A tissue culture room sterilization device, system and method

Abstract

The present invention discloses a tissue culture room sterilization device, system and method, the device includes a chassis, a gas collection area, a disinfection reaction area, an electrical control area and a fresh air area are provided in the chassis, the gas collection area is arranged between the fresh air area and the disinfection reaction area, a built-in fan is provided in the gas collection area, a circulating air inlet is provided near the gas collection area on the chassis, a fresh air inlet is provided in the fresh air area, the disinfection reaction area is provided with a reaction module, an air outlet is provided near the reaction module on the chassis, the air outlet is provided with an ozone detector, a narrow pulse power supply and a stand-alone controller are provided in the electrical control area, the stand-alone controller is electrically connected to the ozone detector and the narrow pulse power supply respectively, the stand-alone controller is electrically connected to the built-in fan via a frequency converter, and the output end of the narrow pulse power supply is connected to the reaction module. The present invention has multiple operating modes, and the reaction module ensures that the air is disinfected and disinfected efficiently. At the same time, this disinfection method will not harm the human body, and the device operates stably and reliably.

Description

Technical Field

[0001] The present invention relates to the technical field of indoor sterilization, and in particular to a tissue culture room sterilization device, system and method. Background Art

[0002] A tissue culture room is a small, controllable environmental chamber that can meet a variety of experimental needs. This allows for the flexible adjustment of the climate for plant growth within a certain range, eliminating natural interference and seasonal restrictions on experimental work. This significantly shortens experimental research cycles and improves accuracy and efficiency. Tissue culture rooms generally consist of five compartments: a buffer room, a preparation room, a sterile room, a culture room, and a seedling hardening room. Because the temperature in a tissue culture room is ideal for bacterial growth, air disinfection is necessary to prevent bacterial infection and rot in tissue culture seedlings.

[0003] Maintaining the cleanliness of the air in the tissue culture room is an important condition for reducing tissue culture contamination. Some common methods currently used for laboratory disinfection include ultraviolet irradiation, formaldehyde fumigation, and chlorhexidine (benzalkonium bromide solution).

[0004] Ultraviolet irradiation disinfection uses no chemical agents and produces no harmful byproducts to humans or the environment. However, it should be noted that excessive ultraviolet radiation can be harmful to the human body and may even cause cancer. Therefore, while the tissue culture room is being disinfected with ultraviolet light, staff should not remain in the room. After disinfection, staff should wait 15-20 minutes after turning off the UV lamp before reentering.

[0005] Formaldehyde fumigation is a harmful substance to the human body. Direct skin contact with formaldehyde can cause allergic dermatitis and pigmentation. Inhalation of high concentrations of formaldehyde can induce allergic asthma. High concentrations of formaldehyde are also genotoxic, and inhaling high concentrations in laboratory animals can cause nasopharyngeal tumors. Long-term breathing of air containing high concentrations of formaldehyde can also cause menstrual disorders in women, affect fertility, and cause physical decline and chromosomal abnormalities in newborns. In particular, when indoor formaldehyde levels exceed the standard, it can cause leukemia and lead to death.

[0006] Sanisol (benzalkonium bromide solution) method. Basically, Sanisol is less toxic to the human body, but it should be noted that repeated or long-term exposure may cause damage to the cardiovascular system, digestive system, and especially the reproductive system, and may also cause allergic reactions.

[0007] Existing disinfection methods have certain drawbacks to varying degrees, which will more or less cause certain harm to people, increase time costs, and lead to low sterilization efficiency. Summary of the Invention

[0008] In order to solve the above problems, the technical solution provided by the present invention is:

[0009] A tissue culture room sterilization device includes a chassis, wherein a gas collection area, a disinfection reaction area, an electrical control area and a fresh air area are provided in the chassis, the gas collection area is arranged between the fresh air area and the disinfection reaction area, and a built-in fan is provided in the gas collection area. A circulating air inlet is provided on the chassis near the gas collection area, a fresh air inlet is provided in the fresh air area, a reaction module is provided in the disinfection reaction area, an air outlet is provided on the chassis near the reaction module, an ozone detector is provided at the air outlet, a narrow pulse power supply and a stand-alone controller are provided in the electrical control area, the stand-alone controller is electrically connected to the ozone detector and the narrow pulse power supply respectively, the stand-alone controller is electrically connected to the built-in fan through a frequency converter, and the output end of the narrow pulse power supply is connected to the reaction module.

[0010] The present invention is further configured such that a module bracket is provided in the disinfection reaction area, and the reaction module includes a first fixed plate and a second fixed plate, the first fixed plate and the second fixed plate are connected to the module bracket, a wind shield is provided on the periphery of the first fixed plate or the periphery of the second fixed plate, an array-arranged discharge tube is provided between the first fixed plate and the second fixed plate, a first positioning bracket is provided on one side of the first fixed plate, and a second positioning bracket is provided on one side of the second fixed plate, a discharge electrode is provided between the discharge tubes, the two ends of the discharge electrode are respectively connected to the first positioning bracket and the second positioning bracket, and the output end of the narrow pulse power supply is connected to the discharge electrode.

