A pesticide spreading agent for green orange trees and its preparation method

Abstract

The present invention discloses a pesticide spreading agent for green orange trees and a preparation method thereof. The active ingredients of the pesticide spreading agent for green orange trees disclosed by the present invention are polyoxyethylene sorbitan trioleate and sodium dodecylbenzenesulfonate. The spreading agent for green oranges of the present invention can reduce the contact angle between the liquid droplets of the liquid medicine and the green orange leaf surface, change the surface tension, achieve the maximum coverage, effectively improve the adhesion, wetting and spreading ability of the droplets on the green orange leaves, enable the pesticide to be effectively deposited and covered on the green orange tree leaves, reduce the loss of the liquid medicine, reduce the drift of the droplets, improve the utilization rate of the pesticide, and also has the characteristics of low cost and environmental friendliness. It can be used for the spraying operations of various spraying pesticides on green orange trees, including insecticides, fungicides, herbicides, plant growth regulators, foliar fertilizers, etc.

Description

Technical Field

[0001] The present invention relates to a pesticide spreading agent for green orange trees and a preparation method thereof in the field of plant protection. Background Art

[0002] China is the world's largest citrus producer. The citrus industry is a pillar industry in southern China and one of the main fruit tree industries in Hainan. Hainan citrus fruits represented by Qiongzhong green oranges, Chengmai Fucheng oranges, green tangerines, green grapefruits, and perfume lemons are distinctively featured and highly competitive in the market, enjoying a high reputation in domestic and international markets. Qiongzhong green orange is the first geographical indication product in Hainan Province. In recent years, the citrus industry in Hainan has developed rapidly. By the end of 2019, the planting area had reached 182,700 mu, and the planting area is still expanding rapidly. Hainan is located in the tropical region, and the high-temperature and high-humidity climate environment is very conducive to the breeding and spread of pests, diseases, and weeds. The rich biological resources can continuously provide hosts and breeding places for harmful organisms. Due to continuous agricultural production, the annual average pesticide usage is correspondingly higher than that in temperate regions.

[0003] Research at home and abroad shows that spray functional additives are the key technology to achieve pesticide reduction and efficiency increase. Japan has achieved pesticide reduction and efficiency increase by developing and using a series of green pesticide spreading agents for fruit trees. As an automated and efficient modern pesticide application technology and method, plant protection drones can meet the requirement of labor shortage in orchards. However, the pesticide concentration in drone spray operations is usually dozens to hundreds of times higher than that in ground conventional equipment spray operations. During and for some time after the spray operation, it is extremely easy to cause phytotoxicity, and the pesticide spray drift causes varying degrees of air pollution and damage to human and animal health in the surrounding areas. Therefore, the application of pesticide spreading agents is a key way to achieve pesticide reduction and efficiency increase in China. Summary of the Invention

[0004] The technical problem to be solved by the present invention is how to significantly reduce the contact angle and surface tension between the liquid medicine or foliar fertilizer droplets and the green orange leaf surface, so as to improve the drug effect or fertilizer effect, reduce the usage of pesticides and foliar fertilizers, and reduce environmental pollution.

[0005] To solve the above technical problems, the present invention provides a spreading agent for green oranges, and its active ingredient (effective ingredient) is polyoxyethylene sorbitan trioleate and sodium dodecylbenzenesulfonate.

[0006] In the above spreading agent for green oranges, those skilled in the art can determine the ratio of polyoxyethylene sorbitan trioleate and sodium dodecylbenzenesulfonate according to the effect of improving the drug effect and fertilizer effect of the spreading agent for green oranges. For example, the mass ratio of polyoxyethylene sorbitan trioleate and sodium dodecylbenzenesulfonate is 3:7.

[0007] The above spreading agent for green oranges may also contain auxiliary materials, and the auxiliary materials may be humectants, wetting agents, defoamers, preservatives, and / or water.

[0008] In actual production, if the production volume of the spreading agent for green oranges is small, defoaming agent may not be added; if the production volume of the spreading agent for green oranges is large, defoaming agent can be used.

[0009] In actual production, if the storage time of the spreading agent for green oranges is short, preservative may not be used.

[0010] The spreading agent for green oranges specifically described above may be composed of polyoxyethylene sorbitan trioleate, sodium dodecylbenzenesulfonate and dimethyl silicone oil.

[0011] In the spreading agent for green oranges, the mass ratio of polyoxyethylene sorbitan trioleate, sodium dodecylbenzenesulfonate and dimethyl silicone oil may be 75:175:1.

[0012] The spreading agent for green oranges described above can be prepared according to the preparation method of the spreading agent for green oranges.

[0013] The preparation method of the spreading agent for green oranges includes: mixing polyoxyethylene sorbitan trioleate, dimethyl silicone oil with two products of Shanghai Kao Corporation with the catalog numbers NEOPELEX G-30 and NEOPELEX G-20 respectively for homogenization to obtain the spreading agent for green oranges, wherein the mass ratio of polyoxyethylene sorbitan trioleate, dimethyl silicone oil to the two products of Shanghai Kao Corporation with the catalog numbers NEOPELEX G-30 and NEOPELEX G-20 respectively is 75:1:150:25.

