Preparation method of bird's nest peptide and application thereof

Abstract

The application discloses a preparation method of high-antioxidant-activity bird's nest peptides and application of the bird's nest peptides, and the method comprises the following steps: adding bird's nest fragments into a mixed solution of SDS and urea with a certain concentration, and dissolving all the bird's nest proteins, so that the fine feather and other impurities are separated from the bird's nest proteins, the manual picking of the bird's nest fragments is avoided, and 100% of the bird's nest fragments are utilized, the yield of the prepared bird's nest peptides is high, the bird's nest peptides have high antioxidant activity, and the bird's nest peptides have wide application prospects when applied to the preparation of whitening creams.

Description

Technical Field

[0001] This invention belongs to the field of bird's nest processing technology, specifically relating to a bird's nest peptide with high antioxidant activity and its preparation method. Background Technology

[0002] Edible bird's nest is the nest formed by the saliva and down feathers of swiftlets (Apodidae family), and it is believed to have various health benefits, including beauty enhancement and anti-aging. Currently, the main swiftlets producing edible bird's nest are the Greater Swiftlet and the Javanese Swiftlet. Global bird's nest production is concentrated in Southeast Asia, such as Indonesia, Thailand, and Malaysia, with Indonesia accounting for over 80% of global production. Bird's nest fragments are the fine materials generated during the harvesting, transportation, and processing of bird's nests. In traditional bird's nest processing, these fragments account for approximately 10% of the total raw material. Bird's nest fragments come in various shapes, including short strips, chunks, debris, or flakes, with a pale yellow surface. They contain grayish-black fine feather impurities that are difficult to separate, hindering commercial application. However, the basic components and bioactivity of bird's nest fragments are not significantly different from whole bird's nests; therefore, using bird's nest fragments as raw material to prepare highly antioxidant-active bird's nest peptides has broad application prospects.

[0003] Patent CN111406826A discloses a production method and filtration structure for bird's nest peptide products, including steps such as raw material collection, denaturation, enzymatic hydrolysis, primary heat treatment, secondary enzymatic hydrolysis, tertiary enzymatic hydrolysis, secondary inactivation, filtration to remove impurities, concentration, sterilization, and drying. Although this invention can improve the edibility of bird's nest, the prepared bird's nest peptides require multiple enzymatic hydrolysis processes, and their large molecular weight is not conducive to human digestion, absorption, and utilization, as well as the absorption of their active ingredients.

[0004] Therefore, how to provide a simple, easy-to-remove, and highly antioxidant bird's nest peptide preparation method using bird's nest fragments as raw material to achieve high added value utilization of bird's nest fragments is a technical problem that urgently needs to be solved by those skilled in the art. Summary of the Invention

[0005] The purpose of this section is to outline some aspects of embodiments of the present invention and to briefly describe some preferred embodiments. Simplifications or omissions may be made in this section, as well as in the abstract and title of this application, to avoid obscuring the purpose of these documents; however, such simplifications or omissions should not be construed as limiting the scope of the invention.

[0006] In view of the problems existing in the above and / or prior art, the present invention is proposed.

[0007] Therefore, the purpose of this invention is to overcome the shortcomings of the prior art and provide a method for preparing bird's nest peptides with high antioxidant activity.

[0008] To solve the above-mentioned technical problems, the present invention provides the following technical solution: including,

[0009] The bird's nest fragments are crushed, sieved, and soaked in a mixed solution of SDS and urea. After soaking, they are simmered until all the bird's nest protein is dissolved.

[0010] After dissolution, the first impurity removal and separation process is performed, and the permeate is collected.

[0011] The permeate is used for a second impurity removal and separation, and the solution of dissolved protein from broken swallow pieces is collected.

[0012] Flavor protease and papain were used to perform targeted enzymatic hydrolysis of the broken bird's nest protein, respectively.

[0013] After enzymatic hydrolysis, the enzyme is inactivated, and the target molecular weight bird's nest peptides are collected, concentrated, and dried to obtain the final product, bird's nest peptides.

[0014] As a preferred embodiment of the preparation method of the highly antioxidant active bird's nest peptide of the present invention, the mixed solution of SDS and urea has the following characteristics: the concentration of SDS is 0.8-2 mol / L, the concentration of urea is 0.8-2 mol / L, and the soaking time is 30-60 min.

