Rapid loading device

Abstract

The present invention discloses a rapid sample adding device, which belongs to the field of medical devices and includes a sample plate and a receiving plate. The sample plate is provided with a sample receiving portion and a plurality of liquid introduction needles located in the sample receiving portion. The receiving plate includes a main body, a first sealing film and a second sealing film. The main body is provided with a plurality of receiving holes and an injection flow channel. The injection flow channel is connected to the receiving hole. The first sealing film seals the end of the receiving hole, and the second sealing film seals the injection flow channel, so that a negative pressure is formed in the receiving hole and the injection flow channel connected to the receiving hole. When the rapid sample adding device is used, the receiving plate is installed on the sample plate, and the liquid introduction needle pierces the second sealing film so that the liquid sample in the sample receiving portion flows into each receiving hole along the injection flow channel respectively. Through the above design, samples can be added to multiple receiving holes at one time and the mutual mixing and contamination of the solutions in each receiving hole can be avoided.

Description

Technical Field

[0001] The present invention relates to the field of medical devices, in particular to a rapid sample adding device. Background Art

[0002] In the biomedical field, it's common to need to add a sample to every well of a multi-well plate while preventing cross-contamination between wells. Existing technology uses disposable pipettes, which require replacing disposable pipette tips between well additions. This is not only time-consuming and labor-intensive, but also increases disposable tip consumption. Summary of the Invention

[0003] In order to overcome the deficiencies of the prior art, one of the objectives of the present invention is to provide a rapid sample adding device that can add samples to multiple receiving wells at one time and avoids the mixing and contamination of solutions in the various receiving wells.

[0004] One of the purposes of the present invention is achieved by the following technical solution:

[0005] A rapid loading device comprises a sample plate and a receiving plate, wherein the sample plate is provided with a sample receiving portion and a plurality of liquid introduction needles located in the sample receiving portion, the receiving plate comprises a main body, a first sealing film and a second sealing film, the main body is provided with a plurality of receiving holes and an injection flow channel, the injection flow channel is connected to the receiving hole, the first sealing film seals the end of the receiving hole, and the second sealing film seals the injection flow channel, so that a negative pressure is formed in the receiving hole and the injection flow channel connected to the receiving hole. When the rapid loading device is in use, the receiving plate is mounted on the sample plate, and the liquid introduction needle pierces the second sealing film so that the liquid sample in the sample receiving portion flows into each of the receiving holes along the injection flow channel.

[0006] Furthermore, the number of the injection channels is the same as the number of the accommodating holes, each of the injection channels extends from the bottom of the outer wall of the accommodating hole to near the top, and the injection channel is connected to the inside of the accommodating hole near the top of the accommodating hole.

[0007] Furthermore, the inner wall of the accommodating hole is provided with a protrusion and a recess, the protrusion and the recess are correspondingly arranged and combined to form an inlet flow channel, and the top of the inlet flow channel is connected to the accommodating hole.

[0008] Furthermore, the accommodating hole is a hollow structure, the accommodating hole includes a bottom surface, the sampling flow channel extends to the bottom surface and forms a through hole on the bottom surface, and the second sealing film seals the through hole.

[0009] Furthermore, the liquid introduction needle is a hollow structure.

[0010] Furthermore, the liquid introduction needle is provided with a notch, the notch extends along the extension direction of the liquid introduction needle, and the interior of the liquid introduction needle is communicated with the sample receiving portion through the notch.

[0011] Furthermore, the end of the liquid introduction needle is tapered.

[0012] Furthermore, the sample plate is further provided with a sample adding groove, a liquid inlet, an overflow port and a waste liquid groove. The sample adding groove is connected with the sample receiving portion through the liquid inlet, and the sample receiving portion is connected with the waste liquid groove through the overflow port.

[0013] Furthermore, the bottom of the sample adding tank is tilted, and the lowest point of the bottom of the sample tank is connected to the bottom of the liquid inlet.

[0014] Furthermore, the sample receiving portion is a plurality of sample holes, and the plurality of sample holes are connected through a main channel.

