Methods for promoting the revival, germination and reproduction of dry peat moss

Abstract

This application provides a method for promoting the revival, germination, and propagation of dried peat moss, comprising: selecting dried peat moss as a culture material and pre-treating the dried peat moss; and cultivating the dried peat moss according to preset culture conditions until filaments sprout from the dried peat moss. This application solves the technical problems of difficult preservation of peat moss propagation materials, a complex propagation process, and low propagation efficiency.

Description

Technical Field

[0001] The present application relates to the technical field of peat moss propagation, and in particular to a method for promoting the revival, germination and propagation of dry peat moss. Background Art

[0002] Global warming is the most severe environmental challenge facing humanity. Effectively controlling carbon emissions and fully leveraging the carbon sequestration capacity of ecosystems are important means to achieve carbon neutrality.

[0003] Among the current charcoal-forming peat mosses, Sphagnum may be the species that stores the most carbon. Although the individual Sphagnum is small, it covers more than 1.5×108hm 2 of the Earth's land surface, accounting for 1% of the land area. More than half of the world's peat comes from Sphagnum mosses, which account for 10%–15% of terrestrial carbon storage.

[0004] Sphagnum moss, the primary component of peatlands, has a higher carbon sequestration capacity than any other terrestrial peat moss. Due to its carbon sequestration, carbon storage, water storage, and acidification properties, peat moss, as an "effective ecosystem engineer," possesses unique and irreplaceable ecological value in peatland carbon sequestration, freshwater filtration, soil conditioning, and flood protection. It is crucial for ecosystem maintenance and global climate regulation.

[0005] Through the cultivation and planting of peat moss, renewable peat moss can be used to replace peat, thereby mitigating global warming and repairing damaged peatland ecosystems. With the in-depth research and utilization of its chemical composition, peat moss has also gained important application value in industries such as medicine, high-end agricultural substrates, soil conditioning, ecological pollution monitoring, and heavy metal pollution control. It is a carbon sequestration peat moss with significant ecological, economic, and scientific value.

[0006] However, peat moss has traditionally been primarily harvested from wild resources, severely damaging the peatland ecosystem. There is an urgent need to reintroduce peat moss to the wild and restore peatlands. Both peatland restoration and high-value applications require artificial propagation, but the current bottlenecks in peat moss cultivation lie in the availability, lifespan, and cost of seedlings. Therefore, achieving rapid artificial propagation of peat moss has become a research hotspot across various countries.

[0007] There are currently two ways to propagate peat moss: one is to use spores for germination culture using tissue culture medium. However, this method makes spores difficult to obtain, the procedure is complicated, and the cost of seedling cultivation is high; the other is to use fresh peat moss gametophytes for asexual reproduction. Asexual reproduction using 5-10 cm fresh peat moss gametophytes or 1-2 cm peat moss gametophyte heads as reproductive bodies is the most effective, but after the gametophyte head is removed, the remaining part is difficult to survive, difficult to recover, the reproduction rate is low, and the reproduction material is difficult to preserve.

[0008] In summary, the propagation materials used in the above two propagation methods are all fresh materials, the quantity of propagation materials is small, and they are not easy to preserve. Obtaining propagation materials will cause great damage to wild resources.

[0009] To address the above issues, no effective solutions have been proposed so far. Summary of the Invention

[0010] The embodiments of the present application provide a method for promoting the revival, germination and reproduction of dry peat moss, so as to solve the technical problems in the prior art of difficult preservation of peat moss propagation materials, complex propagation process and low propagation efficiency.

[0011] According to one aspect of an embodiment of the present application, a method for promoting the revival, germination and reproduction of dry peat moss is provided, comprising: selecting dry peat moss as a culture material and pretreating the dry peat moss; cultivating the dry peat moss according to preset culture conditions until the dry peat moss germinates into filaments.

[0012] Existing methods for growing dry peat moss all use living peat moss material (fresh peat moss) or seed-like propagation materials such as spores. This propagation method has technical problems such as difficulty in preserving fresh peat moss or spores, a complex propagation process, and low propagation efficiency. However, the present application uses dry peat moss as the culture material, pre-treats the dry peat moss, and then cultivates the dry peat moss according to preset culture conditions until the dry peat moss sprouts filaments, thereby solving the above-mentioned problems of the existing technology and achieving the beneficial effects of easy preservation of the propagation material, a simple propagation process, and high propagation efficiency. BRIEF DESCRIPTION OF THE DRAWINGS

[0013] The drawings that constitute part of this application are used to provide a further understanding of this application. The illustrative embodiments of this application and their descriptions are used to explain this application and do not constitute an improper limitation on this application. In the drawings:

[0014] Figure 1 This is a flow chart of a method for promoting the revival, germination and reproduction of dry peat moss according to an embodiment of the present application;

[0015] Figure 2is a flow chart of another method for promoting the revival, germination and reproduction of dry peat moss according to an embodiment of the present application;

