Preparation method of sodium alginate bacteriostatic film loaded with garlicin pickering emulsion

Abstract

The application provides a preparation method of a sodium alginate bacteriostatic film loaded with a garlicin Pickering emulsion, which embeds garlicin in the Pickering emulsion to make a bacteriostatic packaging film, and the bacteriostatic performance, mechanical performance and moisture and oxygen blocking performance of the film are more excellent. The preparation method is as follows: firstly, octenyl succinate starch is dissolved in water to obtain an octenyl succinate starch particle dispersion solution; then, garlicin is added, and a homogenizer is used for dispersion for 1-5 min to obtain a Pickering emulsion; secondly, 1-3% of sodium alginate is taken out and dissolved in deionized water to obtain a sodium alginate solution by fully stirring; then, 0.5-2.5% of glycerol and 0.75-3% of nanocellulose are added to prepare a film forming solution; thirdly, the film forming solution is mixed with the Pickering emulsion to obtain a film solution; and finally, the film solution is vacuum degassed and then flows on a glass plate to form a film.

Description

A method for preparing a sodium alginate antibacterial film loaded with allicin-pickerlin emulsion Technical Field

[0001] This invention belongs to the field of food packaging materials, specifically relating to a method for preparing a sodium alginate antibacterial film loaded with allicin Pickering emulsion. Background Technology

[0002] Allicin, also known as garlic oil, is a substance isolated from garlic bulbs and has a strong odor. The chemical name of allicin is diallyl thiosulfinate (C6H2O). 12 S 20 Allicin possesses various health benefits, including antibacterial, antifungal, antiprotozoal, antitumor, anti-hyperlipidemia, and anti-atherosclerotic effects. It can inhibit and kill bacteria, exhibiting significant inhibitory and bactericidal effects against harmful bacteria such as Escherichia coli, Bacillus subtilis, Salmonella, Staphylococcus aureus, Shigella, Salmonella typhi, Streptococcus pneumoniae, and others, making it a natural broad-spectrum antibacterial agent.

[0003] However, allicin exhibits poor stability in heat and alkali environments, and is easily affected by the external environment in antibacterial packaging systems, leading to volatilization and degradation, thus reducing its bioavailability and making it difficult for allicin to exert its antibacterial effects. Therefore, to enhance the bioavailability of allicin, an appropriate encapsulation system can be selected to protect allicin and increase its bioavailability.

[0004] Allicin is unstable, so using Pickering emulsions to encapsulate it can improve its stability. Pickering emulsions, as novel emulsion stabilizers, can reduce or eliminate the need for surfactants, and their use can significantly improve the stability of substances. Pickering emulsions primarily stabilize emulsions by increasing steric hindrance, thereby altering interfacial properties, or by changing the rheological properties of the continuous phase. The particles in the stabilized emulsion used have a certain degree of wettability between the aqueous and oil phases, thus preventing emulsion droplet aggregation by adsorbing at the oil-water interface to form a relatively dense particulate film. The solid particles used to prepare Pickering emulsions are typically small, generally an order of magnitude smaller than the emulsion particle size. Pickering emulsions can be used to prevent the escape of contents and can also be used to prepare sensitive materials such as environmentally sensitive materials (pH, temperature, light / electricity / magnetism, etc.) and thermosensitive materials.

[0005] Sodium alginate is a high-molecular-weight polysaccharide compound extracted from seaweed. It boasts advantages such as wide availability, high safety, biocompatibility, and biodegradability. The sodium alginate molecule contains numerous hydrophilic groups (-OH, -COONa), making it water-soluble. However, sodium alginate exhibits poor mechanical properties as a membrane material; therefore, nanocellulose is introduced to improve its performance. Summary of the Invention

[0006] The purpose of this invention is to provide a method for preparing a sodium alginate biodegradable antibacterial film loaded with allicin Pickering emulsion, which can improve the stability of allicin and has good mechanical properties and moisture and oxygen barrier properties.

