A method and system for producing sodium hyaluronate

Abstract

The present application relates to the technical field of sodium hyaluronate production processing, comprising a feed mechanism, a reaction tank group, a separation unit and a purification unit, the feed mechanism is installed in the upstream of the reaction tank group and is used for supplementing raw materials to the inside of the reaction tank group, the separation unit is connected in the downstream of the reaction tank group, the separation unit is used for fully decomposing and processing the solution entering the inside, and the purification unit is connected through a filter delivery pipe in the downstream of the separation unit. The system adopts multiple reaction tanks, which can effectively control the reaction conditions of each reaction tank according to the needs, so as to obtain different reaction effect sodium hyaluronate crude product mixed solution, independent operation between each reaction tank can be effectively used for comparison and reference when producing a small sample, and the reaction efficiency and yield of the whole reaction process can also be improved according to the same reaction condition.

Description

Technical Field

[0001] This invention relates to the field of sodium hyaluronate production and processing technology, and in particular to a method and system for producing sodium hyaluronate. Background Technology

[0002] Sodium hyaluronate is a high-molecular-weight hydrophilic mucopolysaccharide with a cyclic structure that provides approximately 1000 times the binding capacity to water molecules. It also has a short half-life, is easily cleaved by endogenous creatine kinases, and exhibits no significant toxicity or inflammatory reactions. The production of sodium hyaluronate mainly falls into two categories: extraction using animal tissues and bacterial fermentation.

[0003] Among them, animal tissue extraction method is to extract from animal tissues and organs (such as rooster comb, bovine vitreous body, umbilical cord). The main extraction process steps include animal tissue, homogenization, extraction liquid, precipitation, solution, crude product, and refined product.

[0004] For example, patent document CN200810200148.4 discloses a method for purifying and optimizing sodium hyaluronate, the main steps of which include: 1) redissolving sodium hyaluronate at 80℃; 2) adding an appropriate amount of sodium salt; 3) passing the solution containing sodium hyaluronate through a positively charged nylon-66 polyamide resin membrane filter at 80℃; 4) adding sodium salt and water-soluble organic solvent to the filtrate; 5) precipitating the precipitate; 6) repeating the precipitation twice; 7) vacuum drying to obtain medical-grade sodium hyaluronate powder; wherein the sodium hyaluronate solution contains 0.001-1% (W / V) sodium hyaluronate; and hyaluronic acid produced by bacterial fermentation or tissue extraction is selected.

[0005] In addition, for example, patent document with patent application number CN201921279337.5 discloses a candle filter for extracting sodium hyaluronate. Its main structure includes a filter body, a top cover movably connected to the top of the filter body, a first connector fixedly connected to the bottom of the top cover, ...; a raw liquid inlet pipe is fixedly connected to the left side of the bottom of the filter body, a raw liquid outlet pipe is fixedly installed on the right side of the bottom of the filter body, and a residue outlet is opened at the bottom of the filter body.

[0006] As can be seen from the two patents in the prior art, in the process of producing sodium hyaluronate using the extraction method, due to the low content of the components, it is necessary to extract and filter the materials to the maximum extent in each process step in order to improve the quantity and purity of the product. However, the current purification process is difficult to effectively guarantee the purification effect and efficiency of sodium hyaluronate in the production of sodium hyaluronate, which makes the overall purification and production process of sodium hyaluronate relatively poor.

[0007] Therefore, this invention proposes a method that can effectively improve the efficiency and purification effect of the entire purification process for extracting sodium hyaluronate, thereby better solving the problems existing in the prior art. Summary of the Invention

[0008] To solve one of the aforementioned technical problems, the present invention provides a sodium hyaluronate production system, comprising a feed mechanism, a reaction tank assembly, a separation unit, and a purification unit. The feed mechanism is installed upstream of the reaction tank assembly and is used to replenish raw materials into the reaction tank assembly. The separation unit is connected downstream of the reaction tank assembly and is used to fully decompose the solution entering it. The purification unit is connected downstream of the separation unit via a post-filtration conveying pipe and is used to dehydrate and purify the sodium hyaluronate solution from upstream to obtain pure sodium hyaluronate.

[0009] In any of the above embodiments, it is preferred that the reaction tank group includes a plurality of reaction tanks arranged at intervals, the top inlet end of each reaction tank is respectively connected to the corresponding outlet end of the crushed material feeding mechanism, a thermometer, a pressure gauge and a feeding port with a sealing cap are respectively installed on the top of each reaction tank, and a primary material discharge branch pipe is respectively connected to the bottom outlet of each reaction tank, the end of each primary material discharge branch pipe is respectively connected in parallel to the main discharge pipeline, and the end of the main discharge pipeline is connected to the feed end of the separation unit;

[0010] Each of the aforementioned reaction vessels is equipped with an electric heating element and a temperature control unit; each of the aforementioned initial material discharge branch pipes is equipped with a discharge control solenoid valve, and a discharge power pump is installed on the aforementioned main discharge pipeline.

[0011] In any of the above embodiments, it is preferred that the crushing feeding mechanism includes a fixedly arranged feeding bin, a feeding stirring motor is installed on the top of the feeding bin, the motor shaft of the feeding stirring motor extends movably into the inside of the feeding bin and a feeding crushing cutter is fixedly connected to the end of the motor shaft of the feeding stirring motor, a plurality of feeding funnels communicating with the inside of the feeding bin are arranged on the top of the feeding bin, and a material conveying guide is connected to the bottom of the feeding bin.

