Imaging assembly carrier device, base station and microscopic image acquisition device and adjustment method

Abstract

The imaging assembly bearing device, base station and microscopic image acquisition device and adjusting method comprise an imaging lens support, an imaging lens clamping seat and an adjusting guide seat; the bottom of the imaging lens clamping seat is movably connected between the imaging lens support and the adjusting guide seat; the levelness of the upper surface of the bottom of the imaging lens clamping seat relative to the bottom of the imaging lens support can be adjusted until the microscopic imaging assembly fixed on the imaging lens clamping seat is perpendicular to the upper surface of the bottom of the imaging lens clamping seat. With the structural design of the imaging assembly bearing device and the combination of the screw fastener, the distance adjusting fastener can improve the distance adjustment accuracy, the angle adjustment of the angle α between the imaging assembly and the ideal optical axis is more accurate, the distance L2 in the optical axis direction is more accurate, and the high-precision optical adjustment process is realized.

Description

Technical Field

[0001] This application belongs to the field of microscopic image acquisition technology, and particularly relates to an imaging component support device, a base for supporting a microscopic image acquisition device, and a microscopic imaging acquisition device disposed on the imaging component support base. Background Technology

[0002] In existing technologies, microscope imaging typically requires manual adjustment of the microscope's focus to obtain a clear target image. However, in microscopic imaging using an improved automated focusing system with an electronic control system, the focus adjustment process is extremely precise due to the varying magnification of the microscope. This demands high precision not only from the power components but also from the mechanical support mechanisms that carry the power components and key imaging components.

[0003] When the magnification is 40x, the allowable deviation in the depth of field (the distance between the objective lens and the target) is approximately ±0.25 μm. At this depth, the resolution of the distance adjustment between the objective lens and the target needs to be on the same order of magnitude as the depth of field to ensure reliable adjustment during the focusing process in electronically controlled microscopy. In other words, if the microscope's depth of field ranges from 2 μm to 3 μm, the movement precision of the electronically controlled mechanical platform supporting the microscope's movement must also be within 2 μm to 3 μm to ensure that the microscopic imaging device can stably obtain clear microscopic images during movement.

[0004] The movement of the electromechanical platform supporting the microscope requires a planar accuracy of 2 to 3 μm. This places high demands on both the precision of the motor's drive control and the accuracy of the microscope's base. To achieve appropriate magnification, microscopes typically employ a lens assembly after the objective lens for light adjustment. This assembly, usually cylindrical, needs to be precisely orthogonal to the imaging target. Even a slight deviation in the angle between the central optical axis and the plane of the imaging target can lead to a significant deviation in the distance between the target and the focal point. The longer the optical axis of the lens assembly, the greater the effect of non-orthogonality between the central optical axis and the plane of the imaging target, resulting in blurry images and ghosting in the microscopic image.

[0005] However, in existing technologies, the required machining accuracy and the machining cost of mechanical components exhibit an exponential growth relationship; that is, the machining cost of high-precision mechanical planes is far greater than that of general-precision planes. How to achieve high-precision application requirements using general-precision mechanical planes is one of the key technical problems this application aims to solve.

[0006] Even high-precision components inevitably have some dimensional errors in their manufacturing process. How to ensure that the central optical axis and the plane of the imaging target are as close as possible to orthogonal in microscopic imaging when there are dimensional errors in the components is one of the key technical problems that this application aims to solve. Summary of the Invention

[0007] The technical problem to be solved by this application is to avoid the shortcomings of the prior art, where the central optical axis and the plane where the imaging target is located may not be orthogonal. It proposes an imaging component support device, a base, and a microscopic imaging acquisition device set on the imaging component support base.

[0008] The technical solution of this application to solve the above problems is an imaging component carrying device for carrying imaging components in a microscopic imaging acquisition device; it includes an imaging lens support, an imaging lens holder, and an adjustment guide seat; the adjustment guide seat is fixedly connected to the imaging lens support; the imaging lens holder includes an imaging lens holder bottom and a lens holding part; the imaging lens holder lens through hole passes through the imaging lens holder bottom and the lens holding part; the imaging lens support is provided with a support lens through hole; the adjustment guide seat is provided with a guide seat through hole; the lens holding part passes through the guide seat through hole on the adjustment guide seat, so that the bottom of the imaging lens holder is movably engaged between the imaging lens support and the adjustment guide seat; when the bottom of the imaging lens holder is engaged between the imaging lens support and the adjustment guide seat, the positions of the support lens through hole and the imaging lens holder lens through hole correspond.

[0009] The imaging lens holder includes an imaging lens holder bottom; a lens through hole is provided on the bottom of the imaging lens holder; at least three first elastic support members are provided on the bottom of the imaging lens holder, and the plurality of first elastic support members are evenly distributed around the lens through hole of the holder; the elastic support surface or elastic support point of the first elastic support member faces upward to support the bottom of the imaging lens holder.

[0010] The guide seat is provided with at least three limit position adjustment holes, and limit distance adjustment fasteners are provided in the limit position adjustment holes; the position of each limit position adjustment hole corresponds to the position of each first elastic support.

[0011] The limit position adjustment hole is a limit position adjustment through hole; the limit distance adjustment fastener passes through the limit position adjustment through hole and contacts the bottom of the imaging lens holder.

[0012] The limit distance adjusting fastener is a helical fastener. The downward distance of the helical fastener is controlled by the rotation angle. The lead S is the downward distance of the helical fastener for one revolution of the thread. The range of the lead S of the helical fastener is 0.2mm to 1mm.

[0013] On the adjusting guide seat, two position fixing through holes A1 are symmetrically arranged on both sides of each limiting position adjusting hole; correspondingly, two position fixing through holes B1 are also provided on the bottom of the imaging lens holder; each position fixing fastener passes through the corresponding fixing through hole A1 and fixing through hole B1 in sequence to fix the relative position between the bottom of the adjusting guide seat and the imaging lens holder.

[0014] When the bottom of the imaging lens holder is pressed against the first elastic support, the downward distance of the first elastic support ranges from 0.3 mm to 2 mm.

[0015] The adjusting guide seat is provided with at least three second elastic support members for radial support of the bottom of the imaging lens holder; the second elastic support members are evenly distributed around the geometric center of the adjusting guide seat and are radially arranged around the lens through hole of the imaging lens holder; the elastic support surface or elastic support point of the second elastic support member faces the radial center of the lens through hole of the imaging lens holder and is used to contact the side wall of the bottom of the imaging lens holder.

