invention;

[0042] Figure 7 It is a schematic diagram of a structure in which the elastic sleeve is omitted at the connection between the flexible electrode wire and the recording probe provided by an embodiment of the present invention;

[0043] Figure 8 The embodiment of the present invention provides Figure 7 A schematic diagram of the enlarged structure of the middle C part;

[0044] Fig. 9 is a schematic cross-sectional view of the connection between the flexible electrode wire and the recording probe provided by an embodiment of the present invention;

[0045] Fig.10 The embodiment of the present invention provides Fig. 9 A schematic diagram of the enlarged structure of the D part;

[0046] Fig.11 is a schematic side view of the structure of a first connecting piece and a second connecting piece provided in an embodiment of the present invention;

[0047] Fig.12 It is a flowchart of a method for collecting electromyographic electricity from the masticatory muscles of mice in an awake state provided by an embodiment of the present invention;

[0048] Fig.13 It is a flowchart of a method for preparing an electrode for collecting electromyographic electricity from the masticatory muscles of mice in an awake state provided by an embodiment of the present invention;

[0049] Fig.14 It is a flowchart of a method for manufacturing a flexible electrode line provided by an embodiment of the present invention.

[0050] In the figure: 100, base; 200, female connector; 210, female connector hole; 220, welding lug; 300, flexible electrode wire; 400, recording probe; 401, barb;

[0051] 110, filling port; 111, round hole; 112, notch; 113, first card slot;

[0052] 310, insulating skin layer; 320, conductive core layer; 311, airway;

[0053] 410, first connecting piece; 411, first arcuate surface; 412, first plane; 413, second slot; 330, second connecting piece; 331, second arcuate surface; 332, second plane; 333, block; 340, elastic sleeve; 420, annular groove; 341, elastic ring. DETAILED DESCRIPTION

[0054] In order to further understand the content, features and effects of the present invention, the following embodiments are given as examples and described in detail with reference to the accompanying drawings.

[0055] The structure of the present invention is described in detail below in conjunction with the accompanying drawings.

[0056] The present invention provides an electrode for collecting electromyographic electricity from the masticatory muscles of mice in an awake state, including the following embodiments:

[0057] Example 1

[0058] See also Figure 1 and Figure 2 The embodiment of the present invention provides an electrode for collecting electromyographic electricity of the masticatory muscles of mice in an awake state, comprising:

[0059] A base 100 for fixing to the top of the mouse skull;

[0060] The base 100 is provided with a female connector 200, a female connection hole 210 is provided inside the female connector 200, a plurality of welding protrusions 220 are provided on the outer peripheral surface of the female connector 200, one end of each welding protrusion 220 is electrically connected to the female connector 200, and the other end is connected to a flexible electrode line 300, the end of the flexible electrode line 300 is connected to a recording probe 400, and the end of the recording probe 400 has a barb 401.

[0061] The above scheme is adopted: when it is used specifically, the mouse is anesthetized first, and the mouse skull parietal bone and the masticatory muscle area are exposed; the recording probe 400 of the electromyographic electrode is inserted into the deep part of the masticatory muscle area, wherein the barb 401 is used to hook the mouse masticatory muscle, and the recording probe 400 is fixed in the masticatory muscle area; the base 100 of the electromyographic electrode is fixed to the mouse skull parietal bone, and the curved surface structure at the lower end of the base 100 matches the surface of the mouse skull parietal bone; the flexible electrode line 300 connected to the recording probe 400 is buried in the subcutaneous tissue between the mouse skull parietal bone and the masticatory muscle area, and the flexible electrode line 300 is fixed by suturing at least one place; the female connector 200 is connected to the recording end of the recorder, and after the mouse has healed for a preset time, the masticatory muscle electromyography is collected in the awake state of the mouse, and an electromyogram is generated according to the signal from the electromyographic electrode. Through the above settings, the masticatory muscle electromyography of the mouse can be collected in the awake state, and the data obtained are more accurate and comprehensive.

[0062] Through the above device, the electromyographic data of the chewing muscles of mice in the awake state can be collected, thus providing more accurate and comprehensive data for the research.

[0063] It should be noted that the masticatory muscles include the masseter muscle, temporalis muscle, medial pterygoid muscle, and lateral pterygoid muscle, and this embodiment may also detect any one of them separately, such as the masseter muscle, which is within the protection scope of this embodiment. This also applies to the following description and will not be repeated below.

