A slice transfer assembly, a slice transfer method, and a slice transfer system

Abstract

The application discloses a slice transfer assembly, a slice transfer method and a slice transfer system. The assembly comprises a tape and a slide glass arranged oppositely, one side of the tape close to the slide glass is used for adhering a biological slice, one side of the tape away from the slide glass is provided with a hard base, one side of the hard base close to the tape is fixed with an elastic material, and the elastic material has switchable initial state and swelling state. In the initial state, the shortest distance h2 between the elastic material and the tape is greater than or equal to 0. The length of the biological slice is D, in the swelling state, the contact length between the elastic material and the tape is L1 greater than or equal to D, the contact length between the slide glass and the biological slice is L2 equal to D, and during the transfer, F 玻 >F 胶 , F 玻 represents the adsorption force of the slide glass to the biological slice, and F 胶 represents the adsorption force of the tape to the biological slice. The assembly can make the biological slice and the slide glass flatly adhere and then transfer, the biological slice is subjected to more uniform force, and the success rate and the yield of the transfer can be improved.

Description

Technical Field

[0001] This invention belongs to the field of biomedicine, and more specifically, relates to a slide transfer component, a slide transfer method, and a slide transfer system. Background Technology

[0002] In the biomedical field, it is often necessary to perform section imaging on biological tissue samples. In some applications, the tissue sample needs to be cut first, and then the sections collected and transferred to an imaging device for observation. Currently, there are generally two methods for collecting sections from flat-push cutting: one is a cyclic collection method based on a conveyor belt, and the other is a roll-to-roll collection method based on adhesive tape.

[0003] After the slides are collected, further processing such as immunohistochemical staining, micro-tissue sampling, and biochemical measurements is sometimes required. However, because chemical reagents may react with the adhesive tape substrate, some staining procedures and biochemical measurements cannot be performed directly on the tape substrate. Furthermore, the reuse of the transfer belt can contaminate the tissue slides and introduce impurities. Therefore, the common method is to transfer the tissue slides to a glass slide before performing subsequent biochemical or sampling operations. In the two existing slide collection methods, after collection, the slides are usually directly held from the transfer belt or adhesive tape with tweezers and transferred to a glass slide. This manual slide transfer method has low precision and poor consistency, is extremely prone to slide breakage, and the slides cannot be laid flat on the glass slide, ultimately affecting subsequent biochemical or sampling operations. Summary of the Invention

[0004] In view of the above-mentioned defects or improvement needs of the existing technology, the present invention provides a slide transfer component, slide transfer method and slide transfer system, the purpose of which is to solve the problem of smooth and non-destructive transfer of biological tissue slides to glass slides.

[0005] To achieve the above objectives, according to one aspect of the present invention, a slide transfer assembly is provided, comprising: an adhesive tape and a glass slide disposed opposite to each other, wherein the side of the adhesive tape near the glass slide is used to adhere the biological slide, and a rigid substrate is disposed on the side of the adhesive tape away from the glass slide; an elastic material is fixed to the side of the rigid substrate near the adhesive tape, the elastic material having a switchable initial state and an expanded state: in the initial state, the shortest distance between the elastic material and the adhesive tape is h2, h2 ≥ 0; in the expanded state, the contact length between the elastic material and the adhesive tape is L1, the contact length between the glass slide and the biological slide is L2, the length of the biological slide is D, L1 ≥ D, L2 = D, and during transfer, , This indicates the adhesion of the glass slide to the biological slice. This indicates the adhesive force of the tape on the biological slice.

[0006] Through the above technical solution, the elastic material can switch from its initial state to an expanded state, thereby pushing the adhesive tape towards the glass slide. During this switching process, the contact length L2 between the biological slide and the glass slide increases, and the contact length L1 between the elastic material and the adhesive tape also increases, until L1 ≥ D and L2 = D. At this point, the biological slide and the glass slide can be smoothly adhered together. Then, the increased adhesion of the glass slide to the biological slide transfers the biological slide from the adhesive tape to the glass slide. Furthermore, the elastic material can be further switched from its expanded state to its initial state for reuse, preparing for the next transfer.

