A fungicide composition containing chlorothalonil and amino oligosaccharide and its application

Abstract

The application discloses a pesticide fungicide combination for preventing and treating cucumber powdery mildew, mango anthracnose and the like and application thereof, and active ingredients of the pesticide fungicide are chlorothalonil and amino oligosaccharide. The application is prepared by compounding chlorothalonil and amino oligosaccharide, the two can produce a synergistic effect, and then the formed pesticide fungicide has a good fungicidal effect and good activity on cucumber powdery mildew, mango anthracnose and the like. The action mechanisms of the effective components in the fungicide combination are different from each other, the action sites are increased, the control spectrum is expanded, the single selection pressure on the pathogen is reduced, the speed of generating disease resistance is slowed down, the use amount is low, the cost is low, meanwhile, the fungicide combination has low side effects and does not cause influences on crop safety and yield and the like.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of pesticides, in particular to a pesticide fungicide containing mefentrifluconazole and amino oligosaccharide for preventing and treating cucumber powdery mildew, mango anthracnose and the like, and application thereof. BACKGROUND

[0002] Cucumber powdery mildew is one of the main diseases in current cucumber production. Cucumber powdery mildew is commonly known as "white hair disease", and the leaf is the most severely damaged, followed by the petiole and stem. After the petiole and tender stem are infected by powdery mildew, the symptoms are similar to those on the leaf, the lesion is smaller, and the powdery substance is less. Yellow spots appear on the leaf, which then expand into circular or elliptical lesions, and a white powdery mold layer is formed on the surface. Generally, the lower leaves are more than the upper leaves, and the back of the leaf is more than the front. The mold spots are initially separate and then combine into a large mold spot, which can even cover the whole leaf, seriously affecting photosynthesis and causing early senescence, thereby causing loss of cucumber yield; mango is one of the most important tropical fruits, widely planted in tropical and subtropical regions, and China is the second largest mango producer in the world. Mango anthracnose fungus (Colletotrichum Corda) is a globally distributed plant pathogen, and mango anthracnose caused by it is one of the most important diseases on mango. It forms latent infection on unripe mangoes and causes huge economic losses during postharvest storage and transportation; at present, cucumber powdery mildew and mango anthracnose are mainly controlled by chemical agents, but the large-scale use of chemical agents can cause pesticide residues and harm human health, and long-term application of single chemical pesticides can easily cause drug resistance.

[0003] In view of the severity of cucumber powdery mildew, mango anthracnose and the like and the harmfulness of chemical pesticides, the present application is based on this demand, and proposes to use amino oligosaccharide and mefentrifluconazole in the combined compound, which have different action mechanisms and increase the action sites, expand the control spectrum and reduce the single selection pressure on the pathogen, which is beneficial to avoid the occurrence of disease resistance and delay the speed of disease resistance, and has low dosage and low cost. At the same time, the fungicidal composition has low side effects and does not affect the safety and yield of crops.

[0004] Mefentrifluconazole is the first triazole fungicide containing isopropyl alcohol structure, and its chemical name is (2RS)-2-[4-(4-chlorophenoxy)-α,α,α-trifluoro-o-tolyl]-1-(1H-1,2,4-triazol-1-yl)propan-2-ol, CAS number is 1417782-03-6, molecular formula is C 18 H 15ClF3N3O2, relative molecular mass is 397.8, the molecular structure of myclobutanil is shown in formula 1. Myclobutanil is soluble in water and organic solvents, the solubility in water at 20℃ is 0.81 mg / L, it has lower water solubility and lower volatility, and will not enter groundwater through leaching; the solubility in organic solvents acetone, ethyl acetate, xylene and 1,2-dichloroethane is 93.2, 116.2, 8.5, 55.3 mg / L respectively. The melting point of myclobutanil is 126℃, the degradation point is 300℃, it will decompose under boiling conditions, but is not flammable.

[0005]

[0006] Myclobutanil is a new type of isopropyl alcohol triazole fungicide with broad-spectrum, high efficiency, systemic, eradication and protection, belonging to sterol demethylation inhibitor. Unlike widely used triazole fungicides, myclobutanil contains isopropyl alcohol structure, which can reduce pathogen mutation and delay pathogen resistance.

