A composition with pore-refining effect, nano-microemulsion, and preparation method and application thereof

Abstract

The present invention relates to a composition with the effect of refining pores, a nano-microemulsion, and a preparation method and application thereof. The composition with the effect of refining pores comprises a serrata fucus extract, dipalmitoyl hydroxyproline and dipeptide diaminobutyryl benzylamide diacetate. The serrata fucus extract balances skin oil and plays a role in shrinking pores from the root; dipalmitoyl hydroxyproline is an important raw material for synthesizing collagen, which supplements collagen to support pores; dipeptide diaminobutyryl benzylamide diacetate can smooth the skin. The three have a synergistic promotion effect on the effect of refining pores that is difficult for technicians in this field to predict, and more effectively smooth the skin and shrink pores. The present invention also uses microemulsion technology to encapsulate the above composition to obtain a nano-microemulsion product, which can improve the stability of the composition, help the composition stay and penetrate at the pores, improve the permeability, enrich the sebaceous glands, and slowly release it for a long time.

Description

Technical Field

[0001] The present invention belongs to the technical field of cosmetics, and relates to a composition with pore-refining effect, a nano-microemulsion, a preparation method and application thereof, and specifically relates to a composition with pore-refining effect, a nano-microemulsion with pore-refining effect and a preparation method thereof, and an essence milk with pore-refining effect and a preparation method thereof. Background Art

[0002] Enlarged facial pores are a common skin problem and an important manifestation of skin aging. The cause may be related to excessive skin oil secretion, skin inflammation and skin aging. With age, the elasticity around the pores decreases, the volume of hair follicles increases, collagen is lost, the support around the pores is lost, and the integrity of the skin structure is destroyed. Therefore, skin aging often leads to enlarged pores.

[0003] Existing products for fine pores are mostly astringent, exfoliating and keratinizing cosmetics, which basically solve the pore problem from the surface. Among them, astringent cosmetics mostly use organic acids to coagulate proteins, which temporarily shrink the pores, but cannot solve the problem of large pores from the root; exfoliating cosmetics mostly use like to dissolve the keratin plugs blocked in the pores, and remove them by dissolving the keratin plugs with oil, but if the oil secretion cannot be regulated, it will soon regenerate. If not handled properly, the pores will become larger; and exfoliating cosmetics mostly contain salicylic acid, fruit acid, etc., which dissolve and soften the dead skin accumulated on the surface of the skin and unclog the clogged pores, but they are very irritating to the skin and can easily cause damage to sensitive skin. In addition, many existing products for fine pores have relatively single ingredients and do not have synergistic effects.

[0004] For example, CN103932954A discloses a cosmetic that can whiten and rejuvenate the skin and refine pores, and a method for making the same, including: adding VC ethyl ether, tranexamic acid, arbutin and mulberry extract to water, heating to 70°C to dissolve, and forming an aqueous phase liquid; mixing licorice flavonoids, phenylethyl resorcinol, propylene glycol and glycerol and heating to 85°C to dissolve, and forming an oil phase liquid; mixing and stirring the aqueous phase liquid and the oil phase liquid evenly, putting them into a fermentation tank, adding kojic acid and glucose, and fermenting at 30°C for 3 days to form a base liquid; drying and grinding bearberry leaves, licorice, ginseng and snow lotus into powder, adding endothelin antagonists, and fully mixing to form powder; evenly sprinkling the powder into the base liquid to obtain the cosmetic. However, the endothelin antagonists used in the invention are prone to adverse reactions such as edema, and the safety aspect needs to be verified.

[0005] Natural ingredients with the effect of refining pores can better overcome the above disadvantages, but most natural ingredients are more sensitive and easily decomposed by light and heat, and have poor stability, thus losing their original functions. Microemulsion technology has good loading capacity for both hydrophilic and hydrophobic ingredients, good stability, and acts on the stratum corneum to enhance the fluidity of the lipid bilayer of the stratum corneum and weaken the skin barrier function, thereby effectively improving the transdermal penetration ability of substances. At the same time, microemulsions and nanoemulsions have good fluidity, adjustable viscosity, pleasant skin feel, and are easily accepted by consumers. Summary of the invention

[0006] In view of the deficiencies in the prior art, the object of the present invention is to provide a composition with a pore-refining effect, a nano-microemulsion and a preparation method and application thereof, and specifically to provide a composition with a pore-refining effect, a nano-microemulsion with a pore-refining effect and a preparation method thereof, and an essence milk with a pore-refining effect and a preparation method thereof.

[0007] In order to achieve the purpose of the invention, the present invention adopts the following technical solutions:

[0008] In a first aspect, the present invention provides a composition having a pore-refining effect, wherein the composition having a pore-refining effect comprises a Fucus vesiculosus extract, dipalmitoyl hydroxyproline and dipeptide diaminobutyryl benzylamide diacetate.

