A reagent delivery fluid system

Abstract

The present disclosure provides a reagent delivery fluid system, comprising: a reagent kit, a waste liquid box and a reagent reaction device; the reagent reaction device comprises a flow cell and a syringe pump; the waste liquid box and the reagent kit are respectively connected to the flow cell; the flow cell is connected to the syringe pump; the reagent kit is used to store reagents; the waste liquid box is used to store waste liquid; the flow cell is used to extract the reagent from the reagent kit when the piston of the syringe pump moves from the inside to the outside, and perform a reagent reaction based on the reagent to generate a reagent reaction liquid; the reagent reaction liquid is input into the syringe pump; the syringe pump is used to control the piston to move from the outside to the inside to input the reagent reaction liquid into the flow cell; the flow cell is used to perform a reagent reaction based on the reagent reaction liquid to generate waste liquid, and the waste liquid is input into the waste liquid box.

Description

Technical Field

[0001] The present disclosure relates to the field of gene detection technology, and in particular to a reagent delivery fluid system. Background Art

[0002] To ensure a full reaction, gene sequencing systems typically recirculate reagents flowing through a flow cell back into the flow cell, allowing the reagents to react multiple times within the flow cell. This process typically reduces the active ingredient content of the reagents recirculated back into the flow cell. These reagents must then be recirculated through the flow cell before being discharged. However, this process introduces impurities into the flow cell, resulting in lower sequencing accuracy. Summary of the Invention

[0003] In view of this, the present disclosure at least provides a reagent delivery fluid system.

[0004] In a first aspect, the present disclosure provides a reagent delivery fluid system, comprising: a reagent reagent box, a waste liquid box, and a reagent reaction device; the reagent reaction device comprises a flow cell and a syringe pump; the waste liquid box and the reagent reagent box are respectively connected to the flow cell; the flow cell is connected to the syringe pump;

[0005] The reagent box is used to store reagents; the waste liquid box is used to store waste liquid;

[0006] The flow cell is used to extract the reagent from the reagent kit when the piston of the syringe pump moves from the inside to the outside, and to perform a reagent reaction based on the reagent to generate a reagent reaction liquid; and to input the reagent reaction liquid into the syringe pump;

[0007] The syringe pump is used to control the piston to move from outside to inside to input the reagent reaction solution into the flow cell;

[0008] The flow cell is used to perform a reagent reaction based on the reagent reaction solution, generate waste liquid, and input the waste liquid into the waste liquid box.

[0009] In the embodiment of the present disclosure, by connecting the reagent kit with the flow pool, the flow pool can extract the reagent from the reagent kit when the piston of the injection pump moves from the inside to the outside, and perform a reagent reaction based on the reagent to generate a reagent reaction liquid; the reagent reaction liquid is input into the injection pump; at the same time, by connecting the waste liquid box with the flow pool, the flow pool can directly input the waste liquid into the waste liquid box after the reagent reaction is performed based on the reagent reaction liquid to generate waste liquid when the injection pump inputs the reagent reaction liquid with low effective ingredients into the flow pool, without discharging the waste liquid through the flow pool, thereby avoiding bringing more impurities into the flow pool, improving the efficiency of reagent delivery fluid, and thereby improving the accuracy of sequencing.

[0010] In a possible embodiment, the reagent reaction device further includes: a first connecting valve; the first connecting valve is connected to the waste liquid box, the reagent kit and the flow cell respectively;

[0011] The flow pool is used to extract the reagent from the reagent kit through the first connecting valve when extracting the reagent from the reagent kit; and to input the waste liquid into the waste liquid box through the first connecting valve when inputting the waste liquid into the waste liquid box.

[0012] Here, the reagent box and the waste liquid box are connected to the flow pool respectively through the first connecting valve, so that the flow pool can extract reagents from the reagent box and input waste liquid into the waste liquid box, which can avoid confusion between reagents and waste liquid and improve the efficiency of the reagent delivery fluid system.

[0013] In a possible embodiment, the reagent reaction device further includes: a second connecting valve, the second connecting valve being connected to the flow cell and the injection pump respectively;

[0014] The flow cell is used to: input the reagent reaction solution into the syringe pump through the second connecting valve when the reagent reaction solution is input into the syringe pump;

[0015] When inputting the reagent reaction solution into the flow cell, the injection pump is used to: input the reagent reaction solution into the flow cell through the second connecting valve.

