A method for cuttage seedling raising of the leaves of Acanthus ebracteatus Vahl

Abstract

The present invention provides a method for cutting propagation of the leaves of Acanthus ebracteatus, which relates to the technical field of plant asexual reproduction. The present invention uses leaves for cutting propagation, avoiding damage to the mother tree body of Acanthus ebracteatus, and realizing rapid propagation and seedling raising of Acanthus ebracteatus while retaining the mother tree body. It has reference significance for the protection, breeding and population expansion of other rare and endangered plants.

Description

Technical Field

[0001] The present invention belongs to the technical field of plant asexual reproduction, and particularly relates to a method for cutting and seedling raising with the leaves of Acanthus ebracteatus. Background Art

[0002] Acanthus ebracteatus is a true mangrove plant, belonging to the genus Acanthus of the family Acanthaceae. It is naturally distributed in Hainan, Guangdong and Guangxi of China, and is relatively abundant in Qinglan Port, Wenchang City, Hainan Province. In Hainan folk, the root of Acanthus ebracteatus is pounded and boiled in water, which is a specific medicine for treating hepatitis B; the fruit is cool in nature and slightly bitter in taste, with the effect of disinfecting and detumescence; the leaf extract has the function of protecting nerves; and in traditional Thai medicine, Acanthus ebracteatus is widely used and made into various products such as capsules and tea.

[0003] Due to the continuous decrease in the population of Acanthus ebracteatus in China in the past 30 years, according to the standards of the International Union for Conservation of Nature (IUCN), Acanthus ebracteatus in China belongs to the endangered level (EN). In 2006, Hainan Province has included it in the List of Wild Plants under Key Provincial Protection in Hainan Province. The population distribution range of Acanthus ebracteatus in Guangxi is extremely small, the habitat is narrow, and it is easily disturbed by humans. The situation is severe, and it is urgent to carry out rescue protection and population restoration for it. The traditional reproduction of Acanthus ebracteatus is by sexual reproduction, that is, using seeds for sowing, seedling raising and transplanting, but there are problems such as difficult seed collection, the collected seeds are not mature enough and cannot be effectively preserved, and the germination rate is low, which seriously affects the reproduction and growth of Acanthus ebracteatus. The vegetative propagation of Acanthus ebracteatus is mainly carried out by cutting cuttings for seedling propagation, but there are also some problems, such as: high requirements for the material of cuttings, difficult to obtain, and thus large-scale seedling raising cannot be carried out; Acanthus ebracteatus is an endangered plant, with a small number. Cutting branches to make cuttings will cause great harm to the mother tree and may exacerbate its endangered degree. At present, there is no report on the research of using leaves for cutting and seedling raising of Acanthus ebracteatus. Summary of the Invention

[0004] The purpose of the present invention is to provide a method for cutting and seedling raising with the leaves of Acanthus ebracteatus. Using leaves as cuttings can avoid harm to the mother tree of Acanthus ebracteatus, and while retaining the mother tree body, rapid propagation and seedling raising of Acanthus ebracteatus are achieved.

[0005] The present invention provides a method for cutting and seedling raising with the leaves of Acanthus ebracteatus, including the following steps: picking complete mature leaves from March to June every year, disinfecting them, and soaking the base of the leaf stalk with a rooting agent to obtain cuttings;

[0006] Inserting the cuttings into the substrate, the cutting depth is 1 / 3 of the height of the leaf, and after cutting, watering and disinfecting are carried out; the substrate is a mixture of sugarcane soil and coconut coir;

[0007] After cutting, shade and control water, and disinfect once every 10 days;

[0008] After 20 days of hardening off, the seedlings are taken out of the nursery.

[0009] Preferably, the complete mature leaves are from 3 - 5-year-old mother trees that are strong and disease-free. Pick the mature, plump, dark green and complete leaves in the middle of the branches, and remove the leaves from the base of the petioles.

[0010] Preferably, disinfect the picked complete mature leaves, including soaking and disinfecting them with an aqueous solution of carbendazim;

[0011] The aqueous solution of carbendazim includes a 500-fold solution of 50% carbendazim.

[0012] Preferably, the rooting agent includes a GGR6 rooting agent solution with a concentration of 1000 mg / L.

[0013] Preferably, soak the base of the petiole 2 - 3 cm with the rooting agent for 30 minutes.

[0014] Preferably, disinfect the substrate comprehensively 2 days before cutting;

[0015] After the disinfection, fill the substrate into a 32-hole seedling-raising tray. Under the seedling-raising tray is a rectangular plastic frame for controlling the water level and soil humidity.