[0012] In any of the above embodiments, it is preferred that the material conveying device includes a horizontal fine screw conveyor, the top inlet of the horizontal fine screw conveyor is connected to the bottom of the feed hopper, and a plurality of discharge risers with control valves are arranged sequentially at intervals along the length direction at the bottom of the shell of the horizontal fine screw conveyor. A bulk material discharge device is connected to the bottom of each discharge riser, and the end of each bulk material discharge device is connected to the feed inlet of the corresponding reaction tank on the reaction tank group.

[0013] In any of the above embodiments, it is preferred that the bulk material discharge device includes a conical platform fixedly installed at the bottom of the discharge riser. A plurality of material drop channels for material to fall are provided between the conical platform and the cavity of the discharge riser. A material dropping and fine crushing motor is installed on the top of the conical platform. A fine mixing and crushing blade is fixedly installed on the motor shaft of the material dropping and fine crushing motor. The fine mixing and crushing blade is used to further crush the material that has been initially agitated and falls from the corresponding discharge riser and falls down into the corresponding reaction tank along the material drop channels.

[0014] In any of the above embodiments, it is preferred that a plurality of blowing holes are provided on the surface of each of the conical platforms, and the bottom of the conical platform is connected to an external pulse pump body through a pneumatic pipeline. The pulse pump body blows upward pulse airflow to disperse the falling fragments and scatter them into the corresponding reaction tank, so as to ensure sufficient dispersion of the entering material and uniform distribution of the reactants after entering the reaction tank.

[0015] In any of the above embodiments, preferably, the separation unit includes a separation tank, a pressure separation unit is installed in the upper part of the separation chamber of the separation tank, a multi-stage filter assembly is installed in the separation tank below the pressure separation unit, the multi-stage filter assembly is used to filter and remove impurities from the crude sodium hyaluronate solution remaining in the separation tank after separation, and an oil storage unit is installed at the top of the separation tank, the oil storage unit is used to discharge the oil that is above the separation tank after settling to achieve separation from the lower layer of crude sodium hyaluronate solution.

[0016] In any of the above embodiments, preferably, the pressure separation unit includes a pressurizing electric cylinder fixedly installed at the top center of the separation tank. The piston rod of the pressurizing electric cylinder extends movably into the separation chamber and is fixedly connected to the top of a pressure piston. A feed channel and an oil discharge channel are respectively provided on both sides of the pressure piston. A feed riser and an oil discharge riser are respectively sealed and fixedly connected to the top of the feed channel and the oil discharge channel. The tops of both the feed riser and the oil discharge riser extend movably and sealed to the top of the separation tank. A feed bellows and an oil discharge bellows are respectively connected and installed at the top of each of the feed risers and the oil discharge risers. The inlet end of the feed bellows is connected to the discharge main pipeline, and the outlet end of the oil discharge bellows is connected to an oil pump. The outlet end of the oil pump is connected to the oil storage tank on the oil storage unit through an oil guide pipeline. A feed on / off valve and an oil discharge on / off valve are respectively installed on the feed risers and the oil discharge risers. When the pressure piston applies downward pressure, both the feed on / off valve and the oil discharge on / off valve are in the closed state.

[0017] In any of the above embodiments, it is preferred that the multi-stage filter assembly includes a large-mesh filter plate fixedly installed at the bottom of the separation chamber, a reduced-diameter riser connected to the bottom of the separation tank, and a medium-diameter filter and a small-diameter filter installed sequentially from upstream to downstream on the reduced-diameter riser, wherein the filtration accuracy of the small-diameter filter is greater than that of the medium-diameter filter and the large-mesh filter plate.

[0018] In any of the above embodiments, preferably, a coarse filter discharge port is installed on the reduced-diameter riser between the medium-diameter filter and the large-mesh filter plate; a medium filter discharge port is installed on the reduced-diameter riser between the medium-diameter filter and the small-diameter filter; a fine filter discharge port is installed on the reduced-diameter riser below the small-diameter filter; the ends of the coarse filter discharge port, the medium filter discharge port, and the fine filter discharge port are respectively connected to the post-filtration conveying pipe; a post-filtration conveying pump is installed on the post-filtration conveying pipe; and a filter channel solenoid opening valve is installed on the coarse filter discharge port, the medium filter discharge port, and the fine filter discharge port.

[0019] In any of the above embodiments, it is preferred that the purification unit includes a purification centrifuge connected to the end of the filtered conveying pipe, and the output end of the purification centrifuge is connected to downstream drying equipment.

[0020] This invention also provides a method for producing sodium hyaluronate using a sodium hyaluronate production system, comprising the following steps:

[0021] Preparation of materials:

[0022] Animal tissues used for purifying sodium hyaluronate are prepared, sterilized, and then cut into pieces for use.

[0023] Crushing and feeding:

[0024] After preparation, the blocky animal tissues are fed into the crushing feeding mechanism of the sodium hyaluronate production system in the correct amount, while adding an appropriate amount of sterile water and an appropriate amount of papaya decomposing enzyme.

[0025] Animal tissues entering the crushing and feeding mechanism will be crushed twice and then fully dispersed before entering the reaction tank group;

[0026] The animal tissue was fully reacted in each reaction vessel of the reaction vessel group, and a crude mixed solution containing sodium hyaluronate was initially obtained.

