Method for determining karst plants with rapid seed germination

Abstract

The present application provides a method for determining a seed-rapid germination type karst plant, and relates to the technical field of vegetation restoration. The method for determining a seed-rapid germination type karst plant comprises: analyzing the configuration category of the embryo and the morphological characteristics of the endosperm of the seeds of the karst plant to be determined. If the configuration category of the embryo of the seed meets the target configuration subtype I and the endosperm is missing or degenerated, the karst plant to be determined is determined to be a rapid germination type; otherwise, the karst plant to be determined is determined to be a non-rapid germination type; the target configuration subtype I includes any one of a spoon type, a straight type, a lateral base type, a stacked type, a peripheral type or a filled type. The method for determining a seed-rapid germination type karst plant provided in the present application adopts a simple and easy biological analysis method, and can accurately and universally determine whether it is a rapid germination type plant by analyzing and determining the configuration category of the plant seed embryo and the morphological characteristics of the endosperm.

Description

Technical Field

[0001] The present application relates to the technical field of vegetation restoration, and in particular to a method for determining fast-germinating karst plants. Background Art

[0002] Karst landform is an important part of terrestrial ecosystem. Under the influence of monsoon climate and human activities, it is very easy to cause rocky desertification landscapes such as soil erosion, exposed bedrock accumulation, and vegetation degradation. Under ecological engineering measures such as returning farmland to forests and closing mountains to cultivate forests, the xeric bare land undergoes slow secondary succession and gradually transitions to the herbaceous plant community, grass shrubs, and tree shrubs. However, relying on natural succession for vegetation restoration will result in a long cycle, unstable restored vegetation structure, and increased probability of secondary disasters. Seeds are carriers of genetic genes and the most critical components for plant reproduction. How to use plant seeds to accelerate the process of vegetation restoration provides a new idea for rocky desertification control. However, in the long-term adaptive evolution of karst plants, some plant seeds form dormancy, resulting in the inability of seeds to germinate into seedlings in a short period of time, hindering vegetation renewal and having a negative impact on the rapid recovery of regional vegetation. Therefore, how to quickly and simply screen out fast-germinating karst plants is of great significance to promoting vegetation renewal and accelerating the secondary succession of degraded karst vegetation. Summary of the invention

[0003] The purpose of this application is to provide a method for determining fast-germinating karst plants to solve the above problems.

[0004] To achieve the above objectives, this application adopts the following technical solutions:

[0005] A method for determining a seed rapid germination type karst plant, comprising:

[0006] Analyze the embryo configuration category and endosperm morphological characteristics of the seeds of the karst plant to be identified. If the embryo configuration category of the seeds meets the target configuration subtype I and the endosperm is missing or degenerated, the karst plant to be identified is determined to be a rapid germination type; otherwise, the karst plant to be identified is determined to be a non-rapid germination type;

[0007] The target configuration subtype I includes any one of spoon type, straight type, side base type, stacked type, peripheral type or filled type.

[0008] Preferably, if the configuration category of the embryo of the seed is subtype II, the karst plant to be determined is determined to be a non-rapid germination type;

[0009] The subtype II includes any one of the head type, cell type, ball type or spindle type.

[0010] Preferably, if the endosperm of the seed is thick or lignified, the karst plant to be identified is determined to be a non-rapid germination type.

[0011] Preferably, the configuration category of the embryo and the morphological characteristics of the endosperm of the karst plant seeds to be identified are analyzed in sequence.

[0012] Preferably, before performing the analysis, the method further includes: pre-processing the seeds of the karst plants to be identified.

[0013] Preferably, the pretreatment comprises: embedding the seeds in water-containing vermiculite, and then peeling off the seed coat, endosperm and embryo in sequence.

[0014] Preferably, the water content of the vermiculite is 15-20wt%, and the particle size of the vermiculite is 1-3mm.

[0015] Preferably, the embedding temperature is 20-25°C and the time is 12-24h.