[0015] Compared with the prior art, the sample plate of the rapid loading device of the present invention is provided with a sample receiving portion and a plurality of liquid introduction needles located in the sample receiving portion. The receiving plate includes a main body, a first sealing film and a second sealing film. The main body is provided with a plurality of receiving holes and an injection flow channel. The injection flow channel is connected to the receiving hole. The first sealing film seals the end of the receiving hole, and the second sealing film seals the injection flow channel, so that a negative pressure is formed in the receiving hole and the injection flow channel connected to the receiving hole. When the rapid loading device is in use, the receiving plate is installed on the sample plate, and the liquid introduction needle pierces the second sealing film so that the liquid sample in the sample receiving portion flows into each receiving hole along the injection flow channel respectively. Through the above design, multiple receiving holes can be sampled at one time and the mutual mixing and contamination of the solutions in each receiving hole can be avoided. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 A three-dimensional diagram of the rapid sample loading device of the present invention;

[0017] Figure 2 for Figure 1 Exploded view of the rapid sample loading device;

[0018] Figure 3 for Figure 2 A three-dimensional diagram of a sample plate of a rapid sample loading device;

[0019] Figure 4 for Figure 3 A cross-sectional view of a sample plate;

[0020] Figure 5 for Figure 2 An exploded view of a receiving plate of a rapid loading device;

[0021] Figure 6 for Figure 5 A perspective view of the main body of the receiving plate;

[0022] Figure 7 for Figure 6 An enlarged view of point A of the subject;

[0023] Figure 8 for Figure 6 Another stereoscopic view of the subject;

[0024] Figure 9 for Figure 1 A cross-sectional view of a rapid sample loading device;

[0025] Figure 10 for Figure 9 An enlarged view of point B of the rapid sample loading device.

[0026] In the figure: 11, sample plate; 111, sample adding groove; 112, liquid inlet; 113, main channel; 114, sample receiving part; 115, overflow port; 116, waste liquid tank; 117, first sealing groove; 118, liquid introduction needle; 12, receiving plate; 120, main body; 1201, receiving hole; 12010, inner wall; 12011, protrusion; 12012, top; 12013, depression; 12014, bottom; 12015, through hole; 1202, sample injection channel; 1203, sample adding port; 1204, first end face; 1205, second end face; 1206, second sealing groove; 121, first sealing membrane; 122, second sealing membrane; 13, sealing ring. DETAILED DESCRIPTION

[0027] The following will clearly and completely describe the technical solutions in the embodiments of the present invention in conjunction with the accompanying drawings. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0028] It should be noted that when a component is referred to as being "fixed to" another component, it may be directly on the other component or there may be another intermediate component through which it is fixed. When a component is considered to be "connected to" another component, it may be directly connected to the other component or there may be another intermediate component at the same time. When a component is considered to be "set on" another component, it may be directly set on the other component or there may be another intermediate component at the same time. The terms "vertical", "horizontal", "left", "right" and similar expressions used herein are for illustrative purposes only.

[0029] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by those skilled in the art to which this invention pertains. The terms used in this specification of the present invention are for the purpose of describing specific embodiments only and are not intended to limit the present invention. The term "and / or" as used herein includes any and all combinations of one or more of the associated listed items.

[0030] like Figures 1 to 10 As shown, the rapid sample loading device of the present invention includes a sample plate 11 , a receiving plate 12 and a sealing ring 13 .

[0031] The sample plate 11 is provided with a sample loading slot 111 , a liquid inlet 112 , a main channel 113 , a sample receiving portion 114 , an overflow port 115 , a waste liquid tank 116 , a first sealing slot 117 and a liquid guide needle 118 .

[0032] The sample loading slot 111 is located at the end of the sample plate 11 . In this embodiment, the sample loading slot 111 is in an elongated strip shape, and the bottom of the sample loading slot 111 is an inclined surface, and the lowest point of the inclined surface is connected to the liquid inlet 112 .

[0033] The liquid inlet 112 is located between the sample loading groove 111 and the sample holding portion 114. The solution sample enters the sample holding portion 114 through the sample loading groove 111 and the liquid inlet 112. In this embodiment, the sample holding portion 114 comprises a plurality of cylindrical holes, interconnected by a main channel 113. This design allows only a small amount of solution sample to fill the sample holding portion 114. In other embodiments, the sample holding portion 114 may also be a rectangular recess.