[0016] Considering that when the flow pool is directly connected to the injection pump, the flow pool will input waste liquid into the waste liquid box due to misoperation of the injection pump, connecting the flow pool to the injection pump through the second connecting valve can better control the flow of reagents and improve the efficiency of the reagent delivery fluid system.

[0017] In one possible embodiment, there are multiple flow cells, multiple second connecting valves, and multiple injection pumps; and the number of the second connecting valves and the number of the injection pumps are respectively consistent with the number of the flow cells; and each second connecting valve is respectively connected to one flow cell and one injection pump.

[0018] In order to reduce the experimental time and speed up the experiment, multiple flow cells, second connecting valves and injection pumps are set to work simultaneously, which can increase the flow volume of the flow cell, improve the efficiency of the reagent delivery fluid system, and thus improve the experimental efficiency.

[0019] In one possible embodiment, the reagent reaction device includes multiple groups of circulation modules, each group of circulation modules includes multiple flow cells; the number of the first connecting valves is consistent with the number of groups of the circulation modules, the number of the second connecting valves is consistent with the number of flow cells included in each group of circulation modules; the number of the syringe pumps is consistent with the number of the second connecting valves;

[0020] The plurality of flow cells in each group of flow modules are respectively connected to one of the first connecting valves;

[0021] Each of the second connecting valves is connected to a flow cell in each group of the flow modules;

[0022] Each of the second connecting valves is connected to one of the injection pumps.

[0023] Here, by providing multiple groups of circulation modules, each group of circulation modules can work alternately, thereby reducing the waiting time of the circulation modules, improving the reagent reaction efficiency, and further improving the efficiency of the reagent delivery fluid system.

[0024] In a possible embodiment, the system includes: a plurality of reagent kits, wherein the plurality of reagent kits are used to store different reagents; the plurality of waste liquid boxes includes a plurality of waste liquid boxes, and each reagent kit corresponds to a waste liquid box.

[0025] Taking into account the different quantities of reagent types required for different reagent reactions, the reagent delivery fluid system can include multiple reagent kits that can store different reagents to meet more experimental needs; at the same time, each reagent kit corresponds to a waste liquid box, and each waste liquid box can store one type of waste liquid, which can avoid the reaction of multiple waste liquids and ensure the safety of waste liquid treatment.

[0026] In a possible embodiment, the system further includes: a pipeline, through which the waste liquid box and the reagent kit are respectively connected to the flow cell; and the flow cell is connected to the injection pump through the pipeline.

[0027] In order to make the above-mentioned objectives, features and advantages of the present disclosure more obvious and easy to understand, preferred embodiments are given below and described in detail with reference to the accompanying drawings. BRIEF DESCRIPTION OF THE DRAWINGS

[0028] In order to more clearly illustrate the technical solutions of the embodiments of the present disclosure, the following briefly introduces the drawings required for use in the embodiments. The drawings herein are incorporated into and constitute a part of the specification. These drawings illustrate embodiments consistent with the present disclosure and, together with the specification, are used to illustrate the technical solutions of the present disclosure. It should be understood that the following drawings only illustrate certain embodiments of the present disclosure and should not be regarded as limiting the scope. For those of ordinary skill in the art, other relevant drawings can be obtained based on these drawings without inventive effort.

[0029] Figure 1 A schematic diagram of the structure of a reagent delivery fluid system provided by an embodiment of the present disclosure is shown;

[0030] Figure 2 A schematic diagram of the architecture of another reagent delivery fluid system provided by an embodiment of the present disclosure is shown;

[0031] Figure 3 A schematic diagram of the architecture of another reagent delivery fluid system provided by an embodiment of the present disclosure is shown;

[0032] Figure 4 A schematic diagram of the architecture of another reagent delivery fluid system provided by an embodiment of the present disclosure is shown;

[0033] Figure 5 A schematic diagram of the architecture of another reagent delivery fluid system provided by an embodiment of the present disclosure is shown;

[0034] Figure 6 A schematic diagram of the architecture of another reagent delivery fluid system provided by an embodiment of the present disclosure is shown. DETAILED DESCRIPTION

[0035] In order to make the purpose, technical solutions and advantages of the embodiments of the present disclosure clearer, the technical solutions in the embodiments of the present disclosure will be clearly and completely described below in conjunction with the drawings in the embodiments of the present disclosure. Obviously, the described embodiments are only part of the embodiments of the present disclosure, not all of the embodiments. The components of the embodiments of the present disclosure generally described and shown in the drawings herein can be arranged and designed in various different configurations. Therefore, the following detailed description of the embodiments of the present disclosure provided in the drawings is not intended to limit the scope of the present disclosure for protection, but merely represents selected embodiments of the present disclosure. Based on the embodiments of the present disclosure, all other embodiments obtained by those skilled in the art without making creative work are within the scope of protection of the present disclosure.