[0011] The present invention is further configured such that the discharge electrode includes a pole wire body, a plurality of discharge plates are provided on the pole wire body, an intermediate clamping barrel is provided between the discharge plates, and outer clamping barrels are provided at both ends of the pole wire body, and the upper and lower end faces of the intermediate clamping barrel are provided with spaced positioning grooves and positioning blocks, the positioning grooves and positioning blocks on the upper end face of the intermediate clamping barrel are staggered with the positioning grooves and positioning blocks on the lower end face of the same intermediate clamping barrel, the end face of the outer clamping barrel close to the discharge plate is provided with spaced positioning grooves and positioning blocks, the outer periphery of the discharge plate is evenly provided with discharge ends, the discharge end limit is located between the positioning grooves and positioning blocks adjacent to the discharge end, and the discharge end protrudes from the surface of the intermediate clamping barrel and the surface of the outer clamping barrel, and the discharge ends of adjacent discharge plates are staggered along the axial direction of the pole wire body.

[0012] The present invention is further configured to provide a flow balancing plate between the disinfection reaction zone and the gas collection zone, and the flow balancing plate is provided with a plurality of flow balancing holes.

[0013] The present invention is further configured such that the fresh air zone is provided with a fresh air outlet flange, and the fresh air outlet flange is provided with a fresh air outlet adapter.

[0014] The present invention is further configured such that a fan bracket is provided in the gas collection area, and the built-in fan is fixed on the fan bracket.

[0015] The present invention is further configured as a power supply bracket in the electrical control area, wherein a control box is provided on the power supply bracket, and the narrow pulse power supply is located in the control box.

[0016] A tissue culture room sterilization system includes a main controller, a fresh air blower and at least one chamber, wherein the above-mentioned tissue culture room sterilization device is arranged in the chamber, the air inlet side of the fresh air blower is connected to the external air, and the air outlet side of the fresh air blower is connected to the fresh air main duct, the fresh air main duct is connected to the fresh air inlet through a fresh air branch duct, and the fresh air branch duct is provided with a solenoid valve for controlling the conduction and closing of the fresh air branch duct, the main controller is communicatively connected to the stand-alone controller, and the main controller is electrically connected to the fresh air blower and the solenoid valve respectively.

[0017] The present invention is further configured to include a communication module and a control panel. The master controller is connected to the cloud backend through the communication module, and the control panel is electrically connected to the master controller.

[0018] A tissue culture room sterilization method, used in the above-mentioned tissue culture room sterilization system, comprising:

[0019] In the low ozone operation mode, the main controller controls the solenoid valve corresponding to the compartment to be closed; the stand-alone controller controls the operation of the built-in fan and the narrow pulse power supply. The narrow pulse power supply operates within the first output power range. The built-in fan draws in the air in the compartment from the circulating air inlet. The inhaled air passes through the flow equalizing plate to evenly distribute the airflow and send it into the disinfection reaction area. The air after equalization passes through the discharge electrode. The high-energy electrons generated by the discharge electrode bombard the oxygen and water vapor in the gas, which are activated, decomposed and ionized to produce free radicals, atomic oxygen and ozone with strong oxidizing ability. The free radicals, atomic oxygen and ozone with strong oxidizing ability undergo sufficient redox reactions with the microorganisms in the inhaled air to complete the air purification. The purified air is discharged back into the compartment from the air outlet; the ozone detector detects the ozone concentration in the air outlet in real time. The stand-alone controller adjusts the output power of the narrow pulse power supply within the first output power range according to the ozone concentration feedback from the ozone detector, and controls the ozone concentration at the air outlet to be within the first ozone threshold.

[0020] In high ozone operation mode, the main controller controls the solenoid valve corresponding to the compartment to be closed; the stand-alone controller controls the operation of the built-in fan and the narrow pulse power supply. The narrow pulse power supply operates in the second output power range, and the built-in fan draws the air in the compartment from the circulating air inlet. The inhaled air passes through the flow equalizing plate to evenly distribute the airflow and send it into the disinfection reaction area. The air after equalization passes through the discharge electrode. The high-energy electrons generated by the discharge electrode bombard the oxygen and water vapor in the gas, and after activation, decomposition and ionization, free radicals, atomic oxygen and ozone with strong oxidizing ability are generated. The free radicals, atomic oxygen and ozone with strong oxidizing ability react with the inhaled air. The microorganisms in the air undergo a sufficient redox reaction, and the purified air is enriched with high-concentration ozone and is re-discharged from the air outlet into the compartment to disinfect the microorganisms in the compartment; the ozone detector detects the ozone concentration in the air outlet in real time, and the stand-alone controller adjusts the output power of the narrow pulse power supply within the second output power range according to the ozone concentration fed back by the ozone detector, and controls the ozone concentration at the air outlet to be within the second ozone threshold; after the disinfection is completed, the stand-alone controller controls the operation within the third output power range, and the ozone passing through the discharge electrode is electrolyzed into oxygen, so that the ozone concentration in the compartment is quickly reduced to zero;

