Use of hemp polysaccharide and compositions thereof in the preparation of a medicament or skin care product for promoting skin injury repair

Abstract

The present application relates to the application of hemp polysaccharide and its composition in the preparation of medicines or skin care products for promoting skin damage repair. Firstly, the present application creatively finds that hemp polysaccharide can significantly promote the proliferation of human primary skin keratinocytes, the proliferation of human skin fibroblasts and the migration of human skin fibroblasts, and further finds that it has significant efficacy in promoting skin damage repair and promoting skin wound healing, which widens the application field of hemp polysaccharide and greatly improves the economic value of industrial hemp. Secondly, the present application creatively combines hemp polysaccharide, fibronectin and sodium hyaluronate to form a new composition, and the hemp polysaccharide, fibronectin and sodium hyaluronate in the composition cooperate with each other, have synergistic efficacy in promoting skin damage repair and promoting skin wound healing, and can be used for preparing medicines or skin care products for promoting skin damage repair.

Description

TECHNICAL FIELD

[0001] The application belongs to the technical field of hemp extract, and relates to a new application of hemp polysaccharide, in particular to application of hemp polysaccharide and a composition thereof in preparation of a medicine or skin care product for promoting skin injury repair. BACKGROUND

[0002] Industrial hemp is a genetically improved hemp variety, and its toxic component tetrahydrocannabinol (THC) is less than 0.3%. After being licensed, it can be legally planted and processed. It is a high economic value crop integrating food, medicine and fiber, and its stem fiber can be used for textiles, building materials and papermaking, and its seeds can be used for food and medicine. It is widely planted in Yunnan, Inner Mongolia, Heilongjiang and other provinces.

[0003] Research shows that the main cannabinoid compounds in hemp plants are tetrahydrocannabinol (THC), cannabinol (CBN), cannabidiol (CBD), cannabigerol (CBG) and cannabichromene (CBC). The first three account for more than 90% of cannabinoid compounds. Current research on industrial hemp mainly focuses on the pharmacological research of cannabinoid compounds and the application of industrial hemp fiber. Cannabinoid compounds are non-water-soluble components, and the extraction process is organic solvent extraction. In the production and extraction process, the water-soluble components of industrial hemp are treated as waste. However, the polysaccharides and other substances contained in the water-soluble components of industrial hemp also have active effects, but there is little research on the activity of hemp polysaccharides in the prior art.

[0004] CN111978417A discloses a method for extracting hemp polysaccharide and its product and application. The extracted hemp polysaccharide has the effects of scavenging DPPH free radicals and antioxidant. In addition, there is no other research on the application of hemp polysaccharide in the prior art.

[0005] Therefore, it is of great significance to conduct in-depth research on the activity of hemp polysaccharide, explore other uses of hemp polysaccharide, broaden the application field of hemp polysaccharide, and thus improve the economic value of industrial hemp. SUMMARY

[0006] In view of the deficiencies of the prior art, the purpose of the present application is to provide a new application of hemp polysaccharide, and specifically to provide application of hemp polysaccharide and a composition thereof in preparation of a medicine or skin care product for promoting skin injury repair.

[0007] To achieve the purpose of the present application, the following technical solutions are adopted:

[0008] In a first aspect, the present application provides application of hemp polysaccharide in preparation of a medicine or skin care product for promoting skin injury repair.

[0009] The present application creatively discovers that the hemp polysaccharide can significantly promote the proliferation of human primary skin keratinocytes, the proliferation of human skin fibroblasts and the migration of human skin fibroblasts, and further discovers that the hemp polysaccharide has significant efficacy in promoting skin damage repair and promoting skin wound healing, thereby widening the application field of the hemp polysaccharide and greatly improving the economic value of industrial hemp.

[0010] Preferably, the dosage form of the medicine includes any one of a solution, an aerosol, a spray, an ointment or a film.

[0011] Preferably, the medicine further includes a pharmaceutically acceptable excipient.

[0012] Preferably, the excipient includes any one of a diluent, a flavoring agent, a binder or a filler or a combination of at least two of them, such as a combination of a binder and a diluent, a combination of a binder and a flavoring agent, a combination of a binder and a filler, etc., and any other combination mode can be selected, which will not be described one by one here.

