A terpenoid biosynthetic gene cluster and its expression cassette, expression vector and recombinant microbial strain

Abstract

The present invention provides a terpenoid biosynthetic gene cluster, its expression cassette, expression vector, and recombinant microbial strain. The terpenoid biosynthetic gene cluster comprises at least one of a sesquiterpenoid and a diterpenoid. The terpenoid biosynthetic gene cluster comprises four gene clusters, and a plasmid library and a strain library containing functional genes from the gene clusters are obtained. The present invention ferments and tests the obtained strain library to obtain a product library including 36 terpenoid compounds. Furthermore, the present invention identifies the structures of four terpenoid compounds through product purification and NMR analysis. The terpenoid gene clusters screened by the present invention are capable of producing sesquiterpenoid and diterpenoid compounds, enriching the variety and library capacity of terpenoid natural products.

Description

Technical Field

[0001] The present invention relates to the field of bioengineering technology, and in particular to a terpenoid biosynthesis gene cluster and an expression box, an expression vector and a recombinant microbial strain thereof. Background Art

[0002] Terpenoids are widely found in plants, fungi, bacteria, and other eukaryotic organisms. Plants are the primary source of terpenoids, primarily monoterpenes and sesquiterpenes, such as limonene, artemisinin, and valerian. Smaller amounts of diterpenes, triterpenes, and tetraterpenes are also present. The rich variety and highly diverse structures of terpenoids give them diverse biological activities and widespread applications. Examples include the clinically used anti-tumor drug paclitaxel and the antimalarial drug artemisinin; antioxidants such as lycopene, β-carotene, and astaxanthin; ingenol, which exhibits anticancer and antiviral activities; and the antibacterial truncated pleuromutilin. In addition to terpenoids already developed as drugs, food additives, or nutritional supplements, researchers have discovered numerous other terpenoids with promising biological activities, including Mangicol A, which exhibits excellent anti-inflammatory activity and low cytotoxicity; Englerin A, which exhibits excellent anti-renal cancer activity; Salvicine, an anti-tumor substance with potential drug development; Lupeol, a substance with diverse biological activities including anti-inflammatory, anti-tumor, and cardiovascular therapeutics; Limonene, which has potential anti-tumor activity in addition to its use in the perfume and fragrance industry; and Linalool, an anti-inflammatory compound. Terpenoids are so diverse and diverse in their uses that they play a crucial role in our daily lives and have garnered widespread attention in the medical, food, and cosmetics industries, as well as in scientific research.

[0003] In addition to the terpenoid compounds that have been discovered, there are still a large number of unknown terpenoid compounds in nature waiting to be explored by researchers. Therefore, it is particularly important to seek efficient terpenoid research methods to help advance the progress of this research field. Summary of the Invention

[0004] The present invention aims to provide a terpenoid biosynthetic gene cluster and its expression cassette, expression vector and recombinant microbial strain. The research targets fungal terpenoid products and uses the recognized safe strain Aspergillus oryzae as a heterologous expression host. Based on an automated high-throughput platform, the terpenoid biosynthetic gene cluster is reconstructed and functionally characterized on a large scale. This improves the throughput of terpenoid natural product gene cluster research, enriches the types of terpenoid products, and accelerates the research progress in the field of natural product biosynthesis.

[0005] In a first aspect of the present invention, a gene cluster for the biosynthesis of a terpenoid is provided, wherein the terpenoid comprises at least one of a sesquiterpene, a diterpene, and a sesquiterpene;

[0006] The terpenoid biosynthesis gene cluster includes at least one of the following four gene clusters;

[0007] Gene cluster BGC1: contains Aat08891, Aat08894, Aat08897, and Aat08898 genes, encoding Monooxygenase, Reductase, TS, and P450 enzymes, respectively. Their nucleotide sequences are shown in SEQ ID NO. 5 to SEQ ID NO. 8, respectively.

[0008] Gene cluster BGC2: includes Aat09925, Aat09929, Aat09930, and Aat09932 genes, encoding dehydrogenase, P450, TS, and ketoruductase, respectively. Their nucleotide sequences are shown in SEQ ID NO. 9 to SEQ ID NO. 12, respectively.

[0009] Gene cluster BGC3: includes genes Cgl11904, Cgl11905, Cgl11909, and Cgl11915, encoding P450, Reductase, TS, and Ergosterol synthase, respectively. Their nucleotide sequences are shown in SEQ ID NO. 13 to SEQ ID NO. 16, respectively.

[0010] Gene cluster BGC4: includes Cgl13302 gene, Cgl13303 gene, Cgl13304 gene, Cgl13305 gene, Cgl13306 gene, Cgl13308 gene, Cgl13309 gene, Cgl13310 gene and Cgl13314 gene, which encode P450, Dioxygenase, P450, NRPS, Methyltransferase, Hydroxylase, TS, Short-chain dehydrogenase reductase and Dehydrogenase, respectively, and their nucleotide sequences are shown in SEQ ID NO.17 to SEQ ID NO.25, respectively.

[0011] Furthermore, the nucleotide sequences of gene clusters BGC1 to BGC4 among the four gene clusters are shown as SEQ ID NO.1 to SEQ ID NO.4, respectively.

[0012] In a second aspect of the present invention, an expression cassette of a terpenoid biosynthetic gene cluster is provided, wherein the expression cassette contains the terpenoid biosynthetic gene cluster.

[0013] Furthermore, the expression cassette further contains a promoter and a terminator, wherein the promoter includes one of PhlyA, PamyB and PglaA, and the terminator includes one of TagdA, TamyB and TniaD.

[0014] The nucleotide sequences of PhlyA, PamyB and PglaA are shown in SEQ ID NO. 26 to SEQ ID NO. 28, respectively; the nucleotide sequences of TagdA, TamyB and TniaD are shown in SEQ ID NO. 29 to SEQ ID NO. 31, respectively;

[0015] In the third aspect of the present invention, an expression vector of a terpenoid biosynthesis gene cluster is provided, wherein the expression vector contains the expression cassette.

[0016] In a fourth aspect of the present invention, a recombinant microbial strain of a terpenoid biosynthetic gene cluster is provided, wherein the recombinant microbial strain contains the expression vector.

[0017] Furthermore, the microorganism includes one of Aspergillus oryzae, Escherichia coli, Saccharomyces cerevisiae, Pichia pastoris, Bacillus, Aspergillus nidulans, Aspergillus niger, Neurospora crassa, Alternaria alternata or Fusarium.

[0018] In a fifth aspect of the present invention, a method for biosynthesizing terpenoids is provided, comprising:

[0019] Constructing microbial mutants that heterologously synthesize sesquiterpenoid or diterpenoid biosynthetic gene clusters;

[0020] fermenting the microbial mutant to obtain a fermentation culture;

[0021] The fermentation culture is extracted, separated and the product is purified to obtain terpenoid compounds.

[0022] Furthermore, the microorganism is any one of Aspergillus oryzae, Escherichia coli, Saccharomyces cerevisiae, Pichia pastoris, Bacillus, Aspergillus oryzae, Aspergillus nidulans, Aspergillus niger, Neurospora crassa, Alternaria alternata or Fusarium.

[0023] One or more technical solutions in the embodiments of the present invention have at least the following technical effects or advantages:

[0024] The present invention provides a terpenoid biosynthetic gene cluster and its expression cassette, expression vector, and recombinant microbial strain. With fungal terpenoid products as the research target and the recognized safe strain Aspergillus oryzae as the heterologous expression host, the present invention uses an automated high-throughput platform to conduct large-scale heterologous reconstruction and functional characterization of the terpenoid biosynthetic gene cluster. This improves the throughput of terpenoid natural product gene cluster research, enriches the variety of terpenoid products, and accelerates the research progress in the field of natural product biosynthesis. Specifically:

[0025] (1) In the present invention, Aspergillus oryzae is preferably used as the dominant expression host. Through rational design and based on the established automated high-throughput platform, four terpenoid natural product biosynthetic gene clusters were efficiently heterologously reconstructed in it, and a strain library containing 29 strains was obtained;

[0026] (2) The present invention obtains a terpenoid compound library containing 36 products through high-throughput fermentation culture and detection of the strain library;

[0027] (3) The present invention obtained four terpenoid compound structures by expanding the strain library, isolating, purifying and structurally identifying the products. BRIEF DESCRIPTION OF THE DRAWINGS

[0028] In order to more clearly illustrate the technical solutions in the embodiments of the present invention, the following is a brief introduction to the drawings required for use in the description of the embodiments. Obviously, the drawings described below are some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying any creative work.

[0029] Figure 1 A schematic diagram summarizing a terpenoid biosynthesis gene cluster screening library according to one embodiment of the present invention;

[0030] Figure 2 Schematic diagram of bioinformatics analysis, strain construction, and product detection of the gene cluster BGC1-AaT08897 of the present invention; wherein: Figure 2 a is the bioinformatics analysis results of gene cluster BGC1-AaT08897; Figure 2 b is a schematic diagram of heterologous reconstruction of the BGC1-AaT08897 gene cluster; Figure 2 c is the result of strain GC / MS detection; Figure 2 d is the result of HR-LC / MS detection of the strain;

[0031] Figure 3 GC / MS and HR-LC / MS detection spectra of gene cluster BGC1-AaT08897;

[0032] Figure 4Schematic diagram of bioinformatics analysis, strain construction, and product detection of the gene cluster BGC2-AaT09930 of the present invention; wherein: Figure 4 a is the bioinformatics analysis results of gene cluster BGC2-AaT09930; Figure 4 b is a schematic diagram of heterologous reconstruction of the BGC2-AaT09930 gene cluster; Figure 4 c is the result of strain GC / MS detection; Figure 4 d is the result of HR-LC / MS detection of the strain; Figure 4 e is the GC / MS and HR-LC / MS detection spectrum;

[0033] Figure 5 Schematic diagram of bioinformatics analysis, strain construction, and product detection of the gene cluster BGC3-Cgl11909 of the present invention; wherein: Figure 5 a is the bioinformatics analysis results of gene cluster BGC15-Cgl11909; Figure 5 b is a schematic diagram of heterologous reconstruction of the BGC15-Cgl11909 gene cluster; Figure 5 c is the result of strain GC / MS detection; Figure 5 d is the GC / MS detection spectrum;

[0034] Figure 6 Schematic diagram of bioinformatics analysis and strain construction of gene cluster BGC4-Cgl3309 of the present invention; wherein: Figure 6 a is the bioinformatics analysis results of gene cluster BGC17-Cgl3309; Figure 6 b is a schematic diagram of heterologous reconstruction of the BGC17-Cgl3309 gene cluster;

[0035] Figure 7 GC / MS and HR-LC / MS detection spectra of gene cluster BGC4-Cgl3309; wherein: Figure 7 a is the result of strain GC / MS detection; Figure 7 b is the result of HR-LC / MS detection of strains; Figure 7 c is the result of strain GC / MS detection;

[0036] Figure 8 This is a schematic structural diagram of compound (6) according to one embodiment of the present invention;

[0037] Figure 9 is the 1HNMR spectrum of compound (6) of the present invention;

[0038] Figure 10 is the 13C NMR spectrum of compound (6) of the present invention;

[0039] Figure 111H-1H COSY spectrum of compound (6) of the present invention;

[0040] Figure 12 is the HSQC spectrum of compound (6) of the present invention;

[0041] Figure 13 is the HMBC spectrum of compound (6) of the present invention;

[0042] Figure 14 This is the ROESY spectrum of compound (6) of the present invention.

[0043] Figure 15 Schematic diagram of the structure of compound (35) according to one embodiment of the present invention;

[0044] Figure 16 is the 1HNMR spectrum of compound (35) of the present invention;

[0045] Figure 17 is the 13C NMR spectrum of compound (35) of the present invention;

[0046] Figure 18 1H-1H COSY spectrum of compound (35) of the present invention;

[0047] Figure 19 is the HSQC spectrum of compound (35) of the present invention;

[0048] Figure 20 is the HMBC spectrum of compound (35) of the present invention;

[0049] Figure 21 This is the ROESY spectrum of compound (35) of the present invention.

[0050] Figure 22 Schematic diagram of the structure of compound (136) according to one embodiment of the present invention;

[0051] Figure 23 is the 1HNMR spectrum of compound (136) of the present invention;

[0052] Figure 24 13C NMR spectrum of compound (136) of the present invention;

[0053] Figure 25 1H-1H COSY spectrum of compound (136) of the present invention;

[0054] Figure 26 is the HSQC spectrum of compound (136) of the present invention;

[0055] Figure 27 is the HMBC spectrum of compound (136) of the present invention;

[0056] Figure 28 This is the ROESY spectrum of the compound (136) of the present invention.

[0057] Figure 29 Schematic diagram of the structure of compound (144) according to one embodiment of the present invention;

[0058] Figure 30 is the 1HNMR spectrum of compound (144) of the present invention;

[0059] Figure 31 13C NMR spectrum of compound (144) of the present invention;

[0060] Figure 32 1H-1H COSY spectrum of compound (144) of the present invention;

[0061] Figure 33 is the HSQC spectrum of compound (144) of the present invention;

[0062] Figure 34 is the HMBC spectrum of compound (144) of the present invention;

[0063] Figure 35 This is the ROESY spectrum of the compound (144) of the present invention. DETAILED DESCRIPTION

[0064] The present invention will be described in detail below in conjunction with specific embodiments and examples, and the advantages and various effects of the present invention will be more clearly presented. It should be understood by those skilled in the art that these specific embodiments and examples are for illustrating the present invention, rather than for limiting the present invention.

[0065] Throughout this specification, unless otherwise specified, the terms used herein should be understood as having the same meaning as commonly used in the art. Therefore, unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. In the event of any conflict, the present specification shall take precedence.

[0066] Unless otherwise specified, various raw materials, reagents, instruments and equipment used in the present invention can be purchased from the market or obtained through existing methods.

[0067] The technical solution of the embodiment of the present application is to solve the above technical problems, and the overall idea is as follows:

[0068] In the first part, the present invention finally locked in four gene clusters that can synthesize sesquiterpenes, diterpenes or disesquiterpenes compounds through bioinformatics analysis.

[0069] In the second part, the present invention amplifies the four gene clusters, promoter, terminator, and linear vector fragments and adopts a yeast assembly method to transfer them into cerevisiae using an automated pipetting workstation method, and then extracts the plasmid to obtain a plasmid library.

[0070] In the third part, the present invention rationally combines the plasmid library obtained above according to the different functional elements (terpenoid synthase, cytochrome P450 enzyme, acyltransferase, glycosyltransferase) contained, and transforms them into microbial strains based on an automated platform to obtain a microbial strain library. The microorganism is not limited to Aspergillus oryzae, but includes any one of Aspergillus oryzae, Escherichia coli, Saccharomyces cerevisiae, Pichia pastoris, Bacillus, Aspergillus nidulans, Aspergillus niger, Neurospora crassa, Alternaria alternata or Fusarium.

[0071] In the fourth part, the present invention ferments and detects the microbial strain library obtained above, and obtains a terpenoid compound library based on comparison analysis with the NIST database and high-resolution mass spectrometry fragmentation inference analysis.

[0072] In the fifth part, the present invention expands the culture and fermentation of the microbial strain corresponding to the terpenoid compounds detected and obtained in the fourth part, separates and purifies the products, and analyzes the structure of the compounds by nuclear magnetic resonance spectroscopy.

[0073] The following is a detailed description of the biosynthetic gene cluster of a terpenoid compound of the present application, its expression cassette, expression vector and recombinant microbial strain in combination with the examples and experimental data.

[0074] Example 1. Bioinformatics analysis of terpenoid biosynthesis gene clusters

[0075] Based on AntiSMASH software, bioinformatics analysis of terpenoid biosynthesis gene clusters in two filamentous fungi from different sources, Alternaria alternata TPF6 and Colletotrichum gloeosporioides ES026, was performed, and terpenoid biosynthesis gene clusters were found in both strains. By further analyzing the conserved domains of the first step catalytic enzyme terpenoid synthase (type I terpenoid synthase DDXXD / E, type II terpenoid synthase DXDD) in the predicted gene clusters, gene clusters without conserved domains or incomplete functional domains were removed, and finally four gene clusters capable of synthesizing sesquiterpenes, diterpenes or disesquiterpenes were identified. The nucleotide sequences of the four gene clusters are shown in SEQ ID NO.1-SEQ ID NO.4 respectively; each gene cluster was identified to contain several functional genes, as shown in Table 1 ( Figure 1 ).

[0076] Table 1

[0077]

[0078] Example 2: Construction of overexpression vector

[0079] 1. High-throughput amplification of recombinant fragments

[0080] The DNA fragments required for constructing the recombinant plasmid library were obtained by PCR amplification. Phusion high-fidelity DNA polymerase was purchased from New England Biolabs (NEB), and Prime STAR GXL DNA polymerase was purchased from TaKaRa Bio, Inc. (TaKaRa Bio, Inc., Shiga, Japan). PCR primers were purchased from GenScript Biotech Co., Ltd. Magnetic beads used for PCR purification of the amplified fragments were purchased from Vazyme (N411-01).

[0081] All functional genes were amplified from the genomes of two filamentous fungi, Alternaria alternata TPF6 and Colletotrichum gloeosporioides ES026. The promoters (PhlyA, PamyB, and PglaA) and terminators (TagdA, TamyB, and TniaD) used were amplified from plasmids pSC19 (containing PhlyA), pTAex3-optimized (containing PamyB and TamyB), pGB98 (containing PglaA), pYJ177 (containing TagdA), and pGB127 (containing TniaD), respectively. The vector fragments were amplified from plasmids pTAex3-optimized, pUSA-optimized, and pAdeA-optimized, respectively. The plasmid template information required for amplification is shown in Table 2.

[0082] Among them, the nucleotide sequence of the pTAex3-optimized vector is shown in SEQ ID NO.32; the nucleotide sequence of the pUSA-optimized vector is shown in SEQ ID NO.33; the nucleotide sequence of the pAdeA-optimized vector is shown in SEQ ID NO.34; the pGB98 vector reference is doi: https: / / doi.org / 10.1002 / biot.201600697; the pYJ177 vector refers to the patent CN202010936790.X previously applied for by the inventor of this application; other vectors are all vectors disclosed in the prior art.

[0083] The 5'-end and 3'-end of all functional genes, promoters, terminators and vector sequences contain overlapping fragments of 40bp bases, so that the plasmid library can be assembled using the inherent recombination ability of Saccharomyces cerevisiae in the future. The preparation of the PCR system is completed by an automated pipetting workstation (Biomek FXP Laboratory Automation Workstation, Beckman Coulter). PCR amplification adopts a 40 μL amplification system, and the required primers are synthesized and dissolved and then sub-packed in 96-well plates. After the DNA polymerase system (containing buffer, dNTPs, DNA polymerase) is prepared in the required amount, it is evenly divided into 96-well plates (34 μL / well), and the template is diluted and sub-packed in 96-well plates (25ng / μL). 2 μL of front primers, 2 μL of rear primers and 2 μL of templates are taken in sequence and added to the DNA polymerase system. After mixing by pipetting, they are transferred to a PCR instrument and the program is set to perform PCR amplification. The amplification program used in this embodiment is as follows:

[0084]

[0085] 2. Yeast assembly and construction of recombinant plasmid library

[0086] Recombinant plasmids were primarily constructed using yeast assembly. Saccharomyces cerevisiae CEN.PK2-1D, EUROSCARF (genotype MATaura3-52trp1-289leu2-3112his3Δ1MAL2-8C SUC2) was used as the experimental strain for homologous recombination, and recombinant plasmids were constructed using an optimized lithium acetate / polyethylene glycol (LiAc / PEG) chemical transformation method.

[0087] 300 ng of each coding sequence, promoter, terminator, and linearized vector fragment required for constructing each recombinant plasmid were mixed (total volume 10 μL). Using the MP20096-Tip robotic arm in an automated pipetting workstation, 10 μL of the DNA mixture was transferred to a 96-well plate containing Saccharomyces cerevisiae competent cells (100 μL / well). Boiled fish sperm DNA (20 μL / well) and a LiAc / TE / 40% PEG4000 solution (700 μL / well) were then added sequentially. The plate was set to the "aspirate" setting, pipetted to mix thoroughly, and incubated in a 30°C incubator for 30 min. The plate was removed, DMSO (88 μL / well) was added, pipetted to mix thoroughly, and the plate was transferred to a 42°C incubator for a heat shock of 8 min. The plate was centrifuged at 1500 rpm for 5 min, the supernatant discarded, and the cells resuspended in 1 mL of YPD medium. The plate was then transferred to a 30°C incubator and incubated for 60 min. Remove the culture plate and centrifuge at 1500 rpm for 5 minutes. Discard the supernatant and wash the cells with 1 mL of TE solution. Centrifuge at 1500 rpm for 5 minutes, discard some of the supernatant, and resuspend the cells in approximately 100 μL of TE solution. Pipette the bacterial solution into a 96-well deep-well plate containing 1 mL of uracil-deficient solid culture medium (SC-Ura). Transfer the culture plate to a shaking module and set the shake program to 30 seconds to evenly spread the bacterial solution on the solid culture medium. Transfer the culture plate to a 30°C incubator and incubate for 3 days. Once the cells grow, transfer them to SC-Ura liquid culture medium for culture and extract the yeast plasmid.

[0088] Specifically, the overexpression vector constructed in the embodiment of the present invention is as follows:

[0089] The following plasmids were constructed using the yeast assembly method, using the yeast host CEN.PK2-1D. The TPF6 genome and ES026 genome represent the genomes of Alternaria alternate TPF6 and Colletotrichum gloeosporioides ES026, respectively.

[0090] Construction of pYJ234 plasmid: Using pYJ153, pYJ177, TPF6 genome, and pYJ177 as templates, primer pairs P81 / P82, P17 / P83, P18 / P19, and P84 / P20 were used to amplify fragments pYJ234-F1, pYJ234-F2, pYJ234-F3, and pYJ234-F4, respectively. The above four fragments were assembled in yeast to obtain plasmid pYJ234.

[0091] Construction of pYJ235 plasmid: Using pYJ177, pYJ177, TPF6 genome, and pYJ177 as templates, primer pairs P81 / P85, P21 / P86, P22 / P23, and P84 / P24 were used to amplify fragments pYJ235-F1, pYJ235-F2, pYJ235-F3, and pYJ235-F4, respectively. The above four fragments were assembled in yeast to obtain plasmid pYJ235.

[0092] Construction of pYJ236 plasmid: Using pYJ153, pYJ177, TPF6 genome, pYJ177, pYJ177, TPF6 genome, and pYJ153 as templates, primer pairs P87 / P82, P17 / P83, P18 / P19, P88 / P20, P89 / P25, P26 / P27, and P28 / P90 were used to amplify fragments pYJ236-F1, pYJ236-F2, pYJ236-F3, pYJ236-F4, pYJ236-F5, pYJ236-F6, and pYJ236-F7, respectively. The above 7 fragments were assembled in yeast to obtain plasmid pYJ236.

[0093] Construction of pYJ237 plasmid: Using pYJ153, pYJ177, TPF6 genome, and pYJ177 as templates, primer pairs P81 / P82, P91 / P83, P99P92 / P93, and P84 / P94 were used to amplify fragments pYJ237-F1, pYJ237-F2, pYJ237-F3, and pYJ237-F4, respectively. The above four fragments were assembled in yeast to obtain plasmid pYJ237.

[0094] Construction of pYJ238 plasmid: Using pYJ177, pYJ177, TPF6 genome, and pYJ177 as templates, primer pairs P81 / P85, P95 / P86, P96 / P97, and P84 / P98 were used to amplify fragments pYJ238-F1, pYJ238-F2, pYJ238-F3, and pYJ238-F4, respectively. The above four fragments were assembled in yeast to obtain plasmid pYJ238.

[0095] Construction of pYJ239 plasmid: Using pYJ153, pYJ177, TPF6 genome, pYJ177, pYJ177, TPF6 genome, and pYJ153 as templates, primer pairs P87 / P82, P91 / P83, P99P92 / P93, P104 / P94, P89 / P100, P101 / P102, and P103 / P90 were used to amplify fragments pYJ239-F1, pYJ239-F2, pYJ239-F3, pYJ239-F4, pYJ239-F5, pYJ239-F6, and pYJ239-F7, respectively. The above 7 fragments were assembled in yeast to obtain plasmid pYJ239.

[0096] Construction of pYJ288 plasmid: Using pYJ153, pYJ177, ES026 genome, and pYJ177 as templates, primer pairs P81 / P82, P29 / P83, P30 / P31, and P84 / P32 were used to amplify fragments pYJ288-F1, pYJ288-F2, pYJ288-F3, and pYJ288-F4, respectively. The above four fragments were assembled in yeast to obtain plasmid pYJ288.

[0097] Construction of pYJ289 plasmid: Using pYJ177, pYJ177, ES026 genome, and pYJ177 as templates, primer pairs P81 / P85, P33 / P86, P34 / P35, and P84 / P36 were used to amplify fragments pYJ289-F1, pYJ289-F2, pYJ289-F3, and pYJ289-F4, respectively. The above four fragments were assembled in yeast to obtain plasmid pYJ289.

[0098] Construction of pYJ290 plasmid: Using pYJ153, pYJ177, ES026 genome, pYJ177, pYJ177, ES026 genome, and pYJ153 as templates, primer pairs P87 / P82, P29 / P83, P30 / P31, P88 / P32, P89 / P37, P38 / P39, and P40 / P90 were used to amplify fragments pYJ290-F1, pYJ290-F2, pYJ290-F3, pYJ290-F4, pYJ290-F5, pYJ290-F6, and pYJ290-F7, respectively. The above 7 fragments were assembled in yeast to obtain plasmid pYJ290.

[0099] Construction of pYJ295 plasmid: Using pYJ153, pYJ177, ES026 genome, and pYJ177 as templates, primer pairs P81 / P82, P41 / P83, P42 / P43, and P84 / P44 were used to amplify fragments pYJ295-F1, pYJ295-F2, pYJ295-F3, and pYJ295-F4, respectively. The above four fragments were assembled in yeast to obtain plasmid pYJ295.

[0100] Construction of pYJ296 plasmid: Using pYJ177, pYJ177, ES026 genome, and pYJ177 as templates, primer pairs P81 / P85, P45 / P86, P46 / P47, and P84 / P48 were used to amplify fragments pYJ296-F1, pYJ296-F2, pYJ296-F3, and pYJ296-F4, respectively. The above four fragments were assembled in yeast to obtain plasmid pYJ296.

[0101] Construction of pYJ297 plasmid: Using pYJ153, pYJ177, ES026 genome, pYJ177, pYJ177, ES026 genome, and pYJ153 as templates, primer pairs P87 / P82, P41 / P83, P42 / P43, P88 / P44, P89 / P49, P50 / P51, and P52 / P90 were used to amplify fragments pYJ297-F1, pYJ297-F2, pYJ297-F3, pYJ297-F4, pYJ297-F5, pYJ297-F6, and pYJ297-F7, respectively. The above 7 fragments were assembled in yeast to obtain plasmid pYJ297.

[0102] pYJ298 plasmid construction: pYJ153, pYJ177, ES026 genome, pYJ177, pYJ177, ES026 genome, pYJ153, pYJ153, ES026 The genome was used as a template, and primer pairs P56 / P82, P41 / P83, P42 / P43, P104 / P44, P89 / P49, P105 / P51, P52 / P106, P53 / P107, and P54 / P55 were used to amplify fragments pYJ298-F1, pYJ298-F2, pYJ298-F3, pYJ298-F4, pYJ298-F5, pYJ298-F6, pYJ298-F7, pYJ298-F8, and pYJ298-F9, respectively. The above 9 fragments were assembled in yeast to obtain plasmid pYJ298.

[0103] Construction of pYJ299 plasmid: pYJ177, pYJ177, ES026 genome, pYJ177, pYJ177, ES026 genome, and pYJ153 were used as templates, and primer pairs P87 / P85, P45 / P86, P46 / P47, P88 / P48, PglaA-PhlyA GF(177) / P57, P58 / P59, and P60 / P90 were used to amplify fragments pYJ299-F1, pYJ299-F2, pYJ299-F3, pYJ299-F4, pYJ299-F5, pYJ299-F6, and pYJ299-F7, respectively. The above 7 fragments were assembled in yeast to obtain plasmid pYJ299.

[0104] pYJ300 plasmid construction: pYJ177, pYJ177, ES026 genome, pYJ177, pYJ177, ES026 genome, pYJ153, pYJ153, ES026 The genome was used as a template, and primer pairs P64 / P85, P45 / P86, P46 / P47, P104 / P48, P89 / P57, P58 / P59, P60 / P106, P61 / P107, and P62 / P63 were used to amplify fragments pYJ300-F1, pYJ300-F2, pYJ300-F3, pYJ300-F4, pYJ300-F5, pYJ300-F6, pYJ300-F7, pYJ300-F8, and pYJ300-F9, respectively. The above 9 fragments were assembled in yeast to obtain plasmid pYJ300.

[0105] Construction of pYJ301 plasmid: Using pGB394, pYJ177, ES026 genome, and pYJ177 as templates, primer pairs P65 / P72, P66 / P67, P68 / P69, and P70 / P71 were used to amplify fragments pYJ301-F1, pYJ301-F2, pYJ301-F3, and pYJ301-F4, respectively. The above four fragments were assembled in yeast to obtain plasmid pYJ301.

[0106] Construction of pYJ302 plasmid: Using pGB394, pYJ177, ES026 genome, pYJ177, pYJ177, ES026 genome, and pYJ153 as templates, primer pairs P65 / P80, P66 / P67, P68 / P69, P70 / P73, P75 / P74, Cgl13314-Pamy GF(302) / P77, and P78 / P79 were used to amplify fragments pYJ302-F1, pYJ302-F2, pYJ302-F3, pYJ302-F4, pYJ302-F5, pYJ302-F6, and pYJ302-F7, respectively. The above 7 fragments were assembled in yeast to obtain plasmid pYJ302.

[0107] Construction of pGB363 plasmid: Using TPF6 genome and pYJ152 as templates, primer pairs P2 / P1 and P4 / P3 were used to amplify fragments pGB363-F1 and pGB363-F2, respectively. The above two fragments were assembled in yeast to obtain plasmid pGB363.

[0108] Construction of pGB364 plasmid: Using TPF6 genome and pYJ152 as templates, primer pairs P6 / P5 and P8 / P7 were used to amplify fragments pGB364-F1 and pGB364-F2, respectively. The above two fragments were assembled in yeast to obtain plasmid pGB364.

[0109] Construction of pGB392 plasmid: Using the ES026 genome and pYJ152 as templates, primer pairs P9 / P10 and P11 / P12 were used to amplify fragments pGB392-F1 and pGB392-F2, respectively. The above two fragments were assembled in yeast to obtain plasmid pGB392.

[0110] Construction of pGB394 plasmid: Using the ES026 genome and pYJ152 as templates, primer pairs P13 / P14 and P15 / P16 were used to amplify fragments pGB394-F1 and pGB394-F2, respectively. The above two fragments were assembled in yeast to obtain plasmid pGB394.

[0111] Table 2 - Plasmids constructed in the present invention

[0112]

[0113] Table 3 - Primers used to construct plasmids of the present invention

[0114]

[0115]

[0116]

[0117] 3. Enrichment of recombinant plasmids in E. coli

[0118] Escherichia coli DH10B competent cells were prepared using the CaCl2 method. Competent cells were dispensed into 96-well deep-well plates at 70 μL / well using an automated dispenser. 10 μL of plasmid DNA solution was added to the E. coli DH10B competent cells and incubated at 4°C for 30 minutes. The plate containing the cells was then transferred to a preheated 42°C incubator and incubated for 3 minutes. Afterward, 800 μL of LB medium was added to each well and incubated at 37°C for 45 minutes to allow the cells to recover. The plate was then transferred to a centrifuge and centrifuged at 1500 g for 8 minutes to harvest the cells. The supernatant was discarded, and the remaining 50 μL of culture medium was used to resuspend the cells. The cells were then plated onto LA solid medium containing ampicillin (Amp) and cultured overnight at 37°C. Using a Qpix 460 instrument, the cells were transferred to LB medium and expanded to enrich the plasmid. The obtained plasmid was verified by restriction enzyme digestion.

[0119] Example 3: Construction of Aspergillus oryzae mutants capable of synthesizing terpenoids

[0120] Through bioinformatics analysis, the terpenoid biosynthesis gene cluster can be divided into three synthesis modules:

[0121] (1) Upstream terpene synthase module: contains terpene synthase (TS), which catalyzes the synthesis of terpene skeleton compounds from precursors (such as FPP, GGPP, and GFPP);

[0122] (2) Midstream oxidation module: contains cytochrome protein P450 (CYP450) enzymes, which catalyze the synthesis of double bonds, carbonyl or hydroxyl functional groups from the core skeleton;

[0123] (3) Downstream post-modification module: Contains optional post-modification catalytic enzymes, such as acyltransferase (ACT) and glycosylation enzyme (GE), which catalyze the intermediate compounds synthesized in (2) above, thereby synthesizing structurally diverse and potentially active terpenoid compounds.

[0124] Therefore, to achieve high-throughput reconstruction of terpenoid gene clusters, obtain intermediates and final terpenoid compounds, and elucidate their biosynthetic pathways, the present invention utilizes the inherent sequential catalytic properties of terpenoid gene cluster synthesis to rationally design combinatorial transformation recombinant plasmids and construct a screening library for Aspergillus oryzae mutants. The Aspergillus oryzae strain used in the present invention is specifically Aspergillus oryzae NSAR1, and the plasmids pUSA, pAdeA, and pTAex3 used were all donated by Professor Ikuro Abe of the University of Tokyo, Japan.