[0016] The imaging component support device further includes a motor bracket and a movable plate; the motor bracket is used to fix the motor that drives the imaging component to move; the movable plate is used to connect to the driving force output device of the motor; the imaging lens bracket and the movable plate are fixedly connected, and the imaging lens bracket obtains the moving power by means of the movable plate.

[0017] The imaging component support device further includes a motor body and a driving force output device; the motor body is fixed on a motor bracket; the rotation shaft of the motor body is connected to the driving force output device; the driving force output device is connected to a movable plate, driving the movable plate to move.

[0018] The imaging lens bracket also includes an imaging lens bracket vertical surface and two imaging lens bracket support half-side surfaces; the imaging lens bracket is fixedly connected to the imaging lens bracket vertical surface and the movable plate, so that the imaging lens bracket can move with the movable plate; the bottom of the imaging lens bracket and the imaging lens bracket vertical surface are fixedly connected to the imaging lens bracket support half-side surfaces through two imaging lens bracket support half-side surfaces.

[0019] The technical solution of this application to solve the above problems can also be an imaging component support base for supporting a microscopic imaging acquisition device, including the above-mentioned imaging component support device; it also includes a support substrate, a first support bracket, a second support bracket, and an imaging target clamping assembly; the first support bracket and the second support bracket are respectively disposed on the support substrate; the first support bracket is used to support the imaging component support device; the second support bracket is used to support the imaging target clamping assembly, so that the imaging target clamping assembly is disposed below the imaging component support device; the first support bracket includes a longitudinal support column and a transverse support arm, one end of the longitudinal support column and the transverse support arm are fixedly connected, and a transverse support arm fixing through hole is provided on the outward extension of the transverse support arm, and the imaging component support device is fixed on the transverse support arm by means of the transverse support arm fixing through hole; the second support bracket is provided with a second support through hole, and the center position of the transverse support arm fixing through hole and the center position of the second support through hole are relatively fixed.

[0020] The outward extension of the lateral support arm is connected to the longitudinal support column by reinforced corner brackets.

[0021] The bottom of the supporting base plate is equipped with multiple shock-absorbing and damping feet.

[0022] The imaging target clamping assembly includes an XY-axis moving platform assembly and a reagent card holder assembly; the reagent card holder assembly is fixed to the top of the XY-axis moving platform assembly; the XY-axis moving platform assembly is fixed to the top of the second support bracket.

[0023] The reagent card holder assembly includes a reagent card holder body and a reagent card holding part; the reagent card holding part is used to hold externally inserted reagent cards; the bottom surface of the reagent card holder body and the bottom surface of the reagent card holding part are parallel.

[0024] The second support bracket is equipped with multiple third elastic support components for adjusting the levelness of the bottom surface of the XY axis moving platform assembly.

[0025] Multiple fourth elastic supports are provided on the top surface of the XY axis moving platform assembly for adjusting the levelness of the bottom surface of the reagent card holder assembly.

[0026] The imaging component support base also includes a light source component; a light source fixing frustum is provided on the second support bracket with the second support through hole as the center; the light source component is fixed on the light source fixing frustum; so that the light emitted from the light source in the light source component and the imaging lens in the imaging component support device are on the same imaging optical axis.

[0027] The technical solution of this application to solve the above problems can also be a microscopic image acquisition device, including the above-mentioned imaging component support device or the above-mentioned imaging component support base; it also includes a microscopic imaging component, which is fixed on an imaging lens holder.

[0028] The technical solution of this application to solve the above problems can also be an adjustment method for a microscopic image acquisition device, based on the above-mentioned imaging component support device; and a microscopic imaging component fixed on an imaging lens holder; the microscopic imaging component includes an objective lens; the objective lens passes through the imaging lens holder, the objective lens is clamped and fixed by the imaging lens holder, and one end of the objective lens is located below the plane where the bottom of the imaging lens holder is located; the target imaging optical axis passes through the objective lens; including the following steps: Step A: Obtain the offset direction between the lens through hole of the imaging lens holder and the target imaging optical axis, if the plane where the lens through hole of the imaging lens holder is located and the target imaging optical axis are offset by the offset direction between the lens through hole of the imaging lens holder and the target imaging optical axis, the offset direction between the lens through hole of the imaging lens holder and the target imaging optical axis is adjusted accordingly. If the offset angle is greater than the set target value, proceed to step B; Step B: Select the limiting distance adjusting fastener in the limiting position adjusting hole closest to the end of the objective lens, adjust the depth of the limiting distance adjusting fastener as it passes downward, and adjust the force of the bottom of the imaging lens holder pressing the corresponding first elastic support at that point; Step C: Check again the offset angle between the plane of the imaging lens holder's lens through hole and the target imaging optical axis. If the offset angle is greater than the set target value, return to step B; until the offset angle between the plane of the imaging lens holder's lens through hole and the target imaging optical axis is less than or equal to the set target value.

[0029] The adjustment method of the microscopic image acquisition device further includes step D: when the offset angle between the plane of the lens through hole of the imaging lens holder and the target imaging optical axis is less than or equal to the set target value, the bottom of the imaging lens holder and the adjustment guide seat are fixedly connected.

[0030] Compared with the prior art, one of the beneficial effects of this application is that the bottom of the imaging lens holder is movably engaged between the imaging lens support and the adjustment guide seat; this provides an adjustable space between the bottom of the imaging lens holder and the imaging lens support, and also allows the imaging components, especially the lens components, clamped on the imaging lens holder to have the opportunity to adjust the perpendicularity of the optical axis.

[0031] Compared with the prior art, the second beneficial effect of this application is that the three first elastic support members are evenly distributed around the through hole of the support lens, with their elastic support surfaces or elastic support points facing upwards, supporting the bottom of the imaging lens holder. The support surface supported by the three points is movable and adjustable, and the level of the support surface can be adjusted by adjusting the pressure of the three points to offset the level deviation of the support surface caused by the processing error of the related mechanical parts.

[0032] Compared with the prior art, the third beneficial technical effect of this application is that the limit distance adjusting fastener can directly adjust the pressing force at a single point, providing a more refined adjustment means for adjusting the level deviation of the support surface. The fastener can be a high-precision limit distance adjusting fastener.

[0033] Compared with the prior art, the fourth beneficial technical effect of this application is that the limiting distance adjusting fastener passes through the limiting position adjusting through hole and directly contacts the upper surface of the bottom of the imaging lens holder to adjust the pressure of the adjusting guide seat pressing on the corresponding first elastic support, thereby adjusting the downward distance of the bottom of the imaging lens holder pressing on the first elastic support.

