Antibacterial Compositions Containing Lysozyme and Zinc Hyaluronic Acid and Their Applications

Abstract

This invention discloses an antibacterial composition containing lysozyme and zinc hyaluronic acid and its application, wherein the mass ratio of the active ingredients lysozyme and zinc hyaluronic acid is 0.5-30:1. This invention combines zinc hyaluronic acid and lysozyme to achieve a synergistic antibacterial effect, simultaneously killing bacteria, Candida albicans, and Aspergillus niger, with a particularly significant effect on Aspergillus niger. It also possesses excellent preservative properties and can be used as a preservative in cosmetics and pharmaceuticals, serving as a good natural preservative that can replace chemical preservatives. Furthermore, zinc hyaluronic acid has moisturizing properties, providing both safe preservation and skin stabilization, promoting skin health. When used as a cosmetic preservative, it can replace chemical preservatives, while also offering moisturizing and anti-inflammatory effects with improved safety.

Description

TECHNICAL FIELD

[0001] The present application relates to a bacteriostatic composition, and relates to a bacteriostatic composition containing lysozyme and zinc hyaluronate and application thereof. BACKGROUND

[0002] Lysozyme, also known as muramidase or N-acetylmuramide glycanohydrase, is a natural preservative that exists widely in egg white of poultry and birds, tear fluid, saliva, plasma, urine and other parts of mammals. Lysozyme is a basic globulin composed of a polypeptide chain, and four disulfide bonds are cross-linked in the molecule. Lysozyme can specifically hydrolyze the β-1, 4 glycosidic bond between N-acetylglucosamine (NAG) and N-acetylmuramic acid (NAM) of peptidoglycan molecules. Peptidoglycan is the main component of the cell wall of Gram-positive bacteria. When peptidoglycan is hydrolyzed, the bacterial cell wall is destroyed, the bacterial cell membrane is ruptured under the action of internal osmotic pressure, the contents flow out, and finally the bacteria die. Compared with chemically synthesized preservatives, lysozyme has the advantages of low dose, high efficiency, no irritation, no toxic effect, high stability, etc. In 1992, the Food Additives Association of FAO / WTO recognized that the application of lysozyme in food is safe, so it is widely used in food, cosmetics, medicine and other fields. However, it is worth noting that in the field of cosmetics, chemical preservatives are still the main choice, and the application of lysozyme is not widespread enough. Moreover, lysozyme mainly acts on Gram-positive bacteria and some Gram-negative bacteria, but has little effect on molds.

[0003] Zinc hyaluronate is a zinc salt of hyaluronic acid. In an aqueous solution with a pH value of 6.0-6.5, Zn 2+ can combine with the oxygen-containing donor group in HA to form a zinc hyaluronate (Zn-HA) complex. It has been reported that zinc hyaluronate has bactericidal effect. For example, the article "Comparison of Test Results of Bacteriostatic and Preservative Effect of Zinc Hyaluronate" (Wang Yaohua et al.) confirmed that 0.5% zinc hyaluronate aqueous solution can significantly inhibit Escherichia coli and Candida albicans, and although it also has inhibitory effect on Aspergillus niger, the effect is limited.

[0004] Aspergillus niger is a common species in the genus Aspergillus. In high-temperature and high-humidity environments, Aspergillus niger can grow and reproduce in large quantities to produce enzymes and heat, causing flower drop or boll rot when it infects cotton, or causing rot or soft rot when it grows on juicy fruits, and it can also grow a large amount of black powder on the surface of onion scales, and cause clothes to mildew in plum rain season, etc.

[0005] Traditional preservatives have limited effect on mold killing and high addition amount may cause harm to the human body. For example, hydantoin total formaldehyde content is 17%, free formaldehyde content is 1%, and the mold antibacterial property is poor, and the released formaldehyde has potential carcinogenic risk; methyl chloroisothiazolinone and methyl isothiazolinone have inhibitory effect on yeast and mold, but since this type of preservative has skin irritation, the maximum addition concentration is only 0.01%. The popular polyols and p-hydroxyacetophenone without preservative claims on the market need to be compounded or have high addition amount to have effect on aspergillus niger. SUMMARY

[0006] In view of the deficiencies of the current preservatives in inhibiting aspergillus niger, the present application provides an antibacterial composition, which is compounded by lysozyme and zinc hyaluronate, can achieve the effect of killing multiple bacteria and fungi at the same time, and experiments have proved that the composition can kill common bacteria and fungi such as escherichia coli, staphylococcus aureus, pseudomonas aeruginosa, candida albicans and aspergillus niger, and can be used as a preservative in pharmaceuticals and cosmetics, so that the preservative effect is more convenient and safe, and it is a safe preservative substitute.