[0016] Figure 3 is a flow chart of a method for cultivating dry peat moss according to preset culture conditions according to an embodiment of the present application;

[0017] Figure 4 This is a flow chart of another method for promoting the revival, germination and reproduction of dry peat moss according to an embodiment of the present application;

[0018] Figure 5 is a schematic diagram of selected dry peat moss according to an embodiment of the present application;

[0019] Figure 6 is a schematic diagram of peat moss after being placed in a bed according to an embodiment of the present application;

[0020] Figure 7 is a schematic diagram of covered peat moss according to an embodiment of the present application;

[0021] Figure 8 is a schematic diagram of peat moss in a moist air humidity state according to an embodiment of the present application;

[0022] Figure 9 is a schematic diagram of peat moss in an initial germination state according to an embodiment of the present application;

[0023] Figure 10 is a schematic diagram of sphagnum moss sprouting a large number of filaments according to an embodiment of the present application;

[0024] Figure 11 is a schematic diagram of sphagnum moss forming a large number of gametophytes according to an embodiment of the present application;

[0025] Figure 12 is a schematic diagram of a bagged seedling according to an embodiment of the present application;

[0026] Figure 13 is a schematic diagram of the peat moss remaining after the plates are divided according to an embodiment of the present application;

[0027] Figure 14 This is a schematic diagram of continuing to cultivate and germinate the remaining peat moss after the trays are divided according to an embodiment of the present application;

[0028] Figure 15 It is a schematic diagram of a tray seedling according to an embodiment of the present application. DETAILED DESCRIPTION

[0029] It should be noted that, in the absence of conflict, the embodiments and features of the embodiments in this application can be combined with each other. The present application will be described in detail below with reference to the accompanying drawings and in combination with the embodiments.

[0030] It should be noted that the terms used herein are only for describing specific embodiments and are not intended to limit the exemplary embodiments according to the present application. As used herein, unless the context clearly indicates otherwise, the singular form is also intended to include the plural form. In addition, it should be understood that when the terms "comprise" and / or "include" are used in this specification, they indicate the presence of features, steps, operations, devices, components and / or combinations thereof.

[0031] Unless otherwise specifically stated, the relative arrangement of the parts and steps, numerical expressions and numerical values set forth in these embodiments do not limit the scope of the present application. At the same time, it should be understood that, for ease of description, the sizes of the various parts shown in the drawings are not drawn according to actual proportional relationships. The techniques, methods and equipment known to those of ordinary skill in the relevant art may not be discussed in detail, but where appropriate, the techniques, methods and equipment should be considered as part of the authorization specification. In all examples shown and discussed here, any specific values should be interpreted as being merely exemplary and not as limitations. Therefore, other examples of the exemplary embodiments may have different values. It should be noted that similar numbers and letters represent similar items in the following figures, and therefore, once an item is defined in one figure, it does not need to be further discussed in subsequent figures.

[0032] Sphagnum mosses are a type of moss with unique morphological, physiological, biochemical, and developmental characteristics. The gametophyte stage of Sphagnum mosses can be divided into the protonema and gametophyte stages. Both filamentous protonema and rhizoid-like structures with chloroplasts beginning to degenerate have the potential to form secondary leaf-like protonema.

[0033] Reproduction is essential for species survival and population development. To achieve sustainable survival and reproduction, peat mosses in nature constantly develop unique reproductive properties through adaptation to their environment. Extensive research on the reproductive methods of peat mosses in the wild indicates that peat mosses, primarily sphagnum mosses, in peatlands primarily achieve continuous population renewal through asexual reproduction, while also retaining sexual reproduction. Therefore, artificial asexual reproduction of peat mosses is feasible.

[0034] Example 1

[0035] The present invention provides a method for promoting the revival, germination and reproduction of dry peat moss. Figure 1 As shown, the method includes the following steps:

[0036] Step S101, selecting high-quality dry peat moss as a culture material.

[0037] The culture material is clean dry peat moss with a thickness of 6-10 mm and good elasticity.

[0038] Step S102: pre-treating the dry peat moss.

[0039] Rehydrate the selected dry peat moss, place half the volume of the container into a plastic container, and then add 1 / 3 of the volume of the dry peat moss to fully soak it for 1 day. In some examples, the clean water can also be replaced with a 60-80 mg / L GA3 aqueous solution. The 60-80 mg / L GA3 aqueous solution is a liquid containing gibberellin (GA3) hormone, wherein the concentration of GA3 is 60-80 mg per liter of aqueous solution. 60-80 mg / L GA3 can promote the growth and development of dry peat moss, and improve the stress resistance of dry peat moss. At the same time, it can also be used for growth regulation of dry peat moss and accelerate the germination of dry peat moss filaments.