[0007] The objective of this invention is achieved through the following technical solution:

[0008] A method for preparing a sodium alginate antibacterial film loaded with allicin-pickerlin emulsion, characterized by the following specific steps:

[0009] (1) Preparation of Pickering emulsion: Octenyl succinic acid starch is dissolved in water and gelatinized in a water bath at 80-90℃ for 1 hour to obtain an octenyl succinic acid starch particle dispersion solution; then an oil phase is added, and the mixture is dispersed in a homogenizer for 1-5 minutes to obtain a Pickering emulsion. The amount of oil phase added is 17-50% of the mass of octenyl succinic acid starch. The speed of the homogenizer is 13000-18000 r / min, 30 seconds each time, with a 10-second interval; the oil phase is allicin, and the mass percentage concentration of the octenyl succinic acid starch particle dispersion solution is 10-30%.

[0010] (2) Preparation of Pickering emulsion membrane: Dissolve sodium alginate (1-3% by mass) in deionized water and stir thoroughly at 25°C for 4-5 hours to obtain sodium alginate solution. Add 0.5-2.5% glycerol and 0.75-3% nanocellulose by volume of the solution and stir with an electric stirrer for 15-45 minutes to prepare the film-forming solution. Mix the film-forming solution with the Pickering emulsion obtained in (1), with a volume ratio of 2.5:100-10:100. Mix with an electric stirrer for 0.5-1.5 hours and then sonicate with an ultrasonic probe to prepare the film solution for the Pickering emulsion membrane.

[0011] (3) Preparation of Pickering emulsion membrane: The membrane solution of the Pickering emulsion membrane obtained in (2) was degassed under vacuum and then cast onto a glass plate to form a film, wherein the film casting flow rate was 0.61 ml / cm. 2 The film is dried in a drying oven, cooled to room temperature, peeled off, and placed in a constant temperature and humidity chamber for equilibration for 12 hours.

[0012] As a more preferred technical solution of the present invention: the amount of oil phase added is 25% of the mass of octenyl succinic acid starch.

[0013] As a preferred technical solution of the present invention, the time for dispersing the oil phase after it is added using a homogenizer is 3 minutes.

[0014] As a more preferred technical solution of the present invention: the volume ratio of Pickering emulsion and film-forming liquid in step (3) is 5:100.

[0015] As a preferred technical solution of the present invention, the sodium alginate concentration is 1.5% by mass percentage.

[0016] As a preferred technical solution of the present invention: the amount of glycerol added is 1.5% of the solution volume; the amount of nanocellulose added is 1.5% of the solution volume.

[0017] As a preferred technical solution of the present invention, the sodium alginate solution is stirred for 5 hours.

[0018] As a preferred technical solution of the present invention: the stirring time after adding glycerol and nanocellulose is 20 minutes using an electric stirrer.

[0019] As a preferred technical solution of the present invention: the electric stirring time is 1 hour, followed by ultrasonic treatment with an ultrasonic probe.

[0020] As a more preferred technical solution of the present invention: the ultrasonic probe has an ultrasonic power of 500W and is subjected to ultrasonication for 15 minutes in a mode of 5 seconds of ultrasonication followed by 2 seconds of intermittent ultrasonication.

[0021] The beneficial effects are as follows:

[0022] The method provided by this invention improves the antibacterial stability of allicin in the packaging film through the synergistic effect of conditions such as sodium alginate concentration, nanocellulose and glycerol addition amount, and Pickering emulsion to sodium alginate ratio. Compared with directly adding allicin, encapsulating allicin in Pickering emulsion before making the packaging film results in better antibacterial performance and improved mechanical properties and moisture and oxygen barrier properties. Detailed Implementation

[0023] The following examples are used to illustrate the present invention, but are not intended to limit the scope of the invention. Unless otherwise specified, the technical means used in the examples are conventional means well known to those skilled in the art, and the raw materials used are all commercially available products.