[0016] Preferably, the method for collecting seeds of the karst plant to be determined comprises: collecting the fruits of the plant during the fruiting period of the plant, then removing the pulp of the fruit, removing impurities and drying it, and then selecting plump seeds.

[0017] Preferably, when performing the collection, the number of plants collected is not less than 10.

[0018] Compared with the prior art, the beneficial effects of this application include:

[0019] The method for determining fast-germinating karst plants provided in the present application adopts a simple and easy biological analysis method. By analyzing and determining the configuration category of the plant seed embryo and the morphological characteristics of the endosperm, it can accurately and universally determine whether it is a fast-germinating plant. The determination method is applied to screening fast-germinating plants from a variety of plant seeds for restoration of degraded karst vegetation. The screened plants are highly representative, their seeds are easy to germinate and have a fast germination speed, and they are highly adaptable to environments with relatively scarce water resources. This can greatly improve the success rate of degraded karst ecological restoration based on plant seeds. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] In order to more clearly illustrate the technical solutions of the embodiments of the present application, the drawings required for use in the embodiments will be briefly introduced below. It should be understood that the following drawings only show certain embodiments of the present application and therefore should not be regarded as limiting the scope of the present application.

[0021] Figure 1 The schematic diagram of the target configuration subtype I of the embryo of the seed;

[0022] Figure 2 The schematic diagram of the target configuration subtype II for the embryo of the seed;

[0023] Figure 3These are some actual photos of the embryo structure and endosperm morphological characteristics of karst plant seeds. DETAILED DESCRIPTION

[0024] As used herein:

[0025] "Prepared from" is synonymous with "comprising." As used herein, the terms "comprising," "including," "having," "containing," or any other variation thereof, are intended to cover a non-exclusive inclusion. For example, a composition, process, method, article, or apparatus that comprises the listed elements is not necessarily limited to only those elements but may include other elements not expressly listed or inherent to such composition, process, method, article, or apparatus.

[0026] The conjunction "consisting of excludes any unspecified element, step, or component. If used in a claim, this phrase renders the claim closed-ended so that it does not include materials other than those described, except for conventional impurities associated therewith. When the phrase "consisting of" appears in a clause of the body of a claim rather than immediately following the subject matter, it limits only the elements described in that clause; other elements are not excluded from the claim as a whole.

[0027] When an amount, concentration, or other value or parameter is expressed as a range, a preferred range, or a range defined by a series of upper preferred values ​​and lower preferred values, this should be understood as specifically disclosing all ranges formed by any pairing of any range upper limit or preferred value with any range lower limit or preferred value, regardless of whether the range is disclosed separately. For example, when a range of "1 to 5" is disclosed, the described range should be interpreted as including ranges "1 to 4", "1 to 3", "1 to 2", "1 to 2 and 4 to 5", "1 to 3 and 5", etc. When a numerical range is described in this article, unless otherwise stated, the range is intended to include its end values ​​and all integers and fractions within the range.

[0028] In these examples, parts and percentages are by mass unless otherwise indicated.

[0029] "Parts by mass" refers to the basic unit of measurement for expressing the mass ratio of multiple components. 1 part can represent any unit mass, such as 1g or 2.689g. If we say that the mass of component A is a parts and the mass of component B is b parts, it means the ratio of the mass of component A to the mass of component B is a:b. Alternatively, it means that the mass of component A is aK and the mass of component B is bK (K is an arbitrary number, indicating a multiple factor). It should not be misunderstood that, unlike the mass parts, the sum of the mass of all components is not limited to 100 parts.

[0030] "And / or" is used to indicate that one or both of the stated situations may occur, for example, A and / or B includes (A and B) and (A or B).

[0031] First, the method provided by this application is explained as follows:

[0032] A method for determining a seed rapid germination type karst plant, comprising:

[0033] Analyze the embryo configuration category and endosperm morphological characteristics of the seeds of the karst plant to be determined. If the embryo configuration category of the seeds meets the target configuration subtype I and the endosperm is missing or degenerated (thin), the karst plant to be determined is determined to be a fast germination type; otherwise, the karst plant to be determined is determined to be a non-fast germination type;

[0034] The target configuration subtype I includes any one of spoon type, straight type, side base type, stacked type, peripheral type or filled type.