[0034] The overflow port 115 is located at the other end of the sample receiving portion 114 . When too much sample solution is added, the sample solution overflows from the overflow port 115 to the waste liquid tank 116 , thereby maintaining the high uniformity of the sample solution.

[0035] The first sealing groove 117 is used to install the sealing ring 13 , which seals the sample plate 11 and the receiving plate 12 to prevent the sample solution in the sample plate 11 from overflowing through the contact surface.

[0036] There are multiple liquid introduction needles 118. Specifically, the number of liquid introduction needles 118 is equal to the number of receiving holes 1201, so that each liquid introduction needle 118 corresponds to a receiving hole 1201. The liquid introduction needle 118 has a hollow structure and is provided with a notch extending along the extension direction of the liquid introduction needle 118 (in the vertical direction). The interior of the liquid introduction needle 118 is connected to the sample receiving portion 114 through the notch. The end of the liquid introduction needle 118 is tapered to facilitate the liquid introduction needle 118 to pierce the second sealing membrane 122.

[0037] The receiving plate 12 includes a main body 120 , a first sealing film 121 , and a second sealing film 122 .

[0038] The main body 120 is provided with a plurality of accommodating holes 1201 , a plurality of injection channels 1202 , a sample addition port 1203 , a first end surface 1204 , a second end surface 1205 and a second sealing groove 1206 .

[0039] A plurality of receiving holes 1201 are disposed on the first end surface 1204 . Each receiving hole 1201 extends downward from the first end surface 1204 and has a hollow structure.

[0040] The number of inlet channels 1202 is the same as the number of receiving holes 1201. Each inlet channel 1202 extends from the bottom of the outer wall of the receiving hole 1201 to near the top of the receiving hole 1201. The inlet channel 1202 communicates with the interior of the receiving hole 1201 near the top of the receiving hole 1201. Specifically, the inner wall 12010 of the receiving hole 1201 is provided with a protrusion 12011 and a depression 12013. The protrusion 12011 and the depression 12013 are correspondingly arranged and closed to form the inlet channel 1202. The top 12012 of the inlet channel 1202 communicates with the receiving hole 1201. The receiving hole 1201 includes a bottom surface 12014. The inlet channel 1202 extends to the bottom surface 12014 and forms a through hole 12015 in the bottom surface 12014. The second sealing membrane 122 seals the through hole 12015.

[0041] The sample loading port 1203 is located at the end of the receiving plate 12 and is used to pour the sample solution into the loading channel 111. The first end surface 1204 is lower than the second end surface 1205, facilitating the installation of the first sealing membrane 121 against the first end surface 1204, thereby achieving a tight seal on the receiving hole 1201. The second sealing groove 1206 is used to mount the sealing ring 13, which seals the sample plate 11 and the receiving plate 12, preventing the sample solution in the sample plate 11 from spilling out through the contact surface.

[0042] When the rapid sample loading device of the present invention is used, in the initial state, the sample plate 11 is separated from the receiving plate 12 .

[0043] Each receiving hole 1201 of the receiving plate 12 is filled with drugs of varying types and concentrations. The sample inlet channel 1202 is the sole channel for the sample solution to enter the receiving hole 1201 from the sample holding portion 114. The through hole 12015 at the lower end of the sample inlet channel 1202 is sealed by a second sealing film 122. The first end face 1204 is lower than the second end face 1205, facilitating the installation of the first sealing film 121 against the first end face 1204, thereby achieving a tight seal within the receiving hole 1201. The first sealing film 121 and the second sealing film 122 cooperate to maintain a complete seal within the receiving hole 1201, creating a negative pressure environment with a certain degree of vacuum. By controlling the vacuum level within the receiving hole 1201, the amount of sample solution drawn in is controlled, facilitating a more uniform and precise transfer of the sample solution.

[0044] The sample solution is poured into the sample injection tank 111 , flows into the main channel 113 through the liquid inlet 112 , and is evenly distributed to the sample receiving portion 114 through the main channel 113 . The sample solution is at least higher than the through hole 12015 of the sample injection channel 1202 . Excess sample solution flows into the waste liquid tank 116 through the overflow port 115 .