[0024] Example 1

[0025] Step 1: Preparation of Pickering emulsion:

[0026] Dissolve 10g of octenyl succinic acid starch in 50ml of water, then gelatinize it in a water bath at 90℃ for 1h to obtain an octenyl succinic acid starch granule dispersion solution; after cooling to room temperature, add 2.5ml of allicin, and homogenize in a homogenizer for 3min at a speed of 13000r / min, for 30s each time, with a 10s interval.

[0027] Step 2: Preparation of Pickering emulsion film

[0028] (1) Weigh 3g of sodium alginate and dissolve it in 200ml of deionized water. Stir well at 25℃ for 5h to dissolve. Then add 3ml of glycerol and 3ml of nanocellulose and stir with an electric stirrer for 20min to form a film solution for later use.

[0029] (2) Take 10ml of Pickering emulsion and 200ml of membrane solution from step 2 (1) and stir thoroughly with an electric stirrer. After mixing for 1 hour, use an ultrasonic probe with a power of 500W to sonicate for 15 minutes in an ultrasonic mode of 5 seconds followed by 2 seconds of intermittent sonication.

[0030] (3) The film-forming solution of the Pickering emulsion obtained in step (2) is degassed under vacuum and then cast onto a glass plate to form a film, wherein the film-forming flow rate is 0.61 ml / cm. 2 The film was dried in a 50℃ drying oven for 8 hours, cooled to room temperature, peeled off, and placed in a 25℃ constant temperature and humidity chamber to equilibrate at 50% humidity for 12 hours.

[0031] Example 2

[0032] Step 1: Preparation of Pickering emulsion:

[0033] Dissolve 10g of octenyl succinic acid starch in 50ml of water, then gelatinize it in a water bath at 90℃ for 1h to obtain an octenyl succinic acid starch granule dispersion solution; after cooling to room temperature, add 5ml of allicin, and homogenize in a homogenizer for 3min at a speed of 13000r / min, 30s each time, with a 10s interval.

[0034] Step 2: Preparation of Pickering emulsion film

[0035] (1) Weigh 3g of sodium alginate and dissolve it in 200ml of deionized water. Stir well at 25℃ for 5h to dissolve. Then add 3ml of glycerol and 3ml of nanocellulose and stir with an electric stirrer for 20min to form a film solution for later use.

[0036] (2) Take 10ml of Pickering emulsion and 200ml of membrane solution from step 2 (1) and stir thoroughly with an electric stirrer. After mixing for 1 hour, use an ultrasonic probe with a power of 500W to sonicate for 15 minutes in an ultrasonic mode of 5 seconds followed by 2 seconds of intermittent sonication.

[0037] (3) The film-forming solution of the Pickering emulsion obtained in step (2) is degassed under vacuum and then cast onto a glass plate to form a film, wherein the film-forming flow rate is 0.61 ml / cm. 2 The film was dried in a 50℃ drying oven for 8 hours, cooled to room temperature, peeled off, and placed in a 25℃ constant temperature and humidity chamber to equilibrate at 50% humidity for 12 hours.

[0038] Example 3

[0039] Step 1: Preparation of Pickering emulsion:

[0040] Dissolve 10g of octenyl succinic acid starch in 50ml of water, then gelatinize it in a water bath at 90℃ for 1h to obtain an octenyl succinic acid starch granule dispersion solution; after cooling to room temperature, add 2.5ml of allicin, and homogenize in a homogenizer for 3min at a speed of 13000r / min, for 30s each time, with a 10s interval.

[0041] Step 2: Preparation of Pickering emulsion film

[0042] (1) Weigh 2g of sodium alginate and dissolve it in 200ml of deionized water. Stir well at 25℃ for 5h to dissolve. Then add 3ml of glycerol and 3ml of nanocellulose and stir with an electric stirrer for 20min to form a film solution for later use.