[0064] Example 2

[0065] On the basis of the above embodiment, the following settings are also performed:

[0066] See also Figure 3 and Figure 4 The bottom surface of the base 100 is a concave curved surface structure that matches the contact surface of the mouse skull. A perfusion port 110 that passes through the base 100 is provided on the base 100 located at the periphery of the female connector 200. When the base 100 is set on the mouse skull, dental cement is injected into the perfusion port 110 to fix the base 100 to the mouse skull.

[0067] In this embodiment, the bottom surface of the base 100 is a concave curved surface structure that matches the contact surface of the mouse skull, so that the base 100 can be installed more firmly.

[0068] In this embodiment, the base 100 is set to be cylindrical, and the injection port 110 includes a plurality of injection ports 110, which are arranged in a circumferential array on the base 100. The base 100 and the female connector 200 are both cylindrical, and the base 100 and the female connector 200 are connected up and down to form a stepped column structure, and the outer diameter of the female connector 200 is smaller than the outer diameter of the base 100.

[0069] However, in an optional embodiment, the cross-section of the base 100 is elliptical. In this case, the size of the cutout of the mouse's skull can be smaller, which is beneficial to the recovery of the mouse and improves the experimental efficiency.

[0070] The cross-sectional shape of the perfusion port 110 is formed by connecting a circular hole 111 and a notch 112 connected to the circular hole 111. The circular hole 111 passes through the upper and lower end surfaces of the base 100, and the notch 112 passes through the upper and lower end surfaces and the outer peripheral surface of the base 100. A first card slot 113 is provided at the edge of the perfusion port 110. When the length of the flexible electrode wire 300 is too large, the flexible electrode wire 300 can be clamped in the first card slot 113 to avoid being scattered. In addition, the perfusion port 110 can accommodate dental cement and several folded wires at the same time. Therefore, another use of the perfusion port 110 is that redundant flexible electrode wires 300 can be placed in the perfusion port 110 and fixed by dental cement to avoid being scattered. Alternatively, the flexible electrode wire 300 can be wound between two adjacent perfusion ports 110.

[0071] Example 3

[0072] On the basis of the above embodiment, the following settings are also performed:

[0073] See also Figure 5The flexible electrode line 300 includes an insulating skin layer 310 and a conductive core layer 320 , and the insulating skin layer 310 wraps the conductive core layer 320 .

[0074] For example, the insulating skin layer 310 is a rubber layer, and the conductive core layer 320 is a soft metal wire. On the one hand, this allows the flexible electrode wire 300 to fit more snugly when buried under the skin of a mouse. On the other hand, the place where the insulating skin layer 310 contacts the mouse is insulated, preventing the collected signal from being confused.

[0075] Furthermore, the insulating skin layer 310 has an air channel 311 therein, and the insulating skin layer 310 can be elastically deformed.

[0076] The airway 311 can increase the flexibility of the flexible electrode line 300, so that the flexible electrode line 300 is more docile when buried under the skin of the mouse. Further, the airway 311 is connected to a pumping and deflation mechanism such as a syringe. On the one hand, the pumping and deflation mechanism can inject air into the airway 311, so that the flexible electrode line 300 can become thicker, so that it can be fixed more firmly under the skin of the mouse. On the other hand, the insulating cortex 310 has an airway 311, which can increase the flexibility of the insulating cortex 310. On the other hand, the air is extracted, and the bending degree of the flexible electrode line 300 can be slightly changed. In the case of setting multiple airways 311, some of the airways 311 can be pumped, and some of the airways 311 can be deflated at the same time, that is, the position of the flexible electrode line 300 can be controllably changed in a small amount. When the flexible electrode line 300 is pre-buried, the bending degree of the flexible electrode line 300 can be fine-tuned to make it fit more closely to the subcutaneous tissue, and the position of the recording probe 400 can also be fine-tuned.

[0077] Furthermore, the cross-sectional shape of the flexible electrode wire 300 is an ellipse. On the one hand, the extra part of the ellipse compared to the circle can be used to accommodate the airway 311. On the other hand, it is not easy to rotate, so it is not easy to change the position of the recording probe 400. The rotation of the flexible electrode wire 300 will cause the recording probe 400 to move, resulting in inaccurate detection.

[0078] Furthermore, the outer surface of the insulating layer 310 has an anti-slip member (not shown in the figure), which further makes it difficult to rotate.

[0079] Example 4

[0080] On the basis of the above embodiment, the following settings are also performed:

[0081] See also Figure 6 - Fig.11 The end of the flexible electrode line 300 and the tail of the recording probe 400 are welded or detachably connected.

