A detoxification agent for aflatoxin M1 in liquid milk and a preparation method and application thereof

Abstract

The application discloses a detoxification agent for aflatoxin M1 in liquid milk and a preparation method and application thereof. The detoxification agent for aflatoxin M1 in liquid milk comprises agar, sodium bicarbonate and montmorillonite, and the mass fractions of the agar, the sodium bicarbonate and the montmorillonite are 0.8-0.9 parts, 6-7 parts and 10-18 parts respectively. The detoxification agent provided by the application comprises agar, sodium bicarbonate and montmorillonite, wherein the montmorillonite mainly serves as a detoxification material and plays a role in removing aflatoxin M1 in liquid milk; the agar and the sodium bicarbonate mainly serve as shaping materials of the detoxification agent and serve as a supporting framework of the detoxification material, have a porous structure, and have a large specific gravity ratio and a large surface area; when the obtained detoxification agent is applied to removal of aflatoxin M1 in liquid milk, the detoxification rate is high, the detoxification agent can be recycled, the detoxification agent is safe to use and has no residue, and has no influence on the quality of the liquid milk.

Description

Technical Field

[0001] This invention relates to the field of food processing technology, specifically to a detoxifying agent for aflatoxin M1 in liquid milk, its preparation method, and its application. Background Technology

[0002] Aflatoxin M1 (AFM1) is a potent carcinogen and has been classified as a Group 1 carcinogen by the International Agency for Research on Cancer (IARC). When animals consume feed contaminated with aflatoxin B1, it is absorbed, metabolized, and converted into AFM1, which is then transferred to meat, eggs, and milk. The presence of AFM1 in milk is of particular concern. Currently, many countries and organizations have set maximum residue limits for AFM1 in milk. China and the United States have a limit of 500 ng / L, which is 10 times the EU limit (50 ng / L). Currently, there is no data confirming how much AFM1 intake is safe for humans, but lower intake levels are generally considered safer. Summary of the Invention

[0003] The main objective of this invention is to provide a detoxifying agent for aflatoxin M1 in liquid milk, its preparation method, and its application, aiming to provide a detoxifying agent that can effectively remove aflatoxin M1 from liquid milk.

[0004] To achieve the above objectives, the present invention proposes a detoxifying agent for aflatoxin M1 in liquid milk. The detoxifying agent for aflatoxin M1 in liquid milk comprises agar, sodium bicarbonate and montmorillonite, wherein the mass parts of agar, sodium bicarbonate and montmorillonite are 0.8-0.9 parts, 6-7 parts and 10-18 parts, respectively.

[0005] Optionally, the montmorillonite is present in 10 to 11 parts by mass.

[0006] Optionally, the montmorillonite is present in 12 to 13 parts by mass.

[0007] Optionally, the montmorillonite is present in 14 to 15 parts by mass.

[0008] Optionally, the montmorillonite is present in 16 to 18 parts by mass.

[0009] Optionally, the detoxifying agent for aflatoxin M1 in liquid milk is in granular or columnar form.

[0010] To achieve the above objectives, the present invention also proposes a method for preparing a detoxifying agent for aflatoxin M1 in liquid milk as described above, comprising the following steps:

[0011] Mix agar, montmorillonite and water, heat to fully dissolve the agar, then add sodium bicarbonate and mix well to obtain a mixed solution;

[0012] The mixture is injected into a mold, allowed to stand until solidified, and then dried to obtain a pre-product.

[0013] The pre-processed product is immersed in 3-5% edible acetic acid, then drained, rinsed with water to remove residual acetic acid, and then dried to obtain a detoxifying agent for aflatoxin M1 in liquid milk.

[0014] Optionally, in the step of mixing agar, montmorillonite, and water, heating to fully dissolve the agar, then adding sodium bicarbonate and mixing thoroughly to obtain the mixed solution:

[0015] The heating temperature for fully dissolving the agar is 45~70℃.

