A method, apparatus and electronic device for detecting abnormalities in sample needle aspiration

Abstract

This invention provides a method, apparatus, and electronic device for detecting abnormal sample needle aspiration. The method includes: acquiring a sensor self-test pressure value; determining whether the sample dispensing system is in a normal or abnormal state based on the self-test pressure value; performing a liquid aspiration operation based on the normal state of the sample dispensing system; acquiring a sample needle aspiration pressure waveform; and determining whether the sample needle is in a normal state, an empty aspiration state, or a blocked state based on the aspiration pressure waveform; and acquiring a sample needle cleaning pressure waveform; and determining whether the sample needle is in a normal filling cleaning state, an abnormal non-filling cleaning state, or a blocked cleaning state based on the cleaning pressure waveform. The sample needle aspiration abnormality detection method provided by this invention can achieve real-time detection of multiple abnormal states, ensuring the accuracy of test results.

Description

Technical Field

[0001] This invention relates to the field of in vitro diagnostic equipment testing, specifically to a method, apparatus, and electronic device for detecting abnormalities in sample needle aspiration. Background Technology

[0002] In the in vitro diagnostics industry, the samples encountered by diagnostic equipment may contain various impurities, which can cause abnormalities such as blockage of the sample needle or the intake of air when drawing the sample. This directly leads to inaccurate sample volume and thus unreliable test results.

[0003] Therefore, real-time monitoring of the sample dispensing circuit and the status of the sample needle is particularly important. Existing detection mainly focuses on the fixed value judgment of the pressure inside the pipeline, which is relatively simple in function and has weak stability. It cannot completely distinguish the following abnormal states that are prone to occur: the presence of bubbles or air bubbles on the sample surface, or false detection of liquid level causing the sample dispensing needle to not enter the actual liquid surface for sample aspiration, resulting in empty aspiration; the sample needle being immersed too deeply, the presence of impurities in the sample, insufficient sample, aspiration of separating gel or clots, or blockage caused by pressing the bottom of the cup; the presence of air in the inner wall pipeline due to insufficient cleaning fluid during the filling and cleaning of the inner wall pipeline of the sample dispensing system; and blockage of the inner wall pipeline during the cleaning of the sample dispensing system.

[0004] In summary, the existing technology does not comprehensively detect abnormal states in sample needle aspiration, thus affecting the accuracy of test results. Summary of the Invention

[0005] In view of this, it is necessary to provide a method, device and electronic device for detecting abnormal sample needle aspiration, so as to solve the technical problem that the detection of abnormal sample needle aspiration is not comprehensive enough in the existing technology, resulting in low accuracy of test results.

[0006] To address the aforementioned technical problems, this invention provides a method for detecting abnormal sample needle aspiration, applied to a sample dispensing system, comprising:

[0007] The sensor self-test pressure value is obtained, and the self-test pressure value is compared with the preset self-test pressure range to determine whether the sampling system is in a normal or abnormal state.

[0008] The sample dispensing system is used to perform liquid aspiration operation under normal conditions. The liquid aspiration pressure waveform of the sample needle is obtained. The empty aspiration indicator pressure value is determined based on the liquid aspiration pressure waveform. The empty aspiration indicator pressure value is compared with the preset empty aspiration judgment pressure range to determine whether the sample needle is in normal condition or empty aspiration condition.

[0009] The pressure value for identifying the blocked needle is determined based on the liquid aspiration pressure waveform. The pressure value for identifying the blocked needle is then compared with the preset pressure range for determining blocked needles to determine whether the sample needle is in a normal state or a blocked state.

[0010] The cleaning operation is performed based on the sample dispensing system under normal conditions. The sample needle cleaning pressure waveform is obtained. The cleaning filling indicator pressure array is determined based on the cleaning pressure waveform. The filling indicator pressure array is compared with the preset filling judgment pressure range to determine whether the sample needle is in a normal filling cleaning state or an abnormal non-fill cleaning state.

[0011] The cleaning pressure waveform is used to determine the cleaning needle blockage identification pressure array. The cleaning needle blockage identification pressure array is compared with the preset cleaning needle blockage judgment pressure range to determine whether the sample needle is in normal cleaning state or needle blockage cleaning state.

[0012] In some possible implementations, the liquid aspiration operation includes: sample aspiration operation, reagent aspiration operation, and diluent aspiration operation;

[0013] The volume of liquid absorbed in the sample aspiration operation is less than a first preset volume value; the volume of liquid absorbed in the reagent aspiration operation is greater than or equal to the first preset volume value and less than a second preset volume value; and the volume of liquid absorbed in the diluent aspiration operation is greater than or equal to the second preset volume value.

[0014] In some possible implementations, the step of acquiring the sample needle aspiration pressure waveform, determining the empty aspiration indicator pressure value based on the aspiration pressure waveform, and comparing the empty aspiration indicator pressure value with a preset empty aspiration judgment pressure range to determine the normal working state or empty aspiration state of the sample needle includes:

[0015] Acquire the sample needle aspiration pressure waveform, which includes the sample aspiration pressure waveform, the reagent aspiration pressure waveform, and the diluent aspiration pressure waveform;

[0016] The sample aspiration initiation pressure value and sample aspiration judgment pressure value are obtained based on the sample aspiration pressure waveform. The sample empty aspiration indicator pressure value is obtained by subtracting the sample aspiration initiation pressure value from the sample aspiration judgment pressure value. The sample empty aspiration indicator pressure value is compared with the preset sample empty aspiration judgment pressure range to determine the normal working state or empty aspiration state of the sample needle during sample aspiration.

[0017] The reagent aspiration initiation pressure value and reagent aspiration judgment pressure value are obtained based on the reagent aspiration pressure waveform. The reagent empty aspiration indicator pressure value is obtained by subtracting the reagent aspiration initiation pressure value from the reagent aspiration judgment pressure value. The reagent empty aspiration indicator pressure value is compared with the preset reagent empty aspiration judgment pressure range to determine whether the sample needle is in normal working state or empty aspiration state during reagent aspiration.