[0036] Generally, when using a gene sequencing system to detect a genome, in order to allow the reagents to fully react, the reagents flowing through the flow cell will be pushed back into the flow cell, allowing the reagents to react multiple times in the flow cell. Usually, a temporary storage box is set between the reagent kit and the flow cell to store waste liquid; the specific process is as follows: first, the reagent is transported to the flow cell, and a reagent reaction is carried out based on the reagent to generate a reagent reaction liquid; then the waste liquid is transported to the injection pump. Then, the reagent reaction liquid is transported to the flow cell, and a reagent reaction is carried out based on the reagent reaction liquid to generate waste liquid, and then the waste liquid is transported to the temporary storage box.

[0037] Because the active ingredients in the reagent reaction solution have been reduced, the waste liquid urgently needs to be discharged from the device. However, in the related art, the waste liquid must first be transported to a flow cell and then discharged from a syringe pump. During this process, the waste liquid can introduce impurities into the flow cell, resulting in lower gene sequencing accuracy. To alleviate this problem, the present disclosure proposes a reagent delivery fluid system.

[0038] It should be noted that similar reference numerals and letters denote similar items in the following drawings, and therefore, once an item is defined in one drawing, it does not require further definition or explanation in subsequent drawings.

[0039] See also Figure 1 As shown, it is a schematic diagram of the architecture of the reagent delivery fluid system provided in an embodiment of the present disclosure, including: a reagent reagent box 11, a waste liquid box 12 and a reagent reaction device 13; the reagent reaction device 13 includes a flow pool 131 and an injection pump 132; the waste liquid box 12 and the reagent reagent 11 are respectively connected to the flow pool 131; the flow pool 131 is connected to the injection pump 132.

[0040] The reagent box 11 is used for storing reagents; the waste liquid box 12 is used for storing waste liquid.

[0041] The flow cell 131 is used to extract the reagent from the reagent kit when the piston of the syringe pump moves from the inside to the outside, perform a reagent reaction based on the reagent, generate a reagent reaction liquid, and input the reagent reaction liquid into the syringe pump.

[0042] The syringe pump 132 is used to control the piston to move from outside to inside to input the reagent reaction solution into the flow cell.

[0043] The flow cell 131 is used to perform a reagent reaction based on the reagent reaction solution, generate a waste liquid, and input the waste liquid into a waste liquid box.

[0044] During implementation, the reagent delivery fluid system may include a reagent reagent box, a waste liquid box and a reagent reaction device, and the reagent reaction device may include a flow cell and an injection pump; illustratively, the waste liquid box and the reagent reagent may be respectively connected to the flow cell, and the flow cell may be connected to the injection pump.

[0045] In specific implementations, the test kit can be used to store reagents, and the waste liquid box can be used to store waste liquid. First, the syringe pump can control the piston to move from the inside to the outside, that is, to control the piston to move away from the flow cell; the flow cell can extract reagents from the test kit and perform a reagent reaction based on the reagent to generate a reagent reaction liquid, that is, used reagent; further, the waste liquid can be input into the syringe pump. Then, the syringe pump can control the piston to move from the outside to the inside, that is, to control the piston to move toward the flow cell, to input the reagent reaction liquid into the flow cell; the flow cell can perform a reagent reaction based on the reagent reaction liquid to generate waste liquid, and the waste liquid can be input into the waste liquid box.

[0046] In the embodiment of the present disclosure, by connecting the reagent kit with the flow pool, the flow pool can extract the reagent from the reagent kit when the piston of the injection pump moves from the inside to the outside, and perform a reagent reaction based on the reagent to generate a reagent reaction liquid; the reagent reaction liquid is input into the injection pump; at the same time, by connecting the waste liquid box with the flow pool, the flow pool can directly input the waste liquid into the waste liquid box after the reagent reaction is performed based on the reagent reaction liquid to generate waste liquid when the injection pump inputs the reagent reaction liquid with low effective ingredients into the flow pool, without discharging the waste liquid through the flow pool, thereby avoiding bringing more impurities into the flow pool, improving the efficiency of reagent delivery fluid, and thereby improving the accuracy of sequencing.

