Culture device and method based on gaseous pre-priming and cyclic feeding

Abstract

The application discloses a culture device and method based on gas pre-arrangement and circulating supply, which comprises a culture bottle communicated through a first pipeline, an inlet-outlet liquid system communicated on the first pipeline, a three-way valve respectively communicated with a preloaded gas tank, a gas source communicated through a gas metering pump and the culture bottle through a second pipeline, the preloaded gas tank communicated with the culture bottle through a third pipeline, the culture bottle connected with a turbidity sensor for detecting the turbidity of liquid in the culture bottle, the preloaded gas tank connected with a pressure sensor for detecting the gas pressure in the tank, a first electromagnetic valve for controlling the communication relationship between the second pipeline and the third pipeline with each culture bottle, and a second electromagnetic valve for controlling the communication relationship between the preloaded gas tank and the third pipeline. The application realizes uninterrupted gas arrangement and supply and effectively improves the utilization rate of raw gas. The application also realizes automatic gas treatment, gas collection, culture collection and culture liquid addition and other functions under unattended conditions, saves labor cost and time cost and improves culture efficiency.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of microbial cultivation, in particular to a cultivation device and method based on gas pre-configuration and cyclic supply. BACKGROUND

[0002] The resource utilization of agricultural bulk waste such as straw is related to the sustainable development of agriculture, ecological environment and social economy. Bioconversion and synthesis technology is an effective means to realize the resource utilization of these wastes, but at present, bioconversion only focuses on the end product, and the tail gas such as CO2 has not been fully utilized, and this part of CO2 may account for about 50% of the carbon content of the whole biomass. It is of great significance to realize the high value conversion of waste and carbon emission reduction to fully utilize this half of carbon.

[0003] The existing CO2 fermentation tail gas circulating cultivation device focuses on the treatment of fermentation product CO2 tail gas, and the CO2 tail gas is directly used for circulating cultivation after simple filtration. Such a way is relatively simple and effective, but there are the following problems:

[0004] 1. Poor gas supply accuracy; when aeration, various formula gases will not enter the fermentation system according to the set flow due to liquid level pressure, resulting in unstable gas supply and inaccurate aeration amount; especially when multiple tanks are connected in parallel, due to the problem of aeration head and liquid level difference, the aeration amount of different fermentation tanks often differs greatly, and even gas leakage occurs.

[0005] 2. Narrow application range; mainly applicable to biological conversion processes with the same fermentation tail gas and cultivation gas, mainly applicable to the biological circulating cultivation of microalgae, when it is applied to the scene that needs mixed gas cultivation, because the gas circulation only contains single tail gas such as CO2, additional gas supply facilities and equipment are needed;

[0006] 3. Manual attendance is required; during the operation of the system, technical personnel need to be on site to adjust the specific parameters of each feed and gas circulation according to the cultivation process to ensure the smooth progress of the cultivation process;

[0007] 4. The tail gas is not suitable for direct use in circulating cultivation; the concentration of fermentation tail gas is generally high, and the proportion of its mixture with other components is not fixed, which cannot guarantee that the proportion of each component in the gas is suitable for cultivation during the circulation, especially when the concentration of a certain gas in the tail gas is high (such as CO2), which may have a relatively adverse effect on the cultivation process. SUMMARY

[0008] In order to solve the above technical problems, the present application provides an automatic cultivation device and method based on gas pre-configuration and cyclic supply suitable for various application scenarios.

[0009] In order to achieve the above technical purposes, the technical scheme provided by the present application comprises:

[0010] The culture device based on gas pre-arrangement and circulation supply comprises:

[0011] A plurality of culture bottles are connected through a first pipeline, and a liquid inlet and outlet system is further connected to the first pipeline;

[0012] A three-way valve is connected to a pre-filled gas tank, a gas source through a gas metering pump, and the culture bottles through a second pipeline, and a gas concentration sensor is arranged on the second pipeline;

[0013] The pre-filled gas tank is further connected to the culture bottles through a third pipeline, and a circulation pump is arranged on the third pipeline;

[0014] The culture bottles are connected to a turbidity sensor for detecting the turbidity of the liquid in the bottles and a first liquid level sensor for detecting the liquid level in the bottles;

[0015] The pre-filled gas tank is connected to a pressure sensor for detecting the gas pressure in the tank and a second liquid level sensor for detecting the liquid level in the tank;

[0016] The pre-filled gas tank is further provided with a first liquid outlet end and an exhaust end;

[0017] A first electromagnetic valve is used to control the communication relationship between the second pipeline and the third pipeline and the culture bottles;

[0018] A second electromagnetic valve is used to control the communication relationship between the pre-filled gas tank and the third pipeline;

[0019] One end of the second pipeline connected to the culture bottles is located above the culture liquid level; one end of the first pipeline and the third pipeline connected to the culture bottles is located below the culture liquid level.