[0125] In the strain construction, the protoplast transformation method was used. Plasmids containing different genes were transformed into Aspergillus oryzae based on an automated pipetting workstation. As shown in Table 4, the operation steps are as follows:

[0126] 1. Construction of recombinant Aspergillus oryzae strains related to BGC1-AaT08897

[0127] 1. AO-Y11: obtained by co-transformation of plasmid pGB363 / pUSA / pAdeA into Aspergillus oryzae, containing terpene synthase AaT08897;

[0128] 2. AO-Y12: obtained by co-transformation of plasmid pGB363 / pYJ234 / pAdeA into Aspergillus oryzae, containing terpenoid synthase AaT08897 and cytochrome P450 enzyme AaT08898;

[0129] 3. AO-Y13: obtained by co-transformation of plasmid pGB363 / pUSA / pYJ235 into Aspergillus oryzae, containing terpenoid synthase AaT08897 and monooxygenase AaT08891;

[0130] 4. AO-Y14: obtained by co-transformation of plasmid pGB363 / pYJ234 / pYJ235 into Aspergillus oryzae, containing terpenoid synthase AaT08897, cytochrome P450 enzyme AaT08898, and monooxygenase AaT08891;

[0131] 5. AO-Y15: obtained by co-transformation of plasmid pGB363 / pYJ236 / pAdeA into Aspergillus oryzae, containing terpenoid synthase AaT08897, cytochrome P450 enzyme AaT08898, and reductase AaT08894;

[0132] 6. AO-Y16: obtained by co-transformation of plasmid pGB363 / pYJ236 / pYJ235 into Aspergillus oryzae, containing terpenoid synthase AaT08897, cytochrome P450 enzyme AaT08898, reductase AaT08894 and monooxygenase AaT08891.

[0133] 2. Recombinant Aspergillus oryzae strains related to BGC2-AaT09930

[0134] 1. AO-Y17: obtained by co-transformation of plasmid pGB364 / pUSA / pAdeA into Aspergillus oryzae, containing terpene synthase AaT09930;

[0135] 2. AO-Y18: obtained by co-transformation of plasmid pGB364 / pYJ237 / pAdeA into Aspergillus oryzae, containing terpenoid synthase AaT09930 and cytochrome P450 enzyme AaT09929;

[0136] 3. AO-Y19: obtained by co-transformation of plasmid pGB364 / pYJ237 / pYJ238 into Aspergillus oryzae, containing terpenoid synthase AaT09930, cytochrome P450 enzyme AaT09929, and ketoreductase AaT09932;

[0137] 4. AO-Y20: obtained by co-transformation of plasmid pGB364 / pYJ239 / pYJ238 into Aspergillus oryzae, containing terpenoid synthase AaT09930, cytochrome P450 enzyme AaT09929, dehydrogenase AaT09925 and ketoreductase AaT09932.

[0138] 3. Recombinant Aspergillus oryzae strains related to BGC3-Cgl11909

[0139] 1. AO-Y121: obtained by co-transformation of plasmid pGB392 / pUSA / pAdeA into Aspergillus oryzae, containing terpene synthase Cgl11909;

[0140] 2. AO-Y122: obtained by co-transformation of plasmid pGB392 / pYJ288 / pAdeA into Aspergillus oryzae, containing terpenoid synthase Cgl11909 and cytochrome P450 enzyme Cgl11904;

[0141] 3. AO-Y123: obtained by co-transformation of plasmid pGB392 / pYJ288 / pYJ289 into Aspergillus oryzae, containing terpene synthase Cgl11909, cytochrome P450 enzyme Cgl11904, and reductase Cgl11905;

[0142] 4. AO-Y124: obtained by co-transforming plasmid pGB392 / pYJ290 / pYJ289 into Aspergillus oryzae, containing terpene synthase Cgl11909, cytochrome P450 enzyme Cgl11904, ergosterol synthase Cgl11915 and reductase Cgl11905.

[0143] IV. Recombinant Aspergillus oryzae strains related to BGC4-Cgl13309

[0144] 1. AO-Y131: obtained by co-transformation of plasmid pGB394 / pUSA / pAdeA into Aspergillus oryzae, containing terpene synthase Cgl13309;

[0145] 2. AO-Y132: obtained by co-transformation of plasmid pGB394 / pYJ295 / pAdeA into Aspergillus oryzae, containing terpenoid synthase Cgl13309 and cytochrome P450 enzyme Cgl13302;

[0146] 3. AO-Y133: obtained by co-transformation of plasmid pGB394 / pUSA / pYJ296 into Aspergillus oryzae, containing terpenoid synthase Cgl13309 and cytochrome P450 enzyme Cgl13304;

[0147] 4. AO-Y134: obtained by co-transformation of plasmid pGB394 / pYJ295 / pYJ296 into Aspergillus oryzae, containing terpenoid synthase Cgl13309, cytochrome P450 enzyme Cgl13302 and cytochrome P450 enzyme Cgl13304;

[0148] 5. AO-Y135: obtained by co-transformation of plasmid pGB394 / pYJ297 / pAdeA into Aspergillus oryzae, containing terpene synthase Cgl13309, cytochrome P450 enzyme Cgl13302, and hydroxylase Cgl13308;

[0149] 6. AO-Y136: obtained by co-transformation of plasmid pGB394 / pYJ298 / pAdeA into Aspergillus oryzae, containing terpenoid synthase Cgl13309, cytochrome P450 enzyme Cgl13302, hydroxylase Cgl13308, and dioxygenase Cgl13303;

[0150] 7. AO-Y137: obtained by co-transformation of plasmid pGB394 / pYJ297 / pYJ296 into Aspergillus oryzae, containing terpenoid synthase Cgl13309, cytochrome P450 enzyme Cgl13302, hydroxylase Cgl13308, and cytochrome P450 enzyme Cgl13304;

[0151] 8. AO-Y138: obtained by co-transformation of plasmid pGB394 / pYJ298 / pYJ296 into Aspergillus oryzae, containing terpenoid synthase Cgl13309, cytochrome P450 enzyme Cgl13302, hydroxylase Cgl13308, dioxygenase Cgl13303 and cytochrome P450 enzyme Cgl13304;

[0152] 9. AO-Y139: obtained by co-transforming plasmid pArg / pUSA / pYJ299 into Aspergillus oryzae, containing terpenoid synthase Cgl13309 and non-ribosomal peptide synthase Cgl13305;

[0153] 10. AO-Y140: obtained by co-transformation of plasmid pGB394 / pYJ298 / pYJ299 into Aspergillus oryzae, containing terpene synthase Cgl13309, cytochrome P450 enzyme Cgl13302, hydroxylase Cgl13308, dioxygenase Cgl13303, cytochrome P450 enzyme Cgl13304 and non-ribosomal peptide synthase Cgl13305;

[0154] 11. AO-Y141: obtained by co-transformation of plasmid pGB394 / pYJ298 / pYJ300 into Aspergillus oryzae, containing terpenoid synthase Cgl13309, cytochrome P450 enzyme Cgl13302, hydroxylase Cgl13308, dioxygenase Cgl13303, cytochrome P450 enzyme Cgl13304, nonribosomal peptide synthase Cgl13305, and methyltransferase Cgl13306;

[0155] 12. AO-Y142: obtained by co-transforming plasmid pYJ301 / pYJ298 / pYJ300 into Aspergillus oryzae, containing terpenoid synthase Cgl13309, short-chain dehydrogenase reductase Cgl13310, cytochrome P450 enzyme Cgl13302, hydroxylase Cgl13308, dioxygenase Cgl13303, cytochrome P450 enzyme Cgl13304, nonribosomal peptide synthase Cgl13305, and methyltransferase Cgl13306;

[0156] 13. AO-Y143: obtained by co-transformation of plasmid pYJ302 / pYJ298 / pYJ300 into Aspergillus oryzae, containing terpenoid synthase Cgl13309, short-chain dehydrogenase reductase Cgl13310, dehydrogenase Cgl13314, cytochrome P450 enzyme Cgl13302, hydroxylase Cgl13308, dioxygenase Cgl13303, cytochrome P450 enzyme Cgl13304, nonribosomal peptide synthase Cgl13305 and methyltransferase Cgl13306;

[0157] 14. AO-Y144: co-transformed plasmid pYJ301 / pUSA / pAdeA into Aspergillus oryzae, containing terpene synthase Cgl13309 and short-chain dehydrogenase reductase Cgl13310; AO-Y145 co-transformed plasmid pYJ302 / pUSA / pAdeA, containing terpene synthase Cgl13309, short-chain dehydrogenase reductase Cgl13310 and dehydrogenase Cgl13314.

[0158] Table 4 - Aspergillus oryzae mutants for synthesizing terpenoids constructed according to the present invention

[0159]

[0160]

[0161] Example 4 Synthesis and Detection of Terpenoids

[0162] The mutant strains in Example 3 were cultured according to the following culture conditions, specifically:

[0163] The strain constructed in Example 3 was transferred to a 24-well deep-well plate containing rice solid culture medium for culture. Each strain was transferred to 6 replicate wells (each well containing 1g of rice and 1.5mL of deionized water) at 30°C for 2 weeks of fermentation. Acetone (5mL / well) was added to the cultured 24-well plate and soaked at room temperature for 2h. The supernatant was transferred to a new 24-well deep-well plate, and ethyl acetate (5mL / well) was added to the bacteria and soaked at room temperature for 2h. The acetone and ethyl acetate extraction phases were combined, and the 24-well plate was transferred to a fume hood and evaporated naturally to obtain a crude extract. Through GC / MS and HR-LC / MS detection and analysis, the present invention detected 36 terpenoid compounds, and their corresponding GC / MS and HR-LC / MS spectra are shown as follows: Figures 2 to 7 As shown ( Figures 2 to 7 ).

[0164] Table 5

[0165]

[0166]

[0167] As can be seen from the above table, among the four gene clusters involved in the present invention, each gene cluster identified a terpene product to determine the product type of the gene cluster, that is, BGC1-AaT08897, BGC2-AaT09930, BGC3-Cgl11909 and BGC4-Cgl13309 are gene clusters responsible for the synthesis of sesquiterpenes, diterpenes, sesquiterpenes and sesquiterpenes products, respectively. The terpene products of the related mutant strains constructed by the genes of the corresponding gene clusters also correspond to the types of terpene products of the gene clusters.

[0168] Example 5: Purification and structural identification of terpenoids

[0169] The verified strain was transferred to a plastic lunch box containing rice solid culture medium (each box contained 120g of rice and 100mL of deionized water) for the expansion of the bacterial cell culture to extract and purify the target compound. After the bacterial cell culture was completed, the fermented bacterial cell in the plastic lunch box was collected, extracted three times with ethyl acetate, the organic phases were combined, and concentrated to dryness using a rotary evaporator to obtain a crude extract. The crude extract was first separated by elution with a silica gel column n-hexane-ethyl acetate system and a chloroform-acetone system, and the fraction where the target compound was located was determined. The fraction was then subjected to semi-preparative HPLC purification. The present invention identified the structures of four terpenoid compounds,1 H-NMR and 13 The C-NMR data are shown in Tables 6 and 7.

[0170] Table 6 - Compounds 6, 35, 136 and 144 1 H-NMR data assignments.

[0171]

[0172]

[0173] a Recorded at 400MHz, and the assignments were based on DEPT, HSQC, COSY, HMBC, and ROESY experiments.

[0174] Table 7 - Compounds 6, 35, 136 and 144 13 C-NMR data attribution

[0175]

[0176] a Recorded at 500MHz , andtheassignmentswerebasedonDEPT,HSQC,COSY,HMBC,andROESYexperiments