[0027] After the crude product mixed solution in each reaction vessel of the reaction vessel group has completed the reaction, each crude product mixed solution is sent into the separation unit for standing for 0.5h-1h. After standing, the upper and lower layers appear, with the upper layer being the oil solution and the lower layer being the degreased sodium hyaluronate crude product mixed solution.

[0028] Oil and liquid separation is accomplished by utilizing the pressure separation unit, multi-stage filter assembly, and oil storage tank within the separation unit.

[0029] The crude sodium hyaluronate mixture solution after oil removal and filtration is sent to the purification unit for purification treatment.

[0030] After purification in the purification unit or after dehydration of a mixed solution of pure sodium hyaluronate, pure sodium hyaluronate is obtained.

[0031] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0032] 1. This system uses multiple reaction vessels, which can effectively ensure that the reaction conditions of each reaction vessel are controlled as needed, so as to obtain crude sodium hyaluronate mixed solutions with different reaction effects. The independent operation of each reaction vessel can be effectively used for comparison and reference when producing small samples. Similarly, the reaction efficiency and yield of the entire reaction process can be improved by following the same reaction conditions.

[0033] 2. In order to ensure the reaction effect of the animal tissue raw materials that subsequently enter the reaction tank, the sodium hyaluronate production system of the present invention adopts a combination of feeding and stirring motor, feeding guide and discharging device to achieve initial crushing and feeding, fine crushing and dispersing. This process ensures that the material is fully crushed and that the subsequent feeding into the reaction tank is uniformly distributed. This ensures that the material is fully dispersed into the reaction tank and effectively prevents the lumps of crushed material from accumulating.

[0034] 3. The sodium hyaluronate production system of the present invention can effectively separate the reaction solution from the oil after the reaction, and can effectively complete the multi-stage filtration and impurity removal of the crude solution through pressure application. It can effectively ensure the filtration effect level as needed, and can achieve rapid purification by subsequent centrifugation, thereby effectively improving the overall preparation and production effect. Attached Figure Description

[0035] To more clearly illustrate the specific embodiments of the present invention or the technical solutions in the prior art, the accompanying drawings used in the description of the specific embodiments or the prior art will be briefly introduced below. In all the drawings, similar elements or components are generally identified by similar reference numerals. In the drawings, the elements or components are not necessarily drawn to scale.

[0036] Figure 1 This is a schematic diagram of the structure of the present invention.

[0037] Figure 2 This is a partially enlarged structural schematic diagram of the present invention.

[0038] Figure 3 for Figure 1A partially enlarged structural diagram of part A.

[0039] Figure 4 for Figure 1 A partially enlarged structural diagram of part B.

[0040] In the diagram: 1. Reaction vessel; 2. Thermometer; 3. Pressure gauge; 4. Feed inlet; 5. Initial material discharge branch pipe; 6. Main discharge pipe; 7. Discharge control solenoid valve; 8. Discharge power pump; 9. Feed hopper; 10. Feed stirring motor; 11. Feed crushing cutter; 12. Feed funnel; 13. Horizontal fine screw conveyor; 14. Discharge riser; 1401. Control valve; 15. Conical platform; 16. Material discharge channel; 17. Discharge fine crushing motor; 18. Fine crushing cutter; 19. Blowing hole; 20. Pneumatic pipeline; 21. Pulse pump body; 22. Separator; 23. Separation chamber; 24. Pressurization valve. 25. Cylinder; 26. Pressure piston; 27. Feed channel; 28. Oil discharge channel; 29. ​​Feed riser; 30. Oil discharge riser; 31. Feed bellows; 32. Oil discharge bellows; 33. Oil guide pipeline; 34. Oil storage tank; 35. Feed on / off valve; 36. Oil discharge on / off valve; 37. Large mesh filter plate; 38. Reduced diameter riser; 39. Medium diameter filter; 40. Small diameter filter; 41. Coarse filter discharge port; 42. Medium filter discharge port; 43. Fine filter discharge port; 44. Post-filter delivery pipe; 45. Post-filter delivery pump; 46. Filter channel solenoid opening valve; 47. Purification centrifuge. Detailed Implementation

[0041] The embodiments of the technical solution of the present invention will now be described in detail with reference to the accompanying drawings. The following embodiments are only used to more clearly illustrate the technical solution of the present invention, and are therefore merely examples and should not be used to limit the scope of protection of the present invention. The specific structure of the present invention is as follows: Figure 1-4 As shown in the image.

[0042] Example 1:

[0043] A sodium hyaluronate production system includes a feed mechanism, a reaction tank assembly, a separation unit, and a purification unit. The feed mechanism is installed upstream of the reaction tank assembly and is used to replenish the reaction tank assembly with raw materials. The separation unit is connected downstream of the reaction tank assembly and is used to fully decompose the solution entering it. The purification unit is connected downstream of the separation unit through a filter conveying pipe 44 and is used to dehydrate and purify the sodium hyaluronate solution from upstream to obtain pure sodium hyaluronate. The sodium hyaluronate production system utilizes a crushed material feeding mechanism to feed the material into the reaction tank group for a full reaction. After the reaction is completed, the oil-containing crude product mixture solution inside the reaction tank group is pumped into the corresponding separation unit. After settling inside the separation unit, the upper layer of oil accumulated inside the separation unit can be transported out, while the lower layer of oil-removed crude product mixture solution is sent into the purification unit for purification after multi-stage filtration. The purified sodium hyaluronate mixture solution is obtained as a pure product mixture solution, and after subsequent processing, pure sodium hyaluronate is obtained.

