Liquid transfer device for gene detection

Abstract

The present invention discloses a pipetting device for gene detection. The pipetting device for gene detection includes a pipetting platform and a pipetting device. A plurality of reagent containers are placed on the pipetting platform, and the reagent containers are used for placing liquids to be configured. The pipetting device is suspended above the pipetting platform and can move between the plurality of reagent containers. The pipetting device includes a mounting bracket, a single-channel injection pump, and a multi-channel injection pump. The single-channel injection pump and the multi-channel injection pump are both fixed on the mounting bracket. The mounting bracket moves between the plurality of reagent containers, and both the single-channel injection pump and the multi-channel injection pump are used for transferring the liquids to be configured between the plurality of reagent containers. The technical solution of the present invention can reduce the detection cost of gene sequencing.

Description

Technical Field

[0001] The present invention relates to the technical field of gene detection, and particularly relates to a pipetting device for gene detection. Background Art

[0002] With the continuous development of gene sequencing technology, gene sequencing has become an important means for clinical trial detection. Before gene sequencing, it is necessary to use a pipetting device to perform multiple pipetting, mixing and other processes on different types of liquids. Therefore, the pipetting device is an indispensable device in the gene sequencing process.

[0003] To meet the requirements of high-throughput sequencing, a large amount of reagent preparation and transmission work needs to be completed. Therefore, the pipetting devices currently used on the market usually adopt multi-channel pipetting plunger pumps and are equipped with multi-well plate reagent kits to meet the liquid configuration of multiple bottles of reagents simultaneously. However, when single-bottle reagent individual liquid configuration is required, this pipetting device cannot be used, and an additional liquid preparation workstation often needs to be added for manual liquid preparation. This increases the detection cost and results in high costs for related gene sequencing detections. Summary of the Invention

[0004] The main object of the present invention is to propose a pipetting device for gene detection, aiming to reduce the detection cost of gene sequencing.

[0005] To achieve the above object, the pipetting device for gene detection proposed by the present invention includes:

[0006] A pipetting platform, on which a plurality of reagent containers are placed, and the reagent containers are used for placing liquids to be configured;

[0007] A pipetting device, suspended above the pipetting platform and movable between a plurality of the reagent containers. The pipetting device includes a mounting bracket, a single-channel injection pump and a multi-channel injection pump. The single-channel injection pump and the multi-channel injection pump are both fixed on the mounting bracket. The mounting bracket moves between a plurality of the reagent containers, and both the single-channel injection pump and the multi-channel injection pump are used for transferring the liquids to be configured between a plurality of the reagent containers.

[0008] Optionally, the pipetting device further includes a driving component. The single-channel injection pump and the multi-channel injection pump are respectively arranged on opposite sides of the mounting bracket, and the driving component drives the single-channel injection pump and the multi-channel injection pump to reciprocally slide on the mounting bracket.

[0009] Optionally, a sliding seat is slidably mounted on the mounting bracket. The single-flux injection pump and the multi-flux injection pump are both mounted on the sliding seat. The driving assembly drives the sliding seat to slide, and a sliding guide rail is provided between the sliding seat and the mounting bracket to guide the sliding of the sliding seat.

[0010] Optionally, the single-flux injection pump includes a first pump body, a first plunger, and a first nozzle. A first injection cavity is provided in the first pump body. One end of the first plunger is partially inserted into the first injection cavity and can slide in the first injection cavity. The other end of the first nozzle is partially inserted into the first injection cavity to suck or discharge the liquid to be configured.

[0011] The multi-flux injection pump includes a second pump body, a plurality of second plungers, and a plurality of second nozzles. A plurality of second injection cavities are provided in the second pump body. One end of the second plunger is partially inserted into the second injection cavity and can slide in the second injection cavity. The other end of the second nozzle is partially inserted into the second injection cavity to suck or discharge the liquid to be configured.

[0012] Optionally, a seal is sleeved between the first plunger and the cavity wall at the opening of the first injection cavity, and between the second plunger and the cavity wall at the opening of the second injection cavity; and / or,

[0013] The first nozzle and the first injection cavity, and the second nozzle and the second injection cavity are in interference fit.

[0014] Optionally, the single-flux injection pump further includes a first driving motor and a first plunger support plate. One end of the first plunger away from the first injection cavity is connected to the first plunger support plate, and the first driving motor drives the first plunger to reciprocate through the first plunger support plate;

[0015] The multi-flux injection pump further includes a second driving motor and a second plunger support plate. One end of the second plunger away from the second injection cavity is connected to the second plunger support plate, and the second driving motor drives the second plunger to reciprocate through the second plunger support plate.

[0016] Optionally, the single-flux injection pump further includes a first nozzle withdrawal assembly for withdrawing the first nozzle from the first injection cavity;

[0017] The multi-flux injection pump further includes a second nozzle withdrawal assembly for withdrawing the second nozzle from the second injection cavity.