[0014] In the above preparation method, the homogenization can be carried out by colloid mill homogenization; the gap between the stator and the rotor used in the colloid mill homogenization is 2 microns, the rotor linear velocity is 1000 - 1500 revolutions per minute, 1500 - 2000 revolutions per minute or 2000 - 2500 revolutions per minute, the homogenization time is 1 - 2 minutes, and the homogenized liquid is allowed to stand at 35°C or below for 1 - 2 hours to obtain the spreading agent for green oranges.

[0015] The application of polyoxyethylene sorbitan trioleate and sodium dodecylbenzenesulfonate in the preparation of the spreading agent for green oranges also belongs to the protection scope of the present invention.

[0016] The application of polyoxyethylene sorbitan trioleate and sodium dodecylbenzenesulfonate in improving the efficacy of the medicament on green oranges and / or improving the fertilizer efficiency of the foliar fertilizer on green oranges, wherein the medicament is a medicament that can be applied to green oranges by spraying, also belongs to the protection scope of the present invention.

[0017] The application of polyoxyethylene sorbitan trioleate and sodium dodecylbenzenesulfonate in preventing and controlling green orange pests and / or diseases also belongs to the protection scope of the present invention.

[0018] The present invention also provides a method for improving the efficacy of pesticides on green oranges and / or the fertilizer efficiency of foliar fertilizers on green oranges. The method includes formulating the pesticide and / or foliar fertilizer into a spray solution, adding a spreading agent for green oranges to the spray solution to obtain a spray solution containing the spreading agent for green oranges, and then applying the spray solution containing the spreading agent for green oranges to green oranges by spraying to improve the efficacy of the pesticide on green oranges and / or the fertilizer efficiency of the foliar fertilizer on green oranges; the pesticide is a pesticide that can be applied to green oranges by spraying.

[0019] The mass content of the spreading agent for green oranges in the spray solution containing the spreading agent for green oranges can be 0.025 - 2% (such as 0.025%, 0.05%, 0.1%, 0.25%, 0.5%, 1%, 2%).

[0020] In the above method, the pesticide can be a bactericide, an insecticide, and / or a plant growth regulator.

[0021] In an embodiment of the present invention, the pesticide is 21% thiamethoxam suspension concentrate (produced by Liuzhou Huinong Chemical Co., Ltd.).

[0022] The spreading agent for green oranges of the present invention is also a spray adjuvant and is a non-ionic and anionic mixed adjuvant.

[0023] The spreading agent for green oranges of the present invention can reduce the contact angle between the liquid droplets of the liquid medicine and the green orange leaf surface, change the surface tension, achieve the maximum coverage, effectively improve the adhesion, wetting, and spreading ability of the droplets on the green orange leaves, enable the pesticide to be effectively deposited and covered on the green orange tree leaves, reduce the loss of the liquid medicine, reduce the drift of the droplets, and improve the pesticide utilization rate; the spreading agent for green oranges of the present invention also has the characteristics of low cost and environmental friendliness. The spreading agent for green oranges of the present invention is applicable to various spray pesticides for green orange trees, including the spray operations of insecticides, bactericides, herbicides, plant growth regulators, foliar fertilizers, etc. The spreading agent for green oranges of the present invention can be used in both aerial plant protection operations and conventional ground spray pesticides, and has the characteristics of safety, environmental protection, excellent performance, and simple manufacturing process, and has a good application prospect.

[0024] The present invention is a special spreading agent developed with the support of scientific research projects such as the special project of the basic scientific research business expenses of the central-level public welfare scientific research institute "Research on the synergistic mechanism of flying prevention and control of Diaphorina citri based on pesticide spreading agents" (project number: 1630042021006) and the key research and development plan project of Hainan Province "Research and application of green pesticide spreading agents for citrus (green orange) trees" (project number: ZDYF2022XDNY201), and can be added to the diluted pesticide for spray application.

[0025] The present invention will be further described in detail below in conjunction with specific embodiments. The provided embodiments are only for clarifying the present invention and not for limiting the scope of the present invention. The following provided embodiments can be used as a guide for those of ordinary skill in the art to make further improvements, and do not constitute any limitation to the present invention in any way. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] Figure 1 The state after 10 minutes when the diluent of spreading agent 1 was dropped on the front and back of the green orange leaves for comparison.

[0027] Figure 2 The state after 10 minutes when the diluent of the spreading agent for green orange was dropped on the front and back of the green orange leaves.

[0028] Figure 3 The state after 10 minutes when the diluent of the spreading agent for green orange was dropped on the front and back of the green orange leaves.

[0029] Figure 4 The green orange new leaves damaged by Diaphorina citri.

[0030] Figure 5 The state after 10 minutes when the drug was diluted 3500 times with the spreading agent for green orange and dropped on the front and back of the green orange leaves.

[0031] Figure 6 The state after 10 minutes when the drug was diluted 140 times with the spreading agent for green orange and dropped on the front and back of the green orange leaves. DETAILED DESCRIPTION OF THE EMBODIMENTS

[0032] The experimental methods in the following embodiments are all conventional methods unless otherwise specified, and are carried out according to the techniques or conditions described in the literature in this field or according to the product instructions. The materials, reagents, instruments, etc. used in the following embodiments can be obtained from commercial channels unless otherwise specified. In the following embodiments, quantitative tests are all set with three repeated experiments, and the results are averaged.