[0021] In the fresh air operation mode, the main controller controls the solenoid valve corresponding to the compartment to open, and controls the operation of the fresh air fan at the same time; the stand-alone controller controls the operation of the built-in fan and the narrow pulse power supply. The narrow pulse power supply operates within the first output power range, and the fresh air fan sends external air into the fresh air inlet through the fresh air main duct and the fresh air branch duct. The inhaled air passes through the flow equalizing plate to evenly distribute the airflow and is sent into the disinfection reaction area. The evenly distributed air passes through the discharge electrode. The high-energy electrons generated by the discharge electrode bombard the oxygen and water vapor in the gas, and after activation, decomposition and ionization, they produce free radicals, atomic oxygen and ozone with strong oxidizing ability. The free radicals, atomic oxygen and ozone with strong oxidizing ability undergo sufficient redox reactions with the microorganisms in the inhaled air to complete the air purification. The purified air is discharged from the air outlet into the compartment; the ozone detector detects the ozone concentration in the air outlet in real time. The stand-alone controller adjusts the output power of the narrow pulse power supply within the first output power range according to the ozone concentration feedback from the ozone detector, and controls the ozone concentration at the air outlet to be within the first ozone threshold.

[0022] Compared with the prior art, the technical solution provided by the present invention has the following beneficial effects:

[0023] The tissue culture chamber sterilization device of the present invention incorporates a narrow pulse power supply and a reaction module. The narrow pulse power supply causes the reaction module to generate high-energy electrons under high-voltage discharge, bombarding and disinfecting gas molecules (O2 and H2O) in the reaction zone. Through activation, decomposition, and ionization, these highly oxidizing free radicals ("OH," "O"), atomic oxygen (O), and ozone (O3) are generated. These strong oxidizing substances undergo sufficient redox reactions with the gas, ultimately achieving the goal of purifying bacterial oxidation. A controller adjusts the output power of the narrow pulse power supply to vary the concentration of oxidizing substances, such as ozone, produced by the reaction module, enabling the device to operate in different modes according to different requirements. The high energy intensity and density of the pulsed plasma reaction generated by the reaction module within the disinfection reaction module ensures efficient disinfection. This disinfection method is harmless to the human body, and the device operates stably and reliably.

[0024] The tissue culture room sterilization device of the present invention is installed in the compartment of the laboratory. It can not only perform daily purification of the compartment in low-ozone operation and deep disinfection in high-ozone operation in an internal circulation manner, but also can be connected to the fresh air system. When the external air enters the compartment, it is sterilized by the device and then the fresh air is discharged into the compartment, thereby realizing the circulation and cleaning of the compartment air.

[0025] The reaction module of the present invention adopts an array discharge tube and a built-in discharge electrode structure. In order to improve the rigidity of the discharge electrode and solve the energy loss problem, a barrel-type structure is used to position the discharge plate, thereby improving the connection strength of the discharge plate and making the discharge end less likely to bend and capable of stable discharge. At the same time, the barrel-type structure can make the gas flow through the discharge electrode more smoothly, reducing the energy loss caused by the collision between the airflow and the discharge plate, and allowing the gas to almost completely pass through the pulse plasma area, thereby improving the gas processing efficiency; the staggered discharge end makes the discharge point arrangement density on the discharge cross section in the discharge tube higher, and the pulse plasma area can cover more areas through which the gas flows.

[0026] The tissue culture room sterilization method of the present invention includes a low ozone operation mode, a high ozone operation mode and a fresh air operation mode, can be operated in corresponding modes according to the needs of the laboratory, has full automatic control, is flexible to use, and ensures that the indoor environment of the area is clean. BRIEF DESCRIPTION OF THE DRAWINGS

[0027] Figure 1 This is a schematic diagram of the internal structure of the tissue culture room sterilization device according to an embodiment of the present invention.

[0028] Figure 2 This is an exploded view of the tissue culture room sterilization device according to an embodiment of the present invention.

[0029] Figure 3 This is a partially enlarged view of the reaction module of an embodiment of the present invention.

[0030] Figure 4 This is a three-dimensional diagram of the discharge electrode according to an embodiment of the present invention.

[0031] Figure 5 This is a partial exploded view of the discharge electrode according to an embodiment of the present invention.

[0032] Figure 6 Schematic diagram of discharge of discharge electrodes when airflow passes through a discharge tube according to an embodiment of the present invention.

[0033] Figure 7 Schematic diagram of discharge cross section of discharge electrode wire according to an embodiment of the present invention.

[0034] Figure 8 This is an electrical control diagram of the tissue culture room sterilization device according to an embodiment of the present invention.

[0035] Figure 9 Schematic diagram of a tissue culture room sterilization system according to an embodiment of the present invention.