[0007] This invention also provides a slide transfer method for transferring biological slides adhered to adhesive tape onto a glass slide, using the aforementioned slide transfer assembly, and comprising the following steps:

[0008] Gas / heat is supplied to the elastic material to switch it from its initial state to an expanded state, so that it expands in the direction of the tape to which the biological slice is attached, thereby pushing the biological slice to fit flatly against the glass slide.

[0009] Adjustment This allows the biological slice to be transferred from the tape to the glass slide.

[0010] The above technical solution involves supplying air or heat to the elastic material, causing it to expand towards the adhesive tape while remaining stationary on the side closest to the rigid substrate. After expansion, the elastic material pushes the adhesive tape closer to the glass slide until L1 ≥ D, achieving a smooth fit between the biological section and the glass slide. Then, by adjusting the adhesion force of the glass slide or adhesive tape to the biological section, the section is smoothly transferred from the tape to the slide. Because biological sections are thin and fragile, this transfer method ensures more even stress distribution, effectively preventing breakage and improving the success rate and yield of the transfer.

[0011] Preferably, adjustment The process includes: before transfer, spin-coating a UV adhesive onto the side of the slide closest to the biological slice; during transfer, irradiating the UV adhesive with a UV lamp from the side of the slide away from the biological slice to cure the UV adhesive.

[0012] Preferably, the biological section is a frozen section, adjusted This includes: raising the temperature of the glass slide above that of the frozen section.

[0013] Preferably, the biological section is a paraffin section, the tape is electrostatic tape, and the glass slide is an electrostatic glass slide. This includes: during transfer, spraying atomized droplets onto the electrostatic tape to eliminate the adhesion of the electrostatic tape to the biological slice.

[0014] Preferably, adjustment This includes: making the side of the glass slide close to the tape sticky, and the stickiness of the glass slide is higher than that of the tape.

[0015] Preferably, air is supplied to the elastic material, and the air supply amount is P. ≤ P≤ + 2.5 MPa, h1 represents the distance between the glass slide and the surface of the biological slice in the initial state, 2a, 2b, and 2c represent the triaxial lengths of the elastic material in the initial state, of which the length of the axis perpendicular to the tape is 2b, h2 represents the shortest distance between the elastic material and the tape in the initial state, n is the amount of gas, r is the molar gas constant, and t represents the temperature of the gas.

[0016] Preferably, the elastic material is heated, with the temperature of the elastic material before heating being T1 and the temperature of the elastic material after heating being T2. + ( h1 represents the distance between the glass slide and the surface of the biological slice in the initial state, 2b represents the length of the elastic material along the axis perpendicular to the tape in the initial state, h2 represents the shortest distance between the elastic material and the tape in the initial state, and α represents the coefficient of thermal volume expansion.

[0017] The present invention also proposes a slice transfer system, wherein the slice transfer system includes the above-mentioned slice transfer assembly, and further includes a take-up roller, a pressing roller and an unwinding roller, wherein the adhesive tape is movably wound on the take-up roller, the pressing roller and the unwinding roller, the adhesive tape adheres to the biological slice at the pressing roller, and the slice transfer assembly is located between the pressing roller and the take-up roller.

[0018] Preferably, the tape is an electrostatic tape, and an electrostatic generator is further provided between the unwinding roller and the pressing roller; the electrostatic generator includes a high-voltage generator head and a grounding electrode disposed opposite to the high-voltage generator head, the tape is located between the high-voltage generator head and the grounding electrode, and the electrostatic generator further includes a first translation stage for adjusting the distance between the high-voltage generator head and the tape and a second translation stage for adjusting the distance between the grounding electrode and the tape;

[0019] Alternatively, the electrostatic generator may include several friction rods, which are arranged sequentially along the forward direction of the tape.

[0020] This slide transfer system allows for the collection and transfer of biological slides by attaching an electrostatic charge to an adhesive tape and then using electrostatic adsorption to adhere the cut biological slides to the tape. The slides are then transported between the slide and the elastic material in the slide transfer assembly using take-up and untake-up rollers. Attached Figure Description

[0021] Figure 1 This is a schematic diagram before the slice is transferred;

[0022] Figure 2 This is a schematic diagram of slice transfer;

[0023] Figure 3 This is a schematic diagram of the contact between the elastic material and the tape during the transfer process;

[0024] Figure 4 This is a schematic diagram of the slice transfer system in some embodiments;

[0025] Figure 5 This is a schematic diagram of the structure of an electrostatic generator in some embodiments;

[0026] Figure 6 This is a schematic diagram of the electrostatic generator in some other embodiments;

[0027] Figure 7 This is a schematic diagram of the structure of the slice transfer system in some other embodiments.