[0033] The polyoxyethylene sorbitan trioleate (Tween 85 or polyoxyethylene sorbitan trioleate) in the following embodiments is a product of Kao (Shanghai) Trading Co., Ltd., and its catalog number is RHEODOL TW-O320V. Acid Value 1.50 mg KOH / g, Saponification Value 86.1 mg KOH / g, Hydroxyl Value 46.4 mg KOH / g, water content 4.9%, Color (Gardner) Gardner colorimetric value 4, content label: 100%, CAS number: 9005-70-3.

[0034] Sodium dodecylbenzenesulfonate 20 in the following examples is a product of Kao Chemical Co., Ltd., Shanghai, with a catalog number of NEOPELEX G-20, Chemical Abstracts Service Registry Number: 69669-44-9, and an active matter content of 20.0%. Appearance passed, Color (klett) chromaticity 5, Petroleum Ether-Soluble Matter 0.3%, Loss on Drying 78.8%, pH 7.4, sodium sulfate 0.4%.

[0035] Sodium dodecylbenzenesulfonate 30 in the following examples is a product of Kao Chemical Co., Ltd., Shanghai, with a catalog number of NEOPELEX G-30, Chemical Abstracts Service Registry Number: 69669-44-9, and an active matter content of 30.4%. Color (klett) chromaticity 5, Unsulfated Matter 0.4%, Loss on Drying 68.3%, pH 7.5, sodium sulfate 0.6%.

[0036] The dimethyl silicone oil in the following examples is a product of Shanghai Macklin Biochemical Co., Ltd., CAS: 63148-62-9, with a viscosity of 100 ± 8 Pa·s.

[0037] Example 1 Preparation of spreading agent for green oranges

[0038] The inventor of the present invention screened out polyoxyethylene sorbitan trioleate as the active ingredient of the spreading agent for green oranges from a large number of existing fatty acid esters, and then formulated it with auxiliary materials (such as moisturizers, wetting agents, defoamers, preservatives and water) to prepare the spreading agent for green oranges.

[0039] 1. Preparation of spreading agent for green oranges

[0040] The spreading agent for green oranges is composed of polyoxyethylene sorbitan trioleate, sodium dodecylbenzenesulfonate 30, sodium dodecylbenzenesulfonate 20 and dimethyl silicone oil. Among them, the mass ratio of polyoxyethylene sorbitan trioleate, sodium dodecylbenzenesulfonate 30, sodium dodecylbenzenesulfonate 20, and dimethyl silicone oil is 75:150:25:1.

[0041] The preparation method of the spreading agent for green oranges is as follows: Mix polyoxyethylene sorbitan trioleate, sodium dodecylbenzenesulfonate 30, sodium dodecylbenzenesulfonate 20, and dimethyl silicone oil evenly to obtain a mixed solution; Inject the mixed solution into a colloid mill and homogenize it under the following conditions to obtain a homogenized solution: The gap between the stator and rotor of the colloid mill is 2 microns, the rotor linear velocity is 1500 revolutions per minute, and the homogenization time is 2 minutes. Let the homogenized solution stand at 35°C for 1 - 2 hours to obtain the spreading agent for green oranges.

[0042] 2. Preparation of control spreading agents

[0043] Prepare control spreading agent 1 and control spreading agent 2 according to the method in Step 1:

[0044] Control spreading agent 1 is composed of polyoxyethylene sorbitan trioleate, dimethyl silicone oil and water. Among them, the mass ratio of polyoxyethylene sorbitan trioleate, dimethyl silicone oil and water is 150:1:100.

[0045] The preparation method of control spreading agent 1 is as follows: Mix polyoxyethylene sorbitan trioleate, dimethyl silicone oil and water evenly to obtain a mixed solution; Inject the mixed solution into a colloid mill and carry out homogenization under the following conditions to obtain a homogenized solution: The gap between the stator and the rotor of the colloid mill is 2 microns, the rotor linear velocity is 1500 revolutions per minute, and the homogenization time is 2 minutes. Let the homogenized solution stand at 35°C for 1 - 2 hours to obtain control spreading agent 1.

[0046] Control spreading agent 2 is composed of sodium dodecylbenzenesulfonate 20 and dimethyl silicone oil, and the mass ratio of sodium dodecylbenzenesulfonate 20 and dimethyl silicone oil is 250:1.

[0047] The preparation method of control spreading agent 2 is as follows: Mix sodium dodecylbenzenesulfonate 20 and dimethyl silicone oil evenly to obtain a mixed solution; Inject the mixed solution into a colloid mill and carry out homogenization under the following conditions to obtain a homogenized solution: The gap between the stator and the rotor of the colloid mill is 2 microns, the rotor linear velocity is 1500 revolutions per minute, and the homogenization time is 2 minutes. Let the homogenized solution stand at 35°C for 1 - 2 hours to obtain control spreading agent 2.

[0048] Example 2. Detection of the spreading property of the spreading agent in Example 1

[0049] This example evaluates the spreading property of the spreading agent in Example 1. The spreading, adhesion and wetting functions of the spreading agent are evaluated by contact angle, surface tension and viscosity.

