Adaptive compression assembly, thermostatic unit, variable temperature unit, mixing unit and temperature control module

Abstract

The application discloses a self-adaptive compression assembly, a floating compression constant-temperature unit, a floating compression variable-temperature unit, a floating compression mixing unit and a temperature control module. The self-adaptive compression assembly comprises a mounting seat, a floating compression assembly and an elastic member. The floating compression assembly is used for compressing a reagent tube at a target position. The floating compression assembly is movably arranged on the mounting seat. The floating compression assembly is adapted to compress or release the reagent tube by moving relative to the mounting seat. The elastic member is arranged between the mounting seat and the floating compression assembly. The elastic member is used for adaptively providing an elastic force for driving the floating compression assembly to compress the reagent tube. In the application, the self-adaptive compression assembly can always compress the reagent tube at the target position on the opposite side. The self-adaptive capability can prevent the reagent tube from being stuck or jammed during the whole process. The whole process has little impact on the reagent tube, and is beneficial to the reagent tube to smoothly pass through the corresponding area or the corresponding temperature zone.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of in vitro detection, in particular to a self-adaptive compression assembly, a floating compression constant-temperature unit, a floating compression variable-temperature unit, a floating compression mixing unit and a temperature control module. BACKGROUND

[0002] Polymerase Chain Reaction (PCR) is a method of amplifying specific DNA fragments. During the PCR reaction, the reagent tube needs to be heated to a certain temperature and then cooled to another temperature, and the cycle needs to be repeated more than 40 times. The cycle is repeated many times.

[0003] At present, the common temperature control methods mainly include air bath, water bath and solid heat conduction. The air bath actually heats the air, and the air transfers heat to the reagent tube in the air environment. Because the heat conduction efficiency of air is low, the heating and cooling efficiency is very slow, which affects the detection efficiency. The water bath uses a transmission mechanism to cycle the reagent tube into different temperature water tanks. The water bath has good heat conduction, but the water bath device has a large volume due to the setting of multiple water tanks, and water will vaporize at a high temperature. Therefore, water needs to be added later, which is not convenient for maintenance.

[0004] In essence, the solid heat conduction method is a relatively fast temperature control method. However, if a single solid heat conduction part is used, the solid heat conduction part itself needs a long time to heat up and cool down, which will also affect the temperature control efficiency. In addition, the PCR reaction needs to be repeated more than 40 times at two temperatures, which puts very high requirements on the temperature change efficiency and heat conduction efficiency of the solid heat conduction part. If multiple solid heat conduction parts are used to heat the reagent tube in different temperature zones during the reaction, the reagent tube needs to be fully contacted with the corresponding solid heat conduction part without impacting and damaging the reagent tube, and without causing jamming, stalling and other problems. How to make the reagent in the reagent tube evenly heated is a problem to be solved. SUMMARY

[0005] Therefore, the main purpose of the present application is to provide a self-adaptive compression assembly, a floating compression constant-temperature unit, a floating compression variable-temperature unit, a floating compression mixing unit and a temperature control module, so that the reagent tube can enter and exit each region without impact, and without jamming, stalling and other problems.

[0006] To solve the above problems and problems related to the above problems, the present application provides the following technical solutions:

[0007] A self-adaptive compression assembly comprises:

[0008] mounting base;

[0009] a floating pressing member for pressing a reagent tube to a target position, the floating pressing member being movably arranged on the mounting base, the floating pressing member being adapted to press or release the reagent tube by moving relative to the mounting base; and

[0010] a resilient member arranged between the mounting base and the floating pressing member, the resilient member being adapted to provide a resilient force for driving the floating pressing member to press the reagent tube.

[0011] Optionally, the floating pressing member is provided with a pressing surface for contacting the reagent tube, the floating pressing member being adapted to move under the action of the resilient member when the reagent tube is pressed between the target position and the pressing surface.

[0012] Optionally, the mounting base is provided with a guide slot, the length direction of the guide slot being along the pressing direction, the floating pressing member being provided with a guide portion, the guide portion being movably arranged in the guide slot.

[0013] or

[0014] the floating pressing member is provided with a guide slot, the length direction of the guide slot being along the pressing direction, the mounting base being provided with a guide portion, the guide portion being movably arranged in the guide slot.

[0015] Optionally, the guide portion is a pin shaft, the floating pressing member being adapted to rotate around the axis of the pin shaft, each of the resilient members being arranged on two sides of the pin shaft.

[0016] the floating pressing member being adapted to rotate around the pin shaft under the action of the resilient member when the reagent tube is pressed between the target position and the pressing surface.

[0017] Optionally, the reagent tube has a flattened portion pressed between the target position and the pressing surface, the surface of the flattened portion being a first inclined surface, the pressing surface being a second inclined surface for abutting against the first inclined surface.

[0018] Optionally, the floating pressing member is provided with a chamfered surface for guiding the pressed object to be pressed between the pressing surface and the target position, the two sides of the chamfered surface being provided with transition circular arcs, and one of the transition circular arcs being between the chamfered surface and the pressing surface.

[0019] Optionally, the floating pressing member comprises:

[0020] a first heat-conducting member, the pressing surface being formed on the first heat-conducting member; and

[0021] A first thermal insulation member separates the first thermal conductive member and the mounting base.

[0022] Optionally, the first thermal insulation member comprises a thermal insulation plate and a protrusion provided on the thermal insulation plate, and a pin hole is formed in the protrusion.

[0023] The mounting base comprises a base plate and first and second supporting ears provided on the base plate, and a waist-shaped hole serving as the guide slot is formed in each of the first and second supporting ears.

[0024] The first and second supporting ears are symmetrically distributed on two sides of the protrusion, the pin shaft is provided in the pin hole, and the two ends of the pin shaft correspondingly pass through the waist-shaped holes in the first and second supporting ears.

[0025] Optionally, the floating compression assembly further comprises:

[0026] A heating element is used to heat the first thermal conductive member, and the heating element is in contact with the first thermal conductive member.

