Online enrichment processing system and method of use thereof

Abstract

The present application relates to the technical field of pollutant detection, and provides an online enrichment treatment system and an application method thereof. The treatment system comprises two pump bodies, a first six-way valve, a second six-way valve and an enrichment column. The first six-way valve and the second six-way valve are in fluid communication. One pump body is in fluid communication with the first six-way valve, and the other pump body is in fluid communication with the second six-way valve. The inlet end and the outlet end of the enrichment column are both in fluid communication with the second six-way valve. A quantitative ring is arranged between the two interfaces of the first six-way valve. When the first six-way valve is switched to a first state, the second six-way valve is correspondingly located at a first position, and a sample is injected into the quantitative ring. When the first six-way valve is switched to a second state, the second six-way valve is correspondingly located at a second position. One pump body delivers a mobile phase to flush the quantitative ring, so that the sample is enriched in the enrichment column. When the state of the first six-way valve remains unchanged, the second six-way valve is correspondingly located at the first position, and the other pump body delivers an eluent to elute the enrichment column for online determination, so that small sample amount detection is realized.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of pollutant detection, and in particular to an online enrichment treatment system and an application method thereof. BACKGROUND

[0002] With the development of mining, metallurgy and other industries, a large amount of industrial wastewater containing pollutants is discharged into the water environment, and regional environmental water bodies are occasionally polluted by pollutants. Moreover, pollutants can be significantly enriched in human tissues and organs through the food chain. When the intake of pollutants by the human body exceeds a certain limit, it will cause great harm to human health. Therefore, it is of great significance to carry out daily monitoring of pollutants in environmental water bodies.

[0003] Due to the low concentration of pollutants in environmental water bodies, it is necessary to enrich and concentrate the low-concentration pollutant solution before detection to meet the detection limit requirement. Generally, an enrichment column is made by filling an adsorbent material with pollutant adsorption function into a column. When the low-concentration pollutant solution flows through the enrichment column, the pollutants are adsorbed by the adsorbent material. Then, a small amount of eluent is used to elute the pollutant elements on the adsorbent material, so as to obtain a high-concentration pollutant solution for detection.

[0004] However, the existing online enrichment system has many device components and a complex structure, and cannot form an integrated controllable device. Moreover, it cannot accurately detect small sample quantities.

[0005] In view of this, the present application is proposed. SUMMARY

[0006] The present application provides an online enrichment treatment system to solve the defects of the existing online enrichment system, such as complex device structure and low detection accuracy for small sample quantities, and to realize online enrichment and accurate detection of small sample quantities.

[0007] The present application also provides an application method of the online enrichment treatment system.

[0008] The online enrichment treatment system provided by the present application comprises two pump bodies, a first six-way valve, a second six-way valve and an enrichment column.

[0009] The first six-way valve and the second six-way valve are in fluid communication. One of the pump bodies is in fluid communication with the first six-way valve, and the other pump body is in fluid communication with the second six-way valve. The inlet end and the outlet end of the enrichment column are both in fluid communication with the second six-way valve.

[0010] Wherein, the quantitative ring is arranged between the two interfaces of the first six-way valve, when the first six-way valve is switched to the first state and the second six-way valve is correspondingly located at the first position, the sample is injected into the quantitative ring; when the first six-way valve is switched to the second state and the second six-way valve is correspondingly located at the second position, the pump body in fluid communication with the first six-way valve transports the flow to flush the quantitative ring, so that the sample is enriched to the enrichment column; when the state of the first six-way valve remains unchanged and the second six-way valve is correspondingly located at the first position, the pump body in fluid communication with the second six-way valve transports the eluent to elute the enrichment column for online measurement.

[0011] According to the online enrichment processing system provided by the application, the 1# interface of the first six-way valve is connected with a sample injection device, and the 2# interface is connected with waste liquid;

[0012] When the first six-way valve is switched to the first state, the 6# interface of the first six-way valve is in communication with the 1# interface, the 3# interface is in communication with the 2# interface, the quantitative ring is arranged between the 3# interface and the 6# interface, the 5# interface is in communication with one of the pump bodies, and the 4# interface is in communication with the second six-way valve;

[0013] When the first six-way valve is switched to the second state, the 6# interface of the first six-way valve is in communication with the 5# interface, the 3# interface is in communication with the 4# interface, the 1# interface is in communication with the 2# interface, the quantitative ring is arranged between the 3# interface and the 6# interface, the 5# interface is in communication with one of the pump bodies, and the 4# interface is in communication with the second six-way valve.

[0014] According to the online enrichment processing system provided by the application, the 6# interface of the second six-way valve is in communication with the first six-way valve, the 5# interface is connected with waste liquid, and the 2# interface is connected with a detection device;

[0015] When the second six-way valve is switched to the first state, the 1# interface of the second six-way valve is in communication with the 6# interface, the 4# interface is in communication with the 5# interface, the enrichment column is arranged between the 1# interface and the 4# interface, the 3# interface is in communication with the other pump body, and the 2# interface is in communication with the 3# interface;

[0016] When the second six-way valve is switched to the second state, the 1# interface of the second six-way valve is in communication with the 2# interface, the 3# interface is in communication with the 4# interface, the enrichment column is arranged between the 1# interface and the 4# interface, the 3# interface is in communication with the other pump body, and the 5# interface is in communication with the 6# interface.

[0017] The application further provides an application method of the online enrichment processing system, which comprises the following steps:

[0018] The sample is injected into the quantitative ring through the first six-way valve;

[0019] starting a pump body in fluid communication with the first six-way valve to deliver mobile phase to flush the sample loop and enrich the sample to the first enrichment column;

[0020] starting a pump body in fluid communication with the second six-way valve to deliver eluent to elute the sample from the first enrichment column, suitable for online determination of a sample with a volume less than 15ml.

[0021] The application also provides an online enrichment processing system, comprising two pump bodies, a first six-way valve, a second six-way valve, a third six-way valve, a first enrichment column and a second enrichment column;

[0022] The first six-way valve and the second six-way valve are in fluid communication, one of the pump bodies is in fluid communication with the first six-way valve, the other pump body is in fluid communication with the third six-way valve, the inlet end of the first enrichment column is in fluid communication with the third six-way valve, the outlet end of the first enrichment column is in fluid communication with the second six-way valve, the inlet end of the second enrichment column is in fluid communication with the second six-way valve, and the outlet end of the second enrichment column is in fluid communication with the third six-way valve;

[0023] When the second six-way valve is in the first position and the third six-way valve is in the third position, the pump body in fluid communication with the first six-way valve delivers a second sample to the second enrichment column, and the pump body in fluid communication with the third six-way valve delivers eluent to elute a first sample from the first enrichment column for online determination;

[0024] When the second six-way valve is in the second position and the third six-way valve is in the fourth position, the pump body in fluid communication with the first six-way valve delivers a first sample to the first enrichment column, and the pump body in fluid communication with the third six-way valve delivers eluent to elute a second sample from the second enrichment column for online determination.

[0025] The online enrichment processing system provided by the application is provided with a sample loop between the two interfaces of the first six-way valve;

[0026] When the first six-way valve is switched to the first state, the second six-way valve is correspondingly in the first position, and the third six-way valve is correspondingly in the third position, the sample is injected into the sample loop;

[0027] When the first six-way valve is switched to the second state, the second six-way valve is correspondingly in the second position, and the third six-way valve is correspondingly in the fourth position, the pump body in fluid communication with the first six-way valve delivers mobile phase to flush the sample loop and enrich the sample to the first enrichment column;

[0028] When the first six-way valve is switched to the first state, the second six-way valve is correspondingly located at the first position, and the third six-way valve is correspondingly located at the third position, the pump body in fluid communication with the second six-way valve transports eluent to elute the first enrichment column for online measurement, and meanwhile, the second sample is injected into the quantification ring;

[0029] When the first six-way valve is switched to the second state, the second six-way valve is correspondingly located at the first position, and the third six-way valve is correspondingly located at the third position, the pump body in fluid communication with the first six-way valve transports mobile phase to flush the quantification ring, so that the second sample is enriched to the second enrichment column;

[0030] When the state of the first six-way valve remains unchanged, the second six-way valve is correspondingly located at the second position, and the third six-way valve is correspondingly located at the fourth position, the pump body in fluid communication with the first six-way valve transports mobile phase to flush the quantification ring, so that the first sample is enriched to the first enrichment column, and the pump body in fluid communication with the third six-way valve transports eluent to elute the second enrichment column for online measurement.