[0018] Optionally, the first gun-retracting assembly comprises a first gun-retracting plate and a first gun-retracting rod connected to each other, the first gun-retracting rod is penetrated through the first pump body, the first plunger support plate is provided with a first gun-retracting portion, the first gun-retracting portion pushes the first gun-retracting assembly to move, the first gun-retracting plate is sleeved on the first gun head, and a first blocking member is fixed to an end of the first gun head away from the first pump body, the first blocking member is used to prevent the first gun head from escaping from the first gun-retracting plate when the gun is retracted;

[0019] The second gun withdrawal assembly includes a second gun withdrawal plate and a second gun withdrawal rod that are connected to each other. The second gun withdrawal rod is inserted into the second pump body. The second plunger support plate is provided with a second gun withdrawal portion. The second gun withdrawal portion pushes the second gun withdrawal assembly to move. The second gun withdrawal plate is sleeved on the second gun head, and a second blocking member is fixed to the end of the second gun head that is away from the second pump body. The second blocking member is used to prevent the second gun head from escaping from the second gun withdrawal plate when the gun is withdrawn.

[0020] Optionally, the first gun head comprises a first inner gun head and a first outer gun head, the first outer gun head is plugged into the first mounting cavity, the first inner gun head is partially sleeved in the first outer gun head, and a first capacitor cavity is formed between the first inner gun head and the first outer gun head to form a capacitor to monitor the distance from the first gun head to the liquid surface of the liquid to be configured;

[0021] The second gun head includes a second inner gun head and a second outer gun head, the second outer gun head is inserted into the second mounting cavity, the second inner gun head is partially sleeved in the second outer gun head, and a second capacitor cavity is formed between the second inner gun head and the second outer gun head to form a capacitor to monitor the liquid level distance from the second gun head to the liquid to be configured.

[0022] Optionally, a pressure sensor is provided in the first installation cavity and / or the second installation cavity to detect pressure changes in the first installation cavity and / or the second installation cavity.

[0023] The technical solution of the present invention is to set up a pipetting platform, on which a plurality of reagent containers are placed, and the reagent containers are used to place the liquid to be configured. The pipetting device is suspended above the pipetting platform and can be moved between the plurality of reagent containers. The pipetting device includes a mounting bracket, a single-flux injection pump and a multi-flux injection pump. The mounting bracket is fixed with a single-flux injection pump for satisfying the liquid configuration of a single bottle of reagent alone, and a multi-flux injection pump for satisfying the liquid configuration of multiple bottles of reagents at the same time. Both the single-flux injection pump and the multi-flux injection pump are used to transfer the liquid to be configured between multiple reagent containers. Thereby, the pipetting device can satisfy both the liquid configuration of a single bottle of reagent alone and the liquid configuration of multiple bottles of reagents at the same time, thereby reducing the detection cost of gene sequencing. Brief Description of the Drawings

[0024] In order to more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the following will briefly introduce the drawings required for the description of the embodiments or the prior art. Obviously, the drawings in the following description are only some embodiments of the present invention. For those of ordinary skill in the art, without creative efforts, other drawings can be obtained based on the structures shown in these drawings.

[0025] Figure 1 Structural schematic diagram of an embodiment of a pipetting device for gene detection of the present invention;

[0026] Figure 2 is Figure 1 Structural schematic diagram of the pipetting device in the pipetting device;

[0027] Figure 3 is Figure 2 Structural schematic diagram of the mounting bracket in the pipetting device;

[0028] Figure 4 is Figure 2 Structural schematic diagram of the single-channel injection pump in the pipetting device;

[0029] Figure 5 is Figure 4 Side cross-sectional view of the single-channel injection pump;

[0030] Figure 6 is Figure 2 Structural schematic diagram of the single-channel injection pump in the pipetting device;

[0031] Figure 7 is Figure 6 Side cross-sectional view of the single-channel injection pump;

[0032] Figure 8 is Figure 4 Cross-sectional view of the first nozzle in the single-channel injection pump.

[0033] Explanation of the reference numerals in the drawings:

[0034]

[0035]

[0036] The realization of the object, functional characteristics and advantages of the present invention will be further described with reference to the embodiments and the drawings. Detailed Embodiments

[0037] Next, the technical solutions in the embodiments of the present invention will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.

[0038] It should be noted that if there are directional indications (such as up, down, left, right, front, back...) involved in the embodiments of the present invention, then such directional indications are only used to explain the relative positional relationship, movement conditions, etc. between components in a specific posture (as shown in the accompanying drawings). If this specific posture changes, then the directional indications will also change accordingly.

[0039] In addition, if there are descriptions involving "first", "second", etc. in the embodiments of the present invention, then such descriptions of "first", "second", etc. are only for descriptive purposes and cannot be understood as indicating or implying their relative importance or implicitly indicating the quantity of the indicated technical features. Thus, the features defined with "first" and "second" may explicitly or implicitly include at least one such feature. In addition, the meaning of "and / or" appearing throughout the text is that it includes three parallel solutions. Taking "A and / or B" as an example, it includes solution A, or solution B, or a solution where A and B are satisfied simultaneously. In addition, the technical solutions between various embodiments can be combined with each other, but it must be based on the fact that those of ordinary skill in the art can implement them. When the combination of technical solutions results in contradictions or cannot be implemented, it should be considered that such a combination of technical solutions does not exist and is not within the protection scope required by the present invention.