[0016] Preferably, after cutting, shade and control water, including from the 0th to the 30th day after cutting, control the shading effect to be 20 - 30% of natural light; from the 31st day and later, control the shading effect to be 60 - 80% of natural light.

[0017] Preferably, after cutting, shade and control water, including from the 0th to the 20th day after cutting, control the water level in the rectangular plastic frame to submerge to 1 / 3 of the seedling-raising tray, and spray water daily to keep the substrate in a semi-moist state; from the 21st to the 30th day, adjust the water level in the rectangular plastic frame to 1 / 5, and keep the soil in a slightly moist state.

[0018] Preferably, after the 31st day after cutting, spray urea nutrient solution according to the growth situation; the mass percentage concentration of the urea nutrient solution is 1 - 2%.

[0019] Preferably, 20 days before the seedlings are taken out of the nursery, adjust the water level in the rectangular plastic frame to 3 / 5, and the salinity is 10; 10 days before the seedlings are taken out of the nursery, adjust the salinity to 20; after the hardening off is completed, the seedlings are taken out of the nursery.

[0020] Beneficial effects: The present invention provides a method for cutting and seedling the leaves of Acanthus ebracteatus. Intact mature leaves are picked as cuttings from March to June every year for leaf cutting. The cutting and seedling method no longer requires trimming branches, and for the first time, realizes cutting and seedling using leaves, which can avoid damaging the mother trees of Acanthus ebracteatus. While preserving the mother tree itself, it realizes the rapid propagation and seedling of Acanthus ebracteatus, which is of great significance for the breeding and protection of the endangered mangrove Acanthus ebracteatus, and has reference significance for the protection, breeding and population expansion of other rare and endangered plants. Detailed implementation mode

[0021] The present invention provides a method for cutting and seedling the leaves of Acanthus ebracteatus, comprising the following steps: picking intact mature leaves from March to June every year, soaking the base of the petiole with a rooting agent after disinfection to obtain cuttings;

[0022] Insert the cuttings into the substrate, and the cutting depth is 1 / 3 of the height of the leaf. After cutting, irrigate and disinfect; the substrate is a mixture of sugarcane soil and coconut coir;

[0023] Shade and control water after cutting, and disinfect once every 10 days;

[0024] After 20 days of acclimatization, the seedlings are taken out of the nursery.

[0025] The leaf cutting and seedling method of the present invention is preferably carried out for seedling from March to June every year, that is, intact mature leaves can be picked from March to June every year. The intact mature leaves are preferably from 3- to 5-year-old mother trees that are strong and disease-free. Pick the mature, plump and dark green intact leaves in the middle of the branches, and pick the leaves from the base of the petiole to avoid damaging the leaves and petioles; after the leaves are picked, to avoid sun exposure and high-temperature water loss, put the leaves into a foam box and spray to keep them moist.

[0026] Before cutting the picked leaves, the present invention preferably also includes a pretreatment process, specifically including disinfection and soaking with a rooting agent. The present invention disinfects the picked intact mature leaves, preferably including soaking and disinfecting with an aqueous solution of carbendazim; the aqueous solution of carbendazim preferably includes a 500-fold solution of 50% carbendazim. When the present invention performs the disinfection and rooting agent treatment, multiple leaves can be operated simultaneously. For example, tie the picked leaves into bundles of about 30 pieces with a rubber band, soak and disinfect in a 500-fold solution of 50% carbendazim (1 g is diluted with 500 ml of water) for 3 minutes, take out and wash with clean water, and then soak with the rooting agent. The rooting agent of the present invention preferably includes a GGR6 rooting agent solution, and the concentration is preferably 1000 mg / L. The soaking of the present invention preferably includes soaking the base of the petiole 2-3 cm with the rooting agent for 30 minutes.

[0027] The cuttings of the present invention are preferably carried out in a matrix, which is a mixture of sugarcane soil and coconut bran, and the volume ratio of the sugarcane soil to the coconut bran soaked in water is preferably 3:1. In the embodiment, sugarcane soil (soil rich in organic matter on the surface of the sugarcane field) and coconut bran (soaked in a small amount of clean water) are selected and mixed evenly in a volume ratio of 3:1. The matrix is ​​fully disinfected with 500 times of 50% carbendazim solution 2 days before cutting; after sufficient stirring, it is filled into a 32-hole seedling tray (single hole diameter 6 cm, depth 11 cm), and a rectangular plastic frame is under the seedling tray for controlling the water level and soil moisture.