[0031] According to the online enrichment processing system provided by the application, the 1# interface of the first six-way valve is connected with a sample injection device, and the 2# interface is connected with waste liquid;

[0032] When the first six-way valve is switched to the first state, the 6# interface of the first six-way valve is in communication with the 1# interface, the 3# interface is in communication with the 2# interface, the quantification ring is arranged between the 3# interface and the 6# interface, the 5# interface is in communication with one of the pump bodies, and the 4# interface is in communication with the second six-way valve;

[0033] When the first six-way valve is switched to the second state, the 6# interface of the first six-way valve is in communication with the 5# interface, the 3# interface is in communication with the 4# interface, the 1# interface is in communication with the 2# interface, the quantification ring is arranged between the 3# interface and the 6# interface, the 5# interface is in communication with one of the pump bodies, and the 4# interface is in communication with the second six-way valve.

[0034] According to the online enrichment processing system provided by the application, the 5# interface of the second six-way valve is in communication with the first six-way valve, and the 2# interface is connected with a detection device;

[0035] When the second six-way valve is located at the first position, the 6# interface of the second six-way valve is in communication with the 5# interface, the 4# interface is in communication with the 6# interface, the 3# interface is in communication with the 4# interface, the 2# interface is in communication with the 1# interface, the inlet end of the second enrichment column is in communication with the 3# interface of the second six-way valve, the outlet end of the second enrichment column is in communication with the third six-way valve, and the 1# interface is in communication with the outlet end of the first enrichment column;

[0036] When the second six-way valve is in the second position, the 1# interface of the second six-way valve is in communication with the 6# interface, the 6# interface is in communication with the 4# interface, the 4# interface is in communication with the 5# interface, the 2# interface is in communication with the 3# interface, the second enrichment column outlet end is in communication with the 3# interface of the second six-way valve, the second enrichment column inlet end is in communication with the third six-way valve, and the 1# interface is in communication with the first enrichment column inlet end.

[0037] According to the online enrichment processing system provided in the application, the 2# interface of the third six-way valve is in communication with one of the pump bodies, and the 5# interface is connected with waste liquid.

[0038] When the third six-way valve is in the third position, the 1# interface of the third six-way valve is in communication with the 2# interface, the 3# interface is in communication with the 4# interface, the 4# interface is in communication with the 6# interface, the 6# interface is in communication with the 5# interface, the second enrichment column outlet end is in communication with the 3# interface, and the first enrichment column inlet end is in communication with the 1# interface.

[0039] When the third six-way valve is in the fourth position, the 2# interface of the third six-way valve is in communication with the 3# interface, the 1# interface is in communication with the 6# interface, the 6# interface is in communication with the 4# interface, the 4# interface is in communication with the 5# interface, the second enrichment column inlet end is in communication with the 3# interface, and the first enrichment column outlet end is in communication with the 1# interface.

[0040] The application further provides an application method of the online enrichment processing system, which comprises the following steps:

[0041] The first sample is injected into the constant volume ring through the first six-way valve;

[0042] The pump body in fluid communication with the first six-way valve is started to transport the mobile phase to flush the constant volume ring, so that the first sample is enriched into the first enrichment column;

[0043] The pump body in fluid communication with the third six-way valve is started to transport the eluent to elute the first enrichment column, and the first sample with a sample amount less than 15ml is subjected to online determination, and at the same time, the second sample is injected into the constant volume ring through the first six-way valve;

[0044] The pump body in fluid communication with the first six-way valve is started to transport the mobile phase to flush the constant volume ring, so that the second sample is enriched into the second enrichment column;

[0045] The pump body in fluid communication with the third six-way valve is started to transport the eluent to elute the second enrichment column, and the second sample with a sample amount less than 15ml is subjected to online determination, and at the same time, the first sample is injected into the constant volume ring through the first six-way valve;

[0046] The above steps are cycled.

[0047] The online enrichment treatment system provided by the application can realize accurate dosing of the sample through the dosing ring when the first six-way valve is switched to the first state and the second six-way valve is located at the first position, so that the online enrichment treatment system can be used for online enrichment and accurate detection of small sample amounts.

[0048] Compared with the prior art, the online enrichment treatment system provided by the application can realize online elution and enrichment detection through switching of the first state and the second state of the first six-way valve and switching of the first position and the second position of the second six-way valve, and the two six-way valves are connected, so that the structure is simple. BRIEF DESCRIPTION OF DRAWINGS

[0049] In order to more clearly illustrate the technical solutions in the application or the prior art, the following will briefly introduce the drawings needed to be used in the embodiments or the prior art description. Obviously, the drawings in the following description are some embodiments of the application, and other drawings can be obtained by those skilled in the art without any creative effort on the basis of these drawings.

[0050] Figure 1 is a structure schematic diagram of the online enrichment treatment system provided by the embodiment 1 in the first state;

[0051] Figure 2 is a structure schematic diagram of the online enrichment treatment system provided by the embodiment 1 in the second state;

[0052] Figure 3 is a structure schematic diagram of the online enrichment treatment system provided by the embodiment 1 in the third state;

[0053] Figure 4 is a structure schematic diagram of the second six-way valve of the online enrichment treatment system provided by the embodiment 2 in the first position;

[0054] Figure 5 is a structure schematic diagram of the second six-way valve of the online enrichment treatment system provided by the embodiment 2 in the second position;

[0055] Figure 6 is a structure schematic diagram of the second six-way valve of the online enrichment treatment system provided by the embodiment 3 in the first position and the third six-way valve in the third position;

[0056] Figure 7 is a structure schematic diagram of the second six-way valve of the online enrichment treatment system provided by the embodiment 3 in the second position and the third six-way valve in the fourth position;

[0057] Figure 8is the first six-way valve of the online enrichment treatment system provided by embodiment 4 of the present application is located in the first position, the second six-way valve is located in the third position and the third six-way valve is located in the fifth position structure schematic diagram;

[0058] Figure 9 is the first six-way valve of the online enrichment treatment system provided by embodiment 4 of the present application is located in the second position, the second six-way valve is located in the fourth position and the third six-way valve is located in the sixth position structure schematic diagram;

[0059] Figure 10 is the first six-way valve of the online enrichment treatment system provided by embodiment 4 of the present application is located in the first position, the second six-way valve is located in the third position and the third six-way valve is located in the fifth position structure schematic diagram;

[0060] Figure 11 is the first six-way valve of the online enrichment treatment system provided by embodiment 4 of the present application is located in the second position, the second six-way valve is located in the third position and the third six-way valve is located in the fifth position structure schematic diagram;

[0061] Figure 12 is the first six-way valve of the online enrichment treatment system provided by embodiment 4 of the present application is located in the second position, the second six-way valve is located in the fourth position and the third six-way valve is located in the sixth position structure schematic diagram;

[0062] Figure 13 is one of the principle structure diagrams of the controller of the online enrichment treatment system provided by the present application;

[0063] Figure 14 is the second principle structure diagram of the controller of the online enrichment treatment system provided by the present application.