[0040] The present invention provides a pipetting device 100 for gene detection.

[0041] In the embodiments of the present invention, as Figures 1 to 2 shown, the pipetting device 100 for gene detection includes a pipetting platform 110 and a pipetting device 120. A plurality of reagent containers 111 are placed on the pipetting platform 110, and the reagent containers 111 are used for placing the liquid to be configured. The pipetting device 120 is suspended above the pipetting platform 110 and can move between a plurality of reagent containers 111. The pipetting device 120 includes a mounting bracket 130, a single-channel injection pump 140, and a multi-channel injection pump 150. Both the single-channel injection pump 140 and the multi-channel injection pump 150 are fixed on the mounting bracket 130. The mounting bracket 130 moves between a plurality of reagent containers 111, and both the single-channel injection pump 140 and the multi-channel injection pump 150 are used to transfer the liquid to be configured between a plurality of reagent containers 111.

[0042] Specifically, the pipetting platform 110 serves as the main liquid preparation platform. Multiple reagent containers 111, as well as the replaceable first pipette tip 143 and second pipette tip 153, etc., are all placed on the pipetting platform 110 to facilitate the preparation of the liquid to be prepared and the replacement of the first pipette tip 143 and the second pipette tip 153. And the reagent container 111 includes multiple multi-well plate containers and multiple reagent bottles, etc. The multiple multi-well plate containers and multiple reagent bottles are arranged on the pipetting platform 110 in a preset manner. The pipetting device 120 is suspended above the pipetting platform 110 and can move between multiple reagent containers 111, so as to complete the transfer of the liquid to be prepared in different reagent containers 111, and finally obtain the required solution to be detected. The pipetting device 120 is suspended above the pipetting platform 110 through a mounting frame, and at least one guide rail and a driving device are preset on the mounting frame. The driving device drives the pipetting device 120 to move on the guide rail, so as to complete the transfer of the liquid to be prepared. Generally, a single-channel syringe pump 140 needs to be used in combination with a reagent bottle or a reagent kit, while a multi-channel syringe pump 150 needs to be used in combination with a preset multi-well plate container, and the two cannot be interchanged. Therefore, the existing pipetting equipment 100 on the market often can only prepare liquids from multiple reagent bottles at the same time. However, when it is necessary to prepare the liquid of a single reagent bottle separately, the pipetting equipment 100 cannot be used, and often an additional liquid preparation workstation needs to be added for manual liquid preparation. Therefore, the cost of reagent preparation is greatly increased, thus increasing the cost of gene detection. The mounting bracket 130 is fixed with both a single-channel syringe pump 140 to meet the separate liquid preparation of a single reagent bottle and a multi-channel syringe pump 150 to meet the simultaneous liquid preparation of multiple reagent bottles. Thus, the pipetting equipment 100 can simultaneously meet the separate liquid preparation of a single reagent bottle and the simultaneous liquid preparation of multiple reagent bottles, thereby reducing the detection cost of gene sequencing. And optocouplers are installed between the mounting bracket 130 and the single-channel syringe pump 140, and / or between the mounting bracket 130 and the multi-channel syringe pump 150, so as to monitor the movement positions of the single-channel syringe pump 140 relative to the mounting bracket 130 and / or the multi-channel syringe pump 150 relative to the mounting bracket 130.

[0043] The technical solution of the present invention is achieved by providing a pipetting platform 110, on which a plurality of reagent containers 111 are placed. The reagent containers 111 are used for holding the liquids to be configured. The pipetting device 120 is suspended above the pipetting platform 110 and can move between the plurality of reagent containers 111. The pipetting device 120 includes a mounting bracket 130, a single-channel injection pump 140, and a multi-channel injection pump 150. The single-channel injection pump 140 is fixed to the mounting bracket 130 to meet the individual liquid configuration of a single reagent bottle, and the multi-channel injection pump 150 is also fixed to the mounting bracket 130 to meet the simultaneous liquid configuration of multiple reagent bottles. Both the single-channel injection pump 140 and the multi-channel injection pump 150 are used to transfer the liquids to be configured between the plurality of reagent containers 111. Thus, the pipetting device 100 can simultaneously meet the individual liquid configuration of a single reagent bottle and the simultaneous liquid configuration of multiple reagent bottles, thereby reducing the detection cost of gene sequencing.