[0034] Compared with the prior art, the fifth beneficial technical effect of this application is that the position fixing fasteners pass through the corresponding fixing through holes A1 and B1 in sequence to fix the relative position between the bottom of the adjusting guide seat and the bottom of the imaging lens clamping seat; that is, after each of the first elastic support members is adjusted into place, the relative position between the bottom of the adjusting guide seat and the bottom of the imaging lens clamping seat can be fixed.

[0035] Compared with the prior art, the sixth beneficial effect of this application is that when the bottom of the imaging lens holder is pressed against the first elastic support, the downward distance of the first elastic support ranges from 0.3 mm to 2 mm. This distance is sufficient to offset the deviation of the support surface level caused by the processing error of the relevant mechanical parts.

[0036] Compared with the prior art, the seventh beneficial effect of this application is that at least three second elastic support members are provided on the adjusting guide seat to ensure the centering of the imaging lens holder and reduce the friction between the adjusting guide seat and the imaging lens holder.

[0037] Compared with the prior art, the eighth beneficial technical effect of this application is that the movable plate enables the imaging lens holder to obtain the power for movement. The movable plate can drive the imaging lens holder to move longitudinally along the optical axis.

[0038] Compared with the prior art, the ninth beneficial technical effect of this application is that the vertical surface of the imaging lens holder and the two imaging lens holder support half-side surface make the bottom of the imaging lens holder more stably support the imaging lens clamp and adjustment guide seat.

[0039] Compared with the prior art, the tenth beneficial technical effect of this application is that the center position of the fixed through hole of the lateral support arm and the center position of the second support through hole are relatively fixed, which provides a basis for the coaxiality of the corresponding optical components in the subsequent light source assembly, imaging target clamping assembly, and imaging assembly carrying device.

[0040] Compared with the prior art, the eleventh beneficial technical effect of this application is that multiple shock-damping legs further enhance the structural stability and robustness of the imaging component support base, and reduce the impact of external vibration on the imaging component support base.

[0041] Compared with the prior art, the twelfth beneficial technical effect of this application is that the bottom surface of the reagent card holder body and the bottom surface of the reagent card holder are parallel, so that the reagent card can be stably held by the reagent card holder and can be orthogonal to the imaging optical axis.

[0042] Compared with the prior art, the thirteenth beneficial technical effect of this application is that the XY axis moving platform component can drive the reagent card holder component to move horizontally in two dimensions, which increases the flexibility of the system and facilitates the adjustment of the imaging target area.

[0043] Compared with the prior art, the fourteenth beneficial technical effect of this application is that the third elastic support can partially offset the assembly error between the bottom surface of the XY-axis moving platform assembly and the top surface of the second bearing bracket, and can adjust the levelness of the bottom surface of the XY-axis moving platform assembly. The levelness of the bottom surface of the XY-axis moving platform assembly is a levelness relative to the optical axis when it is orthogonal.

[0044] Compared with the prior art, the fifteenth beneficial technical effect of this application, the fourth elastic support, can partially offset the assembly error between the top surface of the XY-axis moving platform assembly and the bottom surface of the reagent card holder assembly, and can adjust the levelness of the bottom surface of the reagent card holder assembly. The levelness of the bottom surface of the reagent card holder assembly is a levelness relative to the optical axis when it is orthogonal.

[0045] Compared with existing technologies, the sixteenth beneficial effect of this application is that, through the structural design of the imaging component support device and the combination of spiral fasteners, the distance adjustment fasteners can improve the accuracy of distance adjustment, making the adjustment of the angle α between the imaging component and the ideal optical axis more precise, and the adjustment of the movement distance L2 in the optical axis direction more precise. In balancing component processing accuracy and cost, it achieves high-precision dimensional adjustment with relatively low processing accuracy, providing an efficient and low-cost solution. High-precision position adjustment can be achieved with low-precision component processing dimensions; the millimeter-level distance adjustment between the bottom of the imaging lens holder and the adjustment guide seat can be converted into a more precise dimensional distance adjustment of the objective lens in the optical axis direction. In other words, the millimeter-sized distance adjustment between the bottom of the imaging lens holder and the adjustment guide seat can be converted into a more precise distance adjustment of the objective lens in the optical axis direction; the lens in the imaging component carrier and its imaging optical axis can be easily adjusted to a position orthogonal to the plane of the lens through hole of the imaging lens holder, thereby ensuring that when imaging the target area, the imaging optical axis is orthogonal to the reagent card held on the reagent card holder assembly, thus ensuring the high quality of the microscopic imaging image. Attached Figure Description

[0046] Figure 1 This is a schematic diagram of the combined state of one of the 200 embodiments of the imaging component carrier device;

[0047] Figure 2 This is an exploded view of one of the 200 embodiments of the imaging component carrier device;

[0048] Figure 3 This is a bottom view of the combined state of the imaging lens holder 260 and the adjustment guide seat 270;

[0049] Figure 4 This is a schematic diagram of the combined state of an embodiment of the imaging component carrier device 200;

[0050] Figure 5 This is one of the exploded state schematic diagrams of a second embodiment of the imaging component carrier device 200;

[0051] Figure 6 This is the second exploded state schematic diagram of the second embodiment of the imaging component carrier device 200;

[0052] Figure 7 This is a top-view orthographic projection schematic diagram of a second embodiment of the imaging component carrier device 200;

[0053] Figure 8 yes Figure 7 AA sectional view;

[0054] Figure 9 This is a schematic diagram of the combined state of one embodiment of the microscopic image acquisition device;

[0055] Figure 10 This is a schematic diagram of the combined state of one embodiment of the imaging component carrier base;

[0056] Figure 11 This is a schematic diagram showing the disassembled state of the imaging component's support platform and the light source component;

[0057] Figure 12 This is a schematic diagram of the disassembled state of the imaging target clamping component 300;

[0058] Figure 13 This is a schematic diagram of the reagent card holder assembly 320;

[0059] Figure 14 This is a schematic diagram of the disassembled state of the reagent card holder assembly 320;

[0060] Figure 15 This is a schematic diagram of the XY-axis moving platform component 310;

[0061] Figure 16 This is a schematic diagram of the exploded state of the XY axis moving platform component 310;

[0062] Figure 17 This is a schematic diagram of the combined state of the imaging lens holder 260, the adjustment guide seat 270, and the bottom of the imaging lens support 232;

[0063] Figure 18 This is a top view of the combined state of the imaging lens holder 260, the adjustment guide seat 270, and the bottom of the imaging lens support 232;

[0064] Figure 19 yes Figure 18 BB cross-sectional schematic diagram; F in the figure represents the contact surface F between the lower surface of the bottom 261 of the imaging lens holder and the first elastic support 233; G is the schematic diagram of the imaging optical axis; in an ideal state, the imaging optical axis G is perpendicular to the contact surface F, which means that the imaging optical axis and the imaging target area are orthogonal, and the plane image where the target imaging area is located can be normally magnified by microscopy, the image is clear, and there is no ghosting.