[0007] The specific technical scheme of the present application is as follows:

[0008] An antibacterial composition, the effective components include lysozyme and zinc hyaluronate, the mass ratio of lysozyme and zinc hyaluronate is 0.5-30:1, for example, 0.5:1, 0.7:1, 1.0:1, 2:1, 3:1, 4:1, 5:1, 6:1, 7:1, 8:1, 9:1, 10:1, 12:1, 15:1, 20:1, 22:1, 25:1, 28:1, 30:1, preferably, the mass ratio of lysozyme and zinc hyaluronate is 0.7-15:1.

[0009] Further, the molecular weight of the zinc hyaluronate is 10kDa-1000kDa, for example, 10kDa, 30kDa, 36kDa, 40kDa, 80kDa, 100kDa, 150kDa, 200kDa, 250kDa, 300kDa, 350kDa, 400kDa, 500kDa, 600kDa, 700kDa, 800kDa, 900kDa, 1000kDa.

[0010] Further, the zinc hyaluronate can be obtained by ion exchange reaction of zinc ions and sodium hyaluronate.

[0011] Further, lysozyme is a natural anti-infective substance with bactericidal effect, which can act on microbial cell walls and destroy bacterial cell walls, so as to make bacteria dissolve and necrotize.

[0012] The application also provides a method for preparing the bacteriostatic composition, which comprises the step of mixing the lysozyme and the zinc hyaluronate.

[0013] The bacteriostatic composition prepared by mixing the lysozyme and the zinc hyaluronate has good inhibitory and killing effects on common fungi and bacteria, especially on Aspergillus niger. Therefore, the application provides the use of the bacteriostatic composition in inhibiting bacteria and fungi, wherein the bacteria include Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa, and the fungi include Candida albicans and Aspergillus niger.

[0014] Further, the bacteriostatic composition has good inhibitory effect on bacteria and fungi when the content or concentration is 0.05-5.0 wt%, for example, 0.05 wt%, 0.1 wt%, 0.5 wt%, 1.0 wt%, 1.5 wt%, 2 wt%, 2.5 wt%, 3 wt%, 3.5 wt%, 4 wt%, 4.5 wt% and 5 wt%, and preferably 0.1-1.0 wt%.

[0015] The application also provides the use of the bacteriostatic composition as a preservative, which can replace traditional chemical preservatives and has good preservative effect on drugs or cosmetics, and has good inhibitory effect on common bacteria and fungi such as Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, Candida albicans and Aspergillus niger.

[0016] Further, when the bacteriostatic composition is used as a preservative, the addition amount in the product is 0.05-5.0 wt%, for example, 0.05 wt%, 0.1 wt%, 0.5 wt%, 1.0 wt%, 1.5 wt%, 2 wt%, 2.5 wt%, 3 wt%, 3.5 wt%, 4 wt%, 4.5 wt% and 5 wt%, and preferably 0.1-1.0 wt%.

[0017] The application also provides a cosmetic or drug comprising a preservative, wherein the preservative is the bacteriostatic composition, and the content of the bacteriostatic composition is 0.05-5.0 wt%, for example, 0.05 wt%, 0.1 wt%, 0.5 wt%, 1.0 wt%, 1.5 wt%, 2 wt%, 2.5 wt%, 3 wt%, 3.5 wt%, 4 wt%, 4.5 wt% and 5 wt%, and preferably 0.1-1.0 wt%.

[0018] The application also provides a bacteriostatic method, which comprises the step of adding the bacteriostatic composition to cosmetics or drugs.

[0019] Further, in the bacteriostatic method, the bacteria include bacteria and / or fungi. The bacteria include Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa, and the fungi include Candida albicans and Aspergillus niger.

[0020] Further, the bacteriostatic composition is simple to prepare, and the lysozyme and zinc hyaluronate can be dissolved in a solvent to prepare a solution for use, or the lysozyme and zinc hyaluronate can be directly added to a corresponding product formula for use. In the process of preparing the lysozyme and zinc hyaluronate solution, the mixing order of the lysozyme, zinc hyaluronate and solvent has no special requirement, the lysozyme and zinc hyaluronate can be first prepared into a solution, and then the solution is mixed, or the lysozyme and zinc hyaluronate can be added together into the solvent and stirred and mixed uniformly, and the solvent is generally water.