[0036] Figure 10 This is an electrical control diagram of the tissue culture room sterilization system in an embodiment of the present invention. DETAILED DESCRIPTION

[0037] In order to further understand the content of the present invention, the present invention is described in detail with reference to the accompanying drawings and embodiments.

[0038] It should be noted that, in the absence of conflict, the embodiments of the present invention and the features therein may be combined with each other.

[0039] In the description of the present invention, it should be noted that, unless otherwise clearly specified and limited, the terms "installed", "connected" and "connected" should be understood in a broad sense. For example, it can be a fixed connection, an integral connection, or a detachable connection; it can be a mechanical connection or an electrical connection, or it can be the internal communication between two components; it can be a direct connection or an indirect connection through an intermediate medium. For ordinary technicians in this field, the specific meanings of the above terms can be understood according to the specific circumstances.

[0040] Example 1

[0041] Combined with attachment Figure 1 To the attached Figure 8The technical solution of the present invention is a tissue culture room sterilization device, comprising a chassis 1, wherein the chassis 1 is provided with a gas collection area 11, a disinfection reaction area 12, an electrical control area 13 and a fresh air area 14, the gas collection area 11 is arranged between the fresh air area 14 and the disinfection reaction area 12, and a built-in fan 2 is provided in the gas collection area 11, a circulating air inlet 15 is provided on the chassis 1 near the gas collection area 11, the fresh air area 14 is provided with a fresh air inlet 16, the disinfection reaction area 12 is provided with a reaction module 3, an air outlet 17 is provided on the chassis 1 near the reaction module 3, and an ozone detector 4 is provided at the air outlet 17. A narrow pulse power supply 5 and a stand-alone controller 6 are provided in the electrical control area 13, and the stand-alone controller 6 is electrically connected to the ozone detector 4 and the narrow pulse power supply 5 respectively. The stand-alone controller 6 is electrically connected to the built-in fan 2 through a frequency converter 21, and the output end of the narrow pulse power supply 5 is connected to the reaction module 3.

[0042] In the above embodiment, the built-in fan 2 is the power source for the device to circulate air. There are two ways to circulate gas in the gas collection area 11. One is the indoor circulating air entering from the circulating air inlet 15, and the other is the external circulating air entering from the fresh air inlet 16.

[0043] In the above embodiment, the stand-alone controller 6 is a programmable logic controller PLC; the stand-alone controller 6 adjusts the frequency of the inverter 21 according to the size of different compartments to adjust the air suction volume per unit time of the built-in fan 2; the ozone detector 4 monitors the ozone concentration of the air discharged from the air outlet 17, and the output signal of the ozone detector 4 is sent to the stand-alone controller 6; the stand-alone controller 6 adjusts the output power of the narrow pulse power supply 5 to realize stepless regulation of the narrow pulse power supply 5; the reaction module 3 ionizes the air flowing through the disinfection reaction zone to different degrees according to the different power outputs of the narrow pulse power supply 5.

[0044] In the above embodiment, under the control of the stand-alone controller 6, air purification in a separate compartment can be achieved.

[0045] In this embodiment, a module bracket 121 is provided in the disinfection reaction area 12, and the reaction module 3 includes a first fixed plate 31 and a second fixed plate 32. The first fixed plate 31 and the second fixed plate 32 are connected to the module bracket 121, and a wind shield 33 is provided on the outer periphery of the first fixed plate 31 or the outer periphery of the second fixed plate 32. An array of discharge tubes 34 is provided between the first fixed plate 31 and the second fixed plate 32. A first positioning bracket 35 is provided on one side of the first fixed plate 31, and a second positioning bracket 36 is provided on one side of the second fixed plate 32. A discharge electrode line 37 is provided between the discharge tubes 34, and the two ends of the discharge electrode line 37 are respectively connected to the first positioning bracket 35 and the second positioning bracket 36, and the output end of the narrow pulse power supply 5 is connected to the discharge electrode line 37.

[0046] In the above embodiment, the first positioning bracket 35 and the second positioning bracket 36 are used to position the discharge electrode 37 so that the discharge electrode 37 can be centrally arranged in the corresponding discharge tube 34; the discharge electrode 37 is connected to the output end of the narrow pulse power supply 5 through a busbar or copper wire, thereby obtaining a high voltage to ionize the air.

[0047] In this embodiment, the discharge electrode 37 includes a wire body 371, a plurality of discharge plates 372 are provided on the wire body 371, an intermediate clamping barrel 373 is provided between the discharge plates 372, and an outer clamping barrel 374 is provided at both ends of the wire body 371. The upper and lower end surfaces of the intermediate clamping barrel 373 are provided with positioning grooves 3731 and positioning blocks 3732 arranged at intervals. The positioning grooves 3731 and the positioning blocks 3732 on the upper end surface of the intermediate clamping barrel 373 are aligned with the positioning grooves 3731 and the positioning blocks 3732 on the lower end surface of the same intermediate clamping barrel 373. 32 is staggered, and the outer barrel 374 is provided with spacing positioning grooves 3741 and positioning blocks 3742 near the end face of the discharge plate 372, and the outer periphery of the discharge plate 372 is evenly provided with discharge ends 3721, and the discharge ends 3721 are limited between the positioning grooves and the positioning blocks adjacent to the discharge ends 3721, and the discharge ends 3721 protrude from the surfaces of the intermediate barrel 373 and the outer barrel 374, and the discharge ends 3721 of adjacent discharge plates 372 are staggered along the axial direction of the pole line body 371.