[0044] Referring to Figures 1 to 3 , in one embodiment, the pipetting device 120 further includes a driving assembly 131. The single-channel injection pump 140 and the multi-channel injection pump 150 are respectively disposed on opposite sides of the mounting bracket 130, and the driving assembly 131 drives the single-channel injection pump 140 and the multi-channel injection pump 150 to reciprocate on the mounting bracket 130. Specifically, the pipetting device 120 is driven by a driving device to move horizontally above the pipetting platform 110, so that the mounting bracket 130 drives the single-channel injection pump 140 and the multi-channel injection pump 150 to move horizontally. The driving assembly 131 can drive the single-channel injection pump 140 and the multi-channel injection pump 150 to move vertically relative to each other, so that the single-channel injection pump 140 and the multi-channel injection pump 150 can complete the transfer of the liquids to be configured. The single-channel injection pump 140 and the multi-channel injection pump 150 are respectively disposed on opposite sides of the mounting bracket 130, so that their movements do not interfere with each other. Of course, in other embodiments, the single-channel injection pump 140 and the multi-channel injection pump 150 can also be arranged side by side, or the single-channel injection pump 140 and the multi-channel injection pump 150 can be arranged at an angle.

[0045] Further, a sliding seat 132 is slidably mounted on the mounting bracket 130. The single-flux injection pump 140 and the multi-flux injection pump 150 are both mounted on the sliding seat 132. The driving assembly 131 drives the sliding seat 132 to slide, and a sliding guide rail 133 is provided between the sliding seat 132 and the mounting bracket 130 to guide the sliding of the sliding seat 132. Specifically, the driving assembly 131 can drive the sliding seat 132 to slide vertically. The single-flux injection pump 140 and the multi-flux injection pump 150 are both mounted on the sliding seat 132, so that the sliding seat 132 drives the single-flux injection pump 140 and the multi-flux injection pump 150 to move. In the actual working process, generally, the liquid preparation of a single reagent bottle alone and the liquid preparation of multiple reagent bottles simultaneously will not be carried out at the same time, that is, the single-flux injection pump 140 and the multi-flux injection pump 150 will not work simultaneously. Therefore, using the same driving assembly 131 to drive the single-flux injection pump 140 and the multi-flux injection pump 150 to move can meet the requirements and is more economical. In another embodiment, two sets of driving assemblies 131 can also be used to drive the single-flux injection pump 140 and the multi-flux injection pump 150 to move respectively. At this time, the sliding seat 132 should also be correspondingly set to two, which respectively drive the single-flux injection pump 140 and the multi-flux injection pump 150 to move independently. And to ensure the smooth movement of the sliding seat 132, a sliding guide rail 133 is provided between the sliding seat 132 and the mounting bracket 130 to guide the sliding of the sliding seat 132.

[0046] In one embodiment, with reference to Figure 4 and Figure 5 , the single-flux injection pump 140 includes a first pump body 141, a first plunger 142 and a first nozzle 143. A first injection cavity 144 is provided in the first pump body 141. One end of the first plunger 142 is partially inserted into the first injection cavity 144 and can slide in the first injection cavity 144. One end of the first nozzle 143 is partially inserted into the other end of the first injection cavity 144 to suck or discharge the liquid to be prepared. Specifically, a first injection cavity 144 is formed in the first pump body 141 for storing the liquid to be prepared. The first plunger 142 is inserted from the end far away from the reagent container 111. One end of the first nozzle 143 is partially inserted into the other end of the first injection cavity 144. By the movement of one end of the first plunger 142 inserted into the first injection cavity 144 in the first injection cavity 144, the pressure in the first injection cavity 144 is changed, so that the first nozzle 143 sucks or discharges the liquid to be prepared.

[0047] And to increase the sealing performance of the first injection cavity 144, a sealing member 145 is sleeved between the first plunger 142 and the cavity wall at the opening of the first injection cavity 144.

[0048] In another embodiment, the first nozzle 143 and the first injection chamber 144 are in interference fit. Specifically, here the first nozzle 143 and the first injection chamber 144 are in a slight interference fit, so that the first nozzle 143 can be clamped in the first injection chamber 144 to improve the sealing performance of the first injection chamber 144. And it is also convenient to pull out the first nozzle 143 from the first injection chamber 144, thus facilitating the replacement of the first nozzle 143.

[0049] With reference to Figure 6 and Figure 7 In one embodiment, the single-flux injection pump 140 includes a second pump body 151, a plurality of second plungers 152 and a plurality of second nozzles 153. A plurality of second injection chambers 154 are provided in the second pump body 151. One end of the second plunger 152 is partially inserted into the second injection chamber 154 and can slide in the second injection chamber 154. The other end of the second nozzle 153 is partially inserted into the second injection chamber 154 to suck or discharge the liquid to be configured. Specifically, the second injection chamber 154 is opened in the second pump body 151 for storing the liquid to be configured. The second plunger 152 is inserted from the end far away from the reagent container 111. The other end of the second nozzle 153 is partially inserted into the second injection chamber 154. By the movement of one end of the second plunger 152 inserted into the second injection chamber 154 in the second injection chamber 154, the pressure in the second injection chamber 154 is changed, so that the second nozzle 153 sucks or discharges the liquid to be configured. In this embodiment, there are 8 second injection chambers 154 in the second pump body 151, and the number of holes in the multi-plate hole container used should also be a multiple of 8. Of course, in other embodiments, the number of second injection chambers 154 provided in the second pump body 151 can also be greater than 8, or less than or equal to 8 and greater than or equal to 2, and the number of holes in the multi-plate hole container used is set correspondingly.