[0027] A first temperature acquisition element is used to acquire the temperature of the first thermal conductive member.

[0028] The first temperature acquisition element is directly provided on the first thermal conductive member, or the temperature element is provided between the first thermal insulation member and the first thermal conductive member and is in direct contact with the first thermal conductive member.

[0029] The application further provides a floating compression constant-temperature unit, which comprises two groups of self-adapting compression assemblies, each of the self-adapting compression assemblies is the self-adapting compression assembly with a heating element, the compression surfaces of the two groups of self-adapting compression assemblies face each other and are separated from each other, so that a self-adapting compression space for a reagent tube to be squeezed into or through is formed between the two compression surfaces, and the heating element is used to keep the temperature of the first thermal conductive member at a first target temperature.

[0030] Or

[0031] The floating compression heating element comprises a first self-adaptive compression assembly and a second self-adaptive compression assembly; the first self-adaptive compression assembly is one of the self-adaptive compression assemblies with a heating element, and the heating element is used to keep the temperature of the first heat-conducting member at a first target temperature; the second self-adaptive compression assembly is one of the self-adaptive compression assemblies without a heating element, wherein the compression surface of the first self-adaptive compression assembly faces the compression surface of the second self-adaptive compression assembly and is spaced apart from the compression surface of the second self-adaptive compression assembly, so that an adaptive compression space for the reagent tube to be extruded into or through is formed between the two compression surfaces.

[0032] The application also provides a floating compression variable-temperature unit, comprising:

[0033] a variable-temperature assembly, wherein the variable-temperature assembly comprises a second heat-conducting member and a variable-temperature element used to heat or cool the second heat-conducting member, and the second heat-conducting member is provided with a heat-conducting compression surface used to directly contact the reagent tube;

[0034] a self-adaptive compression assembly, wherein the self-adaptive compression assembly is any one of the self-adaptive compression assemblies described above;

[0035] wherein the heat-conducting compression surface and the compression surface face each other, and a compression space for the reagent tube to be extruded into or through is formed between the heat-conducting compression surface and the compression surface.

[0036] Optionally, the variable-temperature element is a semiconductor refrigeration sheet, and the variable-temperature assembly further comprises a heat dissipation seat, a second heat insulation member, a second temperature acquisition element used to acquire the temperature of the heat dissipation seat, and a third temperature acquisition element used to acquire the temperature of the second heat-conducting member; the second heat insulation member is arranged on the heat dissipation seat and separates the heat dissipation seat from the second heat-conducting member; the second heat insulation member is provided with a hollow mounting area, and the semiconductor refrigeration sheet is arranged in the hollow mounting area; one end of the semiconductor refrigeration sheet is in contact with the heat dissipation seat, and the other end of the semiconductor refrigeration sheet is in contact with the second heat-conducting member.

[0037] Optionally, the heat dissipation seat is provided with a fan.

[0038] The application also provides a floating compression mixing unit, comprising:

[0039] an ultrasonic assembly, wherein the ultrasonic assembly comprises a vibration head and an ultrasonic vibration source used to provide vibration power for the vibration head;

[0040] a self-adaptive compression assembly, wherein the self-adaptive compression assembly is any one of the self-adaptive compression assemblies described above;

[0041] wherein a compression space for the reagent tube to be extruded into or through is formed between the top end of the vibration head and the compression surface of the self-adaptive compression assembly.

[0042] The application further provides a temperature control module, comprising a carrier, wherein one or two or more of a floating compression constant temperature unit, a floating compression variable temperature unit or a floating compression mixing unit is arranged on the carrier, the floating compression constant temperature unit is any one of the floating compression constant temperature units described above, the floating compression variable temperature unit is any one of the floating compression variable temperature units described above, and the floating compression mixing unit is any one of the floating compression mixing units described above.

[0043] Optionally, the carrier and the reagent tube can be relatively moved or rotated, the carrier is provided with a reagent tube variable temperature channel for the reagent tube to pass through, and each unit corresponds to a compression space forming a section of the reagent tube variable temperature channel.

[0044] Optionally, the temperature control module comprises a rack and a driving mechanism for driving the carrier to move relative to the rack, and when the temperature is controlled, the reagent tube is arranged stationary relative to the rack, and the driving mechanism comprises:

[0045] a lead screw arranged on the rack, and the arrangement direction of each unit is consistent with the axial direction of the lead screw;

[0046] a lead screw nut arranged on the carrier, and the lead screw and the lead screw nut are connected in cooperation;

[0047] a power element arranged on the rack, and the power element is used to provide power for rotating the lead screw;

[0048] wherein the power element is connected with a driving wheel, a driven wheel is arranged on the lead screw, and the driving wheel and the driven wheel are connected through a synchronous belt.

[0049] Optionally, the carrier is provided with three floating compression constant temperature units, one floating compression variable temperature unit and one floating compression mixing unit, the three floating compression constant temperature units are a first floating compression constant temperature unit, a second floating compression constant temperature unit and a third floating compression constant temperature unit, respectively, wherein the floating compression mixing unit, the floating compression variable temperature unit, the first floating compression constant temperature unit, the second floating compression constant temperature unit and the third floating compression constant temperature unit are arranged in sequence along the reagent tube variable temperature channel,

[0050] wherein the floating compression mixing unit is used to mix the reagent in the reagent tube A; the second heat conduction element of the floating compression variable temperature unit is kept at a temperature lower than a target low temperature in a preset time period; the first heat conduction element of the first floating compression constant temperature unit is kept at the target low temperature; the first heat conduction element of the second floating compression constant temperature unit is kept at a temperature higher than a target high temperature; and the first heat conduction element of the third floating compression constant temperature unit is kept at the target high temperature.

[0051] In the application, the adaptive compression assembly can always compress the reagent tube to the target position on the opposite side, and the adaptive ability can prevent the whole process from being stuck or jammed, and the whole process has little impact on the reagent tube, which is beneficial to the smooth passing of the reagent tube through the corresponding area or the corresponding temperature zone. BRIEF DESCRIPTION OF DRAWINGS

[0052] Figure 1 An exemplary structural schematic diagram of the temperature control module of the application is shown.