[0043] (2) Take 10ml of Pickering emulsion and 200ml of membrane solution from step 2 (1) and stir thoroughly with an electric stirrer. After mixing for 1 hour, use an ultrasonic probe with a power of 500W to sonicate for 15 minutes in an ultrasonic mode of 5 seconds followed by 2 seconds of intermittent sonication.

[0044] (3) The film-forming solution of the Pickering emulsion obtained in step (2) is degassed under vacuum and then cast onto a glass plate to form a film, wherein the film-forming flow rate is 0.61 ml / cm. 2 The film was dried in a 50℃ drying oven for 8 hours, cooled to room temperature, peeled off, and placed in a 25℃ constant temperature and humidity chamber to equilibrate at 50% humidity for 12 hours.

[0045] Example 4

[0046] Step 1: Preparation of Pickering emulsion:

[0047] Dissolve 10g of octenyl succinic acid starch in 50ml of water, then gelatinize it in a water bath at 90℃ for 1h to obtain an octenyl succinic acid starch granule dispersion solution; after cooling to room temperature, add 2.5ml of allicin, and homogenize in a homogenizer for 3min at a speed of 13000r / min, for 30s each time, with a 10s interval.

[0048] Step 2: Preparation of Pickering emulsion film

[0049] (1) Weigh 4g of sodium alginate and dissolve it in 200ml of deionized water. Stir well at 25℃ for 5h to dissolve. Then add 3ml of glycerol and 3ml of nanocellulose and stir with an electric stirrer for 20min to form a film solution for later use.

[0050] (2) Take 10ml of Pickering emulsion and 200ml of membrane solution from step 2 (1) and stir thoroughly with an electric stirrer. After mixing for 1 hour, use an ultrasonic probe with a power of 500W to sonicate for 15 minutes in an ultrasonic mode of 5 seconds followed by 2 seconds of intermittent sonication.

[0051] (3) The film-forming solution of the Pickering emulsion obtained in step (2) is degassed under vacuum and then cast onto a glass plate to form a film, wherein the film-forming flow rate is 0.61 ml / cm. 2 The film was dried in a 50℃ drying oven for 8 hours, cooled to room temperature, peeled off, and placed in a 25℃ constant temperature and humidity chamber to equilibrate at 50% humidity for 12 hours.

[0052] Example 5

[0053] Step 1: Preparation of Pickering emulsion:

[0054] Dissolve 10g of octenyl succinic acid starch in 50ml of water, then gelatinize it in a water bath at 90℃ for 1h to obtain an octenyl succinic acid starch granule dispersion solution; after cooling to room temperature, add 2.5ml of allicin, and homogenize in a homogenizer for 3min at a speed of 13000r / min, for 30s each time, with a 10s interval.

[0055] Step 2: Preparation of Pickering emulsion film

[0056] (1) Weigh 3g of sodium alginate and dissolve it in 200ml of deionized water. Stir well at 25℃ for 5h to dissolve. Then add 3ml of glycerol and 6ml of nanocellulose and stir with an electric stirrer for 20min to form a film solution for later use.

[0057] (2) Take 10ml of Pickering emulsion and 200ml of membrane solution from step 2 (1) and stir thoroughly with an electric stirrer. After mixing for 1 hour, use an ultrasonic probe with a power of 500W to sonicate for 15 minutes in an ultrasonic mode of 5 seconds followed by 2 seconds of intermittent sonication.

[0058] (3) The film-forming solution of the Pickering emulsion obtained in step (2) is degassed under vacuum and then cast onto a glass plate to form a film, wherein the film-forming flow rate is 0.61 ml / cm. 2 The film was dried in a 50℃ drying oven for 8 hours, cooled to room temperature, peeled off, and placed in a 25℃ constant temperature and humidity chamber to equilibrate at 50% humidity for 12 hours.

[0059] Example 6

[0060] Step 1: Preparation of Pickering emulsion:

[0061] Dissolve 10g of octenyl succinic acid starch in 50ml of water, then gelatinize it in a water bath at 90℃ for 1h to obtain an octenyl succinic acid starch granule dispersion solution; after cooling to room temperature, add 2.5ml of allicin, and homogenize in a homogenizer for 3min at a speed of 13000r / min, for 30s each time, with a 10s interval.