[0013] Preferably, the skin care product includes any one of a cosmetic water, an essence water, an essence liquid, a cream, a lotion or a mask.

[0014] Preferably, the composition of the skin care product further includes an additive.

[0015] Preferably, the additive includes any one of an emulsifier, a softening agent, a moisturizing agent, an anti-allergic agent, an antioxidant, a preservative or a pH adjusting agent or a combination of at least two of them, such as a combination of an emulsifier and a softening agent, a combination of a moisturizing agent and an anti-allergic agent, a combination of an anti-allergic agent and an antioxidant, etc., and any other combination mode can be selected, which will not be described one by one here.

[0016] In the present application, the hemp polysaccharide is prepared by a preparation method including the following steps:

[0017] (1) mixing industrial hemp flower leaf raw materials with water, adjusting pH to 3.0-3.5 by adding acid, refluxing for 1-3 times, each time for 1-3 h, combining the extract, and obtaining hemp polysaccharide primary extract;

[0018] (2) purifying the hemp polysaccharide primary extract obtained in step (1) by passing through a D900 column of anion exchange resin, eluting with water, collecting the effluent, and spray drying to obtain the hemp polysaccharide;

[0019] The volume of the water in step (1) is 5-10L, and the acid is one or a combination of at least two of formic acid, acetic acid, oxalic acid, citric acid, hydrochloric acid, nitric acid or sulfuric acid; the mass of the resin in step (2) is 1 / 30-1 / 100 of the mass of the industrial hemp leaf material.

[0020] The specific value in the above 3.0-3.5 can be 3.0, 3.05, 3.1, 3.15, 3.2, 3.25, 3.3, 3.35, 3.4, 3.45, 3.5, etc.

[0021] The specific value in the above 1-3h can be 1h, 1.2h, 1.4h, 1.6h, 1.8h, 2h, 2.2h, 2.4h, 2.6h, 2.8h, 3h, etc.

[0022] The specific value in the above 5-10L can be 5L, 5.5L, 6L, 6.5L, 7L, 7.5L, 8L, 8.5L, 9L, 9.5L, 10L, etc.

[0023] The specific value in the above 1 / 30-1 / 100 can be 1 / 30, 1 / 35, 1 / 40, 1 / 45, 1 / 50, 1 / 55, 1 / 60, 1 / 65, 1 / 70, 1 / 75, 1 / 80, 1 / 85, 1 / 90, 1 / 95, 1 / 100, etc.

[0024] Other specific point values within the above respective numerical ranges can be arbitrarily selected, which will not be described here one by one.

[0025] The hemp polysaccharide preparation method provided by the present application is simple, wherein adjusting the pH to 3.0-3.5 by adding acid can increase the permeability of the cell wall of the plant, facilitate the leaching of polysaccharide, and improve the yield of hemp polysaccharide. The hemp polysaccharide prepared by the preparation method provided by the present application can be used to prepare drugs or skin care products for promoting skin damage repair.

[0026] In a second aspect, the present application provides the use of hemp polysaccharide in the preparation of a skin keratinocyte proliferation promoter.

[0027] The present application creatively finds that hemp polysaccharide can significantly promote the proliferation of human primary skin keratinocytes. There is a layer of keratin protective layer in the epidermis of human skin, which is composed of skin keratinocytes, and its function is to prevent excessive loss of skin moisture and prevent the skin from being damaged during normal friction. Therefore, this finding of the present application can be further applied to the research of related mechanisms such as promoting skin metabolism, protecting skin, protecting skin from damage, promoting skin wound healing, preventing skin moisture loss, etc., and the research and development of related products.

[0028] In a third aspect, the present application provides use of the hemp polysaccharide in the preparation of a skin fibroblast proliferation promoter.

[0029] The present application has found that the hemp polysaccharide can significantly promote the proliferation of human primary skin fibroblasts. The human skin fibroblasts have the function of repairing damaged skin and reducing the influence of age aging on the skin in the human body. Therefore, the present application can be further applied to the research of related mechanisms such as promoting skin metabolism, promoting skin wound healing, preventing skin moisture loss, and the research and development of related products.

