A methacrylate porous layer open tubular capillary column and its preparation method and application

Abstract

The present invention belongs to the technical field of capillary columns. The present invention provides a method for preparing a methacrylate porous layer open tubular capillary column. 3-(trimethoxysilyl) propyl methacrylate and a methanol solution are mixed and injected into a capillary, and then sealed and placed to obtain a treated capillary; butyl methacrylate, 2-acrylamide-2-methyl-1-propanesulfonic acid, a cross-linking agent, a porogen and an initiator are ultrasonically mixed and deoxygenated to obtain a prepolymerization solution; the prepolymerization solution is injected into the treated capillary for polymerization reaction to obtain a methacrylate porous layer open tubular capillary column. The capillary of the present invention is modified with 3-(trimethoxysilyl) propyl methacrylate, and the method for obtaining a porous layer open tubular capillary column by in-situ thermal polymerization or photopolymerization is simple, the capillary column has a long service life, a high column capacity, and a good separation effect, and can be used in liquid chromatography separation technology, and is suitable for separation and analysis of deoxyribonucleic acid.

Description

Technical Field

[0001] The invention relates to the technical field of capillary columns, and in particular to a methacrylate porous layer open tubular capillary column and a preparation method and application thereof. Background Art

[0002] The open-tube capillary column was first proposed by Golay in 1957. He used a capillary column with a layer of stationary liquid on the inner wall instead of a packed column for experimentation and found that the hollow column had good permeability and far better separation performance than a packed column. Since the center of this column is empty and the inner wall of the tube is attached with a stationary liquid, it is called an open-tube capillary column.

[0003] Since the advent of open tubular capillary columns, they have continued to develop and have almost replaced packed columns in gas chromatography analysis applications, and have gradually been used in liquid chromatography. Open tubular columns can generally be divided into two categories. One is an open tubular column obtained by coating the inner wall of the tube with a stationary liquid, that is, a wall-coated open tubular column. Due to the small inner surface area of ​​the tube, the amount of stationary liquid coated is limited, and the capillary wall has poor wettability, it is not easy for the stationary liquid to form a uniform liquid film, resulting in poor repeatability and poor separation performance. In order to improve these deficiencies, it is usually necessary to first treat the surface of the inner wall of the tube to increase the surface roughness and improve the wettability, and then apply the stationary liquid; the other type is a porous layer open tubular column with a layer of porous material on the inner wall of the tube. The porous layer structure makes the column have a higher specific surface area and significantly improves the column capacity. The porous layer open tubular column can form a porous layer by chemically treating the inner wall of the capillary, obtain a porous stationary phase layer by deposition on the inner wall of the tube, or form a porous layer by polymerizing on the inner wall of the tube by chemical bonding. Among these preparation methods, the porous layer stationary phase is prepared by in-situ chemical bonding in the capillary, and its physical structure is easy to control, its surface chemical properties are easy to modify, and the stationary phase has good chemical stability.

[0004] So far, there are few reports on the preparation method and application of methacrylate porous layer open tubular capillary column, so it has good prospects to provide a methacrylate porous layer open tubular capillary column and its preparation method and application. Summary of the invention

[0005] The purpose of the present invention is to provide a methacrylate porous layer open tubular capillary column and a preparation method and application thereof in view of the deficiencies in the prior art.

[0006] In order to achieve the above-mentioned object of the invention, the present invention provides the following technical solutions:

[0007] The present invention provides a method for preparing a methacrylate porous layer open tubular capillary column, comprising the following steps:

[0008] 1) Mixing 3-(trimethoxysilyl)propyl methacrylate and a methanol solution, injecting the mixture into a capillary, and then sealing and placing the mixture to obtain a treated capillary;

[0009] 2) ultrasonically mixing butyl methacrylate, 2-acrylamide-2-methyl-1-propanesulfonic acid, a crosslinking agent, a porogen and an initiator and deoxygenating the mixture to obtain a prepolymerization solution;

[0010] 3) Injecting the prepolymerization solution into the treated capillary to carry out polymerization reaction, thereby obtaining a methacrylate porous layer open tubular capillary column.