[0021] The present application has the following advantages:

[0022] 1. The present application combines zinc hyaluronate and lysozyme, which has a synergistic bacteriostatic effect, can kill bacteria, Candida albicans and Aspergillus niger at the same time, especially has a significant effect on killing Aspergillus niger, has a good preservative effect, and can be used as a preservative in cosmetics and medicines, is a good natural preservative, and can replace the use of chemical preservatives.

[0023] 2. Zinc hyaluronate has a moisturizing effect, can moisturize and stabilize while being safe and preservative, promotes skin health, can replace the use of chemical preservatives when used as a cosmetic preservative, and has a moisturizing and anti-inflammatory effect, and has better safety. DETAILED DESCRIPTION

[0024] The embodiments of the present application will be described in detail below with reference to the examples, which are only used to illustrate the present application, and those skilled in the art will understand that they should not be regarded as limiting the scope of the present application.

[0025] In the following examples, the concentration is mass percentage unless otherwise specified.

[0026] Example 1

[0027] Precisely weigh 0.4 g of lysozyme and 0.6 g of zinc hyaluronate with a molecular weight of 36 kDa, and add 100 ml of purified water to each, and stir uniformly to prepare 0.4% and 0.6% aqueous solutions, respectively. According to the method of dilution by volume, the 0.4% lysozyme solution and the 0.6% zinc hyaluronate solution are mixed and stirred uniformly to prepare a mixed solution of 0.2% lysozyme and 0.3% zinc hyaluronate.

[0028] Example 2

[0029] Precisely weigh 0.6g of lysozyme, 0.4g of zinc hyaluronate with a molecular weight of 36kDa, and add 100ml of purified water to each, stir uniformly, and prepare 0.4%, 0.6% aqueous solutions respectively. According to the method of doubling dilution, mix the 0.4% lysozyme solution and the 0.6% zinc hyaluronate solution, stir uniformly, and prepare a mixed solution of 0.3% lysozyme and 0.2% zinc hyaluronate.

[0030] Example 3

[0031] Precisely weigh 0.8g of lysozyme, 0.2g of zinc hyaluronate with a molecular weight of 36kDa, and add 100ml of purified water to each, stir uniformly, and prepare 0.8%, 0.2% aqueous solutions respectively. According to the method of doubling dilution, mix the 0.8% lysozyme solution and the 0.2% zinc hyaluronate solution, stir uniformly, and prepare a mixed solution of 0.4% lysozyme and 0.1% zinc hyaluronate.

[0032] Example 4

[0033] Precisely weigh 0.5g of lysozyme, 0.5g of zinc hyaluronate with a molecular weight of 36kDa, and add 100ml of purified water to each, stir uniformly, and prepare 0.5%, 0.5% aqueous solutions respectively. According to the method of doubling dilution, mix the 0.5% lysozyme solution and the 0.5% zinc hyaluronate solution, stir uniformly, and prepare a mixed solution of 0.25% lysozyme and 0.25% zinc hyaluronate.

[0034] Example 5

[0035] Precisely weigh 0.834g of lysozyme, 0.166g of zinc hyaluronate with a molecular weight of 36kDa, and add 100ml of purified water to each, stir uniformly, and prepare 0.834%, 0.166% aqueous solutions respectively. According to the method of doubling dilution, mix the 0.834% lysozyme solution and the 0.166% zinc hyaluronate solution, stir uniformly, and prepare a mixed solution of 0.417% lysozyme and 0.083% zinc hyaluronate.

[0036] Example 6

[0037] Precisely weigh 0.858g of lysozyme, 0.142g of zinc hyaluronate with a molecular weight of 36kDa, and add 100ml of purified water to each, stir uniformly, and prepare 0.858%, 0.14% aqueous solutions respectively. According to the method of doubling dilution, mix the 0.858% lysozyme solution and the 0.142% zinc hyaluronate solution, stir uniformly, and prepare a mixed solution of 0.429% lysozyme and 0.071% zinc hyaluronate.

[0038] Example 7

[0039] Precisely weigh 0.888g of lysozyme, 0.112g of zinc hyaluronate with a molecular weight of 36kDa, and add 100ml of purified water to each, stir uniformly, and prepare 0.888% and 0.112% aqueous solutions, respectively. According to the method of dilution by doubling, mix the 0.888% lysozyme solution and the 0.112% zinc hyaluronate solution, stir uniformly, and prepare a mixed solution of 0.444% lysozyme and 0.056% zinc hyaluronate.