[0028] In the diagram, 101 is the slide transfer assembly; 1 is the glass slide; 2 is the adhesive tape; 3 is the biological slide; 4 is the rigid substrate; 5 is the elastic material; 6 is the unwinding roller; 7 is the pressing roller; 8 is the rewinding roller; 9 is the electrostatic generator; 10 is the electrostatic measuring instrument; 11 is the high-voltage generator head; 12 is the grounding electrode; 13 is the first translation stage; 14 is the second translation stage; 15 is the friction rod; and 16 is the heat preservation channel. Detailed Implementation

[0029] To make the objectives, technical solutions, and advantages of this invention clearer, the invention will be further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative and not intended to limit the invention. Furthermore, the technical features involved in the various embodiments of this invention described below can be combined with each other as long as they do not conflict with each other.

[0030] like Figure 1 and Figure 2As shown, this invention proposes a slide transfer assembly, including an adhesive tape 2 and a glass slide 1 arranged opposite each other. A biological slide 3 is adhered to the side of the adhesive tape 2 near the glass slide 1, and a rigid substrate 4 is disposed on the side of the adhesive tape 2 away from the glass slide 1. An elastic material 5 is fixed to the side of the rigid substrate 4 near the adhesive tape 2. The elastic material 5 has a switchable initial state and an expanded state. In the initial state, the shortest distance between the elastic material 5 and the adhesive tape 2 is h2, where h2 ≥ 0. In the expanded state, the contact length between the elastic material 5 and the adhesive tape 2 is L1, the contact length between the glass slide 1 and the biological slide 3 is L2, the length of the biological slide is D, where L1 ≥ D, L2 = D, and during transfer... , This indicates the adhesion of slide 1 to biological section 4. This indicates the adhesion of tape 3 to biological slice 4.

[0031] Specifically, the elastic material 5 is hollow inside, initially elliptical in shape, and can be made of materials such as chlorosulfonated polyethylene or silicone rubber. The elastic material 5 can expand by supplying gas, or it can be filled with a solid or liquid and expand by heating the solid or liquid. By supplying gas / heat to the elastic material 5, causing it to expand, the tape 2 is pushed closer to the glass slide 1. When L1 ≥ D and L2 = D, the biological slide 3 is flatly adhered to the glass slide 1, and then... The biological slide 3 is transferred onto the glass slide 1. Compared with the transfer using tweezers or similar tools in existing technologies, the force applied to the biological slide 3 can be more evenly distributed across the entire slide 3, reducing the likelihood of breakage or damage. The transfer effect is optimal when the tape 2 and the glass slide 1 are parallel and the rigid substrate 4 is parallel to the tape 2.

[0032] The glass slide 1 can be held using a robotic arm or a linear motor in conjunction with a clamp. After clamping, the glass slide 1 is placed parallel to the adhesive tape 2. When expansion is performed using air supply, an air supply port and a gas supply pipe can be connected to the elastic material 5, and a pressure valve can be installed on the gas supply pipe. The air supply volume can be controlled by monitoring changes in the pressure valve. When expansion is performed using heat supply, a heating device and a thermometer can be connected to the elastic material 5 to control the heating temperature.

[0033] This invention also proposes a slice transfer method, implemented based on the above-mentioned slice transfer component, comprising the following steps:

[0034] Gas / heat is supplied to the elastic material 5 to switch the elastic material 5 from the initial state to the expanded state, so that it expands in the direction of the tape 2 with the biological slice 3 attached, and pushes the biological slice 2 to flatly adhere to the glass slide 1.

[0035] Adjustment This allows the biological slice 3 to be transferred from the tape 2 onto the glass slide 1.