[0040] In other embodiments, aqueous solutions of other formulations may be used to soak dry peat moss, for example, a chelated trace element solution containing trace elements such as iron, zinc, manganese, copper, molybdenum, and boron, wherein iron accounts for 25%, zinc accounts for 10%, manganese accounts for 10%, copper accounts for 5%, molybdenum accounts for 2%, and boron accounts for 1%. Alternatively, an organic nitrogen solution containing organic nitrogen compounds such as humic acid and amino acids, accounting for 30% of the total mass. Alternatively, potassium dihydrogen phosphate, accounting for 10% of the total mass. Alternatively, a bioactive substance containing bioactive substances such as gibberellins, auxins, phytases, and ligninases, accounting for 20% of the total mass. Alternatively, an organic acid solution containing organic acids such as malic acid, tartaric acid, and citric acid, accounting for 10% of the total mass.

[0041] Using these nutrient solutions to soak dry peat moss can provide the necessary nutrients and hormones required for growth. Specifically, the chelated trace element solution contains a variety of trace elements that can provide the iron, zinc, manganese, copper, molybdenum, boron and other elements required by plants. The organic nitrogen solution contains organic nitrogen compounds such as humic acid and amino acids, which can provide the nitrogen required by plants. Potassium dihydrogen phosphate can provide the phosphorus required by plants, and the biologically active substances contain gibberellins, auxins, phytases, ligninases and other biologically active substances that can stimulate the growth of dry peat moss. The organic acid solution contains organic acids such as malic acid, tartaric acid, and citric acid, which can help plants absorb nutrients and promote their growth.

[0042] Step S103, controlling the rehydration humidity of the dry peat moss.

[0043] Take out the fully soaked peat moss and squeeze out excess water to make its moisture content at 78-80% or squeeze it with your hands without water dripping. After that, the humidity of the dry peat moss is also maintained in this range during the cultivation process. The squeezed water and peat moss residue are kept for subsequent use.

[0044] Step S104: controlling the air humidity of the peat moss culture.

[0045] The rehydrated peat moss is spread into an incubator with a transparent lid to a thickness of 5-8 cm and then covered with the lid. The humidity in the incubator is maintained at 85-90% during the incubation period. Approximately 7 days later, the water squeezed out of step S103 and the remaining peat moss are poured into the incubator, a small amount being sufficient to keep the peat moss moist. Thereafter, every 3-4 days, the transparent lid is removed to allow ventilation for 1 hour, and water is sprayed with mist for 3-10 seconds to keep the peat moss moist. The transparent lid is then replaced.

[0046] Step S105, controlling the temperature of the peat moss cultivation.

[0047] The culture temperature in the incubator is maintained at 12°C-28°C, wherein the night temperature is 12-16°C and the day temperature is 20-28°C.

[0048] Step S106, controlling the lighting conditions of the culture.

[0049] Cultivate in a greenhouse with both outer and inner shades. Keep the outer shades fully open throughout the entire cultivation process, and the inner shades open from the time the peat moss is placed until the filaments germinate. Place the incubator on a shaker with slanting sunlight in the morning and evening, if possible. During the subculture stage, open the inner shades 2 / 3 in winter and fully in summer, maintaining a light intensity of 2000-15000 lux.

[0050] Step S107: perform transplantation.

[0051] Repeat the above steps S103 to S106 for 2-3 months until the peat moss germinates new filaments. Then increase the number of mist spraying times and spray once every 2-3 days until about 80% of the peat moss in the entire incubator has germinated. Move the germinated peat moss to the peat moss treated according to steps S101-S103 and spread it flat. Then transplant it to the newly treated dry peat moss as it is and continue to cultivate it. After transplanting the germinated peat moss, continue to cultivate the remaining peat moss until it germinates, and then transplant it again. Repeat this process until all of it has germinated completely.

[0052] In some embodiments, in addition to water spraying, a 0.1 g / l 6-benzylaminoadenine + 0.2 g / l naphthaleneacetic acid solution may be sprayed three times every seven days. The 0.1 g / l 6-benzylaminoadenine + 0.2 g / l naphthaleneacetic acid solution is prepared by dissolving 6-benzylaminoadenine and naphthaleneacetic acid in water at concentrations of 0.1 g / l and 0.2 g / l, respectively. This solution can be sprayed onto plant leaves to promote germination of peat moss.

[0053] In other embodiments, the following solutions can be sprayed every 7 days. For example, a 0.1 g / L gibberellin (GA3) + 0.2 g / L indole-3-butyric acid (IBA) solution; or a 0.2 g / L 6-benzylaminoadenine (6-BA) + 0.1 g / L naphthaleneacetic acid (NAA) solution; or a 0.3 g / L kinetin + 0.1 g / L indole-3-acetic acid (IAA) solution; or a mixture of the above solutions. Gibberellin can promote filament germination, growth, and branching of filaments; indole-3-butyric acid can promote filament growth; 6-benzylaminoadenine promotes the formation of lateral buds of filaments; naphthaleneacetic acid can promote filament formation; kinetin can promote filament cell division and flower bud formation; and indole-3-acetic acid can promote filament growth and development. These solutions can be mixed together in the above proportions to form a composite liquid to meet the needs of filaments at different growth stages.