[0053] Figure 2 An exemplary structural schematic diagram of the temperature control module of the application is shown. Figure 1 An exemplary structural schematic diagram of the temperature control module of the application is shown.

[0054] Figure 3 An exemplary structural schematic diagram of the adaptive compression unit of the application is shown. An exemplary structural schematic diagram of the adaptive compression unit of the application is shown.

[0055] An exemplary structural schematic diagram of the adaptive compression unit of the application is shown. Figure 4 An exemplary structural schematic diagram of the adaptive compression unit of the application is shown. Figure 2 An exemplary structural schematic diagram of the adaptive compression unit of the application is shown. An exemplary structural schematic diagram of the adaptive compression unit of the application is shown.

[0056] An exemplary structural schematic diagram of the adaptive compression unit of the application is shown. Figure 5 An exemplary structural schematic diagram of the adaptive compression unit of the application is shown. An exemplary structural schematic diagram of the adaptive compression unit of the application is shown.

[0057] An exemplary structural schematic diagram of the adaptive compression unit of the application is shown. Figure 6 An exemplary structural schematic diagram of the adaptive compression unit of the application is shown. Figure 2 An exemplary structural schematic diagram of the adaptive compression unit of the application is shown.

[0058] PART NUMBER EXPLANATION:

[0059] Reagent tube A;

[0060] Adaptive compression assembly 100, mounting seat 110, base plate 110a, first supporting lug 110b, guide groove 110c, floating compression assembly 120, compression surface 120a, chamfer surface 120c, elastic member 130, pin shaft 140, spacing space 101;

[0061] Heating element 221, first heat conduction member 222, groove 222a, first heat insulation member 224, heat insulation plate 224a, protrusion 224b;

[0062] Temperature changing assembly 300, second heat conduction member 310, heat conduction compression surface 311, temperature changing element 320, heat dissipation seat 330, second heat insulation member 340, hollow mounting area 341, fan 350, second temperature acquisition element 360, third temperature acquisition element 370;

[0063] Vibration source 410, vibration head 420;

[0064] Carrying member 500, reagent tube temperature changing channel 501;

[0065] screw 610, screw nut 620, power element 630, driving wheel 640, driven wheel 650;

[0066] frame 700. DETAILED DESCRIPTION

[0067] The following embodiments of the application are illustrated by way of specific examples, and other advantages and effects of the present application will be readily appreciated by those skilled in the art from the following description.

[0068] The adaptive compression assembly of the following embodiments is applied in a temperature control module to position a reagent tube when the reagent tube enters a certain area. Of course, in actual application, the adaptive compression assembly can also be applied to other objects other than reagent tubes.

[0069] In some embodiments, in combination with the description of Figure 1 , Figure 3 The adaptive compression assembly 100 includes a mounting seat 110, a movable floating compression member 120 arranged in the mounting seat 110, and an elastic member 130 arranged between the mounting seat 110 and the floating compression member 120. The floating compression member 120 is used to compress the reagent tube A at a target position. The floating compression member 120 is movably arranged in the mounting seat 110. The floating compression member 120 is adapted to compress or release the reagent tube A by moving relative to the mounting seat 110. The elastic member 130 is used to adaptively provide an elastic force to drive the floating compression member 120 to compress the reagent tube A.

[0070] The target position here refers to a position on the opposite side of the floating compression member 120. A compression space is formed between the floating compression member 120 and the target position. When the reagent tube A enters a certain temperature zone or a certain area, the reagent tube A is actively or passively squeezed into the compression space. The floating compression member 120 is adapted to move in a direction away from the target position according to the current squeezing degree of the reagent tube A into the compression space, so that the width of the compression space is adaptively changed. The elastic member 130 adaptively provides an elastic force, so that the floating compression member 120 is in force balance under the action of the elastic force and the squeezing force of the reagent tube A. The floating compression member 120 can always reliably compress the reagent in the compression space with variable width, tightly against the target position on the opposite side.

[0071] The reagent tube A in the above and below embodiments is taken as an example of the reagent tube shown in Figure 5 The reagent tube has a flat part A1 that fits into the compression space.

[0072] This adaptive clamping assembly ensures that the reagent tube remains firmly pressed against the target position on the opposite side throughout its journey from entering to leaving a specific area. Its adaptive capability prevents jamming or jamming, and the process causes minimal impact on the reagent tube, facilitating its smooth passage through the designated area. In practical applications, when the reagent tube traverses a temperature zone, a heat-conducting element can be placed at the target position on the opposite side or at the point of contact between the reagent tube A and the floating clamping assembly 120. This allows for rapid temperature transfer to the reagent tube A, achieving rapid temperature control of the reagent.

[0073] In some embodiments, see reference Figures 1 to 4 The floating clamping member 120 is provided with a clamping surface 120a for contacting the reagent tube A. When the reagent tube A is squeezed into the target position between the clamping surface 120a, the floating clamping member 120 moves adaptively under the action of the elastic member 130.

[0074] For ease of understanding, see [link to relevant documentation]. Figure 2 When reagent tube A is squeezed into the target position and the pressing space (such as X, Y or Z) of the pressing surface 120a, the floating pressing component 120 still moves adaptively under the action of the elastic component 130, which is conducive to keeping the pressing surface 120a in contact with the surface of the flat part A1 of reagent tube A, so that the reagent tube A is more reliably pressed when it enters the corresponding pressing space. If the pressing surface 120a is set on the heat-conducting element, the heat conduction efficiency can also be improved.

[0075] In some embodiments, see Figure 3 The mounting base 110 is provided with a guide groove 110c, the length direction of the guide groove 110c is along the pressing direction, and the floating pressing assembly 120 is provided with a guide part 140a, which is movably disposed in the guide groove 110c, so that the entire floating pressing assembly 120 can move relative to the mounting base 110. The pressing direction here is the direction away from or close to the target position. Figure 3 In this embodiment, the guide portion 140a corresponds to the two ends of the pin 140. In other embodiments (not shown), the floating clamping assembly is provided with a guide groove, the length direction of which is along the clamping direction, and the mounting base is provided with a guide portion, which is movably disposed within the guide groove.