[0047] In one possible implementation, see Figure 2 As shown, the reagent reaction device 13 further includes: a first connecting valve 133; the first connecting valve 133 is connected to the waste liquid box 12, the reagent kit 11 and the flow cell 131 respectively.

[0048] The flow cell 131 is used to extract reagents from the reagent box through the first connecting valve when extracting reagents from the reagent box; and to input waste liquid into the waste liquid box through the first connecting valve when inputting waste liquid into the waste liquid box.

[0049] During implementation, the reagent reaction device may further include a first connecting valve, which may be connected to the waste liquid box, the reagent kit, and the flow cell, respectively. The first connecting valve may be configured according to actual conditions, for example, a two-connection valve, a four-connection valve, a twenty-four-connection valve, and the like.

[0050] Illustratively, if the first interconnecting valve is a twenty-four-connecting valve, i.e., it includes 24 branches and 1 common passage, the reagent kit can be connected to any of the 24 branches included in the first interconnecting valve, for example, it can be connected to the first branch of the first interconnecting valve; the waste liquid box can be connected to any of the 24 branches included in the first interconnecting valve except the first branch, for example, it can be connected to the 24th branch of the first interconnecting valve; the flow pool can be connected to the common passage of the first interconnecting valve.

[0051] In specific implementation, the above example will be continued for explanation. First, the first connecting valve can control the valve core to rotate to the branch connected to the reagent kit, that is, the first branch; at this time, the injection pump can control the piston to move from the inside to the outside, that is, to control the piston to move in the direction away from the flow pool; the flow pool can extract reagents from the reagent kit through the first connecting valve, and can perform a reagent reaction based on the reagent to generate a reagent reaction liquid; further, the waste liquid can be input into the injection pump. Then, the first connecting valve can control the valve core to rotate to the branch connected to the waste liquid box, that is, the 24th branch; the injection pump can control the piston to move from the outside to the inside, that is, to control the piston to move in the direction close to the flow pool, and input the reagent reaction liquid into the flow pool; the flow pool can perform a reagent reaction based on the reagent reaction liquid to generate waste liquid, and the waste liquid can be input into the waste liquid box through the first connecting valve.

[0052] Here, the reagent box and the waste liquid box are connected to the flow pool respectively through the first connecting valve, so that the flow pool can extract reagents from the reagent box and input waste liquid into the waste liquid box, which can avoid confusion between reagents and waste liquid and improve the efficiency of the reagent delivery fluid system.

[0053] In one possible implementation, see Figure 2 As shown, the reagent reaction device 13 further includes: a second connecting valve 134, and the second connecting valve 134 is connected to the flow cell 131 and the injection pump 132 respectively.

[0054] The flow cell 131 is used to input the reagent reaction solution into the syringe pump through the second connecting valve when the reagent reaction solution is input into the syringe pump.

[0055] The syringe pump 132 is used to input the reagent reaction solution into the flow cell through the second connecting valve 5 when inputting the reagent reaction solution into the flow cell.

[0056] During implementation, the reagent reaction device may further include a second connecting valve, which may be connected to the flow cell and the injection pump, respectively. The second connecting valve may be configured according to actual conditions, for example, a two-connection valve, a four-connection valve, a six-connection valve, and the like.

[0057] For example, if the second connecting valve is a six-way valve, that is, it includes six branches and one common channel, the flow cell can be connected to any of the six branches included in the second connecting valve, for example,

[0058] It can be connected to the third branch of the second connecting valve; the injection pump can be connected to the common passage of the second connecting valve.

[0059] In a specific implementation, continuing with the above example, the second connecting valve can first control the valve core to rotate to the branch connected to the flow cell, namely the third branch. At this time, the syringe pump can control the fifth piston to move from the inside to the outside, that is, to control the piston to move away from the flow cell. The flow cell can then input the reagent reaction solution into the syringe pump through the second connecting valve. Then, the syringe pump can control the piston to move from the outside to the inside, that is, to control the piston to move toward the flow cell, and input the reagent reaction solution into the flow cell through the second connecting valve.

[0060] Considering that when the flow pool is directly connected to the injection pump, the flow pool will input waste liquid into the waste liquid box due to misoperation of the injection pump, connecting the flow pool to the injection pump through the second connecting valve can better control the flow of reagents and improve the efficiency of the reagent delivery fluid system.