[0065] Figure 20 yes Figure 19 One of the enlarged schematic diagrams of part K in the middle; H1 in the figure is the gap between the lower surface of the bottom 261 of the imaging lens holder and the upper surface of the bottom 232 of the imaging lens support;

[0066] Figure 21 yes Figure 20 Part 2 of the enlarged schematic diagram of section K; H2 in the diagram is the gap between the lower surface of the bottom 261 of the imaging lens holder and the upper surface of the bottom 232 of the imaging lens support; gap H2 is smaller than gap H1;

[0067] Figure 22 yes Figure 19 One of the simplified schematic diagrams of the principle;

[0068] Figure 23 yes Figure 19 The second simplified schematic diagram of the principle. Detailed Implementation

[0069] The embodiments of this application will be further described in detail below with reference to the accompanying drawings.

[0070] It should be noted that the following description of preferred embodiments of this application does not constitute any limitation on this application. The description of preferred embodiments is merely an illustration of the general principles of this application. The embodiments described in this application are only some embodiments of this application, and not all embodiments. Based on the embodiments in this application, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of this application.

[0071] In the description of this application, it should be understood that the terms "center," "longitudinal," "lateral," "length," "width," "thickness," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," "clockwise," and "counterclockwise," etc., indicating orientation or positional relationships based on the orientation or positional relationships shown in the accompanying drawings, are only for the convenience of describing this application and simplifying the description, and do not indicate or imply that the equipment or components referred to must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation on this application. Furthermore, the terms "first," "second," and technical features numbered with Arabic numerals 1, 2, 3, etc., and designations such as "A" and "B," are used for descriptive purposes only, for the convenience of explanation, and do not represent a temporal or spatial order; they should not be construed as indicating or implying relative importance or implicitly specifying the number of technical features indicated. Therefore, features defined with "first," "second," and numbered with Arabic numerals 1, 2, 3, etc., may explicitly or implicitly include one or more of that feature. In the description of this application, "several" means two or more, unless otherwise expressly and specifically defined.

[0072] like Figures 1 to 2 As shown, in one embodiment of an imaging component carrying device 200, there are an imaging lens support 230, an imaging lens holder 260, and an adjustment guide seat 270. The adjustment guide seat 270 is fixedly connected to the imaging lens support 230. The imaging lens holder 260 includes an imaging lens holder bottom 261 and a lens holding portion 262. The imaging lens holder lens through hole 266 passes through the imaging lens holder bottom 261 and the lens holding portion 262. The imaging lens support 230 is provided with a support lens through hole 231. The adjustment guide seat 270 is provided with a guide seat through hole 279. The lens holding portion 262 passes through the guide seat through hole 279 on the adjustment guide seat 270, so that the imaging lens holder bottom 261 is movably engaged between the imaging lens support 230 and the adjustment guide seat 270. When the imaging lens holder bottom 261 is engaged between the imaging lens support and the adjustment guide seat, the positions of the support lens through hole 231 and the imaging lens holder lens through hole 266 correspond.

[0073] like Figures 1 to 3In the embodiment of the imaging component support device 200 shown, the imaging lens holder 230 includes an imaging lens holder bottom 232; a holder lens through-hole 231 is disposed on the imaging lens holder bottom 232; at least three first elastic support members 233 are provided on the imaging lens holder bottom 232, and the plurality of first elastic support members 233 are evenly distributed around the holder lens through-hole 231; the elastic support surface or elastic support point of the first elastic support member 233 faces upward to support the bottom 261 of the imaging lens holder. A first elastic support member countersunk hole 234 is provided on the imaging lens holder bottom 232, the first elastic support member countersunk hole 234 is used to accommodate the first elastic support member 233, and the bottom end of the first elastic support member 233 falls into the first elastic support member countersunk hole 234 for support. The number and position of the first elastic support member countersunk holes 234 correspond one-to-one with the first elastic support member 233; the first elastic support member countersunk holes 234 are not through holes.

[0074] like Figures 1 to 3 In the embodiment of the imaging component carrier 200 shown, the adjusting guide seat 270 is provided with at least three limiting position adjusting holes 271, and a limiting distance adjusting fastener 272 is provided in each limiting position adjusting hole 271; the position of each limiting position adjusting hole 271 corresponds to the position of each first elastic support 233. By adjusting the distance the limiting distance adjusting fastener 272 penetrates into the limiting position adjusting hole 271, the pressure of the adjusting guide seat 270 pressing against the corresponding first elastic support 233 at that point is adjusted.

[0075] like Figures 1 to 3 In the embodiment of the imaging component carrier 200 shown, the limiting position adjustment hole 271 is a limiting position adjustment through hole; the limiting distance adjustment fastener 272 passes through the limiting position adjustment through hole and contacts the bottom 261 of the imaging lens holder. The limiting distance adjustment fastener 272 passes through the limiting position adjustment through hole 271 and directly contacts the upper surface of the bottom 261 of the imaging lens holder to adjust the pressure of the adjusting guide seat 270 pressing on the corresponding first elastic support 233, thereby adjusting the downward distance of the bottom 261 of the imaging lens holder pressing on the first elastic support 233.

[0076] like Figures 1 to 2In the embodiment of the imaging component carrier 200 shown, two position fixing through holes A1275 are symmetrically arranged on both sides of each limiting position adjustment hole 271 on the adjusting guide seat 270; correspondingly, two position fixing through holes B1265 are also provided on the bottom 261 of the imaging lens holder; each position fixing fastener passes through the corresponding fixing through hole A1275 and fixing through hole B1265 in sequence to fix the relative position between the adjusting guide seat 270 and the bottom 261 of the imaging lens holder. The position fixing fasteners are not shown in the drawings. When the bottom 261 of the imaging lens holder is pressed against the first elastic support 233, the downward distance of the first elastic support 233 ranges from 0.3mm to 2mm or from 0.5mm to 1.2mm.

