A PCR instrument with a thermal cycle structure

Abstract

The present invention belongs to the technical field of PCR instruments, and in particular to a PCR instrument with a thermal cycle structure. The PCR instrument with a thermal cycle structure comprises a PCR instrument body mainly composed of a shell, an operation panel, three bases and three "U"-shaped mounting frames, the operation panel is installed on the front side of the shell, three material holes are sequentially opened on the top of the shell from left to right, and three bases are correspondingly arranged directly below the material holes, the bottom end of the base is fixedly connected to the shell, the mounting frame is installed on the top of each base, and a placement mechanism for placing a material to be tested is installed on the inner side of each mounting frame, and an adjustment mechanism for assisting in adjusting the placement mechanism is installed on each group of the placement mechanisms, so as to improve the accuracy and quality of uniform heating, thereby improving the detection effect of the device, and facilitating the device to detect sheet samples or solution samples according to actual detection needs, and convenient for switching.

Description

Technical Field

[0001] The invention belongs to the technical field of PCR instruments, and in particular relates to a PCR instrument with a thermal cycle structure. Background Art

[0002] Polymerase Chain Reaction (PCR), also known as cell-free molecular cloning or specific DNA sequence in vitro primer-directed enzymatic amplification technology, is a method of in vitro enzymatic synthesis of specific DNA fragments. It consists of a cycle of high-temperature denaturation, low-temperature annealing and temperature-appropriate extension, which is repeated to rapidly amplify the target DNA. PCR gene amplifier has the characteristics of strong specificity, high sensitivity, simple operation and time saving. It can not only be used for basic research such as gene isolation, cloning and nucleic acid sequence analysis, but also for disease diagnosis or any place with DNA and RNA. Therefore, it is widely used and rapidly developed in many fields such as molecular biology, microbiology, medicine and genetics.

[0003] When using a traditional PCR instrument, it is necessary to maintain thermal uniformity so that the sample is heated evenly. The current heating method is achieved through Peltier. The thermal uniformity of the current equipment is poor and it is not convenient to adjust according to the situation of the sample. The sample may be a solution or a sheet sample (a sample carried on a glass slide) that is difficult to adapt to the situation of the sample. The traditional PCR is also not convenient to adjust the heat supply of the Peltier operation according to actual needs, which leads to poor thermal uniformity.

[0004] According to the Chinese patent application number CN202121958274.3, in particular, it relates to a thermal cycle structure of a PCR amplification platform, including a reaction tube support, a thermal cycle block is arranged below the reaction tube support, the thermal cycle block includes a plurality of heating units arranged in an array, the heating unit includes a fixed block, a heat preservation body, an external fixed plate, a Peltier and a heating block; a first groove is arranged on both sides of the fixed block, the heat preservation body is arranged in the first groove, the heating block is arranged inside the heat preservation body, and a plurality of plug holes are arranged on the heating block; the Peltier is arranged near the heating block, and the external fixed plate is arranged outside the heat preservation body. The utility model can heat the reaction tube quickly and evenly, and can quickly cool down the heating unit; it also has temperature detection and thermal protection functions.

[0005] In combination with the above, the thermal uniformity is not stable enough and the operation of the Peltier cannot be adjusted according to the actual conditions of the sample.

[0006] In other words, the thermal uniformity of the PCR instrument is still not stable and reliable enough.

[0007] The existing technology has the following problems:

[0008] In order to solve the above problems, the present application proposes a PCR instrument with a thermal cycle structure. Summary of the invention

[0009] In order to solve the problems raised in the above background technology, the present invention provides a PCR instrument with a thermal cycle structure, which has the characteristics of uniform heat and convenient switching according to sample conditions.

[0010] To achieve the above-mentioned purpose, the present invention provides the following technical solutions: a PCR instrument with a thermal cycle structure, comprising a PCR instrument body mainly composed of a shell, an operation panel, three bases and three "U"-shaped mounting frames, the operation panel is installed on the front side of the shell, three material holes are sequentially opened on the top of the shell from left to right, and three bases are correspondingly arranged directly below the material holes, the bottom end of the base is fixedly connected to the shell, the mounting frame is installed on the top of each base, a placement mechanism for placing a material to be tested is installed on the inner side of each mounting frame, an adjustment mechanism for assisting in adjusting the placement mechanism is installed on each group of the placement mechanisms, and a thermal cycle mechanism for thermal cycling is installed on the inner side of each group of the placement mechanisms;

[0011] Each group of the placement mechanism includes a barrel, a spacer, a discharge assembly, and an observation assembly. The barrel is fixedly connected to the inner side of the mounting frame, the spacer is fixedly connected to the inner side of the bottom end of the barrel, the spacer passes through the bottom of the barrel, the spacer opens downward, the discharge assembly is slidably mounted on the barrel, and the observation assembly is detachably connected to the top of the discharge assembly;

[0012] Each group of the adjustment mechanism comprises a driving assembly, two limiting screws and two connecting frames, the driving assembly is installed inside the mounting frame, two limiting screws are slidably installed inside the driving assembly, the top end of the limiting screw is fixedly connected to the connecting frame, and one side of the connecting frame is connected to the discharge assembly;

[0013] Each group of the thermal circulation mechanism includes a heating component, a control component, an electric telescopic rod, and a top block. The heating component is fixedly installed on the inner side of the spacer, and the control component is installed on the heating component. The inner side of the corresponding base is fixedly connected to the electric telescopic rod, and the top block is fixedly connected to the output end of the electric telescopic rod.

[0014] As a preferred embodiment of a PCR instrument with a thermal cycle structure of the present invention, the adjustment mechanism further includes two limiting nuts, the outer side of the connecting frame is arranged in a threaded shape, and the outer side of the limiting nuts is threadedly connected to the connecting frame.