[0064] Reference signs:

[0065] 100, first pump body; 200, second pump body; 300, first six-way valve;

[0066] 400, second six-way valve; 500, first enrichment column; 600, quantitative ring;

[0067] 700, third six-way valve; 800, second enrichment column;

[0068] 11, programmable logic controller; 111, power module; 112, central processing unit; 113, storage module; 114, input module; 115, output module; 116, peripheral interface module. DETAILED DESCRIPTION

[0069] In order to make the objects, technical solutions and advantages of the present application clearer, the following will be combined with the accompanying drawings for the purpose of making the technical solutions in the present application clearer, complete and more comprehensible. Obviously, the described embodiments are only a part of the embodiments of the present application, rather than all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those of ordinary skill in the art without creative work fall within the protection scope of the present application.

[0070] In the description of the embodiments of the present application, it should be noted that, unless otherwise explicitly specified and limited, the terms "connected", "connected" should be understood in a broad sense, for example, it can be fixedly connected, or detachably connected, or integrally connected; it can be mechanically connected, or electrically connected; it can be directly connected, or indirectly connected through an intermediate medium. For those of ordinary skill in the art, the specific meanings of the above terms in the embodiments of the present application can be understood according to the specific circumstances.

[0071] In the embodiments of the present application, unless otherwise explicitly specified and limited, the first feature is "on" or "under" the second feature, which can be that the first and second features are in direct contact, or the first and second features are in indirect contact through an intermediate medium. Moreover, the first feature "above", "over" and "on" the second feature can be that the first feature is directly above or obliquely above the second feature, or only means that the horizontal height of the first feature is higher than that of the second feature. The first feature "below", "under" and "under" the second feature can be that the first feature is directly below or obliquely below the second feature, or only means that the horizontal height of the first feature is less than that of the second feature.

[0072] In the description of the present application, the description of the terms "one embodiment", "some embodiments", "example", "specific example" or "some examples" means that the specific features, structures, materials or characteristics described in combination with the embodiment or example are included in at least one embodiment or example of the present application. In the present application, the illustrative description of the above terms does not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described can be combined in any appropriate manner in any one or more embodiments or examples. In addition, those of ordinary skill in the art can combine and combine the different embodiments or examples described in the present application and the features of the different embodiments or examples without contradiction.

[0073] The embodiments of the present application will be described below. Figures 1 to 14 The embodiments of the present application will be described below. It should be understood that the following description is only a schematic embodiment of the present application and does not constitute any limitation on the present application.

[0074] Embodiment 1

[0075] Referring to Figures 1 to 3The online enrichment treatment system comprises a first pump body 100, a second pump body 200, a first six-way valve 300, a second six-way valve 400 and a first enrichment column 500, and the first pump body 100 and the second pump body 200 are both high-pressure pumps;

[0076] The first pump body 100 is in fluid communication with the first six-way valve 300, the second pump body 200 is in fluid communication with the second six-way valve 400, the first six-way valve 300 is in fluid communication with the second six-way valve 400, and the inlet end and the outlet end of the first enrichment column 500 are both in fluid communication with the second six-way valve 400;

[0077] The first six-way valve 300 is adapted to switch between a first state and a second state, and the second six-way valve 400 is adapted to switch between a first position and a second position.

[0078] It should be noted that, in the first state, as shown in Figure 1 , the 1# interface of the first six-way valve 300 is in communication with the 6# interface, the 2# interface is in communication with the 3# interface, the 4# interface is in communication with the 5# interface, and the quantitative ring 600 is connected between the 3# interface and the 6# interface.

[0079] At this time, the second six-way valve 400 is correspondingly in the first position, the 1# interface of the second six-way valve 400 is in communication with the 2# interface, the 3# interface is in communication with the 4# interface, and the 5# interface is in communication with the 6# interface.

[0080] In the second state, as shown in Figure 2 , the 1# interface of the first six-way valve 300 is in communication with the 2# interface, the 3# interface is in communication with the 4# interface, the 5# interface is in communication with the 6# interface, and the quantitative ring 600 is connected between the 3# interface and the 6# interface.

[0081] At this time, the second six-way valve 400 is correspondingly in the second position, the 1# interface of the second six-way valve 400 is in communication with the 6# interface, the 2# interface is in communication with the 3# interface, and the 4# interface is in communication with the 5# interface.

[0082] Referring to Figure 1, specifically, the first six-way valve 300 switches to the first state, the 1# interface of the first six-way valve 300 is connected with the sample inlet device, the 2# interface of the first six-way valve 300 is connected with the waste liquid, the 6# interface of the first six-way valve 300 is communicated with the 1# interface of the first six-way valve 300, the 5# interface of the first six-way valve 300 is communicated with the first pump body 100, the 3# interface of the first six-way valve 300 is communicated with the 2# interface of the first six-way valve 300, the quantitative ring 600 is arranged between the 3# interface of the first six-way valve 300 and the 6# interface of the first six-way valve 300, the sample can enter from the 1# interface of the first six-way valve 300 and enter the quantitative ring 600 through the 6# interface for quantitative sampling, the quantitative ring 600 can accurately sample and is suitable for sampling of a sample less than 15ml.

[0083] Correspondingly, the second six-way valve 400 is located at the first position, the 6# interface of the second six-way valve 400 is communicated with the 5# interface of the second six-way valve 400, the 5# interface of the second six-way valve 400 is connected with the waste liquid, the 6# interface of the second six-way valve 400 is communicated with the 4# interface of the first six-way valve 300. In the first state, the first pump body 100 and the second pump body 200 do not work.

[0084] Referring to Figure 2 , the first six-way valve 300 switches to the second state, the second pump body 200 does not work, the 1# interface of the first six-way valve 300 is connected with the sample inlet device, the 2# interface of the first six-way valve 300 is connected with the waste liquid, the 1# interface of the first six-way valve 300 is communicated with the 2# interface of the first six-way valve 300, the 6# interface of the first six-way valve 300 is communicated with the 5# interface of the first six-way valve 300, the 3# interface of the first six-way valve 300 is communicated with the 4# interface of the first six-way valve 300, the quantitative ring 600 is arranged between the 3# interface of the first six-way valve 300 and the 6# interface of the first six-way valve 300, the 5# interface of the first six-way valve 300 is communicated with the first pump body 100, the 4# interface of the first six-way valve 300 is communicated with the 6# interface of the second six-way valve 400, so that the first pump body 100 transports the mobile phase to flush the quantitative ring 600 and enriches the sample to the first enrichment column 500.

[0085] Correspondingly, the second six-way valve 400 is located at the second position, the 1# interface of the second six-way valve 400 is communicated with the 6# interface of the second six-way valve 400, the 4# interface of the second six-way valve 400 is communicated with the 5# interface of the second six-way valve 400, the first enrichment column 500 is arranged between the 1# interface of the second six-way valve 400 and the 4# interface of the second six-way valve 400, the 3# interface of the second six-way valve 400 is communicated with the second pump body 200, the 2# interface of the second six-way valve 400 is communicated with the 3# interface of the second six-way valve 400, and the 2# interface of the second six-way valve 400 is connected with the detection device.

[0086] In the second state, the first pump body 100 delivers the mobile phase to flush the quantitative ring 600, and the fluid flows into the inflow end of the first enrichment column 500 through the 6# interface of the second six-way valve 400 and the 1# interface of the second six-way valve 400, is enriched by the first enrichment column 500, and then flows into the 4# interface of the second six-way valve 400 from the outflow end of the first enrichment column 500, and finally is discharged through the 5# interface of the second six-way valve 400, thereby completing the enrichment of the sample.

[0087] Referring to Figure 3 , the first six-way valve 300 is kept in the second state, and correspondingly, the second six-way valve 400 is located at the first position. The first pump body 100 is not working, the 1# interface of the second six-way valve 400 is communicated with the 2# interface of the second six-way valve 400, the 3# interface of the second six-way valve 400 is communicated with the 4# interface of the second six-way valve 400, the first enrichment column 500 is arranged between the 1# interface of the second six-way valve 400 and the 4# interface of the second six-way valve 400, the 3# interface of the second six-way valve 400 is communicated with the second pump body 200, the 2# interface of the second six-way valve 400 is connected with the detection device, and the 5# interface of the second six-way valve 400 is communicated with the 6# interface of the second six-way valve 400.