[0045] Receiving plate 12 is installed on sample plate 11. During installation, receiving plate 12 presses downward against sample plate 11, and sealing ring 13 seals sample plate 11 and receiving plate 12, preventing the sample solution in sample plate 11 from overflowing through the contact surface. Simultaneously, liquid introduction needle 118 pierces second sealing membrane 122. Under the influence of the negative pressure in receiving hole 1201, the sample solution follows liquid introduction needle 118 through injection channel 1202 into receiving hole 1201, mixing with the drug powder to form a mixed solution. By controlling the vacuum level in receiving hole 1201, the amount of sample solution drawn in is controlled, facilitating even and precise transfer of the sample solution.

[0046] In the present application, the vacuum degree in the receiving hole 1201 is controlled to achieve accurate transfer of the sample solution from the sample plate 11 to the receiving plate 12, eliminating the tedious process of pipetting liquid one by one in the traditional combination plate, and avoiding the waste caused by replacing disposable pipette tips.

[0047] The above embodiments merely illustrate several implementations of the present invention, and while their descriptions are relatively specific and detailed, they should not be construed as limiting the scope of the patented invention. It should be noted that those skilled in the art would be able to make numerous variations and improvements without departing from the spirit of the present invention. These variations and improvements are equivalent modifications and improvements to the above embodiments based on the essential technology of the present invention and fall within the scope of protection of the present invention.

Claims

1. A rapid sample adding device, characterized in that: The invention comprises a sample plate and a receiving plate, wherein the sample plate is provided with a sample loading groove, a sample receiving portion and a plurality of liquid introduction needles located in the sample receiving portion, the sample receiving portion being a plurality of sample wells, the plurality of sample wells being connected through a main channel, the sample loading groove being connected to the plurality of sample wells, and liquid samples being added to the plurality of sample wells at one time through the sample loading groove, the receiving plate comprising a main body, a first sealing film and a second sealing film, the main body being provided with a plurality of receiving holes and an injection flow channel, the inner wall of the receiving hole being provided with a protrusion and a recess, the protrusion and the recess being arranged correspondingly and closing to form the injection flow channel, the top of the injection flow channel being connected to the receiving hole, the first sealing film sealing the end of the receiving hole, and the second sealing film sealing the injection flow channel, so that a negative pressure is formed in the receiving hole and the injection flow channel connected to the receiving hole, when the rapid loading device is in use, the receiving plate is mounted on the sample plate, the liquid introduction needle pierces the second sealing film so that the liquid sample in the sample receiving portion flows into each of the receiving holes along the injection flow channel respectively.

2. The rapid sample adding device according to claim 1, characterized in that: The number of the injection channels is the same as the number of the accommodating holes. Each of the injection channels extends from the bottom of the outer wall of the accommodating hole to near the top, and the injection channel is connected to the inside of the accommodating hole near the top.

3. The rapid sample adding device according to claim 1, characterized in that: The accommodating hole is a hollow structure and includes a bottom surface. The sampling flow channel extends to the bottom surface and forms a through hole on the bottom surface. The second sealing film seals the through hole.

4. The rapid sample adding device according to claim 1, characterized in that: The liquid introduction needle is a hollow structure.

5. The rapid sample adding device according to claim 4, characterized in that: The liquid introduction needle is provided with a notch, and the notch extends along the extension direction of the liquid introduction needle. The interior of the liquid introduction needle is communicated with the sample receiving portion through the notch.

6. The rapid sample adding device according to claim 4, characterized in that: The end of the liquid guide needle is tapered.

7. The rapid sample loading device according to claim 1, characterized in that: The sample plate is further provided with a liquid inlet, an overflow port and a waste liquid tank. The sample adding tank is communicated with the sample receiving portion through the liquid inlet, and the sample receiving portion is communicated with the waste liquid tank through the overflow port.

8. The rapid sample adding device according to claim 7, characterized in that: The bottom of the sample adding groove is arranged obliquely, and the lowest point of the bottom of the sample adding groove is connected to the bottom of the liquid inlet.

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