[0044] In any of the above embodiments, preferably, the reaction tank group includes several reaction tanks 1 spaced apart. The top inlet end of each reaction tank 1 is connected to the corresponding outlet end of the crushed material feeding mechanism. A thermometer 2, a pressure gauge 3, and a feed inlet 4 with a sealing cap are installed on the top of each reaction tank 1. A primary material discharge branch pipe 5 is connected to the bottom outlet of each reaction tank 1. The ends of each primary material discharge branch pipe 5 are connected in parallel to the main discharge pipeline 6. The end of the main discharge pipeline 6 is connected to the feed end of the separation unit. Each reaction tank 1 is equipped with an electric heating element and a temperature control unit. A discharge control solenoid valve 7 is installed on each primary material discharge branch pipe 5. A discharge power pump 8 is installed on the main discharge pipeline 6. The reaction tank group consists of multiple parallel reaction tanks. This centralized reaction method can effectively improve the overall production efficiency and production volume. During the production reaction in each reaction tank 1, the entire reaction process can be controlled by controlling the internal parameters such as heating, heat preservation, and pressurization. After the reaction is completed, the mixed solution can be sent to the downstream separation unit through the initial material discharge branch pipe 5 and the main discharge pipe 6. During the transportation process, only the solution inside one reaction tank 1 can be transported, or the solutions inside each reaction tank 1 can be transported outwards. The discharge power pump 8 can play the role of power transportation.

[0045] In any of the above embodiments, preferably, the separation unit includes a separation tank 22, a pressure separation unit is installed on the upper part of the separation chamber 23 of the separation tank 22, a multi-stage filter assembly is installed in the separation tank 22 below the pressure separation unit, the multi-stage filter assembly is used to filter and remove impurities from the crude sodium hyaluronate solution remaining in the separation tank 22 after separation, and an oil storage unit is installed on the top of the separation tank 22, the oil storage unit is used to discharge the oil that is above the separation tank 22 after settling to achieve separation from the lower layer of crude sodium hyaluronate solution. After the initial reaction, the mixture enters the separation unit. When the separation unit is working, it uses separation tank 22 to receive crude sodium hyaluronate solution from upstream. When the solution in separation tank 22 reaches a certain amount, the feeding is stopped and the mixture is allowed to stand for 0.5-1 hour. After the upper and lower layers separate, the upper layer of oil is separated using the oil storage unit. After the oil separation is completed, the pressure separation unit, in conjunction with the multi-stage filter assembly, is used to quickly remove slag from the oil-free mixed solution inside separation tank 22. The separated solution is then sent to the purification unit for further processing.

[0046] In any of the above embodiments, preferably, the pressure separation unit includes a pressure cylinder 24 fixedly installed at the top center of the separation tank 22. The piston rod of the pressure cylinder 24 extends movably into the separation chamber 23 and is fixedly connected to the top of a pressure piston 25. A feed channel 26 and an oil discharge channel 27 are respectively provided on both sides of the pressure piston 25. A feed riser 28 and an oil discharge riser 29 are respectively sealed and fixedly connected to the top of the feed channel 26 and the oil discharge channel 27. The tops of both the feed riser 28 and the oil discharge riser 29 extend movably and sealed to the top of the separation tank 22. The tops of the feed riser 28 and the oil discharge riser 29 are respectively connected to a feed bellows 30 and an oil discharge bellows 31. The inlet end of the feed bellows 30 is connected to the discharge main pipeline 6, and the outlet end of the oil discharge bellows 31 is connected to an oil pump 32. The outlet end of the oil pump 32 is connected to the oil storage tank 34 on the oil storage unit through an oil guide pipeline 33. A feed on / off valve 35 and an oil discharge on / off valve 36 are respectively installed on the feed riser 28 and the oil discharge riser 29. When the pressure piston 25 applies downward pressure, both the feed on / off valve 35 and the oil discharge on / off valve 36 are closed. The oil storage unit consists of an oil storage tank 34, an oil discharge pump 32, and an oil discharge bellows 31. When the pressure separation unit is working, it mainly closes the feed valve 35 and the drain valve 36, so that the pressure piston 25 is sealed when it applies pressure downward. At this time, when the pressure piston 25 applies pressure downward, it will drive the solution in the lower part of the separation tank 22 to flow downward. During the flow, the multi-stage filter assembly plays the role of pressure multi-stage filtration and filter. The specific number of filter stages can be controlled as needed.

[0047] In any of the above embodiments, preferably, the multi-stage filter assembly includes a large-mesh filter plate 37 fixedly installed at the bottom of the separation chamber 23, and a reduced-diameter riser 38 connected to the bottom of the separation tank 22. A medium-diameter filter 39 and a small-diameter filter 40 are sequentially installed on the reduced-diameter riser 38 from upstream to downstream at intervals. The filtration accuracy of the small-diameter filter 40 is greater than that of the medium-diameter filter 39 and the large-mesh filter plate 37. The multi-stage filter assembly here uses the large-mesh filter plate 37, the medium-diameter filter 39, and the small-diameter filter 40 to sequentially block slag materials of different particle sizes, effectively improving the filtration effect.