[0011] Preferably, the mass fraction of the methanol solution in step 1) is 97-99.9%; the volume ratio of the 3-(trimethoxysilyl)propyl methacrylate to the methanol solution is 1:0.5-1.5.

[0012] Preferably, in step 1), the inner diameter of the capillary is 2-10 μm; the sealing temperature is 20-35° C., and the sealing time is 12-24 h.

[0013] Preferably, in step 2), the crosslinking agent is ethylene glycol dimethacrylate; the porogen comprises n-propanol, 1,4-butanediol and water; and the initiator is azobisisobutyronitrile or 2,2-dimethoxy-2-phenylacetophenone.

[0014] Preferably, in step 2), the mass ratio of butyl methacrylate, 2-acrylamide-2-methyl-1-propanesulfonic acid, crosslinking agent, porogen and initiator is 10-30:1-6:5-15:47-100:0.2-0.4; the mass ratio of n-propanol, 1,4-butanediol and water in the porogen is 30-60:12-32:5-8.

[0015] Preferably, the power of the ultrasonic mixing in step 2) is 90-110 W, and the time of the ultrasonic mixing is 4-6 min; and the polymerization reaction in step 3) is in-situ thermal polymerization or photopolymerization.

[0016] Preferably, the temperature of the in-situ thermal polymerization is 40-80° C., and the time of the in-situ thermal polymerization is 4-24 hours; the temperature of the photopolymerization is 18-26° C., and the time of the photopolymerization is 15-35 minutes.

[0017] The invention also provides a methacrylate porous layer open tubular capillary column prepared by the preparation method.

[0018] The invention also provides application of the methacrylate porous layer open tubular capillary column in open tubular capillary liquid chromatography separation.

[0019] Preferably, a methacrylate porous layer open tubular capillary column is used for separation and analysis of deoxyribonucleic acid.

[0020] The beneficial effects of the present invention include the following:

[0021] 1) The capillary of the present invention is modified on the inner wall of the capillary by 3-(trimethoxymethylsilyl)propyl methacrylate, and the method of obtaining the methacrylate porous layer open tubular capillary column by in-situ polymerization or photopolymerization in the capillary by thermal initiation is simple to operate, the polymer is combined with the inner wall of the capillary by covalent bonding, is not easy to fall off, and the capillary column has a long service life.

[0022] 2) The methacrylate porous layer open tubular capillary column of the present invention adopts in-situ thermal polymerization or photopolymerization to form a uniform organic polymer porous layer stationary phase in the capillary, which increases the specific surface area and the number of action sites, thereby improving the capacity and separation effect of the capillary column.

[0023] 3) The methacrylate porous layer open tubular capillary column provided by the present invention can be used in open tubular capillary liquid chromatography separation technology and is suitable for separation and analysis of deoxyribonucleic acid. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] Figure 1 This is a scanning electron microscope image of the methacrylate porous layer open tubular capillary column prepared in Example 1;

[0025] Figure 2 This is a diagram showing the separation and analysis of deoxyribonucleic acid using the methacrylate porous layer open tubular capillary column prepared in Example 1. DETAILED DESCRIPTION

[0026] The present invention provides a method for preparing a methacrylate porous layer open tubular capillary column, comprising the following steps:

[0027] 1) Mixing 3-(trimethoxysilyl)propyl methacrylate and a methanol solution, injecting the mixture into a capillary, and then sealing and placing the mixture to obtain a treated capillary;

[0028] 2) ultrasonically mixing butyl methacrylate, 2-acrylamide-2-methyl-1-propanesulfonic acid, a crosslinking agent, a porogen and an initiator and deoxygenating the mixture to obtain a prepolymerization solution;

[0029] 3) Injecting the prepolymerization solution into the treated capillary to carry out polymerization reaction, thereby obtaining a methacrylate porous layer open tubular capillary column.