[0007] Oligosaccharins, also known as amino oligosaccharins, are low-toxicity fungicides extracted from microbial metabolism, which can inhibit the growth of some pathogens, affect fungal spore germination, induce morphological variation of mycelium, and change intrasporal biochemistry. Oligosaccharins can stimulate the genes in plants to produce chitinase, glucanase, prion and PR protein with disease resistance, and have cell activation effect, which helps the recovery of damaged plants, promotes root and seedling growth, and enhances the stress resistance and growth and development of crops. Oligosaccharin solution can be widely used for preventing and treating diseases caused by viruses, bacteria and fungi in fruit trees, vegetables, underground rhizomes, tobacco, Chinese herbal medicines and grain and cotton crops.

[0008] Cucumber powdery mildew and mango anthracnose are difficult to prevent and control, and have serious harm. The commonly used control agents such as prochloraz are old agents with long time and high resistance, and have poor control effect. In the published reports, the activity of myclobutanil and amino oligosaccharins on cucumber powdery mildew and mango anthracnose has not been deeply studied, and the combination of myclobutanil and amino oligosaccharins has not been reported. Therefore, the prior art combines different agents to prevent and control diseases, but the effect of the combination of different agents is uncertain, which may have synergistic effect or antagonistic effect. The effect of the combination of different agents on different diseases is also uncertain. SUMMARY

[0009] To solve the technical problems in the background art, the present application provides a pesticide fungicide containing myclobutanil and amino oligosaccharins for preventing and controlling cucumber powdery mildew, mango anthracnose and other diseases.

[0010] The object of the present application is achieved by the following technical solutions.

[0011] The present application discloses a fungicide containing myclobutanil and amino oligosaccharide, wherein the active ingredients of the fungicide are myclobutanil and amino oligosaccharide, and the mass ratio of the myclobutanil to the amino oligosaccharide is 1-50:30-1.

[0012] The mass of the myclobutanil accounts for 1-50% of the mass of the fungicide, and the mass of the amino oligosaccharide accounts for 1-30% of the mass of the fungicide.

[0013] As a preferred technical solution, the mass of the myclobutanil accounts for 18% of the mass of the fungicide, and the mass of the amino oligosaccharide accounts for 6% of the mass of the fungicide.

[0014] As a preferred technical solution, the fungicide further comprises, in percentage by mass, 1-9% of a dispersing agent, 1-5.5% of a wetting agent, 0.1-2.5% of a thickening agent, 0.1-4% of a preservative, 0.9-8% of an antifreezing agent, and the rest is water.

[0015] Compared with the prior art, the present application has the following beneficial effects:

[0016] In the disclosed reports, the activity of myclobutanil and amino oligosaccharide on cucumber powdery mildew and mango anthracnose has not been deeply studied, and the combination of myclobutanil and amino oligosaccharide for preventing and treating cucumber powdery mildew and mango anthracnose has not been reported. The fungicide composition disclosed in the present application contains myclobutanil and amino oligosaccharide, has obvious synergistic effect, can effectively improve the prevention and treatment effect on cucumber powdery mildew, mango anthracnose and other diseases, can reduce the dosage of active ingredients, reduce production cost and environmental pollution. DETAILED DESCRIPTION

[0017] In order to make the object, technical scheme and advantages of the embodiments of the present application more clear, the technical scheme of the embodiments of the present application will be described clearly and completely below. If the specific conditions are not indicated in the embodiments, the conventional conditions or the conditions suggested by the manufacturer are adopted. If the reagents or instruments are not indicated by the manufacturer, they are all conventional products that can be purchased in the market.

[0018] The present application discloses a fungicide containing myclobutanil and amino oligosaccharide, wherein the active ingredients of the fungicide are myclobutanil and amino oligosaccharide, and the mass ratio of the myclobutanil to the amino oligosaccharide is 1-50:30-1.

[0019] The dispersing agent provided by the embodiment of the present application includes, but is not limited to, existing dispersing agents such as sodium lignosulfonate, the wetting agent includes, but is not limited to, existing surfactants such as sodium dodecyl sulfate, the thickening agent includes, but is not limited to, existing thickening agents such as xanthan gum, the preservative includes, but is not limited to, existing preservatives such as sodium benzoate, and the antifreeze agent includes, but is not limited to, existing antifreezes such as ethylene glycol.