[0030] In a fourth aspect, the present application provides use of the hemp polysaccharide in the preparation of a skin fibroblast migration promoter.

[0031] The present application has found that the hemp polysaccharide can significantly promote the migration of human primary skin fibroblasts. The human skin fibroblasts have the function of repairing damaged skin and reducing the influence of age aging on the skin in the human body. Therefore, the present application can be further applied to the research of related mechanisms such as promoting skin metabolism, promoting skin wound healing, preventing skin moisture loss, and the research and development of related products.

[0032] In a fifth aspect, the present application provides use of a hemp polysaccharide composition in the preparation of a medicine or skin care product for promoting skin damage repair, wherein the hemp polysaccharide composition comprises fibronectin, sodium hyaluronate and hemp polysaccharide.

[0033] The present application has found that the hemp polysaccharide can significantly promote the migration of human primary skin fibroblasts. The human skin fibroblasts have the function of repairing damaged skin and reducing the influence of age aging on the skin in the human body. Therefore, the present application can be further applied to the research of related mechanisms such as promoting skin metabolism, promoting skin wound healing, preventing skin moisture loss, and the research and development of related products.

[0034] Preferably, the polysaccharide composition comprises 1-5 parts of polysaccharide, 3-15 parts of fibronectin and 5-20 parts of sodium hyaluronate.

[0035] The specific value in the above 1-5 parts can be selected from 1 part, 1.2 parts, 1.5 parts, 1.7 parts, 2 parts, 2.2 parts, 2.5 parts, 2.7 parts, 3 parts, 3.2 parts, 3.5 parts, 3.7 parts, 4 parts, 4.2 parts, 4.5 parts, 4.7 parts, 5 parts, etc.

[0036] The specific value in the above 3-15 parts can be selected from 3 parts, 4 parts, 5 parts, 6 parts, 7 parts, 8 parts, 9 parts, 10 parts, 11 parts, 12 parts, 13 parts, 14 parts, 15 parts, etc.

[0037] The specific value in the above 5-20 parts can be selected from 5 parts, 6 parts, 7 parts, 8 parts, 9 parts, 10 parts, 11 parts, 12 parts, 13 parts, 14 parts, 15 parts, 16 parts, 17 parts, 18 parts, 19 parts, 20 parts, etc.

[0038] Other specific point values in the above respective numerical ranges can be selected at will, which will not be described here one by one.

[0039] Preferably, the dosage form of the medicine comprises any one of a solution, an aerosol, a spray, an ointment or a film.

[0040] Preferably, the skin care product comprises any one of a cosmetic water, an essence, a serum, a cream, a lotion or a mask.

[0041] Compared with the prior art, the present application has the following beneficial effects:

[0042] Firstly, the present application creatively finds that the polysaccharide can significantly promote the proliferation of human primary skin keratinocytes, the proliferation of human skin fibroblasts and the migration of human skin fibroblasts, and further finds that it has significant efficacy in promoting skin damage repair and promoting skin wound healing, thereby widening the application field of the polysaccharide and greatly improving the economic value of industrial hemp. Secondly, the present application creatively combines the polysaccharide, fibronectin and sodium hyaluronate to form a brand new composition. The polysaccharide, fibronectin and sodium hyaluronate in the composition cooperate with each other and have synergistic efficacy in promoting skin damage repair and promoting skin wound healing, and can be used for preparing a medicine or a skin care product for promoting skin damage repair. DETAILED DESCRIPTION

[0043] In order to further illustrate the technical means adopted by the present application and its effects, the technical scheme of the present application will be further described below in combination with the preferred preparation examples of the present application, but the present application is not limited in the scope of the preparation examples.

[0044] The raw material information involved in the following preparation examples and comparative preparation examples is as follows: human primary skin keratinocytes were purchased from Promocell Company; human primary skin fibroblasts and culture medium were purchased from Sciencell Company, USA; ordinary 96-well plates and 12-well plates were purchased from Corning; CCK-8 cell viability detection kit was purchased from Japan Tongren Biology; fibronectin (model number 43243) was purchased from Guangzhou Qidian Biology Technology Co., Ltd.