[0018] The starting pressure value and the judgment pressure value of the diluent aspiration are obtained according to the diluent aspiration pressure waveform. The diluent empty aspiration indicator pressure value is obtained by subtracting the starting pressure value of the diluent aspiration from the judgment pressure value of the diluent aspiration. The diluent empty aspiration indicator pressure value is compared with the preset diluent empty aspiration judgment pressure range to determine the normal working state or empty aspiration state of the sample needle when aspirating the diluent.

[0019] The aspiration pressure waveform includes the sample aspiration pressure waveform, the reagent aspiration pressure waveform, and the diluent aspiration pressure waveform; the empty aspiration indicator pressure value includes the sample empty aspiration indicator pressure value, the reagent empty aspiration indicator pressure value, and the diluent empty aspiration indicator pressure value; and the preset empty aspiration judgment pressure range includes the preset sample empty aspiration judgment pressure range, the preset reagent empty aspiration judgment pressure range, and the preset diluent empty aspiration judgment pressure range.

[0020] In some possible implementations, determining the needle plugging indicator pressure value based on the liquid suction pressure waveform includes:

[0021] The suction initiation pressure value and the suction flow rate peak pressure value are obtained based on the suction pressure waveform. The suction initiation pressure value is obtained by subtracting the suction flow rate peak pressure value from the suction flow rate peak pressure value.

[0022] In some possible implementations, the preset needle blockage detection pressure range is the same for the sample aspiration operation, reagent aspiration operation, and diluent aspiration operation.

[0023] In some possible implementations, determining the cleaning filling indicator pressure array based on the cleaning pressure waveform includes:

[0024] The cleaning start pressure value, cleaning maximum value, and cleaning minimum value are obtained based on the cleaning pressure waveform. The cleaning filling indicator pressure array is obtained by subtracting the cleaning start pressure value from the cleaning maximum value and the cleaning minimum value.

[0025] In some possible implementations, determining the cleaning needle plug identifier pressure array based on the cleaning pressure waveform includes:

[0026] The cleaning start pressure value, cleaning maximum pressure value, and cleaning end pressure value are obtained based on the cleaning pressure waveform. The cleaning start pressure value is then subtracted from the cleaning maximum pressure value and the cleaning end pressure value to obtain the cleaning plug needle identification pressure array.

[0027] On the other hand, the present invention also provides a sample needle aspiration abnormality detection device, applied to a sample dispensing system, comprising:

[0028] The self-test module is used to obtain the sensor self-test pressure value, and compare the self-test pressure value with the preset self-test pressure range to determine whether the sampling system is in a normal or abnormal state.

[0029] The liquid aspiration empty aspiration detection module is used to perform liquid aspiration operation based on the sample addition system under normal conditions, acquire the liquid aspiration pressure waveform of the sample needle, determine the empty aspiration indicator pressure value based on the liquid aspiration pressure waveform, and compare the empty aspiration indicator pressure value with the preset empty aspiration judgment pressure range to determine whether the sample needle is in normal condition or empty aspiration state.

[0030] The liquid aspiration needle blockage detection module is used to determine the needle blockage identification pressure value based on the liquid aspiration pressure waveform, and to compare the needle blockage identification pressure value with the preset needle blockage judgment pressure range to determine whether the sample needle is in a normal state or a blocked state.

[0031] The cleaning and filling detection module is used to perform cleaning operations based on the sample dispensing system under normal conditions, obtain the sample needle cleaning pressure waveform, determine the cleaning and filling indicator pressure array based on the cleaning pressure waveform, and compare the filling indicator pressure array with a preset filling judgment pressure interval group to determine whether the sample needle is in a normal filling cleaning state or an abnormal non-filling cleaning state.

[0032] The cleaning needle blockage detection module is used to determine the cleaning needle blockage identification pressure array based on the cleaning pressure waveform, and to compare the cleaning needle blockage identification pressure array with the preset cleaning needle blockage judgment pressure range to determine whether the sample needle is in a normal cleaning state or a blocked needle cleaning state.

[0033] In some possible implementations, the liquid aspiration empty aspiration detection module includes a sample empty aspiration detection submodule, a reagent empty aspiration detection submodule, and a diluent empty aspiration detection submodule;

[0034] The sample aspiration empty detection submodule is used to acquire the sample aspiration pressure waveform, acquire the sample aspiration starting pressure value and the sample aspiration judgment pressure value based on the sample aspiration pressure waveform, subtract the sample aspiration starting pressure value from the sample aspiration judgment pressure value to obtain the sample empty aspiration identification pressure value, and compare the sample empty aspiration identification pressure value with the preset sample empty aspiration judgment pressure range to determine the normal working state or empty aspiration state of the sample needle during sample aspiration.

[0035] The reagent aspiration detection submodule is used to acquire the reagent aspiration pressure waveform, acquire the reagent aspiration initiation pressure value and the reagent aspiration judgment pressure value based on the reagent aspiration pressure waveform, subtract the reagent aspiration initiation pressure value from the reagent aspiration judgment pressure value to obtain the reagent aspiration aspiration identification pressure value, and compare the reagent aspiration aspiration identification pressure value with the preset reagent aspiration aspiration judgment pressure range to determine whether the sample needle is in normal working state or aspiration aspiration state during reagent aspiration.

[0036] The diluent aspiration detection submodule is used to acquire the diluent aspiration pressure waveform, acquire the diluent aspiration start pressure value and the diluent aspiration judgment pressure value based on the diluent aspiration pressure waveform, subtract the diluent aspiration start pressure value from the diluent aspiration judgment pressure value to obtain the diluent aspiration aspiration indicator pressure value, and compare the diluent aspiration aspiration indicator pressure value with the preset diluent aspiration aspiration judgment pressure range to determine whether the sample needle is in normal working state or aspiration aspiration state during diluent aspiration.

[0037] Finally, the present invention also provides an electronic device, including a memory, a processor, and a computer program stored in the memory and executable on the processor. When the processor executes the program, it implements the sample needle aspiration anomaly detection method described in the above implementation.