[0009] The present invention creatively finds that the composition composed of three ingredients, namely, Fucus vesiculosus extract, dipalmitoyl hydroxyproline and dipeptide diaminobutyryl benzylamide diacetate, can interact with each other and have a positive influence on each other, wherein the Fucus vesiculosus extract balances skin oil and shrinks pores from the root; wherein dipalmitoyl hydroxyproline is an important raw material for synthesizing collagen and supplements collagen to support pores; wherein dipeptide diaminobutyryl benzylamide diacetate can smooth the skin, and the three ingredients have a synergistic promotion effect in the effect of fine pores that is difficult for technicians in this field to predict, and more effectively smooth the skin and shrink pores. The composition is very suitable for use in skin care products, is mild and non-irritating, suitable for sensitive skin, and has good stability.

[0010] Preferably, the composition having the effect of refining pores comprises, by weight, 5-20 parts of Fucus vesiculosus extract, 2-10 parts of dipalmitoyl hydroxyproline and 2-10 parts of dipeptide diaminobutyryl benzylamide diacetate.

[0011] When the above three components are combined in a specific mass ratio, they have a more significant mutually reinforcing effect in refining pores.

[0012] The mass proportion of the Fucus vesiculosus extract can be selected from 5 parts, 7 parts, 8 parts, 10 parts, 12 parts, 14 parts, 16 parts, 18 parts, 20 parts, etc.

[0013] The mass fraction of the dipalmitoyl hydroxyproline can be selected from 2 parts, 3 parts, 4 parts, 5 parts, 6 parts, 7 parts, 8 parts, 9 parts, 10 parts, etc.

[0014] The mass fraction of the dipeptide diaminobutyryl benzylamide diacetate can be selected from 2 parts, 3 parts, 4 parts, 5 parts, 6 parts, 7 parts, 8 parts, 9 parts, 10 parts, etc.

[0015] Other specific point values ​​within the above numerical ranges can be selected and will not be described in detail here.

[0016] In a second aspect, the present invention provides a nano-microemulsion with pore-refining effect. The raw materials for preparing the nano-microemulsion with pore-refining effect include the composition with pore-refining effect described in the first aspect, a surfactant, a polyol and water.

[0017] The present invention creatively utilizes microemulsion technology to encapsulate the above composition, which can improve the stability of the composition, help the composition stay and penetrate in the pores, increase the permeability, enrich the sebaceous glands, and slowly release it for long-term effect.

[0018] Preferably, the raw materials for preparing the nano-microemulsion with pore-refining effect include, by mass, 10-30 parts of the composition with pore-refining effect described in the first aspect, 15-30 parts of surfactants, 5-15 parts of polyols, and 30-70 parts of water.

[0019] When the composition, surfactant, polyol and water are combined in the above-mentioned specific mass ratio, the nano-microemulsion system is more stable while ensuring the optimization of efficacy.

[0020] The mass parts of the composition can be selected from 10 parts, 12 parts, 14 parts, 16 parts, 18 parts, 20 parts, 22 parts, 24 parts, 26 parts, 28 parts, 30 parts, etc.

[0021] The mass fraction of the surfactant can be selected from 15 parts, 17 parts, 18 parts, 20 parts, 22 parts, 24 parts, 25 parts, 26 parts, 28 parts, 29 parts, 30 parts, etc.

[0022] The mass fraction of the polyol can be selected from 5 parts, 6 parts, 7 parts, 8 parts, 9 parts, 10 parts, 11 parts, 12 parts, 13 parts, 14 parts, 15 parts, etc.

[0023] The mass fraction of the water can be selected from 30 parts, 35 parts, 40 parts, 45 parts, 50 parts, 55 parts, 60 parts, 65 parts, 70 parts, etc.

[0024] Other specific point values ​​within the above numerical ranges can be selected and will not be described in detail here.

[0025] Preferably, the surfactant includes a nonionic surfactant and an anionic surfactant.

[0026] The nonionic surfactant is selected from any one of Tween, Span, polyglycerol oleate, castor oil polyoxyethylene ether or polyoxyethylene ether hydrogenated castor oil or a combination of at least two thereof.

[0027] The anionic surfactant is selected from any one of sodium stearoyl glutamate, sodium stearoyl lactylate, potassium cetyl phosphate or sodium N-stearoyl-N-methyl taurate, or a combination of at least two thereof.

[0028] Preferably, the surfactant includes Tween, Span and sodium stearoyl lactylate.

[0029] The surfactant in the nano-microemulsion product of the present invention is more preferably a combination of Tween, Span and sodium stearoyl lactylate because the surfactant in this combination can make the nano-microemulsion system more long-lasting and stable, thereby maintaining the stability and considerable permeability of the functional composition.

[0030] Preferably, the polyol includes any one of glycerol, 1,3-butylene glycol, polyethylene glycol, propylene glycol, hexylene glycol or polypropylene glycol, or a combination of at least two thereof.