[0015] In a preferred embodiment of the preparation method of the highly antioxidant active bird's nest peptide of the present invention, the stewing temperature is 80-100℃ and the time is 60-300 min.

[0016] As a preferred embodiment of the preparation method of the high antioxidant activity bird's nest peptide of the present invention, the first impurity removal separation includes filtering the stewed mixture through a filter cloth or an ultrafiltration membrane to remove impurities, wherein the mesh number of the filter cloth is not less than 200 mesh or the pore size of the ultrafiltration membrane is not greater than 1 μm.

[0017] As a preferred embodiment of the preparation method of the high antioxidant activity bird's nest peptide of the present invention, the following is provided: the amount of flavor protease added is 1000-2000U per gram of bird's nest fragments dissolved protein, and the enzymatic hydrolysis time is 20-40min; the amount of papain added is 800-1200U per gram of bird's nest fragments dissolved protein, and the enzymatic hydrolysis time is 30-60min.

[0018] In a preferred embodiment of the method for preparing the high antioxidant activity bird's nest peptides of the present invention, the bird's nest peptides with the target molecular weight are collected by ultrafiltration separation, wherein the molecular weight cutoff of ultrafiltration is 5000 Da.

[0019] As a preferred embodiment of the method for preparing the highly antioxidant bird's nest peptides of the present invention, the method yields bird's nest peptides with a molecular weight of less than 5000 Da, comprising up to 90% of the peptides, and exhibiting a free radical scavenging capacity of up to 450 μmol VCE g. -1 .

[0020] Another objective of this invention is to overcome the shortcomings of the prior art and provide an application of a bird's nest peptide with high antioxidant activity.

[0021] To solve the above-mentioned technical problems, the present invention provides the following technical solution: The high antioxidant activity bird's nest peptide is applied to the preparation of a whitening cream, wherein the cream comprises an oil phase and an aqueous phase, and the high antioxidant activity bird's nest peptide is added to the aqueous phase.

[0022] As a preferred embodiment of the preparation method of the high antioxidant active bird's nest peptide of the present invention, the oil phase of the cream comprises 1.5% octadecanol, 2% white oil, 1% GTCC, 1% jojoba oil, 1.5% monoglyceride, 1.5% phenobarbital-20, and 0.15% methylparaben.

[0023] The aqueous phase comprises 30% Carbomer 941, 2% glycerol, 1.5% butylene glycol, 5% highly antioxidant bird's nest peptide, and the remainder is water;

[0024] The cream is obtained by mixing the oil phase and the water phase.

[0025] Beneficial effects of this invention:

[0026] (1) This invention provides a method for preparing bird's nest peptides with high antioxidant activity. In view of the problem that bird's nest fragments contain a lot of fine feather impurities that are difficult to separate, this method can completely dissolve bird's nest protein, so that fine feathers and other impurities can be better separated from bird's nest protein. This avoids the need for manual picking of bird's nest fragments and achieves 100% utilization of bird's nest fragments.

[0027] (2) This invention creatively adds bird's nest fragments to a mixed solution of SDS and urea of ​​a certain concentration, which can synergistically and effectively destroy the hydrophobic interactions and hydrogen bonds between bird's nest proteins, so that the bird's nest proteins can be 100% dissolved during stewing. After two impurity removal separations, the first impurity removal separation is achieved by filtering with a filter cloth to remove 100% of impurities such as feathers. After the second impurity removal separation, the content of SDS, urea and nitrate in the bird's nest protein solution is 0, and the yield of bird's nest protein is 96%.

[0028] (3) In this invention, flavor protease and papain were used to enzymatically hydrolyze bird's nest protein under optimal conditions. Under these conditions, the yield of small molecule peptides was significantly improved and the antioxidant activity of bird's nest peptides was enhanced. The content of bird's nest peptides with a molecular weight of less than 5000 Da reached 90%, and the tyrosinase inhibition rate reached 80%, which has broad application prospects.

[0029] (4) The bird's nest peptide of the present invention was used to prepare a cream. Experiments showed that after applying the cream for 30 days, the skin melanin content (MI) value was significantly reduced and the skin brightness was significantly increased. This indicates that the bird's nest peptide prepared by the method of the present invention can exert a significant antioxidant function with a small amount of addition. Detailed Implementation

[0030] To make the above-mentioned objects, features and advantages of the present invention more apparent and understandable, the specific embodiments of the present invention will be described in detail below with reference to the examples in the specification.