[0038] The beneficial effects of the above embodiments are as follows: The sample needle aspiration abnormality detection method provided by the present invention realizes the detection of the entire sample dispensing system status by analyzing the pressure in the tubing under different operations in real time. It can detect empty aspiration during sample aspiration, reagent aspiration, and diluent aspiration in actual application, as well as liquid aspiration needle blockage detection, sample needle cleaning tubing filling status detection, sample needle cleaning tubing blockage status detection, and sensor status self-test, realizing real-time detection of multiple abnormal states to ensure the accuracy of test results. Attached Figure Description

[0039] To more clearly illustrate the technical solutions in the embodiments of the present invention, the accompanying drawings used in the description of the embodiments will be briefly introduced below. Obviously, the accompanying drawings described below are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0040] Figure 1 This is a flowchart illustrating an embodiment of the sample needle aspiration abnormality detection method provided by the present invention;

[0041] Figure 2 A schematic diagram of an embodiment of the liquid absorption pressure waveform provided by the present invention;

[0042] Figure 3 A schematic diagram of an embodiment of the cleaning pressure waveform provided by the present invention;

[0043] Figure 4 This is a schematic diagram of an embodiment of the sample addition system provided by the present invention;

[0044] Figure 5 A schematic diagram of a sample needle aspiration abnormality detection device according to an embodiment of the present invention;

[0045] Figure 6 A schematic diagram of the structure of an embodiment of the electronic device provided by the present invention. Detailed Implementation

[0046] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only a part of the embodiments of the present invention, and not all of them. All other embodiments obtained by those skilled in the art based on the embodiments of the present invention without creative effort are within the scope of protection of the present invention.

[0047] It should be understood that the illustrative drawings are not drawn to scale. The flowcharts used in this invention illustrate operations implemented according to some embodiments of the invention. It should be understood that the operations in the flowcharts may not be implemented in sequence, and steps without logical contextual relationships may be reversed or performed simultaneously. Furthermore, those skilled in the art, guided by the content of this invention, may add one or more other operations to the flowcharts, or remove one or more operations from the flowcharts.

[0048] Some of the block diagrams shown in the accompanying drawings are functional entities and do not necessarily correspond to physically or logically independent entities. These functional entities can be implemented in software, in one or more hardware modules or integrated circuits, or in different network and / or processor systems and / or microcontroller systems.

[0049] In this document, the term "embodiment" means that a particular feature, structure, or characteristic described in connection with an embodiment may be included in at least one embodiment of the invention. The appearance of this phrase in various places throughout the specification does not necessarily refer to the same embodiment, nor is it a separate or alternative embodiment mutually exclusive with other embodiments. It will be explicitly and implicitly understood by those skilled in the art that the embodiments described herein can be combined with other embodiments.

[0050] The specific embodiments are described in detail below. It should be noted that the order of the following descriptions of the embodiments is not intended to limit the preferred order of the embodiments.

[0051] This invention provides a method, apparatus, and electronic device for detecting abnormalities in sample needle aspiration.

[0052] like Figure 1 As shown, Figure 1 This is a flowchart illustrating an embodiment of the sample needle aspiration anomaly detection method provided by the present invention. The sample needle aspiration anomaly detection method includes:

[0053] S101. Obtain the sensor self-test pressure value, and compare the self-test pressure value with the preset self-test pressure range to determine whether the sampling system is in a normal or abnormal state.

[0054] S102. Perform liquid aspiration operation based on the normal state of the sample addition system, obtain the liquid aspiration pressure waveform of the sample needle, determine the empty aspiration indicator pressure value according to the liquid aspiration pressure waveform, and compare the empty aspiration indicator pressure value with the preset empty aspiration judgment pressure range to determine whether the sample needle is in a normal state or an empty aspiration state.

[0055] S103. Determine the needle blockage indicator pressure value based on the liquid aspiration pressure waveform, and compare the needle blockage indicator pressure value with the preset needle blockage judgment pressure range to determine whether the sample needle is in a normal state or a blocked state.

[0056] S104. Perform a cleaning operation based on the sample dispensing system under normal conditions, obtain the sample needle cleaning pressure waveform, determine the cleaning filling indicator pressure array based on the cleaning pressure waveform, and compare the filling indicator pressure array with the preset filling judgment pressure range to determine whether the sample needle is in a normal filling cleaning state or an abnormal non-filling cleaning state.

[0057] S105. Determine the cleaning needle blockage identification pressure array based on the cleaning pressure waveform, and compare the cleaning needle blockage identification pressure array with the preset cleaning needle blockage judgment pressure range to determine whether the sample needle is in normal cleaning state or needle blockage cleaning state.

[0058] Compared with existing technologies, the present invention provides a sample needle aspiration anomaly detection method that, by analyzing the pressure in the tubing under different operating conditions in real time, realizes the detection of the status of the entire sample dispensing system. It can detect liquid aspiration emptying, liquid aspiration needle blockage, sample needle cleaning tubing filling status, sample needle cleaning tubing blockage status, and sensor status self-check during actual application, realizing the real-time detection of multiple abnormal states to ensure the accuracy of test results.

[0059] It should be noted that the sample needle aspiration anomaly detection method provided by this invention compares the judgment value with the constructed judgment logic table during anomaly detection to obtain the normal or abnormal judgment result of the sample needle. The constructed judgment logic table is shown in Table 1:

[0060] Table 1 Judgment Logic Table

[0061]

[0062] Optionally, in a specific embodiment of the present invention, in step S101, during the sensor status self-test process, the self-test pressure value Ps of the instrument initialization state is obtained when the power is turned on (the pressure value within a few milliseconds is taken when no instruction is received after power-on), and compared with the preset self-test pressure range. When D0-70000≤Ps≤D0, the sampling system self-test is determined to be normal. When Ps>D0 or Ps<D0-70000, the sampling system self-test is determined to be abnormal. D0 is the preset self-test judgment parameter (obtained from actual data statistics) [unit: Pa].