[0031] In a third aspect, the present invention provides a method for preparing the nano-microemulsion having the effect of refining pores according to the second aspect, the preparation method comprising:

[0032] Dipalmitoylhydroxyproline and a surfactant are mixed and dissolved to obtain liquid A; Fucus vesiculosus extract, dipeptide diaminobutyryl benzylamide diacetate, polyol and water are mixed and dissolved to obtain liquid B; Liquid A and liquid B are mixed and homogenized to obtain the nano-microemulsion with pore-refining effect.

[0033] The preparation method of the nano-microemulsion involved in the present invention is simple and easy, and is very suitable for industrialized scale-up production. The droplets of the prepared nano-microemulsion are very small, which can overcome the unstable factors in some emulsion systems, improve the stability of the composition, help the composition stay and penetrate in the pores, improve the permeability, enrich the sebaceous glands, and slowly release, so as to take effect for a long time.

[0034] Preferably, the mixing and dissolving of dipalmitoyl hydroxyproline and a surfactant is carried out at 45-60°C, for example, 45°C, 50°C, 55°C, 60°C, etc.

[0035] Preferably, the mixing and dissolving of the Fucus vesiculosus extract, dipeptide diaminobutyryl benzylamide diacetate, polyol and water is carried out at 15-40°C, for example, 15°C, 20°C, 25°C, 30°C, 35°C, 40°C, etc.

[0036] Preferably, the mixing and homogenizing is carried out at 15-40°C (e.g., 15°C, 20°C, 25°C, 30°C, 35°C, 40°C, etc.) for 5-15 min (e.g., 5 min, 8 min, 10 min, 12 min, 15 min, etc.).

[0037] Other specific point values ​​within the above numerical ranges can be selected and will not be described in detail here.

[0038] For example, the preparation method of the nano-microemulsion having the effect of refining pores may be specifically as follows:

[0039] Heat dipalmitoyl hydroxyproline, Tween-65, sodium stearoyl lactylate and Span 80 to dissolve at 45-55°C, and vortex oscillate with a vortex oscillator until the raw materials are evenly mixed to obtain liquid A; separately dissolve fucus vesiculosus extract, dipeptide diaminobutyryl benzylamide diacetate and propylene glycol in water, and mix evenly at 15-40°C to obtain liquid B; mix liquid A and liquid B, and then oscillate them at 15-40°C for 5-15 minutes to obtain the product.

[0040] In a fourth aspect, the present invention provides use of the composition with pore-refining effect described in the first aspect or the nano-microemulsion with pore-refining effect described in the second aspect in the preparation of cosmetics.

[0041] The composition or nano-microemulsion with pore-refining effect of the present invention can be adapted to cosmetics with various functions and dosage forms, such as lotions, emulsions, facial masks, facial cleansing products, makeup removal products, etc. Other commonly used functional ingredients can be added to these cosmetics according to actual needs, such as whitening ingredients (niacinamide, glutathione, etc.), antioxidant ingredients (such as coenzyme Q10, vitamin E, tocopherol acetate, etc.), moisturizing ingredients (hyaluronic acid, sodium hyaluronate, hydrolyzed collagen, etc.).

[0042] In a fifth aspect, the present invention provides an essence milk with pore-refining effect, wherein the raw materials for preparing the essence milk with pore-refining effect include the nano-microemulsion with pore-refining effect described in the second aspect, an emulsifier, a thickener, a moisturizer, a pH adjuster and water.

[0043] Preferably, the raw materials for preparing the essence milk with the effect of refining pores also include any one of fillers, preservatives, fragrances, and antioxidants, or a combination of at least two of them.

[0044] The filler is for example diatomaceous earth, titanium dioxide, etc. The preservative is for example sodium benzoate, phenoxyethanol, etc. The fragrance is for example various flavors, etc. The pH adjuster is for example lactic acid, arginine, etc. The antioxidant is for example tocopherol ethyl acetate, etc.

[0045] Preferably, the emollient comprises any one of glycerin, butylene glycol, squalane, ethyl nonanoate, caprylic capric triglyceride, dimethicone or stearyl alcohol, or a combination of at least two thereof.

[0046] Preferably, the thickener includes any one or a combination of at least two of carbomer, hydroxyethyl cellulose, taro gum, sclerotium gum, pectin, ammonium acryloyldimethyltaurate / beheneth-25 methacrylate crosspolymer or acrylic acid (ester) / C10-30 alkyl acrylate crosspolymer.

[0047] Preferably, the emulsifier includes any one of dipalmitin, glyceryl stearate, C12-20 alkyl glucoside, coconut glucoside, sucrose stearate or hydrogenated lecithin, or a combination of at least two thereof.

[0048] In a sixth aspect, the present invention provides a method for preparing the essence milk having the effect of refining pores according to the fifth aspect, the preparation method comprising:

[0049] The thickener is dispersed in water, then mixed with the emollient and the pH regulator for homogeneity, then mixed with the emulsifier for emulsification, and then mixed with the nano-microemulsion to obtain the essence milk with the effect of refining pores.