[0054] Step S108, subculture.

[0055] The newly germinated sphagnum moss filaments continue to be cultured according to the culture conditions of steps S103-S105, and a heating pad is placed under the incubator to maintain the temperature at 19-20°C, ensuring that the culture temperature is not lower than 10°C, and a 1.5‰ urea aqueous solution is sprayed in a mist form, spraying once every 10 days, and spraying continuously for 3-4 times. When the incubator is full, the trays are divided, and this process can be repeated.

[0056] In some embodiments, 1.5‰ urea + 1.5‰ potassium dihydrogen phosphate aqueous solution can also be sprayed in a mist form, once every 10 days, and after spraying continuously for 3-4 times, 0.15g / l 6-benzylaminoadenine solution can be sprayed in a mist form for 3 consecutive times. When the incubator is fully grown, the trays can be divided.

[0057] Spraying a 1.5‰ urea + 1.5‰ potassium dihydrogen phosphate aqueous solution helps provide the nutrients necessary for the filaments. Urea is the primary nitrogen source for filamentous growth, while potassium dihydrogen phosphate is the primary source of phosphorus and potassium. Therefore, this aqueous solution provides the necessary nutrients for peat moss, promoting its growth and development. Furthermore, potassium dihydrogen phosphate improves the resistance and adaptability of peat moss, making it more adaptable to cultivation conditions and enhancing its growth capacity.

[0058] This application is completely different from the materials and methods used in current peat moss culture. It uses dry peat moss instead of fresh peat moss or fresh spores as the culture material, overcoming the high cost, inconvenient management, and difficult preservation problems of fresh peat moss germination or spore germination culture. Specifically, it is embodied in:

[0059] 1) Using dry peat moss for cultivation, the material is easy to obtain and preserve, which is particularly conducive to the preservation and breeding of high-quality germplasm resources, and can be propagated at any time according to needs;

[0060] 2) The required facilities are simple and easy to meet;

[0061] 3) The cultivation method is simple, the seedling propagation rate is high and the cost is low;

[0062] 4) The cultured seedlings are clean and free of impurities, pollution or weeds, and can be used as the preferred way to obtain explant materials for tissue culture.

[0063] Example 2

[0064] Traditional peat moss cultivation involves harvesting wild peat moss and planting it in farmland with adequate water levels. However, this method consumes a significant amount of wild peat moss, which grows very slowly, averaging approximately 3.6 cm (0.4–12.9 cm) per year in the wild, depending on the species and environment. Overharvesting can lead to ecological damage in its native habitat and excessive greenhouse gas emissions. Therefore, obtaining a sufficient supply of peat moss seedlings is crucial for peatland restoration and its widespread use.

[0065] The embodiment of the present application provides another method for promoting the revival, germination and reproduction of dry peat moss. The embodiment of the present application only requires the peat moss to be dried and stored, and revived when needed, thus effectively solving the bottleneck problem of peat moss germplasm resource preservation and rapid seedling breeding. Figure 2 As shown, the method includes:

[0066] Step S202: Select dry peat moss as a culture material and pre-treat the dry peat moss.

[0067] In some exemplary embodiments, dry peat moss with a stem size between 6 and 10 mm and an elasticity greater than a preset elasticity threshold is selected as the culture material; the dry peat moss is placed in a container, and a GA3 aqueous solution is added to the container at a preset volume multiple of the volume of the dry peat moss to soak the dry peat moss so that the dry peat moss is rehydrated, wherein the preset volume multiple is 1 / 3, and the volume of the dry peat moss occupies half the volume of the container.

[0068] In the present embodiment, the dry peat moss with stem size between 6 to 10 millimeters and good elasticity is selected as culture material, which can ensure the quality and availability of dry peat moss, thereby can provide a good growth basis for the breeding of dry peat moss, and such dry peat moss can keep enough air permeability and water retention, and can provide enough support to the growth of peat moss. In addition, dry peat moss is put into a container, and the GA3 aqueous solution that adds a preset volume multiple is soaked, which can help dry peat moss to be rehydrated, provides the required moisture and nutrient for peat moss breeding, and is conducive to peat moss germination and growth. Simultaneously, the volume multiple of immersion is 1 / 3 also in order to keep suitable moisture and air content. In general, this pre-treatment operation helps to provide an environment that is suitable for peat moss growth, so that peat moss can better absorb moisture and nutrient, while keeping enough air permeability and support.

[0069] After rehydrating the dried peat moss, the rehydrated moisture of the peat moss is maintained between 78% and 80%, and the rehydrated peat moss is spread flat in the first incubator with a transparent lid. This allows the peat moss to recover moisture, providing it with the necessary moisture and nutrients for growth, and at the same time, helps promote the growth and development of the peat moss.

[0070] Step S204 , cultivating the dry peat moss according to preset culture conditions until the dry peat moss sprouts filaments.