[0050] 1. Detection of contact angle

[0051] The preparation method of each group of test liquid to be measured is as follows:

[0052] Spreading agent diluted 100 times group (denoted as A5 G-100 group): Dilute the green orange spreading agent in Example 1 100 times with distilled water to obtain a diluted solution of the green orange spreading agent. The mass content of the green orange spreading agent in Example 1 in the diluted solution of the green orange spreading agent is 1%;

[0053] Spreading agent diluted 200 times group (denoted as A5 G-200 group): Dilute the green orange spreading agent in Example 1 200 times with distilled water to obtain a diluted solution of the green orange spreading agent. The mass content of the green orange spreading agent in Example 1 in the diluted solution of the green orange spreading agent is 0.5%;

[0054] Spreader dilution 400-fold group (denoted as A5 G-400 group): The green oranges of Example 1 were diluted 400-fold with distilled water using the spreader to obtain a spreader dilution of green oranges. The mass content of the spreader for the green oranges of Example 1 in the spreader dilution of green oranges was 0.25%;

[0055] Spreader dilution 1000-fold group (denoted as A5 G-1000 group): The green oranges of Example 1 were diluted 1000-fold with distilled water using the spreader to obtain a spreader dilution of green oranges. The mass content of the spreader for the green oranges of Example 1 in the spreader dilution of green oranges was 0.1%;

[0056] Spreader dilution 2000-fold group (denoted as A5 G-2000 group): The green oranges of Example 1 were diluted 2000-fold with distilled water using the spreader to obtain a spreader dilution of green oranges. The mass content of the spreader for the green oranges of Example 1 in the spreader dilution of green oranges was 0.05%;

[0057] Spreader dilution 4000-fold group (denoted as A5 G-4000 group): The green oranges of Example 1 were diluted 4000-fold with distilled water using the spreader to obtain a spreader dilution of green oranges. The mass content of the spreader for the green oranges of Example 1 in the spreader dilution of green oranges was 0.025%;

[0058] Control spreader 1 dilution 100-fold group (denoted as A5-100 group): The control spreader 1 of Example 1 was diluted 100-fold with distilled water to obtain a control spreader 1 dilution. The mass content of the control spreader 1 of Example 1 in the control spreader 1 dilution was 1%;

[0059] Control spreader 1 dilution 200-fold group (denoted as A5-200 group): The control spreader 1 of Example 1 was diluted 200-fold with distilled water to obtain a control spreader 1 dilution. The mass content of the control spreader 1 of Example 1 in the control spreader 1 dilution was 0.5%;

[0060] Control spreader 1 dilution 400-fold group (denoted as A5-400 group): The control spreader 1 of Example 1 was diluted 400-fold with distilled water to obtain a control spreader 1 dilution. The mass content of the control spreader 1 of Example 1 in the control spreader 1 dilution was 0.25%;

[0061] Control spreader 1 dilution 1000-fold group (denoted as A5-1000 group): The control spreader 1 of Example 1 was diluted 1000-fold with distilled water to obtain a control spreader 1 dilution. The mass content of the control spreader 1 of Example 1 in the control spreader 1 dilution was 0.1%;

[0062] Control spreading agent 1 diluted 2000-fold group (denoted as A5-2000 group): Dilute the control spreading agent 1 in Example 1 2000-fold with distilled water to obtain a diluted solution of the control spreading agent 1. The mass content of the control spreading agent 1 in Example 1 in the diluted solution of the control spreading agent 1 is 0.05%.

[0063] Control spreading agent 1 diluted 4000-fold group (denoted as A5-4000 group): Dilute the control spreading agent 1 in Example 1 4000-fold with distilled water to obtain a diluted solution of the control spreading agent 1. The mass content of the control spreading agent 1 in Example 1 in the diluted solution of the control spreading agent 1 is 0.025%.

[0064] Control spreading agent 2 diluted 100-fold group (denoted as G-100 group): Dilute the control spreading agent 2 in Example 1 100-fold with distilled water to obtain a diluted solution of the control spreading agent 2. The mass content of the control spreading agent 2 in Example 1 in the diluted solution of the control spreading agent 2 is 1%.

[0065] Control spreading agent 2 diluted 200-fold group (denoted as G-200 group): Dilute the control spreading agent 2 in Example 1 200-fold with distilled water to obtain a diluted solution of the control spreading agent 2. The mass content of the control spreading agent 2 in Example 1 in the diluted solution of the control spreading agent 2 is 0.5%.

[0066] Control spreading agent 2 diluted 400-fold group (denoted as G-400 group): Dilute the control spreading agent 2 in Example 1 400-fold with distilled water to obtain a diluted solution of the control spreading agent 2. The mass content of the control spreading agent 2 in Example 1 in the diluted solution of the control spreading agent 2 is 0.25%.

[0067] Control spreading agent 2 diluted 1000-fold group (denoted as G-1000 group): Dilute the control spreading agent 2 in Example 1 1000-fold with distilled water to obtain a diluted solution of the control spreading agent 2. The mass content of the control spreading agent 2 in Example 1 in the diluted solution of the control spreading agent 2 is 0.1%.

[0068] Control spreading agent 2 diluted 2000-fold group (denoted as G-2000 group): Dilute the control spreading agent 2 in Example 1 2000-fold with distilled water to obtain a diluted solution of the control spreading agent 2. The mass content of the control spreading agent 2 in Example 1 in the diluted solution of the control spreading agent 2 is 0.05%.