[0048] In any of the above embodiments, preferably, a coarse filter discharge port 41 is installed on the reduced-diameter riser 38 between the medium-diameter filter 39 and the large-mesh filter plate 37; a medium filter discharge port 42 is installed on the reduced-diameter riser 38 between the medium-diameter filter 39 and the small-diameter filter 40; and a fine filter discharge port 43 is installed on the reduced-diameter riser 38 below the small-diameter filter 40. The ends of the coarse filter discharge port 41, the medium filter discharge port 42, and the fine filter discharge port 43 are respectively connected to the post-filtration conveying pipe 44, and a post-filtration conveying pump 45 is installed on the post-filtration conveying pipe 44. A filter channel solenoid valve 46 is installed on each of the coarse filter discharge port 41, the medium filter discharge port 42, and the fine filter discharge port 43. The large-mesh filter plate 37, medium-diameter filter 39, and small-diameter filter 40 are arranged at intervals, and each stage of filtration is provided with a discharge port. This allows for the control of the discharge of solutions after different stages of filtration by controlling the opening of the discharge ports on different branches, effectively ensuring that the filtration effect is controllable.

[0049] Example 2:

[0050] A sodium hyaluronate production system includes a feed mechanism, a reaction tank assembly, a separation unit, and a purification unit. The feed mechanism is installed upstream of the reaction tank assembly and is used to replenish the reaction tank assembly with raw materials. The separation unit is connected downstream of the reaction tank assembly and is used to fully decompose the solution entering it. The purification unit is connected downstream of the separation unit through a filter conveying pipe 44 and is used to dehydrate and purify the sodium hyaluronate solution from upstream to obtain pure sodium hyaluronate.

[0051] In any of the above embodiments, it is preferred that the reaction tank group includes a plurality of reaction tanks 1 arranged at intervals, the top inlet end of each reaction tank 1 is respectively connected to the corresponding outlet end of the crushed material feeding mechanism, a thermometer 2, a pressure gauge 3 and a feeding port 4 with a sealing cap are respectively installed on the top of each reaction tank 1, and a primary material discharge branch pipe 5 is respectively connected to the bottom outlet of each reaction tank 1, the end of each primary material discharge branch pipe 5 is respectively connected in parallel to the discharge main pipeline 6, and the end of the discharge main pipeline 6 is connected to the feed end of the separation unit;

[0052] Each of the reaction vessels 1 is equipped with an electric heating element and a temperature control unit; each of the initial material discharge branch pipes 5 is equipped with a discharge control solenoid valve 7; and a discharge power pump 8 is installed on the main discharge pipeline 6.

[0053] The reaction tank group consists of multiple parallel reaction tanks. This centralized reaction method can effectively improve the overall production efficiency and production volume. During the production reaction in each reaction tank 1, the entire reaction process can be controlled by controlling the internal parameters such as heating, heat preservation, and pressurization. After the reaction is completed, the mixed solution can be sent to the downstream separation unit through the initial material discharge branch pipe 5 and the main discharge pipe 6. During the transportation process, only the solution inside one reaction tank 1 can be transported, or the solutions inside each reaction tank 1 can be transported outwards. The discharge power pump 8 can play the role of power transportation.

[0054] In any of the above embodiments, it is preferred that the crushing feeding mechanism includes a fixedly arranged feeding bin 9, a feeding stirring motor 10 is installed on the top of the feeding bin 9, the motor shaft of the feeding stirring motor 10 extends movably into the interior of the feeding bin 9 and a feeding crushing cutter 11 is fixedly connected to the end of the motor shaft of the feeding stirring motor 10, a plurality of feeding funnels 12 connected to the interior of the feeding bin 9 are provided on the top of the feeding bin 9, and a material conveying guide is connected to the bottom of the feeding bin 9.

[0055] The lumpy material is fed into the feed hopper 12, and the feed stirring motor 10 drives the feed crushing blade 11 to rotate and stir the material in the feed bin 9. This can achieve rapid primary crushing of the lumpy animal tissue raw material that enters the bin. The crushed material is then conveyed through the feed guide and sequentially transported to the inlet end of the corresponding reaction tank 1 under the action of the feed guide.

[0056] In any of the above embodiments, it is preferred that the material conveying device includes a horizontal fine screw conveyor 13, the top inlet of the horizontal fine screw conveyor 13 is connected to the bottom of the feed hopper 9, and a plurality of discharge risers 14 with control valves 1401 are arranged sequentially at intervals along the length direction at the bottom of the shell of the horizontal fine screw conveyor 13. A bulk material discharge device is connected to the bottom of each discharge riser 14, and the end of each bulk material discharge device is connected to the feed inlet of the corresponding position of the reaction tank 1 on the reaction tank group.

[0057] The material conveyor here adopts a horizontal fine screw conveyor 13. When conveying crushed materials, the operation of the horizontal fine screw conveyor 13 can drive the crushed materials inside it to pass through the corresponding discharge riser 14 in sequence. When the valve on the discharge riser 14 at the corresponding position is in the open state, the material can directly enter the bulk material discharger from the current discharge riser 14 and undergo a second fine crushing inside the bulk material discharger. When the crushed materials after the second fine crushing enter the reaction tank 1, a highly efficient and rapid reaction can be achieved, shortening the reaction time and cycle.