[0077] like Figures 1 to 3 In the embodiment of the imaging component carrier 200 shown, at least three second elastic support members 276 are provided on the adjusting guide seat 270 for radial support of the bottom 261 of the imaging lens holder. The second elastic support members 276 are evenly distributed around the geometric center of the adjusting guide seat 270 and radially arranged around the lens through hole 266 of the imaging lens holder. The elastic support surface or elastic support point of the second elastic support member 276 faces the radial center of the lens through hole 266 of the imaging lens holder and is used to contact the sidewall of the bottom 261 of the imaging lens holder. The at least three second elastic support members 276 provided on the adjusting guide seat 270 ensure the centering of the imaging lens holder 260 and reduce the friction between the adjusting guide seat 270 and the imaging lens holder 260.

[0078] like Figures 1 to 4 In the embodiment of the imaging component carrier 200 shown, the imaging lens bracket 230 further includes an imaging lens bracket vertical surface 235 and two imaging lens bracket support half-side surfaces 236; the imaging lens bracket 230 is fixedly connected to the movable plate 220 through the imaging lens bracket vertical surface 235, so that the imaging lens bracket 230 can move with the movable plate 220; the bottom 232 of the imaging lens bracket and the imaging lens bracket vertical surface 235 are fixedly connected through the two imaging lens bracket support half-side surfaces 236.

[0079] like Figures 4 to 8In the embodiment of the imaging component support device 200 shown, it further includes a motor bracket 210, a movable plate 220, a motor body 280, and a driving force output device 290; the motor bracket 210 is used to fix the motor body 280 that drives the imaging component to move; the motor body 280 is fixed on the motor bracket 210; the rotation shaft of the motor body 280 is connected to the driving force output device 290; the driving force output device 290 is connected to the movable plate 220, driving the movable plate 220 to move. The movable plate 220 is used to connect with the driving force output device 290 of the motor body 280; the imaging lens bracket 230 is fixedly connected to the movable plate 220, and the imaging lens bracket 230 obtains the power to move by means of the movable plate 220.

[0080] like Figures 4 to 8 In the embodiment of the imaging component carrier 200 shown, the driving force output device 290 includes a bearing housing assembly 291, a ball screw nut 292, a ball screw nut fastening seat 293, and a ball screw 294; the bearing housing assembly 291 is sleeved on the rotating shaft of the motor, and the ball screw 294 is connected to the rotating shaft of the motor; the ball screw nut 292 is sleeved on the ball screw 294; the ball screw nut fastening seat 293 is connected to the ball screw nut 292, and the ball screw nut fastening seat 293 is fixedly connected to the movable plate 220 and moves with the movement of the ball screw nut 292.

[0081] like Figures 9 to 11 As shown, in one embodiment of an imaging component support base for a microscopic imaging acquisition device, the device includes an imaging component support device 200, a support substrate 120, a first support bracket 110, a second support bracket 130, and an imaging target clamping assembly 300. The first support bracket 110 and the second support bracket 130 are respectively disposed on the support substrate 120. The first support bracket 110 supports the imaging component support device 200. The second support bracket 130 supports the imaging target clamping assembly 300, such that the imaging target clamping assembly 300 is disposed below the imaging component support device 200. The bottom of the support substrate 120 is provided with a plurality of shock-absorbing damping feet 125.

[0082] like Figures 9 to 11As shown, in an embodiment of an imaging component support base for a microscopic imaging acquisition device, the first support bracket 110 includes a longitudinal support column 111 and a transverse support arm 112. One end of the longitudinal support column 111 and the transverse support arm 112 are fixedly connected. The outward extension portion 1122 of the transverse support arm is reinforcedly connected to the longitudinal support column 111 by a reinforcing bracket 113. A transverse support arm fixing through hole 1123 is provided on the outward extension portion 1122 of the transverse support arm, and the imaging component support device 200 is fixed to the transverse support arm 112 by means of the transverse support arm fixing through hole 1123. A second support bracket 130 is provided with a second support through hole 133, and the center position of the transverse support arm fixing through hole 1123 and the center position of the second support through hole 133 are relatively fixed. This is one of the measures to ensure the coaxiality of various optical components on the optical axis.

[0083] like Figures 12 to 16 As shown, in an embodiment of an imaging component support base for a microscopic imaging acquisition device, the imaging target clamping assembly 300 includes an XY-axis moving platform assembly 310 and a reagent card clamping base assembly 320. The reagent card clamping base assembly 320 is used to clamp an external reagent card 390. The reagent card clamping base assembly 320 is fixed to the top of the XY-axis moving platform assembly 310. The XY-axis moving platform assembly 310 is fixed to the top of the second support bracket 130. The reagent card clamping base assembly 320 includes a reagent card clamping base body 321 and a reagent card clamping part 322. The reagent card clamping part 322 is used to clamp an externally inserted reagent card 390. The bottom surface of the reagent card clamping base body 321 and the bottom surface of the reagent card clamping part 322 are parallel. The reagent card clamping base body 321 and the reagent card clamping part 322 can be integrally formed or combined; or they can be separate independent components. The reagent card holder body 321 is also provided with a plurality of elastic clamping support components 323. The reagent card clamping part 322 includes two reagent card clamping strips, and each reagent card clamping strip is provided with two elastic clamping support components 323. The external reagent card 390 is clamped between the reagent card clamping part 322 and the elastic clamping components 323.

[0084] like Figure 16 As shown, in an embodiment of an imaging component support platform for a microscopic imaging acquisition device, the XY-axis moving platform assembly 310 includes a moving platform base 311, a first moving component 312, a second moving component 313, a first drive motor 315, and a second drive motor 314. The first drive motor 315 is connected to the first moving component 312, driving the first moving component 312 to move horizontally. The second drive motor 314 is connected to the second moving component 313, driving the second moving component 313 to move horizontally. The moving directions of the first moving component 312 and the second moving component 313 are orthogonal, completing the two-dimensional movement of the XY-axis moving platform assembly 310 on the horizontal plane.

[0085] In one embodiment of an imaging component support base for a microscopic imaging acquisition device (not shown in the accompanying drawings), a second support bracket 130 is provided with multiple third elastic supports for adjusting the levelness of the bottom surface of the XY-axis moving platform assembly 310. A plurality of fourth elastic supports are provided on the top surface of the XY-axis moving platform assembly 310 for adjusting the levelness of the bottom surface of the reagent card holder assembly 320.