[0030] In the present invention, the mass fraction of the methanol solution in step 1) is preferably 97-99.9%, more preferably 97.5-99%, and more preferably 98%; the volume ratio of the 3-(trimethoxysilyl)propyl methacrylate to the methanol solution is preferably 1:0.5-1.5, more preferably 1:0.8-1.2, and more preferably 1:1.

[0031] In the present invention, the inner diameter of the capillary in step 1) is preferably 2-10 μm, more preferably 4-8 μm, and more preferably 5-6 μm; the temperature of the sealed placement is preferably 20-35°C, more preferably 25-30°C, and more preferably 26-28°C; the sealed placement time is preferably 12-24h, more preferably 14-22h, and more preferably 16-20h.

[0032] In the present invention, the capillary in step 1) is preferably a capillary which is washed with sodium hydroxide solution, water, hydrochloric acid solution, water and methanol solution in sequence and then dried with nitrogen; the concentration of the sodium hydroxide solution is preferably 0.5-1.0 mol / L, more preferably 0.7-0.8 mol / L, and more preferably 0.75 mol / L; the water is preferably deionized water; the concentration of the hydrochloric acid solution is preferably 0.05-0.15 mol / L, more preferably 0.08-0.12 mol / L, and more preferably 0.1 mol / L; The mass fraction of the methanol solution is preferably 97-99.9%, more preferably 97.5-99%, and more preferably 98%; the rinsing time with the sodium hydroxide solution is preferably 1-3 hours, more preferably 1.5-2.5 hours, and more preferably 2 hours; each independent rinsing with water is preferably performed to rinse the capillary to neutrality; the rinsing time with the hydrochloric acid solution is preferably 0.5-1 hour, more preferably 0.7-0.9 hours, and more preferably 0.8 hours; the rinsing time with the methanol solution is preferably 1-3 hours, more preferably 1.5-2.5 hours, and more preferably 2 hours.

[0033] In the present invention, the capillary in step 1) is preferably a quartz capillary.

[0034] In the present invention, during the sealing step 1), the sealant used is preferably silica gel; preferably, both ends of the capillary are sealed.

[0035] In the present invention, after the sealed placement in step 1), the capillary is preferably rinsed with a methanol solution and blown dry with nitrogen in sequence; the mass fraction of the methanol solution is preferably 97 to 99.9%, more preferably 97.5 to 99%, and more preferably 98%; the rinsing time of the methanol solution is preferably 1 to 3 hours, more preferably 1.5 to 2.5 hours, and more preferably 2 hours.

[0036] In the present invention, the crosslinking agent in step 2) is preferably ethylene glycol dimethacrylate; the porogen preferably comprises n-propanol, 1,4-butanediol and water; and the initiator is preferably azobisisobutyronitrile or 2,2-dimethoxy-2-phenylacetophenone.

[0037] In the present invention, the water contained in the porogen is preferably deionized water.

[0038] In the present invention, the mass ratio of butyl methacrylate, 2-acrylamide-2-methyl-1-propanesulfonic acid, crosslinking agent, porogen and initiator in step 2) is preferably 10-30: 1-6: 5-15: 47-100: 0.2-0.4, further preferably 15-25: 2-5: 8-12: 67-80: 0.25-0.35, more preferably 18-22: 3-4: 9-11: 72-75: 0.3; the mass ratio of n-propanol, 1,4-butanediol and water in the porogen is preferably 30-60: 12-32: 5-8, further preferably 40-50: 20-24: 6-7, more preferably 44-46: 21-23: 6.4-6.6.

[0039] In the present invention, the power of the ultrasonic mixing in step 2) is preferably 90-110 W, more preferably 95-105 W, and more preferably 100 W; the time of ultrasonic mixing is preferably 4-6 min, more preferably 4.5-5.5 min, and more preferably 5 min; the polymerization reaction in step 3) is preferably in-situ thermal polymerization or photopolymerization.