[0015] As a preferred PCR instrument with a thermal cycle structure of the present invention, each group of the discharge components is mainly composed of an adjusting sleeve and a fitting sleeve, the adjusting sleeve is slidably connected to the barrel, a sliding groove is provided on the inner side of the adjusting sleeve, the inner side wall of the barrel is slidably connected to the sliding groove, the outer side wall of the barrel is threadedly connected to the sliding groove, the fitting sleeve is slidably connected to the inner side wall of the barrel, the top end of the fitting sleeve is fixedly connected to the adjusting sleeve, a through hole is provided on the inner side of the fitting sleeve, the inner side wall of the through hole is slidably connected to the outer side of the spacer sleeve, and one side of the connecting frame is fixedly connected to the adjusting sleeve.

[0016] As a preferred PCR instrument with a thermal cycle structure of the present invention, each group of the observation components is mainly composed of a mounting sleeve, a lens and a gasket, the mounting sleeve is threadedly connected to the outer side of the top end of the adjustment sleeve, the lens is fixedly connected to the inner side of the top end of the mounting sleeve, the gasket is provided under the lens, and the outer side of the gasket is fixedly connected to the inner wall of the mounting sleeve.

[0017] As a preferred PCR instrument with a thermal cycle structure of the present invention, each group of the driving components is mainly composed of a motor, a driving wheel, a driven wheel, a turntable and a limiting ring. The motor is fixedly connected to the outside of the mounting frame, and the driving wheel is fixedly connected to the output end of the motor. A transmission hole is provided on the inside of the mounting frame at a position corresponding to the driving wheel, and the transmission hole penetrates the surface of the mounting frame. The driving wheel is arranged on the inside of the transmission hole, and the driven wheel is meshed with the right side of the driving wheel. The turntable is provided on the inside of the driven wheel, and two limiting grooves are symmetrically provided on the inside of the turntable. The inner side walls of the limiting grooves are slidably connected to the limiting screw. The limiting rings are provided at the top and bottom ends of the turntable, and the outer side of the limiting ring is fixedly connected to the inner side wall of the mounting frame. The top and bottom ends of the turntable are respectively slidably connected to the corresponding limiting rings.

[0018] As a preferred PCR instrument with a thermal cycle structure of the present invention, each group of the heating components is mainly composed of fifteen groups of Pani stickers A, sixty negative electrode trigger connectors A, Pani stickers B and negative electrode trigger connector B, the fifteen groups of Pani stickers A are fixedly connected to the inner side of the spacer, the number of Pani stickers A in each group is four, the sides of each group of four Pani stickers A that are close to each other are fixedly connected to the negative electrode trigger connector A, the Pani sticker B is fixedly connected to the inner side of the top of the spacer, and the negative electrode trigger connector B is fixedly connected to the bottom of the Pani sticker B.

[0019] As a preferred PCR instrument with a thermal cycle structure of the present invention, the control component is mainly composed of a power adapter, a positive wire, a negative wire and a trigger rod, the base is fixedly connected to the power adapter on one side, the positive terminal of the power adapter is fixedly connected to the positive wire, the positive wire runs through the surface of the barrel, one end of the positive wire is fixedly connected to the panitie A located on the left side of the bottom end, the negative terminal of the power adapter is fixedly connected to the negative wire, and the other end of the negative wire is fixedly connected to the trigger rod.

[0020] As a preferred PCR instrument with a thermal cycle structure of the present invention, the movement trajectory of the trigger rod is linear, the negative pole trigger connector A is arranged on the movement trajectory of the trigger rod, the outer side of the trigger rod is slidably connected to the negative pole trigger connector A, and the negative pole trigger connector B is arranged on the movement trajectory of the negative pole trigger connector A.

[0021] As a preferred PCR instrument with a thermal cycle structure of the present invention, an auxiliary heating mechanism for overheating protection is installed on one side of each group of the placement mechanism, and each group of the auxiliary heating mechanism includes a connecting tube A, a pump body, a solenoid valve, a connecting tube B and a heat exchange liquid tank, the right end of the barrel is fixedly connected to the connecting tube A, the right end of the connecting tube A is fixedly connected to the output end of the pump body, the input end of the pump body is fixedly connected to the connecting tube B, the solenoid valve is installed on the connecting tube B, the right end of the connecting tube B is fixedly connected to the heat exchange liquid tank, and the left side of the heat exchange liquid tank is fixedly connected to the mounting frame.

[0022] As a preferred PCR instrument with a thermal cycle structure of the present invention, the sixty Panitrap A are electrically connected to each other, the negative electrode line is electrically connected to the trigger rod, the Panitrap B is electrically connected to the Panitrap A located on the left side of the top, the control component is electrically connected to the operation panel, the electric telescopic rod is electrically connected to the operation panel, the motor is electrically connected to the operation panel, the pump body is electrically connected to the operation panel, and the solenoid valve is electrically connected to the operation panel.

[0023] Compared with the prior art, the present invention has the following beneficial effects:

[0024] 1. Through the placement mechanism, adjustment mechanism and thermal cycle mechanism, solution samples or sheet samples can be tested, the stability of the device is improved, the heating effect is improved, the accuracy and quality of uniform heating are improved, and the detection effect of the device is improved, which makes it convenient for the device to test sheet samples or solution samples according to actual detection needs and facilitate switching.

[0025] 2. Through the auxiliary heating mechanism, the heat exchange liquid fills the cavity below the fitting sleeve in the barrel, and the heat exchange liquid evenly heats the area around the glass slide, maintaining the temperature uniformity of the sample area as much as possible, reducing the temperature difference at the edge of the sample, and thus improving the detection effect of the device. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] The accompanying drawings are used to provide a further understanding of the present invention and constitute a part of the specification. Together with the embodiments of the present invention, they are used to explain the present invention and do not constitute a limitation of the present invention. In the accompanying drawings:

[0027] Figure 1 It is a schematic diagram of the overall structure of the present invention;

[0028] Figure 2 It is a schematic diagram of the installation structure of the installation frame in the present invention;

[0029] Figure 3 It is a schematic diagram of the installation structure of the placement mechanism in the present invention;

[0030] Figure 4 It is a schematic diagram of the installation structure of the discharge assembly in the present invention;

[0031] Figure 5 It is a schematic diagram of the installation structure of the observation assembly of the present invention;

[0032] Figure 6 It is a schematic diagram of the installation structure of the adjustment mechanism in the present invention;