[0177] The above embodiments are preferred implementation modes of the present invention, but the implementation modes of the present invention are not limited to the above embodiments. Any other changes, modifications, substitutions, combinations, and simplifications that do not deviate from the spirit and principles of the present invention should be considered as equivalent replacement methods and are included in the scope of protection of the present invention. Sequence Listing <110> Wuhan University <120> A terpenoid biosynthetic gene cluster and its expression cassette, expression vector and recombinant microbial strain <160> 141 <170> SIPOSequenceListing 1.0 <210> 1 <211> 12258 <212> DNA <213> Artificial Sequence <400> 1 atgaagatcg tcgtcgttg cgccggtctc gcaggtctct cgaccgcgct ggctcttca 60 ggttcctcaa caaaacaccg gatcatcctt ctcgaatcag ctccagcgct cgccgagctt 120 ggtgcaggcg tgcaactaac acccatcgcc acccgacagt tcaaagcatg gggtcttgag 180 ccccaactcc tcgccgcatc cgctctgcct aaggcatgga atctacgccg aggctcagac 240 ggagaggtgc ttaaccgtgt gccgatggac aagttcgagg aatggtatgg cgcgccatat 300 gtggtcgtac atcgtgcaga tctacacagg atcttgcatg aagctgtcgt agcgaagggt 360 gtggaagtgc gcttgaacag taggattgaa cagtacggga tagaagaagg atgggtcagg 420 ctaatgaatg gtgagaggct ggacggagat ctggttgtgg cttgtgatgg catcaatagt 480 tcggctagaa cgcagctttt gaagagcttc gggagcacat tggctcgaga cgaggagctt 540 gaaaccacgg gatgggcagc gtatcggctg atggctgatg ttgaagatgt caagggtgat 600 cccttgacga aagatatcgt gagcgaacat gctggcaact gctggtgcga catctctttg 660 gctcgaatct ggaatcagga gactaacggc cgtcagggcg gacgcaagca agtcagtaat 720 gacctacatg gttcgcgact ccggaaaact caaccttgtc ctatctcacc ccgatgatgt 780 tgacacaagt acctggacac gcgaacaata cctggcggag cttgtacgct tctatggcga 840 cattgatccg tgtgcgcgac ggctcatcga actcagcacc ggtcccatca cgaactggcc 900 ggtccatcaa gtcgcgaaat tgcctacctg gacgtcaaag actggcaagt ttgtacttct 960 tggtgacgca gcacacgcca tggcgttcta tctcagtatg ggtgtctcgc tggccattga 1020 agatgctacg gcgcttgcca cggctttaga cctgcatgtc aaagacccta caaacgcgtc 1080 cttagtagaa gcattggagt tgtttgagaa ggtcaggaag ccaagggttg agaagattag 1140 ggatgcaagt ctacatgctg ggacgatgct gcacctgcca cccggctatg agagagaact 1200 gcgagatcag tctgcgagaa acgatggtgc gatcgacgga gcacgggatg gagatgcgtt 1260 actcggttgg gtcagctacg gtattaccga caagacaatc cgcaaagagt gctatgggta 1320 cgatgttatg gctgcaatgc ttgagcaggc aagagcagat ggactcttgc caatgtgact 1380 accacacgat caccttcaag aaccccttaa agctaacttc atacccagca gtatcatccg 1440 gactctccgg caatcccaat aatttcacct cataaaagtc ccttgtaccc gttccatcca 1500 ccctacagac aggatccgca tatctaaagc cccacgtcgt ctggttgccc cagcctagat 1560 acacaccctc ctgctctacc ccaccaatgg tgtgcttgta tagctccggt accagcgtcg 1620 tcttgttccc atctggattg attaagatat gcccattgta tctgtcgcgg cagatgcgca 1680 actgcttctt ggcttgggtg cccagcctcg cgatgccgtc cttgcagcgg acaggcgtgc 1740 gctcgaagct gcgcgacgca cgcagatcga tggcgaggag gggtagagtc ttggagggaa 1800 agacgatgtc gaattgttgc gcggtgaggc cgttgatagc ttcgtcttcg gcgccgtcga 1860 cgacgaacat caaggtatcg gtgaggtcgt ctgatgtgga gaggtaggag ggccagtcag 1920 cgcaattttc ggtggtgctg atctggcggg ggacgacatt gggaattgca agtgttatgg 1980 cgatgaggct gaagataaag ataattgcaa tggatttcat tcagccctcg gccacggcgc 2040 tcttcacctc cttcgtatct acctcgcccg tccccaggtc gatttcgccc ttgaacttgt 2100 ccgcggtgac atgcatgaag tttgcccgta agggcgttac actcgtatac ccctgcgcaa 2160 cagcccaacc gtcattgcca ggctcgctgt tctctacact ttcatagaca tccttgaact 2220 gcggtgccca cttgaagtgc ttgtgcgtat acctcatgtg aggctgtcca cttggcgtag 2280 cagtaccgct gcccgccttg ctctctggca gcaggtcgtt gttggaccgc agctccttct 2340 tgccaagctt ctcttcttgc ttacggatct ggttctcgat gtcggcgggt gagtcatctg 2400 ccccgctggg tacttcgacg acctggaagc aggatcctga cttccaactg ttctgcagca 2460 tgtttgtcca caaaatcttc ctagcgccca cgccctctac caatggcaca ttgaccgtgt 2520 acagatgcgt gttggcatcc cagttatcgt aaaggtactg gataagtttt gcgctcagct 2580 cgctggctcc cgagatgatc gcggcatcgt ggttgcggtc aaagaaggcg tatgacagtg 2640 cgatggactt cacaccacag acagcagcct ccatagcacc gccaattgta ccagaggaca 2700 gcgagaagac tgcggtgctg ttgcggccgt agttcggtcc ggaaacgact agatcgattg 2760 gccctctcga ctcaaaataa tggaacaggc caatctgcgc gcaagaagca ggtgtcgagt 2820 caaccaagat ccattcctcc tctgtcgaat gtgggggcag cggtgtgttg tgcgttgaac 2880 cgtggttggt gaggcgcccc tcgctcgttg tctgtaaagg cgccggacga tagtagcttg 2940 gcttgatggc tttgccgact agatgtgctt tgccgatcca ggaacgctga gtgtgaggca 3000 agacgacgct gactgtatgg ccgtggcgct gtagggtcga gacgagcgag tgaacgtacg 3060 gcgaggactg atctgatggc gggccatcgt cgttggtcac ctgcgaatgt cagtcctgtg 3120 cctcaatgta ttctcaagca tccggtacgc acgaggatat gcatatggtc gtcacagtac 3180 ccactgcctc ccttgaaggg caggttgccc tcatcaccgg cgctggtcgt ggaatcggcc 3240 gtggctgtgc catcgagctc ggcaagcgcg gtgcctcagt tattgtcaac tacgtctcgt 3300 cagagggtcc tgcccaggag gtcgtcaaga tcattgaggg cttcggcaac ggtgccaagg 3360 cggttgctat ccaggccgat gtcagcaagg tctctgaaat caccagactg ttcgaggagg 3420 gcaagaaggc attcggcaag atcaacattg tcatgtccaa ctctggcact gagtcgtggg 3480 atgtcactga ggagatcacc gaggagaagt acgaccacgt cttcaacctg aacgcacgcg 3540 cacagttctt cgtcggtcag acagcgtaca agtacatcga ggacaatggc cgcattatcc 3600 tcatgtcgtc gattgctgcc ggtctgttgg gtgtcaagga ccatgctctt tacaatgcgt 3660 cgaagatggc tgttattggc ttcatcaagg catttgccac agacttcggc aagcgtggca 3720 tcactgtcaa cggtgtcgca cctggaggca tcaagtcaga tatgttcaca caaaacgctt 3780 ggtaagtgac gttcaatcat tgacagcacc acggacattt gctaacgctc aaacaggcat 3840 tacattcctg gcggttcgcc cgactggcca gctgagaaga tcgagggttt gatggcaagc 3900 cactgcccgc ttggaaggtg cgcggtacct gaggatgtcg cgagggtggt tgccttcctg 3960 gcaagtgagg acggtggctg ggtcaacggt caggtgctga ccatctctgg tggctcgtcg 4020 cagtagctac tcgcgcagtc cgactcctct gttcgcatcg cctacaccct tcaaccatgt 4080 ctcgctcgca cccaatccca gatccaatat ggagtcatga tccgaccctt tcacggtctg 4140 gctatagtac tcgaagggcg acagagaacc agaggctgcg tcttcgtccc tcttccagca 4200 gctgtcaaac cactcttgcc ctttgtgccc aaccaagcta tcagtctcgg catggaacac 4260 gtcgacgcgc agcgtctttg tggcttcaga catccgattg tcttggccgg tagcacctcg 4320 cagcctctcg aacgctctca ctgcatcgtc gaaatctttc cacgggaccg ctagagtact 4380 ccagggtatg gacctcggtt tcctcagaca cagctgtgtg tcctgaccca caccagcgtt 4440 ttcttctgcg aacagaaatg taggaacgag attccccaac tccttcacta catttgaatc 4500 gttcaggtcc aagctctgct ccccttcaat gtctctatgc tctctccgtc ctccagtatg 4560 cggctccaat ggtacaggca ctcccggggg aatagtttgc ggcaacgacg tcttcacgct 4620 gtttgcaacg gtactactca gaccggtcga catgccaacc aagggtacga gcttttcatt 4680 gacgaatctt gccagcgatg agaacttgcc gatcatgctt gctggcagca tctcaacagc 4740 ctgcagatgc ttgactccag tatgctcagg atgtacccag ggcgtgaaga atgcaacaca 4800 cggtctctcc ggatgcaaca ggtgcggaca tgtcaacagt atgttgatta ggtaaatcgt 4860 accgaacgaa tgcgccgcta ggctgacatg ctcgatctcc agatagttca tcaacttcgg 4920 tatcatatcg acataggtcg agatccggtc ttcaatcgca acagcatcgc tacctccaac 4980 acctggtcta tcggggtgga tgacgcggat cttgtacttc ctgccgagta tgtccaaggc 5040 agagtagcag aatcggccac ccatgagacc accacagaag agcacgactg ctttgcttgt 5100 tggatcacca caatcggcgt aggacacag atatggctct gttctgccgc ttacggtact 5160 cggtggtatg gtgtattgac gatggaatcg ccggtcgcat aagaactcca ggacgtcgtc 5220 aggtgagctt tgaagcgcca tatgagctcc tccaccaccc agtcatcaac acctttccct 5280 gcaacgccat acaagccgcg gtacgataag tggccataca atgcatccga tttccagcgt 5340 caagatgaga atgacgatgg tatattctac cgtcaaccac gattggtcac tcatatcgac 5400 gaccctgcaa ttgaaagact gaccgcatat tacgcaactg tcttgccaac taaagggaga 5460 ctactcgata tgtgcacaag ctggaagagc ttttacccat catcaagcaa aagtgcggta 5520 caaaagggcg acgttgaagt atatggcgta gggctaaacg cagaagagat ggcactgaat 5580 gggctgttcc aaggcaaaga gaggtggaga gttatggatt tgaataaggc gccgcatgac 5640 ccaagagcgg gttggccaga acaagtcctc agattcgatg caaccacttg cgtggtcagc 5700 attgactatc tgaacaagcc gctggaggtt tgtaagaacc tgttggatgc tacaaaagag 5760 ggcggcacca tacatctggc gatcagcaat cgctgtttcc caaacaagat cgtgaggaga 5820 tggatgatgt taaatgagca gtcaaggctc gagtttgtcg gaggtgagta cgttctctaa 5880 aagcgtttgc gcctactgac gttatgtgca gattacctgc acttttccgg gtggaagaac 5940 gtcgagatcg tggacctttg tgctcgagat gagaaggggc aaaggatcac agacgaccac 6000 gggactatta cttctgcatc tgctgccgga agactgatgg gtcacctcga tccgctttgg 6060 gttgtacgag caactaaggc ataaatgtct cctgacctga catacaccga agtcaatcgt 6120 aagcatccac gaatctatcg ttcgtttata tcgtctacgt tatctgacct caaacagaga 6180 atcttgctgc gcgagagaat gcctcctggt tctcaccggt tcgctttgct tatgactggc 6240 tcgaagatgc acccatcgaa cacctcaccg cagtgcctct gaagatacgc acagactcac 6300 ctctcgcctc gccaaagccg agtaatgtca aagaacagag catcactgag aacaacgaga 6360 acagctttag tatctcgcct cagttaaccg gcttgccctg gccgacgagt ttcaccaaag 6420 ttcgccagaa tcgccattg cgccagagtt tgcgtatctc cacccaactc ctcgagctct 6480 tcgctgccga tgacacatcc gcgcaggctg ttcggcgtaa cggcgtttcg cttgcacgta 6540 tagcttcaca tgagctccag accgacgagg aggaccgctt tactaagttt gctacataca tctttcccga agcaaatgag gagcgcatga aactactggc tgcgaccatc gtatatatta tcatctttga cggtaagcgc gcgcatcacc ggcctttatg catgcatgtc tgaacagga cgcagattca tgggaaatgc atagcgaaga tacggtaagt accccgcttc cgtatttgcc tctgtccatt gtcgcttact agagtgtgct aacacctgca tgaagctcgg tctggttcgg gatgatttta tcagacggct gcgaggtgat atcgagggca tggctgagca tcagacgccc 6900. ttgcagcaac tcatcaactc cacagttcag gggttcaaag accaggaca aactatgggc 7020. aacggtggac aagaagtact tgaccgtttg atcgacttct gtgaacatgt gcctccccag acgaaattcg caacaatggg tgattacctg agctaccggc tgattgatgt cgcttttccg tagtggttc cattccaata ttgatcttgg ctgatctcgt ccctataatg gcaggtatct acttgcgtgc atcaagttct ccctcggctc gagcgtcaat gttgaagacc cgaagcttgc gcccatttta cggctggtga gcgaccatgt ctcattggtc aacgatctgg cgtcctatga 7260 caaggaaaag cgggcatacg ataatggtag cgcatgctac cttatcaacg ctgtagacgt 7320 tgcgcagcga ctcttctcac tgccctcagc cgccgaagcc aaggcactca cgtattctat 7380 gcagctcctt gttgaagctc agatcaagac ggaacttgac agtttggtgg ctggaggaat 7440 actgtcttgc gaggagcttc gattcctcga tgctgcgcta ctcatggcct ctggaaacgt 7500 gttctactca gtggtctcgt cgcgatacgg tggtaaggca gccaagctgg aaaaatagat 7560 gttgacgtg ctctttgacc atgcgaaact actgaacctc gttctttatc tgtcaacgac 7620 tgctgtggtt cttgccatca tagatagcgt ccgcaacgtc ttctttcatc gtctgtcgac 7680 attccctggt ccacgactgg ccgccgcgtc ttcatggtgg tcggcatggc agcaagctat 7740 ccttggaaag tccatgcacc acatatgtga gcggctgcat gaacgatatg gtgggtcttc 7800 tctacaccta tcgtaatata tgaacgactt aaaagctgca ggcgatattg tgaggatcgg 7860 tcccaacagg gtatgtctga tgccctcata aactattctg gtcagtcaag ctcatagcgc 7920 ctagctgcac ttcaaccatc cttctatgta cgatgagata tatcgccaac gctgggagaa 7980 agatcctgcc ttctacggtg tcttgggtgg cgatggctca tctgtaacgt atctgaagta 8040 ccaagatgca aagtcgcgca aagacatcct gcgttcattc ttcagtagga gctccatctc 8100 cgcgcgcgaa tggatgattc aagaaaatgt acgcgacaac gttctgcttt agtacggcca 8160 gctaacagtg agaaaatcag ctggacacgt tctgcaacat cctggcacaa gaccacaagg 8220 ccggacactc gtcgaatctt tcactggggt tccgttgtgt tggtctggag accatcacct 8280 caacgtgctt tgggaagtct gtcgactgct tccgcaaaca tgacttcaat agcccggtgc 8340 tcgaagccat ggagacgata cccacccgtt tcatgatttt caaacacttc gccattgtgc 8400 gcaaactcat cctgcatgtt cctgaatggc tagccttgaa ggtcttcccc aaaggtatcg 8460 cgttcgcaca gatgagaaac gtaccgtacc taccgcctgt agcgtacagc atgcactgac 8520 acacgcagat gttctccgag caaattgata cgatcattac ccagatggag aaccctagcg 8580 aatatgaaca cccagtaatc taccgctctc ttgtcgactc agagcactac aagggccaac 8640 gcttgccatc tcgcgcgagt ctgctcgagg aggctatagc cctcgttctt gcgggtaccg 8700 atactatggg ggcgacattg acggtcggta catatcggct gctgagtaat ccgtccgcgt 8760 atcggcggtt gaaggaagag ctgaaagaag cttggccaga tctcataact cgacctcgct 8820 cggacgcact tgagaagctt ccttatctag taaatgcacc cggccattgc ttttgcgcta 8880 taatgctgac ccaagtcttg aagaccgcag ttatcaagga aagtctgcga gtcgcaccaa 8940 ctaccacctc tgaaacctca cgaatcgttc cagcaggagg agcgacaata ggcgggagat 9000 atgtaccagg caatgtgagc cttgccctat gcagagaact tgcctgcaca ttcgctaaca 9060 aggacgaaga ctgttgttag catgggtgtc ctcttcccta tgaaacatcc agatgcattc 9120 gaagacccga catcgtttaa gccagagcgc tggctttcca aaaatgcgaa cgagctcgag 9180 aagtactttg tcccattctc caaagggcca cgtagctgcc ttggactcaa gtgagtttca 9240 ataactactc ctcttagatg ttccttaact gatcttggcg aacagcctcg cttggtgcga 9300 gctttacctt gtgtttgcga atgtgttcag gcgtttcgat cttcgccttg acgtcacaaa 9360 gtaagttcgt cgcctctttt gacccaagaa gtcctctgac gttggcaggg attccttctc 9420 tgaccttaac tggattgatg cgggtgttgc tctgtttcaa ggtgaccact tgcgatgctt 9480 ctgtagaccg gttgaccatt aatcaagtga tgacgagctt ggccccgtct cctatcgcct 9540 cccagaactc ttttgcgtta tccgcaccca tcatcgtttc catccattct ccatccatcc 9600 agccttggac gaagcatgtc ccaaaaagtt catacgtgcc atcttctctc tcacgcaata 9660 gaaggggcac agaacaaccc gccacgatgg cgagttgcca gcttgctgtg ttccagccgt 9720 ctatgttctt ctcattttcc tcggcaatat acgcaggcgc aaggcccata tacccattgt 9780 ccgttacaaa tggtctacgg ccccgcaata ctgtattgac cacataccag gcttcagtac 9840 gatcgtaaga gtttgcgtat tttgagacat gacgcgaggc ttctgggaga cagacgtagc 9900 gctcattacg tatgcaatcg tccgggtcgt atccgctgtc ggcaaccgta ggccatgctt 9960 cgatgctgtc accgtggaac atcgcgcacg ccgcacgctc cgggtcatca tacgtgtgga 10020 tgccgtgcca gtcgtgtgtc ttaatcctat cctcccaata gtggaatctc caggttgact 10080 tcatcgcggt gcccgcaggt atgtccggag catcgccaaa catgaatttg atctgatcga 10140 tgatcaggcc agtacagtga agggcgttgc cttcgaaaaa gaaaggtgta ctgccggcag 10200 cttcagcgat cccgtctgca gaatacaatt tgccactctg gtcaccgatc acagacatgt 10260 agcgcgttgg cagatagtct ttccggatca gagaacctgc aatcgctggg acatcccaat 10320 ttggacacca agacggagca ctctcgtaaa atgcagaact ctccttggtg acttccacgg 10380 caccaagaat gtccagcgac ccgtgctgga atgcccaagc ccgggtgaag actgtgtata 10440 cctgttcgta ggatttgctg tactcgggct tgaaatccag accagtcttg tctcgagcca 10500 gattccacag tgcgtagata cggtctcttg ggtcggtact ttcgaagtcc ctagcaagta 10560 caagtgcatc caagaagcca ggcgaagtct ccccgaactt gttccacaag cccccaagaa 10620 aactgcctcc agcgaagcca ttactcatca ggtagcgaaa gtagtatatc ttgcgcgcgt 10680 aagctagaaa tcttgtgtcg agcaacccta tcgtgctatc cgcatcctga atatgtgcag 10740 caagggatgg atcacgtgtc aacatgtcgt gcaagttgta tgctgccttg tctagcgtat 10800 caaaactgac agcctttctg caaccaccaa caatcaccgt agcattggat gcccacgcga 10860 cctcctatct ctcggttagc ttcgaactgt caatttggtt taggaactca cttgcaagat 10920 ccaagatctt cggaagtact gtcgagtcaa aaatttgtac aaagccgcgc acgatcgtgc 10980 caatttcgct ggcttgatag tattttcacc ataccaaatg ccccccttgt ctgcagaacc 11040 ccaattgcca aagtgaaatt caccagcgtc attaatccgg acgaaagggt gtttagtgag 11100 ctcgtccatg aagtctaaag cttcttctgt gtcatccgtt ttgggtccga cataactata 11160 gacagacatt gcattgtcgt agatctggcc catgcgacgg acctgcttgc cacgctcggc 11220 caggtcttcc tgattgatgg agagcgcatc aatccaaagt ggcatgatgg tctctttcat 11280 tcggagcgca cgaattgcat ccgcaagatt acggcgaatc gatttcatct gcccgttgag 11340 tacgacgtac tccggcgaaa gatctgtgcc ccaccgatct tgtagccatc aagtcaatac 11400 gtgatgttcc cgggcagaga agcacaactt acaagacaaa gcaataaagt tgacctcaca 11460 cttgatcgga cagtgagcca cgtgcatcac taacgatgta gtagggtctt cactcggcat 11520 gacgcagatg actcgaatct catccgtgac catatccagc ggcatgtact ggtactgcac 11580 gctcggtgtg tcattgtccg cccgcgtacc caggcgaatg gggcagactt ggggcaacac 11640 aatttcgtct cgatctgagt accattcttt cgtccattgc gcgtaacggg agtcggtgac 11700 cttctcaaag tagccatttt cgatcaaacg gctgttgacc ttgtgcatgt cgaagacttt 11760 gtaggcagct gcgtacatct tgtcgctgaa ttcccgcgtc caatatgtcc tctgttcatg 11820 aggattgaac tccactacgc atgcatagcg cacccataac cgcgtgggtt tcttgatcct 11880 agtccgcaaa taccgaagaa accgctccag cgccaccgat attaccaaag ctttgccatc 11940 aacttcgatt gcttcttgga ctttacgata acctcttgcg ttcttgattg caatgaatgg 12000 gggaagctcg gccaacgggt gaatctcgag attgcatcgg acatgcccga gagcttcatt 12060 ggtgggatgc agcgtgagta gtcgaacttc aggcgactcg acattgagcg gcgtgtactc 12120 gatctgaggc acaacaaacg tagattcttc atcgccttga acgtacaagc tgttgttgag 12180 gtcgcgcagt ttctgatccg ttttgcagct ttcggtcagt gccacatctt ctgtgtcgag 12240 ttcggtatga ggttccat 12258 <210> 2 <211> 11832 <212> DNA <213> Artificial Sequence <400> 2 atgcgctctt ttgctgcagc aacacgatcg atcgcacgcc agcagacaac ctgcagaaac 60 agcttccagc gccagacact gccctcgatt gtatcaagga catattcgag caagatgggc 120 cacaacgtgc ctgccgtacg ttcctagcat cccgtcccat ctgtgtccgg acgtgacgag 180 caacagcgct gacactccca cagctcaagg acccctccct tctcaagagc cagacataca 240 tcaatggcga atggatagac gccaaatccg gcaagacctt cgaggtccac gatccagcga 300 ccggcaagct catcggcacc cagcctgaga tggaccgcac cgacaccgaa gccgccatcg 360 ccgctgccgc agctgctctg cccttgttcc gcaagttgac cggtcgcgag cgatcgaggc 420 ttcttcgaaa atggtaccag ctgatgaccg agaatgccga cgatcttgcg acacttatca 480 catgggagaa cggcaagccg ttggcggatg caaagggcga ggtcacgtac gcggcgagct 540 tctttgaatg gttctctgaa gaggcaccga gggtgtacgg gaataccatt ccttcgagcg ttgccacaaa cagagtattc acagtcaagg agccagtcgg tgtgtgcggt ttgatcacgc 660 720. cgtggaactt ccctgctgct atgatcactc gcaagatcgg tccggctctg gctgctggct gcactgttgt tgcgaagtct ccaggcgaga ctcccttcac tgctgcagcg ttggccgagc 780 ttgcacaccg cgcaggcatc cccaagggtg tggtcaactt tgtcacagct atggagaaca 840 cacctgaggt tggtgagaca atcacaacca gcaagaccgt caagaaggtc tcttttaccg ggtctactgg tgttggaaag cttctgatga agcagtcgtc gtcgactctc aagaagctgt ccttcgaact tggtggtac gcgcccttca ttgtcttcga cgatgccgat ctcgagactg ccgtaactgg tgctatcgcc tcgaagttcc gctcttcagg ccagacctgc gtgtgcgcca 1080 acaggatcta cgtacagtcc ggcatctacg accagttcgc tgagaagttc actgagaagg tcaagggctt caaggtcggc gcaggtttcg aggagggtgt aactcacggc cccgtcatcc 1200. acgaccgcgc agcgaagaag gtcgacgacc atgttcagga tgccgtcaag aatggcggca 1260. aggttctgat cggcggacag aagctcccag agctcggcag ctctttctac caacccacag 1320 tcatccgga catgaccgct gacatgcagc tcgcgcacga ggagactttc ggccctgtcg 1380 ctggtctttt caagttcgat accgaagccg acgtcgtcaa gctggcgaac gcagcggatg 1440 taggtctcgc cggatacttc ttcagcaagg acatccagag ggtgtacaga gtcgcagagg 1500 cgctcgaggt gggtatggtt ggcgtgaata ctggtctgat ctcagaccct gccgcgccat 1560 tcggtggtgt caaggagtcc ggcttcggcc gtgagggcag catgcttggt attgaggagt 1620 atgtcacaac gaagacaatc accatgggcg gcaacgggca gccgctgcag ggcgagtaa 1680 tggcttcccc tccggccctg cgcctgaggc cggtccagaa gccggacaag tatcccttga 1740 cccaagaaga catcgtccgc tcaggtccta tgacacatga gctcttcggt ttgaagctct 1800 tccaacacta cgtcggatgg caaagttcca tccttggcta tggcggtgcg cagtttcctg 1860 cccatgcagc ctaccgacag cggctgcttg agacgtgcaa ctcgaacatc gtcggcctcg 1920 ttccctcggt agagcactac aagtgcttcg tcgagtacat gtttgcaggg aagggcgcga 1980 acatcctatt ccctcgacgc cagcagccga acgtctcacc aggagcagc gtaagcgctc aaacacagcc aagtgctttg ctctgcggtc accgactgca ccccagcaac aacctcagct tttccaaggc ctgcccttgt tgtcttctcc tggagatcaa cgcagcgctg gcgcgtatcg 2160. acaaggtctg gcacaccttc gaaaacaaag gaagccagaa gcgtatgtac cgccattttc gaaggctctg gaatgtgcag aaacagcagt ggaggcattt ggttgatagg atggagctag cggccgagcg ggagcgtagg tgggagttcg agcagctgga taagggccgc aagatcgaag 2340. aacttaccgc ctgcactatg agttgtgccg acctgctcag cgttatggac gccactccgt 2400 2460. gcatggtctc gggatggac ctggcgttca tccccaagcg catgcgacga ctggaggtct gtaggtcttc tgtgtcacga gccactaaga agcagateac aggatgta atgtactcaa cagagtatac attcatcccc tcacctgcct tcaaaggttc gaggcatagc cggacgagtc aatacccagt tgtgggtaag atccacggct atactgcgtc cacagagcgt cgctccagat ccgactcacc cgacaccatt acatcctcgc cgtcaccttc gacctcacat gctgtgcggc tcccgcgaaa gaaaacctca ttcgcaccca ccgtcatgga gaacgagccc cggccacgcc tatccttcaa tagacgctcg gacacctatg ctcccgggcg atggacttgt cgcgaaggct acgagtgggt cgactcgtac acgcggtaca aaccgggtcc gacatcggat gaagaggttt gggaagagga agaagatcag gaatggtgga cgccgcattc agtcgtcgag gacggagatg actacgagtg gatagcagac gaggacctga atggtgagtt ctggacga gagaatcgg atgatgagga cttcgacaag rich agccgctgac tcctgagggg agtcagactc ttgggccgga cttcaatctc acagcttttg accctgttat cctcagtatc gagggggaga atggcttcga agtcggcgcc atttctcaag cgttgttgga cattcgaagt ctaaatgcgc cgaacatctc caaaatcgac gatgattctt atggggtgta cgacgagtct ggagtcaagc gccgccgact caactagatg tctgtgatcg caatcacctc cctcacaacc tcgaccccag 3360. tcgacgacct cctacacacg ctcacccaag ctctcaaatc ttcccacacc aacaccccct tcgtcatcgg caccaaagtc caatccccct ccaccatcca aatcacctcc tcatggccac ccccacca ccccaccacc ctcaactcct ccccagcatt ccaagccttc 3480. 3540. tccgaaccac atcctcatcc caacccacca ccatcacagc cctcctcgac acctccccct tcgcctccgg caccccgccc ctcgtcgaat tcgtacacac ctccttcccc gccgcggacg 3600 taacgcccga agtccagcat cgtacggagt ctgactttgc gaagttcga gatatttatc gccggcgcgg cacgatgcag gatacgggcg aggtcgggct tgcgacgggc tggacggagg 3720 agcatgatgg ggtgagaggg tttttggttg ttagggggtg ggtggggatg cagaggtttg 3780 aggaggcggt tgggacggag gtttggaggg aggggattcc gatcttattg gggtggggga 3840 agccttttga gctgtggcat gttgagagga ggggtggggg gaatggagcg taaatgactt cagcgaacaa tcgacaaaac tcacaaccgt cggggtgtga gccaccctac agtatattca gcaaaaaaca aaaagcactg atcgtactta tcgtatccac cgcagcgacc tgtaagtact cgaccctttg atcgttgttc tccagctaat catgccgtag tctctggctt cgcatcgaac atctacttcc ctgcgattcc atccatcgcc cgcgacctga aggtctcagt ggagttggtc 4140 aatcttactg tcacttcata ccttatcttc caaggcctgg cgcccagcgt gtggggtcct 4200 atttcagacg tgaaggacg ccgagtggct tactttggca cctttgcagt cttctttggc 4260 gcatgcattg gcctcgcact gtccaaaaac tatgctactt tgctcgtgtt aaggtgcttg 4320 cagagtaccg gcagtgcaag cacaatcgca attggatcag gtgtcattgg cgacattacg 4380 acacgtgcgg agcgggggagg ttatatgggg gtgttccaag ctggactgct tgcgccccgtg 4440 gccataggcc cggttatcgg tggtgccttg gccggaacac taggctggag atcagtcttc 4500 tggtttctga cgatctatgc cggtgtgttc ttgttgctcc tgagccttt gttgccggaa 4560 acactcagga ccattgtcgg taatggagat catgctcctt caagcgtgat ctccagatat 4620 cctctggtgt tctaccagaa aagtatgaaa gtgccggtca acgactccga aagaattgcg 4680 ccacgaacta aaaagaacgt cgacatcact ggacctttgc gaatcctgtt cagcaaacac 4740 gcatttccca tcatagtctt cctcgctgtc tattacgcgg tttggcagat gagcatcacg 4800 gccatgtcct cgctctttga gagccactat ggactcggcg agacccagat cggcttgact 4860 tttattgcga acggtgttgg atcgactatc ggtaccttgt tcactggaaa gattctcaac 4920 atggattatc gtcgggtgaa gcaagagcac gaggcgcaga actcgaatcg caaacctgat 4980 gaggctacga ctgactttcc actcgagaaa gcccgacttc gacttgtgcc actgttcgca 5040 ttgatacaat gcggctctat cctcattttc ggctggacca tcaactatcc tcagcgcgtg 5100 cacatcgcgg tgcctattgc ttccacgttt gttacaggct ggacagctgt ctcaacgcag 5160 tctgtagtca tgacgtatct gattgatgtc ttctcagatc gaagcgctgc agcaagcgcg 5220 agtctgaacc tggcaagatg cttgtttgcg gctggcggca caagctttgt tatgcctatg 5280 gcgaatggta tcggcgtggg ccttgctttt acagtctgcg cgattgtgca actaattgca 5340 ctactgggag tggctgtgca atggagattt gcagggaagt ggcggaggga ggatgaggga 5400 agacggagca ctgcagcggg agaaaacagc tcaccagcat gatcatccga tcgtgacccg 5460 tatgcctttc gtatccaact tcggtagcgc aataatgaag tcatgcaccc gagtcacatc 5520 agtctcatct gtctggtaca gctcaagctt tggagcttgg ggtcgaaaga gcactgcaag 5580 ggctagatac aattcagcct gcccaagact aggtgtcgcg tattagctag ctactgagtg 5640 ctgtatctgg agaagactta ctgctgtcct agacagtttc gcgatcctct tccgaatggc 5700 acatagttgt ggagcatcgc aggagtgacc gtacccagcc acctctccgg cctgaactca 5760 agtgggtcgg gaaagacgtc cgggtctgtg tgcatgaagt agtttgacat gcccaccgga 5820 attccaggcg gtattgtgta tcgtttgtat tgtatggcca catcaggaga gattcggggt 5880 aggcggtgca ttacgccgta tgagagcctt tgcatcgtta gttatcgctt tgcgcctaat 5940 tgaagtgagg tacgtgccgt agcgactctt tgatggtagc tgtgaggagg ggaagtttttt 6000 caagctcggc ttgagaaggg atacgggagg gccagtctgc cattacatct ttcagctctg 6060 tgcagaggtt ctgtaggagc cgtggccgac aaaggatttg aacagtcgca aacctagtat 6120 tgtattagct ctacgtgttt gtttttggac tgaacactaa ccccagagta tgggatgtgg 6180 tggctgttcc tcctgcgagt agaacctgtg cctctttgag tagcctttca ggtgcccttt 6240 cgctcgctgg catgtcacta ttggcgagat gtaggaagac ggaaccacca ttctccgttt 6300 tgacaccctt ccggtcgttg tcctctttca cgcgtacagc ttcatagata catgcgagtg 6360 agaacttccg aaagtctccg aagactttcc cggcaggcat cagagcaagg agaatccaga 6420 ttggaatgct gtttacgatg tgtatgatcc agggaaagcc gctgaaaagt ggaagagatg 6480 tgaccatgct gttgatacag ttgaaccact caggcgagaa gtctgggtga tctaagaagt 6540 ccagccgctt gtcactgtcg cgaccgttgg cgaggcataa ccgacacatc acatcgctgc 6600 tgaacgcgct gaaaacgtgg tccagtcgca caaccttgtg tgttcgttca aattcgcgca 6660 accggatctc catcttcaga actactgaac caagcatagc ctgtaaacgt tgaacacccg 6720 acctggagaa gaatgggtct agaggtttgc gtcgttgacg atggagctca tggtcgcgtg 6780 tcaatatgtg cgatcctatg tatatcagct tcacacaatc gatgacaggg taaaatttag 6840 tcagcatacc ctcgaagtca atgccactgg cgaaagcatc atagtgttct gaacggcgct 6900 tgcttcctga aacatagagc tcgttgtaga acgaggagtc gcggatcgac aactcttctg 6960 ggttgactcg aatgatcgga cctttacgag gggttattta gccaagtctg gtgctaatct 7020 caatcacgat gcaagacaac tcaccataca catggtgcat cctctcgatt tcgaagatgt acttgccttt atgaaagaag tcatagtaga actcgtacca gcccgtggct gcagcgagtt gagaacctgg gaacctcgat agaggtgagt agtataccg ccagatgaca aggtatgcaa agtgagctgc cccaagtgat acaagaatcg ctgtaagcga taggtagctg agtgaaatgg tcatatgcga taccaatatt ctgagagggt ggagagccat cgatatcgtg acgatggact ggccaacaac atacatctac gtatacacaa ggattcttac aaagaggtca ttgggacatt gcgggctcag aacgattgga gtcgattggt cagttctatg acgaagtacc atggcgggct 7440 gggcgacctg ttcagcttca tagcgtcac cattccagaa tgcttaccag agcgcctcga agtcgtggcg tacgcaaacg agtatgcctt tctttacgat ggtgtgtcac agctgatccc 7560 gcgatctgct gcctcttgtc gtgtatctga tgctttgcag atcaaatgga acgactggat 7620 ctcaaagatg tcagttcgag agacgatcca tacaacagca ctgttcactg accccatcag tttcgcgaag gccgggacga tatgctggac atatttggta tacatggtgg agccagcaac ctcgaggacc gccgacctga gaagaccctg cagctgcaga tcttcgatga gttgatggct 7800 attgaccagg atcgtgccat cgttacaatg caagcttggg caaagttcat tgaccttgca 7860 tctcgaacac gcgtagagcc cttcaatact ctggctgcgt acctgccgag cagaaccatt 7920 gatgcgggcg agctgtaagc actcaacagt ctcgttacac aggatcgtgg tccggctgac 7980 gtattagatt ctggtttggc atgctgactt tcgcaatggc gttgacgatt cccgcgcacg 8040 aacttgatgt atgtatgcgc ctggctcgac cgggctatga agctatcagt ctcatcaacg 8100 atatatattc ctggcccaag gaacgtgctg aggcggagaa agctggccag gattacgtct 8160 tcaacgccgt atgggtcgtg atgaaggagc ggaaatgcga cgagcaaaag gccactgaat 8220 tctgcaagaa tctagcccgg caatcgattc aggacttcag caaagtgtg aacacaccgc 8280 aggtcacgga gttatcatgt gacagtcgta cctacctggg agctgtgaga ctgagctatg 8340 ttgggaacct tgtgtggagc atctactgtc cccgatacaa cattgcgtaa gccccaatat 8400 ttgccattcc actgacgagc taacgtaagc gcagggtacc agtctaccac agtaagctat 8460 agatgttcgt tcttagcgcc aaactgcggc gcaatctggt gttcagcgct gctttcatca 8520 tgttaatgtt ctttgtcgct caactcttct ttggccctga gcgaacggtg cagggagtga 8580 aagacacaac tcacaagtgt gttgttcctt cttcacaaga aaattggagt tcaaattga 8640 caccgaaaca ggatcataaa agctacacaa cagcaaccac tgtccgccac agcctctatc 8700 caagcgctgt ggtcttccat caggcttccc gtcactgcaa gaacctacac cgatcctaac 8760 ggcaaacaat ttaacctcac ctcaaaacca tcatggacga agcccctcgg caaagacgtc 8820 ttaatcatcg acatcgacac ccgccagccc acgggctaca atcagatcct caacaccaca 8880 aaagtaaact gggaaaagct tggagacggc acatacatgg acgacaacat cggcctgacc 8940 tcctctgcaa ttttcaacca ctatctatac tcacagattc acggctatga ctacgtcttc 9000 tttcaatccc aggaaattgg ttatgtgcac cacacatggg tcaaagtgca tgctattcct 9060 gaagtgctca aaaactacaa gttcgttgta ttcctcgacg cagacgtcac tgtgcaccac 9120 atggaagtgc cccttgagtg gctcttcaac cgctggaaga tcacgcctca gacttcgcta 9180 gcgctcccgc aggatgtcac acgcgaaggc tgcgatacct gcgattcaaa gggaaatgtc atgttaagct ctggcgtgat tgttgcgcaa aacatccctc atacgcataa gattttcgat gagtggaag cgtgcccgaa tgagacgaga tatcctgggt gcggggagtg ggcatggaac 9360. tgggctcatg agcagagggc gttgtccgag ttcctccggt acgacgagg gttgaacccg 9420 aaccagaaca tcttggtgag tcttgcaggc accttcttac ggagtttgcg atattgatga atttcggcag gcgatcaact gcgacgatgc aaacggctat cccgagaaaa acgaagtcaa gcccgacggc atgatcttct attccgactg ccagggcaac tttttccagc accactggct cgacggtggc aagaagctta ccaagaaggc tatcggtgaa tcggtcatgc agtccatcgc tgaggttgta caaaagtcgt tatggggaggg acgggataat ctggtgatta atcagacggc ggaagcgttg gcagaatagt tacgggtacc atggaagtgg agcggaggtg gatccacctc 9780. gactggagac cgagatccac ttcggagcgt tagactttct gggcaggcta ctcactgcct ggtacagcac gacgccggac aagacgttga tgttgaagct ctcggtgaag tatttggggc cagctatctc gaggggagca gcgccaatag agacgccgga gttggcctcg acgacatcga 9960 tgtgatcgat tccagcagct tcaatagcct caacagcttt cttgggatcc tcaacattcg 10020 tgttctcgac accaacaagc agttgacgtg agccgtttgc tgtgggcagg ctcttaagtt 10080 cctccgcctt cgaagccttg ggtcgcgaac agtgctgatg acaacgtgat ctggcttcaa 10140 gagataagtg gctgctaggg cttttccgat gcctgcaatg gagcgatgtc agagtgatga 10200 gaatagatgg tagtggtcat ccccgaacat ggtctggaac gtaccttcgc cgacgccagt 10260 gatgagaact actgttggag gagccattta acctctcacc cacgggtcct ccatgtgcac 10320 ctggtgttgt ctctctatct cctccaacgc ctccctctcc ttcattctag cctccttgct 10380 tgcgaaaact gtggcttcga tatcgcgcac ttcagcctgc tctccgtcat atagaccaga 10440 ccaaccaaca tcgttcttga ttgcactaca atcgatccta cccagcacat gggagcttgt 10500 gtgcagatgg gtccacgtat tactcttgac ggtgaagaat ttccagtcct cgacaaaccc 10560 ctctttgtct cccctgtagt cgtagccttc gtacttcaca cccgcagcct cagctttctt 10620 cctcatatcc tccgcataga aagagttggg actcccttct gcctctcttg caagcgccct 10680 atcccatggc gccccaatct cgcaatacgc atccgagtag tctgctacaa cgcaagcccg 10740 cgcagcttgt acacgcatat ccggccgcct cctcgcgagt tcgacgtacg tatccggcac 10800 cgcgaactgc ggataccata tcaagtacgg cagctcacct cctttcgtgt ctacagtaat 10860 gcgctcgaga ttgttactca tgacgaatcg tgcatggagc gctttcttca cccgcagcgt 10920 gtcgaagcgc gaatcaccag cgtcgagctg ttgcgccacc cactttgcga aaagagggtg 10980 gtggtagatg ccgtggacaa ggcaggggcc ttctttctgt ataaggaatg gtcgacgcag 11040 gcgcgcgtac cgatcgatgt tgccttgtac agcggccatg aggattagga ggtctttttg 11100 caccgtgggt agatcggcgg ggagcgggtt gtacagcagg gggaccacga tggactgttt 11160 ggtctttggg gcgctgacgc tgtaagtgtc gatggacatg tcttctgtga tatcgaaata 11220 gcgctctctg aagccattac gtcggtgcca agatgacacg ttggatttga caggccaggg 11280 cgtggtgtat cgctgcttga cctcaagcat ggagcgtaca cacgtgtctc cattgatgag 11340 agaagggcgc ggagagcatg gtgtgtaggt tcgcgtgtag tcgttgaaga cagcgtactt 11400 taccggctgg gaagtgattg cattatagat gttcaggttg ttgttatcgc gtgcttcttc 11460 agcgatatga gcttctggga gaagggcaag tgagtggtag aggtcagtgt agccagctac 11520 agcgcagaca cggccaatct ggtaaatcat ttgcgggtat cgttgtaaga gcgctcggca 11580 agtgtcttgg ctgggcgtgc ttggatgcca gatgcagtac ggaaactcgt catggttaag 11640 tatgtccggt atgcgattgt tcatgatggc ttgagcattc agagcgcggg taacgcgcga 11700 gtccgttccg tgaagctctc ttgcgaagcc gtcgttgtat ctgatgcctc ggatgatcgc 11760 tcgctgagca agctccagat ccatgtttgg gggaattttg actccaaata gcgcatcttt 11820 gatccatgac at 11832 <210> 3 <211> 19793 <212> DNA <213> Artificial Sequence <400> 3 atgaataatc aacttttact ggccgtggcg gcgtttgccc tatttttcta cgttgctgca 60 gcattgttca attgggtgcg ctctcctttg cggtccatac ctgggcccat gttggcgcgc 120 ttcacggact tttggtattt cttccgaatc aagcaaggca gtttcgaaaa ggtcaacatt 180 tctcttcatg agaagtatgg tatgtctatg tgcagagatg tcaggcttgt ctgcgtggca 240 cttgctgatc ttgaggtgca ccccaattag gccccatcgt cagatacggc cctaatcgat 300 acagcatcga tgacgctgag gcatccaaga ctatctatgg tcatggaacc cagttcccta 360 agtcgatctg gtactcatca tttcagccaa atgacacttg gtggaacatt ttctccgacc 420 agtccatcaa acgccacgct cacaacagac gcttcttcgc gcacgccttc tcgatgacat 480 cgctcgttaa ctacgagccg tatcttgatg agtgcggcga gatcttctc caacgcatgc 540 tggaattctc aaaagccggt gcacctgttg acatcggcca ctggtttcag tgctttgcct 600 ttgatgccgt tgcatatatc acgtatggca agcgactagg cttcctggac cgcggcgatg 660 atattggcca ggtcatcgag aaccttgacg ccagcttaat ctactcaagc ctcgccggaa 720 tttcccttc attccatcgc tatattgccc cggttctctc agccctgggc cctctttc 780 gaactgaccg catcatgtat gtgatgaact tcactcagag cagggtcacg gaagagcgag cttcgcccaa gtcggtcgct gagtccaaga ccgacgtaga aggtgcaggt gtcggtgaga ccttcttgag aaagtatctg gctaaacact ccgaggaccc cgatgctttc accaattacc acattctcat tggctgcgca accatatgt ttgcgggctc agacacaacc