[0031] Many specific details are set forth in the following description in order to provide a full understanding of the invention. However, the invention may also be practiced in other ways different from those described herein, and those skilled in the art can make similar extensions without departing from the spirit of the invention. Therefore, the invention is not limited to the specific embodiments disclosed below.

[0032] Secondly, the term "one embodiment" or "embodiment" as used herein refers to a specific feature, structure, or characteristic that may be included in at least one implementation of the present invention. The phrase "in one embodiment" appearing in different places in this specification does not necessarily refer to the same embodiment, nor is it a single or selective embodiment that is mutually exclusive with other embodiments.

[0033] The method for calculating protein yield in this embodiment of the invention is as follows:

[0034] Protein yield = Amount of protein collected / Amount of protein added * 100%;

[0035] The method for determining the molecular weight of bird's nest peptides refers to the People's Republic of China National Standard GB31645-2018.

[0036] The determination of the antioxidant properties of bird's nest peptides was based on the master's thesis by Wang Xin: Preparation and Research of Bird's Nest Peptide with High Whitening Activity [D], Wuxi: Jiangnan University, 2021.

[0037] Unless otherwise specified, all raw materials used in this invention are commercially available.

[0038] Example 1

[0039] Use a shredder to crush the swallow fragments containing fine feather impurities through a 60-mesh sieve, and set the sieve material aside.

[0040] Weigh 100g of the sieved material and add it to a mixed solution of 10L of 1mol / L urea and 1mol / L SDS. Soak at room temperature for 40 minutes and then stew the bird's nest at 100℃ for 120 minutes.

[0041] Use a 200-mesh filter cloth to filter the stewed bird's nest, discard the impurities obtained from the filter, and retain the filtrate;

[0042] The filtrate was filtered using an ultrafiltration system with a molecular weight cutoff of 1000 Da, and the retentate was collected to obtain the bird's nest protein solution.

[0043] First, flavor protease was used for enzymatic hydrolysis under optimal conditions. The amount of enzyme added was 1500U of protein dissolved per gram of broken bird's nest, and the hydrolysis time was 30 minutes.

[0044] Then, the papain was used for enzymatic hydrolysis under optimal conditions. The amount of enzyme added was 1000U per gram of broken bird's nest protein, and the hydrolysis time was 45 minutes.

[0045] After enzymatic hydrolysis, the enzyme was inactivated by boiling water at 100℃ for 15 minutes. The filtrate was then filtered using an ultrafiltration membrane with a molecular weight cutoff of 5000 Da, and the permeate was collected.

[0046] The collected bird's nest peptides were concentrated under normal pressure and freeze-dried to obtain bird's nest peptides.

[0047] Example 2

[0048] In this embodiment, the urea soaking process and protein extraction process were adjusted under the process conditions of Example 1, while other conditions were the same as those in Example 1. The conditions and results are shown in Table 1.

[0049] Table 1 Adjustments to Urea Soaking and Protein Extraction Processes

[0050]

[0051]

[0052] As can be seen from Table 1, when the concentrations of SDS and urea are low, the hydrogen bonds and hydrophobic bonds inside the bird's nest protein cannot be completely destroyed, and the bird's nest is not completely hydrated and dissolved. After the first filtration to remove impurities, the protein yield is low, only 75%. When the extraction temperature is too low, the bird's nest also cannot be completely hydrated and dissolved. After the first filtration to remove impurities, the protein yield is low, only 65%.

[0053] Furthermore, while increasing the stewing temperature can completely dissolve the bird's nest protein, the resulting small molecule bird's nest peptides have a lower tyrosinase inhibition rate of only 73%, which is significantly lower than the 80% in Example 1. When the stewing temperature is too high, the degree of Maillard reaction of bird's nest protein is increased, resulting in lower antioxidant activity of the resulting small molecule bird's nest peptides, which is only 44%.

[0054] Example 3

[0055] In this embodiment, under the process conditions of Example 1, the two impurity removal processes were adjusted to explore the effects of the two impurity removal separation conditions on the impurity removal effect and protein yield. The specific conditions and results are shown in Table 2.

[0056] Table 2 Adjustment of the two impurity removal and separation conditions

[0057]

[0058]

[0059] As can be seen from Table 2, when the mesh size of the filter cloth selected for the first impurity removal is small, it cannot effectively remove impurities such as feathers. When the mesh size is too large, although it can effectively remove feathers, it will also remove some protein, thereby reducing the yield of bird's nest protein.