[0036] During the process of smoothly attaching the biological slice 3 on the tape 2 to the glass slide 1, the glass slide 1 and the tape 2 remain parallel, while the elastic material 5 is supplied with air or heat on the side of the tape 2 away from the glass slide 1.

[0037] When expansion is achieved by supplying air, air is supplied directly through the air pipe connected to the elastic material 5, and the increase in air pressure, which is the final amount of air supplied to the elastic material 5, is monitored by a pressure valve. Specifically, the increase in air pressure by the pressure valve is P. ≤ P ≤ +2.5MPa, h1 represents the distance between the glass slide 1 and the surface of the biological slice 3 in the initial state, 2a, 2b, and 2c represent the triaxial lengths of the elastic material 5 in the initial state, of which the length perpendicular to the tape 2 is 2b, h2 represents the shortest distance between the elastic material 5 and the tape 2 in the initial state, n is the amount of gas, r is the molar gas constant, and t represents the temperature of the gas. Ideally, when P = At this point, the contact length between the elastic material 5 and the tape 2 is exactly equal to the length D of the biological slide 3. The larger the P value, the longer the contact length between the elastic material 5 and the tape 2, and the greater the force exerted by the elastic material 5 on the slide 1. Typically, the maximum pressure that the biological slide 3 can withstand per square centimeter does not exceed 250 N. Therefore, the maximum value of P is limited to... Within a range of +2.5 MPa, excessive gas supply can effectively prevent damage to the biological slide 3.

[0038] In some scenarios, when the difference between the adsorption force of the glass slide 1 on the biological slice 3 and the adsorption force of the tape 2 on the biological slice 3 is small, a larger gas supply can be selected within the above range to apply additional pressure to the biological slice 3 by the elastic material 5. Therefore, as long as a, b, c, D, h1, and h2 are determined, a gas supply can be selected within the above range according to the actual scenario to supply gas to the elastic material 5, achieving a wide-range adaptive pushing effect. Alternatively, the transfer can be observed while supplying gas within the above range. If the expected state is achieved, the gas supply can be stopped in time without repeated manual observation and adjustment.

[0039] When expansion is achieved through heating, the liquid or solid within the elastic material 5 can be directly heated. The temperature of the elastic material 5 before heating is T1 (ambient temperature), and the temperature of the elastic material 5 after heating is T2. + ( h1 represents the initial distance between the glass slide 1 and the surface of the biological slice 3; 2a, 2b, and 2c represent the triaxial lengths of the elastic material 5 in the initial state, with 2b being the length perpendicular to the tape 2; h2 represents the shortest distance between the elastic material 5 and the tape 2 in the initial state; and α represents the coefficient of thermal expansion, a constant determined by the material. This utilizes the principle of thermal expansion of some solid or liquid substances. + ( When the contact length between the elastic material 5 and the adhesive tape 2 is exactly equal to the length D of the biological slice 3, precise control of the elastic material 5 can be achieved; of course, It is not limited to this value; it can also be greater than this value. + ( ), The higher the temperature, the longer the contact length between the elastic material 5 and the tape 2, and the greater the force exerted by the elastic material 5 on the slide 1. However, excessively high temperatures can also cause irreversible damage to the biological section 3. Therefore, it is preferable to... + ( As long as b, D, h1, h2, α, and T1 are determined, an air supply volume can be selected within the above range according to the actual scenario to supply air to the elastic material 5, thereby achieving a wide-range adaptive pushing effect.

[0040] Compared with traditional manual clamping and transfer, this method clearly defines the scope of heating and gas supply, enabling automated, batch, and highly efficient transfer.

[0041] like Figure 2 and Figure 3 As shown, in the initial state, the volume of elastic material 5 is... = During transfer, the end of the elastic material 5 closest to the tape 2 is in close contact with the tape 2. Therefore, after expansion, the length of the axis of the elastic material 5 in the direction perpendicular to the tape 2 is... Let the axis of the elastic material 5 parallel to the direction of the tape 2 be the major axis. Then, when the distance from the major axis on the elastic material 5 is... A cross-section is made at the location specified in the diagram. When this cross-section equals the sample length D, the approximation function is considered complete. At this point, the cross-section of the elastic material 5 can be considered as a circle with the same radius as the minor axis of the ellipse (i.e., the axis perpendicular to the length direction of the tape 2), and its radius is R = When the cross section formed by the circle reaches D, a tight fit can be achieved. Ideally, the elastic material 5 expands uniformly during expansion, meaning the coefficients of expansion along the three axes are the same. Therefore, the volume after expansion... The pressure that the pressure valve should raise is ,Right now .