ggtattagtc tttctggaat cttgtaccat cttcttagga acccggattg cgtcacaaag ctgcgagccg agatcgacga tttcagcaac cgtggcgagc tctcggaga tcccacgttc aagcagggtc aggacatgcc ttacttgcag gctgtcatca aggaggcgtt gcgtatgcac cctgcagtcg gcctgccgct ggagcgagtc gttcctgagg gaggcgccac catcgccgat cgtttctttc 1260 ccgaaggtgt acgtactgat gacttttgag ggaatcggtc attttactaa cgtcttcctg tcctttttag tctgttgttg gcatcaatag ctgggtccaa catcataaca agagtatctt cggacaagat gccgacaagt tctgccctga gcgttggctc attgacgacg aagacaagct ttccccgatg aaccgaaact ggatgccggt aattgcggtc ttccaagctt ttgagtgagc ggtttggcta attttggcat agttcggctt ggggtcaagg acgtgcttgg gaagaatgt 1560 ttccattttg gagatgacga aactgatccc gagatcatc cgcgatttcg acttcaagct tgacggagac gcggcagcac ctggagggtc ttggacaact gccaatgcgt ggttcgtgaa 1680. gccccgaggt ttctgcgttc gggtagcc ccgaaagctc agcgcttgaa tgtctcaatc 1740. ctacaaatca agcagccttt ttgatatccg agggatcgta gctgtcgtaa caggcggagg cagtggtatg tccgcattca ccataactct ttaatcattt ggctggctaa tcacatttgc tgcagggcta ggactttaca tgacgcgagc gttggcagaa aacggcgcaa aaaaggtcta catccttggt agaaggaaa acttgctgga tgatgctgcg aaagaattg tgcgtaccgt ccaatggtcg ccaacggtcc aacgcccgct gacttatagc agggctgtca caacgttgta ccgatcccat gcgatgtgac ggatcgccag agcctcaaag cggcagtggc gatgattgaa aaagatgttg ggtatattaa cctgctcgtt gcgaactcag gtattgccgg accttctgga aacgtgcctg ctggcgcctc gattgcgcaa gttcaagagg atctcttgga gattcctatg gaggagttca ccaacaccta tcacgtcaac tgcactgctg tattttacac ggcgatatca ttcctaggcc tcttgcacaa gggtaatcag tccaattacc agggtggaag gagccaggtc attgccacca gcagtatcgg cagcttcaac aggaagatca ctgctgggtt tgcgtacggc actagcaagg catcaacgac gatgatgttg aaggttttgg caacgtatct agtgccgtac aggatccgag ccaatgtact ctgcccggga tgtaagttgg cgtccggcaa acatagtttc tggtcaacga tgctaaaaat gtcaagtgtt cccaactgac ctcactacga gtctaattca aggcacggat cctacaattg agggggcctt tcctgtggat caatacctg ctgagagagc 2640 aggtctcccg gaagacatca tgggaccgct actgtatctg gcttcacgag ccggcgcata ctgcaatgga aactgcgtct tgacggatgg tggcaggctc agcgttactc cggctacgta ctagatgaag ttcaacacgg tcgccatcta cttctccgcc atcatcggcg cgtccatggc tgctgccatt gagggtaagc ggacgaagct atgaggtcaa cgtgctatga ccccgagctg accttgttgt agagcgtcag gcaccacctg ctcccatgcc tcccgcgggt gctcctcccg 2940 gacctactcc caaggtcatc ccagtccttg tcggcagccc aacgcgcgag aagtcgttgc 3000 tcttctaccc tgagatcgtg agagctcagc ccggcgacat cgtccagttc caattctggc 3060 ccaataacca caccgtcacc caggctgcga acgttcaagc cccatgcctg ccgcttggag 3120 atatgatgcc caacgctgtt cacagcggct tcatccctgg tgtcactgac ggctccccca 3180 tcggaacctt caacgttcag gttgagaaca ccgagcccat gctcttgtac tgtgcccagg 3240 gcatgcactg ccaactcggc atggtcatga tcatcaaccc gtaagacact catgttcaga 3300 gaagcgtgcg agagaagtac tgacagcctt gcagtaaggc ggacgcggac gtgcaggcct 3360 acaagatggc cgccaaggga tcgcagcaaa acgtacccgc aaagaacgtg gccggtggct 3420 ccgccggaag gatccccgcc aacttggccc tgccccctgt tgcttaaatg gcgtcttccg 3480 gggcatattc cgccgaaacc atgtaagttt gcggaggtcc ttagcggctc ggcagcgggaa 3540 cacctgctca cgagtgacat cacaccaggc cggtcaacgc tacatcagca ctgaggacac 3600 caccctcgga gaaaacgggt aaaccggagt cggaacaaggg cgcccaaagc caagacgaaa 3660 taatcgaagt ctatcccagc ggcccggccc ttacggcgat ctcgattggt ctatttcttg 3720 ctattctctg ctttggccta gtaagttctc gacagccaag caactcttag tgttgtctat 3780 ttaccgatac ctcgtctccc ataggaccga tcaatcttgg ctactgccat tcctaaaatc 3840 acttcagagt tcaactcgct gcccgacgta gcttggtacg gctctgccta cctcttcaca 3900 caatgttcct tccagcttct cttcggaaag ctctatgccg agtacagcat caagtggatc 3960 ttcctgatcg ggctggccat cttcgaggtg gggtcagtgg tgtgtgccgc ggccccgagt 4020 tccgtcgttc tcatcgtcgg acgagcggtt tccggggtgg gtgcggccgg cttaatgacc 4080 ggagctttaa ttgtaggttt cgggagcttc aacggccgcc gcaaacaccg gctgacaagg 4140 atctagatca taaccaagag tgtaccgctt cataagcggc ccactttcac cgccgcgatt 4200 ggtgccgctg ccggagtctc tcaggtcatc gcaccgaccc tcggcggcgt ctttgttgac 4260 aaggctacct ggagtatgat tttggttcct ataatttgaa tgattttctg tttcctgact 4320 tgtgacagga tggtgctttt ggatcaatct tcccttggga ggtgtgactt tcgctgtgat 4380 cctcctcttt cttaggcttc ccgaacaggc aaagaaagaa ccaagaggct tcaaagagtt 4440 catggacaac tttgaccttg tcggaaccct gctcctcatc ccatgggtca tctgtcttct 4500 actggctcta cagtggggcg gaaccgaata cgcatggagc aactggcgca tcatcctctg 4560 ctggtgcatc ttcgcggtct gttttctttt gtggggcttc gtccaatatc gcgagggcga 4620 caaggcaacg gttcctcttc gcatcatgtc tcagcgaaca atgatcgcgt catgttggac 4680 aatcctgctc ctgttctctc ttctcttcat cgatgtgtac tacatcccga tctggttgca 4740 ggctgtcaaa aaccactcag cctacaagag tggtattgac ctcctcgcat catcagcctc 4800 gatgtctgtc gcgacaatcg tgtccgggat cttggtatgt ccctccgccg ttttgacgac 4860 gttgacactt catactgata aatcattaga cgagtcaaat tggctactac gtgccgcaaa 4920 tcatcgcgtc atctgtcatt tcctccgtgg ctggtggctt gatctattcg ttcaccgtcg 4980 acactgacac ggctttctgg tatggcctgt tacgttttcc gattcagtct gcccaatgtg 5040 ctaacatctg cctcagggct gcttcactcg tcctaatggg attgggagtg gggctgggag 5100 ggcagcagtg catcattgcc gcgcaaaccg tgttcaaggg gcgcgacatc gccctcgcta 5160 cttcggtctt agtgttccta cagagcctgg gcggtactgt gtttctggcc gtggcacaaa 5220 acatcttcag ctcgcgactc gtcgccgagc tccgccgaaa cgtccccaac gtaaatccgg 5280 ccgtggtcat caacgccgga gcatcggggc tggtggactc tatgcgcaag acctaccccg 5340 attcggtgga cggcatcatc gagtcgtaca acaaggcact acaaaacgtg tttctcattg 5400 cgactgttct cggatgttta acagtgctcg gaattgggtt tttcgagtgg aagagcgtca 5460 aagacaacaa gccgaagacg gatgtaaaaa gccaaacaga cggagatacg gagaaataac 5520 tactggggta tcatgttcgc catggccatc gtctcagtcg tgctcatccc atcctgccac 5580 atgatgccct gcaaggtctg catcgaatcg ttgccaaact gaaagtcata aggcatctga 5640 ccgaacccat ccacaatcat ctgcccccaa aacatcttgt ccatggtgac atcatctacc 5700 caagacatgc ttgggtcgtt cgttgaaccc tccgcggtgt tgggtggttg ttcagcagac 5760 ggctcccgct gcggcggaga cactttgctc aaataccaat tcttcatcca tcgtgcacgg 5820 taggcgtagt tcgcccatct agatgtttgg tcttcgaaaa gacgcctctt ttgaccgact 5880 atgagtgcaa cgttgtccgc ctcctcgaat ttgtctgcca ttctttgtgc attctctgca 5940 tgtgggatct tttgacgagc gatggtaacg tcccagtcag ggctgccgtc ttccaagagg 6000 agtcggttcc ctgccatcat cacgaaggcc gtgctagaca ccaaagtgaa gggcaggtac 6060 gcatatgttt cagcgggtat ggacgtcagt gcgaccatcg cggtctgtac agactcgagg 6120 catttccaaa gcgcatcggt tcggctgtac ggctcggtgg gcgaggctgt ttctgctggg 6180 ttcattggtc gcatgcccag ggcaggctcg taaagcctca ctggcaaggt cacataaagg 6240 caccaaacaa gagctgatag gaggagtcag gtcagctacg gtctcctggc ttggtgagag 6300 gacaccttac gattatcttt ggttatggca ggctcttgct tcggcagatc ctcaatctcc 6360 ttgcgtacgg atgagaagat tgaactgtaa tgctccttga agaacgggat gggcgaatcc 6420 tcgtctctgt cataaatcgg gaataaagac ctcagacgat cggtgatgtg ttgtatctga 6480 acaaaagcga gcaagctggg gtcggttgca aactcccttg cttctcggat cgctctgcag 6540 caacgcgcca tgtatgcgga ccacggtacc tgtggggctc ttcgcattga gatggccaca 6600 ctaaggatct ccgagttagc attgcctcca ggcgggcggt gttttgaagc aggggagcat 6660 acctagatgc caggaagtag cctcccagaa tcgctctttg ttcttctaac gtctgcttcg 6720 gggtctccaa atgctttcct ttggagataa cagcccatgc atctgacaac atggtcttgg 6780 gcatccaggc tagtggtcca gcaagagtgt taatgctcat atctgtaacc attccaatgg 6840 ccagctgcag caggccattg gcactcatac caagatggat atggatatca cccctagatt 6900 ccaagacaaa agtcagcaat tcaaattcag caagccttag aagagagcca cccaccaagc 6960 gtaattggcg atgtagatca gcaggccctg caacagctct agcctcctct ccctattgac 7020 gataaagtgt atggctacat gatgtctgat ccagtcatcg acggctttct gaactggggc 7080 tgtctgagga attagagcct gcataatgca ccagaacaga aacctcttct cagagtggag 7140 atcccgggct gtgacattca cgtggatagg gacgaaggga aagtggggaa ccatgcgggt 7200 gcggtagatg ttgaggtatt cctcggcttc gtccatggtg atggaaagcc cagggactag 7260 ttgcaaggta ttcaagggcc cagcatccgg tgtctgttgg gcgctgcgtt ggtggtcagg 7320 ggtgccagcg ttcgaggatg cttcggtagc aggttgaggc agtgagagcc cagcgcaagg 7380 cagtattccc cgcagcgcgt tctcttcata attcgatgtg ctggtggcag gagtaaggtc 7440 ttgagcttca tgttgcttgg catgttgttg agtctggaaa agattgacta ggccatcgat tttcttttcc agttcagtaa ctcggctgga agtagttgt cagctgaagc aaacctaatg 7620. caagtcacgg cattggatcc aaaaagaga gtagggacga gctctatgtc ggcacttgtg aaggacacga tgaagcacat aaacaagaca actcacctgg actttccccg tttctttctg ggtactggtt ccggcacccg acagcggatg ttcatccgggg aacatctagc ttgtgttagc 7740. tctctgcgag tcattcttgg attgcccaaa atggagtctg caatctctcc ctaccttgtg caatcggttg gttcaccatg ttccgtggcc ggtggccacc tgcatctgat cttgaggcgc 7860 gcacaattgc tgcaagctcg gagctttttc gcgttcgcga taaactccgg gctgctgccc 7920. tcttcttctg gagaaatatt tgtcatatgc ctaccagaaa gagagaccag aacagcagac atcacactgc ggccagtctc ccgccttctc caacgacttc agactcggat aagtctcatg 8040 acaccttgtt tattgagacg caaatctctg ctaccgatag gttgacaacg aagcaggcac 8100 tatccgcgtc aatcacagac cgtgaggtgc tcgtacctga catgttcaca tcaattctgt 8160 ctgtcgagcc tgtacagaac gctcactacg cccaagttaa gctagaagcg gatttgtgga 8220 tcgctaagta ctcacttctg acactcgata tgtaagattt atgagtgacc taacactcct 8280 aatctgtcaa gaaccctagg tctgaacgat gagcagaagt ctcgtcactc tcgcgcagac 8340 ttcgcctacc tggtgtcttg gtgggctcca agctgcgatg cagaagccct gcgtaccatg 8400 gttgactggc agaattgggt aagctctatc agcgtcgaaa gctttttttc tgaatataga 8460 atgaggcata cggtgggagt gggctaacaa ggtcttcatt atacggggcg caggcatttg 8520 tttgggatga ccgtaagtca catccttagc tttgttctga tgttatacga attcaatcta 8580 agttaaaaca tgcaactaag ctaacttgct gtttccgaca cagagttcga cgagggtcat 8640 ttgaaagaag accttcacgc tgcagcctct aacatcatca acatgacctc gattctggat 8700 gattgtcatc ctcttgtctg tcgtgaagac gatccaattg catatgcctg tcaagtcaac 8760 tggcgtagca ttcaaaaggt accttacttc tatctttcaa gctgcggcgg acagtagatc 8820 actaacaaag ttcccatagc gcgcatcgcc tggtacgcgg tgtcttcact atatccaatt 8880 gagagtggga tggaatgcta atgtaacagc ctgaagctct ccaacatcga tacaaagcct 8940 gtaagttgtg atcccaagcc tgctatgtca ccgcgtcatc gattgaccaa taaagacctc 9000 aaacactaca tgctcggagt acttggccaa gtcaactcgc gaaaattgga tgctcgaagt 9060 ctcactatcg aagaatttct agttttcga cgcgggacca ttggtgtcat gccttgcact 9120 gtgctggtcg agtaagacgc ctcgaaaaga tggccaatta tcgaatttgt caggaactga 9180 gcattgctga cctaagcgac ggcaggtatg cgctgggaat tgaagtccca gagcatatta 9240 ttaaccaccc atccatcaag gcttgccagg aagtagcggt ggacttggtt ctcctgttag 9300 tttatcattc aagtgacatt tacaccgagt gacagtacta accacaccgc tcctagtgat 9360 aacgacattc tctcatacaa gaaagacctg gtaggctcat ttcatatgat gatttaattc 9420 cgcagaactt gtacacgttc gctgataaac gtatgagaac gcattgttcc taacaatttc 9480 atagatcgag ggagaggact tgtctgtcat cggtatcttg cgctctcaag gttacactct 9540 acaagaagcc atggatgagg caggacgtat gattgatgtg agataccggc gatggtatga 9600 ggctttggct caaatgccga gctggggctc caaacaagat cgcttggtgc tcaagtacct 9660 tgggggcctt cgcgacattg ccctgggaag tcttttatgg aggtaagact ggccacaata 9720 cctacaacat acccccagct tccactttct ctgattcatg cattcttatt aacatttcta 9780 cctccgctgc cagcttttgg actggtcggt acttcagtaa acaagagggt gagatgctac 9840 gcgaaacccg tctgctgcgc cttccgattc agtagatggc tacccagcct cagtacaacc 9900 acacatcggc aatcccaact gtgtattaca accatatctc tctctatggc ctgctgtttc 9960 ttggattggg ctgctcgaca gaggctccga ctcgacctga gttagtgaga cctcggacta 10020 caagggactg ggacgaacgg aaggaaacca tcagagacct ttacatcacc cacaacatgt 10080 ccctcaaaga cgtcgtccga ataatgacgg agaagcatca tttctcggct acgtgagtag 10140 ctcactgcta ttcacttgaa aattcacaac taacaactcc acctacatgt agggagcggc 10200 aatacaaagt aaagtttgtt gcatggaatt ggaagaagta cactcggcga gataaagaaa 10260 tgaagcagtg gaacgtaggt tatgcagaga cgctccctga agcgctcatc ggcaaaagac 10320 ccattcgttg tcgaccatct atcgtctcaa aaacaactca aaatcgcgga cgcataagtc 10380 aaaagcaatc cccatcgggg cttcagtacc atgaagcatt tccaaataca ctgttatttg 10440 gttgcgaaga tgatcagcat gccagtgggc tatttttcgg cttgagagat ctcctctatg 10500 gcacttctcg tatcaatcct cgctggacac aagtgggagg ttcttggtgg ctacctgatg 10560 caggagagca tctcgtgtcc gtagctatcc tagctatacg ccgattcaac actggggagt 10620 tcaaattagg cggccatgca tttcggcagg ctttccgtgg tatcgaggat gtcattgaca 10680 aagacgcctt gacgattttc cagtcactgt tcctggagat accccttaga cttttggaag 10740 caggaaacat caaattaacg cagatgtaca ctgagtacct gtggcaaatg ctgcatgcga 10800 aaaagcggga agagccaatc acccaaatgg ccagaatgct gcatgccatc tgtgctagca 10860 cccccgaggc cctactgtac tacctccagc aaatgcatct catccttgca pricecttga ctcaatacg aggagacacc gactacaaca ccataaacgc acgcatgaga acgctccatc tgcagcaaaa tcacggatgt gatgtttcac agagctacct ggaatgcctc gacagctttg gggttttggc gcgcaactct accaccaagt ttagcgagct tgcttacgag atgagcctca tggaggttca tcggactaaa gccaacattg tgatggcgca cgcaccaata gcattgaagg aattgagcga taggtccctc accgagctga tggcaggcga tgatcactct gcgtttcttc agcaagtca aagtataagc gatctcgcgc aatacgcagt ggctacattc tttatccatg aatgtctgat acaatatgga gacttgaatg ggggcagctt atgcttgatg cgtatcatcg aggcacttga gcgtgcccag gacgtgtctt ccgacagcgt cattaattg tatcttgctg gacaattatt acggggttcgt ctgaaacttt cggatatcct ttttgcagcg gggagccctg aaatggcgga agcagtgatg aaagatgctc acatcatgaa gtacttgggg gagctcattt <h2 style=";text-align:left;direction:ltr">agatgggcgt tgagaatata ctcaaagaag aagatatcaa gatcttccag tcattgctat 11580<h2 style=";text-align:left;direction:ltr"> <h2 style=";text-align:left;direction:ltr"> tcgatattcc attcgacctc attacatcgg ccaggttcga agaacttcgc atttactcca 11640<h2 style=";text-align:left;direction:ltr"> <h2 style=";text-align:left;direction:ltr"> agtatctctt caaacttctt tcgtcccaga gaccaggact gccgattacc aggatggctg 11700<h2 style=";text-align:left;direction:ltr"> <h2 style=";text-align:left;direction:ltr"> gagcgatgta tcaactgagc cttggggact cggaagtgct tttccacaat atacagcgac 11760<h2 style=";text-align:left;direction:ltr"> <h2 style=";text-align:left;direction:ltr"> tgcatgattt aaatgctgac gagtaccggg atgcccgtgg gcacaacgac tttaacagca 11820<h2 style=";text-align:left;direction:ltr"> <h2 style=";text-align:left;direction:ltr"> tctgtgccag actcaaggca ctgacgatga aaggtgagca taagtctatg gatccgcaag 11880<h2 style=";text-align:left;direction:ltr"> <h2 style=";text-align:left;direction:ltr"> attacacaga cgccgttggc cggtttgacg ccttacgatt tgacgccata gctcaatgtg 11940<h2 style=";text-align:left;direction:ltr"> <h2 style=";text-align:left;direction:ltr"> gagagggttc cccccaggct tctggcatcg aattccgtcg tatccaagcc atttctcgat 12000<h2 style=";text-align:left;direction:ltr"> <h2 style=";text-align:left;direction:ltr"> tggatccaat gccagcagga ttcatgaccc tgtgcgaaag gtttcttgat gggttcgtgg 12060<h2 style=";text-align:left;direction:ltr"> <h2 style=";text-align:left;direction:ltr"> ttaaagatga acactccatg ttcatcttga ccacttgtga ccactggatt ctggaacagt 12120<h2 style=";text-align:left;direction:ltr"> <h2 style=";text-align:left;direction:ltr"> acgcagtcac taagtacttg atgcacgagt atctcatcca gtcgggatac attttgtcag 12180<h2 style=";text-align:left;direction:ltr"> <h2 style=";text-align:left;direction:ltr"> ctatccaatg cgttatagaa tctatggagg cgtttgagtg cgctgaggcg gtgtctttgg 12240 acgaggagat ggagatgtac catgccggga gagctttacg gactcgtact aagcttgcag agcttctgga agcaagtggt agactggagg agctcaaatgc tgttaccgca agcctttcag 12360. agtccagata cctgcagggt gtggaaagag acttggccga gctcaacaag agggatgcat gatcactatg tctcgagacg gatactttct ctgcggcact gactcttttt acatgcattc 12480 tgcagagctc gatgatcttc gtgtccgcag gagacagcat cagtcgtgcc gctgatgtga tctggcacgc ttcgatgcca atggtcttct ctgaagtctc tccgaattga gccgcagcct cttcgacaag gctcccgagt ctctcgacga tgagcgtgga ctcttgcggt gtgacagagt 12660 ttcctttcgc aagcaacagc tcgatggtgg ccaggagtgt agaagatcc ttcatccccc 12720. gaagctggct atacgtgtcc gcagtaatgc taaacagacg ctcaagagct ggggcaagtt gctctgcgtt gttcgagtga attgaataca gcagcttcgc caactgagag atcggatcct gccgcttctt gaggctcaac aactgggagg catagtgcag ccaagcccgc gtgacatcct tgtatttgcc gggaagatag aacgggatat atagcaaaac gacctgatat acctcaatag 12960 catctctttc aatgacattc tccaagtttc gaaaggcttc ccgtagtatc atacctccct 13020 gtttgtggtg cttgttctca aacagacgga ttgcttggtc tatctgtgac actaggagcc 13080 tcccggtagt cggaagcccg gtaaatctga gttttccgct cgaccaacct gcgtcatcgc 13140 gtcggaacgt gccatgaata agatctcgaa ggctgatgta gagttgcaga gtccttttgt 13200 catttccgtt ggcgcaggat aacttggttg ggggcatggc gcttgaggtc ggtactcttg 13260 ccgaactgtc tatcacatgg cgtcttctga tagcccaagc ggactctatg ctgatgttcc 13320 tcttattccg catcggccgt ctgcacgtcg tctgaccctg tgacatctca ataaaccggc 13380 cagtttcttt gccttcctgg accgattggg tgttgtactt cttccattcc catcttttga 13440 actgccgctt atacatcctt ttactaaagt gcgacgtcag ccttagccgt cgctggcgtc 13500 cggtgataat tcttacgtgg ctgcaaaaga gtggttccta agcatggtgt ttgcaacctc 13560 ctcaagggtg aagttctcaa ccaggtaaag ctgcctaacg gtgtctttca tggcgtccca 13620 ctcttgccgt gtctgtggac gccgtggctc agctttaccg gaggactcgg gcgattctgg 13680 gggccgaagg ctgagggtcg gcgtaagacg ttgatgtggc tttggggcca acagcttgta 13740 gctggatccg gctttagagc catgctgaga aactgcttgt gcggggtttt ccggcttgtc 13800 atagtttcta tcaatgatca tatgtcatac cctcagttga cgccggcgcg accggtgccc 13860 ggcgcctttt tcaacacgcc agcagcttca cgatttgcag gcaacgacaa cgagcccgtc 13920 agacggcgcc tgttcttgga cggtgacgga gcccagggaa gcacaacaac tggaaccagc 13980 ggcgccctgg taggaggttc gagcttcgcc cctgcgacgg tttcttcggt cggtacgagt 14040 ggcggactgg tctcgacgtc gcaagctccc cagcagaacc tcccgccggt cacaagagct 14100 gcacaggcgg tcaaccaatt cctgcaggcc gatgagaact acccggaagt ggattcatac 14160 tgtaggcgta tgtcatctct ccctaagccg ccctttcccc cctcgatggc atatctgatt 14220 cccaaacagc aggcgcatcc tccgagtatg actacagctc gctcgattcg gcgtgggccc 14280 ccttccacaa gacgcaaatg taccccatcc cgaatcaggt cttcagccat tacaacaccg gccagttgca gacgttgatg ggcttgttcg cggagatcaa ccacgcgtgg gttgttatcg 14400 14460. acaactcgct gttcctctgg gactacacac accctgaccc tgagcttatc ggcttcgagg accagcctca cacgatccac gcagtcgccc tggtgtcccc gaagcccggc atcttcgtca actccatcac ccacattctc gtcgtcgcaa cttcgtccga gatgatcctc ctcggtcttt ctgccgaacc gaccccctcc ggacggaga cggtttcgct ctacagtacg aagttgaacc tccccctgcg aggcaccgat gtccgagtca ttgctggcac cgcggacggt cgcatcttct 14700. tcggcggaag caacgatacc gatattcacg agctgtacta ccagcaggag gagaagtggt 14820. tctcgaacag atgcggcaag atcaaccaca ccaaccccgg ttggtcctcg gtggtcaccc ttcaaagtgg cttctggcag cacaagtctc cggagggtct tcagagcatc gtcattgacg actcgcgga actggtctac actctgtcta ccaggtctac cattcgaca taccacatgg acgggcctaa caagctcacc aaggtcatcg aaaaggagag aaacgcatgt cttcgtgaca ttacacacat gatcactcag tcgcccttgc tcaacgagcg cacgacgatc gtctcgatta 15060 gtgctatccc ctctcgagag gcctcgaagt tgcatcttat ggcactcacc aacaccgggt 15120 gccgtctgtt tcttagcgca acaagctctg cttcctacat gattggtggc gtgtcttcta 15180 ataccccccc tcagagcatg caggttcagt atatcaagtt ccctccggcc gaaaaccctg 15240 cccgcgccaa tacgaatact gccggcgaga ccattatcga tttctcctca aaagccctgg 15300 cgatgagcag actcggtgct cgttttgccc caggctactt cctggatttc gtagttaagg 15360 agtccgaccc caacaacgac cagcttttcg tttctgcccc cgaaactggc cgcatcaaga 15420 ccaatgcatc atctcaacaa ctcaagtact acgaacacgg caactggatt gatattggaa 15480 gtagagctga agatgttggt ttggtcacga agccttttgc tgccgcgaac caacccattg 15540 gttttggaaa tgagcttgct gttcagttcg atgagccttc gaccgagttc gccatcctca 15600 ctaataccgg tgtgcacatc atccgccgcc gccgactggt cgacactttt gcttctgcta 15660 ttcgcggagc cgttggagac gagggacttg aactggagac gcggaagttc attcagcgat 15720 atggccgggt ggagacgatc tcctgtgctc tggcggtagc ttgcggacat ggtggtgatg 15780 cacgacctgg agctgctcgc gccattgacc aggccacgga agatcgcgct cgcaccgttt 15840 tcgtcgacta cggtggacag ccctcaatca ccgagaccga cggcacacag ctcaacacca 15900 cctctgtccg tctctcccct agacacgacg ccctttgcgt atacatcact cgcctgattc 15960 gaaccctctg gaagaaccat gtggttgccc ctgctgtaag cccgaccgga ggcgtcacaa 16020 ttggatcaag agtgcctctt gccaagcttt tgtcggttca ggagcagctc gagcgccttc 16080 gcagattcct cgaggccaac aagagcttca tccaaggcct gtctggcccc tctgatctcc 16140 aaagagtggc gagcaagcaa gaggaggttg ctctccaggc tgagcaccag gccctgcacg 16200 cgctccagaa gctgatggag agcatctcag aaggcatctc ttcgtttca atgttgttcg 16260 atgagcgtgt cagcgacatc ttcacaagac ttgacgccca agcgcaacaa gaactgaagg 16320 agctcacata cgagaagttg tttcgcagg ctcctggaaa ggagctagcc aagttgctcg 16380 tcaaatccat tgtcaaccgc aatatcgaga gcggctccaa cgtggagact gtggctgatg 16440 ctctgcgtag gagatgtggc agtttctgca gccctgatga tgttgtcatt ttcaaggccc 16500 aagagcagtt gaagagggca tccgagcagc ctctgaacag caacccgtca cgcacactgc 16560 tgcatgagag tttgagactt tttgagcgcg tcgccaacgc cctttccttc gccaaccttc 16620 acaacgctgt ggctcagtac attgagctga agtactacgc aggcgcgatt cagctgtgtc 16680 tggtcgtcgc tcgcgagaag gacagaggga acagtgccct ttcctgggtc cacgatggca 16740 agcctccgaa tgaccctcgt gccaatgcct tctcggaacg tagaaagtgc tacgagttga 16800 ttcacgaggc tctgcaacac ttggatgccg cttcaagcag ggaaccggag acgattgacg 16860 gcagattgac gctgattggc accaagcgcc tggaggcata cagtgtggtg aacgattcgg 16920 acgatgaggt cttccacttt gacctctacg agtggtacat tgaacagggt tggaccgaca 16980 gaatcctggc tatcgagtca cctcacgtta tcacatacct ccagagcctc gccaggtcta 17040 atgttggaca cgccgagctt ctctgcaagt tctataccca gcgtgagaga tatttcgagg 17100 cagcccaggt ccaggccgac ctcgccaact ctgactttga cctcggcatc aaggagcgca 17160 ttgtgcttct cagcaaagcc aagggcaacg ccagcgtcgc caccgccggc atcggcaggc 17220 aacaacagca ggtcctcgcc catgaggtca gcgagcttct cgacatcgcc aacatccagg 17280 acgatttgct ggaaaggctc aaggccgata gccgtattgc accggagcgt aaggcggaga 17340 ttcaagaggc tctcgacggt cagatactgt cgctgtcaga tgtaagtcac ttccctatgt 17400 gctcacgaca aagctaattc gcttcagctc ttcaaccaat acgccgacca agcaaactac 17460 tacgaccttt gtcttttgat ctaccacagc gccgactacc gtaaccctag cacaatctcc 17520 caaacatggg tcagcctgat tgagcaaatt caccaagagg ccgtcgaccg catggaggag 17580 gccggccccg gcatccctgt cctgccgtcg ccgtacgagt ctgtgtctgt caaggtccag 17640 aacatcgctc accgcacatc actcgacagc ttcatctttc ctgtgccagt cctcctcccc 17700 gaagtctgcc gctacgccgt tgagtaccac caggatgctg agattggtgc ggaccccaca 17760 tggcccgtcc agctcttcat cagcttgggc gtctcccacg acatggttac acgcgtcctc 17820 gagagcgtct tcgacacaca agactatggc ttcagcggca acaaccgtaa ccgcatcatc 17880 gagcttattg tcttcgcggt cgatgtctgg caacgcgagg tgaagcgtcg cggcggcgcc 17940 tctctgaagg gtgacggtgc catcggtgca tgggttaagg atctgctcag caggtgcgaa 18000 tcgtccttgc cgcctgccgg ccacggctcg aaccctggcg gtgcggactt ggcggaggtg 18060 cgcagacgtc tgcgtgctgt gaggagggaa gttgagggtc tcgtggagag gggaccgtca 18120 ggaagcttgc gcttcatgta aatgcaggga atcacggatt atatcagcca gctgccgcag 18180 ccggtccagc tcgtcctggc cagcgttggc gctctctttc tggccaacaa ggccctcgcc 18240 tatgtcaagt tcctgctcgg tgtcttcgtc ctcagcggta gcaacgtacg aacccccctc 18300 tcccctcttc tacttccagg gcgttactga caacagcttg aacagctccg caagtatggc 18360 aagcccggca cctgggcagt cgtgaccggc gcctccgacg gcctgggcaa ggaatacgca 18420 taccagcttg ccagcaaggg cttcaacctc gtcctcgtct cccgaacaca atccaagctc 18480 gagaccctcg cccttgagct cgaggccaag ttctccggca aggtccaggt caaggttctc 18540 gccatggact acgcccgcga cgacagtgcc gactacgacc gccttgccca gctcatcgct 18600 ggcctcgacg tcggcatcct cgtgaacaac gtcggccagt cccacagcat ccccgtcccc 18660 ttcgtcgaga ccgcccgcga ggagctccag aacatcgtca ccatcaactg ccttggcacc 18720 ctcaagacca cccaggtcgt cgctcccatc atgcaaaagc gcaagtccgg cctcatcctc 18780 accatgggct ccttcgccgg ctggatcccc acgccctacc tggctacgta ctccggttcc 18840 aaggccttcc tgcagcactg gagcagctct ctcgctaccg agctcaagtc ggacggcatc 18900 gacgtgcagc ttgtcgtcag ctatctcgtg acgaccgcca tgagcaaggt ccgcaagacg 18960 agcgttgcga tccccaaccc caagaacttt gtccgtgcgg cgctcggcaa ggtcggcact 19020 ggcatctacc agaactttgc gtacacctat acgccgtggt ggacgcatgc tctgatgctc 19080 tggattgttg agaacaccat tggtgttgct cacgaatggg ctctttccgt caacatgaag 19140 atgcacgctg ataccgcac ccgtgctctg aggaaggccg cccgcgaggc caagaaggcc 19200 taattaaggg gcctcgacgt agaagctgcg aacctggggc atccagatca gggcaacggt 19260 ggccagcgtg aaggggaacg cttgaggaac gccaaaagtc atgctcttga agacgaagag 19320 ctcgctcgca aagtggccca gcgcaatgac atatgtccag atggcgaggt cgtacatgtg 19380 cgcgtagtgc aggtggtagg cgcagtacag gcggacgacg cttgtcaaca gagtccaggt 19440 gccgaagcag cgggcggcga gcggtgtgcc ctggtccagg gtctcggact cctttgcgcc 19500 ggcgggcgg gggacgaact tgttgaccga gtcctcgggg ttgtactttt cggagcgagg 19560 agggagggta gggttcttga cgaatttgcc attgtagacg cggcgcgagt agtggagggt 19620 caagtagttc tgggccgagt tgccaatggc aaggagggaa agctgttggt ggcactgggt 19680 tagcttcgcg atcttgcgcg tggtgtata accagtgaca cgagacttac gaagagcatg 19740 tagtagggca ggataccctt cgcaggagga agcaagtcgg caagagacac cat 19793 <210> 4 <211> 20406 <212> DNA <213> Artificial Sequence <400> 4 atggagtacg gcatcctcct tctgggcttc cgccctttct atcttttggg cgtctgctcg 60 cttctctctt tcttgttcgt atggattttc gcgaagaaac cttccgagtt ggatgcgcca 120 cttttgacca acgactctgg agacttcttg caaatcatga aaaaaggcta tgcagaggtg 180 agtggttcat atgtcggatt ctcaaccgaa tagagtttta catcagggct ttgctgacac 240 ttgtcctagt accgcaatcg cctattcaag atcccgacat ctaataagcc gatggtaata 300 gtgccgactc acttactgga cgatctcaag gcgtaccctg aggacaccat cagttttcgt 360 cgggaaatgt acgaccggta cttggggcag tacacatccg tcgcttctaa cagcagtgcc 420 atgatccact cagtcaagtt tgatctgacc aaaaacattg ggaatctcat tcccctgatg 480 caggaggaaa tcagctacgc tatggaccat ttcatgtcgg ggtacacgcc tgcagaagga 540 tggaccaggg taccggttta cgccttgtcc acgcgtgtta ttgcgatggt gtctggcagg 600 gtgtttgttg gcttgccact gtcgcggaat gaggaatggt gagtatacct taatcttata 660 gagaacgatg taccttacct gatgctttga caggattcag accgcgattc attcaaccat cgactctttt gttggagcgg ctatgatgtg gcagtacccc tcatggtctt ggcccatcat 780 gcaatggatc gtaccccaga caaaccgggt gcgatcctac cgtcggaggg ttgcagagat 840 gctcgagcca attattgcag gtaggctcga cgatatgag aaaggatccg accggaaac gccttcggat atgattcagt ggctgattga caactccaat ggccgtggcg gtgacctcgc attccaggcc aacgagcaca tcgtgatgaa cgtcgccgcc atccacacaa ccggtggtca gcttgcacat accctctatg atcttgcgcg acatcctgag tacttacctc agcttcggga 1080 agagatcgat tcggttttgt cccaggacgg cagcgttacc aagcagggcc tcttccgcct 1140 gaaaaagatg gatagcttcc tacgtgaagt gcaacggctg agccccccat ccatggtttc cacaaaccga aaagccctga agactttga actctccaac ggagtgatca ttcccgctgg cacatcgctg gctgcttctc cggcatgcgt cagcagggat cctgctatct ggaagaatcc agatgagttc gatgggcttc gattcttcaa actccgcgac tcagagggag aggagaagta 1380. ccaatttgca actgtcaatg acgacgcttt aagctttggt aagtgatagt cgatccagga 1440 tgggtgaatt tagatgacgc taatattggt ccaggtcatg ggcgtcacgc ttgtccagga 1500 cgctttttcg cctctgctga gctgaaggtc gtcttgatcc acattctccg aaactatgat 1560 atcggcttat gtaccgacta tagcaaggat cgacctctga accgctttgt agaggttatg 1620 gctgcgcagg atgtcacgta tgagcttcgt tttcgcaggc gagaatagat ggcccctcac 1680 gctgagattg acgtcgacca tgtggtccga ggatcggagg cgaatagtca agatgcagac 1740 ctagtcacga tctcgatcgg caaaataatg gactgcgata aatcggccga ggagagtctg 1800 ctgaatgcct cgaaagatct gggatttttc taccttgacg tgagagatca tccttctcgt 1860 gaggtgacga accagatcga gattgccaca tccgcagcgc tgcaattcta cgacctgcct 1920 caaagcgaga agtccatctg ggaggtcaac aaagaccata tctccgggga ggagattatc 1980 ggcgggtgag aaaacataga acgaaacgta agccttgatt aattcaagag ggctaacgca 2040 actgatgcat agatacaagc ctgccggaat tcagactggg tctgtagcag gcaaaggtga 2100 tggatttgaa ggcttcctgg taagttctac tgctatcttc cggaggcgac gtcaccgtgg 2160 tatcctaacc taacctttgc tgcagatttt cgagcacgcc ttgatgaatg ctgctcgtga 2220 caagcccgcg aatctcccga aggcattcca ggagagacgt gaggtgtttg aagctacgaa 2280 tcagacactg ggagatattg gaagggttct tctggacagc ctgtcgcgat ccctcgatct 2340 gaacggcaag cagaaccttg ccgagcacca ccgtaaaggc catgcctctc ctaccgcgct 2400 cggacttctc aagtacctac cttacgaacc acaaagcgag gatgtcggcc atgtctccca 2460 cactgacatt ggaagtcttt ccatggtgtt ctccgatgtg ggcgggcttc aggtgtatca 2520 cccaggaaag aggtcttgga tgttcatccc acccaagcca gggcataccg tgtgcaacat 2580 tggtgattca gccgagttct tgtccaagaa tgtgctgcgg tcgtctctgc atcgcgtcat 2640 cccacaccca gcggagaaag gcaagagcaa gctgaccgtg gtatatctaa tgagacctga 2700 gacagatacg gtcttcgttg accgcgaggg caaggagtgg agaagtgtgg actggcacaa 2760 catgaagaat cgtttgtttg cggaggactt ggagtctcag gctagcaacc tcgccctgac 2820 tggccgtctg ggccacgacg atctttggga cgagaaggat gtctgattac tgggctgctg 2880 tagactggat gacgactcgg acccccttag ttccgtcttt cggtgttggg acgaacctgt 2940 catagtgtat ctcgacgtct tccctgcccg ttccatagag ggccatgctg gagcccatac 3000 ggcggatgag cagcccaaat accatgtaaa gctcagcgta cgcgaggctg catgaatatg 3060 tcagcgattt gggtgtcagt cccgatgcaa aagcacgtaa aacttacttg attccgatac 3120 actgtcggct gccacgagaa aatgacagaa catatttgtc aagatgatga tgacgccgcc 3180 cttgctcatc cagccagcgc tctggcctaa attgctcagg atttgggaat aactccggat 3240 ggttgtgaat aagagtcgcg gtcattccca cgggtgttcc tgctggtatc tcgtagtcgg 3300 ccatgacgcg tttctcgccc ttctcaagag agagctcgga cttcaaaaca aggggcccga 3360 ccggattgat gcgctgtagc cgcgtgctta caccgtagga aaggcgcagc ccctcgcaga 3420 taacggcgct gagatacggt aattgttcca aacggttcca gctgactctc tcatcggcat 3480 cgggaatggc ctcctccaac tcatctctca gctttctaag aatctcggag tcatcgagga 3540 tgtagaacag catcgctgaa agtgcccatg ctgtggtctc ggtcccggca ccgattacga 3600 tctggccctc ttgccacaga cgctccaatc tcttctcctg ctctgggagg tcgcctgtca 3660 gtagctcatg gaatattgtt ctcccagact gatcgtcgat cttcccaaaa gcaatgtcat 3720 tttgagtctg tcgaatctga cgtctgatat cttcctgaaa ccgcacgtaa atcgcggctt 3780 caggattgat ccgcatgacg agactctgag gaagtctgtt catcagggta atgagccacg 3840 ggaactgctt aaaccatgga cccatgtccg ttccggcgat aatggcgcgg tgaaagtttg 3900 gctcaaaagt tgaagatccc aaaaagttgt aactcttcgc aaaggcatac tccgtgacaa 3960 cgtctgtcgt tacgcagctg aatgccatgt tgatagccac gggctttcca ctcccagcgt 4020 atgtccggag ctgcgagacc agcttttcaa tagcattatg aatgactggc tccagtttat 4080 tgaccgatga tttggagaag aagcggttga gggcaccgcg tcggaggcga tggaggtcat 4140 ggctgggtgt tccgaataca ctgtccggga ttccgaactg agcgctgaaa tacgggtatt 4200 tgtctcgctt ttttccactc ccggcgtaaa gctcgtcaat gaaggagggt tcattgacat 4260 ggagctcgtc agggctgata cggacgatgg gaccgtactt ctcatgcatc cgttggatct 4320 caaacgtata acgtcctcgc ttgataccgt cgaagtagaa ctcgtaaaaa accgtaagtg 4380 ctgcgatttt cggaccggga aactttgcca gcgggtgaag gtacagccta tagatagcaa 4440 gtccgatgag gtacaacgcc aatacaacgg ccgcggttaa gatgggatac atatggctgt 4500 tacggacgcg acagaccttt gtcgaaccga caacgacaat aacgaacctc tgctgaagac 4560 cagcgttctt gacctcttcg atcagcatgc gaggcgcagt ccgaatagca ccgccgcaga 4620 gttccagggt cagaccatca cttacgatgg attgtacaat gcttccatcc aagtcgcttt 4680 ggaattgcgt aaacgaggat tccgtcctcg cgatcgcgtt cccctgatta ccagcatggg 4740 cttggagatg gtcgcctcgg tcttgggaat tctgcggcta ggagcttctt actgtcccat 4800 agactttcaa gcttggagct tagaacgagt tgttgccact ttggaagccg tgggctcacg 4860 cctagttttc agcaccgtgg acacaacgat aactggctac gagctggttc gtgtgcctga 4920 aatgctgagg aagaacaacc cggtgaccga cgaagcaaca caacaactaa aactgatcag 4980 gcaagatatc aggaaatctg atctcattta catcatcttc acttctggca ctacgggaaa 5040 gccgaaggga gtgatggtcc cgcacagcag tgctgcccac cttgtgcagc aaagcttccc 5100 cggagcaatg agaggaagcc caggggagaa ggttttgcta ttcttctcag ttgcctttga 5160 tggtcagtca cttttctatt ctttaatcgg aggtctctca gcagcgaact gtttagtcta 5220 acacttgcca ctcctacagg atgcgcagga gtcatctttt cgacaatttg ccatggtgga 5280 actcttgcga tggctcaacc gtccgacgtc atcaacacag cctcttcatg cagcactctc 5340 attctgacac cttcaatgct ggccagtctg gaacccaccc ctgagttcga ccgtgtgcac 5400 agcatttaca tgggaggtga agctcccact gaagctctgg tcagactttg gaccagtccg 5460 acaagaaagg tttacaattg ctacggaccc actgagtgca ccacggccgt ctctaccgta 5520 gagatggttc ctgatggccc gatcgttctc ggccatctgg tctcggaggt ggagctggtg 5580 ctgctcgatg agaatctaga acatgaagtt ccgcagggag agatctgcat cagagggcca 5640 tgcttggcgg ttgggtacct caatatgggg gctctcacac aggaaaagtt cttcttcaga 5700 gacggcatcc gacattaccg tacgggagac cttgcccgcc gtgacgagga aggtctgcat 5760 tttgtggctc gggttgaccg catagtcaaa aatcgcggat ttctgatcaa cttggaagcg 5820 gaagtcgaac cggctcttcg cagcttttca ggagtccaga aagccgcggc attccttctg 5880 cagaagcagc tggtggcctt cgtgacgcct tctgaggcac cggtcgagac actgcgcggg 5940 cacctcgcta aacgattcga tgcttttatc atacccgacc tcattttcgc cgttgatgac 6000 ttcccgttga ccacaaacgg caaggtcgat acaagagcct tgcaggcaat ggccacgcat 6060 caagttgatg aacacctacg