[0050] The preparation method of the essence milk involved in the present invention is simple and easy to implement, and is very suitable for industrialized scale-up production.

[0051] Preferably, the raw materials for the homogenized mixing further include any one of fillers, preservatives, fragrances or antioxidants, or a combination of at least two thereof.

[0052] Compared with the prior art, the present invention has the following beneficial effects:

[0053] The present invention creatively finds that the composition composed of three ingredients, namely, Fucus vesiculosus extract, dipalmitoyl hydroxyproline and dipeptide diaminobutyryl benzylamide diacetate, can interact with each other and have a positive influence on each other, wherein the Fucus vesiculosus extract balances skin oil and shrinks pores from the root; wherein dipalmitoyl hydroxyproline is an important raw material for synthesizing collagen and supplements collagen to support pores; wherein dipeptide diaminobutyryl benzylamide diacetate can smooth the skin, and the three ingredients have a synergistic promotion effect in the effect of fine pores that is difficult for technicians in this field to predict, and more effectively smooth the skin and shrink pores. The composition is very suitable for use in skin care products, is mild and non-irritating, suitable for sensitive skin, and has good stability.

[0054] The present invention also creatively utilizes microemulsion technology to encapsulate the above composition to obtain a nano-microemulsion product, which can improve the stability of the composition, help the composition stay and penetrate in the pores, increase the permeability, enrich the sebaceous glands, and slowly release it for long-term effect.

[0055] The composition or nano-microemulsion with pore-refining effect of the present invention can be adapted to cosmetics with various effects and various dosage forms, and is mild, non-irritating and has good stability. BRIEF DESCRIPTION OF THE DRAWINGS

[0056] Figure 1 This is a statistical diagram of the effect of the sample in Application Example 1 on the characteristic count of skin pores;

[0057] Figure 2 This is a statistical chart showing the effect of the sample in Application Example 1 on the skin pore score;

[0058] Figure 3 This is a statistical chart showing the effect of the sample in Application Example 1 on the percentile of skin pores;

[0059] Figure 4 This is a statistical chart showing the effect of the sample in Application Example 1 on the number of skin pores;

[0060] Figure 5 This is a statistical chart showing the effect of the sample in Application Example 1 on the average area of ​​skin pores;

[0061] Figure 6 This is a statistical chart showing the effect of the sample in Application Example 1 on the skin pore area ratio;

[0062] Figure 7 This is a picture of the improvement of facial pores of the sample test subject in Application Example 1 before and after using the sample. DETAILED DESCRIPTION

[0063] In order to further explain the technical means and effects adopted by the present invention, the technical solution of the present invention is further described below in conjunction with the preferred embodiments of the present invention, but the present invention is not limited to the scope of the embodiments.

[0064] The sources of the functional ingredients contained in the products involved in the following embodiments, comparative examples, application examples, and comparative application examples are as follows (only the functional ingredients are reflected, and the necessary auxiliary ingredients contained in other commercially available raw materials are not repeated):

[0065] Dipalmitoyl hydroxyproline was derived from a product purchased from Kangdisi Chemical (Hubei) Co., Ltd. under the trade name of Dipalmitoyl hydroxyproline;

[0066] The extract of Fucus vesiculosus was purchased from Guangzhou Jijia Trading Co., Ltd. under the trade name of Fucus vesiculosus extract;

[0067] Dipeptide diaminobutyryl benzylamide diacetate was derived from a product purchased from Wuhan Demeikai Biotechnology Co., Ltd. under the trade name of Dipeptide diaminobutyryl benzylamide diacetate;

[0068] Acrylates / C10-30 Alkyl Acrylate Crosspolymer was obtained from Guangzhou Hongcheng Biotechnology Co., Ltd. under the trade name of Acrylates / C10-30 Alkyl Acrylate Crosspolymer;

[0069] Hydroxyethyl cellulose was obtained from Shanghai Youpeng Chemical Co., Ltd. under the trade name of hydroxyethyl cellulose;

[0070] Dimethicone was derived from a product purchased from Nanjing Bermuda Biotechnology Co., Ltd. under the trade name Dimethicone;

[0071] Caprylic-capric triglyceride was obtained from Shanghai Yuanxi Biotechnology Co., Ltd. under the trade name of Caprylic-capric triglyceride.

[0072] Example 1

[0073] This embodiment provides a nano-microemulsion with the effect of refining pores, and the preparation method thereof is as follows:

[0074] (1) Mix and dissolve 5 parts of dipalmitoyl hydroxyproline, 12 parts of Tween-65, 8 parts of sodium stearoyl lactylate, and 3 parts of Span 80 at 50° C., and vortex and oscillate with a vortex oscillator until the raw materials are evenly mixed to obtain liquid A;

[0075] (2) 10 parts of Fucus vesiculosus extract, 5 parts of dipeptide diaminobutyryl benzylamide diacetate, 9 parts of propylene glycol and 48 parts of water were mixed and dissolved at 35° C. to obtain liquid B;

[0076] (3) Liquid A and Liquid B are mixed, and turbine-oscillated at 35° C. for 10 min to obtain the nano-microemulsion having the effect of refining pores.