[0063] Furthermore, in some embodiments of the present invention, in step S102, the liquid aspiration operation includes: sample aspiration operation, reagent aspiration operation, and diluent aspiration operation;

[0064] The volume of liquid absorbed in the sample aspiration operation is less than a first preset volume value; the volume of liquid absorbed in the reagent aspiration operation is greater than or equal to the first preset volume value and less than a second preset volume value; and the volume of liquid absorbed in the diluent aspiration operation is greater than or equal to the second preset volume value.

[0065] Acquire the sample needle aspiration pressure waveform, which includes the sample aspiration pressure waveform, the reagent aspiration pressure waveform, and the diluent aspiration pressure waveform;

[0066] The sample aspiration initiation pressure value and sample aspiration judgment pressure value are obtained based on the sample aspiration pressure waveform. The sample empty aspiration indicator pressure value is obtained by subtracting the sample aspiration initiation pressure value from the sample aspiration judgment pressure value. The sample empty aspiration indicator pressure value is compared with the preset sample empty aspiration judgment pressure range to determine the normal working state or empty aspiration state of the sample needle during sample aspiration.

[0067] The reagent aspiration initiation pressure value and reagent aspiration judgment pressure value are obtained based on the reagent aspiration pressure waveform. The reagent empty aspiration indicator pressure value is obtained by subtracting the reagent aspiration initiation pressure value from the reagent aspiration judgment pressure value. The reagent empty aspiration indicator pressure value is compared with the preset reagent empty aspiration judgment pressure range to determine whether the sample needle is in normal working state or empty aspiration state during reagent aspiration.

[0068] The starting pressure value and the judgment pressure value of the diluent aspiration are obtained according to the diluent aspiration pressure waveform. The diluent empty aspiration indicator pressure value is obtained by subtracting the starting pressure value of the diluent aspiration from the judgment pressure value of the diluent aspiration. The diluent empty aspiration indicator pressure value is compared with the preset diluent empty aspiration judgment pressure range to determine the normal working state or empty aspiration state of the sample needle when aspirating the diluent.

[0069] The aspiration pressure waveform includes the sample aspiration pressure waveform, the reagent aspiration pressure waveform, and the diluent aspiration pressure waveform; the empty aspiration indicator pressure value includes the sample empty aspiration indicator pressure value, the reagent empty aspiration indicator pressure value, and the diluent empty aspiration indicator pressure value; and the preset empty aspiration judgment pressure range includes the preset sample empty aspiration judgment pressure range, the preset reagent empty aspiration judgment pressure range, and the preset diluent empty aspiration judgment pressure range.

[0070] In specific embodiments of the present invention, such as Figure 2 As shown, Figure 2 This is a schematic diagram of an embodiment of the liquid absorption pressure waveform provided by the present invention.

[0071] It should be noted that, since the waveforms obtained from sample aspiration, reagent aspiration, and diluent aspiration are basically the same during the liquid aspiration process, the difference lies in the volume of liquid aspirated. This leads to different empty aspiration indicator pressure values ​​and pressure ranges for judging normal aspiration, but the interval range of the corresponding pressure ranges for judging empty aspiration is the same, i.e., they have the same abnormal empty aspiration judgment pressure range. This embodiment of the invention uses the common pressure waveform during the liquid aspiration process as an example for illustration. Figure 2 In the formula, P0 is the initial pressure value of liquid aspiration (which can represent the initial pressure value of sample aspiration, reagent aspiration, and diluent aspiration), P1 is the pressure value at the peak point of liquid aspiration flow rate, P2 is the sample aspiration judgment pressure value, P3 is the reagent aspiration judgment pressure value, and P4 is the diluent aspiration judgment pressure value. The first preset volume value is 55uL, and the second preset volume value is 150uL.

[0072] The sample aspiration empty aspiration detection involves capturing the pressure waveform during the sample aspiration and dissipation process. Based on the initial aspiration pressure value P0 (calculated from the pressure value between the sample needle immersing in the liquid surface and the start of the aspiration process) and the sample aspiration judgment pressure value P2 (the pressure value after the aspiration process is completed when the aspiration volume is less than a certain value), the empty aspiration indicator pressure value Pb (obtained by subtracting P0 from P2) is determined. Pb is then compared with the preset empty aspiration judgment pressure range in the logic table (derived from actual data statistics), and the result is determined as either empty aspiration or normal aspiration.

[0073] The reagent aspiration empty aspiration detection involves capturing the pressure waveform during the aspiration and dissipation of the reagent sample. Based on the initial aspiration pressure value P0 and the reagent aspiration judgment pressure value P3, the empty aspiration indicator pressure value Pc is determined (by subtracting P0 from P3). Pc is then compared with the preset reagent empty aspiration judgment pressure range in the logic table, and the judgment result is either empty aspiration or normal.

[0074] The diluent aspiration empty aspiration detection involves capturing the pressure waveform during the aspiration and dissipation of the reagent sample against the diluent. Based on the initial aspiration pressure value P0 and the diluent aspiration judgment pressure value P4, the diluent empty aspiration indicator pressure value Pd (obtained by subtracting P0 from P4) is determined. Pd is then compared with the preset diluent empty aspiration judgment pressure range in the logic table, and the judgment result is either empty aspiration or normal.

[0075] Furthermore, in some embodiments of the present invention, step S103, determining the needle plugging indicator pressure value based on the liquid suction pressure waveform, includes:

[0076] The suction initiation pressure value and the suction flow rate peak pressure value are obtained based on the suction pressure waveform. The suction initiation pressure value is obtained by subtracting the suction flow rate peak pressure value from the suction flow rate peak pressure value.

[0077] It should be noted that the preset needle blockage detection pressure range is the same for the sample aspiration operation, reagent aspiration operation, and diluent aspiration operation.

[0078] In a specific embodiment of the present invention, when the reagent sample is aspirated or expelled against the reagent / sample / diluent, the pressure waveform during this period is collected. Based on the initial aspiration pressure value P0 and the peak aspiration flow rate pressure value P1 (the point of maximum aspiration speed), the needle blockage identification pressure value Pe (obtained by subtracting P0 from P1) is determined. Pe is compared with the preset needle blockage judgment pressure range in the logic table, and the judgment result is needle blockage or normal.