[0062] Step 2: Preparation of Pickering emulsion film

[0063] (1) Weigh 3g of sodium alginate and dissolve it in 200ml of deionized water. Stir well at 25℃ for 5h to dissolve. Then add 3ml of glycerol and 1.5ml of nanocellulose and stir with an electric stirrer for 20min to form a film solution for later use.

[0064] (2) Take 10ml of Pickering emulsion and 200ml of membrane solution from step 2 (1) and stir thoroughly with an electric stirrer. After mixing for 1 hour, use an ultrasonic probe with a power of 500W to sonicate for 15 minutes in an ultrasonic mode of 5 seconds followed by 2 seconds of intermittent sonication.

[0065] (3) The film-forming solution of the Pickering emulsion obtained in step (2) is degassed under vacuum and then cast onto a glass plate to form a film, wherein the film-forming flow rate is 0.61 ml / cm. 2 The film was dried in a 50℃ drying oven for 8 hours, cooled to room temperature, peeled off, and placed in a 25℃ constant temperature and humidity chamber to equilibrate at 50% humidity for 12 hours.

[0066] Example 7

[0067] Step 1: Preparation of Pickering emulsion:

[0068] Dissolve 10g of octenyl succinic acid starch in 50ml of water, then gelatinize it in a water bath at 90℃ for 1h to obtain an octenyl succinic acid starch granule dispersion solution; after cooling to room temperature, add 2.5ml of allicin, and homogenize in a homogenizer for 3min at a speed of 13000r / min, for 30s each time, with a 10s interval.

[0069] Step 2: Preparation of Pickering emulsion film

[0070] (1) Weigh 3g of sodium alginate and dissolve it in 200ml of deionized water. Stir well at 25℃ for 5h to dissolve. Then add 5ml of glycerol and 3ml of nanocellulose and stir with an electric stirrer for 20min to form a film solution for later use.

[0071] (2) Take 10ml of Pickering emulsion and 200ml of membrane solution from step 2 (1) and stir thoroughly with an electric stirrer. After mixing for 1 hour, use an ultrasonic probe with a power of 500W to sonicate for 15 minutes in an ultrasonic mode of 5 seconds followed by 2 seconds of intermittent sonication.

[0072] (3) The film-forming solution of the Pickering emulsion obtained in step (2) is degassed under vacuum and then cast onto a glass plate to form a film, wherein the film-forming flow rate is 0.61 ml / cm. 2 The film was dried in a 50℃ drying oven for 8 hours, cooled to room temperature, peeled off, and placed in a 25℃ constant temperature and humidity chamber to equilibrate at 50% humidity for 12 hours.

[0073] Example 8

[0074] Step 1: Preparation of Pickering emulsion:

[0075] Dissolve 10g of octenyl succinic acid starch in 50ml of water, then gelatinize it in a water bath at 90℃ for 1h to obtain an octenyl succinic acid starch granule dispersion solution; after cooling to room temperature, add 2.5ml of allicin, and homogenize in a homogenizer for 3min at a speed of 13000r / min, for 30s each time, with a 10s interval.

[0076] Step 2: Preparation of Pickering emulsion film

[0077] (1) Weigh 3g of sodium alginate and dissolve it in 200ml of deionized water. Stir well at 25℃ for 5h to dissolve. Then add 1ml of glycerol and 3ml of nanocellulose and stir with an electric stirrer for 20min to form a film solution for later use.

[0078] (2) Take 10ml of Pickering emulsion and 200ml of membrane solution from step 2 (1) and stir thoroughly with an electric stirrer. After mixing for 1 hour, use an ultrasonic probe with a power of 500W to sonicate for 15 minutes in an ultrasonic mode of 5 seconds followed by 2 seconds of intermittent sonication.