[0016] Furthermore, the present invention also proposes a method for removing aflatoxin M1 from liquid milk, comprising the following steps:

[0017] Add the detoxifying agent to the liquid milk to be treated and stir at 500-600 rpm for 40-120 minutes. Then remove the detoxifying agent, that is, remove aflatoxin M1 from the liquid milk. The detoxifying agent is the detoxifying agent for aflatoxin M1 in liquid milk as described above.

[0018] Optionally, 0.9 to 1.5 g of the detoxifying agent may be added to every 100 mL of the liquid milk.

[0019] The technical solution provided by this invention includes agar, sodium bicarbonate, and montmorillonite as the detoxifying agent for aflatoxin M1 in liquid milk. Montmorillonite is mainly used as the detoxifying material to remove aflatoxin M1 from liquid milk. Agar and sodium bicarbonate are mainly used as shaping materials for the detoxifying agent, serving as the supporting framework for the detoxifying agent. They have a porous structure, a high density, and a large surface area. When the detoxifying agent obtained is applied to remove aflatoxin M1 from liquid milk, it has a high detoxification rate, is recyclable, safe to use, leaves no residue, and has no impact on the quality of the liquid milk itself. Attached Figure Description

[0020] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For those skilled in the art, other related drawings can be obtained based on these drawings without creative effort.

[0021] Figure 1 This is a schematic flowchart of an embodiment of the method for preparing a detoxifying agent for aflatoxin M1 in liquid milk provided by the present invention.

[0022] The realization of the objective, functional features and advantages of the present invention will be further explained in conjunction with the embodiments and with reference to the accompanying drawings. Detailed Implementation

[0023] To make the objectives, technical solutions, and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below. Where specific conditions are not specified in the embodiments, conventional conditions or conditions recommended by the manufacturer shall apply. Where the manufacturers of reagents or instruments are not specified, they are all conventional products that can be purchased commercially. Furthermore, the meaning of "and / or" throughout the text includes three parallel solutions; for example, "A and / or B" includes solution A, or solution B, or a solution where both A and B are satisfied simultaneously. In addition, the technical solutions of the various embodiments can be combined with each other, but this must be based on the ability of those skilled in the art to implement them. When the combination of technical solutions is contradictory or cannot be implemented, it should be considered that such a combination of technical solutions does not exist and is not within the scope of protection claimed by the present invention. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0024] Aflatoxin M1 (AFM1) is a potent carcinogen and has been classified as a Group 1 carcinogen by the International Agency for Research on Cancer (IARC). When animals consume feed contaminated with aflatoxin B1, it is absorbed, metabolized, and converted into AFM1, which is then transferred to meat, eggs, and milk. The presence of AFM1 in milk is of particular concern. Currently, many countries and organizations have set maximum residue limits for AFM1 in milk. China and the United States have a limit of 500 ng / L, which is 10 times the EU limit (50 ng / L). Currently, there is no data confirming how much AFM1 intake is safe for humans, but lower intake levels are generally considered safer.

[0025] Therefore, the present invention aims to develop a safe, efficient, and residue-free detoxification material for AFM1 in liquid milk. Specifically, the present invention proposes a detoxifying agent for aflatoxin M1 in liquid milk. The detoxifying agent for aflatoxin M1 in liquid milk comprises agar, sodium bicarbonate, and montmorillonite, wherein the mass fractions of agar, sodium bicarbonate, and montmorillonite are 0.8-0.9 parts, 6-7 parts, and 10-18 parts, respectively.

[0026] In the technical solution provided by this invention, the detoxifying agent for AFM1 in liquid milk includes agar, sodium bicarbonate, and montmorillonite. Agar and sodium bicarbonate primarily serve as shaping materials for the detoxifying agent, acting as a supporting framework. They possess a porous structure, a high bulk density (up to 37.6 ± 1.4), and a large surface area. Montmorillonite, on the other hand, primarily acts as the detoxifying material, adhering to the agar surface to remove AFM1 from the liquid milk. When liquid milk containing AFM1 passes through the micropores of the detoxifying agent, AFM1 is adsorbed by the montmorillonite, thereby reducing the AFM1 content in the liquid milk. Therefore, the detoxifying agent provided by this invention achieves a high detoxification rate when applied to remove AFM1 from liquid milk. Furthermore, the detoxifying agent is recyclable, safe to use, leaves no residue, and has no impact on the quality of the liquid milk itself. It is particularly suitable for removing AFM1 from raw milk, reducing the AFM1 content in subsequent dairy products such as pasteurized milk, UHT milk, yogurt, and cheese.