[0033] Figure 7 It is a schematic diagram of the installation structure of the drive assembly in the present invention;

[0034] Figure 8 It is a schematic diagram of the installation structure of the thermal cycle mechanism in the present invention;

[0035] Fig. 9 It is a schematic diagram of the installation structure of the heating assembly of the present invention;

[0036] Fig.10 Schematic diagram of the installation structure of the negative electrode trigger connector A in the present invention;

[0037] Fig.11 It is a schematic diagram of the installation structure of the control component in the present invention;

[0038] Fig.12 It is a schematic diagram of the installation structure of the auxiliary heating mechanism in the present invention;

[0039] In the figure:

[0040] 1. PCR instrument body; 11. Shell; 12. Operation panel; 13. Base; 14. Mounting frame;

[0041] 2. Placement mechanism; 21. Cylinder; 22. Spacer; 23. Discharging assembly; 231. Adjustment sleeve; 232. Fitting sleeve; 24. Observation assembly; 241. Installation sleeve; 242. Lens; 243. Gasket;

[0042] 3. Adjustment mechanism; 31. Driving assembly; 311. Motor; 312. Driving wheel; 313. Driven wheel; 314. Turntable; 315. Limiting ring; 32. Limiting screw; 33. Connecting frame; 34. Limiting nut;

[0043] 4. Thermal cycle mechanism; 41. Heating assembly; 411. Panitrap A; 412. Negative trigger connector A; 413. Panitrap B; 414. Negative trigger connector B; 42. Control assembly; 421. Power adapter; 422. Positive wire; 423. Negative wire; 424. Trigger rod; 43. Electric telescopic rod; 44. Top block;

[0044] 5. Auxiliary heating mechanism; 51. Connecting pipe A; 52. Pump body; 53. Solenoid valve; 54. Connecting pipe B; 55. Heat exchange liquid tank. DETAILED DESCRIPTION

[0045] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0046] Example 1

[0047] like Figure 1-11 As shown;

[0048] A PCR instrument with a thermal cycle structure includes a PCR instrument body 1 mainly composed of a shell 11, an operation panel 12, three bases 13 and three "U"-shaped mounting frames 14. The operation panel 12 is installed on the front side of the shell 11. The top of the shell 11 is sequentially provided with three material holes from left to right. Three bases 13 are correspondingly arranged directly below the material holes. The bottom of the base 13 is fixedly connected to the shell 11. The top of each base 13 is installed with a mounting frame 14. The inner side of each mounting frame 14 is installed with a placement mechanism 2 for placing a material to be tested. Each group of placement mechanisms 2 is installed with an adjustment mechanism 3 for assisting in adjusting the placement mechanism 2, and a thermal cycle mechanism 4 for thermal cycle is installed inside each group of placement mechanisms 2; in an optional embodiment, each group of placement mechanisms 2 includes a barrel 21, a spacer 22, a discharge assembly 23, and an observation assembly 24, the inner side of the mounting frame 14 is fixedly connected with the barrel 21, the inner side of the bottom end of the barrel 21 is fixedly connected with the spacer 22, the spacer 22 passes through the bottom of the barrel 21, the spacer 22 opens downward, the discharge assembly 23 is slidably installed on the barrel 21, and the top of the discharge assembly 23 is detachably connected with the observation assembly 24;

[0049] Each group of adjustment mechanisms 3 includes a driving assembly 31, two limit screws 32 and two connecting frames 33. The driving assembly 31 is installed inside the mounting frame 14, and two limit screws 32 are slidably installed inside the driving assembly 31. The top of the limit screw 32 is fixedly connected to the connecting frame 33, and one side of the connecting frame 33 is connected to the discharge assembly 23.

[0050] Each set of thermal circulation mechanisms 4 includes a heating component 41, a control component 42, an electric telescopic rod 43, and a top block 44. The heating component 41 is fixedly installed on the inner side of the spacer 22, and the control component 42 is installed on the heating component 41. The inner side of the corresponding base 13 is fixedly connected to the electric telescopic rod 43, and the output end of the electric telescopic rod 43 is fixedly connected to the top block 44.

[0051] The adjustment mechanism 3 further includes two limiting nuts 34 . The outer side of the connecting frame 33 is arranged in a threaded shape, and the outer side of the limiting nuts 34 is threadedly connected to the connecting frame 33 .