[0042] If expansion is achieved through heating, after expansion... α is the coefficient of thermal volume expansion. +( ) ,Right now, + ( ).

[0043] Let the weight of biological slide 3 be g. For biological slide 3 to be successfully transferred from tape 2 to slide 1, the following conditions must be met when slide 1 is above tape 2: When slide 1 is located below tape 2, the following conditions must be met. Biological slices are typically on the order of micrometers in thickness, and gravity is negligible. Therefore, as long as... That's all.

[0044] Specifically, There are several ways to implement this:

[0045] In some embodiments, regardless of whether the tape 2 is an electrostatic tape 2 or the type of biological section 3, UV adhesive can be spin-coated on the side of the slide 1 close to the biological section 3 before transfer. During transfer, a UV lamp is used to irradiate the UV adhesive from the side of the slide 1 away from the biological section 3 to cure the UV adhesive.

[0046] Biological section 3 can be a frozen section, a paraffin section, or a section embedded in other ways. It is cured with UV adhesive to provide a greater than [amount missing] on the slide 1. Adsorption force With the expansion and pressing of the elastic material 5, the biological slice 3 can be smoothly transferred onto the glass slide 1.

[0047] In some embodiments, the biological section 3 is a paraffin section, the adhesive tape 2 is an electrostatic tape, and the glass slide 1 is an electrostatic glass slide 1. During transfer, an atomizing nozzle is used to spray atomized droplets onto the electrostatic tape to eliminate the adhesion of the electrostatic tape to the biological section. At this time, ,and, Therefore, as long as the electrostatic glass slide 1 has electrostatic adsorption force, it can be transferred smoothly.

[0048] In some embodiments, the biological section 3 is a frozen section, and the temperature of the slide 1 is higher than that of the frozen section. The temperature difference between the slide 1 and the frozen section provides a greater... Adsorption force Specifically, before transfer, the slide 1 can be heated to 20°C~35°C using a semiconductor heating element, while the temperature of the frozen section is typically -10°C~-30°C. This significant temperature difference allows the tape 2 to press the frozen section onto the slide 1 more effectively. The frozen sections adhere firmly to the glass slide 1. Furthermore, this transfer process can be carried out within the heat-insulating channel 16 for better results. After clamping the heated glass slide 1, the glass slide 1 can be fed into the heat-insulating channel 16 through the transfer port. Then, pressure is applied to the elastic material 5 within the heat-insulating channel 16 to push the adhesive tape 2 and the biological section 3 closer to the glass slide 1, thereby completing the transfer.

[0049] In some embodiments, the side of the slide 1 closest to the adhesive tape 2 is adhesive, and the adhesiveness of the slide 1 is higher than that of the adhesive tape 2. That is, the slide is collected using the low-adhesion adhesive tape 2, and then transferred using the high-adhesion slide 1. This transfer method requires no additional operating steps, is simpler, and is suitable for different types of biological slides 3.

[0050] like Figure 4 As shown, the present invention also proposes a slice transfer system, including the aforementioned slice transfer assembly 101, and further including a take-up roller 8, a pressing roller 7, and an unwinding roller 6. The tape 2 is movably wound around the take-up roller 8, the pressing roller 7, and the unwinding roller 6. A cutting tool and a cuboid biological sample are also provided at the pressing roller 7. Through the relative movement between the cutting tool and the biological sample, and in conjunction with the pressing roller 7, the biological sample is cut layer by layer to form biological slices 3 with a certain thickness. The take-up roller 8 winds up the tape 2 during the cutting process. Therefore, the tape 2 can adhere the biological slices 3 to the pressing roller 7 at the same time as cutting. The slice transfer assembly 101 is located between the pressing roller 7 and the take-up roller 8.