[0045] Preparation Example 1

[0046] The present preparation example provides a preparation method of cannabis polysaccharide, which comprises the following steps:

[0047] (1) 10 kg of industrial cannabis leaf raw material was mixed with 50 L of water, acetic acid was added to adjust the pH to 3.0, and reflux extraction was performed for 3 times, each time for 1 h, the extract was combined, and cannabis polysaccharide primary extract was obtained;

[0048] (2) The cannabis polysaccharide primary extract obtained in step (1) was subjected to anion exchange resin D900 column chromatography (resin amount 350 g), and eluted with water until the effluent was nearly colorless, the effluent was collected, and spray dried to obtain 758.09 g of the cannabis polysaccharide. The obtained cannabis polysaccharide was detected for polysaccharide content by phenol-sulfuric acid method, and the polysaccharide content was 18.78%.

[0049] Preparation Example 2

[0050] The present preparation example provides a preparation method of cannabis polysaccharide, which comprises the following steps:

[0051] (1) 10 kg of industrial cannabis leaf raw material was mixed with 100 L of water, formic acid was added to adjust the pH to 3.5, and reflux extraction was performed for 1 time, the reflux time was 3 h, the extract was collected, and cannabis polysaccharide primary extract was obtained;

[0052] (2) The cannabis polysaccharide primary extract obtained in step (1) was subjected to anion exchange resin D900 column chromatography (resin amount 100 g), and eluted with water until the effluent was nearly colorless, the effluent was collected, and spray dried to obtain 560.67 g of the cannabis polysaccharide. The obtained cannabis polysaccharide was detected for polysaccharide content by phenol-sulfuric acid method, and the polysaccharide content was 24.35%.

[0053] Preparation Example 3

[0054] The present preparation example provides a preparation method of cannabis polysaccharide, which comprises the following steps:

[0055] (1) 10 kg of industrial cannabis leaf raw material was mixed with 80 L of water, oxalic acid was added to adjust the pH to 3.2, and reflux extraction was performed for 2 times, each time for 2 h, the extract was combined, and cannabis polysaccharide primary extract was obtained;

[0056] (2) The hemp polysaccharide primary extract obtained in step (1) is subjected to column chromatography on a D900 anion exchange resin (resin amount 500 g), and eluted with water until the effluent is nearly colorless. The effluent is collected and spray dried to obtain 630.24 g of the hemp polysaccharide. The hemp polysaccharide obtained is subjected to polysaccharide content detection by the phenol-sulfuric acid method, and the polysaccharide content is 22.89%.

[0057] Preparation Example 4

[0058] The present preparation example provides a hemp polysaccharide composition, which comprises 2 parts of hemp polysaccharide, 8 parts of fibronectin, and 10 parts of sodium hyaluronate by weight.

[0059] The preparation method of the hemp polysaccharide is the same as that in Preparation Example 1.

[0060] The preparation method of the hemp polysaccharide composition only needs to simply physically mix the components uniformly.

[0061] Preparation Example 5

[0062] The present application example provides a hemp polysaccharide composition, which comprises 2 parts of hemp polysaccharide, 4 parts of fibronectin, and 14 parts of sodium hyaluronate by weight.

[0063] The preparation method of the hemp polysaccharide is the same as that in Preparation Example 2.

[0064] The preparation method of the hemp polysaccharide composition refers to Preparation Example 4.

[0065] Preparation Example 6

[0066] The present preparation example provides a hemp polysaccharide composition, which comprises 2 parts of hemp polysaccharide, 4 parts of fibronectin, and 14 parts of sodium hyaluronate by weight.

[0067] The preparation method of the hemp polysaccharide is the same as that in Preparation Example 3.

[0068] The preparation method of the hemp polysaccharide composition refers to Preparation Example 4.

[0069] Comparative Preparation Example 1

[0070] The present comparative preparation example provides a single fibronectin component.

[0071] Comparative Preparation Example 2

[0072] The present comparative preparation example provides a single sodium hyaluronate component.