[0069] Control spreading agent 2 diluted 4000-fold group (denoted as G-4000 group): Dilute the control spreading agent 2 in Example 1 4000-fold with distilled water to obtain a diluted solution of the control spreading agent 2. The mass content of the control spreading agent 2 in Example 1 in the diluted solution of the control spreading agent 2 is 0.025%.

[0070] The contact angle was measured using a contact angle measuring instrument according to the following method: Representative mature leaves with dark green color and various sizes were collected, randomly grouped, and placed on glass slides, with two leaves on each glass slide, one with the front side up and the other with the back side up. The glass slides with the leaves were placed on the contact angle measuring instrument (model JC2000D1, produced by Shanghai Zhongchen Digital Technology Equipment Co., Ltd.). The liquid medicine to be tested was taken with a syringe and placed on the contact angle measuring instrument. 2 μl of the liquid medicine was respectively dropped on the front and back sides of the leaves. The contact angles of the liquid medicine on the leaves at different times (1 min, 5 min, 10 min, 15 min) were measured by the contact angle measuring instrument. For the front and back sides of the same amount of leaves treated with each liquid medicine to be tested, distilled water was used instead of the liquid medicine to be tested as a control. Each liquid medicine was measured 3 times, and the average value was calculated. Except for the different liquids sprayed on the leaves, other operations for each treatment were exactly the same.

[0071] Table 1. Results of contact angle measurement of 100-fold aqueous dilution of spreading agent on green orange leaves

[0072]

[0073] Note: Different lowercase letters in the same column of data indicate significant differences at the P<0.05 level by LSD method test, and those with the same lowercase letters have no significant differences.

[0074] Table 2. Results of contact angle measurement of 200-fold aqueous dilution of spreading agent on green orange leaves

[0075]

[0076] Note: Different lowercase letters in the same column of data indicate significant differences at the P<0.05 level by LSD method test, and those with the same lowercase letters have no significant differences.

[0077] Table 3. Results of contact angle measurement of 400-fold aqueous dilution of spreading agent on green orange leaves

[0078]

[0079] Note: Different lowercase letters in the same column of data indicate significant differences at the P<0.05 level by LSD method test, and those with the same lowercase letters have no significant differences.

[0080] Table 4. Results of contact angle measurement of 1000-fold aqueous dilution of spreading agent on green orange leaves

[0081]

[0082] Note: Different lowercase letters in the same column of data indicate significant differences at the P<0.05 level by LSD method test, and those with the same lowercase letters have no significant differences.

[0083] Table 5. Results of contact angle measurement on leaves of green oranges with 2000-fold aqueous dilution of spreading agent

[0084]

[0085] Note: Different lowercase letters in the same column of data indicate significant differences at the P<0.05 level by LSD method, and those with the same lowercase letters have no significant differences.

[0086] Table 6. Results of contact angle measurement on leaves of green oranges with 4000-fold aqueous dilution of spreading agent

[0087]

[0088] Note: Different lowercase letters in the same column of data indicate significant differences at the P<0.05 level by LSD method, and those with the same lowercase letters have no significant differences.

[0089] The results are shown in Table 1-6. Figure 1-3 . The results show that the contact angles on both the front and back sides of the leaves of green oranges with the spreading agent, control spreading agents 1 and 2 are significantly lower than those of the control group (water); when diluted 200 - 2000 times, whether on the front or back side of the leaves, the contact angles of the spreading agent for green oranges at 10 - 15 minutes are significantly lower than those of control spreading agent 1 and control spreading agent 2; when diluted 100 times, the contact angles of the spreading agent for green oranges on the back side of the leaves are significantly lower than those of the two control spreading agents, and the contact angles at short contact times (1 - 5 minutes) on the front side of the leaves are significantly lower than those of the two control spreading agents; when diluted 4000 times, the contact angles of the spreading agent for green oranges at 15 minutes are significantly lower than those of the two control spreading agents. It shows that the spreading agent for green oranges has good spreading properties and better effects at high dilution multiples.

[0090] 2. Detection of surface tension and viscosity

[0091] The spreading agent for green oranges in Example 1, control spreading agent 1 and control spreading agent 2 were each diluted 2, 20, 200 and 2000 times with distilled water to obtain dilution solutions of different dilution multiples of each spreading agent as the test medicaments.

[0092] Using a fully automatic tensiometer (model JK99F, produced by Shanghai Zhongchen Digital Technology Equipment Co., Ltd.), the platinum plate method was used to detect the surface tension of water, each spreading agent and their dilution solutions of different dilution multiples. Before the experiment, the fully automatic tensiometer needs to be calibrated with weights, and the platinum plate needs to be cleaned and burned before measuring each sample. The test temperature is 30±0.3°C. Each liquid was measured 3 times repeatedly, and the average value was calculated. The results are shown in Table 2.

[0093] The viscosity of the diluents of water and various spreading agents with different dilution multiples was detected using a DNJ-5S digital display rotational viscometer (Shanghai Hengping Instrument and Meter Factory). The temperature of the sample to be measured was controlled using a constant temperature water bath. An appropriate rotor and rotation speed were selected to keep the torque reading value between 25% and 75%, and the viscosity value was recorded. The measurement was repeated 3 times. The difference between the results should be less than 5%. Each liquid medicine was measured 3 times and the average value was calculated. The results are shown in Table 7-8.