[0040] In the present invention, the temperature of the in-situ thermal polymerization is preferably 40-80°C, more preferably 50-70°C, and more preferably 55-65°C; the time of the in-situ thermal polymerization is preferably 4-24h, more preferably 10-18h, and more preferably 12-16h; the temperature of the photopolymerization is preferably 18-26°C, more preferably 20-24°C, and more preferably 22°C; the time of the photopolymerization is preferably 15-35min, more preferably 20-30min, and more preferably 22-28min.

[0041] In the present invention, the in-situ thermal polymerization is preferably carried out in a water bath; and the photopolymerization is preferably carried out in a UV analyzer.

[0042] In the present invention, the deoxygenation method in step 2) is preferably nitrogen deoxygenation.

[0043] In the present invention, after the prepolymerization solution is injected into the treated capillary in step 3), both ends of the capillary are preferably sealed with silica gel.

[0044] In the present invention, after the polymerization reaction in step 3), the capillary is preferably rinsed with a methanol solution; the mass fraction of the methanol solution is preferably 97 to 99.9%, more preferably 97.5 to 99%, and more preferably 98%; the rinsing time of the methanol solution is preferably 7 to 9 hours, and more preferably 8 hours; the role of the methanol solution rinse is to remove unreacted reagents to obtain a methacrylate porous layer open tubular capillary column.

[0045] The invention also provides a methacrylate porous layer open tubular capillary column prepared by the preparation method.

[0046] The invention also provides application of the methacrylate porous layer open tubular capillary column in open tubular capillary liquid chromatography separation.

[0047] In the present invention, the methacrylate porous layer open tubular capillary column is preferably used for separation and analysis of deoxyribonucleic acid.

[0048] The technical solutions provided by the present invention are described in detail below in conjunction with the embodiments, but they should not be construed as limiting the protection scope of the present invention.

[0049] Example 1

[0050] A quartz capillary with an inner diameter of 2.6 μm was rinsed with a 1 mol / L sodium hydroxide solution for 2 h, rinsed with deionized water until neutral, rinsed with a 0.1 mol / L hydrochloric acid solution for 1 h, rinsed with deionized water until neutral, rinsed with a 99.9% methanol solution for 2 h, and then dried with nitrogen; 1 mL of 3-(trimethoxymethylsilyl)propyl methacrylate and 1 mL of 99.9% methanol solution were mixed and injected into the quartz capillary, and then both ends of the capillary were sealed with silica gel, placed at 20°C for 24 h, and then rinsed with a 99.9% methanol solution for 3 h and dried with nitrogen to obtain a treated quartz capillary.

[0051] 22.3g of butyl methacrylate, 1.1g of 2-acrylamide-2-methyl-1-propanesulfonic acid, 6.8g of ethylene glycol dimethacrylate, 69.2g of a porogen (containing 40.5g of n-propanol, 23g of 1,4-butanediol, and 5.7g of deionized water) and 0.3g of azobisisobutyronitrile were mixed for 4 minutes under an ultrasonic power of 90W, and nitrogen was introduced for deoxygenation to obtain a prepolymerization solution; the prepolymerization solution was injected into a treated quartz capillary, and both ends of the quartz capillary were sealed with silica gel, and then in-situ thermal polymerization was carried out in a water bath at a temperature of 60°C for 16 hours, and then rinsed with a methanol solution with a mass fraction of 99.9% for 7 hours to remove unreacted reagents to obtain a methacrylate porous layer open tubular capillary column.

[0052] The scanning electron microscope image of the methacrylate porous layer open tubular capillary column of this embodiment is magnified 30k times as shown in FIG. Figure 1 As shown. Figure 1 It can be seen that the methacrylate porous layer open tubular capillary column prepared in this example has a uniform porous layer structure.