[0050] And to increase the sealing performance of the second injection chamber 154, a sealing member 145 is sleeved between the second plunger 152 and the chamber wall at the opening of the second injection chamber 154.

[0051] In another embodiment, the second nozzle 153 and the second injection chamber 154 are in interference fit. Specifically, here the second nozzle 153 and the second injection chamber 154 are in a slight interference fit, so that the second nozzle 153 can be clamped in the second injection chamber 154 to improve the sealing performance of the second injection chamber 154. And it is also convenient to pull out the second nozzle 153 from the second injection chamber 154, thus facilitating the replacement of the second nozzle 153.

[0052] Again with reference to Figure 4 and Figure 5, in one embodiment, the single flux injection pump 140 further includes a first driving motor 146 and a first plunger carrier 147. One end of the first plunger 142 away from the first injection chamber 144 is connected to the first plunger carrier 147, and the first driving motor 146 drives the first plunger 142 to reciprocate through the first plunger carrier 147. Specifically, the first driving motor 146 drives the first plunger 142 to move through the first plunger carrier 147. In this embodiment, the first driving motor 146 drives the first plunger carrier 147 to move through a screw. One end of the first plunger 142 away from the first injection chamber 144 is connected to the first plunger carrier 147, so that the first plunger carrier 147 drives the first plunger 142 to move, thereby realizing liquid suction and liquid discharge.

[0053] Referring again to Figure 6 and Figure 7 , the multi-flux injection pump 150 further includes a second driving motor 155 and a second plunger carrier 156. One end of the second plunger 152 away from the second injection chamber 154 is connected to the second plunger carrier 156, and the second driving motor 155 drives the second plunger 152 to reciprocate through the second plunger carrier 156. Specifically, the second driving motor 155 drives the second plunger 152 to move through the second plunger carrier 156. In this embodiment, the second driving motor 155 drives the second plunger carrier 156 to move through a screw. One end of the second plunger 152 away from the second injection chamber 154 is connected to the second plunger carrier 156, so that the second plunger carrier 156 drives the second plunger 152 to move, thereby realizing liquid suction and liquid discharge.

[0054] To reduce the occurrence of reagent contamination, after the single flux injection pump 140 completes one reagent transfer, the first nozzle 143 needs to be replaced. To facilitate the replacement of the first nozzle 143, in one embodiment, referring to Figure 4 and Figure 5 , the single flux injection pump 140 further includes a first nozzle withdrawal assembly 160, and the first nozzle withdrawal assembly 160 is used to pull out the first nozzle 143 from the first injection chamber 144. Thus, it assists the single flux injection pump 140 to replace the first nozzle 143, thereby realizing the automatic replacement of the first nozzle 143 by the liquid transfer device 100 and improving the automation degree of the liquid transfer device 100.

[0055] Further, the first gun-retracting assembly 160 includes a first gun-retracting plate 161 and a first gun-retracting rod 162 connected to each other, the first gun-retracting rod 162 is inserted into the first pump body 141, the first plunger support plate 147 is provided with a first gun-retracting portion 147a, the first gun-retracting portion 147a pushes the first gun-retracting assembly 160 to move, the first gun-retracting rod 162 is sleeved on the first gun head 143, and a first blocking member is fixed to one end of the first gun head 143 away from the first pump body 141, the first blocking member is used to prevent the first gun head 143 from escaping from the first gun-retracting plate 161 when the gun is retracted. Specifically, the first gun-retracting plate 161 is sleeved on the first gun head 143, the first gun-retracting rod 162 is connected to the first gun-retracting plate 161, and is arranged at an angle. A through hole is opened on the first pump body 141, and one end of the first gun-retracting rod 162 away from the first gun-retracting plate 161 passes through the through hole and partially protrudes from the through hole. Because the first plunger support plate 147 moves up and down, and it has a first gun withdrawal portion 147a, the first gun withdrawal portion 147a is located above the first gun withdrawal rod 162. When the first plunger support plate 147 moves to a certain position, the first gun withdrawal portion 147a abuts against the first gun withdrawal rod 162, and pushes the first gun withdrawal rod 162 to move, thereby driving the first gun withdrawal plate 161 to withdraw the gun. A first blocking member is fixed to the end of the first gun head 143 away from the first pump body 141, and the first blocking member abuts against the side of the first gun withdrawal plate 161 away from the first pump body 141, thereby blocking the first gun head 143 from escaping from the first gun withdrawal plate 161 when withdrawing the gun. The first gun withdrawal plate 161 drives the first gun head 143 to be pulled out of the first injection cavity 144. And placed on the pipetting platform 110. Then move the pipetting device 120 to the first gun tip 143 to be replaced, replace the uncontaminated first gun tip 143, and then perform the next pipetting. The first gun retracting plate 161 and the first gun retracting rod 162 can be integrally formed, or fastened by threaded connection, clamping, bonding or riveting. In order to improve the stability during gun retracting, the first gun retracting rod 162 has two, which are respectively arranged at the opposite ends of the first gun retracting plate 161.

