Hydrating cosmetic compositions containing hydrolyzed plant extracts

Abstract

A moisturizing cosmetic composition comprising hydrolyzed plant extracts. This invention relates to a moisturizing cosmetic composition comprising hydrolyzed extracts of malt, gardenia, and pansy. The cosmetic composition of this invention exhibits excellent effects on cellular and intercellular hydration. Furthermore, the cosmetic composition of this invention uses natural ingredients, thus it is harmless to the human body and exhibits excellent stability.

Description

Technical Field

[0001] This invention relates to a moisturizing cosmetic composition comprising hydrolyzed plant extracts, and more specifically, to a moisturizing cosmetic composition comprising hydrolyzed extracts of malt, gardenia Florida, and viola tricolor. Background Art

[0002] The skin not only has the largest surface area among human organs but is also the outermost organ, preventing the loss of water, electrolytes, and other biological components from the body while also preventing harmful substances from invading from the outside. The skin's structure consists of three layers from the outside: the epidermis, the dermis, and the subcutaneous adipose tissue.

[0003] The skin's moisture content is approximately 70% in the dermis, gradually decreasing towards the epidermis, where it ranges from about 10% to 30%. When the epidermal moisture content drops below 10%, the skin becomes rough and loses its protective function, leading to signs of aging. The epidermis consists of four layers: the stratum corneum, stratum granulosum, stratum spinosum, and stratum basale. The stratum corneum, as the outermost layer, regulates moisture evaporation and absorption, and acts as a barrier against the penetration of external substances such as chemicals, toxins, and bacteria. The stratum corneum is composed of flattened keratinocytes and intercellular lipids that fill the spaces between them, forming a thin lamellar layer. It is well known that the moisture content of the stratum corneum is a crucial factor determining the skin's elasticity and radiance.

[0004] Furthermore, healthy individuals possess high concentrations of natural moisturizing factor (NMF) in their keratinocytes, which helps the skin retain moisture. However, despite these functions, external environmental factors such as indoor heating, air pollution, and ultraviolet radiation lead to extrinsic and endogenous aging, while physical and chemical irritants like friction, shaving, and face washing cause the shedding of skin components, resulting in moisture imbalances and dry skin. Therefore, the necessity of skin hydrating agents has been recognized to prevent this, and various studies are underway to confirm their skin hydrating effects at the cellular level.

[0005] Existing technical documents

[0006] Patent Literature

[0007] Korean Patent No. 10-1987167 (issued on June 3, 2019) discloses a cosmetic composition for skin hydration and skin barrier strengthening, which comprises a complex extract of corn cob, malt and flaxseed treated with hydrolytic enzyme.

[0008] Korean Patent No. 10-1819060 (January 10, 2018) discloses a cosmetic composition for anti-oxidation, wrinkle improvement and whitening, which contains Huanglian Jiedu Decoction (Coptis chinensis, Phellodendron chinense, Scutellaria baicalensis and Gardenia jasminoides) ferment as an active ingredient.

[0009] Korean Patent No. 10-0858449 (September 8, 2008) discloses a cosmetic composition for moisturizing the skin and improving wrinkles, which contains a pansy extract stabilized by nanoliposomes. Summary of the Invention

[0010] Technical issues

[0011] This invention addresses the problems with chemical substances used in existing moisturizing cosmetic compositions, aiming to develop and provide a natural raw material cosmetic composition that is harmless to the human body, has excellent stability, and provides good moisturizing effects.

[0012] Solution to the problem

[0013] This invention provides a skin moisturizing cosmetic composition comprising hydrolyzed malt extract, hydrolyzed gardenia Florida extract, and hydrolyzed viola tricolor extract.

[0014] In the skin moisturizing cosmetic composition of the present invention, the moisturizing preferably includes any one or more selected from cellular moisturizing and intercellular moisturizing.

[0015] In the skin moisturizing cosmetic composition of the present invention, as an example, the above extraction can be performed by any one of solvent extraction, supercritical extraction and ultrasonic extraction.

[0016] In this case, as an example, the solvent extraction method described above can use any one of the following extraction solvents selected from water, anhydrous or aqueous lower alcohols having 1-4 carbon atoms, propylene glycol, butanediol, glycerol, acetone, ethyl acetate, chloroform, butyl acetate, diethyl ether, dichloromethane, hexane, or mixtures thereof.