[0053] The prepared methacrylate porous layer open tubular capillary column of this example was used in the chromatography mode, with 20.0 mmol / L tris(hydroxymethylaminomethane) hydrochloride (containing 0.5 mol / L sodium chloride solution) as the mobile phase, and the deoxyribonucleic acid was eluted at 300 psi. The obtained chromatogram is shown in FIG. Figure 2 As shown. Figure 2 It can be seen that the deoxyribonucleic acid fragments have been effectively separated, and the chromatographic peaks from left to right are 20kbp, 10kbp, 7kbp, 5kbp, 4kbp, 3kbp, 2kbp, 1.5kbp, 1kbp, 700bp, 500bp, 400bp, 300bp, 200bp, and 75bp.

[0054] Example 2

[0055] A quartz capillary with an inner diameter of 2 μm was rinsed with a 0.5 mol / L sodium hydroxide solution for 3 h, rinsed with deionized water until neutral, rinsed with a 0.05 mol / L hydrochloric acid solution for 0.8 h, rinsed with deionized water until neutral, rinsed with a 97% methanol solution for 3 h, and then dried with nitrogen; 1 mL of 3-(trimethoxymethylsilyl)propyl methacrylate and 0.5 mL of a 97% methanol solution were mixed and injected into the quartz capillary, and then both ends of the capillary were sealed with silica gel, placed at 35°C for 12 h, and then rinsed with a 97% methanol solution for 2 h and dried with nitrogen to obtain a treated quartz capillary.

[0056] 10g of butyl methacrylate, 1g of 2-acrylamide-2-methyl-1-propanesulfonic acid, 5g of ethylene glycol dimethacrylate, 47g of a porogen (including 30g of n-propanol, 12g of 1,4-butanediol, and 5g of deionized water) and 0.2g of azobisisobutyronitrile were mixed under an ultrasonic power of 110W for 6 minutes, and nitrogen was introduced for deoxygenation to obtain a prepolymerization solution; the prepolymerization solution was injected into the treated quartz capillary, and both ends of the quartz capillary were sealed with silica gel, and then in-situ thermal polymerization was carried out in a water bath at a temperature of 40°C for 4h, and then rinsed with a methanol solution with a mass fraction of 97% for 8h to remove unreacted reagents to obtain a methacrylate porous layer open tubular capillary column.

[0057] Example 3

[0058] A quartz capillary with an inner diameter of 10 μm was rinsed with a 0.8 mol / L sodium hydroxide solution for 1 hour, rinsed with deionized water until neutral, rinsed with a 0.15 mol / L hydrochloric acid solution for 0.5 hour, rinsed with deionized water until neutral, rinsed with a 98.5% methanol solution for 1 hour, and then dried with nitrogen; 1 mL of 3-(trimethoxymethylsilyl)propyl methacrylate and 1.5 mL of a 98.5% methanol solution were mixed and injected into the quartz capillary, and then both ends of the capillary were sealed with silica gel, placed at 25° C. for 18 hours, and then rinsed with a 98.5% methanol solution for 1 hour and dried with nitrogen to obtain a treated quartz capillary.

[0059] 30g of butyl methacrylate, 6g of 2-acrylamide-2-methyl-1-propanesulfonic acid, 15g of ethylene glycol dimethacrylate, 100g of a porogen (including 60g of n-propanol, 32g of 1,4-butanediol, and 8g of deionized water) and 0.4g of azobisisobutyronitrile were mixed for 5 minutes under an ultrasonic power of 100W, and nitrogen was introduced for deoxygenation to obtain a prepolymerization solution; the prepolymerization solution was injected into a treated quartz capillary, and both ends of the quartz capillary were sealed with silica gel, and then in-situ thermal polymerization was carried out in a water bath at a temperature of 80°C for 24 hours, and then rinsed with a methanol solution with a mass fraction of 98.5% for 9 hours to remove unreacted reagents to obtain a methacrylate porous layer open tubular capillary column.