[0056] In order to reduce the occurrence of reagent contamination, the multi-flux injection pump 150 needs to replace the second gun tip 153 after completing a reagent transfer. To facilitate the replacement of the second gun tip 153, in one embodiment, in combination with reference Figure 6 and Figure 7 The multi-flux syringe pump 150 further includes a second gun-retracting assembly 170, which is used to pull out the second gun tip 153 from the second injection cavity 154. This assists the single-flux syringe pump 140 in replacing the second gun tip 153, thereby realizing automatic replacement of the second gun tip 153 by the pipetting device 100, thereby improving the automation of the device.

[0057] Further, the second gun withdrawal assembly 170 includes a second gun withdrawal plate 171 and a second gun withdrawal rod 172 connected to each other, the second gun withdrawal rod 172 is inserted into the second pump body 151, the second plunger support plate 156 is provided with a second gun withdrawal portion 156a, the second gun withdrawal portion 156a pushes the second gun withdrawal assembly 170 to move, the second gun withdrawal rod 172 is sleeved on the second gun head 153, and a second blocking member is fixed to one end of the second gun head 153 away from the second pump body 151, and the second blocking member is used to prevent the second gun head 153 from escaping from the second gun withdrawal plate 171 when the gun is withdrawn. Specifically, the second gun withdrawal plate 171 is sleeved on the second gun head 153, and the second gun withdrawal rod 172 is connected to the second gun withdrawal plate 171 and is arranged at an angle. A through hole is opened on the second pump body 151, and one end of the second gun withdrawal rod 172 away from the second gun withdrawal plate 171 passes through the through hole and partially protrudes from the through hole. Because the second plunger support plate 156 moves up and down, and it has a second gun withdrawal portion 156a, the second gun withdrawal portion 156a is located above the second gun withdrawal rod 172. When the second plunger support plate 156 moves to a certain position, the second gun withdrawal portion 156a abuts against the second gun withdrawal rod 172, and pushes the second gun withdrawal rod 172 to move, thereby driving the second gun withdrawal plate 171 to withdraw the gun. A second blocking member is fixed to the end of the second gun head 153 away from the second pump body 151, and the second blocking member abuts against the side of the second gun withdrawal plate 171 away from the second pump body 151, thereby blocking the second gun head 153 from escaping from the second gun withdrawal plate 171 when withdrawing the gun. The second gun withdrawal plate 171 drives the second gun head 153 to be pulled out of the second injection cavity 154. And placed on the pipetting platform 110. Then move the pipetting device 120 to the second gun tip 153 to be replaced, replace the uncontaminated second gun tip 153, and then perform the next pipetting. The second gun retracting plate 171 and the second gun retracting rod 172 can be integrally formed, or fastened by threaded connection, clamping, bonding or riveting. In order to improve the stability during gun retracting, the second gun retracting rod 172 has two, which are respectively arranged at the opposite ends of the second gun retracting plate 171.

[0058] To monitor the distance between the first gun tip 143 and the liquid surface of the liquid to be prepared, in one embodiment, in combination with reference Figure 4 , Figure 5 and Figure 8, the first nozzle 143 includes a first inner nozzle 143a and a first outer nozzle 143b. The first outer nozzle 143b is inserted into the first installation cavity. The first inner nozzle 143a is partially sleeved in the first outer nozzle 143b, and a first capacitance cavity 143c is formed between the first inner nozzle 143a and the first outer nozzle 143b to form a capacitance for monitoring the liquid level distance from the first nozzle 143 to the liquid to be configured. Specifically, the first outer nozzle 143b is inserted into the first installation cavity, and the first inner nozzle 143a is partially sleeved in the first outer nozzle 143b, so that the two are spaced apart in the radial direction, abutted in the axial direction, and isolated by an isolation member 143e. Thus, the first capacitance cavity 143c is formed. When the first inner nozzle 143a contacts the liquid, the capacitance in the first nozzle 143 changes, and thus a signal is transmitted to the control system, causing the first plunger 142 to move for liquid extraction. And to enhance the capacitance in the first capacitance cavity 143c and amplify the capacitance change in the first nozzle 143, the first capacitance cavity 143c is filled with a filling material 143d. And to reduce the loss of the liquid to be configured on the outer wall of the first nozzle 143, in one embodiment, when the single-flux injection pump 140 absorbs the liquid, it moves downward with the drop of the liquid for liquid level following.