[0020] It should be noted that the fungicide provided by the present application is used for preventing and treating diseases of crops, including but not limited to cucumber powdery mildew and mango anthracnose, and the fungicide has better prevention and treatment effect on cucumber powdery mildew and mango anthracnose. In addition, the fungicide does not affect the normal growth of plants. The fungicide is a liquid preparation, preferably a suspension agent. It should be noted that the embodiment of the present application only lists the suspension agent, but it can be understood that other types of liquid preparations are also within the protection scope of the embodiment of the present application.

[0021] The features and performances of the present application are further described in detail below in combination with embodiments.

[0022] Embodiment 1

[0023] The present application provides a fungicidal composition, the effective component of the fungicidal composition is chlorothalonil and amino oligosaccharide, and the mass ratio of chlorothalonil to amino oligosaccharide is 18:10.

[0024] Embodiment 2

[0025] The present application provides a fungicidal composition, the effective component of the fungicidal composition is chlorothalonil and amino oligosaccharide, and the mass ratio of chlorothalonil to amino oligosaccharide is 18:8.

[0026] Embodiment 3

[0027] The present application provides a fungicidal composition, the effective component of the fungicidal composition is chlorothalonil and amino oligosaccharide, and the mass ratio of chlorothalonil to amino oligosaccharide is 18:6.

[0028] Embodiment 4

[0029] The present application provides a fungicidal composition, the effective component of the fungicidal composition is chlorothalonil and amino oligosaccharide, and the mass ratio of chlorothalonil to amino oligosaccharide is 18:4.

[0030] Embodiment 5

[0031] The present application provides a fungicidal composition, the effective component of the fungicidal composition is chlorothalonil and amino oligosaccharide, and the mass ratio of chlorothalonil to amino oligosaccharide is 18:2.

[0032] Example 6

[0033] This example provides a fungicidal composition, the ingredients of which are as follows in Table 1 in percentage by mass:

[0034] Table 1 Amount of each component in the fungicidal composition

[0035] Ingredient Name Content (%) Chlorothalonil 18 Amino oligosaccharide 10 Sodium lignosulfonate 1.9 Sodium dodecyl sulfate 2.7 Xanthan gum 0.2 Sodium benzoate 0.3 Ethylene glycol 2.7 Water Balance

[0036] Example 7

[0037] This example provides a fungicidal composition, the ingredients of which are as follows in Table 2 in percentage by mass:

[0038] Table 2 Amount of each component in the fungicidal composition

[0039] Ingredient Name Content (%) Chlorothalonil 18 Amino oligosaccharide 8 Sodium lignosulfonate 2.0 Sodium dodecyl sulfate 3.1 Xanthan gum 0.15 Sodium benzoate 0.5 Ethylene glycol 2 Water Balance

[0040] Example 8

[0041] This example provides a fungicidal composition, the ingredients of which are as follows in Table 3 in percentage by mass:

[0042] Table 3 Amount of each component in the fungicidal composition

[0043] Ingredient Name Content (%) Chlorothalonil 18 Amino oligosaccharide 6 Sodium lignosulfonate 2.1 Sodium dodecyl sulfate 3.2 Xanthan gum 0.2 Sodium benzoate 0.6 Ethylene glycol 0.9 Water Balance

[0044] Example 9

[0045] This example provides a fungicidal composition, the ingredients of which are as follows in Table 4 in percentage by mass:

[0046] Table 4 Amount of each component in the fungicidal composition

[0047] Ingredient Name Content (%) Chlorothalonil 18 Amino oligosaccharide 4 Sodium lignosulfonate 2.3 Sodium dodecyl sulfate 3.5 Xanthan gum 0.2 Sodium benzoate 0.5 Ethylene glycol 3.1 Water Balance

[0048] Example 10

[0049] This example provides a fungicidal composition, the ingredients of which are as follows in Table 5 in percentage by mass:

[0050] Table 5 Amount of each component in the fungicidal composition

[0051] Ingredient Name Content (%) Chlorothalonil 18 Amino oligosaccharide 2 Sodium lignosulfonate 3.2 Sodium dodecyl sulfate 2.7 Xanthan gum 0.2 Sodium benzoate 0.4 Ethylene glycol 3.5 Water Balance Ingredient Name Content (%) Chlorothalonil Amino oligosaccharide Sodium lignosulfonate Sodium dodecyl sulfate Xanthan gum Sodium benzoate Ethylene glycol Water Balance Ingredient Name Content (%) Chlorothalonil Amino oligosaccharide Sodium lignosulfonate Sodium dodecyl sulfate Xanthan gum Sodium benzoate Ethylene glycol Water Balance

[0052] Experimental Example 1 Biological activity test

[0053] According to the guidelines for indoor biological determination of pesticides NY / T 1156.11-2008, NY / T 1156.6-2006, the effects of chlorothalonil, amino oligosaccharide and different mass ratios of their mixtures on cucumber powdery mildew were determined to screen the optimal mixing mass ratio of the two fungicides.

[0054] A, Sphaerotheca fuliginea (Schlecht.) Pollacci was provided by Qingdao Agricultural University Plant Pathology Laboratory, and the conidial suspension mother liquor was obtained by washing the mycelium layer on the leaf surface with deionized water. The concentration was adjusted to 1 x 10 5 50 50 B, the test agent was 97% chlorothalonil technical (purchased from BASF Europe) and 85% amino oligosaccharide technical (purchased from Qingdao Zhongda Agricultural Technology Co., Ltd.)

[0056] C, the mixing ratio of chlorothalonil and amino oligosaccharide was 4:1, 3:1, 2:1, 1:1, 1:2, 1:3 and 1:4, and the concentration was shown in Table 6:

[0057] Table 6 Amount of each substance in fungicidal composition

[0058]

[0059] D, toxicity test method

[0060] Select 4-6 leaf, uniform growth of potting cucumber seedlings, according to the treatment dose in Table 6, dissolve and prepare the corresponding concentration solution with acetone. The instrument used is a spray pot with a capacity of 1 kg and a spray hole diameter of 1 mm. Different treatment concentrations of liquid are uniformly sprayed on the leaves of the test cucumbers, and then naturally air-dried. Another set of water spraying treatment is used as a blank control. Each treatment has 5 pots, and each pot has 2 plants. After 24 hours of pesticide treatment, the conidial suspension in A is uniformly sprayed on the cucumber leaves using a throat sprayer (pressure 0.1 MPa) to inoculate the pathogen. After 10-15 days of incubation in the greenhouse, the disease condition is investigated and recorded after the water control is fully diseased. The control effect is calculated using the formula,

[0061] The grading standard is as follows:

[0062] 0: no disease spot;

[0063] 1: disease spot area accounts for less than 5% of the whole leaf area;

[0064] 3: disease spot area accounts for 5%-15% of the whole leaf area;

[0065] 5: disease spot area accounts for 15%-25% of the whole leaf area;

[0066] 7: disease spot area accounts for 25%-50% of the whole leaf area;

[0067] 9: disease spot area accounts for more than 50%-75% of the whole leaf area; ​

[0068] Level 11: The lesion area accounts for more than 75% of the total leaf area;

[0069]

[0070]

[0071] DPS statistical software was used to perform regression analysis on the logarithmic values ​​of treatment concentrations and corresponding inhibition rate probabilities for each single agent and different combination ratios, and toxicity regression curves and EC50 were calculated. 50 The values ​​and correlation coefficients were calculated, and the co-toxicity coefficient (CTC value) of the mixture was calculated according to Sun Yunpei's method.

[0072] The co-toxicity coefficient (CTC value) of the mixture is calculated according to formulas (1), (2), and (3):

[0073]

[0074] Where: ATI—Measured toxicity index of the mixture;

[0075] S—EC of standard bactericides 50 The unit is milligrams per liter (mg / L);

[0076] M—EC of the mixture 50 The unit is milligrams per liter (mg / L).

[0077] TTI = A × P A +B×P B ···········(2)

[0078] Where: TTI—Theoretical toxicity index of the mixture;

[0079] A-A drug toxicity index;

[0080] P A —The percentage content of agent A in the mixture, expressed as a percentage (%);

[0081] B-B drug toxicity index;

[0082] P B —The percentage content of agent B in the mixture, expressed as a percentage (%).