[0077] Example 2

[0078] This embodiment provides a nano-microemulsion with the effect of refining pores, and the preparation method thereof is as follows:

[0079] (1) Mix and dissolve 2 parts of dipalmitoyl hydroxyproline, 8 parts of Tween-80, 10 parts of sodium stearoyl lactylate, and 5 parts of Span 80 at 50° C., and vortex and oscillate with a vortex oscillator until the raw materials are evenly mixed to obtain liquid A;

[0080] (2) 15 parts of Fucus vesiculosus extract, 3 parts of dipeptide diaminobutyryl benzylamide diacetate, 12 parts of 1,3-butanediol and 45 parts of water were mixed and dissolved at 35° C. to obtain liquid B;

[0081] (3) Liquid A and Liquid B are mixed, and turbine-oscillated at 35° C. for 10 min to obtain the nano-microemulsion having the effect of refining pores.

[0082] Example 3

[0083] This embodiment provides a nano-microemulsion with the effect of refining pores, and the preparation method thereof is as follows:

[0084] (1) 8 parts of dipalmitoyl hydroxyproline, 12 parts of Tween-65, 8 parts of sodium stearoyl glutamate, and 3 parts of Span 80 are mixed and dissolved at 50° C. After dissolution, vortex oscillation is performed using a vortex oscillator until the raw materials are uniformly mixed to obtain liquid A;

[0085] (2) 8 parts of Fucus vesiculosus extract, 4 parts of dipeptide diaminobutyryl benzylamide diacetate, 10 parts of hexylene glycol and 47 parts of water were mixed and dissolved at 35° C. to obtain liquid B;

[0086] (3) Liquid A and Liquid B are mixed, and turbine-oscillated at 35° C. for 10 min to obtain the nano-microemulsion having the effect of refining pores.

[0087] Example 4

[0088] This embodiment provides a nano-microemulsion with the effect of refining pores. The difference between the preparation method and the embodiment 1 is that the formula of the surfactant is different, and 12 parts of Tween-65, 8 parts of sodium stearoyl lactylate, and 3 parts of Span 80 are replaced by 15 parts of Tween-65 and 8 parts of sodium stearoyl lactylate. The other components remain unchanged, and the preparation method thereof refers to the embodiment 1.

[0089] Example 5

[0090] This embodiment provides a nano-microemulsion with the effect of refining pores. The difference between the preparation method and the embodiment 1 is that the formula of the surfactant is different, and 12 parts of Tween-65, 8 parts of sodium stearoyl lactylate, and 3 parts of Span 80 are replaced with 15 parts of Span 80 and 8 parts of sodium stearoyl lactylate. The other components remain unchanged, and the preparation method thereof refers to the embodiment 1.

[0091] Example 6

[0092] This embodiment provides a nano-microemulsion with the effect of refining pores. The difference between the preparation method and the embodiment 1 is that the formula of the surfactant is different, and 12 parts of Tween-65, 8 parts of sodium stearoyl lactylate, and 3 parts of Span 80 are replaced by 18 parts of Tween-65 and 5 parts of Span 80. The other components remain unchanged, and the preparation method thereof refers to the embodiment 1.

[0093] Comparative Example 1

[0094] This comparative example provides a nano-microemulsion, and the preparation method thereof is different from that of Example 1 only in that the preparation raw material does not contain dipalmitoyl hydroxyproline, and its mass fraction is distributed to the mass of the serrata extract and dipeptide diaminobutyryl benzylamide diacetate, specifically:

[0095] (1) 12 parts of Tween-65, 8 parts of sodium stearoyl lactylate, and 3 parts of Span 80 were mixed and dissolved at 50° C. After dissolution, the mixture was vortexed using a vortex oscillator until the raw materials were uniformly mixed to obtain liquid A;

[0096] (2) 13.5 parts of Fucus vesiculosus extract, 6.5 parts of dipeptide diaminobutyryl benzylamide diacetate, 9 parts of propylene glycol and 48 parts of water were mixed and dissolved at 35° C. to obtain liquid B;

[0097] (3) Liquid A and Liquid B are mixed, and turbine-oscillated at 35° C. for 10 min to obtain the nano-microemulsion having the effect of refining pores.