[0048] In the above embodiment, in order to improve the rigidity and energy loss of the discharge electrode line 37, a barrel-type structure is used to position the discharge plate, thereby improving the connection strength of the discharge plate 372, making the discharge end 3721 less prone to bending and able to discharge stably. At the same time, the barrel-type structure can enable the gas to flow through the discharge electrode line 37 more smoothly, reducing the energy loss caused by the collision between the airflow and the discharge plate 372, and allowing the gas to pass almost completely through the pulse plasma area, thereby improving the gas processing efficiency; the staggered discharge end 3721 makes the discharge point arrangement density on the discharge cross section in the discharge tube higher, and the pulse plasma area can cover more areas through which the gas flows.

[0049] In the above embodiment, the pulse plasma area generates pulse plasma. The principle of pulse plasma technology is to use a narrow pulse plasma power box reaction module to generate high-energy electrons (5eV~20eV) under high-voltage discharge to bombard gas molecules (O2 and H2O, etc.) in the reactor; after activation, decomposition and ionization processes, free radicals ("OH", "O"), atomic oxygen (O) and ozone (O3) with strong oxidizing ability are generated. These strong oxidizing substances can undergo sufficient redox reactions with the gas to ultimately achieve the purpose of purifying bacterial oxidation; the main oxidizing substance produced is ozone, so the ozone concentration of the outlet gas is mainly monitored to reflect the degree of ionization in the disinfection reaction zone.

[0050] In this embodiment, a flow balancing plate 111 is provided between the disinfection reaction zone 12 and the gas collection zone 11 , and a plurality of flow balancing holes 112 are provided on the flow balancing plate 111 ; the flow balancing plate 111 enables air to enter each of the discharge tubes 34 in the disinfection reaction zone 12 evenly.

[0051] In this embodiment, the fresh air zone 14 is provided with a fresh air outlet flange 141 , and the fresh air outlet flange 141 is provided with a fresh air outlet adapter 142 .

[0052] In this embodiment, a fan bracket 113 is provided in the gas collection area 11 , and the built-in fan 2 is fixed on the fan bracket 113 .

[0053] In this embodiment, a power supply bracket 131 is provided in the electrical control area 13 , a control box 132 is provided on the power supply bracket 131 , and the narrow pulse power supply 5 is located in the control box 132 .

[0054] The tissue culture room sterilization device of the technical solution of the present invention is installed in the compartment in the laboratory. It can not only perform daily purification of the compartment in low ozone operation and deep disinfection in high ozone operation in an internal circulation, but also can be connected to the fresh air system. When the external air enters the compartment, it is sterilized by the device and the fresh air is discharged into the compartment to achieve circulation and cleaning of the compartment air. The output power of the narrow pulse power supply is adjusted by the controller to change the concentration of oxidizing substances such as ozone generated by the reaction module, so as to realize the operation mode of the device under different requirements. The pulse plasma reaction with higher energy intensity and energy density generated by the reaction module in the disinfection reaction module is a guarantee of high-efficiency disinfection. At the same time, this disinfection method will not cause harm to the human body, and the operation of the device is stable and reliable.

[0055] Example 2

[0056] Combined with attachment Figure 1 To the attached Figure 10 The technical solution of the present invention is a tissue culture chamber sterilization system, comprising a main controller 7, a fresh air blower 8 and at least one compartment 9, each compartment 9 being provided with the tissue culture chamber sterilization device described in Example 1, the air inlet side of the fresh air blower 8 being connected to the outside air, the air outlet side of the fresh air blower 8 being connected to a fresh air main duct 81, the fresh air main duct 81 being connected to the fresh air inlet 16 through a fresh air branch duct 82, the fresh air branch duct 82 being provided with a solenoid valve 83 for controlling the conduction and closing of the fresh air branch duct 82, the main controller 7 being communicatively connected to the stand-alone controller 6, and the main controller 7 being electrically connected to the fresh air blower 8 and the solenoid valve 83 respectively.

[0057] In the above embodiment, the master controller 7 controls the start-up of the fresh air blower 8 and the on / off of the solenoid valve 83 .

[0058] In the above embodiment, the tissue culture chamber sterilization device can be installed on the wall of the chamber 9.

[0059] In the above embodiment, in the tissue culture laboratory, including the culture room, inoculation room, preparation room and changing room, the tissue culture room sterilization device described in Example 1 can be installed in the above rooms and then connected to the fresh air system through the fresh air duct.

[0060] In this embodiment, a communication module 71 and a control panel 72 are further included. The master controller 7 is connected to the cloud backend through the communication module 71 , and the control panel 72 is electrically connected to the master controller 7 .