[0058] In any of the above embodiments, it is preferred that the bulk material discharger includes a conical platform 15 fixedly installed at the bottom of the discharge riser 14. A plurality of material drop channels 16 for material to fall are provided between the conical platform 15 and the cavity of the discharge riser 14. A material dropping and fine crushing motor 17 is installed on the top of the conical platform 15. A fine mixing and crushing cutter 18 is fixedly installed on the motor shaft of the material dropping and fine crushing motor 17. The fine mixing and crushing cutter 18 is used to further crush the material that has been initially agitated and falls from the corresponding discharge riser 14 and falls down along the material drop channels 16 into the corresponding reaction tank 1.

[0059] The initial crushed raw material entering the bulk material discharge device will be subjected to high-speed secondary crushing by the fine crushing blades 18 under the action of the fine crushing motor 17. The crushed raw material will fall into the reaction tank 1 for later use. The primary and secondary crushing can effectively ensure that the particle size accuracy of the material entering the reaction tank 1 meets the standard requirements.

[0060] In any of the above embodiments, it is preferred that a plurality of blowing holes 19 are provided on the surface of each of the conical platforms 15, and the bottom of the conical platform 15 is connected to an external pulse pump body 21 through a pneumatic pipeline 20. The pulse pump body 21 blows upward pulse airflow to disperse the falling fragments and scatter them into the corresponding reaction tank 1, so as to ensure sufficient dispersion of the entering material and uniform distribution of the reactants after entering the reaction tank 1.

[0061] The reaction raw materials with particle size accuracy meeting the standard requirements are fully dispersed by the high-pressure pulse airflow emitted by the pulse pump body 21 during the falling process through each blowing hole 19, thereby ensuring that the crushed raw materials entering the reaction tank 1 are fully dispersed.

[0062] In any of the above embodiments, preferably, the separation unit includes a separation tank 22, a pressure separation unit is installed on the upper part of the separation chamber 23 of the separation tank 22, a multi-stage filter assembly is installed in the separation tank 22 below the pressure separation unit, the multi-stage filter assembly is used to filter and remove impurities from the crude sodium hyaluronate solution remaining in the separation tank 22 after separation, and an oil storage unit is installed on the top of the separation tank 22, the oil storage unit is used to discharge the oil that is above the separation tank 22 after settling to achieve separation from the lower layer of crude sodium hyaluronate solution.

[0063] After the initial reaction, the mixture enters the separation unit. When the separation unit is working, it uses separation tank 22 to receive crude sodium hyaluronate solution from upstream. When the solution in separation tank 22 reaches a certain amount, the feeding is stopped and the mixture is allowed to stand for 0.5-1 hour. After the upper and lower layers separate, the upper layer of oil is separated using the oil storage unit. After the oil separation is completed, the pressure separation unit, in conjunction with the multi-stage filter assembly, is used to quickly remove slag from the oil-free mixed solution inside separation tank 22. The separated solution is then sent to the purification unit for further processing.

[0064] In any of the above embodiments, preferably, the pressure separation unit includes a pressure cylinder 24 fixedly installed at the top center of the separation tank 22. The piston rod of the pressure cylinder 24 extends movably into the separation chamber 23 and is fixedly connected to the top of a pressure piston 25. A feed channel 26 and an oil discharge channel 27 are respectively provided on both sides of the pressure piston 25. A feed riser 28 and an oil discharge riser 29 are respectively sealed and fixedly connected to the top of the feed channel 26 and the oil discharge channel 27. The tops of both the feed riser 28 and the oil discharge riser 29 extend movably and sealed to the top of the separation tank 22. The top of the feed riser 28 and the oil discharge riser 29 are respectively connected to a feed bellows 30 and an oil discharge bellows 31. The inlet end of the feed bellows 30 is connected to the discharge main pipeline 6, and the outlet end of the oil discharge bellows 31 is connected to an oil pump 32. The outlet end of the oil pump 32 is connected to the oil storage tank 34 on the oil storage unit through an oil guide pipeline 33. A feed on / off valve 35 and an oil discharge on / off valve 36 are respectively installed on the feed riser 28 and the oil discharge riser 29. When the pressure piston 25 applies downward pressure, both the feed on / off valve 35 and the oil discharge on / off valve 36 are in the closed state.

[0065] The oil storage unit consists of an oil storage tank 34, an oil discharge pump 32, and an oil discharge bellows 31.

[0066] When the pressure separation unit is working, it mainly closes the feed valve 35 and the drain valve 36, so that the pressure piston 25 is sealed when it applies pressure downward. At this time, when the pressure piston 25 applies pressure downward, it will drive the solution in the lower part of the separation tank 22 to flow downward. During the flow, the multi-stage filter assembly plays the role of pressure multi-stage filtration and filter. The specific number of filter stages can be controlled as needed.

[0067] In any of the above embodiments, it is preferred that the multi-stage filter assembly includes a large-mesh filter plate 37 fixedly installed at the bottom of the separation chamber 23, a reduced-diameter riser 38 connected to the bottom of the separation tank 22, and a medium-diameter filter 39 and a small-diameter filter 40 installed sequentially from upstream to downstream on the reduced-diameter riser 38, wherein the filtration accuracy of the small-diameter filter 40 is greater than that of the medium-diameter filter 39 and the large-mesh filter plate 37.

[0068] The multi-stage filter assembly uses a large-mesh filter plate 37, a medium-diameter filter 39, and a small-diameter filter 40 to block slag of different particle sizes in sequence, effectively improving the filtration effect.