[0060] If the molecular weight cutoff of the ultrafiltration used in the second impurity removal separation is too high, the yield of bird's nest protein will be reduced to only 92%. If the molecular weight cutoff is too low, urea cannot be completely removed under the same ultrafiltration conditions, and the ultrafiltration time needs to be increased. Therefore, only by selecting the optimal impurity removal separation conditions can the complete removal of urea be achieved while improving the yield of bird's nest protein.

[0061] Example 4

[0062] In this embodiment, under the process conditions of Example 1, the enzymatic hydrolysis process was adjusted to investigate the effect of enzymatic hydrolysis conditions on the molecular weight and free radical scavenging ability of the obtained bird's nest peptides. The results are shown in Table 3.

[0063] Table 3. Regulation of Enzymatic Hydrolysis Process

[0064]

[0065]

[0066] As can be seen from Table 3, even when the enzyme activity is doubled or the hydrolysis time is doubled, the content of bird's nest peptides with a molecular weight of less than 5000 Da and the antioxidant activity (free radical scavenging ability) are both low when flavor protease and papain are used alone. This indicates that the present invention achieves better quality bird's nest by hydrolyzing the product with two enzymes in sequence, thus achieving a synergistic effect.

[0067] Example 5

[0068] The whitening active small molecule bird's nest peptide obtained in Example 1 was made into a cream. The basic formula of the cream is shown in Table 4.

[0069] Table 4. Bird's Nest Peptide Cream Formula

[0070]

[0071]

[0072] The oil and water phases in Table 1 were mixed to prepare bird's nest peptide cream.

[0073] Ten volunteers aged 20-25 with no history of skin allergies were selected. Each volunteer was instructed to apply the cream twice daily, with an interval of more than 6 hours between applications. The application area was a circle approximately 2 cm in diameter on the inner side of the left forearm, with one circle containing a blank cream and the other containing a sample cream (choosing the inner left forearm helps prevent the influence of sun exposure on the results). Skin brightness (L-value) and melanin content (MI-value) were measured after 30 days. The results are shown in Table 5.

[0074] Table 5. Results of skin brightening and melanin measurement after 30 days of cream application.

[0075]

[0076] Table 5 shows that after 30 days, the L and MI values ​​of the skin treated with the blank cream remained basically unchanged. However, after 30 days, the skin brightness L value of the 10 volunteers who applied the cream containing bird's nest peptides increased significantly, while the skin melanin content MI value decreased significantly. This indicates that the bird's nest peptide cream prepared in this invention has a good whitening effect, and at the same time, it can exert a significant antioxidant function with a small amount of addition.

[0077] Comparative Example 1

[0078] This embodiment, under the process conditions of Example 1, regulates the enzymatic hydrolysis sequence to investigate the effect of the enzymatic hydrolysis sequence of flavor protease and papain on the molecular weight and free radical scavenging ability of the obtained bird's nest peptide.

[0079] Use a shredder to crush the swallow fragments containing fine feather impurities through a 60-mesh sieve, and set the sieve material aside.

[0080] Weigh 100g of the sieved material and add it to 10L of 3mol / L urea solution. Soak at room temperature for 60 minutes, then stew the bird's nest at 100℃ for 90 minutes.

[0081] Use a 200-mesh filter cloth to filter the stewed bird's nest, discard the impurities obtained from the filter, and retain the filtrate;

[0082] The filtrate was filtered using an ultrafiltration system with a molecular weight cutoff of 1000 Da, and the retentate was collected to obtain the bird's nest protein solution.

[0083] First, papain was used for enzymatic hydrolysis under optimal conditions. The amount of enzyme added was 1000U per gram of broken bird's nest protein, and the hydrolysis time was 45 minutes.

[0084] Then, the flavor protease was enzymatically hydrolyzed under optimal conditions, with an enzyme dosage of 1500U and a hydrolysis time of 30min.

[0085] After enzymatic hydrolysis, the enzyme was inactivated by boiling water at 100℃ for 15 minutes. The filtrate was then filtered using an ultrafiltration membrane with a molecular weight cutoff of 5000 Da, and the permeate was collected.

[0086] The collected bird's nest peptides were concentrated under normal pressure and freeze-dried to obtain bird's nest peptides.