[0028] When the cutting is performed, the cutting depth is about 1 / 3 of the leaf. Before cutting, a hole (3 cm long and 1 cm wide) is punched at the cutting position to avoid damaging the leaf and affecting the cutting effect, and then the leaf base is compacted with fine soil. After the cutting is completed, water is fully irrigated once, and 500 times of 50% carbendazim solution is immediately used for spraying and disinfection.

[0029] The present invention performs shading management after the cutting, and the shading management preferably adopts a double-layer sunshade net to build a shade shed, specifically: from the 0th to the 30th day after cutting, the shading effect is controlled to be 20-30% of natural light; from the 31st day and thereafter, the shading effect is controlled to be 60-80% of natural light. The present invention also includes water control after the cutting, specifically: shading and water control after cutting, including from the 0th to the 20th day of cutting, controlling the water level in the rectangular plastic frame to submerge to 1 / 3 of the seedling tray, and spraying moisture appropriately according to the daily weather conditions to keep the soil in a semi-wet state to prevent the leaves from drying up and dying; from the 21st to the 30th day, the base of the leaves begins to take root, and in order to avoid the base from rotting due to excessive water, the water level in the rectangular plastic frame needs to be adjusted to 1 / 5 to keep the soil in a slightly moist state.

[0030] According to the present invention, after the 31st day after the cutting, the leaves have a large amount of roots, and the urea nutrient solution can be sprayed according to the growth situation; the mass percentage concentration of the urea nutrient solution is 1-2%.

[0031] The present invention preferably further comprises hardening of seedlings before the seedlings are planted, specifically comprising: 20 days before the seedlings are planted, adjusting the water level in the rectangular plastic frame to 3 / 5, with a salinity of 10; 10 days before the seedlings are planted, adjusting the salinity to 20; and planting the seedlings after hardening is completed.

[0032] In order to further illustrate the present invention, a leaf cutting seedling raising method of Acanthus parviflorus provided by the present invention is described in detail below in conjunction with embodiments, but they should not be construed as limiting the protection scope of the present invention.

[0033] Example 1

[0034] Seedling raising time

[0035] In early June 2022, leaves were collected on the southwest side of Jiangshan Peninsula, Fangchenggang City, Fangchenggang District.

[0036] Leaf collection and transportation

[0037] Select healthy, disease-free, and insect-free mother trees that are 3-5 years old, pick mature, plump, dark green leaves from the middle of the branches, and carefully remove the leaves from the base of the petiole to avoid damaging the leaves and petioles; after the leaves are picked, in order to avoid exposure to the sun and high temperature water loss, put the leaves in a foam box and spray them to keep them moist. Then transport the leaves back to the Shijiao Base of Guangxi Mangrove Research Center in Fangchenggang City, Guangxi for cutting and seedling cultivation.

[0038] Leaf cutting pretreatment

[0039] Use rubber bands to tie the picked leaves into bundles of about 30 pieces, soak them in 500 times diluted 50% carbendazim for 3 minutes, take them out and wash them with clean water, soak 2-3 cm from the base of the petiole in GGR6 rooting agent solution (1000 mg / L) for 30 minutes, and then carry out cuttings.

[0040] Cutting medium preparation

[0041] Use sugarcane soil and coconut bran in a volume ratio of 3:1 and mix them evenly. Two days before cutting, use 500 times diluted 50% carbendazim to thoroughly disinfect the substrate. After fully stirring, fill it into a 32-hole seedling tray (single hole diameter 6cm, depth 11cm). Under the seedling tray is a rectangular plastic frame to control the water level and soil humidity.

[0042] Cuttings

[0043] The cutting depth is about 1 / 3 of the leaf. Before cutting, make a hole (3cm long and 1cm wide) at the cutting position to avoid damaging the leaves and affecting the cutting effect, and then compact the base of the leaves with fine soil. After the cutting is completed, water it once and immediately spray it with 500 times 50% carbendazim solution for disinfection.

[0044] Shade and management

[0045] Build a shade shed using a double-layer sunshade net, and control the shading effect at 20-30% of natural light in the early stage. From the 0th to the 20th day after cutting, control the water level in the rectangular plastic frame to submerge 1 / 3 of the seedling tray, and at the same time, spray and moisturize appropriately according to the daily weather conditions to keep the soil in a semi-moist state and avoid the leaves from drying and dying. From the 21st to the 30th day, roots begin to grow at the base of the leaves. To avoid basal rot caused by excessive moisture, adjust the water level in the rectangular plastic frame to 1 / 5, keeping the soil slightly moist. After the 31st day, control the shading effect at 60% of natural light. Since there are more roots growing from the leaves, spray urea nutrient solution (concentration 1-2%), and the survival rate is 90% at this time. On the 50th day, the cuttings germinate and grow true leaves, and the germination rate is 100%. After cutting, spray and disinfect with 500-fold solution of 50% carbendazim every 10 days once.