[0088] In the second state, the second pump body 200 delivers the eluent to flush the first enrichment column 500, and the fluid flows into the inflow end of the first enrichment column 500 through the 3# interface of the second six-way valve 400 and the 4# interface of the second six-way valve 400, and then flows into the 1# interface of the second six-way valve 400 from the outflow end of the first enrichment column 500 after the sample enriched by the first enrichment column 500 is eluted, and finally flows into the detection device through the 2# interface of the second six-way valve 400, thereby completing the elution and online detection of the sample.

[0089] Based on the above online enrichment processing system, the application further provides an application method of the online enrichment processing system, which comprises the following steps:

[0090] The sample is introduced into the quantitative ring 600 through the first six-way valve 300;

[0091] The pump body in fluid communication with the first six-way valve 300 is started to deliver the mobile phase to flush the quantitative ring 600, so that the sample is enriched to the enrichment column 500;

[0092] The pump body in fluid communication with the second six-way valve 400 is started to deliver the eluent to elute the enrichment column 500 and perform online detection, and the sample with an amount less than 15 ml is suitable for online determination.

[0093] Compared with the prior art online enrichment processing system, the online enrichment processing system structure of the present application switches the first six-way valve 300 between the first state and the second state, and the second six-way valve 400 between the first position and the second position, thereby constituting three states of the online enrichment processing system, so as to realize accurate quantitative sampling of small samples, online enrichment and elution detection, and the structure is simple.

[0094] Embodiment 2

[0095] Referring to Figures 4 to 5 In some embodiments of the present application, the online enrichment processing system comprises a first pump body 100, a second pump body 200, a first six-way valve 300, a second six-way valve 400, a third six-way valve 700, and a first enrichment column 500.

[0096] Referring to Figure 4 When the first six-way valve 300 and the third six-way valve 700 are not working, and the second six-way valve 400 is located at the first position, the 5# interface of the second six-way valve 400 is connected with waste liquid, the 6# interface of the second six-way valve 400 is connected with the first pump body 100, the 1# interface of the second six-way valve 400 is communicated with the 6# interface of the second six-way valve 400, the 2# interface of the second six-way valve 400 is communicated with the 3# interface of the second six-way valve 400, the 3# interface of the second six-way valve 400 is connected with the second pump body 200, the 4# interface of the second six-way valve 400 is communicated with the 5# interface of the second six-way valve 400, one end of the first enrichment column 500 is connected with the 1# interface of the second six-way valve 400, and the other end of the first enrichment column 500 is connected with the 4# interface of the second six-way valve 400, the first pump body 100 is started, the sample is enriched in the first enrichment column 500 through the 6# interface and the 1# interface of the second six-way valve 400, and the waste liquid flows out through the 4# interface and the 5# interface of the second six-way valve 400.

[0097] Referring to Figure 5When the first six-way valve 300 and the third six-way valve 700 are not working, and the second six-way valve 400 is located at the second position, the 1# interface of the second six-way valve 400 is in communication with the 2# interface of the second six-way valve 400, the 3# interface of the second six-way valve 400 is in communication with the 4# interface of the second six-way valve 400, the 5# interface of the second six-way valve 400 is in communication with the 6# interface of the second six-way valve 400, the first pump body 100 is connected with the 6# interface of the second six-way valve 400, the 5# interface of the second six-way valve 400 is connected with waste liquid, the 2# interface of the second six-way valve 400 is connected with a detection device, the second pump body 200 is connected with the 3# interface of the second six-way valve 400, one end of the first enrichment column 500 is connected with the 4# interface of the second six-way valve 400, and the other end of the first enrichment column 500 is connected with the 1# interface of the second six-way valve 400, the second pump body 200 is started, the pump moves the eluent to the first enrichment column 500 through the 3# interface and the 4# interface of the second six-way valve 400, the first enrichment column 500 is eluted, the eluent flows into the detection device through the 2# interface and the 1# interface of the second six-way valve 400, and the online detection is completed. In this embodiment, one six-way valve can complete online detection, is suitable for single sample enrichment, and is suitable for large-volume samples.

[0098] Embodiment 3

[0099] Referring to Figures 6 to 7 The online enrichment processing system comprises a first pump body 100, a second pump body 200, a first six-way valve 300, a second six-way valve 400, a third six-way valve 700, a first enrichment column 500, and a second enrichment column 800;

[0100] The first pump body 100 is in fluid communication with the first six-way valve 300, the second pump body 200 is in fluid communication with the third six-way valve 700, the first six-way valve 300 is in fluid communication with the second six-way valve 400, two ends of the first enrichment column 500 are in fluid communication with the second six-way valve 400 and the third six-way valve 700 respectively, and two ends of the second enrichment column 800 are in fluid communication with the second six-way valve 400 and the third six-way valve 700 respectively;

[0101] Wherein, the first six-way valve 300 is unchanged in position, the second six-way valve 400 is adapted to switch between the first position and the second position, and the third six-way valve 700 is adapted to switch between the third position and the fourth position;

[0102] Referring to Figure 6 , the first six-way valve 300 is unchanged in position, the second six-way valve 400 is located at the first position, the third six-way valve 700 is located at the third position, the first pump body 100 transports the mobile phase to flush the second enrichment column 800 through the first six-way valve 300 and the second six-way valve 400, so that the sample is enriched to the second enrichment column 800, and at the same time, the second pump body 200 transports the eluent to elute the first enrichment column 500 through the third six-way valve 700, so as to complete online detection.

[0103] Referring to Figure 7 , the first six-way valve 300 is in a fixed position, the second six-way valve 400 is in a second position, and the third six-way valve 700 is in a fourth position. The first pump body 100 delivers the mobile phase through the first six-way valve 300 and the second six-way valve 400 to flush the first enrichment column 500, and the sample is enriched in the first enrichment column 500. At the same time, the second pump body 200 delivers the eluent through the third six-way valve 700 to elute the second enrichment column 800, so as to complete the online detection.

[0104] In this embodiment, the first six-way valve 300 is in a fixed position, and the second six-way valve 400 and the third six-way valve 700 are in variable positions. The online enrichment detection of two samples can be realized, and the use of a quantitative ring for sampling is not required. This embodiment is suitable for large-volume samples.

[0105] It can be understood that, referring to Figure 6 , when the first six-way valve 300 is in a fixed position, the second six-way valve 400 is in a first position, and the third six-way valve 700 is in a third position, the 1# interface of the first six-way valve 300 is in communication with the 6# interface of the first six-way valve 300, the 2# interface of the first six-way valve 300 is in communication with the 3# interface of the first six-way valve 300, the two ends of the quantitative ring 600 are connected to the 3# interface and the 6# interface of the first six-way valve 300, respectively, the 4# interface of the first six-way valve 300 is in communication with the 5# interface of the first six-way valve 300, the 5# interface of the first six-way valve 300 is in communication with the first pump body 100, the 4# interface of the first six-way valve 300 is in communication with the 5# interface of the second six-way valve 400, the 6# interface of the second six-way valve 400 is in communication with the 5# interface of the second six-way valve 400, the 4# interface of the second six-way valve 400 is in communication with the 6# interface of the second six-way valve 400, the 3# interface of the second six-way valve 400 is in communication with the 4# interface of the second six-way valve 400, the 3# interface of the second six-way valve 400 is connected to the inlet end of the second enrichment column 800, the outlet end of the second enrichment column 800 is connected to the 3# interface of the third six-way valve 700, the 4# interface of the third six-way valve 700 is in communication with the 3# interface of the third six-way valve 700, the 6# interface of the third six-way valve 700 is in communication with the 4# interface of the third six-way valve 700, the 5# interface of the third six-way valve 700 is in communication with the 6# interface of the third six-way valve 700, the 5# interface of the third six-way valve 700 is connected to a waste liquid, the 2# interface of the third six-way valve 700 is connected to the second pump body 200, the 1# interface of the third six-way valve 700 is in communication with the 2# interface of the third six-way valve 700, the inlet end of the first enrichment column 500 is connected to the 1# interface of the third six-way valve 700, the outlet end of the first enrichment column 500 is connected to the 1# interface of the second six-way valve 400, the 2# interface of the second six-way valve 400 is in communication with the 1# interface of the second six-way valve 400, and the 2# interface of the second six-way valve 400 is connected to a detection device.