[0087] At this point, the content of bird's nest peptides with a molecular weight less than 5000 Da was 85%, and the antioxidant activity (free radical scavenging ability) was 421 μmol VCE g. -1 Therefore, the order of enzymatic hydrolysis is crucial.

[0088] Comparative Example 2

[0089] Use a shredder to crush the swallow fragments containing fine feather impurities through a 60-mesh sieve, and set the sieve material aside.

[0090] Weigh 100g of the sieved material and add it to a mixed solution of 10L, 1mol / L urea and 1% SDS. Soak at room temperature for 60 minutes and then stew the bird's nest at 100℃ for 90 minutes.

[0091] Use a 200-mesh filter cloth to filter the stewed bird's nest, discard the impurities obtained from the filter, and retain the filtrate;

[0092] The filtrate was filtered using an ultrafiltration system with a molecular weight cutoff of 1000 Da, and the retentate was collected to obtain the bird's nest protein solution.

[0093] First, use pepsin to perform enzymatic hydrolysis under optimal conditions. The amount of enzyme added is 1500U of protein dissolved per gram of broken swallow pieces, and the hydrolysis time is 30 minutes.

[0094] Then, enzymatic hydrolysis was carried out with trypsin under optimal conditions, with an enzyme dosage of 1000U per gram of broken swallow protein and a hydrolysis time of 45 minutes.

[0095] After enzymatic hydrolysis, the enzyme was inactivated by boiling water at 100℃ for 15 minutes. The filtrate was then filtered using an ultrafiltration membrane with a molecular weight cutoff of 5000 Da, and the permeate was collected.

[0096] The collected bird's nest peptides were concentrated under normal pressure and freeze-dried to obtain bird's nest peptides.

[0097] Comparative Example 3

[0098] Use a shredder to crush the swallow fragments containing fine feather impurities through a 60-mesh sieve, and set the sieve material aside.

[0099] Weigh 100g of the sieved material and add it to 10L of 3mol / L urea solution. Soak at room temperature for 60 minutes, then stew the bird's nest at 100℃ for 90 minutes.

[0100] Use a 200-mesh filter cloth to filter the stewed bird's nest, discard the impurities obtained from the filter, and retain the filtrate;

[0101] The filtrate was filtered using an ultrafiltration system with a molecular weight cutoff of 1000 Da, and the retentate was collected to obtain the bird's nest protein solution.

[0102] First, flavor protease was used for enzymatic hydrolysis under optimal conditions. The amount of enzyme added was 1500U of protein dissolved per gram of broken bird's nest, and the hydrolysis time was 30 minutes.

[0103] The papain was used for enzymatic hydrolysis under optimal conditions, with an enzyme dosage of 1000U per gram of broken egg white protein and a hydrolysis time of 45 minutes.

[0104] After enzymatic hydrolysis, the enzyme was inactivated by boiling water at 100℃ for 15 minutes. The filtrate was then filtered using an ultrafiltration membrane with a molecular weight cutoff of 5000 Da, and the permeate was collected.

[0105] The collected bird's nest peptides were concentrated under normal pressure and freeze-dried to obtain bird's nest peptides.

[0106] Comparative Example 4

[0107] Use a shredder to crush the swallow fragments containing fine feather impurities through a 60-mesh sieve, and set the sieve material aside.

[0108] Weigh 100g of the sieved material and add it to 10L of 3mol / L SDS solution. Soak at room temperature for 60 minutes, then stew the bird's nest at 100℃ for 90 minutes.

[0109] Use a 200-mesh filter cloth to filter the stewed bird's nest, discard the impurities obtained from the filter, and retain the filtrate;

[0110] The filtrate was filtered using an ultrafiltration system with a molecular weight cutoff of 1000 Da, and the retentate was collected to obtain the bird's nest protein solution.

[0111] First, flavor protease was used for enzymatic hydrolysis under optimal conditions. The amount of enzyme added was 1500U of protein dissolved per gram of broken bird's nest, and the hydrolysis time was 30 minutes.

[0112] The papain was used for enzymatic hydrolysis under optimal conditions, with an enzyme dosage of 1000U per gram of broken egg white protein and a hydrolysis time of 45 minutes.

[0113] After enzymatic hydrolysis, the enzyme was inactivated by boiling water at 100℃ for 15 minutes. The filtrate was then filtered using an ultrafiltration membrane with a molecular weight cutoff of 5000 Da, and the permeate was collected.

[0114] The collected bird's nest peptides were concentrated under normal pressure and freeze-dried to obtain bird's nest peptides.