[0059] To monitor the liquid level distance from the second nozzle 153 to the liquid to be configured, in one embodiment, the second nozzle 153 includes a second inner nozzle 153a and a second outer nozzle 153b. The second outer nozzle 153b is inserted into the second installation cavity. The second inner nozzle 153a is partially sleeved in the second outer nozzle 153b, and a second capacitance cavity 153c is formed between the second inner nozzle 153a and the second outer nozzle 153b to form a capacitance for monitoring the liquid level distance from the second nozzle 153 to the liquid to be configured. Specifically, the second outer nozzle 153b is inserted into the second installation cavity, and the second inner nozzle 153a is partially sleeved in the second outer nozzle 153b, so that the two are spaced apart in the radial direction, abutted in the axial direction, and isolated by an isolation member 143e. Thus, the second capacitance cavity 153c is formed. When the second inner nozzle 153a contacts the liquid, the capacitance in the second nozzle 153 changes, and thus a signal is transmitted to the control system, causing the second plunger 152 to move for liquid extraction. And to enhance the capacitance in the second capacitance cavity 153c and amplify the capacitance change in the second nozzle 153, the second capacitance cavity 153c is filled with a filling material 143d.

[0060] In one embodiment, a pressure sensor 148 is provided in both the first installation cavity and / or the second installation cavity to detect the pressure change in the first installation cavity and / or the second installation cavity. Specifically, the pressure sensor 148 can effectively monitor the pressure change in the first installation cavity and / or the second installation cavity, and judge whether there is liquid leakage in the first installation cavity and / or the second installation cavity during the liquid transfer process according to the pressure change. Therefore, the pressure sensor 148 is installed on the inner wall of the first installation cavity and / or the second installation cavity.

[0061] The working process of the single-channel injection pump 140 in the pipetting device 100 for gene detection is as follows:

[0062] The driving mechanism drives the pipetting device 100 to move above the first nozzle 143, and then the driving component 131 drives the single-channel injection pump 140 to move downward relative to the mounting bracket 130, and inserts the first nozzle 143 into the first injection chamber 144, so that the first nozzle 143 is slightly interference-fitted with the first injection chamber 144. Then the driving component 131 drives the single-channel injection pump 140 to move upward relative to the mounting bracket 130, and the driving mechanism drives the pipetting device 100 to move above the liquid to be configured. Then the driving component 131 drives the single-channel injection pump 140 to move downward relative to the mounting bracket 130, and the liquid level detector is turned on until it contacts the liquid to be configured. The capacitance in the first nozzle 143 changes. After being detected by the liquid level detector, the first nozzle 143 sucks the liquid to be configured, and during the liquid suction process, the first nozzle 143 moves downward following the liquid level as the liquid drops. After the suction is completed, the driving component 131 drives the single-channel injection pump 140 to move upward relative to the mounting bracket 130, and the driving mechanism drives the pipetting device 100 to move above another reagent container 111 for solution preparation. Then the driving mechanism drives the pipetting device 100 to move to a preset position, so that the first driving motor 146 drives the first plunger plate 147 to move. Subsequently, the first gun-retracting portion 147a on the first plunger plate 147 pushes the first gun-retracting rod 162 to move, so that the first gun-retracting plate 161 withdraws the first nozzle 143 from the first injection chamber 144. Then the above steps are repeated to complete single-channel pipetting.

[0063] The working process of the multi-channel injection pump 150 in the pipetting device 100 for gene detection is as follows:

[0064] The driving mechanism drives the liquid transfer device 100 to move above the second nozzle 153. Then, the driving component 131 drives the single-flux injection pump 140 to move downward relative to the mounting bracket 130, and inserts the second nozzle 153 into the second injection chamber 154, so that the second nozzle 153 is in a slightly interference fit with the second injection chamber 154. Then, the driving component 131 drives the multi-flux injection pump 150 to move upward relative to the mounting bracket 130, and the driving mechanism drives the liquid transfer device 100 to move above the liquid to be configured. Then, the driving component 131 drives the multi-flux injection pump 150 to move downward relative to the mounting bracket 130 until it contacts the liquid to be configured. The second nozzle 153 sucks the liquid to be configured. After the sucking is completed, the driving component 131 drives the single-flux injection pump 140 to move upward relative to the mounting bracket 130, and the driving mechanism drives the liquid transfer device 100 to move above another reagent container 111 for solution preparation. Then, the driving mechanism drives the liquid transfer device 100 to move to a preset position, so that the second driving motor 155 drives the second plunger support plate 156 to move. Subsequently, the second gun-retracting portion 156a on the second plunger support plate 156 pushes the second gun-retracting rod 172 to move, so that the second gun-retracting plate 171 withdraws the second nozzle 153 from the second injection chamber 154. Then, the above steps are repeated to complete multi-flux liquid transfer.

[0065] The above are only the preferred embodiments of the present invention, and do not limit the patent scope of the present invention. Any equivalent structural transformation made by using the description and drawings of the present invention under the inventive concept of the present invention, or any direct / indirect application in other related technical fields is included in the patent protection scope of the present invention.