[0035] If any one of the embryo configuration category and endosperm morphological characteristics does not meet the requirements, the corresponding plant can be judged as a non-rapid germination type.

[0036] like Figure 1 As shown, for the specific target configuration subtype I:

[0037] (1) Spoon-shaped: The embryonic root end is upright and protrudes, and the embryo as a whole is shaped like a short spoon. Figure 1 a);

[0038] (2) Straight type: The embryo is upright, and the length of the embryo is much greater than its width. Figure 1 b);

[0039] (3) Lateral type: The embryo is located on one side of the seed base and extends along the edge of the seed coat toward the top. Figure 1 c);

[0040] (4) Peripheral type: The embryo is long and curved, and the embryo extends along the seed coat ( Figure 1 middle d);

[0041] (5) Overlapping: The embryo is short-spoon-shaped as a whole, but the embryo axis is folded ( Figure 1 e);

[0042] (6) Filling type: The radicle end is short and upright, and the embryo fills the inside of the seed without a cavity. Figure 1 (f)

[0043] In an optional embodiment, if the configuration category of the embryo of the seed is subtype II, the karst plant to be determined is determined to be a non-rapid germination type;

[0044] The subtype II includes any one of the head type, cell type, ball type or spindle type.

[0045] like Figure 2 As shown, for the specific target configuration subtype II:

[0046] (1) Head shape: The embryo is enlarged and head-shaped, located at the base of the seed ( Figure 2 a);

[0047] (2) Cell type: The embryo morphology is difficult to distinguish and may be a single cell or a cell cluster. Figure 2 b);

[0048] (3) Spherical: The embryo is spherical and located at the base of the seed ( Figure 2 c);

[0049] (4) Fusiform: The embryo is fusiform and located at the base of the seed ( Figure 2 (d)

[0050] In an optional embodiment, if the endosperm of the seed is thick or lignified, the karst plant to be identified is determined to be a non-rapid germination type.

[0051] In an optional embodiment, the configuration category of the embryo and the morphological characteristics of the endosperm of the karst plant seeds to be identified are analyzed in sequence.

[0052] From the perspective of saving operation time and cost, if the configuration category of a seed embryo does not meet any of the subtypes I (spoon-shaped, straight, lateral-based, stacked, peripheral or filled), the seed needs to undergo embryo configuration transformation before initiating germination. For this process to occur, the embryo needs to obtain more energy supply and longer configuration transformation time, which greatly delays the initiation of seed germination. If a seed endosperm is hypertrophic or lignified, the mechanical constraint on the embryo is too strong, resulting in the obstruction of normal embryo elongation and structural differentiation (radicle, plumule, hypocotyl formation), which seriously inhibits the germination process. Therefore, when judging whether a plant is a fast-germinating plant, the configuration category of the seed embryo can be used as the first prerequisite, and the morphological characteristics of the seed endosperm can be used as the second prerequisite.

[0053] But it should be noted that the judgment order of the two conditions can be swapped.

[0054] In an optional embodiment, before performing the analysis, the method further includes: pre-processing the seeds of the karst plants to be identified.

[0055] In an optional embodiment, the pretreatment includes: embedding the seeds in water-containing vermiculite, and then peeling off the seed coat, endosperm and embryo in sequence.

[0056] In an optional embodiment, the water content of the vermiculite is 15-20 wt %, and the particle size of the vermiculite is 1-3 mm.

[0057] Optionally, the water content of the vermiculite may be 15wt%, 16wt%, 17wt%, 18wt%, 19wt%, 20wt% or any value between 15-20wt%, and the particle size of the vermiculite may be 1mm, 2mm, 3mm or any value between 1-3mm.

[0058] In an optional embodiment, the embedding temperature is 20-25°C and the time is 12-24h.

[0059] Optionally, the embedding temperature can be 20°C, 21°C, 22°C, 23°C, 24°C, 25°C or any value between 20-25°C, and the embedding time can be 12h, 14h, 16h, 18h, 20h, 22h, 24h or any value between 12-24h.