[0079] Furthermore, in some embodiments of the present invention, step S104, determining the cleaning filling indicator pressure array based on the cleaning pressure waveform, includes:

[0080] The cleaning start pressure value, cleaning maximum value, and cleaning minimum value are obtained based on the cleaning pressure waveform. The cleaning filling indicator pressure array is obtained by subtracting the cleaning start pressure value from the cleaning maximum value and the cleaning minimum value.

[0081] In specific embodiments of the present invention, such as Figure 3 As shown, Figure 3 This is a schematic diagram of an embodiment of the cleaning pressure waveform provided by the present invention.

[0082] When the reagent sample needle enters the cleaning tank for cleaning, the pressure waveform during cleaning is collected, such as... Figure 3As shown, based on the cleaning start pressure value P5 (calculated from the pressure value between the sample needle entering the cleaning tank and the pump valve starting), the cleaning maximum pressure value P6 (the maximum value between a few milliseconds after the pump starts and a few milliseconds before the pump ends), and the cleaning minimum pressure value P7 (the minimum value between a few milliseconds after the pump starts and a few milliseconds before the pump ends), the cleaning filling indicator pressure array B1 (obtained by subtracting P5 from P6 and P7) is obtained after processing. After comparing it with the data in the preset filling judgment pressure range Pf in the logic table (obtained from actual data statistics), the pipeline state during sample needle cleaning is determined to be either normal filling cleaning state or abnormal non-filling cleaning state (air enters the liquid path during sample needle cleaning).

[0083] Furthermore, in some embodiments of the present invention, step S105, determining the cleaning needle identification pressure array based on the cleaning pressure waveform, includes:

[0084] The cleaning start pressure value, cleaning maximum pressure value, and cleaning end pressure value are obtained based on the cleaning pressure waveform. The cleaning start pressure value is then subtracted from the cleaning maximum pressure value and the cleaning end pressure value to obtain the cleaning plug needle identification pressure array.

[0085] In a specific embodiment of the present invention, when the reagent sample needle enters the cleaning tank for cleaning, the pressure waveform during cleaning is collected. Please refer to [the relevant documentation]. Figure 3 Based on the cleaning start pressure value P5, the cleaning maximum pressure value P6 (the maximum value in the interval from a few milliseconds after pump start to a few milliseconds before pump end), and the cleaning end pressure value P8 (the maximum pressure in the interval from a few milliseconds after pump shut-off), the cleaning needle blockage identification pressure array B2 (obtained by subtracting P5 from P6 and P8) is obtained after processing. After comparing it with the data in the preset cleaning needle blockage judgment pressure interval Pg in the logic table (obtained from actual data statistics), the pipeline status during sample needle cleaning is determined as either normal cleaning status or needle blockage cleaning status.

[0086] In a specific embodiment of the present invention, the present invention provides a method for detecting abnormal sample needle aspiration, used in a sample dispensing system, such as... Figure 4 As shown, Figure 4This is a schematic diagram of an embodiment of the sample dispensing system provided by the present invention. During the cleaning process, when the sample dispensing system 400 receives a cleaning command, the second pressure pump 407 starts to pump the cleaning solution into the cleaning tank 406, the first pressure pump 405 starts, the pump valve 404 opens, the needle blockage detection plate 403 starts to collect pressure signals during the cleaning process, and the plunger pump 402 resets. During the liquid aspiration process, the plunger pump 402 controls the sample needle 401 to aspirate liquid until the aspiration is completed. The needle blockage detection plate 403 collects the pressure signals during the aspiration process and detects and judges the abnormal state of the sample needle based on the pressure signals. The abnormal detection and judgment method of sample needle aspiration abnormality detection in the above embodiment is used for abnormality detection, which will not be described in detail here.

[0087] This invention provides a method for detecting abnormal sample needle aspiration. By analyzing the pressure within the tubing under different operating conditions in real time, it enables the detection of the entire sample dispensing system's status. It can detect empty aspiration during sample, reagent, and diluent aspiration in practical applications, as well as needle blockage detection, sample needle cleaning tubing filling status detection, sample needle cleaning tubing blockage status detection, and sensor status self-check. This achieves real-time detection of multiple abnormal states, ensuring the accuracy of test results. While it has relatively low requirements for sensor accuracy, it is highly time-dependent and applicable to pressure scenarios with a maximum tubing pressure of less than 500 kPa. It can identify empty aspiration and blocked needles during sample dispensing from 10 μL to 200 μL of liquid at ambient temperatures of 10℃-25℃, and effectively determine various states during the cleaning of the sample dispensing system's inner wall.

[0088] This invention provides a method for detecting abnormalities in sample needle aspiration. By performing hardware filtering on the original waveform, the smoothness of the curve is increased. The implementation uses a total of 3kb of resources, with data processing using less than 1kb, resulting in low resource consumption. This method can be used in pressure scenarios below 500kPa absolute pressure, with low accuracy requirements and strong timing coupling. It achieves the identification of empty aspiration of liquids with volumes ranging from 10-200 microliters at temperatures below 3000m and altitudes between 10℃ and 30℃; it also achieves the identification of blocked needles during liquid aspiration at temperatures below 3000m and altitudes between 10℃ and 30℃; it identifies the presence of air sections in the pipeline during liquid flushing at temperatures below 3000m and altitudes between 10℃ and 30℃; and it identifies whether the pipeline is blocked during liquid flushing at temperatures below 30℃ and altitudes between 30℃ and 30℃.

[0089] To better implement the sample needle aspiration anomaly detection method in this embodiment of the invention, based on the sample needle aspiration anomaly detection method, this embodiment of the invention also provides a sample needle aspiration anomaly detection device, such as... Figure 5 As shown, the sample needle aspiration abnormality detection device 500 includes:

[0090] The self-test module 501 is used to acquire the sensor self-test pressure value and compare the self-test pressure value with a preset self-test pressure range to determine whether the sampling system is in a normal or abnormal state.