[0076] In some embodiments, see Figure 3 The guide part 140a is a part of the pin 140. The floating clamping member 120 can rotate around the axis of the pin 140. Each elastic member 130 is distributed on both sides of the pin 140. Figure 3 In the middle, the elastic element 130 is a compression spring, and there are two elastic elements 130. One elastic element 130 is set on each side of the pin 140.

[0077] When the reagent tube A is extruded into the pressing space (such as X or Y or Z) between the target position and the pressing surface 120a, the floating pressing member 120 still moves adaptively under the action of the elastic member 130, and the floating pressing member 120 can rotate adaptively around the pin shaft 140 when the reagent tube A enters the pressing space (such as X or Y or Z).

[0078] If the floating pressing member 120 cannot rotate adaptively around the pin shaft, when the reagent tube A cannot enter the corresponding pressing space at a predetermined angle, that is, the surface of the flat portion A1 forms a smaller included angle with the pressing surface 120a, it will cause the pressing surface 120a and the flat portion of the reagent tube A to only have line contact, and cannot fully contact the surface of the flat portion A1 of the reagent tube A, which will cause low heat conduction efficiency when heat transfer is needed by the pressing surface 120a, and will affect the temperature control effect and detection efficiency. The structure that the floating pressing member 120 rotates adaptively around the pin shaft 140 makes the pressing surface 120a reliably close to the surface of the flat portion A1 of the reagent tube A, reliably realizes surface contact, and more stably and reliably presses the reagent tube A. If the pressing surface 120a is arranged on the heat conducting element, it is more conducive to heat transfer from other heat driven elements to the reagent tube A, and is more conducive to temperature rise or drop and improves detection efficiency.

[0079] In some embodiments, in combination with Figure 3 , Figure 5 , the reagent tube A has a flat portion A1 extruded into the target position and the pressing surface 120a, the surface of the flat portion A1 is a first inclined surface, the pressing surface 120a is a second inclined surface for being close to the first inclined surface, and the pressing surface 120a can fully contact the flat portion A1 of the reagent tube A when the flat portion A1 is extruded into the pressing surface 120a and the target position, which is reliable in pressing. If the pressing surface 120a is arranged on the heat conducting element, it is more conducive to heat transfer from other heat driven elements to the reagent tube A, and is more conducive to temperature rise or drop and improves detection efficiency.

[0080] In some embodiments, in combination with 3, the floating pressing member 120 is provided with a chamfer surface 120c for guiding the extrusion of the pressed object into the pressing surface 120a and the target position, and the two sides of the chamfer surface 120c are provided with transition circular arcs, and one side of the transition circular arcs is between the chamfer surface 120c and the pressing surface 120a. Here, the chamfer surface 120c is arranged, and transition circular arcs are arranged on both sides of the chamfer surface 120c, so that the reagent tube A can be more smoothly and reliably extruded into the pressing surface 120a and the target position.

[0081] In some embodiments, in combination with Figure 3The floating pressing assembly 120 comprises a first heat-conducting member 222 and a first heat-insulating member 224, and the pressing surface 120a is formed on the first heat-conducting member 222, and the first heat-insulating member 224 separates the first heat-conducting member 222 and the mounting base 110.

[0082] In some embodiments, the first heat-insulating member 224 comprises a heat-insulating plate 224a and a protrusion 224b provided on the heat-insulating plate 224a, and a pin hole is formed in the protrusion 224b; the mounting base 110 comprises a base plate 110a and a first lug 110b and a second lug provided on the base plate, and a waist-shaped hole serving as a guide groove 110c is formed in each of the first lug 110b and the second lug, wherein a spacing space 101 is formed between the heat-insulating plate 224a and the base plate, the first lug 110b, the second lug and the protrusion 224b are all located in the spacing space 101, the first lug 110b and the second lug are symmetrically distributed on two sides of the protrusion 224b, and the pin shaft 140 is arranged in the pin hole in a penetrating manner, and the two ends of the pin shaft 140 correspondingly penetrate the waist-shaped holes in the first lug 110b and the second lug. At this time, the pin shaft 140 is also located in the spacing space 101, so that the structure of the whole adaptive pressing unit is compact.

[0083] The following embodiments will list several application modes of the adaptive pressing assembly in the temperature control module, and in the following embodiments, the adaptive pressing assembly is selected from any one of the adaptive pressing assemblies 100 according to actual needs.

[0084] The application provides a floating pressing constant-temperature unit, and the adaptive pressing assembly 100 is applied to the floating pressing constant-temperature unit, and the adaptive pressing assembly 100 is combined with the floating pressing constant-temperature unit. Figure 1 、 Figure 2 、 Figure 3 The floating pressing constant-temperature unit comprises two groups of adaptive pressing assemblies 100, and the floating pressing assembly 120 of each of the two groups of adaptive units comprises a heating element 221; the heating element 221 is used for heating the first heat-conducting member 222, so that the temperature of the first heat-conducting member 222 is kept at a first target temperature, and the pressing surfaces 120a of the two groups of adaptive pressing assemblies 100 are all formed into heat-conducting pressing surfaces; the pressing surfaces 120a of the two groups of adaptive pressing assemblies 100 are opposite to each other and are separated from each other, so that an adaptive pressing space X for the reagent pipe A to be squeezed into or through is formed between the two pressing surfaces 120a.