[0061] In one possible implementation, see Figure 3 As shown, the number of the flow cell 131, the second communication valve 134 and the injection pump 132 is multiple; and the number of the second communication valve 134, the number of the injection pump 132

[0062] The amounts are consistent with the number of flow cells 131; each second connecting valve 134 is connected to a flow cell 131 and a syringe pump 132 respectively.

[0063] During implementation, the reagent reaction device may further include multiple flow cells, second connecting valves and injection pumps; wherein the number of second connecting valves and the number of injection pumps are consistent with the number of flow cells, and each second connecting valve is connected to a flow cell and a injection pump respectively.

[0064] For example, see Figure 3 As shown, if the reagent reaction device includes a second connecting valve A, a second connecting valve B, a syringe pump A, a syringe pump B, and a flow cell A, a flow cell B, then the second connecting valve A can be connected to the syringe pump A and the flow cell A respectively, and the second connecting valve B can be connected to the syringe pump B and the flow cell B respectively; the flow cell A can be connected to the reagent kit and the waste liquid box respectively, and the flow cell B can be connected to the reagent kit and the waste liquid box respectively. If the reagent reaction device also includes a first connecting valve, then the flow cell A and the flow cell B can be connected to the first connecting valve respectively.

[0065] In specific implementation, the above example will be used for further explanation. First, the first interconnecting valve can control the valve core to rotate to the branch connected to the reagent cartridge. At this point, syringe pumps A and B can simultaneously control the pistons to move from the inside out. Flow cell A can extract reagents from the reagent cartridge via the first interconnecting valve and perform a reagent reaction based on the reagents to generate a reagent reaction solution. Wastewater can be input to syringe pump A via the second interconnecting valve A. Simultaneously, flow cell B can extract reagents from the reagent cartridge via the first interconnecting valve and perform a reagent reaction based on the reagents to generate a reagent reaction solution. Wastewater can be input to syringe pump B via the second interconnecting valve B.

[0066] Then, the first connecting valve can control the valve core to rotate to the branch connected to the waste liquid box; the injection pump A and the injection pump B can simultaneously control the piston to move from outside to inside; the injection pump A can input the reagent reaction liquid into the flow pool A through the second connecting valve A, and at the same time, the injection pump B can input the reagent reaction liquid into the flow pool B through the second connecting valve B; the flow pool A and the flow pool B can simultaneously perform reagent reactions based on the reagent reaction liquid to generate waste liquid, and can input the waste liquid into the waste liquid box through the first connecting valve.

[0067] In order to reduce the experimental time and speed up the experiment, multiple flow cells, second connecting valves and injection pumps are set to work simultaneously, which can increase the flow volume of the flow cell, improve the efficiency of the reagent delivery fluid system, and thus improve the experimental efficiency.

[0068] In one possible implementation, see Figure 4 As shown, the reagent reaction device 13 includes multiple groups of circulation modules, each group of circulation modules includes multiple flow pools; the number of first connecting valves is consistent with the number of groups of circulation modules, and the number of second connecting valves is consistent with the number of flow pools included in each group of circulation modules; the number of injection pumps is consistent with the number of second connecting valves.

[0069] The multiple flow cells in each group of circulation modules are respectively connected to a first connecting valve; each second connecting valve is connected to a flow cell in each group of circulation modules; and each second connecting valve is connected to an injection pump.

[0070] During implementation, the reagent reaction device may include multiple groups of circulation modules, each group of circulation modules may include multiple flow cells; the number of first connecting valves is consistent with the number of groups of circulation modules, the number of second connecting valves is consistent with the number of flow cells included in each group of circulation modules; and the number of syringe pumps is consistent with the number of second connecting valves. The multiple flow cells in each group of circulation modules may be respectively connected to a first connecting valve; each second connecting valve may be connected to a flow cell in each group of circulation modules; and each second connecting valve may be connected to a syringe pump.