[0051] In some embodiments, the tape 2 can be an electrostatic tape, and an electrostatic generator 9 is also provided between the unwinding roller 6 and the pressure roller 7. The electrostatic generator 9 is provided between the unwinding roller 6 and the pressure roller 7 to impart a static charge to the adhesive-free film, forming an electrostatic tape. This allows the biological slices 3 to be adhered during the take-up process using electrostatic adsorption. Furthermore, an electrostatic meter 10 can be provided between the electrostatic generator 9 and the pressure roller 7 to monitor the amount of static charge on the tape. Since the electrostatic adsorption force required during take-up varies depending on the tape material and the thickness of the biological slices 3, the parameters of the electrostatic generator 9 can be further adjusted by monitoring the amount of static charge on the tape 2, ensuring that the amount of static charge on the tape 2 meets the take-up requirements.

[0052] like Figure 5 As shown, the electrostatic generator 9 further includes a high voltage generating head 11 and a grounding electrode 12 disposed opposite to the high voltage generating head 11. The tape 2 is located between the high voltage generating head 11 and the grounding electrode 12. The electrostatic generator 9 also includes a first translation stage 13 for adjusting the distance between the high voltage generating head 11 and the tape 2 and a second translation stage 14 for adjusting the distance between the grounding electrode 12 and the tape 2.

[0053] Or such as Figure 6 As shown, the electrostatic generator 9 includes several friction rods 15, which are arranged sequentially along the forward direction of the tape 2.

[0054] Based on the electrostatic measuring instrument 10, the distance between the high voltage generator 11, the tape 2 and the grounding electrode 12 can be adjusted by the first translation stage 13 and the second translation stage 14, thereby adjusting the amount of static charge on the tape 2; when using another electrostatic generator 9, the distance and number of several friction rods 15 can also be adjusted according to the results of the electrostatic measuring instrument 10, thereby adjusting the amount of static charge on the tape 2.

[0055] like Figure 7 As shown, in some embodiments, the biological slice 3 can be a frozen slice. A heat-insulating channel 16 is also provided between the tableting roller 7 and the take-up roller 8. The heat-insulating channel 16 covers the forward path of the tape 2 and includes a pipe made of a high thermal conductivity material and a heat-insulating layer covering the pipe. Frozen slices are usually kept at -10 ℃ to -30 ℃. Since the slicing action is often carried out in a frozen environment such as a cryostat, if the transfer action is carried out at room temperature, a large temperature difference will occur, causing the biological slice 3 to melt and adhere firmly to the tape 2, which is not conducive to the transfer. Therefore, the heat-insulating channel 16 is set after cutting, i.e. after the tableting roller 7, to keep the biological slice 3 warm after leaving the cryostat and avoid the above situation. Furthermore, the interior of the insulation channel 16 is made of a highly thermally conductive material, resulting in high heat transfer efficiency. When the insulation channel 16 is relatively long, a semiconductor cooling device can be installed at any point inside the pipe to further cool the interior of the insulation channel 16. This cooling can be quickly conducted to the entire pipe, improving the protection of the frozen slices. Simultaneously, the pipe can be formed by splicing together multiple short pipe sections, allowing for flexible adjustment of the curvature and length of the insulation pipe according to the overall structure of the transfer system.

[0056] The slice transfer assembly 101 can be located at the outlet of the heat-insulating channel 16 or inside the heat-insulating channel 16. Preferably, the glass slide 1 is placed inside the heat-insulating channel 16 (i.e., the transfer is performed inside the heat-insulating channel 16). At this time, the elastic material 5 also needs to be located inside the heat-insulating channel 16. Therefore, in order to avoid the transfer affecting the temperature inside the heat-insulating channel 16, it is optimal to choose an expansion method using air supply. Specifically, a transfer port is opened on the heat-insulating channel 16 for the glass slide 1 to enter and exit. Outside the heat-insulating channel 16, a robotic arm or clamp is set to hold the glass slide 1. When using the clamp, a linear motor can also be used for drive, so that the glass slide 1 can be fed into the transfer port in a state parallel to the tape 2 and transferred inside the heat-insulating channel 16.

[0057] Those skilled in the art will readily understand that the above description is merely a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, and improvements made within the spirit and principles of the present invention should be included within the scope of protection of the present invention.