[0027] It should be noted that the bulk density ratio mentioned in this article is defined as: bulk density ratio = (weight of plasticizer after absorbing water (g) - weight of plasticizer) / weight of plasticizer (g); the larger the bulk density ratio, the more pores and the larger the surface area.

[0028] Further, in the first embodiment of the present invention, the montmorillonite is 10-11 parts by mass. In the second embodiment of the present invention, the montmorillonite is 12-13 parts by mass. In the third embodiment of the present invention, the montmorillonite is 14-15 parts by mass. In the fourth embodiment of the present invention, the montmorillonite is 16-18 parts by mass. The detoxifying agents in the above four embodiments can effectively remove AFM1 from liquid milk. Further experimental verification shows that the detoxifying efficiencies of the detoxifying agents in the above four embodiments for removing AFM1 from milk are 45.6%, 56.6%, 65.8%, and 71%, respectively, with the detoxifying agent in the fourth embodiment showing the highest detoxification efficiency for AFM1 in milk. Furthermore, the effects of the detoxifying agents in the four embodiments described above on the conventional milk components in milk are shown in Table 1 below (the amount of detoxifying agent added is 1g per 100mL of milk, the stirring speed is 550rpm, and the stirring time is 80min). It can be seen that the detoxifying agents in the four embodiments do not affect the conventional milk components in milk. In addition, this invention also attempted to further increase the amount of montmorillonite added based on the four embodiments described above, and found that the particles were difficult to form and easily broken.

[0029] Table 1. Effects of four implementation methods on conventional milk components in milk.

[0030] ;

[0031] In addition, in the technical solution of the present invention, the detoxifying agent for aflatoxin M1 in liquid milk can have a variety of forms, including but not limited to granular or columnar. When the detoxifying agent for aflatoxin M1 in liquid milk is columnar, the cross-section of the column can be circular (i.e., the detoxifying agent is cylindrical), square, triangular, polygonal, etc. Furthermore, when the detoxifying agent for aflatoxin M1 in liquid milk is used, stored, transported, or sold as a product, it can be presented in bagged form. This involves placing a certain amount of the detoxifying agent into a mesh bag, or further packaging it to obtain the detoxifying agent product. The bag volume is not specifically limited; for example, each bag can contain 1g, 1.5g, 2g, 2.5g, 3g, 3.5g, 4g, 4.5g, 5g, 5.5g, or 6g, etc. When actually used for AFM1 detoxification in liquid milk, the corresponding dosage of detoxifying agent should be used according to actual needs. It is generally recommended to add 0.9~1.5g of the detoxifying agent per 100mL of liquid milk. Additionally, the mesh size of the mesh bag is not specifically limited and can be selected according to the size of the detoxifying agent. In some embodiments of the present invention, for example, a 40-mesh mesh bag can be selected. In addition, a small bag containing color-changing silica gel can be placed inside the detoxifier pouch to keep the detoxifier dry. When using, simply remove the color-changing silica gel pouch.

[0032] Based on the AFM1 detoxifying agent for liquid milk provided above by the present invention, the present invention also proposes a method for preparing the AFM1 detoxifying agent for liquid milk. Figure 1 This illustration shows an embodiment of the method for preparing an AFM1 detoxifying agent for liquid milk provided by the present invention. See also... Figure 1 As shown, in this embodiment, the preparation method of the detoxifying agent for AFM1 in liquid milk includes the following steps:

[0033] Step S10: Mix agar, montmorillonite and water, heat to fully dissolve the agar, then add sodium bicarbonate and mix well to obtain a mixed solution;

[0034] Step S20: Inject the mixture into a mold, let it stand until solidified, and then dry it to obtain a pre-product;

[0035] Step S30: Immerse the pre-processed product in 3-5% edible acetic acid, drain it, rinse it with water to remove residual acetic acid, and then dry it to obtain a detoxifying agent for aflatoxin M1 in liquid milk.