[0079] (3) The film-forming solution of the Pickering emulsion obtained in step (2) is degassed under vacuum and then cast onto a glass plate to form a film, wherein the film-forming flow rate is 0.61 ml / cm. 2 The film was dried in a 50℃ drying oven for 8 hours, cooled to room temperature, peeled off, and placed in a 25℃ constant temperature and humidity chamber to equilibrate at 50% humidity for 12 hours.

[0080] Example 9

[0081] Step 1: Preparation of Pickering emulsion:

[0082] Dissolve 10g of octenyl succinic acid starch in 50ml of water, then gelatinize it in a water bath at 90℃ for 1h to obtain an octenyl succinic acid starch granule dispersion solution; after cooling to room temperature, add 2.5ml of allicin, and homogenize in a homogenizer for 3min at a speed of 13000r / min, for 30s each time, with a 10s interval.

[0083] Step 2: Preparation of Pickering emulsion film

[0084] (1) Weigh 3g of sodium alginate and dissolve it in 200ml of deionized water. Stir well at 25℃ for 5h to dissolve. Then add 3ml of glycerol and 3ml of nanocellulose and stir with an electric stirrer for 20min to form a film solution for later use.

[0085] (2) Take 20ml of Pickering emulsion and 200ml of membrane solution from step 2 (1) and stir them thoroughly with an electric stirrer. After mixing for 1 hour, use an ultrasonic probe with a power of 500W to sonicate for 15 minutes in an ultrasonic mode of 5 seconds followed by 2 seconds of intermittent sonication.

[0086] (3) The film-forming solution of the Pickering emulsion obtained in step (2) is degassed under vacuum and then cast onto a glass plate to form a film, wherein the film-forming flow rate is 0.61 ml / cm. 2 The film was dried in a 50℃ drying oven for 8 hours, cooled to room temperature, peeled off, and placed in a 25℃ constant temperature and humidity chamber to equilibrate at 50% humidity for 12 hours.

[0087] Example 10: Control Group

[0088] (1) Weigh 3g of sodium alginate and dissolve it in 200ml of deionized water. Stir well at 25℃ for 5h to dissolve. Then add 3ml of glycerol and 3ml of nanocellulose and stir with an electric stirrer for 20min to form a film solution for later use. Add 1ml of allicin and continue stirring for 1h to form a film solution for later use.

[0089] (2) After vacuum degassing the film-forming solution, it is deposited onto a glass plate to form a film, wherein the film-forming flow rate is 0.61 ml / cm. 2 The film was dried in a 50℃ drying oven for 8 hours, cooled to room temperature, peeled off, and placed in a 25℃ constant temperature and humidity chamber to equilibrate at 50% humidity for 12 hours.

[0090] The mechanical properties and moisture and oxygen barrier properties of the smart packaging films prepared in Examples 1 to 10 are shown in Table 1.

[0091] The membrane thickness was determined according to the national standard "Determination of Thickness of Plastic Films and Sheets - Mechanical Measurement Method" (GB / T6672-2001). The tensile strength and elongation at break of the membrane were determined using a tensile testing machine according to the national standard "Test Method for Tensile Properties of Plastic Films" (GB / 13022-1991). The water vapor transmission rate of the membrane was determined using a water vapor transmission meter according to the national standards "Test Method for Water Vapor Permeability of Plastic Films and Sheets - Cup Method" (GB1037-1988) and "Test Method for Packaging Materials - Moisture Permeability" (GB / T16928-1997). The oxygen permeability of the membrane was determined using a GDP-C type gas permeability meter according to the national standards "Test Method for Oxygen Permeability of Packaging Materials, Plastic Films and Sheets" (GB / T19789-2005) and "Test Method for Gas Permeability of Plastic Films and Sheets - Differential Pressure Method" (GB / T1038-2000).

[0092] Table 1

[0093]

[0094] As shown in Table 1, the products obtained in Examples 1 to 9 have good mechanical properties and moisture and oxygen barrier properties, which are superior to those of the control group Example 10.

