检测环状RNA和 / 或所述环状RNA表达量的制剂在制备结直肠癌诊断产品中的应用
By using circular RNAs hsa_circ_0005232 and hsa_circ_0042103 as molecular markers, combined with specific primers and quantitative real-time PCR, diagnostic reagents and kits for colorectal cancer were developed. This solved the problem of insufficient sensitivity of existing markers and enabled efficient screening and assessment of treatment efficacy for colorectal cancer.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- 刘丽媛
- Filing Date
- 2022-07-08
- Publication Date
- 2026-07-17
AI Technical Summary
Existing biomarkers for colorectal cancer detection lack sensitivity and specificity, and colonoscopy cannot be rapidly and effectively popularized, resulting in a lack of effective early prevention methods. At present, there are no reports on the application of circular RNAs hsa_circ_0005232 and hsa_circ_0042103 in colorectal cancer research.
Using circular RNAs hsa_circ_0005232 and/or hsa_circ_0042103 as novel molecular markers, specific primers P1, P2, P3, and P4 were designed to bind to the housekeeping gene GAPDH. The expression level of circular RNA was detected by real-time quantitative PCR, and diagnostic reagents and kits were developed for the auxiliary diagnosis and treatment efficacy assessment of colorectal cancer.
It provides a more accurate colorectal cancer screening tool, enabling simple and quick diagnosis, pathological grading, and clinical staging of colorectal cancer. It has good clinical application prospects, is economical and convenient, and is of great significance for further research on the biological mechanisms of colorectal cancer-related circular RNAs.
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Figure CN116004813B_ABST