[0071] For example, see Figure 4As shown, the reagent reaction device 13 includes two groups of circulation modules, namely, circulation module 1 and circulation module 2, and each group of circulation modules includes two flow cells, namely, circulation module 1 includes flow cell A and flow cell C, and circulation module 2 includes flow cell B and flow cell D. In this case, the reagent reaction device also includes a plurality of first connecting valves, a plurality of second connecting valves, and a plurality of injection pumps; and the number of first connecting valves is consistent with the number of groups of circulation modules, namely, it includes two first connecting valves, first connecting valve A and first connecting valve B; the number of second connecting valves is consistent with the number of flow cells included in each group of circulation modules, namely, it includes two second connecting valves, second connecting valve A and second connecting valve B; and the number of injection pumps is consistent with the number of second connecting valves, namely, it includes two injection pumps, injection pump A and injection pump B.

[0072] The plurality of flow cells in each group of flow modules can be respectively connected to a first connecting valve, that is, each group of flow modules corresponds to a first connecting valve, for example, Figure 4 As shown, the first connecting valve A is connected to the flow pool A and the flow pool C included in the circulation module 1, and the first connecting valve B is connected to the flow pool B and the flow pool D included in the circulation module 2; each second connecting valve can be connected to a flow pool in each group of circulation modules, for example, Figure 4 As shown, the second connecting valve A is connected to the flow pool A in the circulation module 1 and the flow pool B in the circulation module 2, and the second connecting valve B is connected to the flow pool C in the circulation module 1 and the flow pool D in the circulation module 2; each second connecting valve can be connected to a syringe pump, for example, Figure 4 As shown, the second connecting valve A is connected to the injection pump A, and the second connecting valve B is connected to the injection pump B.

[0073] In specific implementation, the multiple groups of flow modules included in the reagent reaction device can work alternately, and the multiple flow cells in each group of flow modules can work simultaneously. Figure 4 As shown, first, the first connecting valve A can control the valve core to rotate to the branch connected to the reagent kit, the second connecting valve A can control the valve core to rotate to the branch connected to the flow pool A, and the second connecting valve B can control the valve core to rotate to the branch connected to the flow pool C; at this time, the injection pump A and the injection pump B can simultaneously control the piston to move from the inside to the outside; the flow pool A in the circulation module 1 can extract reagents from the reagent kit through the first connecting valve A, and can perform reagent reactions based on the reagents to generate reagent reaction liquid; the waste liquid can be input to the injection pump A through the second connecting valve A; and the flow pool C can extract reagents from the reagent kit through the first connecting valve A, and can perform reagent reactions based on the reagents to generate reagent reaction liquid; the waste liquid can be input to the injection pump B through the second connecting valve B.

[0074] Then, first interconnecting valve A can control the valve core to rotate to the branch connected to the waste liquid box; syringe pump A and syringe pump B can simultaneously control the piston to move from outside to inside; syringe pump A can input the reagent reaction liquid into flow cell A through second interconnecting valve A, and syringe pump B can input the reagent reaction liquid into flow cell C through second interconnecting valve B; flow cell A and flow cell C can simultaneously perform reagent reactions based on the reagent reaction liquid, generate waste liquid, and input the waste liquid into the waste liquid box through first interconnecting valve A. At this point, a test result can be generated based on the reagent reaction results of flow cell A and flow cell C.

[0075] During specific implementation, while generating detection results based on the reagent reaction results of flow pool A and flow pool C, the circulation module 2 can be controlled to work; first, the first connecting valve B can control the valve core to rotate to the branch connected to the test kit, the second connecting valve A can control the valve core to rotate to the branch connected to the flow pool B, and the second connecting valve B can control the valve core to rotate to the branch connected to the flow pool D; at this time, the injection pump A and the injection pump B can simultaneously control the piston to move from the inside to the outside; the flow pool B in the circulation module 2 can extract reagents from the test kit through the first connecting valve B, and can perform reagent reactions based on the reagents to generate reagent reaction liquid; the waste liquid can be input to the injection pump A through the second connecting valve A; and the flow pool D can extract reagents from the test kit through the first connecting valve B, and can perform reagent reactions based on the reagents to generate reagent reaction liquid; the waste liquid can be input to the injection pump B through the second connecting valve B.

[0076] Then, first interconnecting valve B can control the valve core to rotate to the branch connected to the waste liquid box; syringe pump A and syringe pump B can simultaneously control the piston to move from outside to inside; syringe pump A can input the reagent reaction liquid into flow cell B through second interconnecting valve A, and syringe pump B can input the reagent reaction liquid into flow cell D through second interconnecting valve B; flow cell B and flow cell D can simultaneously perform reagent reactions based on the reagent reaction liquid, generate waste liquid, and input the waste liquid into the waste liquid box through first interconnecting valve B. At this time, a test result can be generated based on the reagent reaction results of flow cell B and flow cell D.