[0061] In the above embodiment, the staff can select the mode of the tissue culture room sterilization device in the compartment through the control panel 72; the communication module 71 uploads the mode operation data of each compartment to the cloud background for recording and statistics, so as to facilitate notification to the user terminal.

[0062] Example 3

[0063] The technical solution of the present invention is a tissue culture room sterilization method, which is used in the tissue culture room sterilization system described in Example 2, comprising:

[0064] In the low ozone operation mode, the main controller controls the solenoid valve corresponding to the compartment to be closed; the stand-alone controller controls the operation of the built-in fan and the narrow pulse power supply. The narrow pulse power supply operates within the first output power range. The built-in fan draws in the air in the compartment from the circulating air inlet. The inhaled air passes through the flow equalizing plate to evenly distribute the airflow and send it into the disinfection reaction area. The air after equalization passes through the discharge electrode. The high-energy electrons generated by the discharge electrode bombard the oxygen and water vapor in the gas, which are activated, decomposed and ionized to produce free radicals, atomic oxygen and ozone with strong oxidizing ability. The free radicals, atomic oxygen and ozone with strong oxidizing ability undergo sufficient redox reactions with the microorganisms in the inhaled air to complete the air purification. The purified air is discharged back into the compartment from the air outlet; the ozone detector detects the ozone concentration in the air outlet in real time. The stand-alone controller adjusts the output power of the narrow pulse power supply within the first output power range according to the ozone concentration feedback from the ozone detector, and controls the ozone concentration at the air outlet to be within the first ozone threshold.

[0065] In high ozone operation mode, the main controller controls the solenoid valve corresponding to the compartment to be closed; the stand-alone controller controls the operation of the built-in fan and the narrow pulse power supply. The narrow pulse power supply operates in the second output power range, and the built-in fan draws the air in the compartment from the circulating air inlet. The inhaled air passes through the flow equalizing plate to evenly distribute the airflow and send it into the disinfection reaction area. The air after equalization passes through the discharge electrode. The high-energy electrons generated by the discharge electrode bombard the oxygen and water vapor in the gas, and after activation, decomposition and ionization, free radicals, atomic oxygen and ozone with strong oxidizing ability are generated. The free radicals, atomic oxygen and ozone with strong oxidizing ability react with the inhaled air. The microorganisms in the air undergo a sufficient redox reaction, and the purified air is enriched with high-concentration ozone and is re-discharged from the air outlet into the compartment to disinfect the microorganisms in the compartment; the ozone detector detects the ozone concentration in the air outlet in real time, and the stand-alone controller adjusts the output power of the narrow pulse power supply within the second output power range according to the ozone concentration fed back by the ozone detector, and controls the ozone concentration at the air outlet to be within the second ozone threshold; after the disinfection is completed, the stand-alone controller controls the operation within the third output power range, and the ozone passing through the discharge electrode is electrolyzed into oxygen, so that the ozone concentration in the compartment is quickly reduced to zero;

[0066] In the fresh air operation mode, the main controller controls the solenoid valve corresponding to the compartment to open, and controls the operation of the fresh air fan at the same time; the stand-alone controller controls the operation of the built-in fan and the narrow pulse power supply. The narrow pulse power supply operates within the first output power range, and the fresh air fan sends external air into the fresh air inlet through the fresh air main duct and the fresh air branch duct. The inhaled air passes through the flow equalizing plate to evenly distribute the airflow and is sent into the disinfection reaction area. The evenly distributed air passes through the discharge electrode. The high-energy electrons generated by the discharge electrode bombard the oxygen and water vapor in the gas, and after activation, decomposition and ionization, they produce free radicals, atomic oxygen and ozone with strong oxidizing ability. The free radicals, atomic oxygen and ozone with strong oxidizing ability undergo sufficient redox reactions with the microorganisms in the inhaled air to complete the air purification. The purified air is discharged from the air outlet into the compartment; the ozone detector detects the ozone concentration in the air outlet in real time. The stand-alone controller adjusts the output power of the narrow pulse power supply within the first output power range according to the ozone concentration feedback from the ozone detector, and controls the ozone concentration at the air outlet to be within the first ozone threshold.

[0067] Research has shown that ozone sterilization is rapid, occurring almost instantly. Ozone concentrations of 0.5-1ppm can eliminate 80% of naturally occurring bacteria in the air. At concentrations of 6-10ppm, the sterilization rate for bacteria reaches over 90%, and the disinfect rate for mold reaches 80%. Even in low-power mode (no ozone spillage), the ozone concentration within the oxidation reaction zone of this device generally remains around 2ppm. In high-ozone, high-power mode, ozone concentrations can reach as high as 40-50ppm. In summary, the sterilization effect on bacteria and mold is immediate. This concentration far exceeds the required sterilization concentration, and can be controlled in real time, preventing the harmful effects of high ozone concentrations on the human body.