[0098] Comparative Example 2

[0099] This comparative example provides a nano-microemulsion, and the preparation method thereof is different from that of Example 1 only in that the raw material does not contain the extract of Fucus vesiculosus, and its mass fraction is distributed to the mass of dipalmitoyl hydroxyproline and dipeptide diaminobutyryl benzylamide diacetate, specifically:

[0100] (1) 10 parts of dipalmitoyl hydroxyproline, 12 parts of Tween-65, 8 parts of sodium stearoyl lactylate, and 3 parts of Span 80 were mixed and dissolved at 50° C. After dissolution, the mixture was vortexed using a vortex oscillator until the raw materials were uniformly mixed to obtain liquid A;

[0101] (2) 10 parts of dipeptide diaminobutyryl benzylamide diacetate, 9 parts of propylene glycol and 48 parts of water were mixed and dissolved at 35° C. to obtain liquid B;

[0102] (3) Liquid A and Liquid B are mixed, and turbine-oscillated at 35° C. for 10 min to obtain the nano-microemulsion having the effect of refining pores.

[0103] Comparative Example 3

[0104] This comparative example provides a nano-microemulsion, and the preparation method thereof is different from that of Example 1 only in that the raw material for preparation does not contain dipeptide diaminobutyryl benzylamide diacetate, and its mass fraction is distributed to the mass of dipalmitoyl hydroxyproline and Fucus serrata extract, specifically:

[0105] (1) Mix and dissolve 6.5 parts of dipalmitoyl hydroxyproline, 12 parts of Tween-65, 8 parts of sodium stearoyl lactylate, and 3 parts of Span 80 at 50° C., and vortex and oscillate with a vortex oscillator until the raw materials are uniformly mixed to obtain liquid A;

[0106] (2) 13.5 parts of Fucus vesiculosus extract, 9 parts of propylene glycol and 48 parts of water were mixed and dissolved at 35° C. to obtain liquid B;

[0107] (3) Liquid A and Liquid B are mixed, and turbine-oscillated at 35° C. for 10 min to obtain the nano-microemulsion having the effect of refining pores.

[0108] Application Example 1

[0109] This application example provides an essence lotion with the effect of refining pores, and its formula is as follows:

[0110]

[0111] Its preparation method is:

[0112] The thickener is completely moistened and dispersed in water to obtain a mixed solution; then an emollient, a filler and a pH adjuster are added to the mixed solution, heated to 65°C, and homogenized at 3000 r / min for 3 minutes to completely disperse it; then an emulsifier is added thereto, homogenized at 3000 r / min for 3 minutes to completely emulsify it, stirred and cooled to 30°C, and a nano-microemulsion is added to obtain the mixed solution.

[0113] Application Example 2-6

[0114] This application example provides five kinds of essence milks with the effect of refining pores. The difference between the formulas of the essence milks and the application example 1 is that the nano-microemulsion of the embodiment 1 is replaced by the nano-microemulsions of the embodiments 2 to 6 in equal amounts. The other components remain unchanged, and the preparation method thereof is referred to the application example 1.

[0115] Comparative Application Examples 1-3

[0116] This comparative application example provides three kinds of essence milks, the formula of which is different from that of application example 1 only in that the nano-microemulsion of embodiment 1 is replaced by the nano-microemulsion of comparative examples 1 to 3 in equal amounts. The other components remain unchanged, and the preparation method thereof is referred to application example 1.

[0117] Comparative Application Example 4

[0118] This comparative application example provides an emulsion, the formula of which differs from that of application example 1 only in that it does not contain the nano-microemulsion of embodiment 1, and the reduced amount thereof is supplemented by water.

[0119] Test Example 1

[0120] Skin safety test:

[0121] The products prepared in Application Examples 1-6 were placed in a patch tester, and the patch tester with the test substance added was attached to the flexor side of the forearm of the test persons (33 people), and the patch was lightly pressed with the palm to evenly apply it to the skin for 24 hours; the skin irritation and sensitization were observed according to Table 1 (Skin Reaction Grading Standard for Skin Closure Patch Test) 30 minutes, 24 hours, and 48 hours after the removal of the test substance patch tester, and the observation results were recorded.

[0122] Table 1

[0123]

[0124]

[0125] The results showed that the products of Application Examples 1-6 scored 0 at 30 minutes, 24 hours and 48 hours, and no adverse skin reactions occurred, indicating that the composition, nano-microemulsion and essence milk products involved in the present invention are safe and non-irritating to the skin.

[0126] Test Example 2

[0127] Pore ​​improvement effect evaluation:

[0128] The subjects were 90 Chinese healthy sensitive skin women aged between 27 and 48 years old, with an average age of 36.90±5.24 years old. They were randomly divided into 9 groups, with 10 people in each group, and used the products of application examples 1-6 and comparative application examples 1-3 respectively. The subjects gave written consent with knowledge. The subjects were willing to cooperate and understand the necessity and duration of control in order to comply with the plan formulated by the clinical trial center. A half-face experiment was carried out in comparison with the blank group (comparative application example 4). The products were used once in the morning and evening every day, and the method and amount of use were kept consistent. They were used for 28 consecutive days. The experimenters took VISIA photos and Primos CR pictures on D0, D14 and D28, and the subjects filled in the self-assessment questionnaire. The data were statistically analyzed using SPSS 28.0, and the test data were tested for normal distribution. If the test data was normally distributed, the T test method was used for statistical analysis; if the test data was non-normally distributed, the rank sum test method was used for analysis and statistics. The rank sum test was used for statistical analysis of graded data. The statistical method significance level p < 0.05.

