A process for converting ginsenosides into rare ginsenosides by microbial method

By combining microbial methods with Soxhlet extractor and cellulose microbacterium 72-3 fermentation, the extraction and conversion processes were optimized, solving the problems of low conversion rate and high pollution of existing rare ginsenosides, and realizing efficient and environmentally friendly preparation of rare ginsenosides.

CN116144727BActive Publication Date: 2026-06-12PANJIN INSPECTION & TESTING CENT +1
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Patent Information

Application Number
CN202211536832.6
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2022-12-02
Publication Date
2026-06-12
Estimated Expiration
2042-12-02

AI Technical Summary

Technical Problem

Existing technologies for converting rare ginsenosides are complex, have low recovery rates, low efficiency in producing the target product, and contain a large amount of pollutants. These technologies generally suffer from low conversion rates, large reagent consumption, complex product composition, and difficulty in separating the target product.

Method used

The process of converting ginsenosides into rare ginsenosides using a microbial method involves combining a Soxhlet extractor with organic acids, fermenting total ginsenosides using microbes 72-3, and purifying them using macroporous resin column and silica gel column. The ethanol concentration and oil bath temperature are optimized to achieve a green, environmentally friendly, and highly efficient conversion.

Benefits of technology

This improved the conversion rate and purity of rare ginsenosides Rh1 and F1, reduced the generation of pollutants, and achieved an efficient and environmentally friendly rare ginsenoside preparation process.

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Abstract

The application discloses a process for converting ginsenoside into rare ginsenoside by a microbial method, and relates to the technical field of ginsenoside, and comprises the following steps: step S1, taking ginseng rootlet powder, putting the ginseng rootlet powder into extraction cylinders of Soxhlet extractors in 10 times, adding an ethanol solution and an organic acid mixed solution into the extraction cylinders, and placing the extraction bottles in an oil bath at a certain temperature to carry out reflux extraction to obtain ginsenoside extraction crude powder; step S2, microbial conversion: adding the ginsenoside crude powder obtained in the step S1 into LB liquid culture medium, and adding fiber bacteria 72-3 bacterial liquid in a proportion of 0.5-2.0 %, and using the newly separated fiber bacteria 72-3 to ferment and convert the ginsenoside into rare ginsenoside, and first, the conversion is carried out through 48 hours of culture at 37 degrees; and step S3, further enrichment, adsorption, elution and filtration of the ginsenoside through a macroporous resin column and a silica gel chromatographic column, vacuum drying and concentration and crystallization, and finally, a mixture containing rare ginsenoside hydrolysis products Rh1 and F1 is obtained.
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Citation Information

Patent Citations

  • Method for preparing rare ginsenoside Rg2 through biotransformation

    CN104342381A

  • Method for preparing ginseng rare saponin by virtue of microorganism enzymatic conversion of ginsenosid Re

    CN104894204A