[0046] Hardening off and outplanting

[0047] Twenty days before outplanting the seedlings, adjust the water level in the rectangular plastic frame to 3 / 5, and the salinity is 10; ten days before outplanting the seedlings, adjust the salinity to 20; the seedlings can be outplanted after the hardening off is completed. After 90 days of cultivation, the emergence rate is 86%, the seedling height is 7-12 cm, and the number of true leaves is 8-14.

[0048] Example 2

[0049] Seeding time

[0050] Collect leaves on the southwest side of Jiangshan Peninsula, Fangchenggang City, in the first ten days of June 2022.

[0051] Leaf collection and transportation

[0052] Select 3-5-year-old mother trees with strong growth and no diseases and pests, pick the mature, plump and dark green complete leaves in the middle of the branches, carefully pick the leaves from the base of the petiole to avoid damaging the leaves and petioles; after the leaves are picked, to avoid sun exposure and high-temperature water loss, put the leaves into a foam box and spray and moisturize. Then transport the leaves back to the Shijiao Base of Guangxi Mangrove Research Center in Fangchenggang City, Guangxi for cuttage and seedling raising.

[0053] Pre-treatment of leaves before cutting

[0054] Tie the picked leaves into bundles of about 30 pieces with a rubber band, soak them in 500-fold solution of 50% carbendazim for 3 minutes, take them out, wash them with clean water, and then soak the base of the petiole 2-3 cm in GGR6 rooting agent solution (500 mg / L) for 60 minutes before cutting.

[0055] Preparation of cutting substrate

[0056] Select sugarcane soil and coconut coir and mix them evenly at a volume ratio of 3:1. Two days before cutting, thoroughly disinfect the substrate with a 500-fold solution of 50% carbendazim. After thorough stirring, fill it into a 32-hole seedling tray (single-hole diameter 6 cm, depth 11 cm). Under the seedling tray is a rectangular plastic frame for controlling the water level and soil humidity.

[0057] Cutting

[0058] The cutting depth is about 1 / 3 of the leaf. Before cutting, make holes (3 cm long, 1 cm wide) at the cutting position to avoid damaging the leaves and affecting the cutting effect, and then compact the base of the leaves with fine soil. After cutting, water thoroughly once and immediately spray and disinfect with a 500-fold solution of 50% carbendazim.

[0059] Shading and management

[0060] Use a double-layer sunshade net to build a shading shed, and control the initial shading effect at 20-30% of natural light. From the 0th to the 20th day after cutting, control the water level in the rectangular plastic frame to submerge to 1 / 3 of the seedling tray, and at the same time, spray and moisturize appropriately according to the daily weather conditions to keep the soil in a semi-moist state and avoid the leaves from drying and dying. From the 21st to the 30th day, roots begin to grow at the base of the leaves. To avoid rotting of the base due to excessive moisture, adjust the water level in the rectangular plastic frame to 1 / 5, and keep the soil in a slightly moist state. After the 31st day, control the shading effect at 60% of natural light. There are more roots growing from the leaves, and spray urea nutrient solution (concentration 1-2%). At this time, the survival rate is 82%. On the 50th day, the cuttings germinate and grow true leaves, and the germination rate is 85%. After cutting, spray and disinfect with a 500-fold solution of 50% carbendazim once every 20 days.

[0061] Hardening off and outplanting

[0062] Twenty days before the seedlings are outplanted, adjust the water level in the rectangular plastic frame to 3 / 5, and the salinity is 10; ten days before the seedlings are outplanted, adjust the salinity to 20; they can be outplanted after the hardening off is completed. After 90 days of cultivation, the emergence rate is 65%, the seedling height is 6-12 cm, and the number of true leaves is 6-12.

[0063] Example 3

[0064] Seedling raising time

[0065] Collect leaves on the southwest side of Jiangshan Peninsula, Fangcheng District, Fangchenggang City in the first ten days of June 2022.