[0082] The configuration of the detachable connection between the end of the flexible electrode line 300 and the tail of the recording probe 400 includes:

[0083] The tail of the recording probe 400 is fixedly connected with a first connecting piece 410, one side of the first connecting piece 410 is a first arcuate surface 411; the other side is a first plane 412, and the first plane 412 has a second card slot 413; the end of the flexible electrode line 300 is fixedly connected with a second connecting piece 330, one side of the second connecting piece 330 is a second arcuate surface 331, and the other side is a second plane 332, and the second plane 332 has a card block 333 that cooperates with the second card slot 413; after the first plane 412 and the second plane 332 are fitted together, the second card slot 413 is inserted into the card block 333, and the first connecting piece 410 and the second connecting piece 330 form a cylindrical structure; the surface of the flexible electrode line 300 is sleeved with an elastic sleeve 340, and the elastic sleeve 340 is sleeved on the outside of the first connecting piece 410 and the second connecting piece 330.

[0084] Through the above arrangement, the recording probe 400 can be quickly disassembled and assembled, and it is convenient to replace different recording probes 400. And the connection method is simple and firm. Preferably, the second card slot 413 is an inclined block, the card block 333 is an inclined slot, and the inner surface of the elastic sleeve 340 is in contact with the outer surfaces of the first connecting piece 410 and the second connecting piece 330.

[0085] Furthermore, the recording probe 400 is fixedly connected with an annular groove 420, and the inner surface of the elastic sleeve 340 has an elastic ring 341, and the elastic ring 341 can be snapped into the annular groove 420. Through this arrangement, it is not easy to leak electricity.

[0086] Example 5

[0087] On the basis of the above embodiment, the following settings are also performed:

[0088] The end of the recording probe 400 is a cone, the number of the barbs 401 is at least two, and the barbs 401 are arranged on the circumference of the recording probe 400 .

[0089] The barbs 401 can hook onto the masticatory muscles of the mouse, making it easier to fix the recording probe 400 on the masticatory muscles of the mouse.

[0090] It should be noted that the lengths of the barbs 401 are not equal, and / or the barbs 401 are not arranged in a circular array on the recording probe 400 .

[0091] For example, when the muscles are active, long barbs 401 are used in places where the muscles are active or where there are few muscle fiber bundles, and short barbs 401 are used in places where the muscles are active. In places where the muscles are active or where there are few muscle fiber bundles, the number of barbs 401 is large; in places where the muscles are active, the number of barbs 401 is small. In the above manner, the recording probe 400 is not easy to be separated or rotated, and the connection effect is better.

[0092] Furthermore, the connection between the barb 401 and the recording probe 400 has rounded corners, so that the barb 401 is not easy to hook the masticatory muscle and detach. Because the mouse in the present invention is awake, active, and even chewing, it is important to have a firm connection.

[0093] Furthermore, the recording probe 400 is a metal recording probe, and may also be a conductive polymer recording probe.

[0094] For example, the recording probe 400 is made of carbon nano-conductive polymer material, which is soft and can further reduce the impact of the recording probe 400 and the barb 401 on the masticatory muscles.

[0095] Example 6

[0096] On the basis of the above embodiment, the following settings are also performed:

[0097] The method comprises a plurality of flexible electrode wires 300 and a plurality of recording probes 400, and at least some of the recording probes 400 and / or at least some of the flexible electrode wires 300 are of different sizes, and four masticatory muscles, namely, the masseter muscle, the temporalis muscle, the medial pterygoid muscle, and the lateral pterygoid muscle, are detected at the same time.

[0098] The present invention also provides a method for collecting electromyographic information of the masticatory muscles of mice in an awake state, including the following embodiments:

[0099] Example 7

[0100] See also Fig.12 The method for collecting electromyographic information of the masticatory muscles of mice in a waking state comprises the following steps:

[0101] Step S11, anesthetizing the mouse and exposing the parietal bone and masticatory muscle area of ​​the mouse;

[0102] Step S12, inserting the recording probe 400 of the electromyographic electrode into the deep part of the masticatory muscle area;

[0103] Step S13, fixing the base 100 of the electromyographic electrode on the parietal bone of the mouse, so that the curved surface structure at the lower end of the base 100 matches the surface of the parietal bone of the mouse;

[0104] Step S14, burying the flexible electrode wire 300 connected to the recording probe 400 in the subcutaneous tissue between the parietal bone and the masticatory muscle area of ​​the mouse, and fixing the flexible electrode wire 300 by suturing at least one place;

[0105] Step S15, connecting the female connector 200 to the recording end of the recorder, and collecting the electromyography of the masticatory muscles when the mouse is awake after a preset time of healing, and generating an electromyogram based on the signals from the electromyographic electrodes.