Claims

1. A slice transfer component, characterized in that, Comprise: The relative setting tape and glass slide, the tape is close to the side of the glass slide for sticking biological section, the tape is away from the side of the glass slide is provided with hard base, the hard base is close to the side of the tape is fixed with elastic material, the elastic material has switchable initial state and inflation state: initial state, the shortest distance between the elastic material and the tape is h2, h2≥0; inflation state, the contact length between the elastic material and the tape is L1, the contact length between the glass slide and the biological section is L2, the length of the biological section is D, L1≥D, L2=D, and transfer, F 玻 >F 胶 , F 玻 The adsorption force of the glass slide to the biological section is represented by F 胶 The adsorption force of the tape to the biological section is represented by F.

2. A slice transfer method, characterized by, The slice transfer assembly of claim 1, comprising the steps of: Supplying air / heat to the elastic material to switch the elastic material from an initial state to an expanded state to expand in the direction of the adhesive tape with the biological slice adhered to it, and push the biological slice to flatly adhere to the glass slide; Adjust F 玻 >F 胶 causing the biological section to transfer from the tape to the glass slide.

3. The slice transfer method of claim 2, wherein, Adjustment F 玻 F 胶 , comprising: before transferring, spin-coating UV glue on the side of the glass slide close to the biological section; during transferring, irradiating the UV glue from the side of the glass slide far from the biological section using a UV lamp to solidify the UV glue.

4. The slice transfer method of claim 2, wherein, The biological section is a frozen section, adjusting F 玻 >F 胶 , including: the temperature of the slide is higher than the frozen section.

5. The slice transfer method of claim 2, wherein, The biological section is a paraffin section, the adhesive tape is an electrostatic adhesive tape, the glass slide is an electrostatic glass slide, and F 玻 >F 胶 The method comprises: when transferring, spraying atomized liquid drops to the electrostatic adhesive tape to eliminate the adsorption force of the electrostatic adhesive tape on the biological section.

6. The slice transfer method of claim 2, wherein, Adjust F 玻 >F 胶 comprising: making the side of the slide close to the tape sticky, and the stickiness of the slide is higher than that of the tape.

7. The slice transfer method according to any of claims 3-6, characterized by, supplying gas to the elastic material, the amount of gas supplied being P, hi represents the distance between the slide and the surface of the biological section in the initial state, 2a, 2b, 2c represent the three axial lengths of the elastic material in the initial state, respectively, wherein the axial length perpendicular to the adhesive tape is 2b, h2 represents the shortest distance between the elastic material and the adhesive tape in the initial state, n is the amount of substance of the gas, r is the molar gas constant, and t represents the temperature of the gas.

8. The slice transfer method according to any of claims 3-6, characterized by, heating the elastic material, the temperature of the elastic material before heating being T1 and the temperature of the elastic material after heating being T2, hi represents the distance between the slide and the surface of the biological section in the initial state, 2a, 2b, 2c represent the three axial lengths of the elastic material in the initial state, respectively, wherein the axial length perpendicular to the adhesive tape is 2b, h2 represents the shortest distance between the elastic material and the adhesive tape in the initial state, and a represents the temperature-volume expansion coefficient.

9. A slice transfer system, characterized by, The slice transfer system comprises the slice transfer assembly of claim 1, and further comprises a winding roller, a pressing roller and a releasing roller, the adhesive tape is movably arranged on the winding roller, the pressing roller and the releasing roller, the biological slice is adhered to the adhesive tape at the pressing roller, and the slice transfer assembly is located between the pressing roller and the winding roller.

10. The slice transfer system of claim 9, wherein, The adhesive tape is an electrostatic adhesive tape, and an electrostatic generator is further arranged between the releasing roller and the pressing roller. The electrostatic generator comprises a high-voltage generating head and a grounding electrode arranged opposite to the high-voltage generating head, the adhesive tape is located between the high-voltage generating head and the grounding electrode, the electrostatic generator further comprises a first translation stage for adjusting the distance between the high-voltage generating head and the adhesive tape, and a second translation stage for adjusting the distance between the grounding electrode and the adhesive tape. Or the electrostatic generator comprises a plurality of friction rods, and the adhesive tape is sequentially arranged on the friction rods.

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