[0095] The results of the antimicrobial zone test of the packaging films prepared in Examples 1 to 10 are shown in Table 2.

[0096] Table 2

[0097]

[0098] As shown in Table 2, compared with the control group Example 10, the products obtained in Examples 1 to 9 have larger inhibition zones and better antibacterial effects.

[0099] Finally, the method of this invention is merely a preferred embodiment and is not intended to limit the scope of protection of this invention. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of this invention should be included within the scope of protection of this invention.

Claims

1. A method for preparing a sodium alginate antibacterial film loaded with allicin-pickerlin emulsion, characterized in that, The specific steps are as follows: (1) Preparation of Pickering emulsion: After dissolving octenyl succinic acid starch in water, gelatinize it in a water bath at 80-90℃ for 1 hour to obtain an octenyl succinic acid starch particle dispersion solution; then add an oil phase, and then disperse it in a homogenizer for 1-5 minutes to obtain a Pickering emulsion. The amount of oil phase added is 17-50% of the mass of octenyl succinic acid starch. The speed of the homogenizer is 13000-18000 r / min, 30s each time, with an interval of 10s; the oil phase is allicin, and the mass percentage concentration of the octenyl succinic acid starch particle dispersion solution is 10-30%; (2) Preparation of Pickering emulsion film: Add 1.5% by mass of allicin to the oil phase. Sodium alginate was dissolved in deionized water and stirred thoroughly at 25°C for 4-5 hours to obtain a sodium alginate solution. 1.5-2.5% glycerol and 0.75-3% nanocellulose were added by volume and stirred with an electric stirrer for 15-45 minutes to prepare a film-forming solution. The film-forming solution was mixed with the Pickering emulsion obtained in (1), with a volume ratio of 10:

100. After mixing with an electric stirrer for 0.5-1.5 hours, the mixture was ultrasonically treated with an ultrasonic probe to prepare the film solution for the Pickering emulsion membrane. (3) Preparation of the Pickering emulsion membrane: The film solution of the Pickering emulsion membrane obtained in (2) was degassed under vacuum and then cast onto a glass plate to form a film, with a film casting flow rate of 0.61 ml / cm. 2 The film is dried in a drying oven, cooled to room temperature, peeled off, and placed in a constant temperature and humidity chamber for equilibration for 12 hours.

2. The method for preparing a sodium alginate antibacterial film loaded with allicin Pickering emulsion as described in claim 1, characterized in that: The amount of oil phase added is 25% of the mass of octenyl succinic acid starch.

3. The method for preparing a sodium alginate antibacterial film loaded with allicin Pickering emulsion as described in claim 1, characterized in that: The time for homogenizing the oil phase after its addition is 3 minutes.

4. The method for preparing a sodium alginate antibacterial film loaded with allicin Pickering emulsion as described in claim 1, characterized in that: The amount of glycerol added is 1.5% of the solution volume; the amount of nanocellulose added is 1.5% of the solution volume.

5. The method for preparing a sodium alginate antibacterial film loaded with allicin Pickering emulsion as described in claim 1, characterized in that: The sodium alginate solution was stirred for 5 hours.

6. The method for preparing a sodium alginate antibacterial film loaded with allicin Pickering emulsion as described in claim 1, characterized in that: The process of adding glycerol and nanocellulose and then stirring with an electric stirrer for 20 minutes is described above.

7. The method for preparing a sodium alginate antibacterial film loaded with allicin Pickering emulsion as described in claim 1, characterized in that: The electric stirring time is 1 hour, followed by ultrasonic treatment with an ultrasonic probe.

8. The method for preparing a sodium alginate antibacterial film loaded with allicin Pickering emulsion as described in claim 1, characterized in that: The ultrasonic probe has an ultrasonic power of 500W and is used in an ultrasonic mode of 5 seconds of ultrasound followed by 2 seconds of intermittent ultrasound for 15 minutes.

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