cagttccgga gacagtgagg ccgacaaatc tcctgccttg 6120 gacgttgttc tggaagcctt tgcagatatc ttcaaccgac cagcgagcca gataccgccc 6180 acagcgtcgt tcagactcct cggcggcaac tcccttacgg ctgtgaaact tcagtctcat 6240 cttcgccgcg caaaccttag tttgatgttg agcaagatgt tcgaattgga tacggcagca 6300 gcgatagctc gggcggcagt ctcaatggac tacaacattg accgtgtcag ctcgaccaac 6360 gtgcatccat caactacgcc acttacgccg catcaaatcg agcttttgag ggagaccgag 6420 attgactcat cgagcaatta caacttctac tgcctcacta gaatcatcgg tcacagcccc 6480 gtcaatactg aacgcttgcg agaggcatgg gaggtgatat tcgcccgtca ctccatatac 6540 cgaaccacat tcgacatgga cacgaaaacc cagacagtgc acgccgggcg agacatcaac 6600 tggcgggagt tcacggcgcc agactatgaa cagatgaagg ccgtttctga gcgggagaga 6660 gaggtgttgt ggacaatact acgttcccaa aagccacgga tacccattct gaagcctcat 6720 ttctggatcg ttgaagtgcc tggagaggcc attcagatga attggcttgt tcaccacgtc 6780 tatactgatg cgtggtcttt cgcaattctg ttggaagaac tcgaacttat catcaatggc 6840 aatgctgcca cacttcaacc tgcacctggc ttcgaactgg tagcccagca cttggacaaa 6900 gaagctcggt tacaagcaac agctatacgg gatttctgga gtaggtacgc gacgccgtgg 6960 acagagctga gaccgattca actacccgtc cccagggagc ccagtatcca gccatgggac 7020 aagtgggagt ccgatatccc tttaaaagtg gagagccttg aactgtttgc ccgccagcat 7080 gcagtctctt cggcgacagt gatctacact gcgtgggcct tagtcctcac tgattacacc 7140 ggttctggta ctgtgggcat gaaggtcagt gtctccggga gaaatatcga ccatccggct 7200 gtcgagcgcg tcatgggatc tctcaatggc cgttgtccgc tcctagtacc gaccagtaaa 7260 gacgcaacga ttggggaaac actgagatcg ctgcagagta cctttctccg aatcaatgac 7320 tatcagtggt cgtacccgga gttgaaaaga catgtagccg aggtatttgg agaccaagcc 7380 tactggtttg attcccaagt agttgtgtta ttagatacgc ccgtagacac aagtcattgg 7440 ggcattttcg aggtgcagaa gccgactgct cccatctggc ttggtgtcat tcaaggcggg 7500 gacgtgttca acatgcgttt gcgatacgat ggcagttgtt atgcacagga tagtgttcag 7560 agcatgggca ggagattcat cgacagactg atccagttgg ttgattctcc cctcgacagg 7620 agaatcgggg atctaggtca catcacgcaa gactgaatgg ccgtcgtcac tgtgtcagac 7680 cctacttttc gggcgtacaa aggcactcaa gccaagcgat acgacgaagg ccgccccgcg 7740 tacacaccgg cgctttacga ggccatactg aaatatcatg atgataatgg cggccaccgg 7800 ggcacagtca tcgacgttgg gtgcggccct ggaagagcaa cgcgggaatt ggcacgtttc 7860 ttcgacaata cgatcggcgt tgaccccagt gaaaatatga ttgacaccgc caagcaagtt 7920 ggtggtgccg ggaacactgg caagccgatt gagtactacg tttctcccgc tgagaaaatt 7980 gcggacctgg aagaattgcc agatggcaaa gtcgatctct tgacagccgc cgccgcggtt 8040 agtgagccct gtaatttcaa acccatgccc tgggaattgc taactctccg aacaggcgca 8100 ttggtttgac atgcccaagt tctggggcca agctgcccgt ctcttgcgac caggagggtc 8160 agtagctcta tggaccactg gctgtttcta tagtcgtatg tgcctcaata ggagaaaatc 8220 ccgcttcagg gcatcaatga aaagatcgat ggctaactgg atgtattctg agcagaccca 8280 gcgtctgaca acgcaggggga attaaatcga attatgcgcc agctggaaac tgacgagctc 8340 gagcaatttg agctgacccc taacgtgctg tctcgcaccc tttacgacga cctagctctt 8400 ccatggaaac ttgagtctcc tcagcccgac tttgaggagt cggcctatgt taggcgagag 8460 tgggatcgcg acggtgttct taccgacggg gaggactttt ttggagactc gctggaaatc 8520 tcgttagaca tgttggagaa gggttacgac acggctagca ctgtcacacg ctggcgggca 8580 gctaacccgg agctagctta tggccctcat gattgtgtca agaggaccat tgcacagctc 8640 cgtaaggccg tgggacctga atcgaacgga ttgattcggg gaagtacttc gacagttctt 8700 ctgctcttta agcgtcgcta aatgcaccag cgcatcgctg ccggcagcac cgtagacgca 8760 gcacacaccc caccacagga aaagtcattt gccgaggaat ctgaagcgtt tcttgaccaa 8820 gatgagaagg ctgtactaca gagacagatc gctagtccga cctcgtggca caattcagtt 8880 cgctttttgt tgagttgcac cagcagtctg gatggaatca ttcttagtct aagcgcaatt 8940 gctgccatca tagggggggc cgcaaaccct tttgcgatgg tgagtctcgc cttaatgaag 9000 tttaaatgtt gaagtcgact gataaagaga cattcgtagc ttcttctggg aaacataggc 9060 caatcattcc gaggcttctt tgttggcgaa accgctttgg aggatttcaa ccacgaggtc 9120 aatcaactca gtcttttcta cgtctattta gcgattcttg aatttgcggc aatctatggt 9180 gccactgttg ggttcaccgt agcaggagaa cgtattgcac aacgtatccg cgagagatac 9240 ctcgcagcca ttctgcgcca aaacattgcc tatttcgatg gccttgcagt cggcgaagta 9300 aacgctcgcc tcaacaaga tatcagcctc attcaggatg ctatcacggg caaagcgtca 9360 gccacgctct ctgcaatcgc cacattcgta tcggccttga tcatcagctt catcaagagt 9420 tggattctga cccttgtttt gtttcctgct cagattctca ttgtcggcac catgagtgta 9480 ggcgcaaagt tcatgatcaa gtacacggtg cagtcaatcg gagcatacgc tccaggagcg 9540 agcatagcag aagaggctat cggatctgtg cgggccgtga cggcgtacgg tatgcagcag 9600 gccctggtcc gccgttacga agagcacgtt gagaaggctc gcaaagctgg ttccaagagt 9660 ggcatcgctc tggcttgtat gattgcggcg atgaacggca ttatcttctg gagttacggc 9720 cttgcgttct ggcagggttc acgcttctc gtacaaggaa gggtctcgct ttcggccatc 9780 ctgacgatcc tcttcgccaa tatcacggga gcgttcgcgc tgggcaatat cagtcctcac 9840 actcaagcat tcgtcagcgg tatcacggcg acggacaaaa tttcgcaagc atgctgtcgt 9900 cagtcgccaa tcgactccag cgcctctacg agaacaaacc cgcaaacgat cacaggggca 9960 attgaactgc gaaacgtccg ccatgtgtat ccctcacggc aagaaagtct agttttggat 10020 aatttcagtc acttcttccc accaggaaag atgactgcta tcgtaggctc atcaggctgt 10080 ggcaagagta caattgttgg gcttcttgag aggttctacc aacccgtcag cggcaatatt 10140 tgtaagtaca cctgtttcgt cttgaaatgc cgctgctaat cgactcccag acttggatgg 10200 tgtcgacatc tgttcgattg atgtctcatg gctgcgtcag cagatcggcc ttattggcca 10260 agaccccacg ctcttctcta ccaccatctt cgacaacatc agtttcgggc ttctcggctc 10320 ctttcatgaa agcctaaatg ctcagctagt agaaaagatg gtcgagagtg ccgcaagaat 10380 agctgatgcc tacgacttca tcatggcttt gccagacggc ttctcaacta atgttggcga 10440 ctccgggtca ctgctctccg ggggccagaa acagcgtatc gccattgcaa gggccattat 10500 cggaaacccg aggatactga tattagatga agccacatcg gctctcgacg ccgttgcgga 10560 gcgacacgtt cagcaggctc tgcagacagc gtccgagggc cgaaccacta ttgccatcgc 10620 ccacaggtaa gaagactcgc gatgaaatta tttgaattga aaactcagta ctaattaggg 10680 catagattat ccacaatatc tagagctgac aatatcatcg ttatgtgcaa gggtcgtatt 10740 gttgaacaag cacacacgc ggagttgatg gaactgaggg caatttactt tgacatggtc 10800 caacagcaga gtgtccaaga aaccactcca accatggcaa tttcggatgg aatttccact 10860 caaaacgagt tcggcgagaa cgccgttcca ggcaaactgc tcatggagtt tgacgcaaag 10920 cccacacaaa ccactgccac gacgccgcaa ggtccacgga actcggaacc atgctcgact 10980 tggaatttgg cactctttgt catagagtctg aaccgcaaag acggtcttct gattatcctt 11040 ggtcttgtat tttccgtgat tgccggagcg agtcatcctg tgtaggtcac cccatctaaa 11100 aacagggtag ccagccctac ttgacaggat tcatgttgac ttgaacctca ggcagtctgt 11160 gttccttgcg aagatcatcg ccgcaatgtc tctgaaatct tcagattacg gactcttcg 11220 tgatcaggtg aacttctgga gctggatgta tctgatgatt ggcttcacca cggtcttcgg 11280 ctggctaggc cagggagtct gtttcgcctt ctattctcaa cgcctcacgt acaatgcacg 11340 tgtgaaaggt ctagagacca tgttatacca cgacatcgtt acattcttgc gtgacgatca 11400 ttccaccgct gctctcacca gtgttctctc cagcagcgca gccagcctcc aaggtctatc 11460 gggtgctgtt ctcggaacgc ttctcgttgt tctcacgact ctcattgccg gcttcgttct 11520 ggcaacggct atcgggtgga agctcgccct tgtctgtgcg accacaaccc cgatccagct 11580 tgcatgtggc atactgcgct tgaagtgcgt cgcgctgctc gaaggccact cacgtcgggt 11640 gtacgagtcc tccgcgacgt atgcctgcga gtacagctcc aatatccgta ccgttgcggc 11700 gcttaatctg gaggccaaga ttcagagaga ctatcgccag cttcttgagg agcaacgaaa 11760 gaagtcatta gtgtcaatca gtcaatcctc gctgctgtac gctgcgtcgc agtccctgaa 11820 cttcctgtgc gcgtccctgg cgttttggta cggcagtagg ctagtggcaa ctgagaacta 11880 cacactcttc cagttctttg tctgctatac tgctgttatt gctggcgcgt actctgctgg 11940 tgccatcttc tcatttgctc ccgatgtcgg caaagcgaga gactctgcgg agcggatgca 12000 ggcgttgttt cgacagcctg tcagcatcga tgcaagggca agtgatgggt ctgttccccc 12060 aagcaccgaa ggatttatcg agctccggaa cgtgactttc cgttatccaa gccgtccgga 12120 ccggacagtc ctcgaaaata ttaatattgt cgcccagcct gggcagtata ttgctttggt 12180 aggaggcagc ggtagcggca aggatacagt catacactc cttgaacgct tcttcgatcc 12240 cgaccatggt caagtcaat ttggcgaga aaatatcaa gatttgaact tgaggagta 12300 ccgaagtcag ctggctttag tcagtcaatc gccacattg tcgacggta tgatacgtga 12360 caatattgta tttgggtgg aggaccagag tgttgcggag gaagctgtca tacaggcatg 12420 caaagacgct atattacg acttcatatc ttctctcccg taagtaccc tccgtgctga 12480 atatcggatt cccatgcact aaacaaattt tccatttag agatggattc gatacaatcg 12540 ttggggcgg gggtgtcatg ctctcaggcg gccagaaaca gagaatcaca atcgcacgag 12600 cgttgcttcg aaagccaaaa gtcttactc tcgatgaggc aacgtcggcg ctagactcag 12660 agtctgagaa gtggttcaa gaggcgttag atgcggctgc ccgtggccga acacgatcg 12720 ctgtggcgca tcgcatcagc acagtgaaac acgccgattg tatttatgt gagtttacca 12780 gcatccagaa agaaacgttc agcactttcc tggttttggg ctttctaact gctgccagtg 12840 ctcgaccgtg gccgggttgt tgagcaggga acccatcgag ccttgataga ggcgaagggt 12900 aggtacagcg agctggttaa aatgcaaagt ttcagtgtat agaacgcaca agaagcgaaa 12960 agcttgctat tccccctctt tctgcatttg taccttctct ctcgtcttgg atggtagctc 13020 agatcttctc aaagtatgaa tgattgacca aatagtgtcc cacaaggaga atttatctca 13080 aactctagcg gctgcggcaa gggcgtcttc ttcagacatt ctcatttctg ccttgctcct 13140 ggtgagtcga ttcatcgcca ctgatgtagc ataagcgcag cggctgaacc aggcaccagc 13200 tatgcttcta catcttgact gttggggtct acaatgtcta tttccacaaa ctttccacgt 13260 ttcctgggcc gcttttggca agatgcagct tggtaagaga cccctgagct ctgcgccctc 13320 agcgatgttg agattgactt tgaggtagat atggcgcatc taccattcgc agactggtcg 13380 ctatcattgt gctatcgagg cgcagcaccg taaatacggt aagcgcttgg gggcttcctt 13440 gaattgaacg cattgctgaa gccacgtagg gcctgtcgtc agagtgtcgc ccaacgaact 13500 gtcctttgcg accgttgagt cgtggaaagc catctacggg catcgtgttg gcggcgcacc 13560 cacacccatc aagagcgagt tctacgacat atatggggct ggcttcaagt ccctgtgtat 13620 cggaagcgag cgtgatccgc ataagcatgg tcagatgcgc aagatgcttt ccgccgcctt 13680 ctccacgagg gtgttgacag aacaagagga cattatcacc aaagcagttg accaattcgt 13740 tgttcgactt ggcacagatg gagcgcaggc taacggtctc aacatgacca aatggtacga 13800 aatggttgct tttgattcct tgggcgaaat ggcttttgga gagtcgtttc acagcgttga 13860 ggctggtatg tttctaacaa gcaaatttgg tcttgttgtt ttactcactt ttccgttctt 13920 caggtaaacc acatagctgg tcggagatca tagtcagcca tctctacttc attaccttga 13980 ttgacaacct gcgccgtctc cctttcgtcg ccaggctggc taggctgctg tttccatcaa 14040 cgctagcggt gcaaaaccag aattctcagt acagccgcaa aaaggttgaa cagtgagtcg 14100 gacacgacaa attctacaat ttgtcagact aatcgcatga gacaggcgtt tatctaaaag 14160 ctctacccgg aatgacttcc tctccaccgt tgttaggagc tatgaggag ggaccgtgag 14280. 14280. 14280. 14280. 14280. 14280. 14280. 14280. 14280 ttatatgata gaagttgcta gtgtgctaac tcaaatgcag cattgctggc ggagagacca ctgcgacatt ccttgctgcg gcgacatatt atttgctgcg caaccctgct tgtctcactt atgttcagga agaaatcagg gccaagtttt cttcgtatga ggcgattaat gccacagcag ctagacaact tccttacctg caagctgtaa ttagcgaagg gctcaggatg tacgcgcccg gcagccaagg cttccccaga ttgtccacag gcatgaaagt aggagatgtc tatgtcccag ccggcgtaag cacagccctc tgagcatacc ccgcctactg tactgatgtg gctcttaggc tgaggtgttt acacacgcct ggacgttgac tcatagcgag aagtacttct ctgatccgaa aggtttaaa cccgaacgct ggattgatcc ccagtccacc gatgtgaaag atgctagcca gccgttctcc atgggaccaa gaggttgctt aggccagaag tagttctga cgagtcgcgc acttagctgc tagtttacga caaggatttg gagctgacat gtgcccagct ttgcatatat ggagatcaac ttgattctgt ccaagatgct gtggaaatat gatctggagc ttgtgaacaa 14940 gaatttggac tgggagcggg agagtcattt gcatgtgatg tggtggaaac ccgcattgat 15000 ggttcggttc aaggagagaa ggtctccatg aatgcctcca tctatcttga acgattatga 15060 tgagactccc gtcttcatcg gcaaagtagc cgatgttcag gagcaacctg ccagcaccgc 15120 aacggcagtg tctctcccca tcgcccctca cttcaccccg ccaccgtctc gtttcgtggc 15180 gaaatgtcat cctttggaga agcaaatcac agctgaagtc gatggctact ttctcaccca 15240 ctggcctttc aagacagcca aggacagaaa gaagtttgtc gctgcaggct tctcgcgtgt 15300 cacatgcctt tactttcctt tgggcttaga tgatcgcatc catctcgcct gtaggctttt 15360 gacaatcctg ttcttgattg atggtacgtt gacccccgaa caatccgttg attacatgtc 15420 tgattttgaa tatccgtaga tatactggag gacatgtctc tggaagacgg atgtgcttac 15480 aacgagaagc tcatgcccat cgcccggggt gatgtccagc cggaccgcaa tgttcctgtg 15540 gagtggatca tgtacgatct atgggaaacc atgagatctc atgataggca actggccgac 15600 gatgtgctgg agccgacatt tactttcatg cgtgctcaga ccgacaaggc tagattgtcg 15660 atccgtggca tgggccccta cctgatctac agagagaagg atgtcggaaa ggcgtaagta 15720 caaagcctgt gtggaatgat atggaggcat ggcattaatc atccccgtac cagactactc 15780 tgctctctca tgaggttttc gatgggtctc cgcgtgtcac ccgaagagct cgcatcagtt 15840 caacccatcg aacaaaacgt cgccaagcat ctctctgtcc tcaacgatat tttgagctgg 15900 gacaaggagt ttctggctgc gcagacgggt caccccgaag gatcggcgct ttgctcggcg 15960 gtacaggttt tggcagatga gacgtctctc acatacgcag cggcgaagag agtgctctgg 16020 gtcatgtgcc gcgagtggga gtatgagcac gactatcttg ttgagaagcg cagacaagat 16080 ggggagctcc cgcctagcga gaatctgatg footcatca agggattgga gtaccaaatt 16140 agcggaaacg agatttggag caagaccacg ctgaggtaca acgctcccac aaccgcttga 16200 ctatacggag aaggggcagc ctacagctaa cttcgatccg ctatatacgg gaagtgactg 16260 ctcaggcgca tatgccgtgg cacttgaggt gatgacaatg ctccctgggc actttcggcg 16320 acggaaatgg ctgacggaga gttttgcaaa gtttgcaact gcgcggaggt tgatgtcgag 16380 tactcgatac tcgggctcga tcaggtcccc agtaacttcg tcaaacgtgt cggcgaaata 16440 gtcaacctct tccgagacgc ctgcgttgga aacggcgatg tcaatatcgc cggcatatcg 16500 aaaggcacct agaaggctgg accagcttga aatatcgcac tggcgaaact cgatgccgtc 16560 tggttgatcg cctggtggcg ggtttatgtc gaggttaagg accgatgctc ctttctgtgc 16620 aaatatgtgg gctgcagcaa gtccgatgcc tgatgatgcg ccggtgatga tgacccgctt 16680 cctgtgcaaa gtagggatgt cttctagagt gaagttgagg gatgtcatct aatcatggcc 16740 aatagccaag agtcgctggt ccctgacgtc gtcgcgtctc tcggtaccga tcccgaacga 16800 agtttctccc gcacgcccat ccccgtcgac cttggccagg agaatatttg ttacctcgac 16860 aacgacgccc agcaggacga agatagccag aacttggcca aatgtccact ttttctcctc 16920 ctccgacaga ctgttgcgtg caacaatctg ttccaaagtc acaatcatgt agacattgat 16980 tcctgtatag atcaacatct caaccagctg ccgggataac tcgtttctc agttatccgc 17040 tatcttaacc tgacgccaaa ggtttgtgaa catctgcaca tcctggctgg tcggtctccc 17100 ctgtctacca cagatgcagc gagcaaagat taccgaaaga atcatgctca tgacccaacc 17160 tacgaccatc gacgccataa aagctagtat aatgtatcgg aagacatcgt ctgttgcctt 17220 gatacttatt ccaaagacga catacagcgt tgaagagttg caggtgggtt ggctaccaaa 17280 cgtcggggcg gtaagccaaa tgtaagcggt gagggcgatg aaggcgcagg cgatgacggc 17340 ccggaaaaac gtcaaaatgg cccaacgctt gaccccgaca ccggaatact tccgctgtgc 17400 cacaagaccg aagccgagga gagacagaag atgaaggacg caaatggcgt gaaacaaagt 17460 gagtggcct tggaaggttt gtactatggc ggtacaaagc agcgcgagtc cctgaagctg 17520 caaagcagag ttgagggatt ggcaaaactc ttctagtttt ttcggatgt gatgtgcttg 17580 atcaagtttt taagtacgcc agaggccaag caaagtgtgt agatagaagc gcgaatctga 17640 ggtatggtca gtatggtgat gaagacccag cgaaattgtt taacgtactc cggtcccagt 17700 gacgtccggg ttaggttcaa tcacacaagc cattcaagtg cgtgatgaga attctaagct 17760 accttggttt gctgccattc tcgcgaagac gtatccccca agcgcgttgg agccaatgag 17820 gaatatagac cacttctgca acagagtaag ctgatggtca tcgcgtattt tcacactcgt 17880 tgttcctctg aaacctatct tgtcctacgg ttgacttacc caacgaaaat aatccacctt 17940 gccttcttca tctggcttcg gccgtaggtc acgaattgtc tcaaacacaa gtggaaatgc 18000 tgccaccaca gcagagatga caagctcctt aggcgctgcc tttctgtaag tatctgtaag 18060 gatcaaacgt ggtttgactg cacttactgc ttccaagggc gatatttcca acttctgtga 18120 ctagcagggg gcagatgcca aacgaagacg caatcaagaa cagcagaacg gtaaagacaa 18180 cgttaacact atatgtaagt acgacctccc gaaatgtcgt tattgcgctt ttcgtgaggc 18240 ccgccagagc tcccagttgg aggactaaga ctgatggtgg gatgccgttg agcgtggcac 18300 caactcgggc cgctgccacc ccgccggcgg cattgagagc catgtcgatg atcgctagga 18360 ttccgaatcc ccagactgtg agaccgtgta agtataagta ctttccgggt tcaattgcat 18420 atccttactg gctcgccatg gtgcccgatt gacgatcact agcggaggct gatagttgac 18480 cataccactg agagggtggg gatttccaag cccaatgccc tccatatgtc tggacaacag 18540 ggacgcaaga ttgccattgt cggtgccagc ggcaacgttg gtggcggcac cctcaatgct 18600 ctcctcgcca aaggcattca caccatcaca gcggtcaccc gcaccgagtc aactgccata 18660 ttccccgagg gtgtgatcgt gaagaaggga agctacgagg acgaatcctt cctcgtctcc 18720 gcattccagg gccaggatgt cgtccagctc gccctcggct tctccagcta cgcaacgggc 18780 caagagcctc tgattcgcgc cgccgctaag gccggtgtga agtggatcct ccccaccgag 18840 tacggctccg ccccggaccc ttccaagctg ctggattcca gccctatcct ccagggcaag 18900 atccagatcc gcaagttgat tgaagagctc ggctctagct ggatcgccat tgtcaacaac 18960 ccctggtacg attggtccat gggcgccggt ttctggggta tcgacattaa gaaccgcacc 19020 gccaagctgt acgacggtgg aaagaccaaa ttcatcacga ccaacattgg cgtcaccggt aagggtaccg ccggtgttct gagcctgccc gacgccgagc ttgagaagta ccgcaacaag cctttctatc ttaagtcttt ctacatcagc cagcgtgaca tgctcgacag cattcagcgt gctactggta ccacggataa ggactggaag attgaggagc ctgacgccgc cactgttgtt gccgaagctg aggaggctgc aaagaagggc gaccacgtcg ctggtctgat ttccttctat 19320. gttaaccaca tgagggctgg ctggggaggt gactacaatg acaaggttac tgatcttact aagattgggc ttgaagccga agatctcgac gagactacca agagagttgt caaggatgtc gaggacagt agatggatac cataggattc attcgtgctc agtggaaaaa ccttcccatc gtccccaca agcagacctg taccggcaaa acctacatca tcacagggtc aaactctggc attggctttg aagctgtcaa gcaccttgtt aatcttggat catctcgtgt catcatcgcc gtcagatcca ttgagaaagg tcaagaggca aaagcccgta ttgatgcctc cactggcaga ccgaagatca ccgaggtttg gcagcttgac atgtcatcct atgaatccat ccgagccttt 19740 gctaaaagag catcgtcgga gctcagtcgt attgacggcc tggtcgaaaa cgccactgca 19800 gctttggata gatggacagt atctgaaggc atggagacaa gcgtcactgt caacgtcatc 19860 ggcacagtga tgctagcact gcttcttcat ccgaagttga tggaaacggc caagatttca 19920 gactcgcagc cccatatcgt ggttgtcacc tctggacttg gtttcacgca gccggacttt 19980 ttggatcacg cagataagga tgtctttgat gtcttgaaca ctcaagggga gtctcccatg 20040 gagaaaaggt acgtcacaag ccttctgact atcatgtaca cggcttgctg actgaacgac 20100 tgcagatact ccgttacaaa gcttgttcag atacttgcca tccgacaact cgcgaccatg 20160 ctaccagcat ctcgtaccgg cgttgtcttg aacctcctca atcccggtct ttgcaacacc 20220 ggcctagcaa agtatggcag aatttcccta cggcttcagg ttcggatgtt caacgccctg 20280 attggaagaa ctccggagat ggggagccgc acgatcttgc atagcatgtt tgctgggcca 20340 gagagccatg gtcaatatgt ctcagattgt gagattaaag agtacgtcta catcatcaag 20400 cactga 20406 <210> 5 <211> 1378 <212> DNA <213> Artificial Sequence <400> 5 atgaagatcg tcgtcgttgg cgccggtctc gcaggtctct cgaccgcgct ggctctctca 60 ggttcctcaa caaaacaccg gatcatcctt ctcgaatcag ctccagcgct cgccgagctt 120 ggtgcaggcg tgcaactaac acccatcgcc acccgacagt tcaaagcatg gggtcttgag 180 ccccaactcc tcgccgcatc cgctctgcct aaggcatgga atctacgccg aggctcagac 240 ggagaggtgc ttaaccgtgt gccgatggac aagttcgagg aatggtatgg cgcgccatat 300 gtggtcgtac atcgtgcaga tctacacagg atcttgcatg aagctgtcgt agcgaagggt 360 gtggaagtgc gcttgaacag taggattgaa cagtacggga tagaagaagg atgggtcagg 420 ctaatgaatg gtgagaggct ggacggagat ctggttgtgg cttgtgatgg catcaatagt 480 tcggctagaa cgcagctttt gaagagcttc gggagcacat tggctcgaga cgaggagctt 540 gaaaccacgg gatgggcagc gtatcggctg atggctgatg ttgaagatgt caagggtgat 600 cccttgacga aagatatcgt gagcgaacat gctggcaact gctggtgcga catctctttt 660 gctcgaatct ggaatcagga gactaacggc cgtcagggcg gacgcaagca agtcagtaat 720 gacctacatg gttcgcgact ccggaaaact caaccttgtc ctatctcacc ccgatgatgt 780 tgacacaagt acctggacac gcgaacaata cctggcggag cttgtacgct tctatggcga 840 cattgatccg tgtgcgcgac ggctcatcga actcagcacc ggtcccatca cgaactggcc 900 ggtccatcaa gtcgcgaaat tgcctacctg gacgtcaaag actggcaagt ttgtacttct 960 tggtgacgca gcacacgcca tggcgttcta tctcagtatg ggtgtctcgc tggccattga 1020 agatgctacg gcgcttgcca cggctttaga cctgcatgtc aaagacccta caaacgcgtc 1080 cttagtagaa gcattggagt tgtttgagaa ggtcaggaag ccaagggttg agaagattag 1140 ggatgcaagt ctacatgctg ggacgatgct gcacctgcca cccggctatg agagagaact 1200 gcgagatcag tctgcgagaa acgatggtgc gatcgacgga gcacgggatg gagatgcgtt 1260 actcggttgg gtcagctacg gtattaccga caagacaatc cgcaaagagt gctatgggta 1320 cgatgttatg gctgcaatgc ttgagcaggc aagagcagat ggactcttgc caatgtga 1378 <210> 6 <211> 862 <212> DNA <213> Artificial Sequence <400> 6 atggtcgtca cagtacccac tgcctccctt gaagggcagg ttgccctcat caccggcgct 60 ggtcgtggaa tcggccgtgg ctgtgccatc gagctcggca agcgcggtgc ctcagttatt 120 gtcaactacg tctcgtcaga gggtcctgcc caggaggtcg tcaagatcat tgagggcttc 180 ggcaacggtg ccaaggcggt tgctatccag gccgatgtca gcaaggtctc tgaaatcacc 240 agactgttcg aggagggcaa gaaggcattc ggcaagatca acattgtcat gtccaactct 300 ggcactgagt cgtgggatgt cactgaggag atcaccgagg agaagtacga ccacgtcttc 360 aacctgaacg cacgcgcaca gttcttcgtc ggtcagacag cgtacaagta catcgaggac 420 aatggccgca ttatcctcat gtcgtcgatt gctgccggtc tgttgggtgt caaggaccat 480 gctctttaca atgcgtcgaa gatggctgtt attggcttca tcaaggcatt tgccacagac 540 ttcggcaagc gtggcatcac tgtcaacggt gtcgcacctg gaggcatcaa gtcagatatg 600 ttcacacaaa acgcttggta agtgacgttc aatcattgac agcaccacgg acatttgcta 660 acgctcaaac aggcattaca ttcctggcgg ttcgcccgac tggccagctg agaagatcga 720 gggtttgatg gcaagccact gcccgcttgg aaggtgcgcg gtacctgagg atgtcgcgag 780 ggtggttgcc ttcctggcaa gtgaggacgg tggctgggtc aacggtcagg tgctgaccat 840 ctctggtggc tcgtcgcagt ag 862 <210> 7 <211> 1474 <212> DNA <213> Artificial Sequence <400> 7 atgtctcctg acctgacata caccgaagtc aatcgtaagc atccacgaat ctatcgttcg 60 tttatatcgt ctacgttatc tgacctcaaa cagagaatct tgctgcgcga gagaatgcct 120 cctggttctc accggttcgc tttgcttatg actggctcga agatgcaccc atcgaacacc 180 tcaccgcagt gcctctgaag atacgcacag actcacctct cgcctcgcca aagccgagta 240 atgtcaaaga acagagcatc actgagaaca acgagaacag ctttagtatc tcgcctcagt 300 taaccggctt gccctggccg acgagtttca ccaaagttcg ccagaatcgc cattggcgcc 360 agagtttgg tatctccacc caactcctcg agctcttcgc tgccgatgac acatccgcgc 420 aggctgtcg gcgtaacggc gttcgcttg cacgtatagc ttcacatgag ctccagaccg 480 acgaggagga ccgctttact aagtttgcta catacatctt tcccgaagca aatgaggagc 540 gcatgaaact actggctgcg accatcgtat atattatcat cttgacggt aagcgcgcgc 600 atcaccggcc tttatgcatg catgtctgaa caggaacgca gattcatggg aaatgcatag 660 cgaagatacg gtaagtaccc cgcttccgta tttgcctctg tccattgtcg cttactagag 720 tgtgctaaca cctgcatgaa gctcggtctg gttcgggatg attttatcag acggctgcga 780 ggtgatatcg agggcatggc tgagcatcag acgcccttgc agcaactcat caactccaca 840 gttcaggggt tcaaagacca ggacaaaact atgggcaacg gtggacaaga agtacttgac 900 cgtttgatcg acttctgtga acatgtgcct ccccagacga aattcgcaac aatgggtgat 960 tacctgagct accggctgat tgatgtcgct tttccgtaag tggttccatt ccaatattga 1020 tcttggctga tctcgtccct ataatggcag gtatctactt gcgtgcatca agttctccct 1080 cggctcgagc gtcaatgttg aagacccgaa gcttgcgccc attttacggc tggtgagcga 1140 ccatgtctca ttggtcaacg atctggcgtc ctatgacaag gaaaagcggg catacgataa 1200 tggtagcgca tgctacctta tcaacgctgt agacgttgcg cagcgactct tctcactgcc 1260 ctcagccgcc gaagccaagg cactcacgta ttctatgcag ctccttgttg aagctcagat 1320 caagacggaa cttgacagtt tggtggctgg aggaatactg tcttgcgagg agcttcgatt 1380 cctcgatgct gcgctactca tggcctctgg aaacgtgttc tactcagtgg tctcgtcgcg 1440 atacggtggt aaggcagcca agctggaaaa atag 1474 <210> 8 <211> 1474 <212> DNA <213> Artificial Sequence <400> 8 atgtctcctg acctgacata caccgaagtc aatcgtaagc atccacgaat ctatcgttcg 60 tttatatcgt ctacgttatc tgacctcaaa cagagaatct tgctgcgcga gagaatgcct 120 cctggttctc accggttcgc tttgcttatg actggctcga agatgcaccc atcgaacacc 180 tcaccgcagt gcctctgaag atacgcacag actcacctct cgcctcgcca aagccgagta 240 atgtcaaaga acagagcatc actgagaaca acgagaacag ctttagtatc tcgcctcagt 300 taaccggctt gccctggccg acgagtttca ccaaagttcg ccagaatcgc cattggcgcc 360 agagtttgg tatctccacc caactcctcg agctcttcgc tgccgatgac acatccgcgc 420 aggctgtcg gcgtaacggc gttcgcttg cacgtatagc ttcacatgag ctccagaccg 480 acgaggagga ccgctttact aagtttgcta catacatctt tcccgaagca aatgaggagc 540 gcatgaaact actggctgcg accatcgtat atattatcat cttgacggt aagcgcgcgc 600 atcaccggcc tttatgcatg catgtctgaa caggaacgca gattcatggg aaatgcatag 660 cgaagatacg gtaagtaccc cgcttccgta tttgcctctg tccattgtcg cttactagag 720 tgtgctaaca cctgcatgaa gctcggtctg gttcgggatg attttatcag acggctgcga 780 ggtgatatcg agggcatggc tgagcatcag acgcccttgc agcaactcat caactccaca 840 gttcaggggt tcaaagacca ggacaaaact atgggcaacg gtggacaaga agtacttgac 900 cgtttgatcg acttctgtga acatgtgcct ccccagacga aattcgcaac aatgggtgat 960 tacctgagct accggctgat tgatgtcgct tttccgtaag tggttccatt ccaatattga 1020 tcttggctga tctcgtccct ataatggcag gtatctactt gcgtgcatca agttctccct 1080 cggctcgagc gtcaatgttg aagacccgaa gcttgcgccc attttacggc tggtgagcga 1140 ccatgtctca ttggtcaacg atctggcgtc ctatgacaag gaaaagcggg catacgataa 1200 tggtagcgca tgctacctta tcaacgctgt agacgttgcg cagcgactct tctcactgcc 1260 ctcagccgcc gaagccaagg cactcacgta ttctatgcag ctccttgttg aagctcagat 1320 caagacggaa cttgacagtt tggtggctgg aggaatactg tcttgcgagg agcttcgatt 1380 cctcgatgct gcgctactca tggcctctgg aaacgtgttc tactcagtgg tctcgtcgcg 1440 atacggtggt aaggcagcca agctggaaaa atag 1474 <210> 9 <211> 1679 <212> DNA <213> Artificial Sequence <400> 9 atgcgctctt ttgctgcagc aacacgatcg atcgcacgcc agcagacaac ctgcagaaac 60 agcttccagc gccagacact gccctcgatt gtatcaagga catattcgag caagatgggc 120 cacaacgtgc ctgccgtacg ttcctagcat cccgtcccat ctgtgtccgg acgtgacgag 180 caacagcgct gacactccca cagctcaagg acccctccct tctcaagagc cagacataca 240 tcaatggcga atggatagac gccaaatccg gcaagacctt cgaggtccac gatccagcga 300 ccggcaagct catcggcacc cagcctgaga tggaccgcac cgacaccgaa gccgccatcg 360 ccgctgccgc agctgctctg cccttgttcc gcaagttgac cggtcgcgag cgatcgaggc 420 ttcttcgaaa atggtaccag ctgatgaccg agaatgccga cgatcttgcg acacttatca 480 catgggagaa cggcaagccg ttggcggatg caaagggcga ggtcacgtac gcggcgagct 540 tctttgaatg gttctctgaa gaggcaccga gggtgtacgg gaataccatt ccttcgagcg 600 ttgccacaaa cagagtattc acagtcaagg agccagtcgg tgtgtgcggt ttgatcacgc 660 cgtggaactt ccctgctgct atgatcactc gcaagatcgg tccggctctg gctgctggct 720 gcactgttgt tgcgaagtct ccaggcgaga ctcccttcac tgctgcagcg ttggccgagc 780 ttgcacaccg cgcaggcatc cccaagggtg tggtcaactt tgtcacagct atggagaaca 840 cacctgaggt tggtgagaca atcacaacca gcaagaccgt caagaaggtc tcttttaccg ggtctactgg tgttggaaag cttctgatga agcagtcgtc gtcgactctc aagaagctgt ccttcgaact tggtggtac gcgcccttca ttgtcttcga cgatgccgat ctcgagactg ccgtaactgg tgctatcgcc tcgaagttcc gctcttcagg ccagacctgc gtgtgcgcca 1080 acaggatcta cgtacagtcc ggcatctacg accagttcgc tgagaagttc actgagaagg tcaagggctt caaggtcggc gcaggtttcg aggagggtgt aactcacggc cccgtcatcc 1200. acgaccgcgc agcgaagaag gtcgacgacc atgttcagga tgccgtcaag aatggcggca 1260. aggttctgat cggcggacag aagctcccag agctcggcag ctctttctac caacccacag 1320. tcatccgcga catgaccgct gacatgcagc tcgcgcacga ggagactttc ggccctgtcg ctggtctttt caagttcgat accgaagccg acgtcgtcaa gctggcgaac gcagcggatg taggtctcgc cggatacttc ttcagcaagg acatccagag ggtgtacaga gtcgcagagg cgctcgaggt gggtatggtt ggcgtgaata ctggtctgat ctcagaccct gccgcgccat 1560 tcggtggtgt caaggagtcc ggcttcggcc gtgagggcag catgcttggt attgaggagt 1620 atgtcacaac gaagacaatc accatgggcg gcaacgggca gccgctgcag ggcgagtaa <210> 10 <211> 220 <212> DNA <213> Artificial Sequence <400> 10 atgaccattt cactcagcta cctatcgctt acagcgattc ttgtatcact tggggcagct 120. cactttgcat accttgtcat ctggcggtta tactactcac ctctatcgag gttcccaggt 180. tctcaactcg ctgcagccac gggctggtac gagttctact atgacttctt tcataaaggc aagtacatct tcgaatcga gaggatgcac catgtgtatg <210> 11 <211> 1198 <212> DNA <213> Artificial Sequence <400> 11 atgcgatacc aatattctga gagggtggag agccatcgat atcgtgacga tggactggcc 120. aacaacatac atctacgtat acacaaggat tcttacaaag aggtcattgg gacattgcgg gctcagaacg attggagtcg attggtcagt tctatgacga agtaccatgg cgggctgggc 180 gacctgttca gcttcataag cgtcaccatt ccagaatgct taccagagcg cctcgaagtc 240 gtggcgtacg caaacgagta tgcctttctt tacgatggtg tgtcacagct gatcccgcga 300 tctgctgcct cttgtcgtgt atctgatgct ttgcagatca aatggaacga ctggatctca 360 aagatgtcag ttcgagagac gatccataca acagcactgt tcactgaccc catcagtttc 420 gcgaaggccg ggacgatatg ctggacatat ttggtataca tggtggagcc agcaacctcg 480 aggaccgccg acctgagaag accctgcagc tgcagatctt cgatgagttg atggctattg 540 accaggatcg tgccatcgtt acaatgcaag cttgggcaaa gttcattgac cttgcatctc 600 gaacacgcgt agagcccttc aatactctgg ctgcgtacct gccgagcaga accattgatg 660 cgggcgagct gtaagcactc aacagtctcg ttacacagga tcgtggtccg gctgacgtat 720 tagattctgg tttggcatgc tgactttcgc aatggcgttg acgattcccg cgcacgaact 780 tgatgtatgt atgcgcctgg ctcgaccggg ctatgaagct atcagtctca tcaacgatat 840 atattcctgg cccaaggaac gtgctgaggc ggagaaagct ggccaggatt acgtcttcaa 900 cgccgtatgg gtcgtgatga aggagcggaa atgcgacgag caaaaggcca ctgaattctg 960 caagaatcta gcccggcaat cgattcagga cttcagcaca agtgtgaaca caccgcaggt 1020 cacggagtta tcatgtgaca gtcgtaccta cctgggagct gtgagactga gctatgttgg 1080 gaaccttgtg tggagcatct actgtccccg atacaacatt gcgtaagccc caatatttgc 1140 cattccactg acgagctaac gtaagcgcag ggtaccagtc taccacagta agctatag 1198 <210> 12 <211> 395 <212> DNA <213> Artificial Sequence <400> 12 ccagatcacg ttgtcatcag cactgttcgc gacccaaggc ttcgaaggcg gaggaactta 60 agagcctgcc cacagcaaac ggctcacgtc aactgcttgt tggtgtcgag aacacgaatg 120 ttgaggatcc caagaaagct gttgaggcta ttgaagctgc tggaatcgat cacatcgatg 180 tcgtcgaggc caactccggc gtctctattg gcgctgctcc cctcgagata gctggcccca 240 aatacttcac cgagagcttc aacatcaacg tcttgtccgg cgtcgtgctg taccaggcag 300 tgagtagcct gcccagaaag tctaacgctc cgaagtggat ctcggtctcc agtcgaggtg 360 gatccacctc cgctccactt ccatggtacc cgtaa 395 <210> 13 <211> 1729 <212> DNA <213> Artificial Sequence <400> 13 atgaataatc aacttttact ggccgtggcg gcgtttgccc tatttttcta cgttgctgca 60 gcattgttca attgggtgcg ctctcctttg cggtccatac ctgggcccat gttggcgcgc 120 ttcacggact tttggtattt cttccgaatc aagcaaggca gtttcgaaaa ggtcaacatt 180 tctcttcatg agaagtatgg tatgtctatg tgcagagatg tcaggcttgt ctgcgtggca 240 cttgctgatc ttgaggtgca ccccaattag gccccatcgt cagatacggc cctaatcgat 300 acagcatcga tgacgctgag gcatccaaga ctatctatgg tcatggaacc cagttcccta 360 agtcgatctg gtactcatca tttcagccaa atgacacttg gtggaacatt ttctccgacc 420 agtccatcaa acgccacgct cacaacagac gcttcttcgc gcacgccttc tcgatgacat 480 cgctcgttaa ctacgagccg tatcttgatg agtgcggcga gatcttctcc caacgcatgc 540 tggaattctc aaaagccggt gcacctgttg acatcggcca ctggtttcag tgctttgcct 600 ttgatgccgt tgcatatatc acgtatggca agcgactagg cttcctggac cgcggcgatg 660 atattggcca ggtcatcgag aaccttgacg ccagcttaat ctactcaagc ctcgccggaa 720 ttttcccttc attccatcgc tatattgccc cggttctctc agccctgggc cctcttttcc 780 gaactgaccg catcatgtat gtgatgaact tcactcagag cagggtcacg gaagagcgag 840 cttcgcccaa gtcggtcgct gagtccaaga ccgacgtaga aggtgcaggt gtcggtgaga 900 ccttcttgag aaagtatctg gctaaacact ccgaggaccc cgatgctttc accaattacc 960 acattctcat tggctgcgca accaatatgt ttgcgggctc agacacaacc ggtattagtc 1020 tttctggaat cttgtaccat cttcttagga acccggattg cgtcacaaag ctgcgagccg 1080 agatcgacga tttcagcaac cgtggcgagc tctcggagaa tcccacgttc aagcagggtc 1140 aggacatgcc ttacttgcag gctgtcatca aggaggcgtt gcgtatgcac cctgcagtcg 1200 gcctgccgct ggagcgagtc gttcctgagg gaggcgccac catcgccgat cgtttctttc 1260 ccgaaggtgt acgtactgat gacttttgag ggaatcggtc attttactaa cgtcttcctg 1320 tcctttttag tctgttgttg gcatcaatag ctgggtccaa catcataaca agagtatctt 1380 cggacaagat gccgacaagt tctgccctga gcgttggctc attgacgacg aagacaagct 1440 ttccccgatg aaccgaaact ggatgccggt aattgcggtc ttccaagctt ttgagtgagc 1500 ggtttggcta attttggcat agttcggctt ggggtcaagg acgtgcttgg gaaagaatgt 1560 ttccattttg gagatgacga aactgatccc gagaatcatc cgcgatttcg acttcaagct 1620 tgacggagac gcggcagcac ctggagggtc ttggacaact gccaatgcgt ggttcgtgaa 1680 gccccgaggt ttctgcgttc gggtagagcc ccgaaagctc agcgcttga 1729 <210> 14 <211> 1035 <212> DNA <213> Artificial Sequence <400> 14 atgtctcaat cctacaaatc aagcagcctt tttgatatcc gagggatcgt agctgtcgta 60 acaggcggag gcagtggtat gtccgcattc accataactc tttaatcatt tggctggcta 120 atcacatttg ctgcagggct aggactttac atgacgcgag cgttggcaga aaacggcgca 180 aaaaaggtct acatccttgg tagaaggaaa aacttgctgg atgatgctgc gaaagaaatt 240 gtgcgtaccg tccaatggtc gccaacggtc caacgcccgc tgacttatag cagggctgtc 300 acaacgttgt accgatccca tgcgatgtga cggatcgcca gagcctcaaa gcggcagtgg 360 cgatgattga aaaagatgtt gggtatatta acctgctcgt tgcgaactca ggtattgccg 420 gaccttctgg aaacgtgcct