[0086] like Figures 9 to 11 As shown, in one embodiment of an imaging component support base for a microscopic imaging acquisition device, a light source component 500 is further included; a light source fixing frustum 136 is provided on the second support bracket 130 with the second support through hole 133 as the center; the light source component 500 is fixed on the light source fixing frustum 136; so that the light emitted from the light source in the light source component 500 and the imaging lens in the imaging component support device 200 are on the same imaging optical axis.

[0087] like Figure 3 , Figures 9 to 17 As shown, in one embodiment of a microscopic image acquisition device, there are imaging component support devices 200, imaging component support bases, and microscopic imaging components 600; the microscopic imaging components 600 are fixed on imaging lens holders 260. The microscopic imaging components 600 include an objective lens 610; the objective lens 610 passes through the imaging lens holders 260 and is held and fixed by the imaging lens holders 260, with one end of the objective lens 610 located below the plane of the bottom 261 of the imaging lens holder; the objective lens 610 is located on the target imaging optical axis; the bottom surface of the lens through-hole 266 of the imaging lens holder is the bottom through-hole 2661 of the imaging lens holder.

[0088] In an embodiment of an adjustment method for a microscopic image acquisition device (not shown in the accompanying drawings), the method includes the following steps: Step A: Obtain the offset direction between the bottom through hole 2661 of the imaging lens holder and the target imaging optical axis. If the offset angle between the plane containing the bottom through hole 2661 of the imaging lens holder and the target imaging optical axis is greater than a set target value, proceed to Step B; Step B: Select the limiting distance adjusting fastener 272 in the limiting position adjusting hole 271 closest to the end of the objective lens 610, adjust the downward penetration depth of the limiting distance adjusting fastener 272, and adjust the bottom 261 of the imaging lens holder at that point. Step C: Press the corresponding first elastic support 233 with force; Step D: Check again the offset angle between the plane where the bottom through hole 2661 of the imaging lens holder is located and the target imaging optical axis. If the offset angle is greater than the set target value, return to step B; until the offset angle between the plane where the bottom through hole 2661 of the imaging lens holder is located and the target imaging optical axis is less than or equal to the set target value; Step D: When the offset angle between the plane where the lens through hole 266 of the imaging lens holder is located and the target imaging optical axis is less than or equal to the set target value, make the bottom 261 of the imaging lens holder and the adjustment guide seat 270 complete the fixed connection.

[0089] like Figures 19 to 21 As shown, the imaging lens holder 260, the adjusting guide seat 270, and the bottom 232 of the imaging lens support are assembled together; the reference numeral F in the figure indicates the contact surface F between the lower surface of the bottom 261 of the imaging lens holder and the first elastic support 233; the reference numeral G is a schematic diagram of the imaging optical axis; in an ideal state, the imaging optical axis G is perpendicular to the contact surface F, which means that the imaging optical axis and the imaging target area are orthogonal, and the plane image where the target imaging area is located can be normally magnified by microscopy, the image is clear, and there is no ghosting. Figure 20 What is shown is a state. Figure 21 The image shows a state where the first elastic support 233 is pressed down more forcefully. Figure 20 H1 is the gap between the lower surface of the bottom 261 of the imaging lens holder and the upper surface of the bottom 232 of the imaging lens support; Figure 21 H2 is the gap between the lower surface of the bottom 261 of the imaging lens holder and the upper surface of the bottom 232 of the imaging lens support; gap H2 is smaller than gap H1.

[0090] like Figure 22 As shown, there is an angle α between the main body G1 of the microscopic imaging assembly 600 and the target imaging optical axis G0. At this time, the plane F1 where the bottom 261 of the imaging lens holder is located also has an angle α with the ideal contact surface F0. When this angle α is zero, as... Figure 23 As shown, ideally, the main body of the microscopic imaging component 600, i.e., the actual optical axis G1, coincides with the target imaging optical axis G0. At this time, the plane F1 where the bottom 261 of the imaging lens holder is located also coincides with the position of the ideal contact surface F0. Figure 23 In this diagram, to distinguish them, the actual optical axis G1 is not completely aligned with the target imaging optical axis G0, nor are the plane F1 containing the bottom 261 of the imaging lens holder and the ideal contact surface F0 aligned. However, the actual optical axis G1 is completely orthogonal to the plane F1 containing the bottom 261 of the imaging lens holder. Similarly, the target imaging optical axis G0 and the ideal contact surface F0 are also completely orthogonal, with point S being their intersection point.

[0091] Figure 22 and Figure 23 In this diagram, R1 is the distance from the first elastic support 233 to the center of the lens through-hole 266 of the imaging lens holder, i.e., the distance from the first elastic support 233 to the central optical axis; R2 is the distance from the front end of the objective lens 610 to the bottom 261 of the imaging lens holder; distance L1 is the distance the first elastic support 233 is pressed down; distance L2 is the distance the objective lens 610 moves on the target imaging optical axis G0 due to the adjustment of distance L1. tanα=L1 / R1; from this, the degree measure of angle α can be obtained, α=arctag(L1 / R1); based on this, L2=R2-L3=R2-R2×cosα can be calculated; usually, angle α is very small, and cosα is very close to 1, so the actual size of L2 is usually very small. If distance L2 is small enough, for example, L2 is less than or equal to the depth of field of the imaging, it generally will not have a substantial impact on the image quality of the microscopic imaging. When the α angle is too large, making the distance L2 greater than the depth of field of the image, it will have a substantial impact on the image quality of the microscopic imaging, resulting in ghosting or inability to focus clearly.

[0092] Therefore, the adjustment of angle α is one of the key influencing factors, and α = arctag(L1 / R1); since R1 is a fixed constant after the system structure is finalized, the adjustment accuracy of angle α is determined by the adjustment accuracy of L1.

[0093] L1 can be adjusted by the downward distance of the fastener. That is, the size of L1 can be controlled by the downward distance of the limit distance adjusting fastener 272. The higher the control precision of the downward distance of the limit distance adjusting fastener 272, the more precisely the α angle can be adjusted.

[0094] In the application of this application, not only can straight-down fasteners that can directly control the downward distance of the fastener be used, but also spiral fasteners can be used to adjust and control the downward distance of the fastener, thereby improving the precision of the downward distance adjustment to a higher level.