[0068] In the above embodiment, the difference between the low ozone operation mode and the fresh air operation mode lies in the source of the gas. When the narrow pulse power supply operates in the first output power range, the concentration of ozone generated at the air outlet is in the range of 0-0.05ppm. This concentration of ozone will not cause harm to the human body and is safe. The high ozone operation mode is to circulate the air in the zone to increase the concentration of ozone in the zone. When the narrow pulse power supply operates in the second output power range, the concentration of ozone generated at the air outlet is in the range of 0.5-50ppm. The microorganisms on the surface of the equipment in the zone are disinfected. After the disinfection is completed, in order to quickly reduce the ozone concentration in the zone, the narrow pulse power supply is adjusted to the third output power range. At this time, the disinfection reaction zone electrolyzes the ozone in the air to produce oxygen. The reaction mechanism for switching to ozone elimination mode after the high ozone mode ends is as follows: When operating in high ozone elimination mode, the narrow pulse power supply output is high, directly ionizing oxygen in the air to form ozone and other oxygen free radicals. After the elimination is completed, the narrow pulse power supply output is reduced to a level that can ionize ozone but not oxygen. Ozone is then reduced to oxygen, achieving the effect of eliminating ozone. As is well known, ozone is much more active than oxygen, and its bond energy is much lower than that of oxygen, making it more easily ionized. Therefore, the power supply is reduced. When the power is reduced to a level that can ionize ozone but not oxygen, the ozone is ionized and decomposed into oxygen, achieving the purpose of eliminating ozone.

[0069] The tissue culture room sterilization method of the present invention includes a low ozone operation mode, a high ozone operation mode and a fresh air operation mode, can be operated in corresponding modes according to the needs of the laboratory, has full automatic control, is flexible to use, and ensures that the indoor environment of the area is clean.

[0070] The above is a schematic description of the present invention and its embodiments, which is not restrictive. The drawings show only one embodiment of the present invention, and the actual structure is not limited thereto. Therefore, if a person skilled in the art is inspired by this and, without departing from the purpose of the present invention, designs a structure and embodiment similar to this technical solution without inventiveness, they shall fall within the scope of protection of the present invention.

Claims

1. A tissue culture room sterilization device, characterized in that: The invention comprises a chassis, wherein a gas collection area, a disinfection reaction area, an electrical control area and a fresh air area are provided in the chassis, the gas collection area is arranged between the fresh air area and the disinfection reaction area, a built-in fan is provided in the gas collection area, a circulating air inlet is provided on the chassis near the gas collection area, a fresh air inlet is provided in the fresh air area, a reaction module is provided in the disinfection reaction area, an air outlet is provided on the chassis near the reaction module, an ozone detector is provided at the air outlet, a narrow pulse power supply and a stand-alone controller are provided in the electrical control area, the stand-alone controller is electrically connected to the ozone detector and the narrow pulse power supply respectively, the stand-alone controller is electrically connected to the built-in fan through a frequency converter, and the output end of the narrow pulse power supply is connected to the reaction module; A module bracket is provided in the disinfection reaction area, and the reaction module includes a first fixed plate and a second fixed plate, the first fixed plate and the second fixed plate are connected to the module bracket, a wind shield is provided on the periphery of the first fixed plate or the periphery of the second fixed plate, and an array of discharge tubes is provided between the first fixed plate and the second fixed plate, a first positioning bracket is provided on one side of the first fixed plate, and a second positioning bracket is provided on one side of the second fixed plate, and a discharge electrode wire is provided between the discharge tubes, and the two ends of the discharge electrode wire are respectively connected to the first positioning bracket and the second positioning bracket, and the output end of the narrow pulse power supply is connected to the discharge electrode wire; The discharge electrode includes a pole wire body, a plurality of discharge plates are provided on the pole wire body, an intermediate clamping barrel is provided between the discharge plates, and outer clamping barrels are provided at both ends of the pole wire body. The upper and lower end faces of the intermediate clamping barrel are provided with spaced positioning grooves and positioning blocks, the positioning grooves and positioning blocks on the upper end face of the intermediate clamping barrel are staggered with the positioning grooves and positioning blocks on the lower end face of the same intermediate clamping barrel, the end face of the outer clamping barrel close to the discharge plate is provided with spaced positioning grooves and positioning blocks, the outer periphery of the discharge plate is evenly provided with discharge ends, the discharge end limit is located between the positioning grooves and positioning blocks adjacent to the discharge end, and the discharge end protrudes from the surface of the intermediate clamping barrel and the surface of the outer clamping barrel, and the discharge ends of adjacent discharge plates are staggered along the axial direction of the pole wire body.

2. A tissue culture room sterilization device according to claim 1, characterized in that: A flow balancing plate is provided between the disinfection reaction area and the gas collection area, and a plurality of flow balancing holes are provided on the flow balancing plate.

3. A tissue culture room sterilization device according to claim 1, characterized in that: The fresh air zone is provided with a fresh air outlet flange, and the fresh air outlet flange is provided with a fresh air outlet adapter.