[0077] In a specific implementation, while generating the detection results based on the reagent reaction results of the flow pool B and the flow pool D, the circulation module 1 can be controlled to work, so that multiple circulation modules can work alternately.

[0078] Here, by providing multiple groups of circulation modules, each group of circulation modules can work alternately, thereby reducing the waiting time of the circulation modules, improving the reagent reaction efficiency, and further improving the efficiency of the reagent delivery fluid system.

[0079] In one possible implementation, see Figure 5As shown, the system includes: a plurality of reagent kits 11 for storing different reagents, and a plurality of waste liquid boxes 12, with each reagent kit corresponding to a waste liquid box.

[0080] During implementation, considering that there may be multiple types of reagents required in the reagent reaction process, the reagent delivery fluid system may also include multiple test kits, and the multiple test kits can be used to store different reagents. For example, two test kits are included, test kit A can be used to store reagent A, and test kit B can be used to store reagent B; in the case of including multiple test kits, the waste liquid box may also include multiple, and each test kit corresponds to a waste liquid box, for example, test kit A can correspond to waste liquid box A, and test kit B can correspond to waste liquid box B.

[0081] For example, see Figure 5 As shown, in the case where the reagent kit A, the reagent kit B, the waste liquid box A, and the waste liquid box B are included, and the reagent kit A corresponds to the waste liquid box A, and the reagent kit B corresponds to the waste liquid box B, if the first connecting valve is a twenty-four-connecting valve, the reagent kit A can be connected to the first branch of the first connecting valve, and the waste liquid box A can be connected to the second branch of the first connecting valve; the reagent kit B can be connected to the third branch of the first connecting valve, and the waste liquid box B can be connected to the fourth branch of the first connecting valve.

[0082] If reagents are delivered in the order of reagent kit A and reagent kit B, first, the first connecting valve can control the valve core to rotate to the branch connected to reagent kit A, and the syringe pump can control the piston to move from the inside to the outside. The flow cell can extract reagents from reagent kit A through the first connecting valve and perform a reagent reaction based on the reagents to generate reagent reaction liquid A. Then, waste liquid A can be input into the syringe pump through the second connecting valve. Then, the first connecting valve can control the valve core to rotate to the branch connected to waste liquid box A, and the syringe pump can control the piston to move from the outside to the inside, inputting reagent reaction liquid A into the flow cell through the second connecting valve. The flow cell can perform a reagent reaction based on reagent reaction liquid A to generate waste liquid A, and waste liquid A can be input into waste liquid box A through the first connecting valve.

[0083] Then, the first connecting valve can control the valve core to rotate to the branch connected to the reagent cartridge B, and the syringe pump can control the piston to move from the inside to the outside. The flow cell can extract the reagent from the reagent cartridge B through the first connecting valve, and can perform a reagent reaction based on the reagent to generate a reagent reaction solution B. The waste solution B can then be input into the syringe pump through the second connecting valve. Then, the first connecting valve can control the valve core to rotate to the branch connected to the waste liquid cartridge B. The syringe pump can control the piston to move from the outside to the inside, and input the reagent reaction solution B into the flow cell through the second connecting valve. The flow cell can perform a reagent reaction based on the reagent reaction solution B to generate a waste solution B, and the waste solution B can then be input into the waste liquid cartridge B through the first connecting valve.

[0084] Taking into account the different quantities of reagent types required for different reagent reactions, the reagent delivery fluid system can include multiple reagent kits that can store different reagents to meet more experimental needs; at the same time, each reagent kit corresponds to a waste liquid box, and each waste liquid box can store one type of waste liquid, which can avoid the reaction of multiple waste liquids and ensure the safety of waste liquid treatment.

[0085] In one possible implementation, see Figure 6 As shown, the system further includes: a pipeline 14 , through which the waste liquid box 12 and the reagent kit 11 are respectively connected to the flow cell 131 ; and the flow cell 131 is connected to the injection pump 132 through the pipeline 14 .

[0086] During implementation, the reagent delivery fluid system may further include a pipeline, wherein the waste liquid box and the reagent reagent box may be connected to the flow cell through the pipeline respectively; the flow cell and the syringe pump may be connected through the pipeline. The pipeline may be used to deliver at least one of the reagent, the reagent reaction solution, and the waste liquid.