[0091] The liquid aspiration empty aspiration detection module 502 is used to perform liquid aspiration operation based on the sample addition system under normal conditions, acquire the liquid aspiration pressure waveform of the sample needle, determine the empty aspiration indicator pressure value based on the liquid aspiration pressure waveform, and compare the empty aspiration indicator pressure value with the preset empty aspiration judgment pressure range to determine whether the sample needle is in normal condition or empty aspiration state.

[0092] The liquid aspiration needle blockage detection module 503 is used to determine the needle blockage identification pressure value based on the liquid aspiration pressure waveform, and to determine whether the sample needle is in a normal state or a blocked state by comparing the needle blockage identification pressure value with a preset needle blockage judgment pressure range.

[0093] The cleaning and filling detection module 504 is used to perform cleaning operations based on the sample dispensing system under normal conditions, acquire the sample needle cleaning pressure waveform, determine the cleaning and filling indicator pressure array based on the cleaning pressure waveform, and compare the filling indicator pressure array with a preset filling judgment pressure interval group to determine whether the sample needle is in a normal filling cleaning state or an abnormal non-filling cleaning state.

[0094] The cleaning needle blockage detection module 505 is used to determine the cleaning needle blockage identification pressure array based on the cleaning pressure waveform, and to determine whether the sample needle is in normal cleaning state or needle blockage cleaning state by comparing the cleaning needle blockage identification pressure array with the preset cleaning needle blockage judgment pressure range.

[0095] The liquid aspiration empty aspiration detection module includes a sample empty aspiration detection submodule 5021, a reagent empty aspiration detection submodule 5022, and a diluent empty aspiration detection submodule 5023;

[0096] The sample aspiration detection submodule 5021 is used to acquire the sample aspiration pressure waveform, acquire the sample aspiration start pressure value and the sample aspiration judgment pressure value based on the sample aspiration pressure waveform, subtract the sample aspiration start pressure value from the sample aspiration judgment pressure value to obtain the sample aspiration aspiration indicator pressure value, and compare the sample aspiration aspiration indicator pressure value with the preset sample aspiration judgment pressure range to determine the normal working state or aspiration aspiration state of the sample needle during sample aspiration.

[0097] The reagent aspiration detection submodule 5022 is used to acquire the reagent aspiration pressure waveform, acquire the reagent aspiration initiation pressure value and the reagent aspiration judgment pressure value based on the reagent aspiration pressure waveform, subtract the reagent aspiration initiation pressure value from the reagent aspiration judgment pressure value to obtain the reagent aspiration aspiration identification pressure value, and compare the reagent aspiration aspiration identification pressure value with the preset reagent aspiration judgment pressure range to determine the normal working state or aspiration aspiration state of the sample needle during reagent aspiration.

[0098] The diluent aspiration detection submodule 5023 is used to acquire the diluent aspiration pressure waveform, acquire the diluent aspiration start pressure value and the diluent aspiration judgment pressure value based on the diluent aspiration pressure waveform, subtract the diluent aspiration start pressure value from the diluent aspiration judgment pressure value to obtain the diluent aspiration aspiration indicator pressure value, and compare the diluent aspiration aspiration indicator pressure value with the preset diluent aspiration aspiration judgment pressure range to determine whether the sample needle is in normal working state or aspiration aspiration state during diluent aspiration.

[0099] The sample needle aspiration abnormality detection device 500 provided in the above embodiments can realize the technical solutions described in the above sample needle aspiration abnormality detection method embodiments. The specific implementation principles of each module or unit can be found in the corresponding content in the above sample needle aspiration abnormality detection method embodiments, and will not be repeated here.

[0100] like Figure 6 As shown, the present invention also provides an electronic device 600. The electronic device 600 includes a processor 601, a memory 602, and a display 603. Figure 3 Only some components of the electronic device 600 are shown, but it should be understood that it is not required to implement all the components shown, and more or fewer components may be implemented instead.

[0101] In some embodiments, processor 601 may be a central processing unit (CPU), microprocessor, or other data processing chip, used to run program code stored in memory 602 or process data, such as the sample needle aspiration anomaly detection program in this invention.

[0102] In some embodiments, processor 601 may be a single server or a group of servers. The server group may be centralized or distributed. In some embodiments, processor 601 may be local or remote. In some embodiments, processor 601 may be implemented on a cloud platform. In one embodiment, the cloud platform may include a private cloud, public cloud, hybrid cloud, community cloud, distributed cloud, intranet, multi-cloud, etc., or any combination thereof.

[0103] In some embodiments, memory 602 may be an internal storage unit of electronic device 600, such as a hard disk or memory of electronic device 600. In other embodiments, memory 602 may also be an external storage device of electronic device 600, such as a plug-in hard disk, smart media card (SMC), secure digital (SD) card, flash card, etc. equipped on electronic device 600.

[0104] Furthermore, the memory 602 may include both internal storage units of the electronic device 600 and external storage devices. The memory 602 is used to store application software and various types of data installed on the electronic device 600.

[0105] In some embodiments, display 603 may be an LED display, a liquid crystal display, a touch-sensitive liquid crystal display, or an OLED (Organic Light-Emitting Diode) touchscreen. Display 603 is used to display information from electronic device 600 and to display a visual user interface. Components 601-603 of electronic device 600 communicate with each other via a system bus.

[0106] In one embodiment, when the processor 601 executes the sample needle aspiration anomaly detection program in the memory 602, the following steps can be implemented:

[0107] The sensor self-test pressure value is obtained, and the self-test pressure value is compared with the preset self-test pressure range to determine whether the sampling system is in a normal or abnormal state.

[0108] The sample dispensing system is used to perform liquid aspiration operation under normal conditions. The liquid aspiration pressure waveform of the sample needle is obtained. The empty aspiration indicator pressure value is determined based on the liquid aspiration pressure waveform. The empty aspiration indicator pressure value is compared with the preset empty aspiration judgment pressure range to determine whether the sample needle is in normal condition or empty aspiration condition.