Claims

1. A pipetting device for gene detection, characterized in that, it includes: A pipetting platform on which a plurality of reagent containers are placed, and the reagent containers are used for placing liquids to be configured; A pipetting device is suspended above the pipetting platform and can move between a plurality of the reagent containers. The pipetting device includes a mounting bracket, a single-channel injection pump, and a multi-channel injection pump. The single-channel injection pump and the multi-channel injection pump are both fixed on the mounting bracket. The mounting bracket moves between a plurality of the reagent containers, and both the single-channel injection pump and the multi-channel injection pump are used for transferring the liquids to be configured between a plurality of the reagent containers; The pipetting device further includes a driving component. The single-channel injection pump and the multi-channel injection pump are respectively arranged on opposite sides of the mounting bracket, and the driving component drives the single-channel injection pump and the multi-channel injection pump to slide back and forth on the mounting bracket; The single-channel injection pump includes a first pump body, a first plunger, and a first nozzle. A first injection chamber is provided in the first pump body. One end of the first plunger is partially inserted into the first injection chamber and can slide in the first injection chamber. The other end of the first nozzle is partially inserted into the first injection chamber to suck or discharge the liquid to be configured; The single-channel injection pump further includes a first driving motor and a first plunger support plate. One end of the first plunger away from the first injection chamber is connected to the first plunger support plate, and the first driving motor drives the first plunger to slide back and forth through the first plunger support plate; A first nozzle retracting portion is provided on the first plunger support plate.

2. The pipetting device for gene detection according to claim 1, characterized in that, A sliding seat is slidably mounted on the mounting bracket. The single-channel injection pump and the multi-channel injection pump are both mounted on the sliding seat. The driving component drives the sliding seat to slide, and a sliding guide rail is provided between the sliding seat and the mounting bracket to guide the sliding of the sliding seat.

3. The pipetting device for gene detection according to claim 1, characterized in that, The multi-channel injection pump includes a second pump body, a plurality of second plungers, and a plurality of second nozzles. A plurality of second injection chambers are provided in the second pump body. One end of the second plunger is partially inserted into the second injection chamber and can slide in the second injection chamber. The other end of the second nozzle is partially inserted into the second injection chamber to suck or discharge the liquid to be configured.

4. The pipetting device for gene detection according to claim 3, characterized in that, Sealing members are sleeved between the first plunger and the chamber wall at the opening of the first injection chamber and between the second plunger and the chamber wall at the opening of the second injection chamber; and / or, The first nozzle and the first injection chamber and the second nozzle and the second injection chamber are in interference fit.

5. The pipetting device for gene detection according to claim 3, characterized in that, The multi-flux injection pump also includes a second driving motor and a second plunger support plate, one end of the second plunger away from the second injection chamber is connected to the second plunger support plate, and the second driving motor drives the second plunger to slide reciprocatingly through the second plunger support plate.

6. The liquid transfer device for gene detection according to claim 5, It is characterized in that The single-flux injection pump further includes a first gun withdrawal assembly, which is used to pull the first gun tip out of the first injection cavity; The multi-flux injection pump further includes a second gun withdrawal assembly, and the second gun withdrawal assembly is used to pull the second gun tip out of the second injection cavity.

7. The liquid transfer device for gene detection according to claim 6, It is characterized in that The first gun-retracting assembly comprises a first gun-retracting plate and a first gun-retracting rod connected to each other, the first gun-retracting rod is penetrated through the first pump body, the first gun-retracting part pushes the first gun-retracting assembly to move, the first gun-retracting plate is sleeved on the first gun head, and a first blocking member is fixed to an end of the first gun head away from the first pump body, the first blocking member is used to prevent the first gun head from escaping from the first gun-retracting plate when the gun is retracted; The second gun withdrawal assembly includes a second gun withdrawal plate and a second gun withdrawal rod that are connected to each other. The second gun withdrawal rod is inserted into the second pump body. The second plunger support plate is provided with a second gun withdrawal portion. The second gun withdrawal portion pushes the second gun withdrawal assembly to move. The second gun withdrawal plate is sleeved on the second gun head, and a second blocking member is fixed to the end of the second gun head that is away from the second pump body. The second blocking member is used to prevent the second gun head from escaping from the second gun withdrawal plate when the gun is withdrawn.

8. The liquid transfer device for gene detection according to claim 3, It is characterized in that The first gun head comprises a first inner gun head and a first outer gun head, the first outer gun head is inserted into the first installation cavity, the first inner gun head is partially sleeved in the first outer gun head, and a first capacitor cavity is formed between the first inner gun head and the first outer gun head to form a capacitor to monitor the distance from the first gun head to the liquid surface of the liquid to be configured; The second gun head includes a second inner gun head and a second outer gun head, the second outer gun head is inserted into the second installation cavity, the second inner gun head is partially sleeved in the second outer gun head, and a second capacitor cavity is formed between the second inner gun head and the second outer gun head to form a capacitor to monitor the distance from the second gun head to the liquid level of the liquid to be configured.

9. The liquid transfer device for gene detection according to claim 8, It is characterized in that A pressure sensor is disposed in the first installation cavity and / or the second installation cavity to detect pressure changes in the first installation cavity and / or the second installation cavity.

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