[0066] Leaf collection and transportation

[0067] Select healthy, disease-free, and insect-free mother trees that are 3-5 years old, pick mature, plump, dark green leaves from the middle of the branches, and carefully remove the leaves from the base of the petiole to avoid damaging the leaves and petioles; after the leaves are picked, in order to avoid exposure to the sun and high temperature water loss, put the leaves in a foam box and spray them to keep them moist. Then transport the leaves back to the Shijiao Base of Guangxi Mangrove Research Center in Fangchenggang City, Guangxi for cutting and seedling cultivation.

[0068] Leaf cutting pretreatment

[0069] Use rubber bands to tie the picked leaves into bundles of about 30 pieces, soak them in 500 times diluted 50% carbendazim for 3 minutes, take them out and wash them with clean water, soak 2-3 cm from the base of the petiole in GGR6 rooting agent solution (1000 mg / L) for 30 minutes, and then carry out cuttings.

[0070] Cutting medium preparation

[0071] Sugarcane soil was selected as the cutting medium. Two days before cutting, the medium was fully disinfected with 500 times diluted 50% carbendazim. After being fully stirred, it was filled into a 32-hole seedling tray (single hole diameter 6 cm, depth 11 cm). Under the seedling tray was a rectangular plastic frame for controlling the water level and soil humidity.

[0072] Cuttings

[0073] The cutting depth is about 1 / 3 of the leaf. Before cutting, make a hole (3cm long and 1cm wide) at the cutting position to avoid damaging the leaves and affecting the cutting effect, and then compact the base of the leaves with fine soil. After the cutting is completed, water it once and immediately spray it with 500 times 50% carbendazim solution for disinfection.

[0074] Shade and management

[0075] Use double-layer sunshade net to build shade shed, and the early shading effect is controlled at 20-30% of natural light. From the 0th to the 20th day of cutting, control the water level in the rectangular plastic frame to submerge to 1 / 3 of the seedling tray, and spray moisture appropriately according to the daily weather conditions to keep the soil in a semi-humid state to avoid the leaves from drying up and dying. From the 21st to the 30th day, the base of the leaves begins to take root. To avoid excessive moisture and rot at the base, adjust the water level in the rectangular plastic frame to 1 / 5 to keep the soil in a slightly moist state. After the 31st day, the shading effect is controlled at 60% of natural light, and the leaves have more roots. Spray urea nutrient solution (concentration is 1-2%), and the survival rate is 85% at this time. On the 50th day, the cuttings sprout and grow true leaves, and the budding rate is 93%. After cutting, spray and disinfect once every 30 days with 50% carbendazim 500 times solution.

[0076] Hardening and seedling planting

[0077] 20 days before the seedlings are planted, adjust the water level in the rectangular plastic frame to 3 / 5 and the salinity to 10; 10 days before the seedlings are planted, adjust the salinity to 20; the seedlings can be planted after hardening. After 90 days of cultivation, the emergence rate is 78%, the seedling height is 6-10cm, and the number of true leaves is 6-12.

[0078] Example 4

[0079] Seedling raising time

[0080] In early June 2022, leaves were collected on the southwest side of Jiangshan Peninsula, Fangchenggang City, Fangchenggang District.

[0081] Leaf collection and transportation

[0082] Select healthy, disease-free, and insect-free mother trees that are 3-5 years old, pick mature, plump, dark green leaves from the middle of the branches, and carefully remove the leaves from the base of the petiole to avoid damaging the leaves and petioles; after the leaves are picked, in order to avoid exposure to the sun and high temperature water loss, put the leaves in a foam box and spray them to keep them moist. Then transport the leaves back to the Shijiao Base of Guangxi Mangrove Research Center in Fangchenggang City, Guangxi for cutting and seedling cultivation.

[0083] Leaf cutting pretreatment

[0084] Use rubber bands to tie the picked leaves into bundles of about 30 pieces, soak them in 500 times diluted 50% carbendazim for 3 minutes, take them out and wash them with clean water, soak 2-3 cm from the base of the petiole in GGR6 rooting agent solution (500 mg / L) for 60 minutes, and then carry out cuttings.

[0085] Cutting medium preparation

[0086] Red soil was selected as the cutting medium. Two days before cutting, the medium was fully disinfected with 500 times diluted 50% carbendazim. After being fully stirred, it was filled into a 32-hole seedling tray (single hole diameter 6 cm, depth 11 cm). Under the seedling tray was a rectangular plastic frame for controlling the water level and soil humidity.