[0073] Comparative Preparation Example 3

[0074] This comparative preparation example provides a hemp polysaccharide composition, which is different from Preparation Example 4 only in that "hemp polysaccharide 2 parts, fibronectin 8 parts and sodium hyaluronate 10 parts" is replaced by "a combination of hemp polysaccharide and fibronectin 20 parts" wherein the mass ratio of hemp polysaccharide to fibronectin is 1:4.

[0075] The preparation method thereof refers to Preparation Example 4.

[0076] Comparative Preparation Example 4

[0077] This comparative preparation example provides a hemp polysaccharide composition, which is different from Preparation Example 4 only in that "hemp polysaccharide 2 parts, fibronectin 8 parts and sodium hyaluronate 10 parts" is replaced by "a combination of hemp polysaccharide and sodium hyaluronate 20 parts" wherein the mass ratio of hemp polysaccharide to sodium hyaluronate is 1:5.

[0078] The preparation method thereof refers to Preparation Example 4.

[0079] Comparative Preparation Example 5

[0080] This comparative preparation example provides a composition, which is different from Preparation Example 4 only in that "hemp polysaccharide 2 parts, fibronectin 8 parts and sodium hyaluronate 10 parts" is replaced by "a combination of fibronectin and sodium hyaluronate 20 parts" wherein the mass ratio of fibronectin to sodium hyaluronate is 4:5.

[0081] The preparation method thereof refers to Preparation Example 4.

[0082] Test Example 1

[0083] Evaluation of the proliferation-promoting effect of hemp polysaccharide on human primary skin keratinocytes

[0084] Test sample: hemp polysaccharide prepared in Preparation Examples 1-3, and the test method is as follows:

[0085] Human primary skin keratinocytes were routinely cultured in a culture medium specially for human primary skin keratinocytes under a culture condition containing 5% carbon dioxide at 37°C. When the cells grew to 90% density, the cell suspension density was adjusted to 1x10 5 After adhering for 48h, different concentrations of the test sample were added to the cell suspension of each experimental group for 24h, and no test sample was added to the cell suspension of the blank group. Subsequently, according to the operation in the CCK-8 kit instruction, CCK-8 reagent was added for incubation for 2h, and the absorbance was measured at 450nm.

[0086]

[0087] The results are shown in Table 1:

[0088] Table 1

[0089]

[0090]

[0091] The results showed that the cannabis polysaccharides prepared in Examples 1-3 could significantly enhance the activity of human primary skin keratinocytes, thus indicating that they have a significant effect on promoting the proliferation of human primary skin keratinocytes. Moreover, the proliferation-promoting effect of cannabis polysaccharides on human primary skin keratinocytes is significantly concentration-dependent.

[0092] Test Example 2

[0093] Evaluation of the proliferative effect of cannabis polysaccharides on human primary skin fibroblasts

[0094] Test sample: Cannabis polysaccharides prepared in Examples 1-3, and the test methods are as follows:

[0095] Human primary skin fibroblasts were cultured using ScienCell medium at 37°C and in a 5% carbon dioxide atmosphere. When the cells reached 90% confluence, they were cultured at a rate of 2 × 10⁶ cells / year. 4 Cells were seeded at a density of [number] cells / ml in 96-well plates. After 48 hours of adhesion, different concentrations of the test sample were added to the cell suspension in each experimental group for 24 hours of treatment. No test sample was added to the cell suspension in the control group. Subsequently, the CCK-8 reagent was added and incubated for 2 hours according to the CCK-8 kit instructions, and the absorbance was measured at 450 nm.

[0096]

[0097] The results are shown in Table 2:

[0098] Table 2

[0099]

[0100]

[0101] The results showed that the cannabis polysaccharides prepared in Examples 1-3 could significantly enhance the activity of human primary skin fibroblasts, thus indicating that they have a significant effect on promoting the proliferation of human primary skin fibroblasts. Moreover, the proliferation-promoting effect of cannabis polysaccharides on human primary skin fibroblasts is significantly concentration-dependent.