[0052] In this embodiment: when the device is in use, first, the mounting sleeve 241 is rotated on the adjusting sleeve 231 to separate the mounting sleeve 241 from the adjusting sleeve 231, and then the sample is added to the barrel 21. Because the adjusting sleeve 231 is sleeved on the surface of the barrel 21, it can be understood that the sample is added to the adjusting sleeve 231, and then the mounting sleeve 241 is screwed on the outside of the adjusting sleeve 231, and the gasket 243 is tightly attached to the adjusting sleeve 231, so that the mounting sleeve 241 is reset, and the lens 242 is protected to avoid damage to the lens 242 due to a large twisting force. If the solution sample detected by the device is added at this time, the motor 311 drives the driving wheel 312 to rotate, and the driving wheel 312 is engaged with the driven wheel 313, so that the driving wheel 312 drives the driven wheel 313 to rotate. The driven wheel 313 rotates, and two upper and lower limit rings 315 limit the turntable 314, and then the driven wheel 313 drives the turntable 314 to rotate, and the limit screw 32 is placed in the limit groove of the turntable 314. The rotation of the turntable 314 drives the limit screw 32 to rotate, and the limit screw 32 drives the connecting frame 33 to rotate and then drives the adjusting sleeve 231 to rotate. The sliding groove of the adjusting sleeve 231 is threadedly connected with the outer wall of the barrel 21. The screw connection between the adjusting sleeve 231 and the barrel 21 allows the adjusting sleeve 231 to rise or fall relative to the barrel 21. At this time, the adjusting sleeve 231 is adjusted to fall so that the fitting sleeve 232 is close to the inner bottom of the barrel 21. The fitting sleeve 232 and the spacer sleeve 22 are slidably connected to each other, and the solution on the fitting sleeve 232 will not flow through the fitting sleeve 232, and then the electric telescopic rod 43 runs to drive the top block 44 to move left and right. The inclined setting of the top block 44 makes it possible for the top block 44 to move to the left and push the trigger rod 424 upward. The top block 44 moves to the right, and the trigger rod 424 falls downward under gravity and fits with the top block 44. At this time, the top block 44 limits the trigger rod 424, and then the position of the trigger rod 424 relative to the spacer sleeve 22 can be adjusted. It can be understood that the position of the trigger rod 424 in the spacer sleeve 22, that is, the trigger rod 424 contacts with several layers of negative trigger connectors A412, and the trigger rod 424 contacts with the negative trigger connectors A412 of the appropriate number of layers from bottom to top, and the positive pole of the power adapter 421 supplies power to the positive pole line 422, and the positive pole line 422 supplies power to the pani patch A411, The trigger rod 424 contacts the negative trigger connector A412, and the trigger rod 424 is connected to the negative pole of the power adapter 421 through the negative line 423, so that the power adapter 421, the positive line 422, the Panitrap A411, the negative trigger connector A412, the trigger rod 424, the negative line 423 and the power adapter 421 form a closed loop, so that the number of layers of the Panitrap A411 running is roughly equal to the height of the solution in the adjustment sleeve 231, reducing energy loss and avoiding uneven solution temperature caused by the operation of all the Panitrap A411. If the Panitrap A411 on the solution is also running, heat will be transferred through the contact medium and air, thereby improving the stability of the device, improving the heating effect, and improving the accuracy and quality of uniform heating.Thereby improving the detection effect of the device. If the device detects sheet samples, the top surface of the fitting sleeve 232 and the top surface of the spacer sleeve 22 are adjusted to be level with each other through the adjustment mechanism 3, and then the installation sleeve 241 is unscrewed, and the material is placed on the top surface of the spacer sleeve 22 and the top surface of the fitting sleeve 232. After resetting the installation sleeve 241, the electric telescopic rod 43 is extended, so that the top block 44 pushes the trigger rod 424 to contact the negative trigger connector B414, so that the power adapter 421, the positive line 422, the panitrap A411, the negative trigger connector A412, the trigger rod 424, and the negative line 423 form a closed loop with the power adapter 421, and the power adapter 421, the positive line 422, the panitrap B413, the negative trigger connector B414, the trigger rod 424, and the negative line 423 form a closed loop with the power adapter 421, so that the panitrap B413 is operated to heat the sheet sample. At this time When the fitting sleeve 232 rises, the solenoid valve 53 opens, and the pump body 52 runs to extract the heat exchange liquid through the heat exchange liquid tank 55, and injects it into the barrel 21 through the connecting pipe A51. The heat exchange liquid is located below the fitting sleeve 232, and then the operation of the paniter A411 heats the heat exchange liquid, and the heat exchange liquid fills the cavity below the fitting sleeve 232 in the barrel 21. The heat exchange liquid evenly heats the surrounding of the glass slide, and keeps the temperature uniformity of the sample area as much as possible, reduces the temperature difference at the edge of the sample, and thus improves the detection effect of the device, and facilitates the device to detect sheet samples or solution samples according to actual detection needs, and facilitates switching. By rotating the limit nut 34 on the limit screw 32, the limit nut 34 limits the movement range of the limit screw 32, and then the point of the maximum movement range of the limit screw 32 just makes the top surface of the fitting sleeve 232 and the top surface of the spacer sleeve 22 level with each other, and can also be adjusted arbitrarily. ,

[0053] like Figure 2 and Figure 6 As shown;

[0054] In an optional embodiment, the adjustment mechanism 3 further includes two limiting nuts 34 , the outer side of the connecting frame 33 is arranged in a threaded shape, and the outer side of the limiting nuts 34 is threadedly connected to the connecting frame 33 .

[0055] In this embodiment: In combination with the above, the limit nut 34 is rotated on the limit screw 32 so that the limit nut 34 limits the movement range of the limit screw 32, and then the point of the maximum movement range of the limit screw 32 just makes the top surface of the fitting sleeve 232 and the top surface of the spacer sleeve 22 level with each other, and can also be adjusted arbitrarily.

[0056] It should be noted that the back of the shell 11 should be provided with an operating hole for adjusting the limit nut 34. A back panel should be hinged in the operating hole. By opening the back panel, one can reach into the shell 11 to adjust the limit nut 34, which is also convenient for inspection and maintenance.

[0057] like Figure 2 , Figure 3 , Figure 4 and Figure 5 As shown;

[0058] In an optional embodiment, each group of discharge components 23 is mainly composed of an adjusting sleeve 231 and a fitting sleeve 232. The adjusting sleeve 231 is slidably connected to the barrel 21. A sliding groove is provided on the inner side of the adjusting sleeve 231. The inner wall of the barrel 21 is slidably connected to the sliding groove. The outer wall of the barrel 21 is threadedly connected to the sliding groove. The inner wall of the barrel 21 is slidably connected to the fitting sleeve 232. The top of the fitting sleeve 232 is fixedly connected to the adjusting sleeve 231. A through hole is provided on the inner side of the fitting sleeve 232. The inner wall of the through hole is slidably connected to the outer side of the spacer sleeve 22. One side of the connecting frame 33 is fixedly connected to the adjusting sleeve 231.

[0059] In this embodiment: In combination with the above, if the device detects a solution sample, the sample is added to the barrel 21. Because the adjusting sleeve 231 is sleeved on the surface of the barrel 21, it can be understood that the sample is added to the adjusting sleeve 231. The limiting screw 32 drives the connecting frame 33 to rotate and then drives the adjusting sleeve 231 to rotate. The slide groove of the adjusting sleeve 231 is threadedly connected to the outer wall of the barrel 21. The screw connection between the adjusting sleeve 231 and the barrel 21 allows the adjusting sleeve 231 to rise or fall relative to the barrel 21. At this time, the adjusting sleeve 231 is adjusted to fall so that the fitting sleeve 232 is close to the inner bottom of the barrel 21. The fitting sleeve 232 and the spacer sleeve 22 are slidably connected to each other, and the solution on the fitting sleeve 232 will not flow under the fitting sleeve 232. If the device detects a sheet sample, the top surface of the fitting sleeve 232 and the top surface of the spacer sleeve 22 are adjusted to be level with each other through the adjusting mechanism 3, and then the mounting sleeve 241 is unscrewed, and the material is placed on the top surface of the spacer sleeve 22 and the top surface of the fitting sleeve 232.