[0106] The first pump body 100 transports the mobile phase through the 5# interface of the first six-way valve 300 and the 4# interface of the first six-way valve 300 into the 5# interface of the second six-way valve 400, and then through the 6# interface of the second six-way valve 400, the 4# interface of the second six-way valve 400 and the 3# interface of the second six-way valve 400, and finally into the second enrichment column 800 to enrich the sample to the second enrichment column 800, and the waste liquid flows through the 3# interface of the third six-way valve 700, the 4# interface of the third six-way valve 700, the 6# interface of the third six-way valve 700 and the 5# interface of the third six-way valve 700, and finally into the waste liquid treatment.

[0107] The second pump body 200 transports the eluent through the 2# interface of the third six-way valve 700 and the 1# interface of the third six-way valve 700 into the first enrichment column 500, elutes the first enrichment column 500, and the eluent flows to the 1# interface of the second six-way valve 400 and the 2# interface of the second six-way valve 400 to complete the online detection.

[0108] Referring to Figure 7, the first six-way valve 300 is unchanged, the second six-way valve 400 is located in the second position, and the third six-way valve 700 is located in the fourth position, the 1# interface of the first six-way valve 300 is in communication with the 6# interface of the first six-way valve 300, the 2# interface of the first six-way valve 300 is in communication with the 3# interface of the first six-way valve 300, the two ends of the constant flow ring 600 are connected with the 3# interface and the 6# interface of the first six-way valve 300 respectively, the 4# interface of the first six-way valve 300 is in communication with the 5# interface of the first six-way valve 300, the 5# interface of the first six-way valve 300 is in communication with the first pump body 100, the 4# interface of the first six-way valve 300 is in communication with the 5# interface of the second six-way valve 400, the 4# interface of the second six-way valve 400 is in communication with the 5# interface of the second six-way valve 400, the 6# interface of the second six-way valve 400 is in communication with the 4# interface of the second six-way valve 400, the 1# of the second six-way valve 400 is in communication with the 6# of the second six-way valve 400, the 2# interface of the second six-way valve 400 is in communication with the 3# interface of the second six-way valve 400, the 1# interface of the second six-way valve 400 is connected with the inlet end of the first enrichment column 500, the outlet end of the first enrichment column 500 is connected with the 1# interface of the third six-way valve 700, the 6# interface of the third six-way valve 700 is in communication with the 1# interface of the third six-way valve 700, the 4# interface of the third six-way valve 700 is in communication with the 6# interface of the third six-way valve 700, the 5# interface of the third six-way valve 700 is in communication with the 4# interface of the third six-way valve 700, the 5# interface of the third six-way valve 700 is connected with waste liquid, the 2# interface of the third six-way valve 700 is connected with the second pump body 200, the 3# interface of the third six-way valve 700 is in communication with the 2# interface of the third six-way valve 700, the inlet end of the second enrichment column 800 is connected with the 3# interface of the third six-way valve 700, the outlet end of the second enrichment column 800 is connected with the 3# interface of the second six-way valve 400, and the 2# interface of the second six-way valve 400 is connected with a detection device.

[0109] The first pump body 100 transports the mobile phase through the 5# interface of the first six-way valve 300 and the 4# interface of the first six-way valve 300 into the 5# interface of the second six-way valve 400, and then through the 4# interface of the second six-way valve 400, the 6# interface of the second six-way valve 400 and the 1# interface of the second six-way valve 400, and then into the first enrichment column 500 to enrich the sample to the first enrichment column 500, and the waste liquid flows through the outlet end of the first enrichment column 500 to the 1# interface of the third six-way valve 700, the 6# interface of the third six-way valve 700, the 4# interface of the third six-way valve 700 and the 5# interface of the third six-way valve 700, and finally flows into the waste liquid treatment.

[0110] The second pump body 200 transports the eluent through the 2# interface of the third six-way valve 700 and the 3# interface of the third six-way valve 700 into the second enrichment column 800, elutes the second enrichment column 800, and the eluent flows to the 3# interface of the second six-way valve 400 and the 2# interface of the second six-way valve 400 to complete the online detection.

[0111] Embodiment 4

[0112] Referring to Figures 8 to 12 The online enrichment processing system comprises a first pump body 100, a second pump body 200, a first six-way valve 300, a second six-way valve 400, a third six-way valve 700, a first enrichment column 500 and a second enrichment column 800;

[0113] The first pump body 100 is in fluid communication with the first six-way valve 300, the second pump body 200 is in fluid communication with the third six-way valve 700, the first six-way valve 300 is in fluid communication with the second six-way valve 400, an inlet end of the first enrichment column 500 is in fluid communication with the third six-way valve 700, an outlet end of the first enrichment column 500 is in fluid communication with the second six-way valve 400, an inlet end of the second enrichment column 800 is in fluid communication with the second six-way valve 400, and an outlet end of the second enrichment column 800 is in fluid communication with the third six-way valve 700;

[0114] Wherein, a dosing ring 600 is arranged between two interfaces of the first six-way valve 300, the first six-way valve 300 is adapted to switch between a first position and a second position, the second six-way valve 400 is adapted to switch between a third position and a fourth position, and the third six-way valve 700 is adapted to switch between a fifth position and a sixth position;

[0115] Referring to Figure 8 , the first six-way valve 300 is located at the first position, the second six-way valve 400 is located at the third position, the third six-way valve 700 is located at the fifth position, the 1# interface of the first six-way valve 300 is in communication with the 6# interface of the first six-way valve 300, the 2# interface of the first six-way valve 300 is in communication with the 3# interface of the first six-way valve 300, the 4# interface of the first six-way valve 300 is in communication with the 5# interface of the first six-way valve 300, and the two ends of the dosing ring 600 are connected with the 3# interface and the 6# interface of the first six-way valve 300 respectively; the 1# interface of the second six-way valve 400 is in communication with the 2# interface of the second six-way valve 400, the 3# interface of the second six-way valve 400 is in communication with the 4# interface of the second six-way valve 400, the 4# interface of the second six-way valve 400 is in communication with the 6# interface of the second six-way valve 400, the 6# interface of the second six-way valve 400 is in communication with the 5# interface of the second six-way valve 400; the 1# interface of the third six-way valve 700 is in communication with the 2# interface of the third six-way valve 700, the 3# interface of the third six-way valve 700 is in communication with the 4# interface of the third six-way valve 700, the 4# interface of the third six-way valve 700 is in communication with the 6# interface of the third six-way valve 700, the 6# interface of the third six-way valve 700 is in communication with the 5# interface of the third six-way valve 700, and the first sample is injected into the dosing ring 600;