[0017] The effects of the invention

[0018] The cosmetic composition of the present invention exhibits excellent effects in cellular and intercellular hydration. Furthermore, the cosmetic composition of the present invention uses natural raw materials, thus it is harmless to the human body and possesses excellent stability. Attached Figure Description

[0019] Figure 1The results were used to determine the toxicity of the extracts of Comparative Examples 1 to 3 or the mixture of Comparative Examples 4 to 6 and Example 1 to cells.

[0020] Figure 2 The results of the cell hydration effect were confirmed by adjusting the expression of aquaporin-3 by the extracts of Comparative Examples 1 to 3 or the mixture of Comparative Examples 4 to 6 and Example 1.

[0021] Figure 3 The results of the skin barrier hydration effect were confirmed by adjusting the expression of Claudin-1 in the extracts of Comparative Examples 1 to 3 or the mixture of Comparative Examples 4 to 6 and Example 1.

[0022] Figure 4 To show the results of moisture reduction rates of the extracts of Comparative Examples 1 to 3 or the mixtures of Comparative Examples 4 to 6 and Example 1.

[0023] Figure 5 The results were determined when the skin moisture content was measured using dosage form 1 and comparative dosage form 1.

[0024] Figure 6 The results were used to determine the amount of transdermal water loss when using dosage form 1 and comparative dosage form 1. Detailed Implementation

[0025] Maintaining adequate moisture content in the stratum corneum of the skin is crucial. Therefore, cosmetics often incorporate ingredients similar to sebum or moisturizing agents such as natural moisturizers (NMF) and polyols. For example, while water-soluble polyols with three or more hydroxyl groups (OHgroups) like glycerin and sorbitol exhibit excellent moisturizing power, their high viscosity can cause discomfort during use. Conversely, polyols with two hydroxyl groups, such as propylene glycol and 1,3-butanediol, can cause side effects on the skin. Furthermore, other natural moisturizing factors such as sodium pyrrolidone carboxylate (PCA-Na), sodium lactate, and urea have high electrolytic activity, which can affect the emulsification stability of cosmetics. While amino acids, collagen, and elastin also possess moisturizing abilities, their capacity is limited. Therefore, there is a need to develop safe natural cosmetic compositions that can enhance skin hydration.

[0026] In response, the present invention provides a skin moisturizing cosmetic composition comprising hydrolyzed malt extract, hydrolyzed gardenia Florida extract, and hydrolyzed viola tricolor extract.

[0027] Malt is produced by germinating barley (Hordeum vulgare Linn) to generate amylase, an enzyme that acts as maltase. It is hard, with buds less than 2 cm long and a yellow color. Malt is effective for indigestion caused by weak spleen and stomach, especially for indigestion caused by flour-based foods. It is also effective for insufficient milk production. It has a young bud on one end and a young root on the other, with a pale yellow surface and milky white endosperm. It is hard and brittle. Other names for malt include barley malt, barley sprout, barley hair, barley sprout, and millet malt.

[0028] Gardenia (Gardenia Florida) refers to the fruit or dried product of the gardenia tree, a member of the Rubiaceae family of evergreen shrubs. Gardenia trees grow naturally in the deep mountains of hot regions such as south-central Korea, China (including Taiwan), and Japan. The fruit is about 3.5 cm long, ripening in September and turning yellowish-red. It is used not only medicinally but also as a dye. Pharmacologically, it is known to be effective against diabetes, hypertension, insomnia, jaundice, urinary disorders, conjunctivitis, hematemesis, uterine bleeding, hematuria, and contusions. It also treats joint contusions and has detoxifying effects. Gardenia can be decocted alone or with other medicinal herbs, or ground into powder and mixed with water for application to the affected area.

[0029] Viola tricolor is an annual plant belonging to the Violaceae family of the order Parietal placentation, also known as the tricolor violet. It grows to a height of 10-15 cm, with stems that grow straight or extend laterally and are highly branched. The lower leaves are oval, while the upper leaves are slightly slender and shovel-shaped. The petioles have long and large stipules. From March to June, long flower stalks emerge from the leaf axils, each bearing a single flower at its end. The flowers have five petals and a diameter of 3-12 cm, with small, medium, and large whorls. Flowers come in white, yellow, and purple, but horticultural varieties exhibit variations in color, including solid colors, orange, brownish-blue, red, and blue. The fruit is a capsule, oval in shape.