[0060] In an optional embodiment, each group of observation components 24 is mainly composed of a mounting sleeve 241, a lens 242 and a gasket 243. The mounting sleeve 241 is threadedly connected to the outer side of the top end of the adjustment sleeve 231, and the lens 242 is fixedly connected to the inner side of the top end of the mounting sleeve 241. A gasket 243 is provided under the lens 242, and the outer side of the gasket 243 is fixedly connected to the inner wall of the mounting sleeve 241.

[0061] In this embodiment: In combination with the above, firstly, the mounting sleeve 241 is rotated on the adjusting sleeve 231 to separate the mounting sleeve 241 from the adjusting sleeve 231, and then the sample is added to the barrel 21. Since the adjusting sleeve 231 is sleeved on the surface of the barrel 21, it can be understood that the sample is added to the adjusting sleeve 231. Then, the mounting sleeve 241 is screwed on the outside of the adjusting sleeve 231, and the gasket 243 is tightly attached to the adjusting sleeve 231, so that the mounting sleeve 241 is reset and the lens 242 is protected to avoid damage to the lens 242 due to a large twisting force.

[0062] like Figure 2 , Figure 6 and Figure 7 As shown;

[0063] In an optional embodiment, each driving assembly 31 is mainly composed of a motor 311, a driving wheel 312, a driven wheel 313, a turntable 314 and a limiting ring 315. The motor 311 is fixedly connected to the outer side of the mounting frame 14, and the driving wheel 312 is fixedly connected to the output end of the motor 311. A transmission hole is provided on the inner side of the mounting frame 14 at a position corresponding to the driving wheel 312, and the transmission hole passes through the surface of the mounting frame 14. The driving wheel 312 is arranged on the inner side of the transmission hole, and the driven wheel 313 is meshed with the right side of the driving wheel 312. A turntable 314 is provided on the inner side of the driven wheel 313, and two limiting grooves are symmetrically provided on the inner side of the turntable 314. The inner side walls of the limiting grooves are slidably connected to the limiting screw 32. Limiting rings 315 are provided at the top and bottom ends of the turntable 314. The outer side of the limiting ring 315 is fixedly connected to the inner side wall of the mounting frame 14, and the top and bottom ends of the turntable 314 are respectively slidably connected to the corresponding limiting rings 315.

[0064] In this embodiment: In combination with the above, if the device detects a solution sample, after the sample is added, the motor 311 runs to drive the driving wheel 312 to rotate, the driving wheel 312 engages with the driven wheel 313, and then the driving wheel 312 drives the driven wheel 313 to rotate, and the two upper and lower limit rings 315 limit the turntable 314, and then the driven wheel 313 drives the turntable 314 to rotate, the limit screw 32 is placed in the limit groove of the turntable 314, the rotation of the turntable 314 drives the limit screw 32 to rotate, the limit screw 32 drives the connecting frame 33 to rotate and then drives the adjusting sleeve 231 to rotate, the slide groove of the adjusting sleeve 231 is threadedly connected to the outer wall of the barrel 21, and the screw connection between the adjusting sleeve 231 and the barrel 21 allows the adjusting sleeve 231 to rise or fall relative to the barrel 21.

[0065] like Figure 2 , Figure 8 , Fig. 9 , Fig.10 and Fig.11 As shown;

[0066] In an optional embodiment, each group of heating components 41 is mainly composed of fifteen groups of pani stickers A411, sixty negative pole trigger connectors A412, pani stickers B413 and negative pole trigger connector B414, fifteen groups of pani stickers A411 are fixedly connected to the inner side of the spacer sleeve 22, the number of each group of pani stickers A411 is four, and the sides of each group of four pani stickers A411 that are close to each other are fixedly connected to the negative pole trigger connector A412, the inner side of the top end of the spacer sleeve 22 is fixedly connected to the pani sticker B413, and the bottom end of the pani sticker B413 is fixedly connected to the negative pole trigger connector B414.

[0067] In an optional embodiment, the control component 42 is mainly composed of a power adapter 421, a positive line 422, a negative line 423 and a trigger rod 424. The base 13 is fixedly connected to the power adapter 421 on one side, the positive terminal of the power adapter 421 is fixedly connected to the positive line 422, the positive line 422 runs through the surface of the barrel 21, one end of the positive line 422 is fixedly connected to the panitie A411 located on the left side of the bottom, the negative terminal of the power adapter 421 is fixedly connected to the negative line 423, and the other end of the negative line 423 is fixedly connected to the trigger rod 424.

[0068] In an optional embodiment, the movement trajectory of the trigger rod 424 is linear, the negative pole trigger connector A412 is arranged on the movement trajectory of the trigger rod 424, the outer side of the trigger rod 424 is slidingly connected to the negative pole trigger connector A412, and the negative pole trigger connector B414 is arranged on the movement trajectory of the negative pole trigger connector A412.