[0036] First, agar, montmorillonite, and water are mixed in a specific ratio, with the mass fractions of agar, montmorillonite, and water being 0.8-0.9 parts, 10-18 parts, and 60 parts, respectively. The mixture is then heated to fully dissolve the agar, followed by the addition of 6-7 parts of sodium bicarbonate. The mixture is then thoroughly mixed to obtain a liquid mixture. Next, the liquid mixture is injected into a mold. The mold can be selected according to the desired form of the detoxifying agent; for example, if the detoxifying agent needs to be made into granules or columns, the liquid mixture can be injected into a slotted mold with a width of 3 mm and a height of 3 mm. After injection, the mixture is allowed to solidify, then removed from the mold to form small granules, which are then dried to obtain a pre-product. Next, the pre-product is immersed in a 3-5% edible acetic acid solution, drained, and rinsed with water to remove residual acetic acid. After rinsing, it is dried again to obtain the detoxifying agent granules. The detoxifying agent preparation method provided by this invention is simple, environmentally friendly, and easily scalable for industrial production. It is understood that the drying method in the above steps can be any common method in the field, including but not limited to heating drying, microwave drying, vacuum drying, etc., and can be selected according to actual production needs.

[0037] Furthermore, in some embodiments of the present invention, after experimental verification, the preferred heating temperature for fully dissolving the agar is 45~70℃. Within this temperature range, it is more beneficial to ensure the plasticity and density ratio of the agar and sodium bicarbonate as shaping materials, thereby ensuring the detoxification effect of the final detoxifying agent. Additionally, when injecting the mixture into the mold in step S20, it is preferable to control the material temperature to be maintained at 45~70℃ during this process to ensure the material's fluidity. It should be noted that the temperature in this process can be the same as or different from the heating temperature in step S10, as long as both are within the range of 45~70℃.

[0038] Furthermore, based on the detoxifying agent for AFM1 in liquid milk provided above, this invention also proposes a method for removing aflatoxin M1 from liquid milk, using the aforementioned detoxifying agent for AFM1 in liquid milk, specifically including the following steps:

[0039] Add the detoxifying agent to the liquid milk to be treated and stir at 500-600 rpm for 40-120 minutes. Then remove the detoxifying agent, that is, remove aflatoxin M1 from the liquid milk.

[0040] When the detoxifying agent is presented in a mesh bag, it can be directly added to the liquid milk to be treated and stirred to achieve detoxification. Preferably, the detoxifying agent can be rinsed before being added to the liquid milk to remove dust from its surface and avoid contaminating the liquid milk product. Specifically, the mesh bag containing the detoxifying agent can be rinsed with purified water until the filtered water is clear before proceeding with the subsequent detoxification treatment. When the detoxifying agent is presented in the form of granules or columnar particles, it can be dispensed into mesh bags, with a certain dosage of the detoxifying agent placed in the mesh bag before rinsing and subsequent detoxification treatment.

[0041] The detoxifying agent provided by this invention is used to remove AFM1 from liquid milk. The method is simple, easy to recover, and has a high detoxification rate, is safe, and leaves no residue. The method for removing aflatoxin M1 from liquid milk provided by this invention is applicable to various dairy products, including but not limited to cow's milk and goat's milk. In some embodiments of this invention, it is preferred to remove AFM1 from cow's milk, which has the advantages of easy recovery, high detoxification rate, safety and no residue, and does not affect the conventional milk components in cow's milk.