[0060] The temperature and time of embedding can be adjusted appropriately according to the plant species.

[0061] In an optional embodiment, the method for harvesting seeds of the karst plant to be determined includes: collecting the fruits of the plants during the fruiting period of the plants, then removing the pulp of the fruits, removing impurities and drying them, and then selecting plump seeds.

[0062] In an optional embodiment, when performing the collection, the number of plants collected is not less than 10.

[0063] The embodiments of the present application will be described in detail below in conjunction with specific examples, but it will be appreciated by those skilled in the art that the following examples are only used to illustrate the present application and should not be considered as limiting the scope of the present application. If specific conditions are not specified in the examples, they are carried out according to normal conditions or the conditions recommended by the manufacturer. If the manufacturer is not specified for the reagents or instruments used, they are all conventional products that can be purchased commercially.

[0064] First, the sources of research materials for this application and their basic situation are explained as follows:

[0065] The study area is located in Fenglin Village and Heping Village, Pingshang Town, northeast of Puding County, Anshun City, Guizhou Province. The area is a typical degraded karst landform with buried and exposed limestone, and the soil is typical yellow-brown lime soil. The region has distinct dry seasons (October to April of the following year) and rainy seasons (May to September) throughout the year, with typical subtropical rain and heat meteorological characteristics. Under a series of engineering measures such as returning farmland to forests and closing mountains for afforestation, the regional vegetation has undergone secondary succession, transitioning from bare land to other vegetation types. At present, grass, shrub grass and sparse shrubs are the typical main vegetation types in the area. The covering vegetation mainly includes five life forms: herbs, shrubs, vines, shrubs / small trees, and trees. The fruiting period of most plants is from August to November.

[0066] The main herbaceous plants in the study area are Cruciferae, Dipsacus asper, Imperata chinensis, Coleus chinensis and Fagopyrum serrata; the main shrubs are Buddleja, White Pearl of Yunnan, Viburnum chinense, Chaotian Can, Pyracantha, Rosa microcarpa, Rosa rubrum, Bulbous sorghum and Spiraea; the main shrubs / small trees are Whitethorn, Rhamnus lancifolius, Zanthoxylum bungeana, Rhizoma yunnanensis, Sorbus australis and Rhododendron truncatum; the main vines are Celastrus orbiculatus and Polygonum aviculare; the main trees are Alnus truncatula, Carpinus yunnanensis, Catalpa ovata, Cypress yunnanensis and Toona sinensis.

[0067] Example 1

[0068] This embodiment provides a method for determining seed-rapid germination karst plants (the research objects are Cruciferae, Dipsacus asper, Imperata chinensis, Scutellaria baicalensis and Fagopyrum truncatum), comprising:

[0069] S1. Determination of the configuration type of seed embryo and morphological characteristics of endosperm: Collect the fruits of plants during the fruiting period, at least 10 plants; then remove the pulp of the fruit, remove impurities and dry it, and select plump seeds; embed the plant seeds in vermiculite with a water content of 20% for 24 hours, and control the vermiculite temperature at 25°C. After the embryo, endosperm and other structures inside the seed fully absorb water and expand, use a scalpel and a dissecting needle to peel off the seed embryo, endosperm and seed coat in sequence under a body dissecting microscope.

[0070] The two-dimensional configuration and morphology of the embryo and endosperm are observed under a stereoscopic dissecting microscope. If the plant seed satisfies the embryo configuration category of any one of subtype I (spoon-shaped, straight, lateral-based, stacked, peripheral or filled types), and the endosperm is missing / degenerated (thin), it is a rapid seed germination type plant.

[0071] In order to verify the accuracy of the above judgment, verification tests were conducted on the determined fast-germination plants, as follows:

[0072] S21. Rinse several plant seeds three times with water at about 25°C to remove surface stains and set aside.