[0071] In some examples, the method of cultivating dry peat moss according to predetermined cultivation conditions is as follows: Figure 3 As shown, the method includes the following steps:

[0072] Step S2040, controlling the cultivation conditions.

[0073] In this embodiment, the water holding capacity of the rehydrated peat moss and the air humidity, light, temperature, etc. during cultivation are controlled to achieve the germination of the peat moss into new protonema or gametophyte.

[0074] 1) Control the humidity of peat moss;

[0075] After a preset period of time, the squeezed water and the remaining peat moss are poured into the peat moss in the first incubator to maintain the peat moss in a moist state. Before the peat moss sprouts filaments, the humidity of the peat moss is maintained between 78% and 80%. In this embodiment, pouring the squeezed water and the remaining peat moss into the peat moss in the first incubator helps maintain moisture and nutrients in the incubation environment, promotes nutrient and water recycling, avoids waste and pollution, and improves resource utilization efficiency.

[0076] 2) Maintain air humidity.

[0077] At predetermined intervals, the transparent lid is opened, air is ventilated for a predetermined ventilation period, and water is sprayed in a mist form for 3 to 10 seconds to keep the peat moss moist. The transparent lid is then closed. In this embodiment, the first incubator is provided with a transparent lid to maintain air humidity within the incubator. Ventilation and water spraying during the predetermined ventilation period facilitate maintaining humidity and nutrient supply within the incubator, thereby promoting the growth and development of the dry peat moss. In this embodiment, air humidity is typically maintained between 85% and 90%. Providing appropriate humidity and gas exchange promotes the growth of the peat moss.

[0078] In some embodiments, the preset period is set to 7 days, the preset interval is set to 3 or 4 days, and the preset ventilation period is set to 1 hour. Such setting helps to maintain the appropriate humidity and ventilation state of peat moss to promote the growth and development of dry peat moss. Specifically, setting the preset period to 7 days helps to ensure that peat moss is fully grown and developed in a sufficiently long time to achieve the purpose of sprouting filaments. Setting the preset interval to 3 or 4 days helps to maintain the humidity and ventilation state of peat moss to avoid excessive drying or hypoxia, and also can better manage and monitor the growth of peat moss. Setting the preset ventilation period to 1 hour helps to promote ventilation and gas exchange of peat moss to maintain appropriate humidity and gas composition, and also helps to prevent the growth of pathogens and fungi. In summary, the settings of these preset periods and intervals can help create an environment that is conducive to the growth and development of peat moss to improve the success rate of dry peat moss sprouting filaments.

[0079] 3) Control the temperature inside the incubator.

[0080] The temperature of the first incubator for cultivating the dry peat moss is controlled between 12° C. and 28° C., wherein the temperature at night is between 12° C. and 16° C., and the temperature during the day is between 20° C. and 28° C. This can provide suitable growth temperature and environmental conditions for the peat moss.

[0081] 4) Control lighting.

[0082] Place the first incubator on a shaker with slanting sunlight in the morning and evening. This will help enhance the growth and adaptability of the peat moss. Dried peat moss is sensitive to light and requires a certain amount of light to germinate. Slanting sunlight can stimulate germination and growth, promote photosynthesis, and enhance the dry peat moss's adaptability to the environment. It also helps maintain the stability of environmental parameters such as humidity and temperature.

[0083] In addition, it is necessary to keep the outer shading and inner shading of the greenhouse accommodating the first incubator fully open. Like this, help to provide suitable lighting conditions, for the growth of dry peat moss provides necessary photosynthesis conditions.

[0084] Step S2042: determining whether sphagnum moss has germinated into filaments.

[0085] If filaments sprout, execute step S2044; otherwise, jump to step S2040.

[0086] Step S2044, multiple mist spraying and hormone treatment.

[0087] Increase the frequency of mist spraying, misting once every 2 to 3 days. Increasing the frequency of mist spraying can help maintain the humidity of the filaments, provide the water they need, and promote their growth. At the same time, mist spray 0.1g / l 6-benzylaminoadenine + 0.2g / l naphthaleneacetic acid solution at intervals of 7 days for 3 consecutive times to promote increased filament reproduction, increase branching, and improve filament germination rate.

[0088] In some embodiments, the hormone formulation can be as follows: 100 ppm jasmonic acid and 0.5 ppm gibberellin. Jasmonic acid can promote conchocelis growth and development, promote conchocelis branching and lateral bud growth. Gibberellin can promote conchocelis growth and development, improve conchocelis stress resistance and adaptability, and enhance conchocelis photosynthesis.

[0089] This hormone formula can be sprayed once every 2 to 3 days, and 0.1g / l 6-benzylaminoadenine + 0.2g / l naphthaleneacetic acid solution can be added every 7 days for 3 consecutive sprays. This can promote the growth and branching of filaments and increase the germination rate. Gibberellic acid can promote the growth and development of filaments and enhance their adaptability and stress resistance. Continuous spraying of 6-benzylaminoadenine and naphthaleneacetic acid can promote the reproduction and branching of filaments and increase their quantity and quality. In summary, this hormone formula can improve the growth and development of peat moss, increase the quantity and quality of filaments, and contribute to the reproduction and cultivation of peat moss.