[0042] In some embodiments of the present invention, through comparative experiments, it was found that the amount of the detoxifying agent added is preferably controlled as follows: 0.9~1.5g of the detoxifying agent is added to every 100mL of the liquid milk. Within this range, the detoxifying agent can effectively remove AFM1 from the liquid milk, with a detoxification rate of up to 86.6%, and will not significantly affect the quality of the liquid milk itself.

[0043] Furthermore, in the technical solution of this invention, the addition of the detoxifying agent can be completed in one go or in stages, depending on the actual detoxification requirements. Specifically, a one-time addition means adding the corresponding dose of detoxifying agent to the liquid milk to be treated at once, and stirring at 500-600 rpm for 40-120 minutes to complete the AFM1 detoxification treatment in the liquid milk. The number of stages is not limited; for example, it can be two or three times. Taking two-stage detoxification as an example, the specific steps are as follows: after taking the detoxifying agent according to the dosage, first add a certain dose of the detoxifying agent to the liquid milk to be treated, and stir at 500-600 rpm for 40-120 minutes. Then, add the remaining dose of detoxifying agent to the liquid milk, and continue stirring at 500-600 rpm for another 40-120 minutes to complete the AFM1 detoxification treatment in the liquid milk. When using two or more stages of detoxification, the stirring time after each addition of the detoxifying agent is 40-120 minutes. In some embodiments of the present invention, when a two-stage or more-stage detoxification method is used, it is preferable that the dosage of the detoxifying agent added each time is the same.

[0044] The technical solution of the present invention will be further described in detail below with reference to specific embodiments and accompanying drawings. It should be understood that the following embodiments are only used to explain the present invention and are not intended to limit the present invention.

[0045] Example 1

[0046] (1) Mix 0.8 parts of agar, 10 parts of montmorillonite and 60 parts of water, then heat to 45°C and stir to fully dissolve the agar. Add 6 parts of sodium bicarbonate and mix well to obtain a mixed liquid.

[0047] (2) The prepared mixture is injected into a slot mold with a width of 3 mm and a height of 3 mm at a temperature of 50°C, left to stand until the material solidifies, then taken out of the mold, cut into small particles, then frozen and dried to obtain the pre-made product.

[0048] (3) Immerse the prepared product in 3% edible acetic acid, drain and rinse with water to remove residual acetic acid. After rinsing, freeze and dry to obtain detoxifying agent granules.

[0049] Example 2

[0050] (1) Mix 0.9 parts of agar, 11 parts of montmorillonite and 60 parts of water, then heat to 55°C and stir to fully dissolve the agar. Then add 7 parts of sodium bicarbonate and mix evenly to obtain a mixed liquid.

[0051] (2) The prepared mixture is injected into a slot mold with a width of 3 mm and a height of 3 mm at a temperature of 55°C, left to stand until the material solidifies, then taken out from the mold, cut into small particles, then frozen and dried to obtain the pre-made product.

[0052] (3) Immerse the prepared product in 4% edible acetic acid, drain and rinse with water to remove residual acetic acid. After rinsing, freeze and dry to obtain detoxifying agent granules.

[0053] Example 3

[0054] (1) Mix 0.83 parts of agar, 13 parts of montmorillonite and 60 parts of water, then heat to 60°C and stir to fully dissolve the agar. Then add 6.5 parts of sodium bicarbonate and mix evenly to obtain a mixed liquid.

[0055] (2) The prepared mixture is injected into a slot mold with a width of 3 mm and a height of 3 mm at a temperature of 60°C. The mixture is left to stand until it solidifies, then removed from the mold, cut into small particles, and then frozen and dried to obtain a pre-processed product.

[0056] (3) Immerse the prepared product in 5% edible acetic acid, drain and rinse with water to remove residual acetic acid. After rinsing, freeze and dry to obtain detoxifying agent granules.

[0057] Example 4

[0058] (1) Mix 0.8 parts of agar, 15 parts of montmorillonite and 60 parts of water, then heat to 65°C and stir to fully dissolve the agar. Add 7 parts of sodium bicarbonate and mix evenly to obtain a mixed liquid.