[0102] Test Example 3

[0103] Evaluation of the migration-promoting effects of cannabis polysaccharides and their compositions on human primary dermal fibroblasts

[0104] Tested samples: the hemp polysaccharide of Preparation Example 1, the compositions of Preparation Examples 4-6, the single components of Comparative Preparation Examples 1 and 2, the compositions of Comparative Preparation Examples 3-5, and the test method is as follows:

[0105] Human primary skin fibroblasts were routinely cultured in a culture condition containing 5% carbon dioxide at 37°C with complete medium, and after the completion of the culture, the cell suspension density was adjusted to 1x10 5 After 48h of adhesion, a scratch was made on the cell layer with a needle, and the cell debris was washed with PBS. The tested samples were added to the cell suspension in each experimental group to make the concentration in the system 200 μg / mL. 0.02 μg / mL TGF-β was added to the cell suspension in the control group as a positive control, and no tested sample was added to the cell suspension in the blank group. After 24h, the healing of the scratch on the cell layer was observed.

[0106]

[0107] Table 3

[0108] Group Healing rate (%) Blank group 45.13 Control group 62.29 Preparation Example 1 66.21 Preparation Example 4 69.21 Preparation Example 5 69.46 Preparation Example 6 69.79 Comparative Preparation Example 1 38.03 Comparative Preparation Example 2 31.11 Comparative Preparation Example 3 67.26 Comparative Preparation Example 4 67.83 Comparative Preparation Example 5 44.51

[0109] The results show that the hemp polysaccharide of Preparation Example 1, the hemp polysaccharide compositions of Preparation Examples 4-6, and the hemp polysaccharide compositions of Comparative Preparation Examples 3 and 4 have a significant effect of promoting the migration of human primary skin fibroblasts. The hemp polysaccharide compositions of Preparation Examples 4-6 have a better effect of promoting the migration of human primary skin fibroblasts than the hemp polysaccharide single component of Preparation Example 1. The single components of Comparative Preparation Examples 1 and 2 and the composition of Comparative Preparation Example 5 have a poor effect of promoting the migration of human primary skin fibroblasts.

[0110] Test Example 4

[0111] Evaluation of the effect of hemp polysaccharide and its composition on promoting skin wound healing

[0112] The hemp polysaccharide of Preparation Example 1, the hemp polysaccharide compositions of Preparation Examples 4-6, the single component of Comparative Preparation Example 1, the single component of Comparative Preparation Example 2, and the hemp polysaccharide compositions of Comparative Preparation Examples 3-5 were taken as the tested samples, and were dissolved in sterile normal saline to make a 1 mg / mL tested sample solution. The effect of the tested samples on promoting skin wound healing was tested, and the test method is as follows:

[0113] (1) Mouse model:

[0114] A mouse full-thickness skin wound model was established: 5-6-week-old male mice were raised according to the animal care and handling procedures, anesthetized by intraperitoneal injection of 350 mg / kg chloral hydrate, the back skin was disinfected, a circular hole with a diameter of 8 mm was punched on the back of the mouse with a punch, and full-thickness skin was cut to the fascia. Each animal was housed in a separate cage. A total of 40 experimental mice were randomly divided into 10 groups. The second to tenth groups were applied with equal amounts of sample solutions prepared in Preparation Examples 1, 4-6, and Comparative Preparation Examples 1-5 at the wound site, respectively. The first group was the control group, and equal amounts of sterile normal saline were applied at the wound site. Each group was applied once in the morning and once in the evening, and the application was continued for 10 days. The wound surface was photographed at the same fixed height on the 0th and 10th days, and the wound healing rate was calculated according to the following formula:

[0115]

[0116] The results are shown in Table 4.

[0117] Table 4

[0118]

[0119]

[0120] (2) Human trials:

[0121] A total of 100 social volunteers with skin wounds on the legs or arms and similar wound sizes were selected, aged 20-30 years old, and randomly divided into 10 groups, 10 people in each group. Each person in the second to tenth groups applied equal amounts of sample solutions prepared in Preparation Examples 1, 4-6, and Comparative Preparation Examples 1-5 at the wound site every day (once in the morning and once in the evening). The first group was the control group, and equal amounts of sterile normal saline were applied at the wound site. After one week of testing, the wound healing of each group of volunteers was compared (no other drugs or reagents for promoting wound healing were given to each group during the test period). The results are shown in Table 5.