[0030] In the skin-hydrating cosmetic composition of the present invention, the hydration preferably includes one or more selected from cellular hydration and intercellular hydration. In a skin-hydrating cosmetic composition comprising hydrolyzed malt extract, hydrolyzed gardenia extract, and hydrolyzed pansy extract in one embodiment of the present invention, excellent effects on skin cell and intercellular hydration were confirmed by confirming the expression of aquaporin-3 and claudin-1. Furthermore, it also exhibits excellent efficacy in hydration.

[0031] Therefore, various formulations with excellent skin hydration effects can be provided by using cosmetic compositions containing hydrolyzed extracts of malt, gardenia Florida, and viola tricolor as active ingredients.

[0032] On the one hand, it is well known that aquaporins (AQPs), as membrane proteins, are responsible for water transport in the cell membrane. They selectively regulate the water channels for water molecules to enter and exit the cell while interfering with the movement of ions and solutes. It is known that 13 AQPs (AQP-0 to AQP-12) exist in mammals. Among them, AQP-1, AQP-2, AQP-4, AQP-5, and AQP-8 mainly selectively transport water, but AQP-3, AQP-7, AQP-9, and AQP-10 not only transport water but also have the function of transporting glycerol and other small solutes. In particular, it has been reported that AQP-3 is expressed in keratinocytes of the basal layer of the epidermis. Activation of AQP-3 in keratinocytes transports water deeper into the skin, thus promising excellent skin hydration effects.

[0033] On the other hand, the skin's ability to retain moisture is closely related to the function of the skin barrier, which can be damaged by aging and external stimuli. Damage to the skin barrier directly leads to reduced skin moisture content and wrinkle formation, and extensive research is underway to address this issue. Among the key factors is the role of tight junctions (TJs) between keratinocytes, the main cells constituting the skin layer.

[0034] Tight junctions are responsible for cell adhesion, preventing water loss and the penetration of harmful substances from the body. Known tight junction proteins include occldin, claudin, and ZO-1, most of which are located between the granulosa cells of the epidermis. Claudin-1, a transmembrane protein, is composed of a ring structure that regulates intercellular spacing by tightly filling the gaps between cells, effectively blocking water movement. Recent research suggests that claudin-1 plays a crucial role in skin barrier function, influencing intercellular hydration.

[0035] In the skin-hydrating cosmetic composition of the present invention, as an example, the above-mentioned extraction can be performed by any one of the extraction methods selected from solvent extraction, supercritical extraction, and ultrasonic extraction. In this case, as an example, the solvent extraction method can use any one of the following extraction solvents selected from water, anhydrous or aqueous lower alcohols having 1-4 carbon atoms, propylene glycol, butylene glycol, glycerin, acetone, ethyl acetate, chloroform, butyl acetate, diethyl ether, dichloromethane, hexane, or mixtures thereof.

[0036] In particular, in one embodiment of the present invention, the excellent moisturizing effects of each of the hydrolyzed malt extract, hydrolyzed gardenia Florida extract, and hydrolyzed viola tricolor extract, as well as mixtures thereof, were confirmed, and it was confirmed that a very excellent moisturizing effect could be exhibited when the above extracts were mixed in the same proportion.

[0037] On one hand, in this invention, the above-mentioned mixed extract preferably comprises 0.0001 to 30.0% by weight relative to the total weight of the cosmetic composition. More preferably, it comprises 0.01 to 10% by weight relative to the total weight of the cosmetic composition. When the content of the mixed extract is less than 0.0001% by weight, skin hydration is minimal, and when it is greater than 30.0% by weight, there is no significant increase in effect with increasing content.

[0038] On the other hand, the cosmetic composition of the present invention may contain, in addition to the mixed extract of the present invention, ingredients commonly used in cosmetic compositions as active ingredients, such as antioxidants, stabilizers, solubilizers, vitamins, pigments and fragrances, as well as carriers.