[0059] (2) The prepared mixture is injected into a slot mold with a width of 3 mm and a height of 3 mm at a temperature of 65°C, left to stand until the material solidifies, then taken out from the mold, cut into small particles, then frozen and dried to obtain the pre-made product.

[0060] (3) Immerse the prepared product in 4% edible acetic acid, drain and rinse with water to remove residual acetic acid. After rinsing, freeze and dry to obtain detoxifying agent granules.

[0061] Example 5

[0062] (1) Mix 0.9 parts of agar, 18 parts of montmorillonite and 60 parts of water, then heat to 70°C and stir to fully dissolve the agar. Then add 6.8 parts of sodium bicarbonate and mix evenly to obtain a mixed liquid.

[0063] (2) The prepared mixture is injected into a slot mold with a width of 3 mm and a height of 3 mm at a temperature of 70°C. The mixture is left to stand until it solidifies, then removed from the mold, cut into small particles, and then frozen and dried to obtain a pre-processed product.

[0064] (3) Immerse the prepared product in 4% edible acetic acid, drain and rinse with water to remove residual acetic acid. After rinsing, freeze and dry to obtain detoxifying agent granules.

[0065] Example 6

[0066] (1) Add AFM1 standard to the blank milk to obtain a milk sample contaminated with AFM1 at 590.6 ng / L, which is used as the milk to be treated.

[0067] (2) Take 0.9g of the detoxifying agent prepared in Example 2 and divide it equally into four 40-mesh gauze bags. Rinse the gauze bags containing the detoxifying agent with pure water until the filtered water is clear. Then, put all the gauze bags containing the detoxifying agent into 100mL of milk to be treated at once and stir at 550rpm for 80min. After stirring, take out the gauze bags containing the detoxifying agent to complete the detoxification treatment of AFM1 in the milk.

[0068] Example 7

[0069] The steps are basically the same as in Example 6, except that 1.2g of the detoxifying agent is added to 100mL of the milk to be treated.

[0070] Example 8

[0071] The steps are basically the same as in Example 6, except that 1.5g of the detoxifying agent is added to 100mL of the milk to be treated.

[0072] Example 9

[0073] The steps are basically the same as in Example 6, except that the stirring time is 50 minutes.

[0074] Example 10

[0075] The steps are basically the same as in Example 6, except that the stirring time is 110 min.

[0076] Example 11

[0077] The steps are basically the same as in Example 6, except that 1.2g of the detoxifying agent is added to 100mL of milk to be treated, and the stirring time is 50min.

[0078] Example 12

[0079] The steps are basically the same as in Example 6, except that 1.2g of the detoxifying agent is added to 100mL of the milk to be treated, and the stirring time is 110min.

[0080] Example 13

[0081] The steps are basically the same as in Example 6, except that 1.5g of the detoxifying agent is added to 100mL of the milk to be treated, and the stirring time is 50min.

[0082] Example 14

[0083] The steps are basically the same as in Example 6, except that 1.5g of the detoxifying agent is added to 100mL of the milk to be treated, and the stirring time is 110min.

[0084] Example 15

[0085] (1) Add AFM1 standard to the blank milk to obtain a milk sample contaminated with AFM1 at 590.6 ng / L, which is used as the milk to be treated.

[0086] (2) Take 0.9g of the detoxifying agent prepared in Example 2 and divide it equally into four 40-mesh gauze bags. Rinse the gauze bags containing the detoxifying agent with pure water until the filtered water is clear. Then, put two gauze bags containing the detoxifying agent into 100mL of milk to be treated and stir at 550rpm for 80min. After stirring, put the remaining two gauze bags containing the detoxifying agent into the milk and continue stirring at 550rpm for 80min. After stirring, take out the gauze bags containing the detoxifying agent to complete the detoxification treatment of AFM1 in the milk.

[0087] Example 16

[0088] The steps are basically the same as in Example 15, except that 1.2g of the detoxifying agent is added to 100mL of milk to be treated.