[0083]

[0084] Where: CTC—co-toxicity coefficient; ATI—measured toxicity index of the mixture; TTI—theoretical toxicity index of the mixture.

[0085] The synergistic effect is represented by the CTC (co-toxicity coefficient) of the combined mixture ≥ 120; the antagonistic effect is represented by the CTC ≤ 80; and the additive effect is represented by 80 < CTC < 120.

[0086] E, the detection results are shown in Tables 7 and 8:

[0087] Table 7 Toxicity test results of chlorothalonil, amino oligosaccharide and 7 ratios

[0088]

[0089] Table 8 Joint toxicity test results of chlorothalonil, amino oligosaccharide mixed agent on cucumber powdery mildew

[0090]

[0091] The research shows (Table 8) that the effective inhibition concentration of 7 kinds of mixed agents (chlorothalonil: amino oligosaccharide = 4:1, 3:1, 2:1, 1:1, 1:2, 1:3, 1:4) on cucumber powdery mildew is 0.7832, 0.8466, 1.0852, 1.5306, 2.8259, 4.3017 and 5.2493 mg / L respectively, and the CTC (co-toxicity coefficient) is 141.58, 139.18, 121.23, 112.08, 87.21, 73.29 and 72.15 respectively. It can be seen that the mixed agent of chlorothalonil and amino oligosaccharide with the ratio of 1:3 and 1:4 shows antagonistic effect, 1:1 and 1:2 shows additive effect, and 4:1, 3:1 and 2:1 shows synergistic effect.

[0092] Experimental Example 2 Field efficacy test

[0093] Field efficacy test one: the fungicidal composition prepared by using the application example 8 is used for field control of cucumber powdery mildew.

[0094] 1. Test method

[0095] The test agent is shown in the following Table 9:

[0096] Table 9 Test dosage table of each agent

[0097]

[0098] Jiangmazhuang Village, Qingdao City, Shandong Province (North Latitude 36.052071, East Longitude 119.878151). The test field area was 2 mu, the soil pH value was 6.4, and the organic matter content was 1.8%. The test crop was cucumber, Youliang No. 2, with a plant distance of 30 cm and a row distance of 80 cm. The field management was uniform and consistent, in line with local agricultural production practices, and was transplanted on April 15, 2021. The cultivation management was carried out according to the local scientific cultivation measures. The target of the test was cucumber powdery mildew. The spraying method was conventional spraying method, the first spraying was carried out in the early stage of cucumber powdery mildew (May 25), with an interval of 7 days, and the second spraying was carried out on June 1. A total of 2 times of spraying were used in this test.

[0099] A commonly used storage pressure type manual sprayer (commercially available) was used for liquid spraying, an adjustable conical nozzle was used, the pressure size was 0.3 MPa, the spraying speed was 0.6 liters / minute, and the liquid use amount per hectare was 750 liters; the incidence of cucumber powdery mildew was investigated in the field before the first spraying, and the investigation was carried out 10 days after the last spraying. Random four-point sampling was carried out in each plot, and two whole leaves of each point were investigated. According to the percentage of the lesion area on the leaf to the whole leaf area, the classification was recorded.

[0100] The classification standard is as follows:

[0101] 0 level: no lesion;

[0102] 1 level: lesion area accounts for less than 5% of the whole leaf area;

[0103] 3 level: lesion area accounts for 6%-10% of the whole leaf area;

[0104] 5 level: lesion area accounts for 11%-20% of the whole leaf area;

[0105] 7 level: lesion area accounts for 21%-40% of the whole leaf area;

[0106] 9 level: lesion area accounts for more than 41% of the whole leaf area.

[0107]

[0108]

[0109] 2、Test results

[0110] The control effects of different pesticide treatments on cucumber powdery mildew are shown in the following Table 10.

[0111] Table 10 Control effect of different pesticide treatments on cucumber powdery mildew

[0112]

[0113] According to the above table, the prevention effect investigation results show that the prevention effect of Example 8, the dosage of which is 40 ml / mu, is the best, the prevention effect is 87.74%, and it is significantly better than the prevention effect of the two single agent control agents. Through field observation, the test crops cucumber treated by the above test agents and control agents are safe, and no phytotoxicity symptoms (such as dwarfing, chlorosis, deformity, etc.) are found.