[0069] In this embodiment: In combination with the above, if the device detects a solution sample, the electric telescopic rod 43 drives the top block 44 to move left and right, and the top block 44 is set in an inclined shape, so that the top block 44 moves to the left to push the trigger rod 424 upward, and the top block 44 moves to the right, and the trigger rod 424 falls downward under gravity and fits with the top block 44. At this time, the top block 44 limits the trigger rod 424, and then the position of the trigger rod 424 relative to the spacer 22 can be adjusted. It can be understood that the position of the trigger rod 424 in the spacer 22, that is, the trigger rod 424 contacts the negative electrode trigger connector A412 of several layers, and the trigger rod 424 moves from bottom to top. The trigger rod 424 contacts the negative trigger connector A412 with a suitable number of layers, and the positive line 422 is powered by the positive electrode of the power adapter 421, and the positive line 422 powers the panitrap A411. The trigger rod 424 contacts the negative trigger connector A412, and the trigger rod 424 is connected to the negative electrode of the power adapter 421 through the negative line 423, so that the power adapter 421, the positive line 422, the panitrap A411, the negative trigger connector A412, the trigger rod 424, the negative line 423 and the power adapter 421 form a closed loop, so that the number of layers of the panitrap A411 is roughly the same as the number of layers of the solution in the regulating sleeve 23. 1 is equal in height, which reduces energy loss and avoids uneven solution temperature caused by the operation of all Panitrap A411. If the Panitrap A411 on the solution is also in operation, heat will be transferred through the contact medium and air, thereby improving the stability of the device, the heating effect, the accuracy and quality of uniform heating, and the detection effect of the device. If the device detects sheet samples, the top surface of the fitting sleeve 232 and the top surface of the spacer sleeve 22 are adjusted to be level with each other through the adjustment mechanism 3, and then the installation sleeve 241 is unscrewed, and the material is placed on the top surface of the spacer sleeve 22 and the top surface of the fitting sleeve 232, and the installation is reset. After the sleeve 241 is installed, the electric telescopic rod 43 is extended, so that the top block 44 pushes the trigger rod 424 to contact the negative trigger connector B414, so that the power adapter 421, the positive wire 422, the panitrap A411, the negative trigger connector A412, the trigger rod 424, the negative wire 423 and the power adapter 421 form a closed loop, and the power adapter 421, the positive wire 422, the panitrap B413, the negative trigger connector B414, the trigger rod 424, the negative wire 423 and the power adapter 421 form a closed loop, so that the panitrap B413 is operated to heat the sheet sample.

[0070] like Figure 2 and Fig.12 As shown;

[0071] In an optional embodiment, an auxiliary heating mechanism 5 for overheating protection is installed on one side of each group of placement mechanisms 2, and each group of auxiliary heating mechanisms 5 includes a connecting pipe A51, a pump body 52, a solenoid valve 53, a connecting pipe B54 and a heat exchange liquid tank 55. The right end of the barrel 21 is fixedly connected to the connecting pipe A51, the right end of the connecting pipe A51 is fixedly connected to the output end of the pump body 52, the input end of the pump body 52 is fixedly connected to the connecting pipe B54, the solenoid valve 53 is installed on the connecting pipe B54, the right end of the connecting pipe B54 is fixedly connected to the heat exchange liquid tank 55, and the left side of the heat exchange liquid tank 55 is fixedly connected to the mounting frame 14.

[0072] In this embodiment: In combination with the above, if the device detects a sheet sample, the top surface of the fitting sleeve 232 and the top surface of the spacer sleeve 22 are adjusted to be level with each other through the adjustment mechanism 3, and then the installation sleeve 241 is unscrewed, and the material is placed on the top surface of the spacer sleeve 22 and the top surface of the fitting sleeve 232. After resetting the installation sleeve 241, the electric telescopic rod 43 is extended, so that the top block 44 pushes the trigger rod 424 to contact the negative trigger connector B414, thereby making the power adapter 421, the positive line 422, the pani patch A411, the negative trigger connector A412, the trigger rod 424, and the negative line 423 form a closed loop with the power adapter 421 and the power adapter 421, the positive line 422, the pani patch B413, the negative trigger connector B414, the trigger rod 424, and the negative line 423 Then a closed loop is generated with the power adapter 421, so that the Panitrap B413 operates to heat the sheet sample. At this time, when the bonding sleeve 232 rises, the solenoid valve 53 opens, and the pump body 52 operates to extract the heat exchange liquid through the heat exchange liquid tank 55, and injects it into the barrel 21 through the connecting pipe A51. The heat exchange liquid is located below the bonding sleeve 232. Then the operation of the Panitrap A411 heats the heat exchange liquid, and the heat exchange liquid fills the cavity below the bonding sleeve 232 in the barrel 21. At this time, the solenoid valve 53 is closed, and the heat exchange liquid evenly heats the surrounding area of ​​the glass slide to maintain the temperature uniformity of the sample area as much as possible, reduce the temperature difference at the edge of the sample, and thereby improve the detection effect of the device. When the bonding sleeve 232 moves downward, the solenoid valve 53 opens, and the bonding sleeve 232 pushes the heat exchange liquid into the heat exchange liquid tank 55.

[0073] like Figure 1-12 As shown;

[0074] In an optional embodiment, sixty panitrap A411 are electrically connected to each other, the negative electrode line 423 is electrically connected to the trigger rod 424, the panitrap B413 is electrically connected to the panitrap A411 located on the top left side, the control component 42 is electrically connected to the operation panel 12, the electric telescopic rod 43 is electrically connected to the operation panel 12, the motor 311 is electrically connected to the operation panel 12, the pump body 52 is electrically connected to the operation panel 12, and the solenoid valve 53 is electrically connected to the operation panel 12.

[0075] In this embodiment: combined with the above, it is convenient to control the overall power supply start and stop through the operation panel 12.