[0085] The floating compression constant temperature unit can heat the reagent tube, the reagent tube A can be smoothly squeezed into the compression space X between the two compression surfaces 120a of the two adaptive compression assemblies 100, no matter where the reagent tube A is located in the compression space, the floating compression assembly 120 on both sides can realize reliable positioning of the reagent tube through adaptive floating, the reagent tube A can be in full contact with the heat-conducting compression surface at all times, so that the reagent tube A can exchange heat with the first heat-conducting member 222, and the heat-conducting compression surfaces on both sides of the reagent tube A heat the reagent tube A at the same time, heat exchange is faster, which is beneficial to make the temperature of the reagent tube A and the reagent inside the reagent tube A reach the corresponding first target temperature quickly.

[0086] Figure 3 In some embodiments, the heating element 221 is arranged between the first heat insulation member 224 and the first heat-conducting member 222 and is in full contact with the first heat-conducting member 222. In the figure, the lead of the part with the reference number 222 points to the lead connected to the heating element, but actually intends to refer to the heating element itself between the first heat insulation member 224 and the first heat-conducting member 222. In actual implementation, the heating element 221 can also be directly installed inside the first heat-conducting member 222 without being in contact with the first heat insulation member 224. In actual implementation, the heating element 221 can adopt a heating rod, a heating tube or the like. For the convenience of understanding, it needs to be explained that, Figure 3 In some embodiments, the reference number 221 of the heating element points to the lead connected to the heating element, but in the description of the present specification, the heating element 221 intends to refer to the heating element itself.

[0087] In some embodiments, the floating compression constant temperature unit comprises two adaptive compression assemblies 100, one of which is a first adaptive compression assembly and the other of which is a second adaptive compression assembly. The floating compression assembly 120 of the first adaptive compression assembly comprises a heating element 221, so that the compression surface 120a corresponding to the first adaptive compression assembly forms a heat-conducting compression surface. The heating element 221 is used to keep the temperature of the first heat-conducting member 222 at a first target temperature. The second adaptive compression assembly does not have the heating element 221. The compression surface of the first adaptive compression assembly and the compression surface of the second adaptive compression assembly face each other and are separated from each other, so that the adaptive compression space for the reagent tube A to be squeezed into or pass through is formed between the two compression surfaces.

[0088] No matter whether only one compression surface is a heat-conducting compression surface or both compression surfaces are heat-conducting compression surfaces, the target position of one side compression surface corresponds to the compression surface on the opposite side, which can realize reliable positioning of the reagent tube A and make the heat-conducting compression surface fully contact with the reagent tube A, so as to realize rapid and reliable heating of the reagent tube.

[0089] In some embodiments, referring to Figure 3The floating compression and pressing component 120 further comprises a first temperature acquisition element for acquiring the temperature of the first heat conducting component 222. The first temperature acquisition element can be a thermistor or the like.

[0090] When the reagent tube A is extruded into the compression space of the floating compression and constant temperature unit, heat exchange occurs between the first heat conducting component 222 and the reagent tube A. The resistance value of the thermistor changes according to the temperature of the first heat conducting component 222, so that the detection voltage or current of the corresponding detection circuit changes and is fed back to the controller or processor, etc. The controller or processor controls whether the heating element 221 heats the first heat conducting component 222 according to whether the acquired temperature value remains at the first target temperature, so that the temperature of the first heat conducting component 222 is always kept at the first target temperature.

[0091] Figure 3 In the embodiment, the first temperature acquisition element is between the first heat insulation component 224 and the first heat conducting component 222 and is installed in the groove 222a of the first heat conducting component 222. In actual implementation, the first temperature acquisition element can also be directly installed inside the first heat conducting component 222 without contacting the first heat insulation component 224.

[0092] The application further provides a floating compression and temperature changing unit, and the adaptive compression assembly 100 is applied to the floating compression and temperature changing unit, which is combined with reference to Figure 1 、 Figure 3 、 Figure 4 The floating compression and temperature changing unit comprises the adaptive compression assembly 100 and a temperature changing assembly 300. The temperature changing assembly 300 comprises a second heat conducting component 310 and a temperature changing element 320 for heating or cooling the second heat conducting component 310. The second heat conducting component 310 is provided with a heat conducting and pressing surface 331 for directly contacting the reagent tube A. The adaptive compression assembly 100 is any one of the adaptive compression assemblies 100 without the first heat conducting component 222. The heat conducting and pressing surface 331 and the pressing surface 120a face each other, and the compression space Y for extruding or passing the reagent tube A is formed between the heat conducting and pressing surface 331 and the pressing surface 120a.

[0093] After the reagent tube A is extruded into the compression space Y, the reagent tube A contacts the heat conducting and pressing surface 331 and the pressing surface 120a, respectively. The temperature changing element 320 can be used for heating or cooling the reagent tube A, so that rapid temperature rising or falling is achieved. Especially when the temperature changing element 320 is used for cooling the reagent tube A, the temperature difference between the reagent tube A and the second heat conducting component 310 can be kept within a preset range through gradual temperature reduction, so that the phenomenon of water droplets formed by condensation of the reagent tube A wall due to sudden temperature drop is avoided, and the phenomenon of insufficient reaction due to the same is avoided, which is beneficial to improving reliability.

[0094] In some embodiments, the variable temperature element 320 is a semiconductor refrigeration sheet, the variable temperature assembly 300 further comprises a heat dissipation seat 330, a second heat insulation member 340, a second temperature acquisition element 360 for acquiring the temperature of the heat dissipation seat 330, and a third temperature acquisition element 370 for acquiring the temperature of the second heat conduction member 310; the second heat insulation member 340 is arranged on the heat dissipation seat 330 and separates the heat dissipation seat 330 and the second heat conduction member 310; the second heat insulation member 340 is provided with a hollow mounting area 341, and the semiconductor refrigeration sheet is arranged in the hollow mounting area 341 at one end of the cold end and the hot end of the semiconductor refrigeration sheet, wherein one end is in contact with the heat dissipation seat, and the other end is in contact with the second heat conduction member.

[0095] When it is necessary to rapidly cool the reagent tube, the hot end is in contact with the heat dissipation seat 330, and the cold end is in contact with the second heat conduction member 310. The ordinary cooling mode has low cooling efficiency and is easy to condense into water temperature. This semiconductor refrigeration sheet cooling mode can realize rapid cooling of the reagent tube A, and can avoid condensation by multiple preset cold end target temperatures.