[0109] The pressure value for identifying the blocked needle is determined based on the liquid aspiration pressure waveform. The pressure value for identifying the blocked needle is then compared with the preset pressure range for determining blocked needles to determine whether the sample needle is in a normal state or a blocked state.

[0110] The cleaning operation is performed based on the sample dispensing system under normal conditions. The sample needle cleaning pressure waveform is obtained. The cleaning filling indicator pressure array is determined based on the cleaning pressure waveform. The filling indicator pressure array is compared with the preset filling judgment pressure range to determine whether the sample needle is in a normal filling cleaning state or an abnormal non-fill cleaning state.

[0111] The cleaning pressure waveform is used to determine the cleaning needle blockage identification pressure array. The cleaning needle blockage identification pressure array is compared with the preset cleaning needle blockage judgment pressure range to determine whether the sample needle is in normal cleaning state or needle blockage cleaning state.

[0112] It should be understood that when the processor 601 executes the sample needle aspiration anomaly detection program in the memory 602, in addition to the functions mentioned above, it can also perform other functions, as detailed in the description of the corresponding method embodiments above.

[0113] Furthermore, this embodiment of the invention does not specifically limit the type of electronic device 600 mentioned. Electronic device 600 can be a mobile phone, tablet computer, personal digital assistant (PDA), wearable device, laptop computer, or other portable electronic device. Exemplary embodiments of portable electronic devices include, but are not limited to, portable electronic devices running iOS, Android, Microsoft, or other operating systems. The aforementioned portable electronic device can also be other portable electronic devices, such as a laptop computer with a touch-sensitive surface (e.g., a touch panel). It should also be understood that in some other embodiments of the invention, electronic device 600 may not be a portable electronic device, but rather a desktop computer with a touch-sensitive surface (e.g., a touch panel).

[0114] Accordingly, this application also provides a computer-readable storage medium for storing a computer-readable program or instruction. When the program or instruction is executed by a processor, it can implement the steps or functions of the sample needle aspiration anomaly detection method provided in the above-described method embodiments.

[0115] Those skilled in the art will understand that all or part of the processes of the methods described in the above embodiments can be implemented by a computer program instructing related hardware (such as a processor, controller, etc.), and the computer program can be stored in a computer-readable storage medium. The computer-readable storage medium may be a disk, optical disk, read-only memory, or random access memory, etc.

[0116] The above provides a detailed description of the sample needle aspiration abnormality detection method, apparatus, device, and storage medium provided by the present invention. Specific examples have been used to illustrate the principles and implementation methods of the present invention. The description of the above embodiments is only for the purpose of helping to understand the method and core ideas of the present invention. At the same time, for those skilled in the art, there will be changes in the specific implementation methods and application scope based on the ideas of the present invention. Therefore, the content of this specification should not be construed as a limitation of the present invention.

Claims

1. A method for detecting abnormalities in sample needle aspiration, characterized in that, Applications in sample dispensing systems include: The sensor self-test pressure value is obtained, and the self-test pressure value is compared with the preset self-test pressure range to determine whether the sampling system is in a normal or abnormal state. The sample dispensing system is used to perform liquid aspiration operation under normal conditions. The liquid aspiration pressure waveform of the sample needle is obtained. The empty aspiration indicator pressure value is determined based on the liquid aspiration pressure waveform. The empty aspiration indicator pressure value is compared with the preset empty aspiration judgment pressure range to determine whether the sample needle is in normal condition or empty aspiration condition. The pressure value for identifying the blocked needle is determined based on the liquid aspiration pressure waveform. The pressure value for identifying the blocked needle is then compared with the preset pressure range for determining blocked needles to determine whether the sample needle is in a normal state or a blocked state. The cleaning operation is performed based on the sample dispensing system under normal conditions. The sample needle cleaning pressure waveform is obtained. The cleaning filling indicator pressure array is determined based on the cleaning pressure waveform. The filling indicator pressure array is compared with the preset filling judgment pressure range to determine whether the sample needle is in a normal filling cleaning state or an abnormal non-fill cleaning state. The cleaning pressure waveform is used to determine the cleaning needle blockage identification pressure array. The cleaning needle blockage identification pressure array is compared with the preset cleaning needle blockage judgment pressure range to determine whether the sample needle is in normal cleaning state or needle blockage cleaning state. The liquid aspiration operation includes: sample aspiration operation, reagent aspiration operation and diluent aspiration operation; The volume of liquid absorbed in the sample aspiration operation is less than a first preset volume value; the volume of liquid absorbed in the reagent aspiration operation is greater than or equal to the first preset volume value and less than a second preset volume value; and the volume of liquid absorbed in the diluent aspiration operation is greater than or equal to the second preset volume value. The process of acquiring the sample needle aspiration pressure waveform, determining the empty aspiration indicator pressure value based on the aspiration pressure waveform, and comparing the empty aspiration indicator pressure value with a preset empty aspiration judgment pressure range to determine the normal working state or empty aspiration state of the sample needle includes: Acquire the sample needle aspiration pressure waveform, which includes the sample aspiration pressure waveform, the reagent aspiration pressure waveform, and the diluent aspiration pressure waveform; The sample aspiration initiation pressure value and sample aspiration judgment pressure value are obtained based on the sample aspiration pressure waveform. The sample empty aspiration indicator pressure value is obtained by subtracting the sample aspiration initiation pressure value from the sample aspiration judgment pressure value. The sample empty aspiration indicator pressure value is compared with the preset sample empty aspiration judgment pressure range to determine the normal working state or empty aspiration state of the sample needle during sample aspiration. The reagent aspiration initiation pressure value and reagent aspiration judgment pressure value are obtained based on the reagent aspiration pressure waveform. The reagent empty aspiration indicator pressure value is obtained by subtracting the reagent aspiration initiation pressure value from the reagent aspiration judgment pressure value. The reagent empty aspiration indicator pressure value is compared with the preset reagent empty aspiration judgment pressure range to determine whether the sample needle is in normal working state or empty aspiration state during reagent aspiration. The starting pressure value and the judgment pressure value of the diluent aspiration are obtained according to the diluent aspiration pressure waveform. The diluent empty aspiration indicator pressure value is obtained by subtracting the starting pressure value of the diluent aspiration from the judgment pressure value of the diluent aspiration. The diluent empty aspiration indicator pressure value is compared with the preset diluent empty aspiration judgment pressure range to determine the normal working state or empty aspiration state of the sample needle when aspirating the diluent. The aspiration pressure waveform includes the sample aspiration pressure waveform, the reagent aspiration pressure waveform, and the diluent aspiration pressure waveform; the empty aspiration indicator pressure value includes the sample empty aspiration indicator pressure value, the reagent empty aspiration indicator pressure value, and the diluent empty aspiration indicator pressure value; and the preset empty aspiration judgment pressure range includes the preset sample empty aspiration judgment pressure range, the preset reagent empty aspiration judgment pressure range, and the preset diluent empty aspiration judgment pressure range.