[0106] In this way, the electromyographic data of the chewing muscles of mice in the awake state can be collected, thus providing more accurate and comprehensive data for the research.

[0107] Specifically, the step 3 further includes pouring dental cement into the injection port 110 on the base 100 , and the dental cement overflows from the injection port 110 to fix the mouse skull top and the base 100 .

[0108] Example 8

[0109] Five mice were selected from each group of mice for embedding of the flexible electrode wire 300 .

[0110] After intraperitoneal injection of 1% sodium pentobarbital at a dose of 3.5 mg / kg, the mice were anesthetized, and the hair on the left cheek and skull of the mice was shaved, and the above parts were disinfected with iodine. A 1 cm long incision was made at the midpoint of the line connecting the left corner of the mouth and the lower edge of the external ear to expose the left masseter area; a 2 cm long longitudinal incision was made on the top of the skull to expose the skull and scrape the surface periosteum. The tip of the recording probe 400 was inserted into the deep part of the masseter muscle, and the flexible electrode line 300 was run behind the ear and under the skin of the skull, and passed through the incision of the skull, connected to the base 100 of the electromyographic electrode, and the base 100 of the electromyographic electrode was fixed to the mouse skull with dental cement. The wound was cleaned with sterile saline and sutured with silk thread, and the wound was disinfected with iodine again. After the operation, the mouse was placed in a warm environment until it woke up, and the incision of the surgical area was disinfected with iodine every day, and soft food was fed to allow the mouse to recover for 7-10 days. After the incision healed, the restraint stress and other experimental treatments were performed. The masseter EMG of each group of mice was tested one day before the stress and after the restraint stress on the last day of stress. During the test, the female connector on the head of the mouse was connected to the BIOPAC multichannel physiological recorder using an adapter and a cable. The mouse was placed in a container for 30 minutes. After it adapted to the connected cable, the masseter EMG signal of the mouse was collected in the awake state. Each mouse was collected for 30 minutes. All EMG signals were digitized into 200 Hz data using AcqKnowledge software (AcqKnowledge software; Bio PAC, Goleta, CA, USA) and stored in a computer.

[0111] The present invention also provides a method for preparing an electrode for collecting electromyographic electricity from the masticatory muscles of mice in an awake state, including the following embodiments:

[0112] Example 9

[0113] See also Fig.13 The present invention also provides a method for preparing an electrode for collecting electromyographic electricity from the masticatory muscles of mice in an awake state, comprising the following steps:

[0114] Step S21, manufacturing the flexible electrode line 300, the base 100, the recording probe 400, and the female connector 200;

[0115] Step S22, set a barb 401 at the tip of the recording probe 400, fix the tail of the recording probe 400 to the end of the flexible electrode line 300, connect the other end of the flexible electrode line 300 to the female connector 200, and connect the female connector to the base 100.

[0116] Among them, see Fig.14 In step S21, the method for manufacturing the flexible electrode line 300 includes:

[0117] Step S211, arranging the conductive core layer 320 and the metal wire equidistantly and side by side, with both ends of the metal wire shorter than the conductive core layer 320;

[0118] Step S212, wrapping the conductive core layer 320 and the metal wire with the insulating layer 310;

[0119] Step S213, drilling holes in the insulating skin layer 310 until the metal wire is exposed, pulling the metal wire out of the holes, connecting the holes to an air extraction and degassing mechanism, and obtaining a flexible electrode wire 300 with an air channel 311.

[0120] It should be noted that, in this article, relational terms such as first and second, etc. are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Moreover, the terms "include", "comprise" or any other variants thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, article or device.