[0096] After the reagent tube A is extruded into the pressing space Y, the low temperature of the cold end is conducted to the reagent tube A through the second heat conduction member 310 to realize cooling. In the actual cooling process, the temperature of the second heat conduction member 310 can be first preset to a first low temperature, and the temperatures of the heat dissipation seat 330 and the second heat conduction member 310 are acquired in real time through the second temperature acquisition element 360 and the third temperature acquisition element 370. When the temperature of the second heat conduction member 310 reaches the preset first low temperature, the temperature of the second heat conduction member 310 is preset to a target low temperature lower than the first low temperature. The temperature of the second heat conduction member 310 drops to the first low temperature, and the temperature of the reagent tube A also drops to the second low temperature. Of course, the temperature of the second heat conduction member 310 can not be changed during the whole process. In the actual implementation process, the second temperature acquisition element 360 and the third temperature acquisition element 370 can also use a thermistor.

[0097] When it is necessary to heat the reagent tube, the hot end is in contact with the second heat conduction member 310, and the cold end is in contact with the heat dissipation seat 330, so that the reagent tube can be rapidly heated.

[0098] In some embodiments, the heat dissipation seat 330 is provided with a fan 350, which is beneficial to accelerate the heat dissipation of the heat dissipation seat 330.

[0099] For the convenience of understanding, it needs to be explained that, Figure 4 In some embodiments, the second temperature acquisition element 360 and the third temperature acquisition element 370 refer to the wires connected with the corresponding temperature acquisition elements, but in the description of the present application, the second temperature acquisition element 360 refers to the second temperature acquisition element itself, and the third temperature acquisition element 370 refers to the third temperature acquisition element itself.

[0100] The application further provides a floating compression mixing unit, the adaptive compression assembly 100 is applied to the floating compression mixing unit, and reference is made to Figure 1 , Figure 3 The floating compression mixing unit comprises an ultrasonic assembly and the adaptive compression assembly 100, the ultrasonic assembly comprises a vibration head 420 and an ultrasonic vibration source 410 for providing vibration power of the vibration head 420, and the adaptive compression assembly 100 is any one of the adaptive compression assemblies 100; a compression space Z for extruding or passing of the reagent tube A is formed between a top end of the vibration head 420 and the compression surface 120a of the adaptive compression assembly 100.

[0101] When the reagent tube A enters the floating compression mixing unit, the reagent tube A is extruded into the compression space Z between the top end of the vibration head 420 and the compression surface 120a, the vibration source 410 drives the vibration head 420 to vibrate, the vibration head 420 drives the reagent tube A and the reagent in the reagent tube A to vibrate, and the mixing of the reagent in the reagent tube A is realized; during the vibration process, the position of the reagent tube A is floated due to the vibration, and the adaptive compression assembly 100 can always reliably compress the reagent tube A between the vibration head 420 and the compression surface 120a until the reagent tube A leaves the compression space Z.

[0102] The application further provides a temperature control module, in some embodiments, referring to Figure 1 , the temperature control module comprises a carrier 500, and the carrier 500 is provided with a floating compression constant-temperature unit, a floating compression variable-temperature unit or a floating compression mixing unit; in actual implementation, the carrier 500 can be provided with only one or two of the floating compression constant-temperature unit, the floating compression variable-temperature unit or the floating compression mixing unit according to requirements.

[0103] In some embodiments, referring to Figure 1 , Figure 2 During the temperature control process, the reagent tube A is stationary, the carrier 500 is movable relative to the reagent tube A, the carrier 500 is provided with a reagent tube variable-temperature channel 501 for passing of the reagent tube A, the compression space of each unit forms a section of the reagent tube variable-temperature channel 501, and the extension direction of the reagent tube variable-temperature channel 501 is consistent with the moving direction of the carrier 500, so that when the carrier 500 moves, the reagent tube A enters the reagent tube variable-temperature channel 501 and passes through the compression space of the corresponding unit along the path of the reagent tube variable-temperature channel one by one. Of course, in actual implementation, as long as the reagent tube A and the carrier 500 can relatively rotate or move to enable the reagent tube A to enter the reagent tube variable-temperature channel 501, whether the reagent tube A is stationary and the carrier 500 moves or rotates, or the carrier 500 is stationary and the reagent tube A moves along the reagent tube variable-temperature channel 501.

[0104] The temperature control module can arrange the units along the reagent tube variable temperature channel 501 according to requirements, so that each reagent tube A can realize temperature control of each stage as long as it passes through the reagent tube variable temperature channel 501.

[0105] In some embodiments, referring to Figure 1 , the temperature control module comprises a rack 700 and a driving mechanism for driving the carrier 500 to move relative to the rack 700, and the reagent tube A is arranged stationary relative to the rack 700 during temperature control. The driving mechanism comprises a lead screw 610, a lead screw nut 620, and a power element 630. The lead screw 610 is arranged on the rack 700, and the arrangement direction of each unit is consistent with the axial direction of the lead screw 610. The lead screw nut 620 is arranged on the carrier 500, and the lead screw 610 and the lead screw nut are connected in cooperation. The power element 630 is arranged on the rack 700, and the power element 630 is used to provide power for the rotation of the lead screw 610.

[0106] This method of moving the carrier 500 by using the lead screw 610 and the lead screw nut can more accurately control the position of the moving stroke of the carrier 500 when it moves, which is beneficial to ensure that the reagent tube A can reliably enter another target unit from one unit, and is beneficial to ensure the reliability of each stage.

[0107] In some embodiments, the power element 630 is connected with a driving wheel 640, and a driven wheel 650 is arranged on the lead screw 610. The driving wheel 640 and the driven wheel 650 are connected by a synchronous belt. The power element 630 can be a motor, and at this time, the power element 630 and the lead screw 610 are equivalent to a flexible connection, which is beneficial to improve the service life of the power element 630.