[0076] The working principle and use process of the present invention are as follows: when the device is in use, firstly, the mounting sleeve 241 is rotated on the adjusting sleeve 231 to separate the mounting sleeve 241 from the adjusting sleeve 231, and then the sample is added to the barrel 21. Since the adjusting sleeve 231 is sleeved on the surface of the barrel 21, it can be understood that the sample is added to the adjusting sleeve 231. Then, the mounting sleeve 241 is screwed on the outside of the adjusting sleeve 231, and the gasket 243 is tightly attached to the adjusting sleeve 231, so that the mounting sleeve 241 is reset and the lens 242 is protected to avoid damage to the lens 242 due to a large twisting force. If the device detects a solution sample, after the sample is added, the motor 311 is driven to rotate the driving wheel 312, and the driving wheel 312 is meshed with the driven wheel 313, so that the driving wheel 312 is engaged ... The wheel 312 drives the driven wheel 313 to rotate, and two upper and lower limit rings 315 limit the turntable 314, and then the driven wheel 313 drives the turntable 314 to rotate, and the limit screw 32 is placed in the limit groove of the turntable 314. The rotation of the turntable 314 drives the limit screw 32 to rotate, and the limit screw 32 drives the connecting frame 33 to rotate and then drives the adjusting sleeve 231 to rotate. The sliding groove of the adjusting sleeve 231 is threadedly connected with the outer wall of the barrel 21. The screw connection between the adjusting sleeve 231 and the barrel 21 allows the adjusting sleeve 231 to rise or fall relative to the barrel 21. At this time, the adjusting sleeve 231 is adjusted to fall so that the fitting sleeve 232 is close to the inner bottom of the barrel 21. The fitting sleeve 232 and the spacer sleeve 22 are slidably connected to each other, and the solution on the fitting sleeve 232 will not flow through The electric telescopic rod 43 is operated to drive the top block 44 to move left and right. The inclined setting of the top block 44 allows the top block 44 to move to the left and push the trigger rod 424 upward. The top block 44 moves to the right, and the trigger rod 424 falls downward under gravity and fits with the top block 44. At this time, the top block 44 limits the trigger rod 424, and then the position of the trigger rod 424 relative to the spacer sleeve 22 can be adjusted. It can be understood that the position of the trigger rod 424 in the spacer sleeve 22, that is, the trigger rod 424 contacts with several layers of negative trigger connectors A412, and the trigger rod 424 contacts with the negative trigger connectors A412 of the appropriate number of layers from bottom to top, and the positive pole of the power adapter 421 supplies power to the positive pole line 422, and the positive pole line 422 supplies power to the paniter A411. The trigger rod 424 contacts the negative trigger connector A412, and the trigger rod 424 is connected to the negative pole of the power adapter 421 through the negative line 423, so that the power adapter 421, the positive line 422, the Panitrap A411, the negative trigger connector A412, the trigger rod 424, the negative line 423 and the power adapter 421 form a closed loop, so that the number of layers of the Panitrap A411 running is roughly equal to the height of the solution in the adjustment sleeve 231, reducing energy loss and avoiding uneven solution temperature caused by the operation of all the Panitrap A411. If the Panitrap A411 on the solution is also running, heat will be transferred through the contact medium and air, thereby improving the stability of the device, improving the heating effect, and improving the accuracy and quality of uniform heating.Thereby improving the detection effect of the device, if the device detects a sheet sample, at this time, the top surface of the fitting sleeve 232 and the top surface of the spacer sleeve 22 are adjusted to be level with each other through the adjustment mechanism 3, and then the installation sleeve 241 is screwed down, and the material is placed on the top surface of the spacer sleeve 22 and the top surface of the fitting sleeve 232. After resetting the installation sleeve 241, the electric telescopic rod 43 is extended, so that the top block 44 pushes the trigger rod 424 to contact the negative trigger connector B414, thereby making the power adapter 421, the positive line 422, Panitrap A411, negative trigger connector A412, trigger rod 424, negative line 423 and power adapter 421 generate a closed loop, and power adapter 421, positive line 422, Panitrap B413, negative trigger connector B414, trigger rod 424, negative line 423 and power adapter 421 generate a closed loop, so that Panitrap B413 operates to heat the sheet sample. At this time, when the fitting sleeve 232 rises, the solenoid valve 53 opens, and the pump body 52 operates. The heat exchange liquid is extracted through the heat exchange liquid tank 55 and injected into the barrel 21 through the connecting pipe A51. The heat exchange liquid is located below the fitting sleeve 232. Then, the operation of the paniter A411 heats the heat exchange liquid. The heat exchange liquid fills the cavity below the fitting sleeve 232 in the barrel 21. At this time, the solenoid valve 53 is closed, and the heat exchange liquid uniformly heats the area around the glass slide to maintain the uniformity of the temperature in the sample area as much as possible, reduce the temperature difference at the edge of the sample, thereby improving the detection effect of the device, and facilitating the device to detect sheet samples or solution samples according to actual detection needs, and convenient for switching. When the fitting sleeve 232 moves downward, the solenoid valve 53 is opened, and the fitting sleeve 232 presses the heat exchange liquid into the heat exchange liquid tank 55. By rotating the limit nut 34 on the limit screw 32, the limit nut 34 limits the movement range of the limit screw 32, and then the point of the maximum movement range of the limit screw 32 just makes the top surface of the fitting sleeve 232 and the top surface of the spacer sleeve 22 level with each other, which can also be adjusted arbitrarily. ,