[0069] In any of the above embodiments, preferably, a coarse filter discharge port 41 is installed on the reduced-diameter riser 38 between the medium-diameter filter 39 and the large-mesh filter plate 37; a medium filter discharge port 42 is installed on the reduced-diameter riser 38 between the medium-diameter filter 39 and the small-diameter filter 40; and a fine filter discharge port 43 is installed on the reduced-diameter riser 38 below the small-diameter filter 40. The ends of the coarse filter discharge port 41, the medium filter discharge port 42, and the fine filter discharge port 43 are respectively connected to the post-filtration conveying pipe 44, and a post-filtration conveying pump 45 is installed on the post-filtration conveying pipe 44. A filter channel solenoid valve 46 is installed on each of the coarse filter discharge port 41, the medium filter discharge port 42, and the fine filter discharge port 43.

[0070] The large-mesh filter plate 37, medium-diameter filter 39, and small-diameter filter 40 are arranged at intervals, and each stage of filtration is provided with a discharge port. This allows for the control of the discharge of solutions after different stages of filtration by controlling the opening of the discharge ports on different branches, effectively ensuring that the filtration effect is controllable.

[0071] In any of the above embodiments, it is preferred that the purification unit includes a purification centrifuge 47 connected to the end of the filtered conveying pipe 44, and the output end of the purification centrifuge 47 is connected to a downstream drying device.

[0072] The sodium hyaluronate production system utilizes a crushed material feeding mechanism to feed the material into the reaction tank group for a full reaction. After the reaction is completed, the oil-containing crude product mixture solution inside the reaction tank group is pumped into the corresponding separation unit. After settling inside the separation unit, the upper layer of oil accumulated inside the separation unit can be transported out, while the lower layer of oil-removed crude product mixture solution is sent into the purification unit for purification after multi-stage filtration. The purified sodium hyaluronate mixture solution is obtained as a pure product mixture solution, and after subsequent processing, pure sodium hyaluronate is obtained.

[0073] This invention also provides a method for producing sodium hyaluronate using a sodium hyaluronate production system, comprising the following steps:

[0074] Preparation of materials:

[0075] Animal tissues used for purifying sodium hyaluronate are prepared, sterilized, and then cut into pieces for use.

[0076] Crushing and feeding:

[0077] The prepared block animal tissues are fed into the crushing and feeding mechanism of the sodium hyaluronate production system in sequence according to the amount. At the same time, an appropriate amount of sterile water and an appropriate amount of papaya decomposing enzyme are added. The block material is fed in through the feeding funnel 12. The feeding stirring motor 10 drives the feeding crushing blade 11 to rotate and stir the material in the feeding bin 9. This can realize the rapid primary crushing of the block animal tissue raw material that enters it. The crushed material will be conveyed through the conveying guide and then conveyed to the inlet end of the corresponding reaction tank 1 in sequence under the action of the conveying guide.

[0078] Animal tissues entering the crushing and feeding mechanism will be crushed twice and then fully dispersed before entering the reaction tank group;

[0079] Animal tissues are fully reacted in each reaction tank 1 of the reaction tank group to initially obtain a crude mixed solution containing sodium hyaluronate. After the initial reaction, the mixed solution enters the separation unit. When the separation unit is working, the separation tank 22 receives the crude sodium hyaluronate solution from the upstream. When the solution in the separation tank 22 reaches a certain amount, the feeding is stopped and the solution is kept still for 0.5-1 hours. After the upper and lower layers are separated, the upper layer of oil is separated by the oil storage unit. After the oil separation is completed, the pressure separation unit is used in conjunction with the multi-stage filter assembly to quickly remove slag from the oil-free mixed solution inside the separation tank 22. The separated solution is sent to the purification unit for further processing.

[0080] After the crude product mixed solution in each reaction tank 1 of the reaction tank group has completed the reaction, each crude product mixed solution is sent into the separation unit for standing for 0.5h-1h. After standing, the upper and lower layers appear, with the upper layer being the oil solution and the lower layer being the degreased sodium hyaluronate crude product mixed solution.

[0081] Oil and liquid separation is accomplished by utilizing the pressure separation unit, multi-stage filter assembly, and oil storage tank 34 within the separation unit.

[0082] The crude sodium hyaluronate mixture solution after oil removal and filtration is sent to the purification unit for purification treatment.

[0083] After purification in the purification unit or after dehydration of a mixed solution of pure sodium hyaluronate, pure sodium hyaluronate is obtained.

[0084] The above embodiments are only used to illustrate the technical solutions of the present invention, and are not intended to limit it. Although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some or all of the technical features therein. Such modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the scope of the technical solutions of the embodiments of the present invention, and they should all be covered within the scope of the claims and specification of the present invention. For those skilled in the art, any alternative improvements or modifications made to the embodiments of the present invention fall within the protection scope of the present invention.

[0085] Any aspects of this invention not described in detail are well-known to those skilled in the art.