[0040] Example 8

[0041] Precisely weigh 0.91g of lysozyme, 0.09g of zinc hyaluronate with a molecular weight of 36kDa, and add 100ml of purified water to each, stir uniformly, and prepare 0.91% and 0.09% aqueous solutions, respectively. According to the method of dilution by doubling, mix the 0.91% lysozyme solution and the 0.09% zinc hyaluronate solution, stir uniformly, and prepare a mixed solution of 0.455% lysozyme and 0.045% zinc hyaluronate.

[0042] Example 9

[0043] Precisely weigh 0.938g of lysozyme, 0.062g of zinc hyaluronate with a molecular weight of 36kDa, and add 100ml of purified water to each, stir uniformly, and prepare 0.938% and 0.062% aqueous solutions, respectively. According to the method of dilution by doubling, mix the 0.938% lysozyme solution and the 0.062% zinc hyaluronate solution, stir uniformly, and prepare a mixed solution of 0.469% lysozyme and 0.031% zinc hyaluronate.

[0044] Example 10

[0045] Precisely weigh 0.952g of lysozyme, 0.048g of zinc hyaluronate with a molecular weight of 36kDa, and add 100ml of purified water to each, stir uniformly, and prepare 0.952% and 0.048% aqueous solutions, respectively. According to the method of dilution by doubling, mix the 0.952% lysozyme solution and the 0.048% zinc hyaluronate solution, stir uniformly, and prepare a mixed solution of 0.476% lysozyme and 0.024% zinc hyaluronate.

[0046] Example 11

[0047] Precisely weigh 0.962g of lysozyme, 0.038g of zinc hyaluronate with a molecular weight of 36kDa, and add 100ml of purified water to each, stir uniformly, and prepare 0.962% and 0.038% aqueous solutions, respectively. According to the method of dilution by doubling, mix the 0.962% lysozyme solution and the 0.038% zinc hyaluronate solution, stir uniformly, and prepare a mixed solution of 0.481% lysozyme and 0.019% zinc hyaluronate.

[0048] Example 12

[0049] Precisely weigh 0.968 g of lysozyme, 0.032 g of zinc hyaluronate with a molecular weight of 36 kDa, and add 100 ml of purified water to each, stir uniformly, and prepare 0.968% and 0.032% aqueous solutions, respectively. According to the method of dilution by doubling, mix the 0.968% lysozyme solution and the 0.032% zinc hyaluronate solution, stir uniformly, and prepare a mixed solution of 0.484% lysozyme and 0.016% zinc hyaluronate.

[0050] Example 13

[0051] Precisely weigh 0.8 g of lysozyme, 0.2 g of zinc hyaluronate with a molecular weight of 400 kDa, and add 100 ml of purified water to each, stir uniformly, and prepare 0.8% and 0.2% aqueous solutions, respectively. According to the method of dilution by doubling, mix the 0.8% lysozyme solution and the 0.2% zinc hyaluronate solution, stir uniformly, and prepare a mixed solution of 0.4% lysozyme and 0.1% zinc hyaluronate.

[0052] Example 14

[0053] Precisely weigh 0.8 g of lysozyme, 0.2 g of zinc hyaluronate with a molecular weight of 1000 kDa, and add 100 ml of purified water to each, stir uniformly, and prepare 0.8% and 0.2% aqueous solutions, respectively. According to the method of dilution by doubling, mix the 0.8% lysozyme solution and the 0.2% zinc hyaluronate solution, stir uniformly, and prepare a mixed solution of 0.4% lysozyme and 0.1% zinc hyaluronate.

[0054] Example 15

[0055] Precisely weigh 0.16 g of lysozyme, 0.04 g of zinc hyaluronate with a molecular weight of 36 kDa, and add 100 ml of purified water to each, stir uniformly, and prepare 0.16% and 0.04% aqueous solutions, respectively. According to the method of dilution by doubling, mix the 0.16% lysozyme solution and the 0.04% zinc hyaluronate solution, stir uniformly, and prepare a mixed solution of 0.08% lysozyme and 0.02% zinc hyaluronate.