[0087] Cuttings

[0088] The cutting depth is about 1 / 3 of the leaf. Before cutting, make a hole (3cm long and 1cm wide) at the cutting position to avoid damaging the leaves and affecting the cutting effect, and then compact the base of the leaves with fine soil. After the cutting is completed, water it once and immediately spray it with 500 times 50% carbendazim solution for disinfection.

[0089] Shade and management

[0090] Build a shading shed using a double-layer sunshade net, and control the shading effect at 20-30% of natural light in the early stage. From the 0th to the 20th day after cutting, control the water level in the rectangular plastic frame to submerge 1 / 3 of the seedling tray, and at the same time, carry out appropriate spraying and moisturizing according to the daily weather conditions to keep the soil in a semi-moist state and avoid the leaves from drying and dying. From the 21st to the 30th day, roots begin to grow at the base of the leaves. To avoid basal rot caused by excessive moisture, adjust the water level in the rectangular plastic frame to 1 / 5, and keep the soil in a slightly moist state. After the 31st day, control the shading effect at 60% of natural light. Since there are more roots growing from the leaves, spray urea nutrient solution (concentration 1-2%). At this time, the survival rate is 60%. On the 60th day, true leaves grow from the cuttings and the budding rate is 65%. After cutting, spray and disinfect once every 10 days with a 500-fold solution of 50% carbendazim.

[0091] Hardening off and outplanting

[0092] Twenty days before outplanting the seedlings, adjust the water level in the rectangular plastic frame to 3 / 5, and the salinity is 10; ten days before outplanting the seedlings, adjust the salinity to 20; the seedlings can be outplanted after the hardening off is completed. After 90 days of cultivation, the emergence rate is 42%, the seedling height is 4-8 cm, and the number of true leaves is 4-10.

[0093] Although the above embodiments have described the present invention in detail, they are only a part of the embodiments of the present invention, not all of them. People can also obtain other embodiments based on these embodiments without creative efforts, and these embodiments all fall within the protection scope of the present invention.

Claims

1. A method for leaf cutting and seedling raising of Acanthus ebracteatus Vahl, characterized in that, it includes the following steps: Pick complete mature leaves from March to June every year, soak the base of the petiole with a GGR6 rooting agent solution after disinfection to obtain cuttings; the concentration of the GGR6 rooting agent solution is 1000 mg / L, soak the base of the petiole 2 - 3 cm with the GGR6 rooting agent solution for 30 min; Insert the cuttings into the substrate, the cutting depth is 1 / 3 of the leaf height, and irrigate and disinfect after cutting; the substrate is a mixture of sugarcane soil and coconut coir; Shade and control water after cutting, disinfect once every 10 days; shade and control water after cutting, including from the 0th to the 30th day after cutting, control the shading effect to be 20 - 30% of natural light; on the 31st day and later, control the shading effect to be 60 - 80% of natural light; Hardening off for 20 days and then out of the nursery; 20 days before the seedlings are out of the nursery, adjust the water level in the rectangular plastic frame to 3 / 5, and the salinity is 10; 10 days before the seedlings are out of the nursery, adjust the salinity to 20; then out of the nursery after hardening off.

2. The leaf cutting and seedling raising method according to claim 1, characterized in that, the complete mature leaves are from 3 - 5-year-old mother trees that are growing vigorously and free of pests and diseases, pick the mature, plump and dark green complete leaves in the middle of the branches, and pick the leaves from the base of the petiole.

3. The leaf cutting and seedling raising method according to claim 1 or 2, characterized in that, disinfect the picked complete mature leaves, including soaking and disinfecting with an aqueous solution of carbendazim; the aqueous solution of carbendazim includes a 500-fold solution of 50% carbendazim.

4. The leaf cutting and seedling raising method according to claim 1, characterized in that, the substrate is comprehensively disinfected 2 days before cutting; after the disinfection, fill the substrate into a 32-hole seedling-raising tray, and there is a rectangular plastic frame under the seedling-raising tray for controlling the water level and soil humidity.

5. The leaf cutting and seedling raising method according to claim 1 or 4, characterized in that, shade and control water after cutting, including from the 0th to the 20th day after cutting, control the water level in the rectangular plastic frame to submerge 1 / 3 of the seedling-raising tray, and spray and moisturize every day to make the substrate in a semi-moist state; from the 21st to the 30th day, adjust the water level in the rectangular plastic frame to 1 / 5 to make the soil in a slightly moist state.

6. The leaf cutting and seedling raising method according to claim 1, characterized in that, after the 31st day after cutting, spray urea nutrient solution according to the growth situation; the mass percentage concentration of the urea nutrient solution is 1 - 2%.