[0129] (1) Skin pores (VISIA) (cheeks):

[0130] VISIA was used to collect facial image information and analyze photos. Feature count means counting feature parameters without calculating the size and strength of feature parameters. A smaller value indicates better skin. Score means evaluating the size, area, and strength of feature parameters. A smaller score indicates better skin. The statistical results are shown in Table 2, where the statistical graphs of the effects of the sample in Application Example 1 on skin pore feature count, skin pore score, and skin pore percentile are shown in Table 2. Figure 1-3 shown.

[0131] Table 2

[0132]

[0133] From the data in Table 2, it can be seen that compared with before using the sample, after using the essence lotion of Application Example 1 for 14 days, the characteristic count of skin pores on the cheeks decreased by 3.26%, the skin pore score decreased by 6.11%, and the skin pore percentile decreased by 2.20%; after using the sample for 28 days, the characteristic count of skin pores on the cheeks decreased by 3.93%, the skin pore score decreased by 13.40%, and the skin pore percentile decreased by 7.98% without significant difference. The effect is significantly better than that of the comparative application example, indicating that the extract of Fucus serrata, dipalmitoylhydroxyproline and dipeptide diaminobutyrylbenzylamide diacetate have a synergistic promotion effect in the effect of refining pores that is difficult for those skilled in the art to predict, and more effectively smooth the skin and shrink pores.

[0134] (2) Skin pores (VISIA & IPP) (cheeks)

[0135] VISIA is used to collect facial images and combined with Image-pro skin comprehensive analysis software for photo analysis. The skin pore analysis value becomes smaller, indicating that the skin pores have improved. The statistical results are shown in Table 3 (average decrease rate of 10 people in each group). The statistical graphs of the effects of the sample in Application Example 1 on the number of skin pores, the average area of ​​skin pores, and the proportion of skin pore area are shown in Table 3. Figure 4-6 shown.

[0136] Table 3

[0137]

[0138] It can be seen from the data in Table 3 that the nano-microemulsion involved in the present invention and the essence milk prepared therefrom have excellent pore-refining effect, and through the data of comparative application example 1 and comparative application examples 1-3, it can be seen that the three ingredients of Fucus vesiculosus extract, dipalmitoyl hydroxyproline and dipeptide diaminobutyryl benzylamide diacetate have a synergistic promoting effect in improving the effect of pores.

[0139] (3) A subject of Application Example 1 was randomly selected to observe and compare the pore conditions on the face of the subject on the 0th, 14th, and 28th days of the experiment. Figure 7 As shown in the figure, the black spots are marked pores. The left side of the face uses the sample of Application Example 1, and the right side of the face uses the blank group sample. After 14 days of use, the black marks on the left side of the face can be seen to be reduced, and after 28 days of use, it can be seen that they have become significantly less; there is no obvious change on the right side of the face.

[0140] Test Example 3

[0141] Stability test:

[0142] (1) Centrifugation experiment: The samples of Examples 1-6 were centrifuged at 25° C. and 3000 r / min for 30 min to observe whether the samples showed stratification.

[0143] (2) Thermal stability test: The samples of Examples 1-6 were placed in a constant temperature environment of (50±1)°C for 30 days and the phenomena of the samples were observed.

[0144] The results are shown in Table 4:

[0145] Table 4

[0146] Group Centrifuge test Thermal stability test Example 1 normal No abnormality Example 2 normal No abnormality Example 3 normal No abnormality Example 4 There is precipitation, which can be evenly dispersed by shaking There is precipitation, which can be evenly dispersed by shaking Example 5 There is precipitation, which can be evenly dispersed by shaking There is precipitation, which can be evenly dispersed by shaking Example 6 There is precipitation, which can be evenly dispersed by shaking There is precipitation, which can be evenly dispersed by shaking

[0147] From the results in Table 4, it can be seen that the nano-microemulsion and the essence milk prepared therefrom of the present invention have good stability through the optimization selection of surfactants, and there are no abnormalities in the centrifugation test and the thermal stability test, which can make the nano-microemulsion system more long-lasting and stable, thereby maintaining the stability and considerable permeability of the functional composition.

[0148] The applicant declares that the present invention uses the above-mentioned embodiments to illustrate a composition with pore-refining effect, nano-microemulsion and its preparation method and application, but the present invention is not limited to the above-mentioned embodiments, that is, it does not mean that the present invention must rely on the above-mentioned embodiments to be implemented. Those skilled in the art should understand that any improvement of the present invention, equivalent replacement of various raw materials of the product of the present invention, addition of auxiliary components, selection of specific methods, etc., all fall within the protection scope and disclosure scope of the present invention.