[0108] In some embodiments, referring to Figure 1 , Figure 2 , the carrier 500 is provided with a floating pressure constant temperature heating unit, a floating pressure tight variable temperature unit, and a floating pressure tight mixing unit. The self-adaptive pressure assembly 100 in the floating pressure tight constant temperature unit located on one side of the pressure tight gap is arranged on the heat dissipation seat 330 of the refrigeration assembly, and the vibration source 410 in the floating pressure tight mixing unit is arranged on the heat dissipation seat 330.

[0109] In some embodiments, referring to Figure 6The carrier 500 is provided with three floating compression constant-temperature units, one floating compression variable-temperature unit and one floating compression mixing unit. The three floating compression constant-temperature units are respectively a first floating compression constant-temperature unit, a second floating compression constant-temperature unit and a third floating compression constant-temperature unit. The floating compression mixing unit, the floating compression variable-temperature unit, the first floating compression constant-temperature unit, the second floating compression constant-temperature unit and the third floating compression constant-temperature unit are arranged along the reagent tube variable-temperature channel 501 in sequence. The first heat-conducting member 222 of each floating compression constant-temperature unit is a constant-temperature member maintained at a specific temperature.

[0110] The floating compression mixing unit is used for mixing the reagent in the reagent tube A.

[0111] The temperature of the second heat-conducting member of the floating compression variable-temperature unit is lower than the target low temperature in a preset time period. The floating compression variable-temperature unit is used for cooling the reagent to a temperature close to the target low temperature. Since the temperature of the second heat-conducting member is lower than the target temperature, the reagent can be cooled quickly. The variable-temperature characteristic of the second heat-conducting member can be used to adjust the temperature of the second heat-conducting member to a suitable temperature, or the temperature of the second heat-conducting member can be gradually reduced to avoid condensation during the rapid cooling process.

[0112] The first heat-conducting member of the first floating compression constant-temperature unit is maintained at the target low temperature.

[0113] The first heat-conducting member of the second floating compression constant-temperature unit is maintained at a temperature higher than the target high temperature.

[0114] The first heat-conducting member of the third floating compression constant-temperature unit is maintained at the target high temperature.

[0115] For a PCR reaction, the whole process includes two reaction processes of lysis and expansion. During lysis, the reagent tube A needs to be heated. During expansion, the reagent tube A needs to be heated and cooled in cycles.

[0116] During lysis, the reagent tube A is first quickly heated to a temperature close to the target high temperature in the compression space X2 of the second floating compression constant-temperature unit, and then is extruded into the compression space X3 of the third floating compression constant-temperature unit, so that the temperature is maintained at the target high temperature for related reactions.

[0117] When amplification is needed, reagent is first added into the reagent tube A, which needs to be at a suitable reaction temperature in the initial stage of the reaction to avoid losing activity, so the reagent tube A can be first placed in the compression space Y of the floating compression variable temperature unit to be warmed to a suitable reaction temperature, then enter the compression space Z of the floating compression mixing unit to be mixed, after the mixing is completed, return to the compression space Y of the floating compression variable temperature unit to be static, and then enter the process of circulating temperature rising and falling of the reagent tube A. When the temperature rises, the reagent tube A enters the compression space X2 of the second floating compression constant temperature unit and the compression space X3 of the third floating compression constant temperature unit in turn to realize rapid temperature rising. When the temperature falls, the reagent tube A first enters the compression space Y of the floating compression variable temperature unit, and then enters the compression space X1 of the first floating compression constant temperature unit to realize rapid temperature falling.

[0118] The temperature control module can quickly realize temperature rising and falling, improve detection efficiency, realize rapid detection, and avoid condensation phenomenon during temperature falling by using the variable temperature characteristics of the floating compression variable temperature unit, which is beneficial to full reaction and improves detection results.

[0119] Any person skilled in the art can modify or change the above embodiments without departing from the spirit and scope of the present application. Therefore, all equivalent modifications or changes completed by those skilled in the art without departing from the spirit and technical thought disclosed by the present application should be covered by the claims of the present application.

Claims

1. An adaptive clamping assembly, characterized in that, include: Mounting base; A floating clamping member is used to clamp a reagent tube at a target position. The floating clamping member is movably mounted on the mounting base and adaptively clamps or releases the reagent tube by moving relative to the mounting base. An elastic member is disposed between the mounting base and the floating clamping member, and adaptively provides an elastic force to drive the floating clamping member to clamp the reagent tube. The floating clamping member has a clamping surface for contacting the reagent tube. When the reagent tube is squeezed between the target position and the clamping surface, the floating clamping member adaptively moves under the action of the elastic member. A guide is provided on the mounting base. The floating clamping assembly has a guide groove whose length direction is along the pressing direction, and a guide portion is provided on the floating clamping assembly, the guide portion being movably disposed within the guide groove; or the floating clamping assembly has a guide groove whose length direction is along the pressing direction, and a guide portion is provided on the mounting base, the guide portion being movably disposed within the guide groove; the guide portion is a pin, and the floating clamping assembly can rotate around the axis of the pin, with each elastic element distributed on both sides of the pin; when the reagent tube is squeezed into the target position between the pressing surface, the floating clamping assembly adaptively rotates around the pin under the action of the elastic elements.

2. The adaptive clamping assembly according to claim 1, characterized in that: The reagent tube has a flat portion between the insertion target position and the pressing surface, the surface of the flat portion being a first inclined surface, and the pressing surface being a second inclined surface for contacting the first inclined surface.

3. The adaptive clamping assembly according to claim 2, characterized in that: The floating clamping assembly is provided with a chamfered surface for guiding the pressed item to be squeezed into the space between the clamping surface and the target area. The chamfered surface has transition arcs on both sides, and one of the transition arcs is located between the chamfered surface and the clamping surface.

4. The adaptive clamping assembly according to any one of claims 1-3, characterized in that: The floating clamping assembly includes: a first heat-conducting element, on which the clamping surface is formed; and a first heat-insulating element, separating the first heat-conducting element from the mounting base.