[0090] Step S2046: determine whether the filamentous body sprouting rate exceeds 80%.

[0091] If it does not exceed 80%, jump to step S2044; otherwise, execute step S2047.

[0092] Step S2047, transplanting the germination layer.

[0093] The germinated filaments of the sphagnum moss are transplanted to the second incubator for further cultivation. For the first incubator, step S2048 is performed, and for the second incubator, step S2049 is performed.

[0094] Step S2048: Determine whether there is any remaining peat moss in the first incubator.

[0095] If there is still dry peat moss remaining in the first incubator, the process jumps to step S2040 to continue incubation; otherwise, the incubation process in the first incubator ends.

[0096] Step S2049, subculture.

[0097] A heating pad is placed under the second incubator to maintain the temperature of the heating pad within a preset temperature range. A urea + potassium dihydrogen phosphate aqueous solution of a preset concentration is sprayed in a mist form once every preset number of days. After spraying continuously for 3 to 4 times, a 0.15 g / l 6-benzylaminoadenine solution is sprayed in a mist form for 3 consecutive times to promote the growth and reproduction of peat moss.

[0098] Transplanting the germinated peat moss into a second incubator and using a heating pad to maintain the temperature can provide the required temperature conditions, which helps maintain the growth and development of the filaments. Using a mist spray to spray urea + potassium dihydrogen phosphate aqueous solution can provide the required water and nutrients for the filaments, promoting their growth. Mist spraying of 6-benzylaminoadenine solution can promote the proliferation of filaments. At the same time, spraying urea + potassium dihydrogen phosphate aqueous solution at intervals can prevent problems such as excessive spraying that may cause fertilizer damage to peat moss or inhibit growth. Therefore, these operations can improve the growth quality and yield of peat moss, which is beneficial to production and research applications.

[0099] In some embodiments, the temperature of the heating pad can be maintained within a preset range of 19°C to 20°C, which can provide the most suitable growth environment for the filaments and contribute to their healthy growth. In addition, mist spraying using urea + potassium dihydrogen phosphate aqueous solution can provide nutrients for the filaments and promote their growth and development. Spraying a preset concentration of urea + potassium dihydrogen phosphate aqueous solution once every preset number of days, and spraying continuously 3 to 4 times, can provide sufficient nutrition for peat moss, and the preset concentration of 1.5‰ can ensure that the nutrient concentration is appropriate and will not cause over-fertilization. After fertilization, mist spraying of 6-benzylaminoadenine solution can promote the proliferation of filaments, increase branches, and promote the rapid growth of peat moss.

[0100] The culture conditions in the subculture stage are similar to those in step S2040, with the difference being the lighting conditions. During subculture, the inner shading is opened 2 / 3 in winter and fully in summer. This is because in winter, the inner shading of the greenhouse equipped with the second incubator is partially closed to increase light transmission, which can have a heat-insulating effect, avoiding the adverse effects of low temperatures on the growth of filaments, thereby facilitating the growth of filaments. In summer, opening the inner and outer shading can lower the temperature in the greenhouse, which is conducive to the growth of filaments.

[0101] This application adopts a method different from the current peat moss breeding method, using dried peat moss for resurrection and regeneration breeding, which effectively solves the problem of preservation of breeding materials. At the same time, it is simple to operate, easy to expand and propagate, and has a high breeding rate, providing an important way for the efficient breeding of peat moss seedlings.

[0102] Example 3

[0103] Currently, peat moss seedlings are primarily obtained from three sources: 1) establishing a sterile in vitro culture system from wild spores to obtain sterile seedlings, but this has not yet reached the production stage; 2) cultivating peat moss primarily by digging up wild peat moss and planting it in the field; and 3) obtaining seedlings through asexual propagation using wild peat moss gametophytes. The gametophytic asexual propagation method involves using 5–10 cm fresh peat moss gametophytes or 1–2 cm peat moss gametophyte heads as propagules.

[0104] However, these methods all have problems such as difficulty in preserving sphagnum moss propagation materials, high costs, serious damage to wild resources, low propagation rates, and complex operations. Rapid propagation technology for sphagnum moss is still a bottleneck in the development of the sphagnum moss cultivation industry.

[0105] In order to solve the above problems, the present invention provides another method for promoting the revival, germination and reproduction of dry peat moss. Figure 4 As shown, the method includes:

[0106] Step S401: Select dry peat moss and soak it for rehydration.