gctggcgcct cgattgcgca agttcaagag gatctcttgg 480 agattcctat ggaggagttc accaacacct atcacgtcaa ctgcactgct gtattttaca 540 cggcgatatc attcctaggc ctcttgcaca agggtaatca gtccaattac cagggtggaa 600 ggagccaggt cattgccacc agcagtatcg gcagcttcaa caggaagatc actgctgggt 660 ttgcgtacgg cactagcaag gcatcaacga cgatgatgtt gaaggttttg gcaacgtatc 720 tagtgccgta caggatccga gccaatgtac tctgcccggg atgtaagttg gcgtccggca 780 aacatagttt ctggtcaacg atgctaaaaa tgtcaagtgt tcccaactga cctcactacg 840 agtctaattc aaggcacgga tcctacaatt gagggggcct ttcctgtgga tcaaatacct 900 gctgagagag caggtctccc ggaagacatc atgggaccgc tactgtatct ggcttcacga 960 gccggcgcat actgcaatgg aaactgcgtc ttgacggatg gtggcaggct cagcgttact 1020 ccggctacgt actag 1035 <210> 15 <211> 1929 <212> DNA <213> Artificial Sequence <400> 15 atgcctacca gaaagagaga ccagaacagc agacatcaca ctgcggccag tctcccgcct 60 tctccaacga cttcagactc ggataagtct catgacacct tgtttattga gacgcaaatc 120 tctgctaccg ataggttgac aacgaagcag gcactatccg cgtcaatcac agaccgtgag 180 gtgctcgtac ctgacatgtt cacatcaatt ctgtctgtcg agcctgtaca gaacgctcac 240 tacgcccaag ttaagctaga agcggatttg tggatcgcta agtactcact tctgacactc 300 gatatgtaag atttatgagt gacctaacac tcctaatctg tcaagaaccc taggtctgaa 360 cgatgagcag aagtctcgtc actctcgcgc agacttcgcc tacctggtgt cttggtgggc 420 tccaagctgc gatgcagaag ccctgcgtac catggttgac tggcagaatt gggtaagctc 480 tatcagcgtc gaaagctttt tttctgaata tagaatgagg catacggtgg gagtgggcta 540 acaaggtctt cattatacgg ggcgcaggca tttgtttggg atgaccgtaa gtcacatcct 600 tagctttgtt ctgatgttat acgaattcaa tctaagttaa aacatgcaac taagctaact 660 tgctgtttcc gacacagagt tcgacgaggg tcatttgaaa gaagaccttc acgctgcagc 720 ctctaacatc atcaacatga cctcgattct ggatgattgt catcctcttg tctgtcgtga 780 agacgatcca attgcatatg cctgtcaagt caactggcgt agcattcaaa aggtacctta 840 cttctatctt tcaagctgcg gcggacagta gatcactaac aaagttccca tagcgcgcat 900 cgcctggtac gcggtgtctt cactatatcc aattgagagt gggatggaat gctaatgtaa 960 cagcctgaag ctctccaaca tcgatacaaa gcctgtaagt tgtgatccca agcctgctat 1020 gtcaccgcgt catcgattga ccaataaaaga cctcaaacac tacatgctcg gagtacttgg 1080 ccaagtcaac tcgcgaaaat tggatgctcg aagtctcact atcgaagaat ttctagtttt 1140 tcgacgcggg accattggtg tcatgccttg cactgtgctg gtcgagtaag acgcctcgaa 1200 aagatggcca attatcgaat ttgtcaggaa ctgagcattg ctgacctaag cgacggcagg 1260 tatgcgctgg gaattgaagt cccagagcat attattaacc acccatccat caaggcttgc 1320 caggaagtag cggtggactt ggttctcctg ttagtttatc attcaagtga catttacacc 1380 gagtgacagt actaaccaca ccgctcctag tgataacgac attctctcat acaagaaaga 1440 cctggtaggc tcatttcata tgatgattta attccgcaga acttgtacac gttcgctgat 1500 aaacgtatga gaacgcattg ttcctaacaa tttcatagat cgagggagag gacttgtctg 1560 tcatcggtat cttgcgctct caaggttaca ctctacaaga agccatggat gaggcaggac 1620 gtatgattga tgtgagatac cggcgatggt atgaggcttt ggctcaaatg ccgagctggg 1680 gctccaaaca agatcgcttg gtgctcaagt accttggggg ccttcgcgac attgccctgg 1740 gaagtcttt atggaggtaa gactggccac aatacctaca acataccccc agcttccact 1800 ttctctgatt catgcattct tattaacatt tctacctccg ctgccagctt ttggactggt 1860 cggtacttca gtaaacaaga gggtgagatg ctacgcgaaa cccgtctgct gcgccttccg 1920 attcagtag 1929 <210> 16 <211> 590 <212> DNA <213> Artificial Sequence <400> 16 atggtgtctc ttgccgactt gcttcctcct gcgaagggta tcctgcccta ctacatgctc 60 ttcgtaagtc tcgtgtcact ggttataaca ccacgcgcaa gatcgcgaag ctaacccagt 120 gccaccaaca gctttccctc cttgccattg gcaactcggc ccagaactac ttgaccctcc 180 actactcgcg ccgcgtctac aatggcaaat tcgtcaagaa ccctaccctc cctcctcgct 240 ccgaaaagta caaccccgag gactcggtca acaagttcgt ccccgcgccc gccggcgcaa 300 aggagtccga gaccctggac cagggcacac cgctcgccgc ccgctgcttc ggcacctgga 360 ctctgttgac aagcgtcgtc cgcctgtact gcgcctacca cctgcactac gcgcacatgt 420 acgacctcgc catctggaca tatgtcattg cgctgggcca ctttgcgagc gagctcttcg 480 tcttcaagag catgactttt ggcgttcctc aagcgttccc cttcacgctg gccaccgttg 540 ccctgatctg gatgccccag gttcgcagct tctacgtcga ggccccttaa 590 <210> 17 <211> 1668 <212> DNA <213> Artificial Sequence <400> 17 atggagtacg gcatcctcct tctgggcttc cgccctttct atcttttggg cgtctgctcg 60 cttctctctt tcttgttcgt atggattttc gcgaagaaac cttccgagtt ggatgcgcca 120 cttttgacca acgactctgg agacttcttg caaatcatga aaaaaggcta tgcagaggtg 180 agtggttcat atgtcggatt ctcaaccgaa tagagtttta catcagggct ttgctgacac 240 ttgtcctagt accgcaatcg cctattcaag atcccgacat ctaataagcc gatggtaata 300 gtgccgactc acttactgga cgatctcaag gcgtaccctg aggacaccat cagttttcgt 360 cgggaaatgt acgaccggta cttggggcag tacacatccg tcgcttctaa cagcagtgcc 420 atgatccact cagtcaagtt tgatctgacc aaaaacattg ggaatctcat tcccctgatg 480 caggaggaaa tcagctacgc tatggaccat ttcatgtcgg ggtacacgcc tgcagaagga 540 tggaccaggg taccggttta cgccttgtcc acgcgtgtta ttgcgatggt gtctggcagg 600 gtgtttgttg gcttgccact gtcgcggaat gaggaatggt gagtatacct taatcttata 660 gagaacgatg taccttacct gatgctttga caggattcag accgcgattc attcaaccat 720 cgactctttt gttggagcgg ctatgatgtg gcagtacccc tcatggtctt ggcccatcat 780 gcaatggatc gtaccccaga caaaccgggt gcgatcctac cgtcggaggg ttgcagagat 840 gctcgagcca attattgcag gtaggctcga cgatatgaag aaaggatccg accggaaaac 900 gccttcggat atgattcagt ggctgattga caactccaat ggccgtggcg gtgacctcgc 960 attccaggcc aacgagcaca tcgtgatgaa cgtcgccgcc atccacacaa ccggtggtca 1020 gcttgcacat accctctatg atcttgcgcg acatcctgag tacttacctc agcttcggga 1080 agagatcgat tcggttttgt cccaggacgg cagcgttacc aagcagggcc tcttccgcct 1140 gaaaaagatg gatagcttcc tacgtgaagt gcaacggctg agccccccat ccatggtttc 1200 cacaaaccga aaagccctga agactttga actctccaac ggagtgatca ttcccgctgg cacatcgctg gctgcttctc cggcatgcgt cagcagggat cctgctatct ggaagaatcc agatgagttc gatgggcttc gattcttcaa actccgcgac tcagagggag aggagaagta 1380. ccaatttgca actgtcaatg acgacgcttt aagctttggt aagtgatagt cgatccagga tgggtgaatt tagatgacgc taatattggt ccaggtcatg ggcgtcacgc ttgtccagga cgctttttcg cctctgctga gctgaaggtc gtcttgatcc acattctccg aaactatgat atcggcttat gtaccgacta tagcaaggat cgacctctga accgctttgt agaggttatg gctgcgcagg atgtcacgta tgagcttcgt tttcgcaggc gagaatag <210> 18 <211> 1198 <212> DNA <213> Artificial Sequence <400> 18 atggcccctc acgctgagat tgacgtcgac catgtggtcc gaggatcgga ggcgaatagt caagatgcag acctagtcac gatctcgatc ggcaaaataa tggactgcga taaatcggcc gaggagagtc tgctgaatgc ctcgaaagat ctgggatttt tctaccttga cgtgagagat 180 catccttctc gtgaggtgac gaaccagatc gagattgcca catccgcagc gctgcaattc 240 tacgacctgc ctcaaagcga gaagtccatc tgggaggtca acaaagacca tatctccggg 300 gaggagatta tcggcgggtg agaaaacata gaacgaaacg taagccttga ttaattcaag 360 agggctaacg caactgatgc atagatacaa gcctgccgga attcagactg ggtctgtagc 420 aggcaaaggt gatggatttg aaggcttcct ggtaagttct actgctatct tccggaggcg 480 acgtcaccgt ggtatcctaa cctaaccttt gctgcagatt ttcgagcacg ccttgatgaa 540 tgctgctcgt gacaagcccg cgaatctccc gaaggcattc caggagagac gtgaggtgtt 600 tgaagctacg aatcagacac tggagagatat tggaagggtt cttctggaca gcctgtcgcg 660 atccctcgat ctgaacggca agcagaacct tgccgagcac caccgtaaag gccatgcctc 720 tcctaccgcg ctcggacttc tcaagtacct accttacgaa ccacaaagcg aggatgtcgg 780 ccatgtctcc cacactgaca ttggaagtct ttccatggtg ttctccgatg tgggcgggct 840 tcaggtgtat cacccaggaa agaggtcttg gatgttcatc ccacccaagc cagggcatac 900 cgtgtgcaac attggtgatt cagccgagtt cttgtccaag aatgtgctgc ggtcgtctct 960 gcatcgcgtc atcccacacc cagcggagaa aggcaagagc aagctgaccg tggtatatct 1020 aatgagacct gagacagata cggtcttcgt tgaccgcgag ggcaaggagt ggagaagtgt 1080 ggactggcac aacatgaaga atcgtttgtt tgcggaggac ttggagtctc aggctagcaa 1140 cctcgccctg actggccgtc tgggccacga cgatctttgg gacgagaagg atgtctga 1198 <210> 19 <211> 1626 <212> DNA <213> Artificial Sequence <400> 19 atgtatccca tcttaaccgc ggccgttgta ttggcgttgt acctcatcgg acttgctatc 60 tataggctgt accttcaccc gctggcaaag tttcccggtc cgaaaatcgc agcacttacg 120 gttttttacg agttctactt cgacggtatc aagcgaggac gttatacgtt tgagatccaa 180 cggatgcatg agaagtacgg tcccatcgtc cgtatcagcc ctgacgagct ccatgtcaat 240 gaaccctcct tcattgacga gctttacgcc gggagtggaa aaaagcgaga caaatacccg 300 tatttcagcg ctcagttcgg aatcccggac agtgtattcg gaacacccag ccatgacctc 360 catcgcctc gacgcggtgc cctcaaccgc ttcttctcca aatcatcggt caataaactg 420 gagccagtca ttcataatgc tattgaaaag ctggtctcgc agctccggac atacgctggg 480 agtggaaagc ccgtggctat caacatggca ttcagctgcg taacgacaga cgttgtcacg 540 gagtatgcct ttgcgaagag ttacaacttt ttgggatctt caacttttga gccaaacttt 600 caccgcgcca ttacgccgg aacggacatg ggtccatggt ttaagcagtt cccgtggctc 660 attaccctga tgaacagact tcctcagagt ctcgtcatgc ggatcaatcc tgaagccgcg 720 atttacgtgc ggtttcagga agatatcaga cgtcagattc gacagactca aaatgacatt 780 gctttggga agatcgacga tcagtctggg agaacaatat tccatgagct actgacaggc 840 gacctcccag agcaggagaa gagattggag cgtctgtggc aagagggcca gatcgtaatc 900 ggtgccggga ccgagaccac agcatgggca ctttcagcga tgctgttcta catcctcgat 960 gactccgaga ttcttagaaa gctgagagat gagttggagg aggccattcc cgatgccgat 1020 gagagagtca gctggaccg ttgacaa ttaccgtac tcagcgccgt ttactgcgag 1080 gggctgcgcc tttcctacgg tgtaagcacg cggctacagc gcatcaatcc ggtcgggccc 1140 cttgtttga agtccgagct ctctcttgag aagggcgaga aacgcgtcat ggccgactac 1200 gagataccag caggacacc cgtgggaatg accgcgactc ttattcaca ccatccggag 1260 ttattcccaa atcctgagca atttaggcca gagcgctggc tggatgagca agggcggcgt 1320 catcatcatc tgacaata tgttctgtca tttctcgtg gcagccgaca gtgtatcgga 1380 atcaagtaag ttttacgtgc tttgcatcg ggactgacac ccaatcgct gatattca 1440 tgcagcctcg cgtacgctga gctttacatg gtatttggc tgctcatccg ccgtatgggc 1500 tccagcatgg cccctatgg aacggcagg gagacgtcg agatacacta tgacaggttc 1560 gtcccacac cgaagacgg aactagggg gtccgagtcg tcatccagtc tacagcagcc 1620 mid 1626 <210> 20 <211> 3164 <212> DNA <213> Artificial Sequence <400> 20 atggctgtta cggacgcgac agacctttgt cgaaccgaca acgacaataa cgaacctctg 60 ctgaagacca gcgttcttga cctcttcgat cagcatgcga ggcgcagtcc gaatagcacc 120 gccgcagagt tccagggtca gaccatcact tacgatggat tgtacaatgc ttccatccaa 180 gtcgctttgg aattgcgtaa acgaggattc cgtcctcgcg atcgcgttcc cctgattacc 240 agcatgggct tggagatggt cgcctcggtc ttgggaattc tgcggctagg agcttcttac 300 tgtcccatag actttcaagc ttggagctta gaacgagttg ttgccacttt ggaagccgtg 360 ggctcacgcc tagttttcag caccgtggac acaacgataa ctggctacga gctggttcgt 420 gtgcctgaaa tgctgaggaa gaacaacccg gtgaccgacg aagcaacaca acaactaaaa 480 ctgatcaggc aagatatcag gaaatctgat ctcatttaca tcatcttcac ttctggcact 540 acgggaaagc cgaagggagt gatggtcccg cacagcagtg ctgcccacct tgtgcagcaa 600 agcttccccg gagcaatgag aggaagccca ggggagaagg ttttgctatt cttctcagtt 660 gcctttgatg gtcagtcact tttctattct ttaatcggag gtctctcagc agcgaactgt 720 ttagtctaac acttgccact cctacaggat gcgcaggagt catcttttcg acaatttgcc 780 atggtggaac tcttgcgatg gctcaaccgt ccgacgtcat caacacagcc tcttcatgca 840 gcactctcat tctgacacct tcaatgctgg ccagtctgga acccacccct gagttcgacc 900 gtgtgcacag catttacatg ggaggtgaag ctcccactga agctctggtc agactttgga 960 ccagtccgac aagaaaggtt tacaattgct acggacccac tgagtgcacc acggccgtct 1020 ctaccgtaga gatggttcct gatggcccga tcgttctcgg ccatctggtc tcggaggtgg 1080 agctggtgct gctcgatgag aatctagaac atgaagttcc gcagggagag atctgcatca 1140 gagggccatg cttggcggtt gggtacctca atatgggggc tctcacacag gaaaagttct 1200 tcttcagaga cggcatccga cattaccgta cgggagacct tgcccgccgt gacgaggaag 1260 gtctgcattt tgtggctcgg gttgaccgca tagtcaaaaa tcgcggattt ctgatcaact 1320 tggaagcgga agtcgaaccg gctcttcgca gcttttcagg agtccagaaa gccgcggcat 1380 tccttctgca gaagcagctg gtggccttcg tgacgccttc tgaggcaccg gtcgagacac 1440 tgcgcgggca cctcgctaaa cgattcgatg cttttatcat acccgacctc attttcgccg 1500 ttgatgactt cccgttgacc acaaacggca aggtcgatac aagagccttg caggcaatgg 1560 ccacgcatca agttgatgaa cacctacgca gttccggaga cagtgaggcc gacaaatctc 1620 ctgccttgga cgttgttctg gaagcctttg cagatatctt caaccgacca gcgagccaga 1680 taccgcccac agcgtcgttc agactcctcg gcggcaactc ccttacggct gtgaaacttc 1740 agtctcatct tcgccgcgca aaccttagtt tgatgttgag caagatgttc gaattggata 1800 cggcagcagc gatagctcgg gcggcagtct caatggacta caacattgac cgtgtcagct 1860 cgaccaacgt gcatccatca actacgccac ttacgccgca tcaaatcgag cttttgaggg 1920 agaccgagat tgactcatcg agcaattaca acttctactg cctcactaga atcatcggtc 1980 acagccccgt caatactgaa cgcttgcgag aggcatggga ggtgatattc gcccgtcact 2040 ccatataccg aaccacattc gacatggaca cgaaaaccca gacagtgcac gccgggcgag 2100 acatcaactg gcgggagttc acggcgccag actatgaaca gatgaaggcc gtttctgagc 2160 gggagagaga ggtgttgtgg acaatactac gttcccaaaa gccacggata cccattctga 2220 agcctcattt ctggatcgtt gaagtgcctg gagaggccat tcagatgaat tggcttgttc 2280 accacgtcta tactgatgcg tggtctttcg caattctgtt ggaagaactc gaacttatca 2340 tcaatggcaa tgctgccaca cttcaacctg cacctggctt cgaactggta gcccagcact 2400 tggacaaaga agctcggtta caagcaacag ctatacggga tttctggagt aggtacgcga 2460 cgccgtggac agagctgaga ccgattcaac tacccgtccc cagggagccc agtatccagc 2520 catgggacaa gtgggagtcc gatatccctt taaaagtgga gagccttgaa ctgtttgccc 2580 gccagcatgc agtctcttcg gcgacagtga tctacactgc gtgggcctta gtcctcactg 2640 attacaccgg ttctggtact gtgggcatga aggtcagtgt ctccgggaga aatatcgacc 2700 atccggctgt cgagcgcgtc atgggatctc tcaatggccg ttgtccgctc ctagtaccga 2760 ccagtaaaga cgcaacgatt ggggaaacac tgagatcgct gcagagtacc tttctccgaa 2820 tcaatgacta tcagtggtcg tacccggagt tgaaaagaca tgtagccgag gtatttggag 2880 accaagccta ctggtttgat tcccaagtag ttgtgttatt agatacgccc gtagacacaa 2940 gtcattgggg cattttcgag gtgcagaagc cgactgctcc catctggctt ggtgtcattc 3000 aaggcgggga cgtgttcaac atgcgtttgc gatacgatgg cagttgttat gcacaggata 3060 gtgttcagag catgggcagg agattcatcg acagactgat ccagttggtt gattctcccc 3120 tcgacaggag aatcggggat ctaggtcaca tcacgcaaga ctga 3164 <210> 21 <211> 1065 <212> DNA <213> Artificial Sequence <400> 21 atggccgtcg tcactgtgtc agaccctact tttcgggcgt acaaaggcac tcaagccaag 60 cgatacgacg aaggccgccc cgcgtacaca ccggcgcttt acgaggccat actgaaatat 120 catgatgata atggcggcca ccggggcaca gtcatcgacg ttgggtgcgg ccctggaaga 180 gcaacgcggg aattggcacg tttcttcgac aatacgatcg gcgttgaccc cagtgaaaat 240 atgattgaca ccgccaagca agttggtggt gccgggaaca ctggcaagcc gattgagtac 300 tacgtttctc ccgctgagaa aattgcggac ctggaagaat tgccagatgg caaagtcgat 360 ctcttgacag ccgcccgccg ggttagtgag ccctgtaatt tcaaacccat gccctgggaa 420 ttgctaactc tccgaacagg cgcattggtt tgacatgccc aagttctggg gccaagctgc 480 ccgtctcttg cgaccaggag ggtcagtagc tctatggacc actggctgtt tctatagtcg 540 tatgtgcctc aataggagaa aatcccgctt cagggcatca atgaaaagat cgatggctaa 600 ctggatgtat tctgagcaga cccagcgtct gacaacgcag gggaattaaa tcgaattatg 660 cgccagctgg aaactgacga gctcgagcaa tttgagctga cccctaacgt gctgtctcgc 720 accctttacg acgacctagc tcttccatgg aaacttgagt ctcctcagcc cgactttgag 780 gagtcggcct atgttaggcg agagtgggat cgcgacggtg ttcttaccga cggggaggac 840 ttttttggag actcgctgga aatctcgtta gacatgttgg agaagggtta cgacacggct 900 agcactgtca cacgctggcg ggcagctaac ccggagctag cttatggccc tcatgattgt 960 gtcaagagga ccattgcaca gctccgtaag gccgtgggac ctgaatcgaa cggattgatt 1020 cggggaagta cttcgacagt tcttctgctc tttaagcgtc gctaa 1065 <210> 22 <211> 1992 <212> DNA <213> Artificial Sequence <400> 22 atgattgacc aaatagtgtc ccacaaggag aatttatctc aaactctagc ggctgcggca 60 agggcgtctt cttcagacat tctcatttct gccttgctcc tggtgagtcg attcatcgcc 120 actgatgtag cataagcgca gcggctgaac caggcaccag ctatgcttct acatcttgac 180 tgttggggtc tacaatgtct atttccacaa actttccacg tttcctgggc cgcttttggc 240 aagatgcagc ttggtaagag acccctgagc tctgcgccct cagcgatgtt gagattgact 300 ttgaggtaga tatggcgcat ctaccattcg cagactggtc gctatcattg tgctatcgag 360 gcgcagcacc gtaaatacgg taagcgcttg ggggcttcct tgaattgaac gcattgctga 420 agccacgtag ggcctgtcgt cagagtgtcg cccaacgaac tgtcctttgc gaccgttgag 480 tcgtggaaag ccatctacgg gcatcgtgtt ggcggcgcac ccacacccat caagagcgag 540 ttctacgaca tatatggggc tggcttcaag tccctgtgta tcggaagcga gcgtgatccg 600 cataagcatg gtcagatgcg caagatgctt tccgccgcct tctccacgag ggtgttgaca 660 gaaagagg acattatcac caaagcagtt gaccaattcg ttgttcgact tggcacagat 720 ggagcgcagg ctaacggtct caacatgacc aaatggtacg aaatggttgc ttttgattcc 780 ttgggcgaaa tggcttttgg agagtcgttt cacagcgttg aggctggtat gtttctaaca 840 agcaaatttg gtcttgttgt tttactcact tttccgttct tcaggtaaac cacatagctg 900 gtcggagatc atagtcagcc atctctactt cattaccttg attgacaacc tgcgccgtct 960 ccctttcgtc gccaggctgg ctaggctgct gtttccatca acgctagcgg tgcaaaacca 1020 gaattctcag tacagccgca aaaaggttga acagtgagtc ggacacgaca aattctacaa 1080 tttgtcagac taatcgcatg agacaggcgt ttatctaaaa gctctacccg gaatgacttc 1140 1200 cacgtttcta cgttgacgta tgtataccga cccaaacgcc gttatatgat agaagttgct 1260 agtgtgctaa ctcaaatgca gcattgctgg cggagagacc actgcgacat tccttgctgc 1320 ggcgacatat tatttgctgc gcaaccctgc ttgtctcact tatgttcagg aagaaatcag 1380 ggccaagttt tcttcgtatg aggcgattaa tgccacagca gctagacaac ttccttacct 1440 gcaagctgta attagcgaag ggctcaggat gtacgcgccc ggcagccaag gcttccccag 1500 attgtccaca ggcatgaaag taggagatgt ctatgtccca gccggcgtaa gcacagccct 1560 ctgagcatac cccgcctact gtactgatgt ggctcttagg ctgaggtgtt tacacacgcc 1620 tggacgttga ctcatagcga gaagtacttc tctgatccga aagagtttaa acccgaacgc 1680 tggattgatc cccagtccac cgatgtgaaa gatgctagcc agccgttctc catgggacca 1740 agaggttgct taggccagaa gtaagttctg acgagtcgcg cacttagctg ctagtttacg 1800 acaaggattt ggagctgaca tgtgcccagc tttgcatata tggagatcaa cttgattctg 1860 tccaagatgc tgtggaaata tgatctggag cttgtgaaca agaatttgga ctgggagcgg 1920 gagagtcatt tgcatgtgat gtggtggaaa cccgcattga tggttcggtt caaggagaga 1980 aggtctccat ga 1992 <210> 23 <211> 1169 <212> DNA <213> Artificial Sequence <400> 23 atgcctccat ctatcttgaa cgattatgat gagactcccg tcttcatcgg caaagtagcc 60 gatgttcagg agcaacctgc cagcaccgca acggcagtgt ctctccccat cgcccctcac 120 ttcaccccgc caccgtctcg tttcgtggcg aaatgtcatc ctttggagaa gcaaatcaca 180 gctgaagtcg atggctactt tctcacccac tggcctttca agacagccaa ggacagaaag 240 aagtttgtcg ctgcaggctt ctcgcgtgtc acatgccttt actttccttt gggcttagat 300 gatcgcatcc atctcgcctg taggcttttg acaatcctgt tcttgattga tggtacgttg 360 acccccgaac aatccgttga ttacatgtct gattttgaat atccgtagat atactggagg 420 acatgtctct ggaagacgga tgtgcttaca acgagaagct catgcccatc gcccggggtg 480 atgtccagcc ggaccgcaat gttcctgtgg agtggatcat gtacgatcta tgggaaacca 540 tgagatctca tgataggcaa ctggccgacg atgtgctgga gccgacattt actttcatgc 600 gtgctcagac cgacaaggct agattgtcga tccgtggcat gggcccctac ctgatctaca 660 gagagaagga tgtcggaaag gcgtaagtac aaagcctgtg tggaatgata tggaggcatg 720 gcattaatca tccccgtacc agactactct gctctctcat gaggttttcg atgggtctcc 780 gcgtgtcacc cgaagagctc gcatcagttc aacccatcga acaaaacgtc gccaagcatc 840 tctctgtcct caacgatatt ttgagctggg acaaggagtt tctggctgcg cagacgggtc 900 accccgaagg atcggcgctt tgctcggcgg tacaggtttt ggcagatgag acgtctctca 960 catacgcagc ggcgaagaga gtgctctggg tcatgtgccg cgagtgggag tatgagcacg 1020 actatcttgt tgagaagcgc agacaagatg gggagctccc gcctagcgag aatctgatgc 1080 agtacatcaa gggattggag taccaaatta gcggaaacga gatttggagc aagaccacgc 1140 tgaggtacaa cgctcccaca accgcttga 1169 <210> 24 <211> 528 <212> DNA <213> Artificial Sequence <400> 24 atgacatccc tcaacttcac tctagaagac atccctactt tgcacaggaa gcgggtcatc 60 Met-Thr-Ile-Pro Ser-Thr-Phe-His Ser-Arg-Glu-Thr-Pro-Tyr-Phe Cys-His-Glu-Ala-Gly-Ser-Ile 60 atcaccggcg catcatcagg catcggactt gctgcagccc acatatttgc acagaaagga 120 Ile-Thr-Gly-Ala Ile-Ile-Arg Ile-Gly-Thr Leu-Leu-Gln-Pro Thr-Tyr-Phe-Cys-His-Glu-Arg 120 gcatcggtcc ttaacctcga cataaacccg ccaccaggcg atcaaccaga cggcatcgag 180 Ala-Ile-Gly-Ser Leu-Asn-Leu-Asp Ile-Lys-Pro-Ala Pro-Thr-Gly Ile-Asn-Arg Asp-Ala-Ile-Glu 180 tttcgccagt gcgatatttc aagctggtcc agccttctag gtgcctttcg atatgccggc 240 Phe-Ala-Ser Ala-Asp-Phe Gln-Leu-Val-Gln Pro-Phe-Ser Val-Pro-Phe Asp-Tyr-Ala-Gly 240 gatattgaca tcgccgtttc caacgcaggc gtctcggaag aggttgacta tttcgccgac 300 Asp-Ile-Asp Ile-Ala-Val-Phe Gln-Arg-Ala Ser-Ser-Glu-Glu Val-Asp-Tyr Phe-Ala-Asp 300 acgtttgacg aagttactgg ggacctgatc gagcccgagt atcgagtact cgacatcaac 360 Thr-Phe-Asp Glu-Val-Thr-Gly Asp-Leu-Ile Glu-Pro-Glu Tyr-Glu-Tyr Ser-Asp-Ile-Asn 360 ctccgcgcag ttgcaaactt tgcaaaactc tccgtcagcc atttccgtcg ccgaaagtgc 420 Leu-Arg-Arg-Gln Leu-Gln-Asn-Phe Gln-Asn-Ser Ser-Val-Ser Pro-Phe-Arg-Ser Glu-Ser-Cys 420 ccagggagca ttgtcatcac ctcaagtgcc acggcatatg cgcctgagca gtcacttccc 480 Pro-Arg-Glu-Ala Ile-Val-Ile-His Leu-Ser-Val Pro-Gly-Tyr Met-Ala-Leu-Ser Ser-Thr-Phe-Pro 480 gtatatagcg gatcgaagtt agctgtaggc tgccccttct ccgtatag 528 Val-Tyr-Ser-Ala Asp-Glu-Val Ser-Leu-Val-Ala Ala-Pro-Phe-Ser Val-Tyr 528 <210> 25<210> 25 <211> 954 <211> 954 <212> DNA <212> DNA <213> 人工序列(Artificial Sequence) <213> Artificial Sequence <400> 25 <400> 25 atggatacca taggattcat tcgtgctcag tggaaaaacc ttcccatcgt ccccacaaag 60 Met-Asp-Thr-Ile-Arg-Asp-Ile-His Ser-Val-Leu-Ser Trp-Lys-Asn-Leu-Pro-Ile-Val Pro-His-Lys 60 cagacctgta ccggcaaaac ctacatcatc acagggtcaa actctggcat tggctttgaa 120 gctgtcaagc accttgttaa tcttggatca tctcgtgtca tcatcgccgt cagatccatt 180 gagaaaggtc aagaggcaaa agcccgtatt gatgcctcca ctggcagacc gaagatcacc 240 gaggttggc agcttgacat gtcatcctat gaatccatcc gagcctttgc taaaagagca 300 tcgtcggagc tcagcgtat tgacggcctg gtcgaaaacg ccactgcagc tttggataga 360 tggacagtat ctgaaggcat ggagacaagc gtcactgtca acgtcatcgg cacagtgatg 420 ctagcactgc ttcttcatcc gaagttgatg gaaacggcca agatttcaga ctcgcagccc 480 catatcgtgg ttgtcacctc tggacttggt ttcacgcagc cggacttttt ggatcacgca 540 gataaggatg tctttgatgt cttgaacact caaggggagt ctcccatgga gaaaaggtac 600 gtcacaagcc ttctgactat catgtacacg gcttgctgac tgaacgactg cagatactcc 660 gttacaaagc ttgttcagat acttgccatc cgacaactcg cgaccatgct accagcatct 720 cgtaccggcg ttgtcttgaa cctcctcaat cccggtcttt gcaacaccgg cctagcaaag 780 tatggcagaa tttccctacg gcttcaggtt cggatgttca acgccctgat tggaagaact 840 ccggagatgg ggagccgcac gatcttgcat agcatgtttg ctgggccaga gagccatggt 900 caatatgtct cagattgtga gattaaagag tacgtctaca tcatcaagca ctga 954 <210> 26 <211> 1499 <212> DNA <213> Artificial Sequence <400> 26 tgagtgaagt tccggtcgga gcaatcaggg tcggttggag tatatcagaa atgtatatct 60 ttggaaaggg tagttataat atgtcgaata tgaatttagg taagagtatt tgaaacaaca 120 agtatgagga tggttcactg gatggcttgc cctaacatcc acaccatcgc atccggccac 180 ctcaggaaca atcactcctc ctcacaaggt atatcccgcc attgaccaac gacaatccag 240 agtatgagat acttgcagaa cgccgagtgg cgttatcaac cgcgtgacct attcagtacc 300 tttgcctcac gccaagcaaa tttccccaca caaaagcaac cataaatgtc gttggccacc 360 gaagctcaaa ctttcggagt cgctctcagt ggttacttta ctgctggtgc ccacgggatc 420 gtaaatggac cttgaccaat tcgagtagtt ccgcagacga tcgctcatcg ctcgatttgg 480 tcgcgtgtcc agagtgttgc tacagcatgg tctggattcc aatccacgca gcagatatct 540 cttttacgca gtaactattc gatagccatt tccgttcaga tcagccgtcg gatccgagga 600 gcgacgacat caatgcgtgt tattagtcaa atttggagg ggttgcgtgc ccactgcagg 660 cagatgtagc cgtggcacca caacaccggc cagccctgga ttgggtggtg gaaccaagat 720 atgagaacca gtatctattg gcacggaggc gtttccggag cctgccgcgt gtgatacgtg 780 cagagtcaat tcctacggac tgtctggggt aggatcacaa ctaatcagtt gcagcgatgg 840 tttgacaaca ggggtcgatc aaagtttccg aagaatagga agcgaggaca gcaccaaggc 900 cgctgaacca caggaaacaa aggaaggaaa aacacaacaa aaccaaagac agacacatag 960 gaaatgacat acttaccaga gataagatga aaagcaccat ggggagggag ggtatggatg 1020 gggaagttga tcaacagtct gaaacccgcc cgaaatgaat gcatgacgcg acgattccat 1080 ctccacctaa gcttcatccc gtcaacctct aaacgcgc tcggaggaga aaaagagggg 1140 gatcaacagc aatctaggtt gttgttcccc caggatttct ccgcaaagaa tgtttagggt 1200 tccgtggatc gggtgaccga atcggccaac cgcattgtct gatcgtctcg tcatatgatc 1260 cagtgcatga cgtcttccat caatccatca ccccagactg aatcctcaca ttaatttaac 1320 aattgtcgct cggggaaatc aataaatacc cgctcgtctc ctccctccct cgtgctggtt 1380 cagttgattg ttcactcatc gactttatca atcttccatt gaccattcca ggcttgtcgc 1440 ccactcatat aatcttcttt ccccggtctc acatcaatca cccttcacac cacaacacc 1499 <210> 27 <211> 604 <212> DNA <213> Artificial Sequence <400> 27 tcatggtgtt ttgatcattt taaattttta tatggcgggt ggtgggcaac tcgcttgcgc 60 gggcaactcg cttaccgatt acgttagggc tgatatttac gtaaaaatcg tcaagggatg 120 caagaccaaa gtagtaaaac cccggagtca acagcatcca agcccaagtc cttcacggag 180 aaaccccagc gtccacatca cgagcgaagg accacytcta ggcatcggac gcaccatcca 240 attagaagca gcaaagcgaa acagcccaag aaaaaggtcg gcccgtcggc cttttctgca 300 acgctgatca cgggcagcga tccaaccaac accctccaga gtgactaggg gcggaaattt 360 aaagggatta atttccactc aaccacaaat cacagtcgtc cccggtattg tcctgcagaa 420 tgcaatttaa actcttctgc gaatcgcttg gattccccgc ccctggccgt agagcttaaa 480 gtatgtccct tgtcgatgcg atgtatcaca acatataaat actagcaagg gatgccatgc 540 ttggaggata gcaaccgaca acatcacatc aagctctccc ttctctgaac aataaacccc 600 acag 604 <210> 28 <211> 745 <212> DNA <213> Artificial Sequence <400> 28 ggatccgaac tccaaccggg gggagtatat tgagtggccg cagtggaagg aatcgcggca 60 gttgatgaat ttcggagcga acgacgccag tctccttacg gatgatttcc gcaacgggac 120 atatgagttc atcctgcaga ataccgcggc gttccacatc tgatgccatt ggcggagggg 180 tccggacggt caggaactta gccttatgag attaatgatg gacgtgtctg gcctcggaaa 240 aggatatatg gggatcataa tagtactagc catattaatg aagggtatat accacgcgtt 300 ggacctgcgt tatagcttcc cgttagttat agtaccatcg ttataccagc caatcaagtc 360 accacgcacg accggggacg gcgaatcccc gggaattgaa agaaattgca tcccaggcca 420 gtgaggccag cgattggcca catctccaag gcacagggcc attctgcagc gctggtggat 480 tcatcgcaat ttcccccggc ccggcccgac accgctatag gctggttctc ccacaccatc 540 ggagattcgt cgcctaatgt ctcgtccgtt cacaagctga agagcttgaa gtggcgagat 600 gcctctgcag gaattcaagc tagatgctaa gcgatattgc atggcaattt gtgttgatgc 660 atgtgcttct tccttcagct tcccctcgtg cagatgaggt ttggctataa attgaagtgg 720 ttggtcgggg ttccgtgagg ggctg 745 <210> 29 <211> 567 <212> DNA <213> Artificial Sequence <400> 29 ttgtattggt attaccagaa cgtcacgaga ccgcctgccg caatgttctc atcctttctt 60 tgctactagc aagttttttc gcaatttctc gaagctttag atggtctttt cttttccagt 120 cgaaagctac tagccggtct tttctgtcct attcagctta ggcagttata ggatatcttc 180 aagattcagg tactctttga ctacacacaa tgccctatga tatgaagggt aaatgtgtgg 240 gtatcattca ttggatctta agtaaggcac agcccgcgga gcagaaatga agctccttgt 300 atgacatcac caaatggtca cttaatgcaa tttcgaaccc tttccatccg agctcaagtt 360 cgagagctca gttcccattt tactcatctt ttttacttag aagagggata taatctaata 420 acaaactgat ttaaatgaac acagagctat tactttcaaa tttggcttaa tgtccacttc 480 tcgccaccca acggtcctat ggggtagtgg ttatcccacc ggattttgat gaacaatata 540 catgtcctgt atatgtgtag gaagatc 567 <210> 30 <211> 182 <212> DNA <213> Artificial Sequence <400> 30 gatctgtagt agctcgtgaa gggtggagag tatatgatgg tactgctatt caatctggca 60 ttggacagtg agtttgagtt tgatgtacag ttggagtcgt tactgctgtc atccccttat 120 actcttcgat tgtttttcga accctaacgc caagcacgct agtctattat aggaaaggat 180 cc 182 <210> 31 <211> 269 <212> DNA <213> Artificial Sequence <400> 31 actagacggg ttcgcatagg tttggggttg tatcttggcg ttgggacgga ctgggtatgg 60 tgtttctttt ggatatatga catgatatgt acacggccgt gaatctttaa ctttatatca 120 ttatagaaat gcacttgcac atttcaacac gctgcgagca gaatctcgaa gattgttccg 180 caagtattag atcatgagag cattttcatt tcctttcagg cagtgggagt aggccatcct 240 gaaaacaagg cggccactgt agactagag 269 <210> 32 <211> 8546 <212> DNA <213> Artificial Sequence <400> 32 tggggatcct ctagactgat gatgcagtgg ttcgttcaca ttctgctctt gaagattgga 60 gtcgctaaaa tgaataggag ttgagctctt gcttcgggtg tggtggcggg cgcgtgagcc 120 actccgggaa ggcgcacgag agaggacgcc gggtgtccct gggaccgaat agctagacaa 180 gtaggaagtt ctagtggggt tgtcaatagc ctcatttcgc ggggtgaaat aatcctgtgg 240 gtctttgtcc gactcattgt cgtcgtcgtc aatggggtaa tggggagtta aaggcgcgag 300 tgagatgcgg tttaaggatg gataagagcg gcggggccga gcttgtgatg ccgaggactg 360 ttcagctttg ttagcgaatc agcaggattg tggatgtagt aaagtcgacc tacagccatt 420 gcgaaacctc agaagaatgt gctggataga ggtcaagagt atggaatgag gagagtcgag 480 ggttggggtg tgttaggcct ggatctacct tggctaggac gactccgcat tcgtgacatg 540 tgaaagtcac cgggtgcgat ttgccccatt tccaatactc tgagtaatat aaggaagcga 600 gagaacatgt caattagtta tttgatatat gctagaaaga attggtcagg ataattggga 660 aaatgcttct actaacaggc ttttacgtga tcggagtaaa tatatttagg gacggaatga 720 tctcataccc gtcaactcat cagtttgata acgccagcct ctatatattt aaatggaagc 780 ctggttatat ttattcaatt tttcccttat tgacaacgaa accctccaag gcggaaatcg 840 cagcccataa ccggttctca gcctcaggga agacaagtga ccggttgctg tagaaaacct 900 cgtcgctgac ctcctcaggg tgtcgcggga gacagtgcat gaacttccag ccctccttag 960 ctcctcctcg cttggcgagt tcagcagtga tttggaaacc atcaaactcc ttcagcctct 1020 gagccttctc ttcctcttgg cccatagaga cccaggtgtc tgtgaccaga atatcggcgt 1080 ccttgaccgc ttcttcggga atattggttt gcagaagctt tcctggtttc gagacaccct 1140 ctccagcaga cttgatgagc tccagcatgt gaggagggat ttcgtacccc ttgggagtag 1200 cgacagcaat gtcgacaccc atttttgtag cagcaatggc catatcgaac aggacgttgt 1260 tggcgtcacc cacccaagcg atcttcaatc cttccaaccc tagacttgaa aggtggtgcg 1320 ccttgggggt gaatgcttca tagatggtct ggaaatcggc cacggcttgg agagggtgga 1380 aagagtcaca caaagcattg atgactggaa ccgaagagtg cttcgccaga tctgcgacct 1440 ctgcatgttt accgacacgg gctacaatgc aggataccat ggacgaaatg acaacggagg 1500 tgtcgtatag ggactcgttg acacctagtt ggatatcatc cttgcccaag aacatcggat 1560 gacctcccat ctgcacaacg gccccttctg tagataccct tgtcctcgtg cttcgtttgc 1620 tgaagatcat ggccacagtt ttcccaagga gtgatccctg caagttttgg ggcattgacc 1680 ccgacttgat agcccgtttg tgtgaggagg cattgcgaac aagggttgcg aactcggagg 1740 gcgagaggtc cgcaatggag aggaagtggc ggggagcaaa gggtgaggcg ggaggcatgg 1800 tttgcgagga gtaggatcgc acggtgtgtc gcaagctctg gctcttgagt acggagcgaa 1860 gggatgccat tggaagtgca agaatataga tgttaaagaa cttaaaagac ttggggcgga 1920 aagaaattgt ggacagaacg agggcatcca caaataactg agaaatgatt cgtgaatgac 1980 tcataagtcc gccagcagga gcttccgatc attggtaaag aaacagagtt agcgcccctt 2040 ccgttcgctg atcacggatc accgccattg catcaggccc ttgactctgc tgataccttg 2100 aactgctcac agtccttcat cgtcctacct ctcgcttcat caatatttgc tgcaattatt 2160 gcatgcgttc atctctattt atctaattta tgtaaattat agtcaaatga ggcctctaaa 2220 ctggtcatat ctggcccgat tcgccgtctg atcagcctca acactatcgc atactctcca 2280 caatccacac tttccccccc actccgcttt acacagcgtt cgttatgacg gatgaggcga 2340 gtcggcctct gttgggcgag catcacgaca ataatgcttc tccctatcaa gcgtcgtcta 2400 caaatcaaca gcgtcgctcc tttgaagtgt cttcggagtc aaccccactt ctccatcgcc 2460 gcgatgatga tatatcggcg tacggtggta tagagtcatc gcgggcttcc tctgctgcgt 2520 ccgggtctgt ttcattagat gactacccca aaaaaccgcg aaataaagct tggtggacgg 2580 tattatgcgg cctccttgct gtcggttccg tcattgtcat cctcattctg gccttctttg 2640 ttcccgaggt agtaaagcaa tacgtcaagg aagcagccat atttagaccg acaaacctat 2700 cagtcgtgtc ggccacaagc gaaggtgtca gggcaagggt acaaggggat attgtcctcg 2760 atgctgatcg agtcaaaagt gaatcggcga gaaatattgg gcgtttcgtc acctggattg 2820 ggaaagaggt cgaaaccggc cagtctgaag taattgtacg cttaccggaa tacgaaaatg 2880 cccttgttgg atctgcatcc ttgccttcca tcaaggttag cattcgcaac ggccatgtga 2940 atcaccttga cttcgaagcc gatttgattg ctggcgacat tgagggcttg cgctccgtcg 3000 ccgtcgactg gctggaaggg agactggacc gtctattact tcatggaagt gtcattctcc 3060 atatcaaatc aggcctgctg agcctgggag agcagactct agagtcgacc tgcaggcatg 3120 caagcttgc actggccgtc gttttacac gtcgtgactg ggaaaaccct ggcgttaccc 3180 aacttaatcg ccttgcagca catcccctt tcgccagctg gcgtaatagc gaagaggccc 3240 gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc ctgatgcggt 3300 attttctcct tacgcatctg tgcggtattt cacaccgcat acgtcaagc aaccatagta 3360 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc 3420 tacacttgcc agcgccctag cgcccgctcc ttcgcttc ttcctct ttccgccac 3480 gttcgccggc ttccccgtc aagctctaaa tcggggctc cctttagggt tccgatttag 3540 tgctttacgg cacctcgacc ccaaaaaact tgatttgggt gatggttcac gtagtgggcc 3600 atcgccctga tagacggttttcgccttt gacgttggag tccacgttct ttaatagtgg 3660 actcttgttc caactggaa caacactcaa ccctatctcg ggctattctt ttgatttata 3720 agggattg ccgatttcgg cctattggtt aaaaatgag ctgatttaac aaaaatttaa 3780 cgcgaatttt