[0116] Referring to Figure 9, the first six-way valve 300 is located in the second position, the second six-way valve 400 is located in the fourth position, the third six-way valve 700 is located in the sixth position, the 1# interface of the first six-way valve 300 is communicated with the 2# interface of the first six-way valve 300, the 3# interface of the first six-way valve 300 is communicated with the 4# interface of the first six-way valve 300, the 6# interface of the first six-way valve 300 is communicated with the 5# interface of the first six-way valve 300, and the two ends of the quantitative ring 600 are connected with the 3# interface and the 6# interface of the first six-way valve 300 respectively; the 1# interface of the second six-way valve 400 is communicated with the 6# interface of the second six-way valve 400, the 4# interface of the second six-way valve 400 is communicated with the 6# interface of the second six-way valve 400, the 5# interface of the second six-way valve 400 is communicated with the 4# interface of the second six-way valve 400, and the 3# interface of the second six-way valve 400 is communicated with the 2# interface of the second six-way valve 400; the 1# interface of the third six-way valve 700 is communicated with the 6# interface of the third six-way valve 700, the 6# interface of the third six-way valve 700 is communicated with the 4# interface of the third six-way valve 700, the 4# interface of the third six-way valve 700 is communicated with the 5# interface of the third six-way valve 700, the 3# interface of the third six-way valve 700 is communicated with the 2# interface of the third six-way valve 700, and the first pump body 100 transports the mobile phase to flush the quantitative ring 600, so that the first sample is enriched to the first enrichment column 500;

[0117] Referring to Figure 10 , the first six-way valve 300 is located in the first position, the second six-way valve 400 is located in the third position, the third six-way valve 700 is located in the fifth position, the 1# interface of the first six-way valve 300 is communicated with the 6# interface of the first six-way valve 300, the 2# interface of the first six-way valve 300 is communicated with the 3# interface of the first six-way valve 300, the 4# interface of the first six-way valve 300 is communicated with the 5# interface of the first six-way valve 300, and the two ends of the quantitative ring 600 are connected with the 3# interface and the 6# interface of the first six-way valve 300 respectively; the 1# interface of the second six-way valve 400 is communicated with the 2# interface of the second six-way valve 400, the 3# interface of the second six-way valve 400 is communicated with the 4# interface of the second six-way valve 400, the 4# interface of the second six-way valve 400 is communicated with the 6# interface of the second six-way valve 400, and the 6# interface of the second six-way valve 400 is communicated with the 5# interface of the second six-way valve 400; the 1# interface of the third six-way valve 700 is communicated with the 2# interface of the third six-way valve 700, the 3# interface of the third six-way valve 700 is communicated with the 4# interface of the third six-way valve 700, the 4# interface of the third six-way valve 700 is communicated with the 6# interface of the third six-way valve 700, and the 6# interface of the third six-way valve 700 is communicated with the 5# interface of the third six-way valve 700, and the second pump body 200 transports the eluent to elute the first enrichment column 500 to realize online determination, and at the same time, the second sample is injected into the quantitative ring 600;

[0118] Referring to Figure 11, the first six-way valve 300 is located in the second position, the second six-way valve 400 is located in the third position, the third six-way valve 700 is located in the fifth position, the 1# interface of the first six-way valve 300 communicates with the 2# interface of the first six-way valve 300, the 3# interface of the first six-way valve 300 communicates with the 4# interface of the first six-way valve 300, the 5# interface of the first six-way valve 300 communicates with the 6# interface of the first six-way valve 300, and the two ends of the constant volume ring 600 are connected with the 3# interface and the 6# interface of the first six-way valve 300 respectively; the 1# interface of the second six-way valve 400 communicates with the 2# interface of the second six-way valve 400, the 3# interface of the second six-way valve 400 communicates with the 4# interface of the second six-way valve 400, the 4# interface of the second six-way valve 400 communicates with the 6# interface of the second six-way valve 400, and the 6# interface of the second six-way valve 400 communicates with the 5# interface of the second six-way valve 400; the 1# interface of the third six-way valve 700 communicates with the 2# interface of the third six-way valve 700, the 3# interface of the third six-way valve 700 communicates with the 4# interface of the third six-way valve 700, the 4# interface of the third six-way valve 700 communicates with the 6# interface of the third six-way valve 700, and the 6# interface of the third six-way valve 700 communicates with the 5# interface of the third six-way valve 700, and the first pump body 100 delivers the mobile phase to flush the constant volume ring 600, so that the second sample is enriched to the second enrichment column 800.

[0119] Referring to Figure 12 , the first six-way valve 300 is located in the second position, the second six-way valve 400 is located in the fourth position, the third six-way valve 700 is located in the sixth position, the 1# interface of the first six-way valve 300 communicates with the 2# interface of the first six-way valve 300, the 3# interface of the first six-way valve 300 communicates with the 4# interface of the first six-way valve 300, the 6# interface of the first six-way valve 300 communicates with the 5# interface of the first six-way valve 300, and the two ends of the constant volume ring 600 are connected with the 3# interface and the 6# interface of the first six-way valve 300 respectively; the 1# interface of the second six-way valve 400 communicates with the 6# interface of the second six-way valve 400, the 4# interface of the second six-way valve 400 communicates with the 6# interface of the second six-way valve 400, the 5# interface of the second six-way valve 400 communicates with the 4# interface of the second six-way valve 400, and the 3# interface of the second six-way valve 400 communicates with the 2# interface of the second six-way valve 400; the 1# interface of the third six-way valve 700 communicates with the 6# interface of the third six-way valve 700, the 6# interface of the third six-way valve 700 communicates with the 4# interface of the third six-way valve 700, the 4# interface of the third six-way valve 700 communicates with the 5# interface of the third six-way valve 700, the 3# interface of the third six-way valve 700 communicates with the 2# interface of the third six-way valve 700, and the second pump body 200 delivers the eluent to elute the second enrichment column 800 to realize online determination, and at the same time, the first pump body 100 delivers the mobile phase to flush the constant volume ring 600 so that the first sample is enriched to the first enrichment column 500.

[0120] It can be understood that, referring to Figure 8 or Figure 10 , when the first six-way valve 300 is in the first position, the 6# interface of the first six-way valve 300 is in communication with the 1# interface of the first six-way valve 300, the 3# interface of the first six-way valve 300 is in communication with the 2# interface of the first six-way valve 300, the dosing ring 600 is arranged between the 3# interface of the first six-way valve 300 and the 6# interface of the first six-way valve 300, the 5# interface of the first six-way valve 300 is in communication with the first pump body 100, the 4# interface of the first six-way valve 300 is in communication with the second six-way valve 400, and the sample is injected into the dosing ring 600. In this embodiment, the sample can be suitable for a sample with a sample amount less than 15 ml;

[0121] Referring to Figure 10 , when the first six-way valve 300 is in the first position, the second six-way valve 400 is in the third position, and the third six-way valve 700 is in the fifth position; the 5# interface of the second six-way valve 400 is in communication with the 4# interface of the first six-way valve 300, the 6# interface of the second six-way valve 400 is in communication with the 5# interface of the second six-way valve 400, the 4# interface of the second six-way valve 400 is in communication with the 6# interface of the second six-way valve 400, the 3# interface of the second six-way valve 400 is in communication with the 4# interface of the second six-way valve 400, the 1# interface of the second six-way valve 400 is in communication with the 2# interface of the second six-way valve 400, the 1# interface of the second six-way valve 400 is connected with the outlet end of the first enrichment column 500, the 2# interface of the second six-way valve 400 is connected with the detection device, and the 3# interface of the second six-way valve 400 is connected with the inlet end of the second enrichment column 800; the 1# interface of the third six-way valve 700 is connected with the inlet end of the first enrichment column 500, the 2# interface of the third six-way valve 700 is in communication with the 1# interface of the third six-way valve 700, the 2# interface of the third six-way valve 700 is connected with the second pump body 200, the 3# interface of the third six-way valve 700 is connected with the outlet end of the second enrichment column 800, the 4# interface of the third six-way valve 700 is in communication with the 3# interface of the third six-way valve 700, the 6# interface of the third six-way valve 700 is in communication with the 4# interface of the third six-way valve 700, the 5# interface of the third six-way valve 700 is in communication with the 6# interface of the third six-way valve 700, and the 5# interface of the third six-way valve 700 is connected with the waste liquid. The second pump body 200 transports the eluent to elute the first enrichment column 500 to realize online measurement, and at the same time, the second sample is injected into the dosing ring 600.

