Use of saponin compounds in nucleic acid sequencing
JP7863615B2Active Publication Date: 2026-05-21MGI TECH CO LTD
Patent Information
- Application Number
- JP2024520960
- Authority / Receiving Office
- JP · JP
- Patent Type
- Patents
- Current Assignee / Owner
- Priority Date
- 2021-10-11
- Filing Date
- 2021-10-15
- Publication Date
- 2026-05-21
- Estimated Expiration
- 2041-10-15
AI Technical Summary
Technical Problem
Conventional nucleic acid sequencing methods using photoprotective agents suffer from high concentration issues that lead to salt precipitation, oxidation, and increased costs, affecting sequencing quality and efficiency.
Method used
The use of saponin compounds, such as notoginsenosides and ginsenosides, as photodamage protectants at very low concentrations in scanning reagents, which do not absorb UV-visible light and maintain sequencing quality during long-term storage.
Benefits of technology
Saponin compounds effectively protect template nucleotides, maintaining high data quality and preventing interference with base recognition signals, even after multiple sequencing reactions, thus enhancing nucleic acid sequencing performance.
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Smart Images
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Abstract
The present invention relates to the field of nucleic acid sequencing. In particular, the present invention relates to a scanning reagent comprising a saponin compound, a kit comprising the scanning reagent and a method for nucleic acid sequencing by using the scanning reagent.
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Citation Information
Patent Citations
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