Claims

1. A sodium hyaluronate production system, characterized in that: The system includes a crushing feed mechanism, a reaction tank assembly, a separation unit, and a purification unit. The crushing feed mechanism is installed upstream of the reaction tank assembly and is used to replenish the raw materials inside the reaction tank assembly. The separation unit is connected downstream of the reaction tank assembly and is used to fully decompose the solution entering it. Downstream of the separation unit, a purification unit is connected through a filter conveying pipe. The purification unit is used to dehydrate and purify the sodium hyaluronate solution from upstream to obtain pure sodium hyaluronate. The crushing and feeding mechanism includes a fixedly installed feeding hopper. A feeding and stirring motor is installed on the top of the feeding hopper. The motor shaft of the feeding and stirring motor extends movably into the inside of the feeding hopper, and a feeding and crushing blade is fixedly connected to the end of the motor shaft. Several feeding funnels communicating with the inside of the feeding hopper are provided on the top of the feeding hopper. A material conveyor is connected to the bottom of the feeding hopper. The material conveyor includes a horizontal fine screw conveyor. The top inlet of the horizontal fine screw conveyor is connected to the bottom of the feeding hopper. Several discharge risers with control valves are arranged sequentially and at intervals along the length of the bottom of the casing of the horizontal fine screw conveyor. A bulk material discharge device is connected to the bottom of each discharge riser. The ends of the bulk material discharge device are respectively connected to the feed inlets of the corresponding reaction tanks on the reaction tank assembly; the bulk material discharge device includes a conical platform fixed to the bottom of the discharge riser, and several material dropping channels are provided between the conical platform and the cavity of the discharge riser. A material dropping and fine crushing motor is installed on the top of the conical platform, and a fine mixing and crushing blade is fixed on the motor shaft of the material dropping and fine crushing motor; several blowing holes are provided on the surface of each conical platform, and the bottom of the conical platform is connected to an external pulse pump body through a pneumatic pipeline. The pulse pump body blows upward pulse airflow to disperse the downward falling crushed material and scatter it into the corresponding reaction tank, ensuring that the reactants are evenly distributed after entering the reaction tank; The separation unit includes a separation tank, a pressure separation unit installed in the upper part of the separation chamber of the separation tank, and a multi-stage filter assembly installed in the separation tank below the pressure separation unit. The multi-stage filter assembly is used to filter and remove impurities from the crude sodium hyaluronate solution remaining in the separation tank after separation. An oil storage unit is installed at the top of the separation tank. The oil storage unit is used to discharge the oil that is above the separation tank after settling and to separate it from the lower layer of crude sodium hyaluronate solution.

2. The sodium hyaluronate production system according to claim 1, characterized in that: The reaction tank group includes several reaction tanks spaced apart. The top inlet of each reaction tank is connected to the corresponding outlet of the crushed material feeding mechanism. A thermometer, a pressure gauge, and a feed port with a sealing cap are installed on the top of each reaction tank. A primary material discharge branch pipe is connected to the bottom outlet of each reaction tank. The ends of each primary material discharge branch pipe are connected in parallel to the main discharge pipeline. The end of the main discharge pipeline is connected to the feed end of the separation unit. Each of the aforementioned reaction vessels is equipped with an electric heating element and a temperature control unit; each of the aforementioned initial material discharge branch pipes is equipped with a discharge control solenoid valve, and a discharge power pump is installed on the aforementioned main discharge pipeline.

3. The sodium hyaluronate production system according to claim 2, characterized in that: The pressure separation unit includes a pressure cylinder fixedly installed at the top center of the separation tank. The piston rod of the pressure cylinder extends movably into the separation chamber and is fixedly connected to the top of a pressure piston. A feed channel and an oil discharge channel are respectively provided on both sides of the pressure piston. A feed riser and an oil discharge riser are respectively sealed and fixedly connected to the top of the feed channel and the oil discharge channel. The tops of both the feed riser and the oil discharge riser extend movably and sealed to the top of the separation tank. A feed bellows and an oil discharge bellows are respectively connected to the top of the pipe and the oil discharge riser. The inlet end of the feed bellows is connected to the discharge main pipeline, and the outlet end of the oil discharge bellows is connected to an oil pump. The outlet end of the oil pump is connected to the oil storage tank on the oil storage unit through an oil guide pipeline. A feed on / off valve and an oil discharge on / off valve are respectively installed on the feed riser and the oil discharge riser. When the pressure piston applies downward pressure, both the feed on / off valve and the oil discharge on / off valve are in the closed state.

4. The sodium hyaluronate production system according to claim 3, characterized in that: The multi-stage filter assembly includes a large-mesh filter plate fixedly installed at the bottom of the separation chamber, a reduced-diameter riser connected to the bottom of the separation tank, and a medium-diameter filter and a small-diameter filter installed sequentially from upstream to downstream on the reduced-diameter riser. The filtration accuracy of the small-diameter filter is greater than that of the medium-diameter filter and the large-mesh filter plate.

5. The sodium hyaluronate production system according to claim 4, characterized in that: A coarse filter outlet is installed on the reduced-diameter riser between the medium-diameter filter and the large-mesh filter plate. A medium filter outlet is installed on the reduced-diameter riser between the medium-diameter filter and the small-diameter filter. A fine filter outlet is installed on the reduced-diameter riser below the small-diameter filter. The ends of the coarse filter outlet, the medium filter outlet, and the fine filter outlet are respectively connected to the post-filtration delivery pipe. A post-filtration delivery pump is installed on the post-filtration delivery pipe. A filter channel solenoid valve is installed on each of the coarse filter outlet, the medium filter outlet, and the fine filter outlet.

6. The sodium hyaluronate production system according to claim 5, characterized in that: The purification unit includes a purification centrifuge connected to the end of the filtered conveying pipe, and the output end of the purification centrifuge is connected to a downstream drying device.

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