[0107] In order to obtain high-quality culture materials, such as Figure 5 As shown, this embodiment selects dry peat moss with a thickness of 6-10mm, good elasticity, and cleanness as the raw material. In the process of preparing the culture material, the dry peat moss needs to be rehydrated first. Specifically, the dry peat moss is placed in a plastic container, and the dry peat moss can be filled to half the container's volume. Then, clean water is added to 1 / 3 of the volume of the dry peat moss and fully infiltrated for 1 day. This ensures that the culture material fully absorbs water while avoiding the use of too much water. This preparation method can produce high-quality culture material, providing a solid foundation for subsequent propagation.

[0108] Step S402: Place the bed.

[0109] To prepare the culture environment, lay the rehydrated peat moss in a culture box with a transparent lid. Figure 6 As shown, the thickness of the peat moss should be 5-8 cm, ensuring it is laid evenly.

[0110] Step S403: adding a cover.

[0111] like Figure 7As shown, after the laying is completed, the incubator needs to be covered with a transparent plastic lid to ensure that the temperature and humidity of the environment are controlled. This preparation method can provide a stable and comfortable growth environment for subsequent peat moss cultivation.

[0112] Step S404: maintaining the preset culture conditions.

[0113] During the cultivation process, Figure 8 As shown, the air humidity in the incubator needs to be maintained within the range of 85-90%. After 7 days of bed placement, the water squeezed out in step S401 and the remaining peat moss are poured into the incubator. Each time watering, a moderate amount should be poured to keep the peat moss moist between 78% and 80%. Thereafter, the lid needs to be opened every 3-4 days for ventilation for 1 hour, and a mist spray is performed for 3-10 seconds to keep the peat moss moist. Afterwards, the lid is replaced. This method can provide a stable humidity environment for the subsequent growth of peat moss.

[0114] In addition, when using dry peat moss for cultivation, the cultivation temperature needs to be maintained between 12°C and 28°C. The night temperature should be 12-16°C, while the daytime temperature should be maintained at 20-28°C. In addition, the cultivation process requires certain lighting conditions, maintaining a light intensity of 2000-15000 lux. Cultivation can be carried out in a greenhouse with external shading and internal shading. During the entire cultivation process, the external shading needs to be kept fully open. The internal shading needs to be opened during the period from the time the peat moss is placed in the bed to the time the filaments germinate. In addition, the incubator needs to be placed on a shaker with oblique sunlight in the morning and evening to provide appropriate lighting conditions.

[0115] Step S405, start germination.

[0116] After the above step S404 is continued for 2 to 3 months, the dry peat moss sprouts new filaments, such as Figure 9 shown.

[0117] Step S406: sprouting a large number of filaments.

[0118] After the peat moss sprouts filaments, the frequency of mist spraying needs to be increased. It is recommended to spray once every 2-3 days until about 80% of the peat moss in the entire incubator has sprouted. Figure 10 In this embodiment, the number of mist spraying is increased, which can provide sufficient water and humidity for the peat moss while maintaining the stability of the culture environment.

[0119] Step S407: dividing the filament into disks.

[0120] After about 80% of the peat moss in the incubator has germinated into filaments, the peat moss is divided into trays, and the surface layer of the peat moss (i.e., the germination layer where the filaments have germinated) is divided into trays and placed in another incubator.

[0121] After the trays are divided, the process returns to step S404 for the remaining peat moss in the original incubator and continues to culture until filaments germinate; and the process proceeds to step S408 for the filaments transplanted into the new trays.

[0122] Step S408: forming a large number of gametophytes.

[0123] Sphagnum moss is a type of moss that grows in peat bogs and wetlands. Its filaments usually refer to the asexual reproductive organs of Sphagnum moss - the reproductive filaments. The reproductive filaments of Sphagnum moss are composed of filamentous cells that start from the axils of leaves and grow outward and branch out to form a spider web-like structure. If the filaments are further cultivated, the ends of the reproductive filaments will differentiate into female or male gametophytes, forming a large number of gametophytes, such as Figure 11 shown.

[0124] The gametophyte is the reproductive organ of dry sphagnum moss, developing from fertilized eggs within the conchocelis. The gametophyte is typically smaller and morphologically distinct from the conchocelis. Its primary function is to produce spores, which disperse and grow into new conchocelis. Unlike conchocelis, the gametophyte lacks chlorophyll, cannot photosynthesize, and lacks roots to absorb water and nutrients.

[0125] After a large number of gametophytes are formed, step S409 may be performed, or step S410 may be performed, or steps S409 and S410 may be performed simultaneously.

[0126] Step S409, bagging seedlings.

[0127] The gametophytes are cultivated in the form of bag seedlings, such as Figure 12 As shown. The gametophytes are further cultivated through bag seedling cultivation. Bag seedling cultivation is a seedling cultivation technique that involves cultivating the seedlings in specialized bags. Compared to traditional potted seedling cultivation methods, bag seedling cultivation offers several advantages, including a shorter seedling cycle, faster seedling growth, improved air permeability, and a higher survival rate. This process concludes after bag seedling cultivation.

[0128] Step S410, dividing the gametophytes into discs.

[0129] The large number of gametophytes formed are divided into plates, and the surface gametophytes are divided into new plates to form Figure 15 Seedlings in trays shown.