[0094] Table 7. Determination of the surface tension of the diluents of the spreading agent for green oranges with water

[0095]

[0096]

[0097] Note: Different lowercase letters marked for the data in the same column indicate significant differences at the P<0.05 level by LSD method test, and those marked with the same lowercase letters have no significant differences.

[0098] Table 8. Determination of the viscosity of the diluents of the spreading agent for green oranges with water

[0099]

[0100] Note: The viscosity value > 2×10 6 mPa·s exceeds the maximum detection limit of the instrument; " / " indicates that it exceeds the minimum detection limit of the instrument and is close to 0.

[0101] The test data in Table 7 show that: compared with the viscosity value of the control spreading agent 1, after no dilution or dilution with water by 2 times, it exceeded the maximum detection limit. Therefore, too high viscosity is not conducive to filling and use; the viscosity values of the spreading agents for green oranges were 588.77 and 1845.33 mN / M respectively after no dilution or dilution with water by 2 times; the viscosity value of the control spreading agent 2 after dilution with water by 2 times was 424.80.

[0102] The test data in Table 8 show that: after the three spreading agents were diluted with water by 20 times, 200 times, and 2000 times, the surface tension values increased slightly accordingly. When the spreading agent for green oranges was diluted by 200 times and 2000 times, the surface tension was significantly less than that of the control spreading agent 1 and the control spreading agent 2, indicating that the spreading agent for green oranges in Example 1 is the best spreading agent for green oranges and has a good spreading effect.

[0103] Example 3. Influence of the spreading agent in Example 1 on the spreading property of pesticides

[0104] First, dilute the green oranges in Example 1 with a spreading agent, Control Spreading Agent 1, and Control Spreading Agent 2 by adding water 100 times, 200 times, 400 times, 1000 times, 2000 times, and 4000 times respectively, to obtain the diluted solutions of the spreading agent for green oranges, Control Spreading Agent 1, and Control Spreading Agent 2 diluted 100 times, 200 times, 400 times, 1000 times, 2000 times, and 4000 times.

[0105] Dilute the 21% thiamethoxam suspension concentrate (produced by Liuzhou Huinong Chemical Co., Ltd., pesticide registration number: PD20141845, with the control target being Diaphorina citri) 140 times with each spreading agent diluted solution to obtain the drug diluted solutions of the spreading agent for green oranges - 100, the spreading agent for green oranges - 200, the spreading agent for green oranges - 400, the spreading agent for green oranges - 1000, the spreading agent for green oranges - 2000, the spreading agent for green oranges - 4000, the spreading agent 1 - 100, the spreading agent 1 - 200, the spreading agent 1 - 400, the spreading agent 1 - 1000, the spreading agent 1 - 2000, the spreading agent 1 - 4000, the spreading agent 2 - 100, the spreading agent 2 - 200, the spreading agent 2 - 400, the spreading agent 2 - 1000, the spreading agent 2 - 2000, and the spreading agent 2 - 4000, each diluted 140 times. Use each drug diluted solution as the test liquid to detect its contact angle. Dilute the 21% thiamethoxam suspension concentrate 140 times with distilled water and use pure distilled water as the control.

[0106] Dilute the 21% thiamethoxam suspension concentrate 3500 times with each spreading agent diluted solution to obtain the drug diluted solutions of the spreading agent for green oranges - 100, the spreading agent for green oranges - 200, the spreading agent for green oranges - 400, the spreading agent for green oranges - 1000, the spreading agent for green oranges - 2000, the spreading agent for green oranges - 4000, the spreading agent 1 - 100, the spreading agent 1 - 200, the spreading agent 1 - 400, the spreading agent 1 - 1000, the spreading agent 1 - 2000, the spreading agent 1 - 4000, the spreading agent 2 - 100, the spreading agent 2 - 200, the spreading agent 2 - 400, the spreading agent 2 - 1000, the spreading agent 2 - 2000, and the spreading agent 2 - 4000, each diluted 140 times. Use each drug diluted solution as the test liquid to detect its contact angle. Dilute the 21% thiamethoxam suspension concentrate 3500 times with distilled water and use pure distilled water as the control.

[0107] Collect fully expanded leaves with a fresh green color. The Asian citrus psyllid damages the new leaves of green oranges (see Figure 4 ). Randomly group the leaves and place them on glass slides, with two leaves on each slide, one facing up and one facing down. Place the glass slides with the leaves on a contact angle measuring instrument (model JC2000D1, produced by Shanghai Zhongchen Digital Technology Equipment Co., Ltd.). Use a syringe to take the test liquid and place it on the contact angle measuring instrument. Drop 2 μl of the liquid onto the front and back of the leaves respectively. Measure the contact angle of the liquid on the leaves at different times (1 min, 5 min, 10 min, 15 min) through the contact angle measuring instrument, with equal amounts of the front and back of the leaves treated with each test liquid. Repeat the measurement 3 times for each test liquid and calculate the average value. Except for the different liquids sprayed on the leaves, other operations are exactly the same for each treatment.

[0108] The results are shown in Table 9-14 and Figure 5 、 Figure 6 as follows.

[0109] Table 9. Contact Angle Measurement Results of 140-fold Diluted Thiamethoxam with a Spreader on Green Orange Leaves

[0110]

[0111]

[0112] Note: Different lowercase letters in the same column indicate significant differences at the P<0.05 level tested by the LSD method, and the same lowercase letters indicate no significant differences.