[0073] S22. Take 12 plastic culture dishes with a diameter of 5.5 cm and a thickness of 0.5 cm, evenly place 50 plant seeds in each culture dish, and then add 5 ml of distilled water to each culture dish for later use.

[0074] S23. Four intelligent light incubators are used to simulate the environments of early spring and late autumn, late spring and early autumn, summer and winter respectively; the temperature in the light incubator used to simulate early spring and late autumn is 15℃ / 8℃ (i.e., the temperature of simulating 12 hours of light during the day is 15℃, and the temperature of simulating 12 hours of no light at night is 8℃), the temperature in the light incubator used to simulate summer is 25℃ / 18℃ (i.e., the temperature of simulating 12 hours of light during the day is 25℃, and the temperature of simulating 12 hours of no light at night is 18℃), the temperature in the light incubator used to simulate late spring and early autumn is 20℃ / 13℃ (i.e., the temperature of simulating 12 hours of light during the day is 20℃, and the temperature of simulating 12 hours of no light at night is 13℃), and the temperature in the light incubator used to simulate winter is 10℃ / 4℃ (i.e., the temperature of simulating 12 hours of light during the day is 10℃, and the temperature of simulating 4 hours of no light at night is 13℃). Three culture dishes containing plant seeds were set in each light incubator, and the seeds were cultured continuously. In each light incubator, the germination time of each germinated seed was recorded from the time the first seed germinated until no seed germinated. The germination rate of plant seeds in each light incubator was observed, and the season simulated by the light incubator with the highest germination rate was determined as the most suitable season for plant seed germination. The germination time of plant seeds in the light incubator with the highest germination rate was used as the horizontal coordinate and the germination rate was used as the vertical coordinate. The nonlinear iterative regression method was used to fit the S-shaped curve, and the fitting equation was y=a / (1+(x / x0) b ), where a represents the highest germination rate, x represents the time after the start of the experiment, x0 represents the median germination time, b represents the curve fitting parameter, and y represents the cumulative germination rate corresponding to x; thereby obtaining the median germination time of plant seeds, and verifying whether the seeds are fast-germinating plants based on the size of the median germination time. If the median germination time of the seeds is less than 30 days, it is verified as a fast-germinating plant, and if the median germination time is ≥30 days, it is verified as a non-fast-germinating plant (refer to CN 113341075 A for this method).

[0075] The test results are shown in Table 1 below.

[0076] Table 1 Example 1 Test results

[0077]

[0078] Note: ∞: positive infinity.

[0079] The results in Table 1 above show that the embryo configuration of the seeds of the above five herbaceous plants, Cruciferae, is head-shaped, so it is determined to be a non-rapid germination type plant. The endosperms of the seeds of Dipsacus asper and Herba Corydalis among the remaining four plants are thick, so the seeds of the above three plants are determined to be non-rapid germination type plants. The endosperms of the seeds of Herba Corydalis and Fagopyrum are degenerated, so they are determined to be fast germination type plants.

[0080] The seeds of the above five plants were tested for germination, and it was found that the median germination time of the five plants in the most suitable season was 39.21, ∞, 19.84, ∞, and 18.68 days, respectively. Therefore, it can be verified that golden grass and buckwheat are fast-germinating herbaceous plants.

[0081] Example 2

[0082] This example analyzes and determines the embryo configuration category and endosperm morphological characteristics of the seeds of the above 9 shrubs (Buddaria officinalis, Glechoma longituba, Viburnum chinense, Pyracantha fortuneana, Rosa microcarpa, Rosa rubus, Bulbous sorghum and Spiraea truncatum). The specific implementation method is the same as that of Example 1.

[0083] The test results are shown in Table 2 below.

[0084] Table 2 Example 2 Test results

[0085] Note: ∞: positive infinity.

[0086] The results in Table 2 above show that among the above 9 shrubs, the embryo configuration of the seeds of Glechoma longituba is cellular, so it is determined to be a non-rapid germination type plant. Among the remaining 8 plants, the endosperms of Viburnum chinense, Rosa microcarpa, and Rosa rubus are thick, and the endosperm of Sorghum kaobao seeds is lignified, so the seeds of the above 4 plants are determined to be non-rapid germination type plants. The endosperms of Buddaria japonica and Pyracantha fortuneana seeds are degenerated, and the endosperms of Rhizoma chinensis and Spiraea sphenanthera seeds are degenerated, so the above 4 plants are determined to be fast germination type plants.