[0020] In some preferred embodiments, the liquid inlet and outlet system comprises a liquid inlet end and a second liquid outlet end which are parallel and connected to the first pipeline; the liquid inlet end and the second liquid outlet end are respectively provided with electromagnetic valves and liquid metering pumps.

[0021] In some preferred embodiments, one end of the third pipeline inserted into the culture bottles is provided with an aeration head.

[0022] In some preferred embodiments, an adjustable damper is arranged on the third pipeline in front of the culture bottles and behind the first electromagnetic valve.

[0023] The present application also provides a culture method based on gas pre-arrangement and circulation supply, comprising:

[0024] S1, a predetermined amount of culture liquid is added into a plurality of culture bottles through a liquid inlet and outlet system;

[0025] S2, the three-way valve is conducted to the pre-filled gas tank and the gas source, and a certain amount of raw gas is added to the pre-filled gas tank through the gas source;

[0026] S3, the three-way valve is conducted to the pre-filled gas tank and the second pipeline, the first electromagnetic valve and the second electromagnetic valve are opened, the circulating pump is started, and the gas flows in the order of the pre-filled gas tank, the third pipeline, the culture bottle, the second pipeline and the three-way valve;

[0027] S4, when the detection value of the gas concentration sensor reaches the predetermined value, the circulating pump is closed, the exhaust end is opened and the exhaust gas is collected, when the tank pressure returns to atmospheric pressure, the exhaust end is closed, and steps S2-S4 are repeatedly executed.

[0028] In some preferred embodiments, the method further comprises the steps of:

[0029] When the detection value of the turbidity sensor reaches the predetermined value, the first electromagnetic valve is controlled to keep the third pipeline and the culture bottle open, and the second pipeline and the culture bottle are closed; a certain amount of old culture solution is discharged through the liquid inlet and outlet system, and the same amount of new culture solution is supplemented; after the supplement is completed, the first electromagnetic valve is controlled to keep the third pipeline and the culture bottle open, and the second pipeline and the culture bottle are opened.

[0030] In some preferred embodiments, the method further comprises the steps of:

[0031] When the second liquid level sensor detects that the liquid level in the pre-filled gas tank reaches the preset, the liquid in the tank is discharged through the first liquid outlet; when the pressure sensor detects that the gas pressure in the pre-filled gas tank reaches the preset, the exhaust end is opened to reduce the pressure in the tank.

[0032] Advantages

[0033] 1. The present application can realize uninterrupted gas preparation and supply, and effectively improve the utilization rate of raw gas by batch processing of gas through the pre-filled gas tank and the circulating pipeline; 2. Avoid the problem that the formula gas is not supplied according to the set flow rate due to liquid level pressure, which can improve the uniform distribution of gas in the liquid, especially under the condition that stirring is not allowed; 3. The damper effectively balances the inlet pressure of each tank, which realizes consistent aeration of each tank through simple means without significantly increasing economic cost, and guarantees continuous and stable operation; 4. By presetting the parameters of each sensor and cooperating with the circulating pipeline, the functions of automatic gas treatment, gas collection, culture collection and culture solution addition can be realized under unattended condition, which saves labor cost and time cost, and improves culture efficiency; 5. Modular components are adopted, which can be freely combined according to actual needs to realize customized functions and processing capacity. BRIEF DESCRIPTION OF DRAWINGS

[0034] Figure 1 It is a structure schematic diagram of a culture device based on gas pre-preparation and circulating supply in a preferred embodiment of the present application.

[0035] Figure 2 This is a schematic diagram of a cultivation method based on gas pre-mixing and circulating supply in another preferred embodiment of the present invention.