[0121] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. An electrode for collecting electromyographic electricity from the masticatory muscles of mice in an awake state, characterized in that: include: A base (100) for fixing to the top of the mouse skull; The base (100) is provided with a female connector (200), a female connector hole (210) is provided in the female connector (200), a plurality of welding lugs (220) are provided on the outer peripheral surface of the female connector (200), one end of each welding lug (220) is electrically connected to the female connector (200), and the other end is connected to a flexible electrode line (300), the end of the flexible electrode line (300) is connected to a recording probe (400), and the end of the recording probe (400) has a barb (401); The end of the flexible electrode line (300) and the tail of the recording probe (400) are detachably connected; The arrangement of the detachable connection between the end of the flexible electrode line (300) and the tail of the recording probe (400) comprises: the tail of the recording probe (400) is fixedly connected with a first connecting piece (410), one side of the first connecting piece (410) is a first arc-shaped surface (411); the other side is a first plane (412), and the first plane (412) has a second slot (413); the end of the flexible electrode line (300) is fixedly connected with a second connecting piece (330), one side of the second connecting piece (330) is a second arc-shaped surface (331), and the other side is a first plane (412). One side is a second plane (332), and the second plane (332) has a card block (333) that matches the second card slot (413); after the first plane (412) and the second plane (332) are attached, the second card slot (413) is inserted into the card block (333), and the first connecting piece (410) and the second connecting piece (330) form a cylindrical structure; the surface of the flexible electrode wire (300) is sleeved with an elastic sleeve (340), and the elastic sleeve (340) is sleeved on the outside of the first connecting piece (410) and the second connecting piece (330); The second clamping groove (413) is an inclined block, the clamping block (333) is an inclined groove, and the inner surface of the elastic sleeve (340) is in contact with the outer surfaces of the first connecting piece (410) and the second connecting piece (330); The recording probe (400) is fixedly connected with an annular groove (420), and the inner surface of the elastic sleeve (340) is provided with an elastic ring (341), and the elastic ring (341) can be snapped into the annular groove (420).

2. The electrode for collecting electromyographic signals of the masticatory muscles of mice in an awake state as claimed in claim 1, characterized in that: The bottom surface of the base (100) is a concave curved surface structure matching the contact surface of the mouse skull, and a perfusion port (110) penetrating the base (100) is provided on the base (100) located at the periphery of the female connector (200). When the base (100) is placed on the mouse skull, dental cement is injected into the perfusion port (110) to fix the base (100) on the mouse skull; The pouring ports (110) include a plurality of pouring ports (110), and the pouring ports (110) are arranged in a circular array on the base (100); The cross-sectional shape of the pouring port (110) is composed of a circular hole (111) and a notch (112) connected to the circular hole (111); the circular hole (111) passes through the upper and lower end surfaces of the base (100); and the notch (112) passes through the upper and lower end surfaces and the outer peripheral surface of the base (100).

3. The electrode for collecting electromyographic signals of the masticatory muscles of mice in an awake state as claimed in claim 1, characterized in that: The base (100) and the female connector (200) are both cylindrical, and the base (100) and the female connector (200) are connected up and down to form a stepped columnar structure, and the outer diameter of the female connector (200) is smaller than the outer diameter of the base (100).

4. The electrode for collecting electromyographic signals of the masticatory muscles of mice in an awake state as claimed in claim 1, characterized in that: The cross section of the flexible electrode wire (300) is elliptical; The flexible electrode wire (300) comprises an insulating skin layer (310) and a conductive core layer (320), wherein the insulating skin layer (310) wraps the conductive core layer (320).

5. The electrode for collecting electromyographic electricity from the masticatory muscles of mice in an awake state as claimed in claim 1, characterized in that: The end of the recording probe (400) is a cone, the number of the barbs (401) is at least two, and the barbs (401) are arranged on the circumference of the recording probe (400).

6. The electrode for collecting electromyographic electromyography of the masticatory muscles of mice in awake state as claimed in claim 1, characterized in that: The invention comprises a plurality of flexible electrode wires (300) and a plurality of recording probes (400), wherein at least some of the recording probes (400) and / or at least some of the flexible electrode wires (300) have different sizes, and is used for simultaneously detecting four masticatory muscles, namely, the masseter muscle, the temporalis muscle, the medial pterygoid muscle and the lateral pterygoid muscle.

7. A method for preparing an electrode for collecting electromyographic signals of masticatory muscles of mice in awake state as claimed in any one of claims 1 to 6, characterized in that: The following steps are involved: Manufacturing a flexible electrode line (300), a base (100), a recording probe (400), and a female connector (200); A barb (401) is provided at the tip of the recording probe (400), the tail of the recording probe (400) is fixedly connected to the end of the flexible electrode line (300), the other end of the flexible electrode line (300) is connected to the female connector (200), and the female connector is connected to the base (100).

8. The method for preparing the electrode for collecting electromyographic information of the masticatory muscles of mice in awake state as claimed in claim 7, characterized in that: The method for manufacturing a flexible electrode line (300) comprises: The conductive core layer (320) and the metal wire are arranged side by side at equal distances, and both ends of the metal wire are shorter than the conductive core layer (320); Wrapping the conductive core layer (320) and the metal wire with an insulating layer (310); The insulating skin layer (310) is drilled until the metal wire is exposed, the metal wire is drawn out of the hole, and an air extraction and release mechanism is connected to the hole to obtain a flexible electrode wire (300) with an airway (311).

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