[0122] Referring to Figure 9 or Figure 11 or Figure 12, the 6# interface of the first six-way valve 300 communicates with the 5# interface of the first six-way valve 300, the 3# interface of the first six-way valve 300 communicates with the 4# interface of the first six-way valve 300, the 1# interface of the first six-way valve 300 communicates with the 2# interface of the first six-way valve 300, the constant flow ring 600 is arranged between the 3# interface of the first six-way valve 300 and the 6# interface of the first six-way valve 300, the 5# interface of the first six-way valve 300 is connected with the first pump body 100, and the 4# interface of the first six-way valve 300 communicates with the second six-way valve 400, so as to convey the mobile phase to flush the constant flow ring 600.

[0123] With reference to Figure 9 , the first six-way valve 300 is located at the second position, the second six-way valve 400 is located at the fourth position, and the third six-way valve 700 is located at the sixth position; the 5# interface of the second six-way valve 400 communicates with the 4# interface of the first six-way valve 300, the 4# interface of the second six-way valve 400 communicates with the 5# interface of the second six-way valve 400, the 6# interface of the second six-way valve 400 communicates with the 4# interface of the second six-way valve 400, the 1# interface of the second six-way valve 400 communicates with the 6# interface of the second six-way valve 400, the 2# interface of the second six-way valve 400 communicates with the 3# interface of the second six-way valve 400, the 1# interface of the second six-way valve 400 is connected with the inlet end of the first enrichment column 500, the 2# interface of the second six-way valve 400 is connected with the detection equipment, the 3# interface of the second six-way valve 400 is connected with the outlet end of the second enrichment column 800; the 1# interface of the third six-way valve 700 is connected with the outlet end of the first enrichment column 500, the 6# interface of the third six-way valve 700 communicates with the 1# interface of the third six-way valve 700, the 4# interface of the third six-way valve 700 communicates with the 6# interface of the third six-way valve 700, the 5# interface of the third six-way valve 700 communicates with the 4# interface of the third six-way valve 700, the 2# interface of the third six-way valve 700 is connected with the second pump body 200, the 3# interface of the third six-way valve 700 is connected with the inlet end of the second enrichment column 800, and the 5# interface of the third six-way valve 700 is connected with the waste liquid. The first pump body 100 conveys the mobile phase to flush the constant flow ring 600, so that the first sample is enriched to the first enrichment column 500.

[0124] With reference to Figure 11, the first six-way valve 300 is located in the second position, the second six-way valve 400 is located in the third position, and the third six-way valve 700 is located in the fifth position; the 5# interface of the second six-way valve 400 is in communication with the 4# interface of the first six-way valve 300, the 6# interface of the second six-way valve 400 is in communication with the 5# interface of the second six-way valve 400, the 4# interface of the second six-way valve 400 is in communication with the 6# interface of the second six-way valve 400, the 3# interface of the second six-way valve 400 is in communication with the 4# interface of the second six-way valve 400, the 1# interface of the second six-way valve 400 is in communication with the 2# interface of the second six-way valve 400, the 1# interface of the second six-way valve 400 is connected with the outlet end of the first enrichment column 500, the 2# interface of the second six-way valve 400 is connected with the detection device, and the 3# interface of the second six-way valve 400 is connected with the inlet end of the second enrichment column 800; the 1# interface of the third six-way valve 700 is connected with the inlet end of the first enrichment column 500, the 2# interface of the third six-way valve 700 is in communication with the 1# interface of the third six-way valve 700, the 2# interface of the third six-way valve 700 is connected with the second pump body 200, the 3# interface of the third six-way valve 700 is connected with the outlet end of the second enrichment column 800, the 4# interface of the third six-way valve 700 is in communication with the 3# interface of the third six-way valve 700, the 6# interface of the third six-way valve 700 is in communication with the 4# interface of the third six-way valve 700, the 5# interface of the third six-way valve 700 is in communication with the 6# interface of the third six-way valve 700, and the 5# interface of the third six-way valve 700 is connected with the waste liquid. The second pump body 200 transports the eluent to elute the first enrichment column 500 to realize online determination, and at the same time, the second sample is injected into the quantitative ring 600. The first pump body 100 transports the mobile phase to flush the quantitative ring 600, so that the second sample is enriched to the second enrichment column 800.

[0125] Referring to Figure 12, the first six-way valve 300 is located in the second position, the second six-way valve 400 is located in the fourth position, and the third six-way valve 700 is located in the sixth position; the 5# interface of the second six-way valve 400 is in communication with the 4# interface of the first six-way valve 300, the 4# interface of the second six-way valve 400 is in communication with the 5# interface of the second six-way valve 400, the 6# interface of the second six-way valve 400 is in communication with the 4# interface of the second six-way valve 400, the 1# interface of the second six-way valve 400 is in communication with the 6# interface of the second six-way valve 400, the 2# interface of the second six-way valve 400 is in communication with the 3# interface of the second six-way valve 400, the 1# interface of the second six-way valve 400 is connected with the inlet end of the first enrichment column 500, the 2# interface of the second six-way valve 400 is connected with the detection device, the 3# interface of the second six-way valve 400 is connected with the outlet end of the second enrichment column 800; the 1# interface of the third six-way valve 700 is connected with the outlet end of the first enrichment column 500, the 6# interface of the third six-way valve 700 is in communication with the 1# interface of the third six-way valve 700, the 4# interface of the third six-way valve 700 is in communication with the 6# interface of the third six-way valve 700, the 5# interface of the third six-way valve 700 is in communication with the 4# interface of the third six-way valve 700, the 2# interface of the third six-way valve 700 is connected with the second pump body 200, the 3# interface of the third six-way valve 700 is connected with the inlet end of the second enrichment column 800, and the 5# interface of the third six-way valve 700 is connected with the waste liquid. The second pump body 200 transports the eluent to elute the second enrichment column 800 to realize online measurement, and at the same time, the first pump body 100 transports the mobile phase to flush the quantitative ring 600 so that the first sample is enriched to the first enrichment column 500.

[0126] The embodiment of the present application also provides an application method of the online enrichment processing system, based on any one of the online enrichment processing systems, comprising the following steps:

[0127] The first sample is injected into the quantitative ring 600;

[0128] The first pump body 100 is started to transport the mobile phase to flush the quantitative ring 600, so that the first sample is enriched to the first enrichment column 500;

[0129] The second pump body 200 is started to transport the eluent to elute the first enrichment column 500, so that online measurement is realized, and at the same time, the second sample is injected into the quantitative ring 600;

[0130] The first pump body 100 is started to transport the mobile phase to flush the quantitative ring 600, so that the second sample is enriched to the second enrichment column 800;

[0131] The second pump body 200 is started to transport the eluent to elute the second enrichment column 800, so that online measurement is realized, and at the same time, the first sample is injected into the quantitative ring 600;

[0132] The above steps are cycled.

[0133] The embodiment can realize accurate sampling through a quantitative loop, and is also applicable to samples with a sample amount less than 15 ml. The switching between the states of the three six-way valves can enable online enrichment of two samples.

[0134] Referring to Figure 13 In some embodiments of the present application, the online enrichment processing system further comprises a programmable logic controller 11, which automatically controls the states and position switching of the components in the online enrichment processing system.

[0135] The first pump body 100, the second pump body 200, the first six-way valve 300, the second six-way valve 400 and the third six-way valve 700 are respectively electrically connected with the programmable logic controller 11, which can control the start and stop of the first pump body 100 and the second pump body 200, and can control the switching of the first six-way valve 300 between the first position and the second position, the switching of the second six-way valve 400 between the third position and the fourth position, and the switching of the third six-way valve 700 between the fifth position and the sixth position. Under a preset program, a plurality of different control programs can be combined to meet the detection needs of the online enrichment processing system.