[0087] In the several embodiments provided in the present disclosure, it should be understood that the disclosed system can be implemented in other ways. The system embodiments described above are merely illustrative. For example, the division of the units is merely a logical function division. There may be other division methods in actual implementation. For example, multiple units or components may be combined or integrated into another system, or some features may be ignored or not executed. Another point is that the mutual coupling or direct coupling or communication connection shown or discussed may be an indirect coupling or communication connection through some communication interface, device or unit, which may be electrical, mechanical or other forms.

[0088] The units described as separate components may or may not be physically separate, and the components shown as units may or may not be physical units, that is, they may be located in one place or distributed across multiple network units. Some or all of these units may be selected to achieve the purpose of this embodiment according to actual needs.

[0089] In addition, each functional unit in each embodiment of the present disclosure may be integrated into one processing unit, or each unit may exist physically separately, or two or more units may be integrated into one unit.

[0090] The above are only specific embodiments of the present disclosure, but the scope of protection of the present disclosure is not limited thereto. Any changes or substitutions that can be easily conceived by a person skilled in the art within the technical scope disclosed in this disclosure should be included in the scope of protection of the present disclosure. Therefore, the scope of protection of the present disclosure should be based on the scope of protection of the claims.

Claims

1. A reagent delivery fluid system, characterized in that: include: Reagent kits, waste liquid boxes and reagent reaction devices; The reagent reaction device includes a flow cell and a syringe pump; the waste liquid box and the reagent kit are respectively connected to the first end of the flow cell; the syringe pump is connected to the second end of the flow cell; The reagent box is used to store reagents; the waste liquid box is used to store waste liquid; The flow cell is used to extract the reagent from the reagent kit when the piston of the syringe pump moves from the inside to the outside, and to perform a reagent reaction based on the reagent to generate a reagent reaction liquid; and to input the reagent reaction liquid into the syringe pump; The syringe pump is used to control the piston to move from outside to inside to input the reagent reaction solution into the flow cell; The flow cell is used to perform a reagent reaction based on the reagent reaction solution, generate waste liquid, and input the waste liquid into the waste liquid box.

2. The system according to claim 1, wherein: The reagent reaction device further includes: a first connecting valve; one end of the first connecting valve is connected to the waste liquid box and the reagent kit respectively, and the other end of the first connecting valve is connected to the first end of the flow cell; The flow pool is used to extract the reagent from the reagent kit through the first connecting valve when extracting the reagent from the reagent kit; and to input the waste liquid into the waste liquid box through the first connecting valve when inputting the waste liquid into the waste liquid box.

3. The system according to claim 2, characterized in that The reagent reaction device further includes: a second connecting valve, one end of the second connecting valve is connected to the second end of the flow cell, and the other end of the second connecting valve is connected to the injection pump; The flow cell is used to: input the reagent reaction solution into the syringe pump through the second connecting valve when the reagent reaction solution is input into the syringe pump; When inputting the reagent reaction solution into the flow cell, the injection pump is used to: input the reagent reaction solution into the flow cell through the second connecting valve.

4. The system according to claim 3, characterized in that There are multiple flow cells, multiple second connecting valves and multiple injection pumps; and the number of the second connecting valves and the number of the injection pumps are consistent with the number of the flow cells; each of the second connecting valves is connected to one flow cell and one injection pump.

5. The system according to claim 3, wherein: The reagent reaction device includes multiple groups of circulation modules, each group of circulation modules includes multiple flow cells; the number of the first connecting valves is consistent with the number of groups of the circulation modules, the number of the second connecting valves is consistent with the number of flow cells included in each group of circulation modules; the number of the injection pumps is consistent with the number of the second connecting valves; The plurality of flow cells in each group of flow modules are respectively connected to one of the first connecting valves; Each of the second connecting valves is connected to a flow cell in each group of the flow modules; Each of the second connecting valves is connected to one of the injection pumps.

6. The system according to any one of claims 1 to 5, characterized in that: The system includes: a plurality of reagent kits, wherein the plurality of reagent kits are used to store different reagents; the waste liquid boxes include a plurality of waste liquid boxes, and each reagent kit corresponds to a waste liquid box.

7. The system according to any one of claims 1 to 5, characterized in that: The system further comprises: a pipeline, through which the waste liquid box and the reagent kit are respectively connected to the first end of the flow pool; and the injection pump is connected to the second end of the flow pool through the pipeline.

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