[0113] Table 10. Contact Angle Measurement Results of 140-fold Diluted Thiamethoxam with a Spreader on Green Orange Leaves

[0114]

[0115] Note: Different lowercase letters in the same column indicate significant differences at the P<0.05 level tested by the LSD method, and the same lowercase letters indicate no significant differences.

[0116] Table 11. Contact Angle Measurement Results of 140-fold Diluted Thiamethoxam with a Spreader on Green Orange Leaves

[0117]

[0118]

[0119] Note: Different lowercase letters in the same column indicate significant differences at the P<0.05 level tested by the LSD method, and the same lowercase letters indicate no significant differences.

[0120] Table 12. Contact Angle Measurement Results of Thiamethoxam 3500-fold Dilution Solution with Spreader on Green Oranges Leaves

[0121]

[0122] Note: Different lowercase letters marked for the data in the same column indicate significant differences at the P<0.05 level by LSD method test, and those marked with the same lowercase letters have no significant differences.

[0123] Table 13. Contact Angle Measurement Results of Thiamethoxam 3500-fold Dilution Solution with Spreader on Green Oranges Leaves

[0124]

[0125]

[0126] Note: Different lowercase letters marked for the data in the same column indicate significant differences at the P<0.05 level by LSD method test, and those marked with the same lowercase letters have no significant differences.

[0127] Table 14. Contact Angle Measurement Results of Thiamethoxam 3500-fold Dilution Solution with Spreader on Green Oranges Leaves

[0128]

[0129] Note: Different lowercase letters marked for the data in the same column indicate significant differences at the P<0.05 level by LSD method test, and those marked with the same lowercase letters have no significant differences.

[0130] The results show that: The contact angles of the drugs diluted with the spreader for green oranges, control spreader 1, and control spreader 2 are all significantly lower than those of the control group (water) and the drugs diluted with water; when the drug is diluted 3500 times after the spreader is diluted 4000 times, whether on the front or back of the leaves, the contact angles of the drugs diluted with the spreader for green oranges are significantly lower than those of control spreader 1 and control spreader 2; when the drug is diluted 3500 times after the spreader is diluted 1000 - 2000 times, at 10 - 15 minutes on the front of the leaves and 5 - 15 minutes on the back of the leaves, the contact angles of the drugs diluted with the spreader for green oranges are significantly lower than those of control spreader 1 and control spreader 2; while when the spreader is diluted 100 - 400 times, except for 5 minutes on the front of the leaves diluted 100 times, 15 minutes on the front of the leaves diluted 200 times, 15 minutes on the front of the leaves diluted 400 times, and 1 - 5 minutes on the back of the leaves, the contact angles of the drugs diluted with the spreader for green oranges are significantly lower than those of control spreader 1 and control spreader 2. It shows that the spreader for green oranges has good spreading characteristics and better effects at high dilution multiples.

[0131] 3. Influence of Spreader on the Surface Tension of Drugs

[0132] First, dilute the spreading agent for green oranges in Example 1, control spreading agent 1, and control spreading agent 2 by adding water 100 times, 200 times, and 400 times respectively to obtain the diluted solutions of the spreading agent for green oranges, control spreading agent 1, and control spreading agent 2 diluted 100 times, 200 times, and 400 times; use each diluted spreading agent solution to dilute the 21% thiamethoxam suspension 140 times to obtain the spreading agent for green oranges - 100 drug diluted solution, spreading agent for green oranges - 200 drug diluted solution, spreading agent for green oranges - 400 drug diluted solution, spreading agent 1 - 100 drug diluted solution, spreading agent 1 - 200 drug diluted solution, spreading agent 1 - 400 drug diluted solution, spreading agent 2 - 100 drug diluted solution, spreading agent 2 - 200 drug diluted solution, and spreading agent 2 - 400 drug diluted solution respectively;

[0133] First, dilute the spreading agent for green oranges in Example 1, control spreading agent 1, and control spreading agent 2 by adding water 1000 times, 2000 times, and 4000 times respectively to obtain the diluted solutions of the spreading agent for green oranges, control spreading agent 1, and control spreading agent 2 diluted 1000 times, 2000 times, and 4000 times; use each diluted spreading agent solution to dilute the 21% thiamethoxam suspension 3500 times to obtain the spreading agent for green oranges - 1000 drug diluted solution, spreading agent for green oranges - 2000 drug diluted solution, spreading agent for green oranges - 4000 drug diluted solution, spreading agent 1 - 1000 drug diluted solution, spreading agent 1 - 2000 drug diluted solution, spreading agent 1 - 4000 drug diluted solution, spreading agent 2 - 1000 drug diluted solution, spreading agent 2 - 2000 drug diluted solution, and spreading agent 2 - 4000 drug diluted solution respectively.

[0134] Use each drug diluted solution as the test liquid to detect its surface tension. Dilute the 21% thiamethoxam suspension 140 times and 3500 times with distilled water, and use pure distilled water as the control.

[0135] Measure the surface tension of each solution according to the method for measuring surface tension in Step 2 of Example 2, and the results are shown in Table 15.