[0149] The preferred embodiments of the present invention are described in detail above. However, the present invention is not limited to the specific details in the above embodiments. Within the technical concept of the present invention, a variety of simple modifications can be made to the technical solution of the present invention, and these simple modifications all belong to the protection scope of the present invention.

[0150] It should also be noted that the various specific technical features described in the above specific embodiments can be combined in any suitable manner without contradiction. In order to avoid unnecessary repetition, the present invention will not further describe various possible combinations.

Claims

1. A nano-microemulsion with pore-refining effect, characterized in that: The raw materials for preparing the nano-microemulsion with the effect of refining pores include extract of Fucus vesiculosus, dipalmitoyl hydroxyproline, dipeptide diaminobutyryl benzylamide diacetate, surfactant, polyol and water; The scaly Fucus vesiculosus extract, dipalmitoyl hydroxyproline, and dipeptide diaminobutyryl benzylamide diacetate form a composition with the effect of refining pores; The surfactants include Tween, Span and sodium stearoyl lactylate; The nano-microemulsion is prepared by a method comprising the following steps: Dipalmitoylhydroxyproline and a surfactant are mixed and dissolved to obtain liquid A; Fucus vesiculosus extract, dipeptide diaminobutyryl benzylamide diacetate, polyol and water are mixed and dissolved to obtain liquid B; and liquid A and liquid B are mixed and homogenized to obtain the nano-microemulsion.

2. The nano-microemulsion with pore-refining effect according to claim 1, characterized in that: The raw materials for preparing the nano-microemulsion with pore-refining effect include, by weight, 10-30 parts of a composition with pore-refining effect, 15-30 parts of a surfactant, 5-15 parts of a polyol, and 30-70 parts of water; The composition with pore-refining effect comprises, by weight, 5-20 parts of Fucus vesiculosus extract, 2-10 parts of dipalmitoyl hydroxyproline and 2-10 parts of dipeptide diaminobutyryl benzylamide diacetate.

3. The nano-microemulsion with pore-refining effect according to claim 1, characterized in that: The polyol includes any one of glycerol, 1,3-butylene glycol, polyethylene glycol, propylene glycol, hexylene glycol or polypropylene glycol, or a combination of at least two thereof.

4. The nano-microemulsion with pore-refining effect according to claim 1, characterized in that: The step of mixing and dissolving the dipalmitoyl hydroxyproline and the surfactant is carried out at 45-60°C.

5. The nano-microemulsion with pore-refining effect according to claim 1, characterized in that: The process of mixing and dissolving the Fucus vesiculosus extract, dipeptide diaminobutyryl benzylamide diacetate, polyol and water is carried out at 15-40°C.

6. The nano-microemulsion with pore-refining effect according to claim 1, characterized in that: The mixing and homogenization is carried out at 15-40° C. for 5-15 min.

7. Use of the nano-microemulsion with pore-refining effect according to any one of claims 1 to 6 in the preparation of cosmetics.

8. An essence milk with the effect of refining pores, characterized in that: The raw materials for preparing the essence milk with pore-refining effect include the nano-microemulsion with pore-refining effect as claimed in any one of claims 1 to 6, an emulsifier, a thickener, a moisturizer, a pH regulator and water.

9. The essence milk with pore-refining effect according to claim 8, characterized in that: The raw materials for preparing the essence milk with the effect of refining pores also include any one of fillers, preservatives, fragrances, and antioxidants, or a combination of at least two of them.

10. The essence milk with pore-refining effect according to claim 8, characterized in that: The emollient includes any one of glycerin, butylene glycol, squalane, ethyl nonanoate, caprylic capric triglyceride, dimethicone or stearyl alcohol, or a combination of at least two thereof.

11. The essence milk with pore-refining effect according to claim 8, characterized in that: The thickener includes any one or a combination of at least two of carbomer, hydroxyethyl cellulose, taro gum, sclerotium gum, pectin, ammonium acryloyldimethyltaurate / beheneth-25 methacrylate crosspolymer or acrylic acid (ester) / C10-30 alkyl acrylate crosspolymer.

12. The essence milk with pore-refining effect according to claim 8, characterized in that: The emulsifier includes any one of dipalmitin, glyceryl stearate, C12-20 alkyl glucoside, coconut glucoside, sucrose stearate or hydrogenated lecithin, or a combination of at least two thereof.

13. The method for preparing the essence milk with pore-refining effect according to any one of claims 8 to 12, characterized in that: The preparation method comprises: The thickener is dispersed in water, then mixed with the emollient and the pH regulator for homogeneity, then mixed with the emulsifier for emulsification, and then mixed with the nano-microemulsion to obtain the essence milk with the effect of refining pores.

14. The method for preparing the essence milk with pore-refining effect according to claim 13, wherein the raw materials for the mixing and homogenization further include any one of fillers, preservatives, fragrances or antioxidants, or a combination of at least two thereof.

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