[0095] In this application, the distance adjusting fastener 272 is a helical fastener, including a set screw. The helical fastener controls its downward distance by rotating an angle; the lead S is the downward distance of the helical fastener in one rotation of the thread; therefore, L1 = S / 360; the range of the lead S is 0.2mm to 1mm; it can also be a limit distance adjusting fastener 272 with a lead of 0.35mm, 0.5mm, or 0.7mm; when L1 is greater than or equal to S, it will rotate at least one full rotation, at which point L1 = S × n + S / 360, where n is the number of full rotations. The adjustment of the downward distance of the distance adjusting fastener 272 is achieved by converting the fine adjustment of the downward distance into the rotation angle and number of rotations of the distance adjusting fastener 272 through the lead S. This is equivalent to converting the fine adjustment of the distance into the adjustment of the rotation angle, thus amplifying the means of fine-tuning the dimensions. Therefore, the fine adjustment of the downward distance L1 can be achieved by finely adjusting the rotation angle of the limit distance adjusting fastener 272.

[0096] For example, when the lead S of the distance adjusting fastener 272 is 0.5mm, L1 = S / 360 = 0.5mm / 360 ≈ 1.39um; this means that for every degree of rotation of the distance adjusting fastener 272, the downward distance is approximately 1.39um, achieving an adjustment in the down-distance at the um level. Even with a rotation angle based on 30 degrees, the adjustment accuracy can reach 30 × 0.5mm / 360 ≈ 41.7um, which is more precise than the mm-level dimensions.

[0097] In this application, a spiral fastener is used to improve the accuracy of distance adjustment by using the distance adjustment fastener 272. This allows for finer adjustment of distance L1 in α=arctag(L1 / R1), resulting in finer adjustment of the α angle, and consequently, finer adjustment of distance L2 in L2=R2-R2×cosα. Balancing component machining accuracy and cost, this method achieves high-precision dimensional adjustment with relatively low machining accuracy, representing an efficient and low-cost solution. It enables high-precision position adjustment with low-precision component machining dimensions. In other words, the millimeter-level distance adjustment between the bottom 261 of the imaging lens holder and the adjustment guide seat 270 can be translated into a finer dimensional distance adjustment of the objective lens 610 along the optical axis.

[0098] The imaging component support device, base, and microscopic image acquisition device and adjustment method designed in this application include an imaging lens support, an imaging lens holder, and an adjustment guide seat. The bottom of the imaging lens holder is movably engaged between the imaging lens support and the adjustment guide seat. The horizontality of the upper surface of the bottom of the imaging lens holder relative to the bottom of the imaging lens support can be adjusted until the microscopic imaging component fixed on the imaging lens holder is orthogonal to the upper surface of the bottom of the imaging lens holder, thus fixing the microscopic imaging component on the imaging lens holder. The adjustment of the distance in millimeters between the bottom of the imaging lens holder and the adjustment guide seat is transformed into a more precise adjustment of the distance in the optical axis direction of the objective lens, thereby achieving a high-precision optical adjustment process.

[0099] like Figures 1 to 23 The above description is merely an embodiment of this application and does not limit the patent scope of this application. Any equivalent structural or procedural transformations made using the content of the invention specification and drawings, or direct or indirect applications in other related technical fields, are similarly included within the patent protection scope of this application.

Claims

1. An imaging component carrying device (200), characterized in that, Used as a carrier for imaging components in microscopic imaging acquisition devices; It includes an imaging lens holder (230), an imaging lens clamp (260), and an adjustment guide seat (270); The adjusting guide seat (270) is fixedly connected to the imaging lens bracket (230); The imaging lens holder (260) includes an imaging lens holder bottom (261) and a lens holding part (262); the imaging lens holder lens through hole (266) passes through the imaging lens holder bottom (261) and the lens holding part (262); the imaging lens bracket (230) is provided with a bracket lens through hole (231); The adjusting guide seat (270) is provided with a guide seat through hole (279); The lens clamping part (262) passes through the guide seat through hole (279) on the adjustment guide seat (270), so that the bottom (261) of the imaging lens clamping seat is movably engaged between the imaging lens bracket (230) and the adjustment guide seat (270); When the bottom (261) of the imaging lens holder is engaged between the imaging lens bracket and the adjustment guide seat, the positions of the lens through hole (231) of the bracket and the lens through hole (266) of the imaging lens holder correspond; The imaging lens holder (230) includes an imaging lens holder base (232); The support lens through hole (231) is set on the bottom (232) of the imaging lens support; At least three first elastic support members (233) are provided on the bottom (232) of the imaging lens holder. The first elastic support members (233) are evenly distributed around the lens through hole (231) of the holder. The elastic support surface or elastic support point of the first elastic support member (233) faces upward to support the bottom (261) of the imaging lens holder.

2. The imaging component carrier device (200) according to claim 1, characterized in that, The adjusting guide seat (270) is provided with at least three limit position adjusting holes (271), and a limit distance adjusting fastener (272) is provided in the limit position adjusting hole (271); The positions of each limit position adjustment hole (271) correspond to the positions of each first elastic support (233).

3. The imaging component carrier device (200) according to claim 2, characterized in that, The limit position adjustment hole (271) is a limit position adjustment through hole; The limiting distance adjusting fastener (272) passes through the limiting position adjusting through hole and contacts the bottom (261) of the imaging lens holder.

4. The imaging component carrier device (200) according to claim 2 or 3, characterized in that, The limiting distance adjusting fastener (272) is a helical fastener. The downward distance of the helical fastener is controlled by the rotation angle. The lead S is the downward distance of the helical fastener when the thread rotates one revolution. The lead S of the helical fastener is in the range of 0.2mm to 1mm.

5. The imaging component carrying device according to claim 2 or 3, characterized in that, On the adjusting guide seat (270), two position fixing through holes A1 (275) are symmetrically arranged on both sides of each limiting position adjusting hole (271); Correspondingly, two position fixing through holes B1 (265) are also provided on the bottom (261) of the imaging lens holder; Each fixing fastener passes through the corresponding fixing through hole A1 (275) and fixing through hole B1 (265) in sequence to fix the relative position between the adjusting guide seat (270) and the bottom (261) of the imaging lens holder.

6. The imaging component carrying device according to claim 1, characterized in that, When the bottom (261) of the imaging lens holder is pressed against the first elastic support (233), the downward distance of the first elastic support (233) ranges from 0.3 mm to 2 mm.