[0089] Example 17

[0090] The steps are basically the same as in Example 15, except that 1.5g of the detoxifying agent is added to 100mL of the milk to be treated.

[0091] Example 18

[0092] The steps are basically the same as in Example 15, except that the stirring time is 50 minutes.

[0093] Example 19

[0094] The steps are basically the same as in Example 15, except that the stirring time is 110 min.

[0095] Example 20

[0096] The steps are basically the same as in Example 15, except that 1.2g of the detoxifying agent is added to 100mL of milk to be treated, and the stirring time is 50min.

[0097] Example 21

[0098] The steps are basically the same as in Example 15, except that 1.2g of the detoxifying agent is added to 100mL of the milk to be treated, and the stirring time is 110min.

[0099] Example 22

[0100] The steps are basically the same as in Example 15, except that 1.5g of the detoxifying agent is added to 100mL of the milk to be treated, and the stirring time is 50min.

[0101] Example 22

[0102] The steps are basically the same as in Example 15, except that 1.5g of the detoxifying agent is added to 100mL of the milk to be treated, and the stirring time is 50min.

[0103] Milk samples containing the detoxifying agent were taken after stirring in Examples 6 to 23. After centrifugation to remove milk fat, the concentration of AFM1 was measured. The concentration of the AFM1 positive control group (i.e., AFM1 contaminated milk sample) was used as the initial concentration, and the detoxification efficiency was calculated. The results are shown in Table 2 below.

[0104] Table 2 Detoxification efficiency of each embodiment

[0105] ;

[0106] Milk samples containing the detoxifying agent were taken after stirring in Examples 6 to 23 and analyzed for milk composition. The results are shown in Table 3 below.

[0107] Table 3. Effects of each example on milk composition

[0108] ;

[0109] As can be seen from Tables 2 and 3, the detoxifying agent prepared in the embodiments of the present invention has a good detoxification effect when used for AFM1 detoxification in milk, with a detoxification rate of up to 86.6%, and does not affect the conventional milk components of milk.

[0110] The above are merely preferred embodiments of the present invention and do not limit the patent scope of the present invention. Various modifications and variations can be made to the present invention by those skilled in the art. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the patent protection scope of the present invention.

Claims

1. A detoxifying agent for aflatoxin M1 in liquid milk, characterized in that, The detoxifying agent for aflatoxin M1 in liquid milk includes agar, sodium bicarbonate and montmorillonite, wherein the mass parts of agar, sodium bicarbonate and montmorillonite are 0.8-0.9 parts, 6-7 parts and 10-18 parts, respectively. The method for preparing the detoxifying agent for aflatoxin M1 in liquid milk includes the following steps: Mix agar, montmorillonite and water, heat to fully dissolve the agar, then add sodium bicarbonate and mix well to obtain a mixed solution; The mixture is injected into a mold, allowed to stand until solidified, and then dried to obtain a pre-product. The pre-processed product is immersed in 3-5% edible acetic acid, then drained, rinsed with water to remove residual acetic acid, and then dried to obtain a detoxifying agent for aflatoxin M1 in liquid milk. In the step of mixing agar, montmorillonite and water, heating to fully dissolve the agar, then adding sodium bicarbonate and mixing evenly to obtain a mixed liquid: the heating temperature for fully dissolving the agar is 45-70℃. The detoxifying agent for aflatoxin M1 in liquid milk is in granular or columnar form.

2. A method for removing aflatoxin M1 from liquid milk, characterized in that, Includes the following steps: Add the detoxifying agent to the liquid milk to be treated and stir at 500-600 rpm for 40-120 minutes. Then remove the detoxifying agent, that is, remove aflatoxin M1 from the liquid milk. The detoxifying agent is the detoxifying agent for aflatoxin M1 in liquid milk as described in claim 1.

3. The method for removing aflatoxin M1 from liquid milk as described in claim 2, characterized in that, 0.9 to 1.5 g of the detoxifying agent is added to every 100 mL of the liquid milk; and / or, the liquid milk is cow's milk.

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