aaaaaatat taacgtttac aattttgg tgcactca gtacaatctg 3840 ctctgatgcc gcatagttaa gccagccccg acacccgcca acacccgctg acgcgccctg 3900 acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg 3960 catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat 4020 acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac 4080 ttttcgggga aatgtgcatg attacgaatt catcacgtgc tataaaaata attataattt 4140 aaatttttta atataaatat ataaattaaa aatagaaagt aaaaaaaagaa attaaagaaa 4200 aaatagtttt tgttttccga agatgtaaaa gactctaggg ggatcgccaa caaatactac 4260 cttttatctt gctcttcctg ctctcaggta ttaatgccga attgtttcat cttgtctgtg 4320 tagaagacca cacacgaaaa tcctgtgatt ttacatttta cttatcgtta atcgaatgta 4380 tatctattta atctgctttt cttgtctaat aaatatatat gtaaagtacg ctttttgttg 4440 aaatttttta aacctttgtt tatttttttt tcttcattcc gtaactcttc taccttcttt 4500 atttactttc taaaatccaa atacaaaaca taaaaataaa taaacacaga gtaaattccc 4560 aaattattcc atcattaaaa gatacgaggc gcgtgtaagt tacgggtaat aactgatata attaattga agctctaatt tgtgagttta gtatacatgc atttacttat aatacagttt tttagttttg ctggccgcat cttctcaaat atgcttccca gcctgctttt ctgtaacgtt 4740 caccctctac cttagcatcc cttccctttg caaatagtcc tcttccaaca fathergt cagatcctgt agagaccaca tcatccacgg ttctatactg ttgacccaat gcgtctccct 4860 tgtcatctaa acccacaccg ggtgtcataa tcaaccaatc gtaaccttca tctcttccac ccatgtctct ttgagcaata aagccgate caaatcttt gtcgctcttc gcaatgtcaa cagtaccctt agtatattct ccagtagata gggagccctt gcatgacaat tctgctaaca tcaaaaggcc tctaggttcc tttgttactt cttctgccgc ctgcttcaaa ccgctaacaa tacctgggcc caccacaccg tgtgcattcg taatgtctgc ccattctgct attctgtata cacccgcaga gtactgcaat ttgactgtat taccaatgtc agcaaattt ctgtcttcga aggtaaaa attgtacttg gcggataatg cctttagcgg cttaactgtg ccctccatgg 5280 5340 caactaactc cagatattcc ttggtggtac gaacatccaa tgaagcacac aagtttgttt 5400 gcttttcgtg catgatatta aatagcttgg cagcaacagg actaggatga gtagcagcac 5460 gttccttata tgtagctttc gacatgattt atcttcgttt cctgcaggtt tttgttctgt 5520 gcagttgggt taagaatact gggcaatttc atgtttcttc aacactacat atgcgtatat 5580 ataccaatct aagtctgtgc tccttcttc gttcttcctt ctgttcggag attaccgaat 5640 5700 acgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag 5760 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca 5820 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc 5880 agaacgctg gtgaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat 5940 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc 6000 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg 6060 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc 6120 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat 6180 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga 6240 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc 6300 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc 6360 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt 6420 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc 6480 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc 6540 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca 6600 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca 6660 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt 6720 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta 6780 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg 6840 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc 6900 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag 6960 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa 7020 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc 7080 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc 7140 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta 7200 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag 7260 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct 7320 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga 7380 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc 7440 ggcctttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt 7500 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg 7560 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg 7620 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggttttcc 7680 cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc 7740 accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata 7800 acaatttcac acaggaaaca gctatgacca tgattacgaa ttaactgcct ttttggacca 7860 acctttagaa gacagagccc gcctgatact agatgtagtt gcgtattt ggaactcatc 7920 ccatagaaag atggccattt cgtcaaggta tagatcaggc ttttcaagaa gatgatcaca 7980 aagagcctcc agcattattg gtgagatgct tcgtgggcgt ccacctttat ttggaggggc 8040 tttgctactg ccgaatagcc ggagatttga ccgaatccta ggaattgcgc gtgtgctgca 8100 gttagcagct tcagctatct gggcattagt aagtgatgga tcgcacttga tcatatcaca 8160 aataatttga tgttgcgaag gcgccaggtt gggtgccatc atatccctgt ggtaagattg 8220 atcgtcagga gattatctgc aggaaacatc atggtggggt aaccaaggtt gtgtctgtat 8280 aatatataca tgtaagatac atgagcttcg gtgatataat acagaagtac catacagtac 8340 cgcgttatga aaacacatta atccggatcc tttcctataa tagactagcg tgcttggcgt 8400 tagggttcga aaaacaatcg aagagtataa ggggatgaca gcagtaacga ctccaactgt 8460 acatcaaact caaactcact gtccaatgcc agattgaata gcagtaccat catatactct 8520 ccacccttca cgagctacta cagatc 8546 <210> 33 <211> 4731 <212> DNA <213> Artificial Sequence <400> 33 tatcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac 60 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac 120 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc 180 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg 240 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc 300 actcaaaggc ggtaatacgg ttatccacag aatcaggga taacgcagga aagaacatgt 360 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc 420 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa 480 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc 540 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg 600 cgctttctca tagctcacgc ttaggttatc tcagttcggt gtaggtcgtt cgctccaagc 660 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc 720 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca 780 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact 840 acggctcac tagagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg 900 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt 960 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct 1020 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga 1080 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa 1140 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac 1200 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga 1260 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc 1320 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca 1380 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta 1440 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg 1500 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc 1560 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg 1620 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt 1680 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaccaagt 1740 cattctgaga atagtgtatg cggcgaccga gttgcttg cccggcgtca atacgggata 1800 ataccgcgcc actagcaga actttaaaag tgctcatcat tggaaacgt tctcggggc 1860 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac 1920 ccaactgatc ttcagcatct tttacttca ccagcgttc tggtgagca aaaacaggaa 1980 ggcaaaatgc cgcaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct 2040 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat 2100 ttgaatgtat ttagaaaat aaacaatag gggttccgcg ttcattcat cattttttt 2160 ttattcttt ttttgatttc gttttccttg aaattttt gattcggtaa tctccgaaca 2220 gaggaaa cgaaggaagg agcacagact tattgtagtgt 2280 tgagaaaca tgaaattgcc cagtattctt aacccaactg cacagaaaaaacctgcag 2340 gaaacgaga taaatcatgt cgaaagctac ataggaa cgtgctgcta ctcatcctag 2400 tcctgttgct gccaagctat ttaatcat gcacgaaag caacaact tgtgtgcttc 2460 attggatgtt cgtaccacca aggaattact ggagttagtt gaagcattag gtcccaaat 2520 ttgtttacta aaaacacatg tggatatctt gactgatttt tccatggagg gcacagttaa 2580 gccgctaaag gcattatccg ccaagtacaa ttttttactc ttcgagaca gaaaatttgc 2640 tgacattggt atacagtca aattgcagta ctctgcgggt gtatacagaa tagcagaatg 2700 ggcagacatt acgaatgcac acggtgtggt gggcccaggt attgttagcg gtttgaagca 2760 ggcggcagaa gaagtaacaa aggacctag aggccttttg atgttagcag attgtcatg 2820 caagggctcc ctactactg gagaatac taagggtact gttgacattg cgaagagcga 2880 ciaagatttt gttatcggct ttattgctca agagacatg ggtggaagg atgaaggtta 2940 cgattggttg attatgacac ccggtgtggg tttagatgac aagggacg cattgggtca 3000 acagtataga accgtggatg atgtggtctc tacaggatct gatatta ttgttggaag 3060 aggactattt gcaagggaa gggatgctaa ggtagagggt gaacgttaca gaaaagcagg 3120 ctgggaagca tatttgagaa gatgcggcca gcaaactaa aaactgtat tataagtaaa 3180 tgcatgtata ctaaactcac aaattagagc ttcaatttaa ttatatcagt tattacccgt 3240 aacttacacg cgcctcgtat cttatga tggaatatt tgggaattta ctctgtgttt 3300 atttatttt atgttttgta tttggatttt agaagtaa taagaaggt agaagagtta 3360 cggaatgaag aaaaaaaaat aaaaaggt ttaaaaaatt tcaaaaaa gcgtacttta 3420 catatatatt tattagacha gaaagcaga ttaatagat atacattcga tacgata 3480 gtaaaatgta aaatcacagg attttcgtgt gtggtctctct acacagacaa gatgaaaca 3540 ttcggcatta atacctgaga gcaggaagg caagataaaa ggtagtattt gttggcgatc 3600 cccctagagt cttttacatc ttcggaaac aaaaactatt tttctttaa ttcttttttt 3660 tactttctat ttttaattta tattaaaaatt taaatttaa tatttttat 3720 agcacgtgat gattcgtaa tcatgcacat ttccccgaaa agtgccacct gacgtctaag 3780 aaaccattat tatcatgaca ttacctata aaaataggcg tatcacgagg ccctttcgtc 3840 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 3900 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcaggcgggtg 3960 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 4020 accataaaat tgtaaacgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca 4080 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaatagc 4140 ccgagatagg gttgagtgtt gttccagttt ggaaagag tccactatta aagaacgtgg 4200 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat 4260 cacccaaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag 4320 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaagggga 4380 agaaaggaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa 4440 ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtactatggt tgctttgacg 4500 tatgcggtgt gaataccgc acagatgcgt aagagaaaa taccgcatca ggcgccattc 4560 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg 4620 ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc 4680 ccagtcacga cgttgtaaaa cgacggccag tgccaagctt gcatgcctgc a 4731 <210> 34 <211> 6738 <212> DNA <213> Artificial Sequence <400> 34 tatcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac 60 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac 120 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc 180 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg 240 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc 300 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt 360 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc 420 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa 480 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc 540 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg 600 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc 660 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc 720 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca 780 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact 840 acggctcac tagagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg 900 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt 960 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct 1020 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga 1080 gattatcaaa aggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa 1140 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac 1200 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga 1260 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc 1320 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca 1380 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta 1440 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg 1500 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc 1560 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg 1620 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt 1680 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt 1740 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata 1800 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc 1860 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac 1920 ccaactgatc ttcagcatct tttacttca ccagcgttc tggtgagca aaaacaggaa 1980 ggcaaaatgc cgcaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct 2040 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat 2100 ttgaatgtat ttagaaaat aaacaatag gggttccgcg ttcattcat cattttttt 2160 ttattcttt ttttgatttc gttttccttg aaattttt gattcggtaa tctccgaaca 2220 gaggaaa cgaaggaagg agcacagact tattgtagtgt 2280 tgagaaaca tgaaattgcc cagtattctt aacccaactg cacagaaaaaacctgcag 2340 gaaacgaga taaatcatgt cgaaagctac ataggaa cgtgctgcta ctcatcctag 2400 tcctgttgct gccaagctat ttaatcat gcacgaaag caacaact tgtgtgcttc 2460 attggatgtt cgtaccacca aggaattact ggagttagtt gaagcattag gtcccaaat 2520 ttgtttacta aaaacacatg tggatatctt gactgatttt tccatggagg gcacagttaa 2580 gccgctaaag gcattatccg ccaagtacaa ttttttatc ttcgaagaaca gaaaatttgc 2640 tgacattggt aatacagtca aattgcagta ctctgcgggt gtatacagaa tagcagaatg 2700 ggcagacatt acgaatgcac acggtgtggt gggcccaggt attgttagcg gtttgaagca 2760 ggcggcagaa aggaacctag aggccttttg atgttagcag aattgtcatg 2820 2880 caaagatttt gttatcggct ttattgctca aagagacatg ggtggagaag atgaaggtta 2940 cgattggttg attatgacac ccggtgtggg tttagatgac aagggagacg cattgggtca 3000 agattaga accgtggatg atgtggtctc tacaggatct gacattatta ttgttggaag 3060 aggactattt ccaaagggaa gggatgctaa ggtagagggt gaacgttaca gaaagcagg 3120 3180 tgcatgtata ctaaactcac aaattagagc ttcaatttaa ttatatcagt tattacccgt 3240 aacttacacg cgcctcgtat cttttaatga tggaataatt tgggaattta ctctgtgttt 3300 atttatttt atgttttgta tttggatttt agaagtaa taagaaggt agaagagtta 3360 cggaatgaag aaaaaaaaat aaaaaggt ttaaaaaatt tcaaaaaa gcgtacttta 3420 catatatatt tattagacha gaaagcaga ttaatagat atacattcga tacgata 3480 gtaaaatgta aaatcacagg attttcgtgt gtggtctctct acacagacaa gatgaaaca 3540 ttcggcatta atacctgaga gcaggaagg caagataaaa ggtagtattt gttggcgatc 3600 cccctagagt cttttacatc ttcggaaac aaaaactatt tttctttaa ttcttttttt 3660 tactttctat ttttaattta tattaaaaatt taaatttaa tatttttat 3720 agcacgtgat gattcgtaa tcatgcacat ttccccgaaa agtgccacct gacgtctaag 3780 aaaccattat tatcatgaca ttacctata aaaataggcg tatcacgagg ccctttcgtc 3840 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 3900 cagcttgtct gtaagcggat gccgggagca gataagccccg tcaggcggcg tcagcggggtg 3960 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 4020 accataaaat tgtaaacgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca 4080 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaatagc 4140 ccgagatagg gttgagtgtt gttccagttt ggaaagag tccactatta aagaacgtgg 4200 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat 4260 cacccaaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag 4320 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaagggga 4380 agaaaggaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa 4440 ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtactatggt tgctttgacg 4500 tatgcggtgt gaataccgc acagatgcgt aagagaaaa taccgcatca ggcgccattc 4560 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg 4620 ccagctggcg aaaggggat gtgctgcaag gcgattaagt tgggttaacgc cagggttttc 4680 ccagtcacga cgttgtaaaa cgacggccag tgccaagctt gcatgcctgc aactagtccg 4740 tcatgtccag gaagataggt cagttgattg gggatctagt gcaaagga tcgttgaagg 4800 aagaggtcct atggaatcta tcggaccgcg aattttggga gctcctgaaa agtaaagttg 4860 actctaaag attggagacg attaaaaca tcgaagcagg gccgcatgcg gaagattatc 4920 atcgtctacc tcgtggtacc aagattcgca caatcgatcc tgatctgctc ctcccgggtg 4980 ctgggcaacc ctccccactg tcttttgtga aacctgaatg ggctaaggag agaagaact 5040 tcattcaggc tcgccaggcc ttgtttatag actagcatct tttactgtct acatcaacgg 5100 cggcgtgagc tgggaacaag atagtattca gccacaattc gagaacaatg tatcacttta 5160 agcttaattt ataaagac catattcact tctcacaaaa aatgcctctt ttcaccagta 5220 gatggtagcg ttctgatatg gcggggttga ctgcctcagg cctgcaaccc cgccgcaaaa 5280 aaagacccgc ttatcgatta tcgtccccaa ctttttatga ctcgacatca tctccagaca 5340 ctttcctctt catactccca attctacaga atcccatcat gtctgaagct ctgacaaaca 5400 ccgaccttca gggtgctctc cctctcatcg ctcgaggcaa ggttcgcgac ctgtacgatg 5460 tcgacgagaa gaccctcctc ttcgtcgcaa cggaccgcat ctccgcgtac gatgtgatca 5520 tggagaatgt accactccct cagttctcaa taacagtacc agtaccacta acagcaatga 5580 aagggaatcc ccgagaaagg catcctccta actctatgca cgaaaacctg gttcaagatc 5640 ctctccgaca aaatcccctc tctccgcacc cacttcctca ccctcgacct cccgcctcag 5700 atccccgagt cgttgcgtcc cgtcctccag aacagaagca tgcaggtgcg caagctgaag 5760 atcctcccca ttgaggccat cgtccgcggt tacatcaccg gttccgcctg gaacgagtac 5820 aagaaatccg gcaccgtgca cgggatcaag gtcgccgagg gtctgaggga gagcgaggcc 5880 ttccctgatg gtcctattta cactcccagt accaaggcgg agcagggcga gcatgatgag 5940 aacattcatc cggatcaggg tatgttctat ctatatctcc gtttcttccc gggttccgat 6000 ttcggaggca gtggttgatt tgccttgttt aattctcttt tctcttctct ctttttttta 6060 aaaaaatatg tggctgtaga ttattggcta attgtttggt gctgtagctg tcgctattgt 6120 tggtgaacgt tatgcctcta agatcgcctc tcttgcggtt cagctttaca aggtcgctca 6180 cgagtatgcc cttactcgtg gtgtgatcat tgccgacacc aagttcgagt tcggtttgga 6240 tcctgagact gacgaggttg tgctggccga cgaggtcctc acccccgatt catcgcgttt 6300 ctggcctaag gactcgtacg agatcggtcg tggacagcag agcttcgata agcagttctt 6360 gcgtgactgg ttgacgagcg agggactgaa gggcaagcct ggtgtgcgga tgaccgatga 6420 gattgcgcag aagaccagcg ctaagtaccg ggaggcatat gagcggatca ctggtgagac 6480 taaggttcct gcggtctagt cagggaggtg gtgtatgtca aaatgaaatt gtcgtatata 6540 taacgctcaa ttgtccaatg tctataaatc cgtcttaccc aagtcattgg tcaagatgaa 6600 aactggtata gagacctaaa aatataaatt actatacaaa ttgaacctgg tatccattta 6660 ctgatcccac tcggaacgca acgccttcaa tcccttcttc gctttcggcg cctcctgtgg 6720 actcgtatgc tgttgcgc 6738 <210> 35 <211> 44 <212> DNA <213> Artificial Sequence <400> 35 tcacaccaca acaccgccac catgtctcct gacctgacat acac 44 <210> 36 <211> 47 <212> DNA <213> Artificial Sequence <400> 36 cttcacgagc tactacagat cctatttttc cagcttggct gccttac <210> 37 <211> 42 <212> DNA <213> Artificial Sequence <400> 37 atgtcaggtc aggagacatg gtggcggtgt tgtggtgtga ag <210> 38 <211> 50 <212> DNA <213> Artificial Sequence <400> 38 ggcagccaag ctggaaaaat aggatctgta gtagctcgtg aagggtggag <210> 39 <211> 44 <212> DNA <213> Artificial Sequence <400> 39 44. tcacaccaca acaccgccac catgcgatac caatattctg agag <210> 40 <211> 45 <212> DNA <213> Artificial Sequence <400> 40 cttcacgagc tactacagat cctatagctt actgtggtag actgg <210> 41 <211> 42 <212> DNA <213> Artificial Sequence <400> 41 cagaatattg gtatcgcatg gtggcggtgt tgtggtgtga ag <210> 42 <211> 46 <212> DNA <213> Artificial Sequence <400> 42 gtctaccaca gtaagctata ggatctgtag tagctcgtga agggtg <210> 43 <211> 41 <212> DNA <213> Artificial Sequence <400> 43 cackle caccgccacc atgcctacca gaaagagaga c <210> 44 <211> 42 <212> DNA <213> Artificial Sequence <400> 44 cttcacgagc tactacagat cctactgaat cggaaggcgc ag <210> 45 <211> 42 <212> DNA <213> Artificial Sequence <400> 45 ctgcgccttc cgattcagta ggatctgtag tagctcgtga ag <210> 46 <211> 41 <212> DNA <213> Artificial Sequence <400> 46 gtctctcttt ctggtaggca tggtggcggt gttgtggtgt g 41 <210> 47 <211> 40 <212> DNA <213> Artificial Sequence <400> 47 caccacaaca ccgccaccat gcctccatct atcttgaacg 40 <210> 48 <211> 40 <212> DNA <213> Artificial Sequence <400> 48 cacgagctac tacagatctc aagcggttgt gggagcgttg 40 <210> 49 <211> 43 <212> DNA <213> Artificial Sequence <400> 49 caacgctccc acaaccgctt gagatctgta gtagctcgtg aag 43 <210> 50 <211> 41 <212> DNA <213> Artificial Sequence <400> 50 gttcaagata gatggaggca tggtggcggt gttgtggtgt g 41 <210> 51 <211> 46 <212> DNA <213> Artificial Sequence <400> 51 gtagaccggt tgaccattaa ttgtattggt attaccagaa cgtcac 46 <210> 52 <211> 48 <212> DNA <213> Artificial Sequence <400> 52 cttcacacca caacaccgcc accatgtttg acgtgctctt tgaccatg 48 <210> 53 <211> 45 <212> DNA <213> Artificial Sequence <400> 53 ttctggtaat accaatacaa ttaatggtca accggtctac agaag 45 <210> 54 <211> 48 <212> DNA <213> Artificial Sequence <400> 54 aagagcacgt caaacatggt ggcggtgttg tggtgtgaag ggtgattg 48 <210> 55 <211> 46 <212> DNA <213> Artificial Sequence <400> 55 atggactctt gccaatgtga ttgtattggt attaccagaa cgtcac 46 <210> 56 <211> 47 <212> DNA <213> Artificial Sequence <400> 56 cttcacacca caacaccgcc accatgaaga tcgtcgtcgt tggcgcc 47 <210> 57 <211> 44 <212> DNA <213> Artificial Sequence <400> 57 ttctggtaat accaatacaa tcacattggc aagagtccat ctgc 44 <210> 58 <211> 48 <212> DNA <213> Artificial Sequence <400> 58 acgacgacga tcttcatggt ggcggtgttg tggtgtgaag ggtgattg 48 <210> 59 <211> 49 <212> DNA <213> Artificial Sequence <400> 59 ggtactgtga cgaccatggt ggccagcccc tcacggaacc ccgaccaac 49 <210> 60 <211> 47 <212> DNA <213> Artificial Sequence <400> 60 ggttccgtga ggggctggcc accatggtcg tcacagtacc cactgcc 47 <210> 61 <211> 45 <212> DNA <213> Artificial Sequence <400> 61 cctatgcgaa cccgtctaga ctactgcgac gagccaccag agatg 45 <210> 62 <211> 45 <212> DNA <213> Artificial Sequence <400> 62 ctggtggctc gtcgcagtag tctagacggg ttcgcatagg tttgg 45 <210> 63 <211> 46 <212> DNA <213> Artificial Sequence <400> 63 cccgaaagct cagcgcttga ttgtattggt attaccagaa cgtcac 46 <210> 64 <211> 47 <212> DNA <213> Artificial Sequence <400> 64 cttcacacca caacaccgcc accatgaata atcaactttt actggcc 47 <210> 65 <211> 44 <212> DNA <213> Artificial Sequence <400> 65 ttctggtaat accaatacaa tcaagcgctg agctttcggg gctc 44 <210> 66 <211> 48 <212> DNA <213> Artificial Sequence <400> 66 aaaagttgat tattcatggt ggcggtgttg tggtgtgaag ggtgattg 48 <210> 67 <211> 46 <212> DNA <213> Artificial Sequence <400> 67 ttactccggc tacgtactag ttgtattggt attaccagaa cgtcac 46 <210> 68 <211> 47 <212> DNA <213> Artificial Sequence <400> 68 cttcacacca caacaccgcc accatgtctc aatcctacaa atcaagc 47 <210> 69 <211> 45 <212> DNA <213> Artificial Sequence <400> 69 ttctggtaat accaatacaa ctagtacgta gccggagtaa cgctg 45 <210> 70 <211> 48 <212> DNA <213> Artificial Sequence <400> 70 ttgtaggatt gagacatggt ggcggtgttg tggtgtgaag ggtgattg 48 <210> 71 <211> 49 <212> DNA <213> Artificial Sequence <400> 71 49. tcggcaagag acaccatggt ggccagcccc tcacggaacc ccgaccaac <210> 72 <211> 48 <212> DNA <213> Artificial Sequence <400> 72 ggttccgtga ggggctggcc accatggtgt ctcttgccga cttgcttc <210> 73 <211> 45 <212> DNA <213> Artificial Sequence <400> 73 cctatgcgaa cccgtctaga ttaaggggcc tcgacgtag agctg <210> 74 <211> 45 <212> DNA <213> Artificial Sequence <400> 74 tctacgtcga ggccccttaa tctagacggg ttcgcatagg tttgg <210> 75 <211> 46 <212> DNA <213> Artificial Sequence <400> 75 gttttcgcag gcgagaatag ttgtattggt attack cgtcac <210> 76 <211> 47 <212> DNA <213> Artificial Sequence <400> 76 47. cttcacacca caacaccgcc accatggagt acggcatcct ccttctg <210> 77 <211> 44 <212> DNA <213> Artificial Sequence <400> 77 ttctggtaat accataca ctattctcgc ctgcgaaac gag <210> 78 <211> 48 <212> DNA <213> Artificial Sequence <400> 78 aggatgccgt actccatggt ggcggtgttg tggtgtgaag ggtgattg <210> 79 <211> 46 <212> DNA <213> Artificial Sequence <400> 79 agtctacagc agcccagtaa ttgtattggt attack cgtcac <210> 80 <211> 47 <212> DNA <213> Artificial Sequence <400> 80 47. cttcacacca cacaccgcc accatgtatc ccatcttaac cgcggcc <210> 81 <211> 45 <212> DNA <213> Artificial Sequence <400> 81 ttctggtaat accaatacaa ttactgggct gctgtagact ggatg 45 <210> 82 <211> 48 <212> DNA <213> Artificial Sequence <400> 82 gttaagatgg gatacatggt ggcggtgttg tggtgtgaag ggtgattg 48 <210> 83 <211> 49 <212> DNA <213> Artificial Sequence <400> 83 actatttggt caatcatggt ggccagcccc tcacggaacc ccgaccaac 49 <210> 84 <211> 47 <212> DNA <213> Artificial Sequence <400> 84 ggttccgtga ggggctggcc accatgattg accaaatagt gtcccac 47 <210> 85 <211> 45 <212> DNA <213> Artificial Sequence <400> 85 cctatgcgaa cccgtctaga tcatggagac cttctctcct tgaac 45 <210> 86 <211> 44 <212> DNA <213> Artificial Sequence <400> 86 aggagagag gtctccatga tctagacggg ttcgcatagg tttg <210> 87 <211> 53 <212> DNA <213> Artificial Sequence <400> 87 aaggatgtct gagagctcgg tacccgggga tctgtagtag ctcgtgaagg gtg <210> 88 <211> 47 <212> DNA <213> Artificial Sequence <400> 88 cagcaagctc cgaattcgcc accatggccc ctcacgctga gattgac <210> 89 <211> 52 <212> DNA <213> Artificial Sequence <400> 89 ctactacaga tccccgggta ccgagctctc agacatcctt ctcgtcccaa ag <210> 90 <211> 52 <212> DNA <213> Artificial Sequence <400> 90 tcagcgtgag gggccatggt ggcgaattcg gagcttgctg tggggtttat tg <210> 91 <211> 49 <212> DNA <213> Artificial Sequence <400> 91 gcgtccgtaa cagccatggt ggccagcccc tcacggaacc ccgaccaac 49 <210> 92 <211> 47 <212> DNA <213> Artificial Sequence <400> 92 ggttccgtga ggggctggcc accatggctg ttacggacgc gacagac 47 <210> 93 <211> 44 <212> DNA <213> Artificial Sequence <400> 93 cctatgcgaa cccgtctaga tcagtcttgc gtgatgtgac ctag 44 <210> 94 <211> 45 <212> DNA <213> Artificial Sequence <400> 94 gtcacatcac gcaagactga tctagacggg ttcgcatagg tttgg 45 <210> 95 <211> 53 <212> DNA <213> Artificial Sequence <400> 95 aagcgtcgct aagagctcgg tacccgggga tctgtagtag ctcgtgaagg gtg 53 <210> 96 <211> 47 <212> DNA <213> Artificial Sequence <400> 96 cagcaagctc cgaattcgcc accatggccg tcgtcactgt gtcagac 47 <210> 97 <211> 52 <212> DNA <213> Artificial Sequence <400> 97 ctactacaga tccccgggta ccgagctctt agcgacgctt aaagagcaga ag 52 <210> 98 <211> 52 <212> DNA <213> Artificial Sequence <400> 98 acagtgacga cggccatggt ggcgaattcg gagcttgctg tggggtttat tg 52 <210> 99 <211> 45 <212> DNA <213> Artificial Sequence <400> 99 cccggttgga gttcggatcc tacagcatgg tctggattcc aatcc 45 <210> 100 <211> 45 <212> DNA <213> Artificial Sequence <400> 100 ggaatccaga ccatgctgta ggatccgaac tccaaccggg gggag 45 <210> 101 <211> 49 <212> DNA <213> Artificial Sequence <400> 101 aagttgaggg atgtcatggt ggccagcccc tcacggaacc ccgaccaac 49 <210> 102 <211> 48 <212> DNA <213> Artificial Sequence <400> 102 ggttccgtga ggggctggcc accatgacat ccctcaactt cactctag 48 <210> 103 <211> 44 <212> DNA <213> Artificial Sequence <400> 103 cctatgcgaa cccgtctaga ctatacggag aaggggcagc ctac 44 <210> 104 <211> 45 <212> DNA <213> Artificial Sequence <400> 104 gctgcccctt ctccgtatag tctagacggg ttcgcatagg tttgg 45 <210> 105 <211> 44 <212> DNA <213> Artificial Sequence <400> 105 atcagtctag aggatcccca ctctagtcta cagtggccgc cttg 44 <210> 106 <211> 45 <212> DNA <213> Artificial Sequence <400> 106 gcggccactg tagactagag tggggatcct ctagactgat gatgc 45 <210> 107 <211> 44 <212> DNA <213> Artificial Sequence <400> 107 tgtatatgtg taggaagatc ctctagtcta cagtggccgc cttg 44 <210> 108 <211> 44 <212> DNA <213> Artificial Sequence <400> 108 gcggccactg tagactagag gatcttccta cacatataca ggac 44 <210> 109 <211> 46 <212> DNA <213> Artificial Sequence <400> 109 tctacatcat caagcactga ttgtattggt attaccagaa cgtcac 46 <210> 110 <211> 48 <212> DNA <213> Artificial Sequence <400> 110 cagcaagctc cgaattcgcc accatggata ccataggatt cattcgtg 48 <210> 111 <211> 45 <212> DNA <213> Artificial Sequence <400> 111 ttctggtaat accaatacaa tcagtgcttg atgatgtaga cgtac 45 <210> 112 <211> 54 <212> DNA <213> Artificial Sequence <400> 112 atcagtctag aggatcccca tcatggtgtt ttgatcattt taaattttta tatg 54 <210> 113 <211> 52 <212> DNA <213> Artificial Sequence <400> 113 aatcctatgg tatccatggt ggcgaattcg gagcttgctg tggggtttat tg 52 <210> 114 <211> 45 <212> DNA <213> Artificial Sequence <400> 114 aaatgatcaa aacaccatga tggggatcct ctagactgat gatgc 45 <210> 115 <211> 47 <212> DNA <213> Artificial Sequence <400> 115 tccgaccgga acttcactca tatcgtaatc atggtcatag ctgtttc 47 <210> 116 <211> 49 <212> DNA <213> Artificial Sequence <400> 116 ctgtatatgt gtaggaagat catcttggat ataaaaatcc aaaatatgg 49 <210> 117 <211> 47 <212> DNA <213> Artificial Sequence <400> 117 tggattttta tatccaagat gatcttccta cacatataca ggacatg 47 <210> 118 <211> 46 <212> DNA <213> Artificial Sequence <400> 118 ctatgaccat gattacgata tgagtgaagt tccggtcgga gcaatc 46 <210> 119 <211> 47 <212> DNA <213> Artificial Sequence <400> 119 tgtatatgtg taggaagatc gcgcaacagc atacgagtcc acaggag 47 <210> 120 <211> 45 <212> DNA <213> Artificial Sequence <400> 120 tggactcgta tgctgttgcg cgatcttcct acacatatac aggac 45 <210> 121 <211> 48 <212> DNA <213> Artificial Sequence <400> 121 gcggccactg tatcgtaatc atggtcatag ctgtttcc <210> 122 <211> 48 <212> DNA <213> Artificial Sequence <400> 122 cccggttgga gttcggatcc tgagtgaagt tccggtcgga gcaatcag 48 <210> 123 <211> 46 <212> DNA <213> Artificial Sequence <400> 123 ctccgaccgg aacttcactc aggatccgga ctccaaccgg ggggag <210> 124 <211> 44 <212> DNA <213> Artificial Sequence <400> 124 ctatgaccat gattacgata ctctagtcta cagtggccgc cttg <210> 125 <211> 44 <212> DNA <213> Artificial Sequence <400> 125 tacgggtcac gatcggatga ttgtattggt attack cgtc <210> 126 <211> 49 <212> DNA <213> Artificial Sequence <400> 126 cttcacacca caacaccgcc accatgacca tttcactcag ctacctatc 49 <210> 127 <211> 44 <212> DNA <213> Artificial Sequence <400> 127 ttctggtaat accaatacaa tcatccgatc gtgacccgta tgcc 44 <210> 128 <211> 48 <212> DNA <213> Artificial Sequence <400> 128 ctgagtgaaa tggtcatggt ggcggtgttg tggtgtgaag ggtgattg 48 <210> 129 <211> 44 <212> DNA <213> Artificial Sequence <400> 129 cacttccatg gtacccgtaa ttgtattggt attaccagaa cgtc 44 <210> 130 <211> 47 <212> DNA <213> Artificial Sequence <400> 130 cttcacacca caacaccgcc accatggctc ctccaacagt agttctc 47 <210> 131 <211> 44 <212> DNA <213> Artificial Sequence <400> 131 ttctggtaat accaatacaa ttacgggtac catggaagtg gagc 44 <210> 132 <211> 48 <212> DNA <213> Artificial Sequence <400> 132 actgttggag gagccatggt ggcggtgttg tggtgtgaag ggtgattg 48 <210> 133 <211> 49 <212> DNA <213> Artificial Sequence <400> 133 cttcacacca caacaccgcc accatgacca tttcactcag ctacctatc 49 <210> 134 <211> 49 <212> DNA <213> Artificial Sequence <400> 134 gcagcaaaag agcgcatggt ggccagcccc tcacggaacc ccgaccaac 49 <210> 135 <211> 48 <212> DNA <213> Artificial Sequence <400> 135 ggttccgtga ggggctggcc accatgcgct cttttgctgc agcaacac 48 <210> 136 <211> 45 <212> DNA <213> Artificial Sequence <400> 136 cctatgcgaa cccgtctaga ttactcgccc tgcagcggct gcccg 45 <210> 137 <211> 45 <212> DNA <213> Artificial Sequence <400> 137 agccgctgca gggcgagtaa tctagacggg ttcgcatagg tttgg 45 <210> 138 <211> 48 <212> DNA <213> Artificial Sequence <400> 138 cccggttgga gttcggatcc tgagtgaagt tccggtcgga gcaatcag 48 <210> 139 <211> 47 <212> DNA <213> Artificial Sequence <400> 139 ggttccgtga ggggctggcc accatgattg accaaatagt gtcccac 47 <210> 140 <211> 45 <212> DNA <213> Artificial Sequence <400> 140 ctattatagg aaaggcgcgc cctctagtct acagtggccg ccttg 45 <210> 141 <211> 54 <212> DNA <213> Artificial Sequence <400> 141 gcggccactg tagactagag ggcgcgcctt tcctataata gactagcgtg cttg 54