7. The imaging component carrying device according to claim 1, characterized in that, At least three second elastic supports (276) are provided on the adjustment guide seat (270) for radial support of the bottom (261) of the imaging lens holder; the second elastic supports (276) are evenly distributed around the geometric center of the adjustment guide seat (270) and are radially arranged around the lens through hole (266) of the imaging lens holder; the elastic support surface or elastic support point of the second elastic support (276) faces the radial center of the lens through hole (266) of the imaging lens holder and is used to contact the side wall of the bottom (261) of the imaging lens holder.

8. The imaging component carrier device (200) according to claim 1, characterized in that, It also includes a motor bracket (210) and a movable plate (220); The motor bracket (210) is used to fix the motor that drives the imaging assembly to move; The movable plate (220) is used to connect to the drive force output device of the motor; The imaging lens holder (230) and the movable plate (220) are fixedly connected, and the imaging lens holder (230) obtains the power to move by means of the movable plate (220).

9. The imaging component carrier device (200) according to claim 8, characterized in that, It also includes a motor body (280) and a drive force output device (290); The motor body (280) is fixed on the motor bracket (210); the rotating shaft of the motor body (280) is connected to the driving force output device (290); the driving force output device (290) is connected to the movable plate (220) and drives the movable plate (220) to move.

10. The imaging component carrier device (200) according to claim 1, characterized in that, The imaging lens bracket (230) also includes an imaging lens bracket vertical surface (235) and two imaging lens bracket support half-side surfaces (236); the imaging lens bracket (230) is fixedly connected to the imaging lens bracket vertical surface (235) and the movable plate (220) so that the imaging lens bracket (230) can move with the movable plate (220); the bottom (232) of the imaging lens bracket and the imaging lens bracket vertical surface (235) are fixedly connected to each other through the two imaging lens bracket support half-side surfaces (236).

11. An imaging component support base for supporting a microscopic imaging acquisition device, characterized in that, Includes the imaging component carrier device (200) as described in any one of claims 1 to 9; It also includes a carrier substrate (120), a first carrier support (110), a second carrier support (130), and an imaging target clamping assembly (300); The first support bracket (110) and the second support bracket (130) are respectively disposed on the support base plate (120); The first support bracket (110) is used to support the imaging component support device (200); The second support bracket (130) is used to support the imaging target clamping assembly (300), so that the imaging target clamping assembly (300) is positioned below the imaging assembly support device (200); The first support bracket (110) includes a longitudinal support column (111) and a transverse support arm (112), with one end of the longitudinal support column (111) and the transverse support arm (112) fixedly connected. A horizontal support arm fixing through hole (1123) is provided on the outward extension part (1122) of the horizontal support arm, and the imaging component carrier (200) is fixed on the horizontal support arm (112) by means of the horizontal support arm fixing through hole (1123); The second support bracket (130) is provided with a second support through hole (133), and the center position of the horizontal support arm fixing through hole (1123) and the center position of the second support through hole (133) are fixed relative to each other.

12. The imaging component support platform according to claim 11, characterized in that, The outward extension of the lateral support arm (1122) and the longitudinal support column (111) are reinforced by a reinforcing bracket (113).

13. The imaging component support platform according to claim 11, characterized in that, The bottom of the support base plate (120) is provided with multiple shock-absorbing damping feet (125).

14. The imaging component support platform according to claim 11, characterized in that, The imaging target clamping assembly (300) includes an XY axis moving platform assembly (310) and a reagent card clamping base assembly (320); The reagent card holder assembly (320) is fixed to the top of the XY axis moving platform assembly (310); The XY axis moving platform assembly (310) is fixed to the top of the second support bracket (130).

15. The imaging component support platform according to claim 14, characterized in that, The reagent card holder assembly (320) includes a reagent card holder body (321) and a reagent card holding part (322); The reagent card holder (322) is used to hold externally inserted reagent cards; The bottom surface of the reagent card holder body (321) is parallel to the bottom surface of the reagent card holder part (322).

16. The imaging component support platform according to claim 14, characterized in that, The second support bracket (130) is provided with multiple third elastic support members for adjusting the levelness of the bottom surface of the XY axis moving platform assembly (310).

17. The imaging component support platform according to claim 14, characterized in that, Multiple fourth elastic supports are provided on the top surface of the XY axis moving platform assembly (310) for adjusting the levelness of the bottom surface of the reagent card holder assembly (320).

18. The imaging component support platform according to claim 11, characterized in that, It also includes a light source assembly (500); On the second support bracket (130), a light source fixing frustum (136) is provided with the second support through hole (133) as the center; the light source assembly (500) is fixed on the light source fixing frustum (136); so that the light emitted from the light source in the light source assembly (500) and the imaging lens in the imaging assembly support device (200) are on the same imaging optical axis.

19. A microscopic image acquisition device, characterized in that, Including the imaging component carrier device (200) as described in any one of claims 1 to 10, Or it may include the imaging component support base as described in any one of claims 11 to 18; It also includes a microscopic imaging assembly (600), which is fixed on an imaging lens holder (260).

20. A method for adjusting a microscope image acquisition device, characterized in that, Based on the imaging component carrier device (200) according to claim 3; And a microscopic imaging assembly (600) fixed on an imaging lens holder (260); The microscopic imaging assembly (600) includes an objective lens (610); The objective lens (610) passes through the imaging lens holder (260), and the objective lens (610) is clamped and fixed by the imaging lens holder (260). One end of the objective lens (610) is located below the plane of the bottom (261) of the imaging lens holder; the target imaging optical axis passes through the objective lens (610). Includes the following steps: Step A: Obtain the offset direction between the lens through hole (266) of the imaging lens holder and the target imaging optical axis. If the offset angle between the plane where the lens through hole (266) of the imaging lens holder is located and the target imaging optical axis is greater than the set target value, then proceed to step B. Step B: Select the limiting distance adjusting fastener (272) in the limiting position adjusting hole (271) closest to the end of the objective lens (610), adjust the depth of the limiting distance adjusting fastener (272) as it passes downward, and adjust the force of the bottom (261) of the imaging lens holder pressing the corresponding first elastic support (233). Step C: Check again the offset angle between the plane of the imaging lens holder lens through hole (266) and the target imaging optical axis. If the offset angle is greater than the set target value, return to step B; until the offset angle between the plane of the imaging lens holder lens through hole (266) and the target imaging optical axis is less than or equal to the set target value.

21. The adjustment method of the microscopic image acquisition device according to claim 20, characterized in that, It also includes step D: when the offset angle between the plane of the imaging lens holder lens through hole (266) and the target imaging optical axis is less than or equal to the set target value, the bottom (261) of the imaging lens holder and the adjustment guide seat (270) are fixedly connected.

Images