[0122] Table 5

[0123]

[0124]

[0125] The results show that the hemp polysaccharide of Preparation Example 1, the hemp polysaccharide compositions of Preparation Examples 4-6, and Comparative Preparation Examples 3 and 4 have a significant effect on promoting skin wound healing. The hemp polysaccharide compositions of Preparation Examples 4-6 have a better effect on promoting skin wound healing than the single component of the hemp polysaccharide of Preparation Example 1. The single components of Comparative Preparation Examples 1 and 2 and the composition of Comparative Preparation Example 5 have a poor effect on promoting skin wound healing.

[0126] In summary, firstly, the application creatively finds that the hemp polysaccharide can significantly promote the proliferation of human primary skin keratinocytes, the proliferation of human skin fibroblasts and the migration of human skin fibroblasts, and further finds that it has significant efficacy in promoting skin damage repair and promoting skin wound healing, thereby widening the application field of the hemp polysaccharide and greatly improving the economic value of industrial hemp. Secondly, the application creatively combines the hemp polysaccharide, fibronectin and sodium hyaluronate to form a new composition, and the hemp polysaccharide, fibronectin and sodium hyaluronate in the composition cooperate with each other and have synergistic efficacy in promoting skin damage repair and promoting skin wound healing, and can be used for preparing a medicine or skin care product for promoting skin damage repair.

[0127] The applicant declares that the application of the hemp polysaccharide and the composition thereof in the preparation of the medicine or skin care product for promoting skin damage repair is illustrated by the above preparation examples, but the application is not limited to the above preparation examples, that is, it does not mean that the application must rely on the above preparation examples to be implemented. It should be understood by those skilled in the art that any improvement on the application, equivalent replacement of each raw material of the product of the application, addition of auxiliary ingredients, selection of specific modes and the like all fall within the protection scope and disclosure scope of the application.

[0128] The preferred embodiments of the application are described in detail above, but the application is not limited to the specific details in the above embodiments, and various simple modifications can be made to the technical solutions of the application within the technical concept of the application, and these simple modifications all belong to the protection scope of the application.

[0129] In addition, it should be noted that each specific technical feature described in the above specific embodiments can be combined by any suitable method without contradiction, and in order to avoid unnecessary repetition, the application will not further describe various possible combination methods.

Claims

1. Use of a Cannabis polysaccharide composition for the manufacture of a medicament for promoting repair of skin damage, characterized in that, The hemp polysaccharide composition comprises hemp polysaccharide 1-5 parts, fibronectin 3-15 parts and sodium hyaluronate 5-20 parts by weight; The hemp polysaccharide is prepared by a preparation method comprising the following steps: (1) mixing industrial hemp leaf raw material with water, adjusting pH to 3.0-3.5 by adding acid, refluxing for 1-3 times, each time for 1-3 hours, combining the extract, and obtaining hemp polysaccharide primary extract; (2) purifying the hemp polysaccharide primary extract obtained in step (1) by passing through a D900 anion exchange resin column, eluting with water, collecting the effluent, and spray drying to obtain the hemp polysaccharide; The volume of the water in step (1) is 5-10 L based on 1 Kg of the mass of the industrial hemp leaf raw material; the acid is any one or a combination of at least two of formic acid, acetic acid, oxalic acid, citric acid, hydrochloric acid, nitric acid or sulfuric acid; the mass of the resin in step (2) is 1 / 30-1 / 100 of the mass of the industrial hemp leaf raw material.

2. Use according to claim 1, wherein The dosage form of the medicine comprises any one of a solution, an aerosol, a spray, an ointment or a film.

3. The use according to claim 1, wherein The medicine further comprises a pharmaceutically acceptable excipient.

4. The use according to claim 3, wherein the compound is ###0002### The excipient comprises any one or a combination of at least two of a diluent, a flavoring agent, a binder or a filler. The excipient comprises any one or a combination of at least two of a diluent, a flavoring agent, a binder or a filler.

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