[0087] The seeds of the above nine plants were tested for germination, and it was found that the median germination time of the nine plants in the most suitable season was 23.86, 37.33, ∞, 10.14, 10.31, ∞, ∞, ∞, and 9.85 days, respectively. Therefore, it can be verified that Buddaria japonica, Rhizoma Cibotii, Pyracantha fortuneana and Spiraea sphenanthera are shrubs with fast seed germination.

[0088] Example 3

[0089] This example is aimed at the above 6 shrubs / small trees (Whitethorn, Rhamnus serrata, Zanthoxylum bungeana, Rhamnoides yunnanensis, Sorbus serrata and Rhododendron chinense) and 2 vines (Cephalotaxus orbiculatus and Cistanche deserticola), a total of 8 plant seeds, and analyzes and determines the embryo configuration category and endosperm morphological characteristics in turn. The specific implementation method is the same as in Example 1.

[0090] The test results are shown in Table 3 below.

[0091] Table 3 Example 3 test results

[0092]

[0093] Note: ∞: positive infinity.

[0094] The results in Table 3 above show that among the above 8 shrubs / small trees and vines, the configuration category of the seed embryo of Rhododendron chinense is fusiform, so it is determined to be a non-rapid germination type plant. Among the remaining 7 plants, the endosperm of Nitraria serrata and Rhamnus slenderis is lignified, and the endosperm of Zanthoxylum baicalensis, Rhizoma yunnanensis and Celastrus orbiculatus is hypertrophic, so the seeds of the above 5 plants are determined to be non-rapid germination type plants. The endosperm of Sorbus serrata and Rhizoma serrata seeds is degenerated, so they are determined to be fast germination type plants.

[0095] The seeds of the above eight plants were tested for germination, and it was found that the median germination time of the eight plants in the most suitable season was 59.06, 52.58, ∞, ∞, 17.81, ∞, ∞, and 16.55 days, respectively. Therefore, it can be verified that Sorbus australis and Scutellaria baicalensis are fast-germinating shrubs / small trees and vines.

[0096] Example 4

[0097] In this embodiment, the embryo configuration category and endosperm morphological characteristics of the above six tree species (Alnus truncatula, Carpinus fusca, Catalpa ovata, Cypress truncatula and Toona sinensis) were analyzed and determined in turn. The test results are shown in Table 4 below.

[0098] Table 4 Example 4 Test results

[0099] Note: ∞: positive infinity.

[0100] The results in Table 4 above show that among the above 6 tree species, the endosperms of Quercus yunnanensis and Catalpa yunnanensis are lignified, and the endosperm of Toona sinensis is hypertrophic, so the seeds of the above 3 plants are determined to be non-rapid germination plants. The endosperms of Alnus truncatula, Catalpa ovata, and Cypress yunnanensis seeds are degenerated, so they are determined to be fast germination plants.

[0101] The germination experiments of the above six plant seeds were conducted and the median germination time of the six plants in the most suitable season was found to be 7.66, ∞, 65.01, 18.76, 16.86, and ∞ days, respectively. Therefore, it can be verified that Alnus, Catalpa, and Cypress are fast-germinating tree plants.

[0102] Figure 3 These are some photos of the embryo configuration and endosperm morphological characteristics of karst plant seeds. The black or white dotted lines in the figure are the whole embryo. Among them, a, Crossgrass, embryo head type, endosperm hypertrophy; b, Water redwood, embryo spindle type, endosperm hypertrophy; c, Yunnan cypress, embryo straight type, thin endosperm, degenerate; d, Sichuan teasel, embryo straight type, endosperm hypertrophy; e, Yunnan and Guizhou hornbeam, embryo spoon type, endosperm lignification; f, Spiraea, embryo spoon type, no endosperm; g, Alnus, embryo spoon type, thin endosperm, degenerate; h, Zanthoxylum baicalensis, embryo spoon type, endosperm hypertrophy; i, Yellow back grass, embryo lateral base type, endosperm hypertrophy.