[0115] The molecular weight and free radical scavenging ability of the bird's nest peptides prepared in Comparative Examples 1 to 4 were determined and compared with those in Example 1. The results are shown in Table 6.

[0116] Table 6. Molecular weight and free radical scavenging ability of bird's nest peptides in the examples and comparative examples.

[0117]

[0118] As can be seen from Table 6, by soaking the bird's nest raw material in a mixed solution of SDS and urea, the present invention can synergistically destroy the hydrophobic interactions and hydrogen bonds between bird's nest proteins, so that the bird's nest protein can be 100% dissolved during stewing. After filtration with a filter cloth, feather lamp impurities are 100% removed. After the second impurity removal and separation, the content of SDS, urea and nitrate in the bird's nest protein solution is 0, and the yield of bird's nest protein is 96%.

[0119] Meanwhile, this invention uses flavor protease and neutral protease to enzymatically hydrolyze bird's nest protein, which significantly improves the yield of small molecule peptides and the antioxidant activity of bird's nest peptides under optimal conditions, and has broad application prospects.

[0120] It should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and are not intended to limit it. Although the present invention has been described in detail with reference to preferred embodiments, those skilled in the art should understand that modifications or equivalent substitutions can be made to the technical solutions of the present invention without departing from the spirit and scope of the technical solutions of the present invention, and all such modifications or substitutions should be covered within the scope of the claims of the present invention.

Claims

1. A method for preparing a bird's nest peptide, characterized by: comprising, The bird's nest raw material is crushed and sieved, and then added into a mixed solution of SDS and urea for soaking. After soaking, the bird's nest protein is completely dissolved by stewing; The mixed solution of SDS and urea, wherein the concentration of SDS is 0.8-2 mol / L, the concentration of urea is 0.8-2 mol / L, and the soaking time is 30-60 min; After dissolution, the first impurity removal and separation are performed, and the permeate is collected; The permeate is subjected to the second impurity removal and separation, and the bird's nest dissolved protein solution is collected; The bird's nest dissolved protein is subjected to directional enzymolysis using flavor protease and papain in sequence; The enzyme amount of the flavor protease is 1000-2000 U per gram of bird's nest dissolved protein, and the enzymeolysis time is 20-40 min; the enzyme amount of the papain is 800-1200 U per gram of bird's nest dissolved protein, and the enzymeolysis time is 30-60 min. After enzyme inactivation, the bird's nest peptide with a target molecular weight is collected, concentrated and dried to obtain the final product of the bird's nest peptide.

2. The method of claim 1, wherein the method is characterized by: The stewing temperature is 80-100℃, and the time is 60-300 min.

3. The method of claim 1, wherein the method is characterized by: The first impurity removal and separation include filtering the mixed solution after stewing through filter cloth or ultrafiltration membrane, wherein the mesh number of the filter cloth is not less than 200 meshes or the pore size of the ultrafiltration is not greater than 1 μm.

4. The method of claim 1, wherein the method is characterized by: The second impurity removal and separation include ultrafiltration impurity removal and separation, wherein the molecular weight cut-off of the ultrafiltration is 1000 Da.

5. The method of claim 1, wherein the method is characterized by: The collection of the bird's nest peptide with a target molecular weight is performed by ultrafiltration separation, wherein the molecular weight cut-off of the ultrafiltration is 5000 Da.

6. The bird's nest peptide prepared by the method of any one of claims 1-5.

7. The use of the bird's nest peptide according to claim 6, wherein the bird's nest peptide is administered in an amount of 0.1 to 100 mg per day. The prepared bird's nest peptide is applied to the preparation of a cream with whitening function, wherein the cream includes an oil phase and an aqueous phase, and the active bird's nest peptide is added to the aqueous phase.

8. The use of the bird's nest peptide according to claim 7, wherein the bird's nest peptide is administered in an amount of 0.1-100 mg / kg. The preparation method of the cream includes, In terms of the mass percentage of the cream, the oil phase includes 1.5% octadecanol, 2% white oil, 1% GTCC, 1% jojoba oil, 1.5% monoglyceride, 1.5% peregal-20, and 0.15% ni platinum gold methyl ester; The aqueous phase includes 30% carbowax 941, 2% glycerol, 1.5% butanediol, 5% bird's nest peptide, and the balance is water; The oil phase and the aqueous phase are mixed to obtain the cream.

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