[0060] Example 4

[0061] A quartz capillary with an inner diameter of 5 μm was rinsed with a 0.8 mol / L sodium hydroxide solution for 2.5 h, rinsed with deionized water until neutral, rinsed with a 0.12 mol / L hydrochloric acid solution for 1 h, rinsed with deionized water until neutral, rinsed with a 99.9% methanol solution for 2 h, and then dried with nitrogen; 1 mL of 3-(trimethoxymethylsilyl)propyl methacrylate and 0.5 mL of a 99.9% methanol solution were mixed and injected into the quartz capillary, and then both ends of the capillary were sealed with silica gel, placed at 35° C. for 12 h, and then rinsed with a 99.9% methanol solution for 1 h and then dried with nitrogen to obtain a treated quartz capillary.

[0062] 10g of butyl methacrylate, 1g of 2-acrylamide-2-methyl-1-propanesulfonic acid, 5g of ethylene glycol dimethacrylate, 47g of a porogen (including 30g of n-propanol, 12g of 1,4-butanediol, and 5g of deionized water) and 0.2g of 2,2-dimethoxy-2-phenylacetophenone were mixed for 6 minutes under an ultrasonic power of 110W, and nitrogen was introduced for deoxygenation to obtain a prepolymerization solution; the prepolymerization solution was injected into a treated quartz capillary, and both ends of the quartz capillary were sealed with silica gel, and then photopolymerization was carried out in an ultraviolet analyzer at a temperature of 18°C ​​for 15 minutes, and then rinsed with a methanol solution with a mass fraction of 99.9% for 7.5 hours to remove unreacted reagents to obtain a methacrylate porous layer open tubular capillary column.

[0063] Example 5

[0064] A quartz capillary with an inner diameter of 2 μm was rinsed with a 1 mol / L sodium hydroxide solution for 3 h, rinsed with deionized water until neutral, rinsed with a 0.15 mol / L hydrochloric acid solution for 0.5 h, rinsed with deionized water until neutral, rinsed with a 97% methanol solution for 3 h, and then dried with nitrogen; 1 mL of 3-(trimethoxymethylsilyl)propyl methacrylate and 0.8 mL of a 97% methanol solution were mixed and injected into the quartz capillary, and then both ends of the capillary were sealed with silica gel, placed at 20°C for 15 h, and then rinsed with a 97% methanol solution for 3 h and dried with nitrogen to obtain a treated quartz capillary.

[0065] 25g of butyl methacrylate, 4g of 2-acrylamide-2-methyl-1-propanesulfonic acid, 8g of ethylene glycol dimethacrylate, 59g of a porogen (including 33g of n-propanol, 18g of 1,4-butanediol, and 8g of deionized water) and 0.3g of 2,2-dimethoxy-2-phenylacetophenone were mixed for 4 minutes under an ultrasonic power of 100W, and nitrogen was introduced for deoxygenation to obtain a prepolymerization solution; the prepolymerization solution was injected into a treated quartz capillary, and both ends of the quartz capillary were sealed with silica gel, and then photopolymerization was carried out in an ultraviolet analyzer at a temperature of 24°C for 35 minutes, and then rinsed with a methanol solution with a mass fraction of 97% for 8 hours to remove unreacted reagents to obtain a methacrylate porous layer open tubular capillary column.

[0066] Example 6

[0067] A quartz capillary with an inner diameter of 10 μm was rinsed with a 0.5 mol / L sodium hydroxide solution for 1 hour, rinsed with deionized water until neutral, rinsed with a 0.05 mol / L hydrochloric acid solution for 0.7 hour, rinsed with deionized water until neutral, rinsed with a 98% methanol solution for 1 hour, and then dried with nitrogen; 1 mL of 3-(trimethoxymethylsilyl)propyl methacrylate and 1.5 mL of a 98% methanol solution were mixed and injected into the quartz capillary, and then both ends of the capillary were sealed with silica gel, placed at 24°C for 24 hours, and then rinsed with a 98% methanol solution for 2.5 hours and dried with nitrogen to obtain a treated quartz capillary.