[0039] The cosmetic compositions of the present invention can be formulated into any dosage form commonly prepared in the art, such as solutions, suspensions, emulsions, creams, gels, lotions, powders, soaps, surfactant-containing cleansers, oils, powder foundations, lotion foundations, wax foundations, masks, massage creams, and sprays, but are not limited thereto. More specifically, they can be formulated into softening lotions, nourishing lotions, nourishing creams, massage creams, serums, eye creams, cleansing balms, cleansing foams, makeup removers, masks, sprays, and powder forms.

[0040] When the dosage form of the cosmetic composition of the present invention is an ointment, cream, or gel, animal oil, vegetable oil, wax, paraffin, starch, tragacanth gum, cellulose derivatives, polyethylene glycol, silicone, bentonite, silica, talc, or zinc oxide may be used as a carrier component.

[0041] When the dosage form of the cosmetic composition of the present invention is a solution or an emulsion, a solvent, solubilizer, or emulsifier is used as a carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1,3-butanediol oil, glyceryl esters, polyethylene glycol, or fatty acid esters of sorbitan.

[0042] When the dosage form of the cosmetic composition of the present invention is a suspension, liquid diluents such as water, ethanol or propylene glycol can be used; suspending agents such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitan ester and polyoxyethylene dehydrated sorbitan ester; and carrier components such as microcrystalline cellulose, aluminum hydroxide, bentonite, agar or tragacanth gum.

[0043] When the cosmetic composition of the present invention is in the form of a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder can be used as a carrier component. Especially in the case of a spray, a propellant such as chlorofluorocarbon, propane / butane or dimethyl ether can be additionally included.

[0044] When the cosmetic composition of the present invention is a surfactant-containing cleanser, fatty alcohol sulfates, fatty alcohol ether sulfates, sulfosuccinate monoesters, hydroxyethyl sulfonates, imidazoline derivatives, methyl taurate, sarcosinates, fatty acid amide ether sulfates, cocamidopropyl betaine, fatty alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives, or ethoxylated glycerol fatty acid esters may be used as carrier components.

[0045] When the cosmetic composition of the present invention is a soap, a surfactant-containing cleanser, or a surfactant-free cleanser, it can be wiped off, peeled off, or washed off with water after being applied to the skin. Specific examples include liquid soap, powdered soap, solid soap, and oil soap; surfactant-containing cleansers include facial foam, makeup remover, makeup remover wipes, and cleansing masks; and surfactant-free cleansers include cleansing balms, cleansing lotions, cleansing water, and cleansing gels, but are not limited thereto.

[0046] The following will describe the structure of the present invention in detail through the following embodiments and experimental examples. However, the scope of protection of the present invention is not limited to the following embodiments and experimental examples, but also includes modifications of equivalent technical concepts.

[0047] Preparation Example 1: Preparation of Hydrolyzed Malt Extract

[0048] 100g of malt was crushed and added to 1000g of purified water. The mixture was then extracted with hot water at 100°C for 3 hours, followed by filtration through a 400-mesh filter cloth. The malt extract was then prepared by concentration under reduced pressure.

[0049] Then, 500g of 1M HCl solution was added to 5g of malt extract, and the mixture was stirred at 25°C for 3 hours. Then, it was neutralized with 1M NaOH solution to prepare "hydrolyzed malt extract".

[0050] Preparation Example 2: Preparation of hydrolyzed gardenia (Gardenia Florida) extract

[0051] 100g of gardenia (Gardenia Florida) was crushed and added to 1000g of purified water. The mixture was extracted with hot water at 100°C for 3 hours, and then filtered through a 400-mesh filter cloth. The "gardenia extract" was then prepared by concentration under reduced pressure.

[0052] Then, 500g of 1M HCl solution was added to 5g of gardenia extract, and the mixture was stirred at 25°C for 3 hours. Then, it was neutralized with 1M NaOH solution to prepare "hydrolyzed gardenia extract".

[0053] Preparation Example 3: Preparation of hydrolyzed Viola Tricolor extract

[0054] 100g of Viola Tricolor was crushed and added to 1000g of purified water. The mixture was extracted with hot water at 100°C for 3 hours, and then filtered through a 400-mesh filter cloth. The Viola Tricolor extract was then prepared by concentration under reduced pressure.

[0055] Subsequently, 500g of 1M HCl solution was added to 5g of pansy extract, and the mixture was stirred at 25°C for 3 hours. Then, it was neutralized with 1M NaOH solution to prepare "hydrolyzed pansy extract".