[0103] By comparing the method provided in this application with the above-mentioned verification method, it can be found that the present application only takes 2 days to complete the judgment, while the above-mentioned verification method requires twice the median germination time, which generally takes dozens of days.

[0104] Compared with the verification method, the determination method provided in the present application is simple to operate and has a fast determination speed.

[0105] The present application uses a simple and easy biological analysis method to accurately and universally determine whether a plant is a fast-germinating plant. The determination method is used to screen out fast-germinating plants from a variety of plant seeds for use in degraded karst ecological restoration. The screened plants are highly representative, their seeds are easy to germinate and have a fast germination speed, and they are highly adaptable to environments with relatively scarce water resources. This can greatly improve the success rate of degraded karst ecological restoration based mainly on plant seeds.

[0106] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present application, rather than to limit it. Although the present application has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or replace some or all of the technical features therein with equivalents. However, these modifications or replacements do not cause the essence of the corresponding technical solutions to deviate from the scope of the technical solutions of the embodiments of the present application.

[0107] In addition, those skilled in the art will appreciate that, although some embodiments herein include certain features included in other embodiments but not other features, the combination of features of different embodiments is meant to be within the scope of the present application and form different embodiments. For example, in the above claims, any one of the claimed embodiments may be used in any combination. The information disclosed in this background technology section is intended only to deepen the understanding of the overall background technology of the present application and should not be regarded as an admission or in any form of implication that the information constitutes prior art known to those skilled in the art.

Claims

1. A method for determining a seed-rapid germination type karst plant, characterized in that: include: The seeds of the karst plants to be identified are pretreated, the pretreatment comprising: embedding the seeds in water-containing vermiculite, and then peeling off the seed coat, endosperm and embryo in sequence; the water content of the vermiculite is 15-20wt%, and the particle size of the vermiculite is 1-3mm; the embedding temperature is 20-25°C, and the time is 12-24h; The configuration category of the embryo and the morphological characteristics of the endosperm of the seeds of the karst plant to be identified are analyzed in sequence. If the configuration category of the embryo of the seeds meets the target configuration subtype I and the endosperm is missing or degenerated, the karst plant to be identified is determined to be a rapid germination type; otherwise, the karst plant to be identified is determined to be a non-rapid germination type; The target configuration subtype I includes any one of spoon type, straight type, side base type, stacked type, peripheral type or filled type; Spoon-shaped: The end of the embryonic root is upright and protruding, and the embryo as a whole is shaped like a short spoon; Straight type: The embryo is upright, and the length of the embryo is greater than its width; Lateral type: The embryo is located on one side of the seed base and extends toward the top along the edge of the seed coat; Peripheral type: The embryo is long and curved, and the embryo extends along the seed coat; Overlapping type: The embryo is short-spoon-shaped as a whole, but the embryo axis is folded; Filling type: The radicle end is short and upright, and the embryo fills the inside of the seed without a fine cavity; If the configuration category of the embryo of the seed is subtype II, the karst plant to be identified is determined to be a non-rapid germination type; The subtype II includes any one of the head type, cell type, ball type or spindle type; Head shape: The embryo is enlarged and head-shaped, located at the base of the seed; Cell type: The embryonic morphology is difficult to distinguish, and it is a single cell or a cell cluster; Spherical: The embryo is spherical and located at the base of the seed; Fusiform: The embryo is fusiform and located at the base of the seed; If the endosperm of the seed is thick or lignified, the karst plant to be identified is determined to be a non-rapid germination type; The method for collecting seeds of the karst plant to be determined comprises: collecting the fruit of the plant during the fruiting period of the plant, then removing the pulp of the fruit, removing impurities and drying the fruit, and selecting plump seeds; When performing the collection, the number of plants collected is not less than 10.

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