[0056] Example 16

[0057] Precisely weigh 1.6 g of lysozyme, 0.4 g of zinc hyaluronate with a molecular weight of 36 kDa, and add 100 ml of purified water to each, stir uniformly, and prepare 1.6% and 0.4% aqueous solutions, respectively. According to the method of dilution by doubling, mix the 1.6% lysozyme solution and the 0.4% zinc hyaluronate solution, stir uniformly, and prepare a mixed solution of 0.8% lysozyme and 0.2% zinc hyaluronate.

[0058] Example 17

[0059] Precisely weigh 1.0 g of lysozyme, 1.0 g of zinc hyaluronate with a molecular weight of 36 kDa, and add 100 ml of purified water to each, stir to make 1.0%, 1.0% aqueous solution. According to the method of dilution, mix 1.0% lysozyme solution and 1.0% zinc hyaluronate solution, stir to make 0.5% lysozyme and 0.5% zinc hyaluronate mixed solution.

[0060] Comparative Example 1

[0061] Precisely weigh 0.5 g of lysozyme, add 100 ml of purified water, stir to make 0.5% lysozyme aqueous solution.

[0062] Comparative Example 2

[0063] Precisely weigh 0.5 g of zinc hyaluronate with a molecular weight of 36 kDa, add 100 ml of purified water, stir to make 0.5% zinc hyaluronate aqueous solution.

[0064] Comparative Example 3

[0065] Precisely weigh 1.0 g of lysozyme, add 100 ml of purified water, stir to make 1.0% lysozyme aqueous solution.

[0066] Comparative Example 4

[0067] Precisely weigh 1.0 g of zinc hyaluronate with a molecular weight of 36 kDa, add 100 ml of purified water, stir to make 1.0% zinc hyaluronate aqueous solution.

[0068] Comparative Example 5

[0069] Precisely weigh 0.038 g of lysozyme, 0.962 g of zinc hyaluronate with a molecular weight of 36 kDa, add 100 ml of purified water to each, stir to make 0.038%, 0.962% aqueous solution. According to the method of dilution, mix 0.038% lysozyme solution and 0.962% zinc hyaluronate solution, stir to make 0.019% lysozyme and 0.481% zinc hyaluronate mixed solution.

[0070] Comparative Example 6

[0071] Precisely weigh 0.2 g of lysozyme, 0.8 g of zinc hyaluronate with a molecular weight of 36 kDa, add 100 ml of purified water to each, stir to make 0.2%, 0.8% aqueous solution. According to the method of dilution, mix 0.2% lysozyme solution and 0.8% zinc hyaluronate solution, stir to make 0.1% lysozyme and 0.4% zinc hyaluronate mixed solution.

[0072] Comparative Example 7

[0073] Precisely weigh 0.09 g of lysozyme, 0.91 g of zinc hyaluronate with a molecular weight of 36 kDa, and add 100 ml of purified water respectively, stir uniformly, and prepare 0.09% and 0.91% aqueous solutions respectively. According to the method of dilution by doubling, mix the 0.09% lysozyme solution and the 0.91% zinc hyaluronate solution, stir uniformly, and prepare a mixed solution of 0.045% lysozyme and 0.455% zinc hyaluronate.

[0074] Verification example

[0075] The antibacterial effect of each solution prepared in the above examples and comparative examples was verified, and the basic conditions of the products of each example and comparative example are shown in Table 1 below:

[0076] Table 1

[0077]

[0078]

[0079] The antibacterial experiment method is as follows:

[0080] 20 mL of the solution in the above examples and comparative examples was respectively taken into a sterile bottle, and then 0.2 mL of a bacterial suspension was inoculated into the sterile bottle, so that the final bacterial content in the sample was 1 x 10 5 ~ 1 x 10 6 cfu / mL, and the bacterial suspension was Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Candida albicans and Aspergillus niger. After the sample was inoculated with the bacterial suspension, it was immediately mixed uniformly, and then the inoculated sample was placed in a constant temperature incubator at 25°C for storage. The sample was taken for recovery and determination of viable bacteria at 1 day, 4 days, 7 days, 14 days and 28 days after inoculation. The method for determining the number of viable bacteria was as follows: 1.0 mL of the inoculated sample was taken and added to 9 mL of D / E neutralizing broth, and serial dilution was performed, and the number of viable bacteria was determined by the pour plate method, in which bacteria were cultured at 32.5°C for 3-5 days, and Candida albicans and Aspergillus niger were cultured at 22.5°C for 5-7 days. The log reduction value of each group was calculated according to the number of viable bacteria, and the calculated log reduction value was used to challenge the control count (the inoculation concentration per gram of product at the starting point) and the recovery log value at different time points to determine the log reduction amount. That is, the log reduction value R X = logN0-LogN X , where logN0is the log value of the initial inoculation, and LogN X is the recovery log value at different times. A log reduction value of ≥0 indicates an antibacterial effect, and the greater the log reduction value, the better the antibacterial effect.