[0056] Example 1 and Comparative Examples 1 to 6: Preparation of mixtures of hydrolyzed malt extract, hydrolyzed gardenia Florida extract, and hydrolyzed viola tricolor extract.

[0057] The mixture of hydrolyzed malt extract, hydrolyzed gardenia Florida extract, and hydrolyzed viola tricolor extract prepared in Preparation Examples 1 to 3 above was mixed in the proportions shown in Table 1 below and dissolved in dimethyl sulfoxide (DMSO) at a concentration of 100 mg / ml for use in the following experiments.

[0058] Table 1

[0059] Element Example 1 Comparative Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 4 Comparative Example 5 Comparative Example 6 Hydrolyzed malt extract 1 3 - - 1.5 1.5 - Hydrolyzed Gardenia Extract 1 - 3 - 1.5 - 1.5 Hydrolyzed Pansy Extract 1 - - 3 - 1.5 1.5

[0060] Experiment Example 1: Determining Cell Viability

[0061] In this experiment, the toxicity of the extracts of Comparative Examples 1 to 3, or the mixture of Comparative Examples 4 to 6 and Example 1 to cells was determined. Cell viability was determined using the MTT (3-(4,5-dimethyl-2-thiazol)-2-5-diphenyltetrazolium bromide) reagent.

[0062] Specifically, human keratinocytes (HaCaT) were used at a concentration of 2 × 10⁻⁶. 4 Cells / well concentrations were dispensed into 96-well plates and cultured at 37°C and 5% CO2 for 24 hours. The culture medium was then removed, and the extracts of Comparative Examples 1 to 3, or the mixture of Comparative Examples 4 to 6 and Example 1, were serially diluted at different concentrations (1 μg / ml, 3 μg / ml, 10 μg / ml, 30 μg / ml, and 100 μg / ml) and cultured for 24 hours.

[0063] Then, 20 μl of MTT reagent dissolved at 5 mg / ml was added, and the mixture was reacted at 37 °C and 5% CO2 for 2 hours. After completely removing the culture medium, 100 μl of DMSO was added to the generated formazan and completely dissolved, and the absorbance was measured at 540 nm.

[0064] Experimental results showed that the mixture of Example 1 and the extracts of Comparative Examples 1 to 3, or the mixture of Comparative Examples 4 to 6, did not exhibit cytotoxicity at a concentration of 100 μg / ml. Figure 1 ). Figure 1 The results were used to determine the toxicity of the extracts of Comparative Examples 1 to 3 or the mixture of Comparative Examples 4 to 6 and Example 1 to cells.

[0065] Experiment Example 2: Confirming the cell hydration effect by confirming the expression of aquaporin-3.

[0066] To evaluate the hydration effect at the cellular level, the effects of extracts from Comparative Examples 1 to 3, or the mixture of Comparative Examples 4 to 6 and Example 1, on AQP-3, a channel involved in intracellular water absorption, were confirmed. Dexamethasone was used as a positive control.

[0067] Furthermore, when HaCaT cells were aliquoted into 6-well plates and filled to 80% capacity, the extracts of Comparative Examples 1 to 3, or the mixture of Comparative Examples 4 to 6 and Example 1, were treated in culture medium at a concentration of 100 μg / ml. The cells were then cultured for 24 hours and collected, and proteins were separated using 1× cell lysis buffer.

[0068] Proteins were then quantified using the BCA assay, and a measured amount of protein was electrophoresed on a 10% SDS-PAGE gel. The protein was then transferred to a PVDF membrane and blocked with 5% skim milk for 1 hour. Primary antibody (AQP-3, Santa Cruz, USA) was incubated overnight at 4°C, followed by HRP-conjugated secondary antibody incubation at room temperature for 2 hours. The bands were then washed with TBS / T and confirmed using an ECL reaction kit via a Chemi-Doc instrument. Quantification of each band was based on β-actin expression to calculate AQP-3 expression levels.