[0068] 30g of butyl methacrylate, 6g of 2-acrylamide-2-methyl-1-propanesulfonic acid, 15g of ethylene glycol dimethacrylate, 100g of a porogen (comprising 60g of n-propanol, 32g of 1,4-butanediol and 8g of deionized water) and 0.4g of 2,2-dimethoxy-2-phenylacetophenone were mixed for 5min under an ultrasonic power of 90W, and nitrogen was introduced for deoxygenation to obtain a prepolymerization solution; the prepolymerization solution was injected into a treated quartz capillary, and both ends of the quartz capillary were sealed with silica gel, and then photopolymerization was carried out in an ultraviolet analyzer at a temperature of 26°C for 26min, and then the unreacted reagents were removed by washing with a methanol solution with a mass fraction of 98% for 7h to obtain a methacrylate porous layer open tubular capillary column.

[0069] The capillary of the invention adopts 3-(trimethoxysilyl)propyl methacrylate to modify the inner wall of the capillary. The method for obtaining the methacrylate porous layer open tubular capillary column by in-situ thermal polymerization or photopolymerization in the capillary is simple to operate. The polymer is combined with the inner wall of the capillary through covalent bonding, is not easy to fall off, and has a long column life. A uniform organic polymer porous layer stationary phase is formed in the capillary, the specific surface area is increased, the number of action sites is increased, the column capacity and the separation effect are improved, and the capillary can be used in the open tubular capillary liquid chromatography separation technology, and is suitable for the separation and analysis of deoxyribonucleic acid.

[0070] The above is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principle of the present invention. These improvements and modifications should also be regarded as the scope of protection of the present invention.

Claims

1. Application of a methacrylate porous layer open tubular capillary column in open tubular capillary liquid chromatography separation, It is characterized in that The methacrylate porous layer open tubular capillary column was used for the separation and analysis of deoxyribonucleic acid. The preparation method of the methacrylate porous layer open tubular capillary column comprises the following steps: 1) Mixing 3-(trimethoxysilyl)propyl methacrylate and a methanol solution, injecting the mixture into a capillary, and then sealing and placing the mixture to obtain a treated capillary; 2) ultrasonically mixing butyl methacrylate, 2-acrylamide-2-methyl-1-propanesulfonic acid, a crosslinking agent, a porogen and an initiator and deoxygenating the mixture to obtain a prepolymerization solution; 3) injecting the prepolymerization solution into the treated capillary to carry out polymerization reaction, thereby obtaining a methacrylate porous layer open tubular capillary column; Step 1) The inner diameter of the capillary is 2 to 10 μm; Step 2) the crosslinking agent is ethylene glycol dimethacrylate; the porogen comprises n-propanol, 1,4-butanediol and water; Step 2) The mass ratio of butyl methacrylate, 2-acrylamide-2-methyl-1-propanesulfonic acid, crosslinking agent, porogen and initiator is 10-30:1-6:5-15:47-100:0.2-0.4; the mass ratio of n-propanol, 1,4-butanediol and water in the porogen is 30-60:12-32:5-8; The polymerization reaction in step 3) is in-situ thermal polymerization or photopolymerization.

2. The use according to claim 1, It is characterized in that Step 1) The mass fraction of the methanol solution is 97-99.9%; the volume ratio of the 3-(trimethoxysilyl)propyl methacrylate to the methanol solution is 1:0.5-1.

5.

3. The use according to claim 1 or 2, It is characterized in that The temperature of the sealed storage in step 1) is 20 to 35° C., and the sealed storage time is 12 to 24 hours.

4. The use according to claim 3, It is characterized in that Step 2) The initiator is azobisisobutyronitrile or 2,2-dimethoxy-2-phenylacetophenone.

5. The use according to claim 4, It is characterized in that Step 2) The power of the ultrasonic mixing is 90-110 W, and the time of the ultrasonic mixing is 4-6 min.

6. The use according to claim 5, It is characterized in that The temperature of the in-situ thermal polymerization is 40-80° C., and the time of the in-situ thermal polymerization is 4-24 hours; the temperature of the photopolymerization is 18-26° C., and the time of the photopolymerization is 15-35 minutes.

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