Claims

1. A terpenoid biosynthesis gene cluster, characterized in that: The terpenoid compound is a sesquiterpene; the biosynthetic gene cluster of the terpenoid compound is; Gene cluster BGC1: includes Aat08891 gene, Aat08894 gene, Aat08897 gene and Aat08898 gene, which encode Monooxygenase, Reductase, TS and P450 enzymes, respectively. Their nucleotide sequences are shown in SEQ ID NO.5 to SEQ ID NO.8, respectively.

2. The terpenoid biosynthesis gene cluster according to claim 1, characterized in that: The nucleotide sequences of the gene cluster BGC1 are shown in SEQ ID NO.

1.

3. A nucleic acid molecule, characterized in that: It encodes the enzyme of the biosynthetic gene cluster as claimed in claim 1.

4. An expression cassette for a terpenoid biosynthesis gene cluster, characterized in that: The expression cassette contains the terpenoid biosynthesis gene cluster according to claim 1 or the nucleic acid molecule according to claim 3; it also includes a promoter and a terminator; the promoter includes one of PhlyA, PamyB and PglaA, and the terminator includes one of TagdA, TamyB and TniaD.

5. An expression vector for a terpenoid biosynthesis gene cluster, characterized in that: The expression vector contains the expression cassette according to claim 4.

6. A method for preparing an expression vector for a terpenoid biosynthesis gene cluster, characterized in that: The method comprises: The linearized vector containing the promoter and terminator and the terpenoid biosynthesis gene cluster according to claim 1 are amplified separately, mixed and then transferred into Saccharomyces cerevisiae competent cells to obtain the product.

7. A recombinant microbial strain of a terpenoid biosynthetic gene cluster, characterized in that: The recombinant microbial strain contains the expression vector according to claim 5.

8. The recombinant microbial strain of a terpenoid biosynthetic gene cluster according to claim 7, characterized in that: The microbial strain comprises one of Aspergillus oryzae, Escherichia coli, Saccharomyces cerevisiae, Pichia pastoris, Bacillus, Aspergillus nidulans, Aspergillus niger, Neurospora crassa, Alternaria alternata or Fusarium.

9. A method for biosynthesis of terpenoid compounds, characterized in that: The method comprises: Constructing a microbial mutant strain that heterologously synthesizes the biosynthetic gene cluster of the terpenoid compound according to claim 1; fermenting the microbial mutant to obtain a fermentation culture; The fermentation culture is extracted, separated and the product is purified to obtain terpenoid compounds.

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