[0136] Table 15. Measurement of the surface tension of the thiamethoxam diluted solution with the spreading agent for green oranges

[0137]

[0138]

[0139] Note: Data in the same column marked with different lowercase letters indicate significant differences at the P < 0.05 level by LSD method test, and those marked with the same lowercase letters have no significant differences.

[0140] The test data in Table 15 show that the surface tensions of the green oranges when the drug is diluted 1000 times, 2000 times, and 4000 times with the spreading agent are all significantly less than those of Control Spreading Agent 1 and Control Spreading Agent 2. This indicates that the spreading agent for green oranges in Example 1 is the best spreading agent for green oranges and has a very good spreading effect.

[0141] In addition, due to its too high viscosity, Control Spreading Agent 1 is not conducive to perfusion and use, and the cost of using it alone is also higher than that of the spreading agent for green oranges in Example 1; because of its strong permeability and easy to cause phytotoxicity to plants, and it is not as environmentally friendly, green and safe as the spreading agent for green oranges in Example 1, Control Spreading Agent 2 should not be used in excessive amounts; the spreading agent for green oranges in Example 1 can not only effectively improve the adhesion, wetting and spreading ability of droplets on green orange leaves, but also has the characteristics of low cost and environmental friendliness, and has a very good application prospect.

[0142] The above has described the present invention in detail. For those skilled in the art, without departing from the gist and scope of the present invention and without the need for unnecessary experiments, the present invention can be implemented within a relatively wide range under equivalent parameters, concentrations and conditions. Although the present invention gives specific embodiments, it should be understood that the present invention can be further improved. In short, according to the principle of the present invention, this application intends to cover any modifications, uses or improvements of the present invention, including those that depart from the scope disclosed in this application but are made with conventional techniques known in the art. Some basic features can be applied according to the scope of the appended claims below.

Claims

1. Use of a spreading agent for green oranges with polyoxyethylene sorbitan trioleate and sodium dodecylbenzenesulfonate as active ingredients in improving the efficacy of a medicament on green oranges, wherein the medicament is a medicament that can be applied to green oranges by spraying; The spreading agent for green oranges is prepared by the following method, which includes: Mix polyoxyethylene sorbitan trioleate, dimethyl silicone oil with two products of Shanghai Kao Corporation with catalog numbers NEOPELEX G-30 and NEOPELEX G-20 respectively, and carry out homogenization to obtain the spreading agent for green oranges, wherein the mass ratio of polyoxyethylene sorbitan trioleate, dimethyl silicone oil to the two products of Shanghai Kao Corporation with catalog numbers NEOPELEX G-30 and NEOPELEX G-20 respectively is 75:1:150:

25.

2. Use of a spreading agent for green oranges with polyoxyethylene sorbitan trioleate and sodium dodecylbenzenesulfonate as active ingredients in improving the fertilizer efficiency of a foliar fertilizer on green oranges; The spreading agent for green oranges is prepared by the following method, which includes: Mix polyoxyethylene sorbitan trioleate, dimethyl silicone oil with two products of Shanghai Kao Corporation with catalog numbers NEOPELEX G-30 and NEOPELEX G-20 respectively, and carry out homogenization to obtain the spreading agent for green oranges, wherein the mass ratio of polyoxyethylene sorbitan trioleate, dimethyl silicone oil to the two products of Shanghai Kao Corporation with catalog numbers NEOPELEX G-30 and NEOPELEX G-20 respectively is 75:1:150:

25.

3. Method for improving the efficacy of a medicament on green oranges, characterized in that: The method includes formulating the medicament into a spray solution, adding the spreading agent for green oranges to the spray solution to obtain a spray solution containing the spreading agent for green oranges, and then applying the spray solution containing the spreading agent for green oranges to green oranges by spraying to improve the efficacy of the medicament on green oranges; the medicament is a medicament that can be applied to green oranges by spraying; The spreading agent for green oranges is prepared by the following method, which includes: mixing polyoxyethylene sorbitan trioleate, dimethyl silicone oil with two products of Shanghai Kao Corporation with catalog numbers NEOPELEX G-30 and NEOPELEX G-20 respectively, and carrying out homogenization to obtain the spreading agent for green oranges, wherein the mass ratio of polyoxyethylene sorbitan trioleate, dimethyl silicone oil to the two products of Shanghai Kao Corporation with catalog numbers NEOPELEX G-30 and NEOPELEX G-20 respectively is 75:1:150:

25.

4. Method for improving the fertilizer efficiency of foliar fertilizer on green oranges, characterized in that: The method includes formulating the foliar fertilizer into a spray solution, adding the spreading agent for green oranges to the spray solution to obtain a spray solution containing the spreading agent for green oranges, and then applying the spray solution containing the spreading agent for green oranges to green oranges by spraying to improve the fertilizer efficiency of the foliar fertilizer on green oranges; The spreading agent for green oranges is prepared by the following method, which includes: mixing polyoxyethylene sorbitan trioleate, dimethyl silicone oil with two products of Shanghai Kao Chemical Co., Ltd. with catalog numbers NEOPELEX G-30 and NEOPELEX G-20 respectively for homogenization to obtain the spreading agent for green oranges, wherein the mass ratio of polyoxyethylene sorbitan trioleate, dimethyl silicone oil to the two products of Shanghai Kao Chemical Co., Ltd. with catalog numbers NEOPELEX G-30 and NEOPELEX G-20 respectively is 75:1:150:25.

Images