[0114] Field efficacy test two: the fungicidal composition prepared by Example 8 is used for field prevention and treatment of mango anthracnose.

[0115] 1. Test method:

[0116] The test agent is shown in Table 11 below:

[0117] Table 11: Test dosage of each agent

[0118]

[0119] Maozhu Village, Sanya City, Hainan Province (North Latitude 18.758, East Longitude 109.406). The test field area is 2 mu, the soil pH value is 6.5, and the organic matter content is 1.9%. The test crop is mango, the tree age is 5 years, the mango is in the early stage of new shoot growth, the field management is uniform, and it meets the local agricultural production practice. The test target is mango anthracnose. The spraying method is conventional spraying method, the first spraying is carried out at the early stage of mango anthracnose (January 18, 2021), the interval is 7 days, and the second spraying is carried out on January 25, 2021. This test uses drugs for 2 times.

[0120] A commonly used storage pressure type manual sprayer (commercially available) is used for liquid spraying, the adjustable conical nozzle, the pressure size is 0.3 MPa, the spraying speed is 0.6 liters / minute, and the liquid usage per hectare is 900 liters; the incidence of mango anthracnose is investigated in the field before the first spraying, and the investigation is carried out 7 days after the last spraying. 3 plants are investigated in each plot, and 2 branches of each point are investigated according to the east, south, west, north and center of five directions. The total leaf number and the number of leaves at each level are recorded, and the classification is recorded according to the percentage of the lesion area on the leaf to the whole leaf area.

[0121] The classification standard is as follows:

[0122] 0 level: no lesion;

[0123] 1 level: the lesion area accounts for less than 5% of the whole leaf area;

[0124] 3 level: the lesion area accounts for 6%-15% of the whole leaf area;

[0125] 5 level: the lesion area accounts for 16%-25% of the whole leaf area;

[0126] 7 level: the lesion area accounts for 26%-50% of the whole leaf area;

[0127] 9 grade: the area of the lesion is more than 51% of the whole leaf area.

[0128]

[0129]

[0130] 2、Test results

[0131] The control effects of different agents on mango anthracnose are shown in Table 12.

[0132] Table 12 Control effects of different agents on mango anthracnose

[0133]

[0134] According to the above table, the prevention and control investigation results show that the effect of Example 8, the preparation dosage is 1200 times dilution liquid, on the prevention and control of mango anthracnose is the best, the prevention and control effect is 86.70%, which is significantly better than that of two single agent control agents. Through field observation, the above test agents and control agents are safe to the test crops mango, and no phytotoxicity symptoms (such as dwarfing, chlorosis, deformity, etc.) are found.

[0135] The above only describes the preferred embodiments of the present application and is not used to limit the present application. For those skilled in the art, the present application can have various modifications and changes. Any modification, equivalent replacement, improvement, etc. within the spirit and principle of the present application shall be included in the protection scope of the present application.

Claims

1. Use of a pesticide fungicide for powdery mildew of cucumber, characterized in that, The pesticide fungicide comprises chlorothalonil and amino oligosaccharide, and the mass ratio of the chlorothalonil and the amino oligosaccharide is 18:(6-8).

2. Use according to claim 1, characterized in that: The mass of the chlorothalonil accounts for 18% of the mass of the pesticide fungicide; the mass of the amino oligosaccharide accounts for 6-8% of the mass of the pesticide fungicide.

3. Use according to claim 1, characterized in that: The mass of the chlorothalonil accounts for 18% of the mass of the pesticide fungicide; the mass of the amino oligosaccharide accounts for 6% of the mass of the pesticide fungicide.

4. Use according to any one of claims 1 to 3, characterized in that: The dosage form of the pesticide fungicide is a liquid preparation.

5. Use according to claim 4, characterized in that: The pesticide fungicide further comprises, by mass percentage, the following substances: 1-9% dispersant, 1-5.5% humectant, 0.1-2.5% thickening agent, 0.1-4% preservative, 0.9-8% antifreeze, and the balance is water.

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