[0081] The ISO 11930-2012 standard is as follows:

[0082]

[0083] The experimental results are shown below, with Tables 2-18 being the experimental results for Examples 1-17, and Tables 19-25 being the experimental results for Comparative Examples 1-7.

[0084] Table 2. Test results for Example 1 Anticorrosion Challenge

[0085]

[0086]

[0087] Table 3. Test results for Example 2 Anticorrosion Challenge

[0088]

[0089] Table 4. Test results for Example 3 Anticorrosion Challenge

[0090]

[0091] Table 5. Test results for Example 4 Anticorrosion Challenge

[0092]

[0093] Table 6. Test results for Example 5 Anticorrosion Challenge

[0094]

[0095]

[0096] Table 7. Test results for Example 6 Anticorrosion Challenge

[0097]

[0098] Table 8. Test results for Example 7 Anticorrosion Challenge

[0099]

[0100] Table 9. Test results for Example 8 Anticorrosion Challenge

[0101]

[0102] Table 10. Test results for Example 9 Anticorrosion Challenge

[0103]

[0104]

[0105] Table 11. Test results for Example 10 Anticorrosion Challenge

[0106]

[0107] Table 12. Test results for Example 11 Anticorrosion Challenge

[0108]

[0109] Table 13. Test results for Example 12 Anticorrosion Challenge

[0110]

[0111] Table 14. Test results for Example 13 Anticorrosion Challenge

[0112]

[0113] Table 15. Test results for Example 14 Anticorrosion Challenge

[0114]

[0115] Table 16. Test results for Example 15 Anticorrosion Challenge

[0116]

[0117] Table 17. Test results for Example 16 Anticorrosion Challenge

[0118]

[0119]

[0120] Table 18. Test results for Example 17 Anticorrosion Challenge

[0121]

[0122] Table 19. Test results for Comparative Example 1 Anticorrosion Challenge

[0123]

[0124] Table 20. Test results for Comparative Example 2 Anticorrosion Challenge

[0125]

[0126] Table 21. Test results for Comparative Example 3 Anticorrosion Challenge

[0127]

[0128]

[0129] Table 22. Test results of the preservative challenge of Comparative Example 4

[0130]

[0131] Table 23. Test results of the preservative challenge of Comparative Example 5

[0132]

[0133] Table 24. Test results of the preservative challenge of Comparative Example 6

[0134]

[0135] Table 25. Test results of the preservative challenge of Comparative Example 7

[0136]

[0137]

[0138] According to the results of Tables 2-18, it can be found that the different concentrations of lysozyme and zinc hyaluronate in the examples have strong killing effect on bacteria and fungi, and the highest log reduction value of Aspergillus niger can reach 3.76 within 28 days, reaching the A level standard of ISO11930-2012. The comparative examples have certain killing effect on bacteria and Candida albicans, but the log reduction value of Aspergillus niger is mostly <0, indicating no killing effect.

Claims

1. A bacteriostatic composition characterized by: The bacteriostatic composition comprises lysozyme and zinc hyaluronate, the mass ratio of the lysozyme and the zinc hyaluronate is 0.5-30:1, and the molecular weight of the zinc hyaluronate is 10 kDa-1000 kDa.

2. A process for the preparation of the bacteriostatic composition of claim 1, characterized by: The bacteriostatic composition comprises mixing the lysozyme and the zinc hyaluronate.

3. Use of the bacteriostatic composition according to claim 1 for the preparation of a preservative, characterized in that: The preservative has inhibitory effect on bacteria and / or fungi, the bacteria include Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, and the fungi include Candida albicans and Aspergillus niger.

4. Use according to claim 3, characterized in that: The preservative is used for preparing cosmetics and medicines.

5. A method for inhibiting the growth of bacteria for non-therapeutic purposes, characterized in that: The bacteriostatic composition of claim 1 is added into cosmetics as a preservative, the bacteria include Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, and the fungi include Candida albicans and Aspergillus niger.

6. A cosmetic or a medicine, comprising the bacteriostatic composition of claim 1.

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