[0136] Referring to Figure 14 Specifically, in some embodiments of the present application, the programmable logic controller 11 comprises a power module 111, a central processing unit 112, a storage module 113, an input module 114, an output module 115 and a peripheral interface module 116. The power module 111, the storage module 113, the input module 114, the output module 115 and the peripheral interface module 116 are respectively electrically connected with the central processing unit 112, and the first pump body 100, the second pump body 200, the first six-way valve 300, the second six-way valve 400 and the third six-way valve 700 are respectively electrically connected with the output module 115.

[0137] The peripheral interface module 116 can be connected with a computer or the like to write an online enrichment process program into the programmable logic controller 11. The online enrichment process program written into the programmable logic controller 11 is stored in the storage module 113. When started, the central processing unit 112 calls the online enrichment process program to output a signal from the output module 115 to drive the first pump body 100, the second pump body 200, the first six-way valve 300, the second six-way valve 400 and the third six-way valve 700 to work.

[0138] In some embodiments of the present application, the output module 115 can be a pulse output unit. That is, the programmable logic controller 11 can utilize a pulse signal to drive the first pump body 100, the second pump body 200, the first six-way valve 300, the second six-way valve 400 and the third six-way valve 700 to work.

[0139] In some embodiments of the present application, the output module 115 can also be a digital output unit. That is, the programmable logic controller 11 can use digital signals to drive the first pump body 100, the second pump body 200, the first six-way valve 300, the second six-way valve 400 and the third six-way valve 700 to work.

[0140] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present application, and not to limit them; although the present application has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that they can still modify the technical solutions recorded in the foregoing embodiments, or make equivalent replacements for some technical features; and these modifications or replacements do not make the essence of the corresponding technical solutions deviate from the spirit and scope of the technical solutions of the embodiments of the present application.

Claims

1. An application method of an online enrichment processing system, characterized by, The online enrichment processing system comprises two pump bodies, a first six-way valve, a second six-way valve, a third six-way valve, a first enrichment column and a second enrichment column; The first six-way valve and the second six-way valve are in fluid communication, one of the pump bodies is in fluid communication with the first six-way valve, the other pump body is in fluid communication with the third six-way valve, the inlet end of the first enrichment column is in fluid communication with the third six-way valve, the outlet end of the first enrichment column is in fluid communication with the second six-way valve, the inlet end of the second enrichment column is in fluid communication with the second six-way valve, and the outlet end of the second enrichment column is in fluid communication with the third six-way valve; When the second six-way valve is in the first position and the third six-way valve is in the third position, the pump body in fluid communication with the first six-way valve transports the second sample to the second enrichment column for enrichment, and the pump body in fluid communication with the third six-way valve transports the eluent to elute the first sample of the first enrichment column for online determination; When the second six-way valve is in the second position and the third six-way valve is in the fourth position, the pump body in fluid communication with the first six-way valve transports the first sample to the first enrichment column for enrichment, and the pump body in fluid communication with the third six-way valve transports the eluent to elute the second sample of the second enrichment column for online determination; The application method comprises the following steps: The first sample is injected into the quantification ring through the first six-way valve; The pump body in fluid communication with the first six-way valve is started to transport the mobile phase to flush the quantification ring, so that the first sample is enriched in the first enrichment column; The pump body in fluid communication with the third six-way valve is started to transport the eluent to elute the first enrichment column, and the first sample is injected into the quantification ring through the first six-way valve; The pump body in fluid communication with the first six-way valve is started to transport the mobile phase to flush the quantification ring, so that the second sample is enriched in the second enrichment column; The pump body in fluid communication with the third six-way valve is started to transport the eluent to elute the second enrichment column, and the first sample is injected into the quantification ring through the first six-way valve; The above steps are repeated in a cycle.

2. The method of claim 1, wherein the online enrichment processing system is applied to, A quantification ring is arranged between the two interfaces of the first six-way valve; When the first six-way valve is switched to the first state, the second six-way valve is correspondingly in the first position, and the third six-way valve is correspondingly in the third position, the sample is injected into the quantification ring; When the first six-way valve is switched to the second state, the second six-way valve is correspondingly in the second position, and the third six-way valve is correspondingly in the fourth position, the pump body in fluid communication with the first six-way valve transports the mobile phase to flush the quantification ring, so that the sample is enriched in the first enrichment column; When the first six-way valve is switched to the first state, the second six-way valve is correspondingly in the first position, and the third six-way valve is correspondingly in the third position, the pump body in fluid communication with the third six-way valve transports the eluent to elute the first enrichment column for online determination, and the second sample is injected into the quantification ring; When the first six-way valve switches to the second state, the second six-way valve is correspondingly located at the first position, and the third six-way valve is correspondingly located at the third position, the pump body in fluid communication with the first six-way valve transports flow to flush the dosing loop, and the second sample is enriched to the second enrichment column; When the first six-way valve remains unchanged, the second six-way valve is correspondingly located at the second position, and the third six-way valve is correspondingly located at the fourth position, the pump body in fluid communication with the first six-way valve transports flow to enrich the first sample to the first enrichment column, and the pump body in fluid communication with the third six-way valve transports eluent to elute the second enrichment column for online determination.

3. The method of claim 2, wherein the online enrichment processing system is applied to, The 1# interface of the first six-way valve is connected with a sample injection device, and the 2# interface is connected with waste liquid; When the first six-way valve switches to the first state, the 6# interface of the first six-way valve is in communication with the 1# interface, the 3# interface is in communication with the 2# interface, the dosing loop is arranged between the 3# interface and the 6# interface, the 5# interface is in communication with one of the pump bodies, and the 4# interface is in communication with the second six-way valve; When the first six-way valve switches to the second state, the 6# interface of the first six-way valve is in communication with the 5# interface, the 3# interface is in communication with the 4# interface, the 1# interface is in communication with the 2# interface, the dosing loop is arranged between the 3# interface and the 6# interface, the 5# interface is in communication with one of the pump bodies, and the 4# interface is in communication with the second six-way valve.

4. The method of claim 2, wherein the online enrichment processing system is applied to, The 5# interface of the second six-way valve is in communication with the first six-way valve, and the 2# interface is connected with a detection device; When the second six-way valve is located at the first position, the 6# interface of the second six-way valve is in communication with the 5# interface, the 4# interface is in communication with the 6# interface, the 3# interface is in communication with the 4# interface, the 2# interface is in communication with the 1# interface, the inlet end of the second enrichment column is in communication with the 3# interface of the second six-way valve, the outlet end of the second enrichment column is in communication with the third six-way valve, the 1# interface is in communication with the outlet end of the first enrichment column, and the 2# interface is in communication with the 1# interface; When the second six-way valve is located at the second position, the 1# interface of the second six-way valve is in communication with the 6# interface, the 6# interface is in communication with the 4# interface, the 4# interface is in communication with the 5# interface, the 2# interface is in communication with the 3# interface, the outlet end of the second enrichment column is in communication with the 3# interface of the second six-way valve, the inlet end of the second enrichment column is in communication with the third six-way valve, and the 1# interface is in communication with the inlet end of the first enrichment column.

5. The method of claim 2, wherein the online enrichment processing system is applied to, The 2# interface of the third six-way valve is in communication with one of the pump bodies, and the 5# interface is connected with waste liquid; When the third six-way valve is located at the third position, the 1# interface of the third six-way valve is in communication with the 2# interface, the 3# interface is in communication with the 4# interface, the 4# interface is in communication with the 6# interface, the 6# interface is in communication with the 5# interface, the outlet end of the second enrichment column is in communication with the 3# interface, and the inlet end of the first enrichment column is in communication with the 1# interface; When the third six-way valve is located at the fourth position, the 2# interface of the third six-way valve is in communication with the 3# interface, the 1# interface is in communication with the 6# interface, the 6# interface is in communication with the 4# interface, the 4# interface is in communication with the 5# interface, the inlet end of the second enrichment column is in communication with the 3# interface, and the outlet end of the first enrichment column is in communication with the 1# interface.

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