4. A tissue culture room sterilization device according to claim 1, characterized in that: A fan bracket is provided in the gas collection area, and the built-in fan is fixed on the fan bracket.

5. A tissue culture room sterilization device according to claim 1, characterized in that: A power supply bracket is provided in the electrical control area, a control box is provided on the power supply bracket, and the narrow pulse power supply is located in the control box.

6. A tissue culture room sterilization system, characterized in that: It includes a main controller, a fresh air blower and at least one chamber, wherein the tissue culture chamber sterilization device according to any one of claims 1 to 5 is arranged in the chamber, the air inlet side of the fresh air blower is connected to the external air, the air outlet side of the fresh air blower is connected to the fresh air main duct, the fresh air main duct is connected to the fresh air inlet through a fresh air branch duct, the fresh air branch duct is provided with a solenoid valve for controlling the conduction and closing of the fresh air branch duct, the main controller is communicatively connected to the stand-alone controller, and the main controller is electrically connected to the fresh air blower and the solenoid valve respectively.

7. A tissue culture room sterilization system according to claim 6, characterized in that: It also includes a communication module and a control panel. The main controller is connected to the cloud background through the communication module, and the control panel is electrically connected to the main controller.

8. A method for sterilizing a tissue culture room, characterized in that: The tissue culture room sterilization system according to any one of claims 6 to 7 comprises: In the low ozone operation mode, the main controller controls the solenoid valve corresponding to the compartment to be closed; the stand-alone controller controls the operation of the built-in fan and the narrow pulse power supply. The narrow pulse power supply operates within the first output power range. The built-in fan draws in the air in the compartment from the circulating air inlet. The inhaled air passes through the flow equalizing plate to evenly distribute the airflow and send it into the disinfection reaction area. The air after equalization passes through the discharge electrode. The high-energy electrons generated by the discharge electrode bombard the oxygen and water vapor in the gas, which are activated, decomposed and ionized to produce free radicals, atomic oxygen and ozone with strong oxidizing ability. The free radicals, atomic oxygen and ozone with strong oxidizing ability undergo sufficient redox reactions with the microorganisms in the inhaled air to complete the air purification. The purified air is discharged back into the compartment from the air outlet; the ozone detector detects the ozone concentration in the air outlet in real time. The stand-alone controller adjusts the output power of the narrow pulse power supply within the first output power range according to the ozone concentration feedback from the ozone detector, and controls the ozone concentration at the air outlet to be within the first ozone threshold. In high ozone operation mode, the main controller controls the solenoid valve corresponding to the compartment to be closed; the stand-alone controller controls the operation of the built-in fan and the narrow pulse power supply. The narrow pulse power supply operates in the second output power range, and the built-in fan draws the air in the compartment from the circulating air inlet. The inhaled air passes through the flow equalizing plate to evenly distribute the airflow and send it into the disinfection reaction area. The air after equalization passes through the discharge electrode. The high-energy electrons generated by the discharge electrode bombard the oxygen and water vapor in the gas, and after activation, decomposition and ionization, free radicals, atomic oxygen and ozone with strong oxidizing ability are generated. The free radicals, atomic oxygen and ozone with strong oxidizing ability react with the inhaled air. The microorganisms in the air undergo a sufficient redox reaction, and the purified air is enriched with high-concentration ozone and is re-discharged from the air outlet into the compartment to disinfect the microorganisms in the compartment; the ozone detector detects the ozone concentration in the air outlet in real time, and the stand-alone controller adjusts the output power of the narrow pulse power supply within the second output power range according to the ozone concentration fed back by the ozone detector, and controls the ozone concentration at the air outlet to be within the second ozone threshold; after the disinfection is completed, the stand-alone controller controls the operation within the third output power range, and the ozone passing through the discharge electrode is electrolyzed into oxygen, so that the ozone concentration in the compartment is quickly reduced to zero; In the fresh air operation mode, the main controller controls the solenoid valve corresponding to the compartment to open, and controls the operation of the fresh air fan at the same time; the stand-alone controller controls the operation of the built-in fan and the narrow pulse power supply. The narrow pulse power supply operates within the first output power range, and the fresh air fan sends external air into the fresh air inlet through the fresh air main duct and the fresh air branch duct. The inhaled air passes through the flow equalizing plate to evenly distribute the airflow and is sent into the disinfection reaction area. The evenly distributed air passes through the discharge electrode. The high-energy electrons generated by the discharge electrode bombard the oxygen and water vapor in the gas, and after activation, decomposition and ionization, they produce free radicals, atomic oxygen and ozone with strong oxidizing ability. The free radicals, atomic oxygen and ozone with strong oxidizing ability undergo sufficient redox reactions with the microorganisms in the inhaled air to complete the air purification. The purified air is discharged from the air outlet into the compartment; the ozone detector detects the ozone concentration in the air outlet in real time. The stand-alone controller adjusts the output power of the narrow pulse power supply within the first output power range according to the ozone concentration feedback from the ozone detector, and controls the ozone concentration at the air outlet to be within the first ozone threshold.

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