[0069] The experimental results showed that dexamethasone, as the positive control, increased AQP-3 expression by approximately 73.0%, while the mixture from Example 1, at a concentration of 100 μg / ml, increased AQP-3 expression by approximately 113.2%. The extract from Comparative Example 2 increased AQP-3 expression by 62.1%, the extract from Comparative Example 3 by 78.4%, the mixture from Comparative Example 4 by 27.2%, the mixture from Comparative Example 5 by 61.5%, and the mixture from Comparative Example 6 by 59.8%. Furthermore, no significant increase in AQP-3 expression was observed in the extract from Comparative Example 1. Based on these results, it can be confirmed that the mixture from Example 1 is most effective for cell hydration. Figure 2 ). Figure 2 The results of the cell hydration effect were confirmed by adjusting the expression of aquaporin-3 by the extracts of Comparative Examples 1 to 3 or the mixture of Comparative Examples 4 to 6 and Example 1.

[0070] Experiment Example 3: Confirming the intercellular hydration effect by confirming the expression of claudin-1

[0071] The intercellular hydration effect of extracts of Comparative Examples 1 to 3 or mixtures of Comparative Examples 4 to 6 and Example 1 on Claudin-1, which is associated with moisture loss in the skin, was determined by the following method.

[0072] When HaCaT cells were aliquoted into 6-well plates and filled to 80%, extracts from Comparative Examples 1 to 3, or a mixture of Comparative Examples 4 to 6 and Example 1, were treated with culture medium at a concentration of 100 μg / ml. Cells were then cultured for 24 hours and collected. Proteins were then separated using 1× cell lysis buffer. Proteins were quantified using the BCA assay, and a sample was electrophoresed on a 10% SDS-PAGE gel. The protein was then transferred to a PVDF membrane and blocked with 5% skim milk for 1 hour. Primary antibody (Claudin-1, Cell Signaling, USA) was incubated overnight at 4°C, followed by HRP-conjugated secondary antibody incubation at room temperature for 2 hours. The cells were then washed with TBS / T and the bands were confirmed using an ECL reaction kit via a Chemi-Doc instrument. The quantification of each band was based on the expression of β-actin, thereby calculating the expression level of Claudin-1.

[0073] Experimental results showed that the mixture of Example 1 increased the expression of Claudin-1 by approximately 112.5% ​​at a concentration of 100 μg / ml. The extract of Comparative Example 1 increased the expression of Claudin-1 by 62.2%, the extract of Comparative Example 2 by 13.4%, the mixture of Comparative Example 4 by 61.3%, the mixture of Comparative Example 5 by 67.8%, and the mixture of Comparative Example 6 by 22.9%. In the case of the extract of Comparative Example 3, there was no significant increase in the expression of Claudin-1. Therefore, it can be confirmed that the mixture of Example 1 is most effective for intercellular hydration of the skin. Figure 3 ). Figure 3 The results of the intercellular hydration effect were confirmed by adjusting the expression of Claudin-1 by the extracts of Comparative Examples 1 to 3 or the mixture of Comparative Examples 4 to 6 and Example 1.

[0074] Experiment Example 4: Confirming Water Holding Ability

[0075] In this experiment, the water holding capacity of the extracts of Comparative Examples 1 to 3, or the mixture of Comparative Examples 4 to 6 and Example 1 was measured. A solution of water with added hyaluronic acid (HA) was used as a comparative sample.

[0076] Therefore, experiments were conducted at a constant temperature. 20 ml of each of the extracts of Comparative Examples 1 to 3 or the mixture of Comparative Examples 4 to 6 and Example 1 was placed in a petri dish, and the weight was measured by a precision balance at different times (0 hours, 2 hours, 4 hours, 6 hours, 12 hours and 24 hours) and recorded in Table 2 below.

[0077] Table 2

[0078]

[0079] The experimental results showed that the amount of moisture reduction in Example 1 (21.9%), Comparative Example 6 (27.0%), Comparative Example 4 (28.8%), Comparative Example 5 (30.2%), Comparative Example 2 (30.6%), Comparative Example 1 (34.3%), Comparative Example 3 (34.8%), and Water (HA) (39.0%) decreased sequentially.

[0080] That is, it can be confirmed that the mixture of Example 1 has superior binding force with water and the least amount of water evaporated compared with the extracts of Comparative Examples 1 to 3 or the mixtures of Comparative Examples 4 to 6. Figure 4 ). Figure 4 To show the results of moisture reduction rates of the extracts of Comparative Examples 1 to 3 or the mixtures of Comparative Examples 4 to 6 and Example 1.