2. The sample needle aspiration abnormality detection method according to claim 1, characterized in that, Determining the needle plugging indicator pressure value based on the liquid suction pressure waveform includes: The suction initiation pressure value and the suction flow rate peak pressure value are obtained based on the suction pressure waveform. The suction initiation pressure value is obtained by subtracting the suction flow rate peak pressure value from the suction flow rate peak pressure value.

3. The method for detecting abnormal sample needle aspiration according to claim 2, characterized in that, The preset needle blockage detection pressure range is the same for the sample aspiration operation, reagent aspiration operation, and diluent aspiration operation.

4. The method for detecting abnormal sample needle aspiration according to claim 1, characterized in that, The step of determining the cleaning filling indicator pressure array based on the cleaning pressure waveform includes: The cleaning start pressure value, cleaning maximum value, and cleaning minimum value are obtained based on the cleaning pressure waveform. The cleaning filling indicator pressure array is obtained by subtracting the cleaning start pressure value from the cleaning maximum value and the cleaning minimum value.

5. The method for detecting abnormal sample needle aspiration according to claim 1, characterized in that, The step of determining the cleaning needle plug identification pressure array based on the cleaning pressure waveform includes: The cleaning start pressure value, cleaning maximum pressure value, and cleaning end pressure value are obtained based on the cleaning pressure waveform. The cleaning start pressure value is then subtracted from the cleaning maximum pressure value and the cleaning end pressure value to obtain the cleaning plug needle identification pressure array.

6. A sample needle aspiration abnormality detection device, characterized in that, Applications in sample dispensing systems include: The self-test module is used to obtain the sensor self-test pressure value, and compare the self-test pressure value with the preset self-test pressure range to determine whether the sampling system is in a normal or abnormal state. The liquid aspiration empty aspiration detection module is used to perform liquid aspiration operation based on the sample addition system under normal conditions, acquire the liquid aspiration pressure waveform of the sample needle, determine the empty aspiration indicator pressure value based on the liquid aspiration pressure waveform, and compare the empty aspiration indicator pressure value with the preset empty aspiration judgment pressure range to determine whether the sample needle is in normal condition or empty aspiration state. The liquid aspiration needle blockage detection module is used to determine the needle blockage identification pressure value based on the liquid aspiration pressure waveform, and to compare the needle blockage identification pressure value with the preset needle blockage judgment pressure range to determine whether the sample needle is in a normal state or a blocked state. The cleaning and filling detection module is used to perform cleaning operations based on the sample dispensing system under normal conditions, obtain the sample needle cleaning pressure waveform, determine the cleaning and filling indicator pressure array based on the cleaning pressure waveform, and compare the filling indicator pressure array with a preset filling judgment pressure interval group to determine whether the sample needle is in a normal filling cleaning state or an abnormal non-filling cleaning state. The cleaning needle blockage detection module is used to determine the cleaning needle blockage identification pressure array based on the cleaning pressure waveform, and to compare the cleaning needle blockage identification pressure array with the preset cleaning needle blockage judgment pressure range to determine whether the sample needle is in a normal cleaning state or a blocked needle cleaning state. The liquid aspiration empty aspiration detection module includes a sample empty aspiration detection submodule, a reagent empty aspiration detection submodule, and a diluent empty aspiration detection submodule; The sample aspiration empty detection submodule is used to acquire the sample aspiration pressure waveform, acquire the sample aspiration starting pressure value and the sample aspiration judgment pressure value based on the sample aspiration pressure waveform, subtract the sample aspiration starting pressure value from the sample aspiration judgment pressure value to obtain the sample empty aspiration identification pressure value, and compare the sample empty aspiration identification pressure value with the preset sample empty aspiration judgment pressure range to determine the normal working state or empty aspiration state of the sample needle during sample aspiration. The reagent aspiration detection submodule is used to acquire the reagent aspiration pressure waveform, acquire the reagent aspiration initiation pressure value and the reagent aspiration judgment pressure value based on the reagent aspiration pressure waveform, subtract the reagent aspiration initiation pressure value from the reagent aspiration judgment pressure value to obtain the reagent aspiration aspiration identification pressure value, and compare the reagent aspiration aspiration identification pressure value with the preset reagent aspiration aspiration judgment pressure range to determine whether the sample needle is in normal working state or aspiration aspiration state during reagent aspiration. The diluent aspiration detection submodule is used to acquire the diluent aspiration pressure waveform, acquire the diluent aspiration start pressure value and the diluent aspiration judgment pressure value based on the diluent aspiration pressure waveform, subtract the diluent aspiration start pressure value from the diluent aspiration judgment pressure value to obtain the diluent aspiration aspiration indicator pressure value, and compare the diluent aspiration aspiration indicator pressure value with the preset diluent aspiration aspiration judgment pressure range to determine whether the sample needle is in normal working state or aspiration aspiration state during diluent aspiration.

7. An electronic device comprising a memory, a processor, and a computer program stored in the memory and executable on the processor, characterized in that, When the processor executes the program, it implements the sample needle aspiration abnormality detection method according to any one of claims 1 to 5.

Images