[0077] Finally, it should be noted that the above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art can still modify the technical solutions described in the aforementioned embodiments or replace some of the technical features therein by equivalents. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. A PCR instrument with a thermal cycle structure, characterized in that: The invention comprises a PCR instrument body (1) which is mainly composed of a shell (11), an operation panel (12), three bases (13) and three "U"-shaped mounting frames (14); the operation panel (12) is mounted on the front side of the shell (11); three material holes are sequentially opened at the top of the shell (11) from left to right; three bases (13) are correspondingly arranged directly below the material holes; the bottom of the base (13) is fixedly connected to the shell (11); the mounting frame (14) is mounted on the top of each base (13); a placement mechanism (2) for placing a material to be tested is mounted on the inner side of each mounting frame (14); an adjustment mechanism (3) for assisting in adjusting the placement mechanism (2) is mounted on each group of the placement mechanisms (2); and a thermal cycle mechanism (4) for thermal cycle is mounted on the inner side of each group of the placement mechanisms (2); Each group of the placement mechanism (2) comprises a barrel (21), a spacer (22), a discharge assembly (23), and an observation assembly (24); the barrel (21) is fixedly connected to the inner side of the mounting frame (14); the inner side of the bottom end of the barrel (21) is fixedly connected to the spacer (22); the spacer (22) passes through the bottom of the barrel (21); the opening of the spacer (22) faces downward; the discharge assembly (23) is slidably mounted on the barrel (21); and the observation assembly (24) is detachably connected to the top end of the discharge assembly (23); Each group of the adjusting mechanism (3) comprises a driving assembly (31), two limiting screws (32) and two connecting frames (33); the driving assembly (31) is installed inside the mounting frame (14); the two limiting screws (32) are slidably installed inside the driving assembly (31); the top end of the limiting screw (32) is fixedly connected to the connecting frame (33); one side of the connecting frame (33) is connected to the discharge assembly (23); Each group of the thermal circulation mechanism (4) comprises a heating component (41), a control component (42), an electric telescopic rod (43), and a top block (44); the heating component (41) is fixedly installed on the inner side of the spacer (22); the control component (42) is installed on the heating component (41); the inner side of the corresponding base (13) is fixedly connected with the electric telescopic rod (43); and the output end of the electric telescopic rod (43) is fixedly connected with the top block (44); The adjustment mechanism (3) further comprises two limiting nuts (34), the outer side of the connecting frame (33) is arranged in a threaded shape, and the outer side of the limiting nuts (34) is threadedly connected to the connecting frame (33); Each group of the discharge assembly (23) is mainly composed of an adjustment sleeve (231) and a fitting sleeve (232), the adjustment sleeve (231) is slidably connected to the barrel (21), a slide groove is provided on the inner side of the adjustment sleeve (231), the inner side wall of the barrel (21) is slidably connected to the slide groove, the outer side wall of the barrel (21) is threadedly connected to the slide groove, the inner side wall of the barrel (21) is slidably connected to the fitting sleeve (232), the top end of the fitting sleeve (232) is fixedly connected to the adjustment sleeve (231), a through hole is provided on the inner side of the fitting sleeve (232), the inner side wall of the through hole is slidably connected to the outer side of the spacer sleeve (22), and one side of the connecting frame (33) is fixedly connected to the adjustment sleeve (231); The observation assembly (24) is mainly composed of a mounting sleeve (241), a lens (242) and a gasket (243); the outer side of the top end of the adjustment sleeve (231) is threadedly connected to the mounting sleeve (241); the inner side of the top end of the mounting sleeve (241) is fixedly connected to the lens (242); the gasket (243) is provided below the lens (242); the outer side of the gasket (243) is fixedly connected to the inner side wall of the mounting sleeve (241); The driving assembly (31) is mainly composed of a motor (311), a driving wheel (312), a driven wheel (313), a rotating disk (314) and a limiting ring (315); the motor (311) is fixedly connected to the outside of the mounting frame (14); the driving wheel (312) is fixedly connected to the output end of the motor (311); a transmission hole is provided on the inside of the mounting frame (14) at a position corresponding to the driving wheel (312); the transmission hole passes through the surface of the mounting frame (14); the driving wheel (312) is arranged on the inside of the transmission hole; The driven wheel (313) is meshed with the right side of the driving wheel (312), the inner side of the driven wheel (313) is provided with the rotating disk (314), the inner side of the rotating disk (314) is symmetrically provided with two limiting grooves, the inner side walls of the limiting grooves are slidably connected to the limiting screw (32), the top and bottom ends of the rotating disk (314) are provided with limiting rings (315), the outer side of the limiting rings (315) is fixedly connected to the inner side wall of the mounting frame (14), and the top and bottom ends of the rotating disk (314) are respectively slidably connected to the corresponding limiting rings (315).

2. The PCR instrument with a thermal cycle structure according to claim 1, characterized in that: The heating component (41) is mainly composed of fifteen groups of pani stickers A (411), sixty negative pole trigger connectors A (412), pani stickers B (413) and negative pole trigger connector B (414). The fifteen groups of pani stickers A (411) are fixedly connected to the inner side of the spacer (22). The number of the pani stickers A (411) is four, and the sides of each group of four pani stickers A (411) close to each other are fixedly connected to the negative pole trigger connector A (412). The inner side of the top of the spacer (22) is fixedly connected to the pani sticker B (413), and the bottom of the pani sticker B (413) is fixedly connected to the negative pole trigger connector B (414).

3. The PCR instrument with a thermal cycle structure according to claim 2, characterized in that: The control component (42) mainly consists of a power adapter (421), a positive line (422), a negative line (423) and a trigger rod (424); the base (13) is fixedly connected to the power adapter (421) on one side; the positive terminal of the power adapter (421) is fixedly connected to the positive line (422); the positive line (422) passes through the surface of the barrel (21); one end of the positive line (422) is fixedly connected to the panitie A (411) located on the left side of the bottom; the negative terminal of the power adapter (421) is fixedly connected to the negative line (423); and the other end of the negative line (423) is fixedly connected to the trigger rod (424).

4. The PCR instrument with a thermal cycle structure according to claim 3, characterized in that: The movement trajectory of the trigger rod (424) is linear, the negative pole trigger connector A (412) is arranged on the movement trajectory of the trigger rod (424), the outer side of the trigger rod (424) is slidably connected to the negative pole trigger connector A (412), and the negative pole trigger connector B (414) is arranged on the movement trajectory of the negative pole trigger connector A (412).

5. The PCR instrument with a thermal cycle structure according to claim 4, characterized in that: An auxiliary heating mechanism (5) for overheat protection is installed on one side of the placement mechanism (2), and the auxiliary heating mechanism (5) includes a connecting pipe A (51), a pump body (52), a solenoid valve (53), a connecting pipe B (54) and a heat exchange liquid tank (55). The right end of the barrel (21) is fixedly connected to the connecting pipe A (51), the right end of the connecting pipe A (51) is fixedly connected to the output end of the pump body (52), the input end of the pump body (52) is fixedly connected to the connecting pipe B (54), the solenoid valve (53) is installed on the connecting pipe B (54), the right end of the connecting pipe B (54) is fixedly connected to the heat exchange liquid tank (55), and the left side of the heat exchange liquid tank (55) is fixedly connected to the mounting frame (14).

6. The PCR instrument with a thermal cycle structure according to claim 5, characterized in that: The sixty panitrap A (411) are electrically connected to each other, the negative electrode line (423) is electrically connected to the trigger rod (424), the panitrap B (413) is electrically connected to the panitrap A (411) located on the left side of the top, the control component (42) is electrically connected to the operation panel (12), the electric telescopic rod (43) is electrically connected to the operation panel (12), the motor (311) is electrically connected to the operation panel (12), the pump body (52) is electrically connected to the operation panel (12), and the solenoid valve (53) is electrically connected to the operation panel (12).

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