5. The adaptive clamping assembly according to claim 4, characterized in that: The first heat insulation component includes a heat insulation plate and a protrusion disposed on the heat insulation plate, wherein a pin hole is provided on the protrusion; the mounting base includes a base plate and a first lug and a second lug disposed on the base plate, wherein the first lug and the second lug are each provided with an oblong hole serving as the guide groove, wherein a space is formed between the heat insulation plate and the base plate, the first lug, the second lug and the protrusion are all located within the space, the first lug and the second lug are symmetrically distributed on both sides of the protrusion, and the pin shaft is disposed through the pin hole, wherein both ends of the pin shaft pass through the oblong holes on the first lug and the second lug respectively.

6. The adaptive clamping assembly according to claim 4, characterized in that: The floating clamping component further includes: a heating element for heating the first heat-conducting component, the heating element being in contact with the first heat-conducting component; a first temperature acquisition element for acquiring the temperature of the first heat-conducting component; wherein the first temperature acquisition element is directly disposed on the first heat-conducting component; or the first temperature acquisition element is disposed between the first heat insulation component and the first heat-conducting component, and is in direct contact with the first heat-conducting component.

7. A floating compression constant temperature unit, characterized in that: The device includes two sets of adaptive clamping components, each of which is the adaptive clamping component as described in claim 6. The clamping surfaces of the two sets of adaptive clamping components face each other and are spaced apart, forming an adaptive clamping space between the two clamping surfaces for the reagent tube to be squeezed in or pass through. The heating element is used to maintain the temperature of the first heat-conducting element at a first target temperature. Alternatively, the adaptive clamping component includes a first adaptive clamping component and a second adaptive clamping component. The first adaptive clamping component is the adaptive clamping component as described in claim 6, and the heating element is used to maintain the temperature of the first heat-conducting element at a first target temperature. The second adaptive clamping component is the adaptive clamping component as described in any one of claims 1-5, and the second adaptive clamping component does not have the heating element. The clamping surfaces of the first and second adaptive clamping components face each other and are spaced apart, forming an adaptive clamping space between the two clamping surfaces for the reagent tube to be squeezed in or pass through.

8. A floating compression temperature-changing unit, characterized in that, include: A variable temperature assembly, comprising a second heat-conducting element and a variable temperature element for heating or cooling the second heat-conducting element, wherein the second heat-conducting element is provided with a heat-conducting pressing surface for direct contact with a reagent tube; an adaptive pressing assembly, wherein the adaptive pressing assembly is the adaptive pressing assembly according to any one of claims 1-5; wherein the heat-conducting pressing surface and the pressing surface face each other, and a pressing space is formed between the heat-conducting pressing surface and the pressing surface for the reagent tube to be squeezed in or pass through.

9. The floating compression temperature-changing unit according to claim 8, characterized in that, The temperature-changing element is a thermoelectric cooler. The temperature-changing assembly further includes a heat sink, a second heat insulation component, a second temperature acquisition element for acquiring the temperature of the heat sink, and a third temperature acquisition element for acquiring the temperature of the second heat conductor. The second heat insulation component is disposed on the heat sink and separates the heat sink and the second heat conductor. The second heat insulation component has a hollow mounting area, and the thermoelectric cooler is disposed in the hollow mounting area. One end of the thermoelectric cooler is in contact with the heat sink, and the other end is in contact with the second heat conductor.

10. The floating compression temperature-changing unit according to claim 9, characterized in that: A fan is installed on the heat sink.

11. A floating compression mixing unit, characterized in that, include: An ultrasonic assembly, the ultrasonic assembly including a vibrating head and an ultrasonic vibration source for providing vibration power to the vibrating head; An adaptive clamping assembly, wherein the adaptive clamping assembly is any one of claims 1-6; wherein a clamping space is formed between the top end of the vibrating head and the clamping surface of the adaptive clamping assembly for the reagent tube to be squeezed in or pass through.

12. A temperature control module, characterized in that: The device includes a carrier, on which at least one of a floating compression constant temperature unit, a floating compression variable temperature unit, or a floating compression mixing unit is provided, wherein the floating compression constant temperature unit is the floating compression constant temperature unit of claim 7, the floating compression variable temperature unit is the floating compression variable temperature unit of any one of claims 8-10, and the floating compression mixing unit is the floating compression mixing unit of claim 11.

13. The temperature control module according to claim 12, characterized in that: The carrier and the reagent tube can move or rotate relative to each other. The carrier is provided with a reagent tube temperature-changing channel for the reagent tube to pass through. The compression space corresponding to each unit forms a section of the reagent tube temperature-changing channel.

14. The temperature control module according to claim 13, characterized in that, The device includes a frame and a drive mechanism for moving the carrier relative to the frame. During temperature control, the reagent tube is stationary relative to the frame. The drive mechanism includes: a lead screw mounted on the frame, with each unit arranged in the same axial direction as the lead screw; a lead screw nut mounted on the carrier, and the lead screw and the lead screw nut engaging; and a power element mounted on the frame, which provides power for rotating the lead screw. The power element is connected to a drive pulley, and the lead screw has a driven pulley, with the drive pulley and driven pulley connected by a synchronous belt.

15. The temperature control module according to claim 13, characterized in that: The carrier is equipped with three floating compression temperature control units, one floating compression temperature variation unit, and one floating compression mixing unit. The three floating compression temperature control units are designated as a first floating compression temperature control unit, a second floating compression temperature control unit, and a third floating compression temperature control unit. These units are arranged sequentially along the temperature variation channel of the reagent tube. The floating compression mixing unit is used to mix the reagent within the tube. The second heat-conducting element of the floating compression temperature variation unit is kept below the target low temperature for a preset period. The first heat-conducting element of the first floating compression temperature control unit is kept at the target low temperature. The first heat-conducting element of the second floating compression temperature control unit is kept at a temperature rise transition temperature higher than the target high temperature. The first heat-conducting element of the third floating compression temperature control unit is kept at the target high temperature.

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