[0130] After the gametophytes are divided, Figure 13 For the gametes remaining after the separation, step S411 is executed. For the gametes transplanted into the new dish, step S412 is executed.

[0131] Step S411, continue culturing the remaining gametophytes.

[0132] Continue to cultivate the remaining gametophytes after the division to allow them to continue germinating, such as Figure 14 shown.

[0133] Step S412, forming seedling trays.

[0134] After the gametophytes are cultured, the following Figure 15 The gametophytes transplanted into the new tray are directly formed into tray seedlings. After forming the tray seedlings, continue to cultivate and jump to step S408 to form a large number of gametophytes, and continue to divide the trays for reproduction and repeat.

[0135] The present application is completely different from the materials and methods currently used for peat moss cultivation. Dry peat moss is used instead of fresh peat moss or fresh spores as the cultivation material, overcoming the problems of high cost, inconvenient management, and difficult preservation in fresh peat moss germination or spore germination cultivation.

[0136] The above description is merely a preferred embodiment of the present application and is not intended to limit the present application. Various modifications and variations are possible for those skilled in the art. Any modifications, equivalent substitutions, or improvements made within the spirit and principles of the present application shall be included within the scope of protection of the present application.

Claims

1. A method for promoting the revival, germination and reproduction of dry peat moss, characterized in that: include: Selecting dry peat moss as a culture material and pre-treating the dry peat moss; Cultivating the pretreated dry peat moss according to preset culture conditions until filaments sprout from the dry peat moss; The method comprises selecting dry peat moss as a culture material and pre-treating the dry peat moss, comprising: selecting dry peat moss with a stem size between 6 and 10 mm and an elasticity greater than a preset elasticity threshold as the culture material; placing the dry peat moss in a container and adding an aqueous solution at a preset volume multiple of the dry peat moss to the container to soak the dry peat moss so that the dry peat moss is rehydrated, wherein the preset volume multiple is 1 / 3, and the volume of the dry peat moss occupies half the volume of the container; removing the fully soaked peat moss and squeezing out excess water to reduce its moisture content to 78-80%; and spreading the rehydrated dry peat moss flatly in a first incubator provided with a transparent lid. Wherein, cultivating the dry peat moss according to preset culture conditions until the dry peat moss sprouts filaments, including continuously performing the following steps until the dry peat moss sprouts filaments: maintaining the humidity of the rehydrated peat moss between 78% and 80%; maintaining the humidity of the air in which the peat moss is cultivated between 85% and 90%; controlling the temperature in the first incubator for cultivating the peat moss between 12°C and 28°C, wherein the temperature at night is between 12°C and 16°C, and the temperature during the day is between 20°C and 28°C; controlling the lighting conditions of the peat moss, and keeping the outer and inner sunshades of the greenhouse containing the first incubator fully open; After the dry peat moss is spread into a first incubator provided with a transparent lid, the method further comprises: after a preset period of time, pouring the squeezed aqueous solution and peat moss residue into the peat moss in the first incubator to keep the peat moss in a moist state, wherein the preset period of time is 7 days; The method comprises cultivating the dry peat moss according to preset culture conditions until the dry peat moss sprouts filaments, and further comprising: opening the transparent lid at preset intervals, ventilating the peat moss for a preset ventilation period, and spraying water in a mist form for 3 to 10 seconds to keep the peat moss moist, and then covering the transparent lid. The preset intervals are 3 or 4 days, and the preset ventilation period is 1 hour.

2. The method according to claim 1, characterized in that After the filaments germinate, the method further includes: increasing the frequency of mist spraying, mist spraying once every 2 to 3 days, until the germination rate of the peat moss on the surface layer in the first incubator exceeds 80%.

3. The method according to claim 2, characterized in that After the germination rate of the peat moss in the surface layer of the first incubator exceeds 80%, the method further includes repeating the following steps until all the peat moss in the first incubator is germinated: transplanting the germinated peat moss to a second incubator for further culturing, and continuing to culture the remaining peat moss in the first incubator until it germinates, wherein the second incubator contains rehydrated peat moss.

4. The method according to claim 3, characterized in that The germinated peat moss is transplanted into a second incubator for further cultivation, comprising: placing a heating pad under the second incubator, maintaining the temperature of the heating pad within a preset temperature range, and spraying a urea aqueous solution of a preset concentration onto the peat moss in the second incubator in a mist form at intervals of a preset number of days, for three to four consecutive times.

5. The method according to claim 4, characterized in that The preset temperature range is 19°C to 20°C, the preset number of days is 10 days, and the preset concentration is 1.5‰.

6. The method according to claim 4 or 5, characterized in that The germinated peat moss is transplanted into a second culture box for further culture, and the method further comprises: in winter, opening 2 / 3 of the inner sunshade of the greenhouse where the second culture box is provided, and in summer, fully opening the inner sunshade and the outer sunshade.

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