[0081] Dosage Form Example 1: Preparation of Cosmetic Compositions

[0082] As a mixture of Example 1 above, a cosmetic composition containing 0.1% by weight of hydrolyzed malt extract, hydrolyzed gardenia Florida extract and hydrolyzed viola tricolor extract was prepared according to the composition shown in Table 3 below and referred to as dosage form example 1. Comparative dosage form example 1 was prepared as a control group.

[0083] Table 3

[0084]

[0085] Experiment Example 5: Determination of Skin Moisture Content

[0086] In this experiment, the skin moisture content of the dosage form Example 1 was confirmed. Therefore, skin hydration capacity was evaluated by measuring skin moisture content. The hydration capacity was measured using a skin moisture meter via capacitance measurement. This method measures the moisture content of the skin surface (e.g., the stratum corneum). The advantage of this method is that it is unaffected by samples already applied locally when measuring skin surface moisture content, and maintains a constant measurement level at a depth of 30 μm to 40 μm below the probe sensor contact area. The measurement unit is displayed in the arbitrary unit (AU) specified by the device.

[0087] Furthermore, under constant temperature and humidity conditions of 20℃~25℃ and relative humidity of 40%~55%, the initial values ​​of the above-mentioned dosage form Example 1 and comparative dosage form Example 1 were applied after measuring the initial values ​​of the skin moisture content before application using a skin moisture meter (Corneometer). Then, the skin moisture content was measured using a skin moisture meter at different times (10 minutes, 30 minutes, 60 minutes, 90 minutes and 180 minutes), and the results are shown in Table 4 below.

[0088] Table 4

[0089] (Unit: AU) 0 minutes 10 minutes 30 minutes 60 minutes 90 minutes 180 minutes Dosage Form Example 1 35.5 46.5 45.8 43.5 40.4 38.1 Comparative Dosage Form Example 1 35.9 40.7 38.4 36.4 35.4 36.1

[0090] The experimental results confirmed that, compared with the control group, dosage form 1 showed a significant increase in skin moisture content. Figure 5 ). Figure 5 The results were determined when the skin moisture content was measured using dosage form 1 and comparative dosage form 1.

[0091] Experimental Example 6: Measurement of transepidermal water loss

[0092] In this experiment, the transepidermal water loss (TEWL) of the above-mentioned dosage form Example 1 was confirmed. Transepidermal water loss refers to the evaporation of water from the skin when the skin barrier function is impaired. Water evaporation was evaluated in g / h / m² per hour. 2 express.

[0093] After manually removing the keratin from the skin by binding the skin on the inner side of the lower arm with tape, the above-mentioned dosage form Example 1 and comparative dosage form Example 1 were applied. Then, the skin moisture evaporation was measured at different times (10 minutes, 30 minutes, 60 minutes, 90 minutes and 180 minutes) using a transdermal moisture loss meter (Tewameter TM 210, Courage and Khazaka, Germany), and the results are shown in Table 5 below.

[0094] Table 5

[0095] <![CDATA[(Unit: g / m 2 / h)]]> 0 minutes 10 minutes 30 minutes 60 minutes 90 minutes 180 minutes Dosage Form Example 1 10.4 20.7 12.4 11.1 10.8 10.1 Comparative Dosage Form Example 1 10.9 21.4 18.4 16.5 13.4 12.1

[0096] The experimental results confirm that, compared with the comparative dosage form 1, dosage form 1 showed a very low transdermal water loss. Figure 6 ). Figure 6 The results were used to determine the amount of transdermal water loss when using dosage form 1 and comparative dosage form 1.

Claims

1. A cosmetic composition for skin hydration, characterized in that, It contains equal proportions of hydrolyzed malt extract, hydrolyzed gardenia extract, and hydrolyzed pansy extract. The hydrolyzed malt extract, hydrolyzed gardenia extract, and hydrolyzed pansy extract were prepared by hot water extraction of malt, gardenia, and pansy at 100°C, followed by hydrolysis and neutralization with HCl solution at 25°C.

2. The skin moisturizing cosmetic composition according to claim 1, characterized in that, The above-mentioned hydration includes any one or more of cellular hydration and intercellular hydration.

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