[0036] In the diagram: 1. Culture flask; 2. Liquid inlet / outlet system; 3. Three-way valve; 4. Pre-filled gas tank; 5. Gas source; 6. Gas metering pump; 7. Second pipeline; 8. Gas concentration sensor; 9. Third pipeline; 10. Circulation pump; 11. Turbidity sensor; 12. First pipeline; 13. First liquid level sensor; 14. Pressure sensor; 15. Second liquid level sensor; 16. First liquid outlet; 17. Exhaust end; 18. First solenoid valve; 19. Second solenoid valve; 20. Safety valve; 21. Damper; 22. Liquid metering pump; 101. Aeration head; 201. Liquid inlet; 202. Second liquid outlet; Detailed Implementation

[0037] To make the objectives, technical solutions, and advantages of this invention clearer, the invention will be further described below with reference to the accompanying drawings. In the description of this invention, it should be understood that the terms "upper," "lower," "front," "rear," "left," "right," "top," "bottom," "inner," and "outer," etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings, and are only for the convenience of describing the invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of the invention.

[0038] like Figure 1 As shown, this embodiment provides a culture device based on gas pre-mixing and circulating supply, including:

[0039] Several culture flasks 1 are connected through a first pipeline 12, which is also connected to an inlet / outlet system 2. The number of culture flasks 1 can be reasonably set by those skilled in the art according to the actual culture volume requirements. The flasks can be made of pressure-resistant transparent material or metal containers, and can be installed on a base with functions such as temperature control, illumination, and stirring. Other supporting devices and equipment for monitoring and controlling indicators such as pH and ion concentration can be added. The inlet / outlet system 2 is for replenishing and / or collecting the culture medium in the culture flasks 1, and its specific implementation can be set by those skilled in the art based on common replenishment and dispensing methods. In some preferred embodiments, a method of implementing inlet / outlet flow through separate channels is provided, specifically including:

[0040] The liquid inlet / outlet system 2 includes a liquid inlet end 201 and a liquid outlet end 202 that are parallel to each other and connected to the first pipeline 12; the liquid inlet end 201 and the liquid outlet end 202 are respectively equipped with a solenoid valve and a liquid metering pump 22.

[0041] A three-way valve 3 is connected with the pre-charged gas tank 4, the gas source 5 through the gas metering pump 6, and the culture bottle 1 through the second pipeline 7, and the gas concentration sensor 8 is arranged on the second pipeline 7;

[0042] The pre-charged gas tank 4 is also connected with the culture bottle 1 through the third pipeline 9, and the circulating pump 10 is arranged on the third pipeline 9;

[0043] The culture bottle 1 is connected with the turbidity sensor 11 for detecting the turbidity of the liquid in the bottle and the first liquid level sensor 13 for detecting the liquid level in the bottle;

[0044] The pre-charged gas tank 4 is connected with the pressure sensor 14 for detecting the gas pressure in the tank and the second liquid level sensor 15 for detecting the liquid level in the tank;

[0045] The pre-charged gas tank 4 is also provided with the first liquid outlet end 16 and the gas outlet end 17; wherein the first liquid outlet end 16 can control the liquid outlet process and the liquid outlet amount through the electromagnetic valve; the gas outlet end 17 includes the electromagnetic valve and the gas metering pump 6 connected with the electromagnetic valve, for controlling the gas outlet process and the gas outlet volume of the pre-charged gas tank 4.

[0046] The first electromagnetic valve 18 is used for controlling the communication relationship between the second pipeline 7 and the third pipeline 9 and each culture bottle 1;

[0047] The second electromagnetic valve 19 is used for controlling the communication relationship between the pre-charged gas tank 4 and the third pipeline 9;

[0048] Wherein, one end of the second pipeline 7 connected with the culture bottle 1 is located above the culture liquid surface; one end of the first pipeline 12 and the third pipeline 9 connected with the culture bottle 1 is located below the culture liquid surface. In some preferable embodiments, in order to increase the aeration amount of the gas into the culture liquid, the aeration head 101 is arranged at the end of the third pipeline 9 extending into the culture bottle 1.

[0049] In another preferable embodiment, in order to ensure the consistent gas inlet amount of each culture bottle 1, the adjustable damper 21 is arranged on the third pipeline 9 before the culture bottle 1 and after the first electromagnetic valve 18.

[0050] It should be understood that, in order to realize the data acquisition and processing of the above-mentioned various sensors and set the opening and closing of each electromagnetic valve and pump device according to the related parameters, the controller capable of realizing the above-mentioned functions should also be obviously provided in the present application, which is electrically connected with the various sensors, electromagnetic valves and pump devices to realize the control of the whole culture process.

[0051] The working process and principle of the present embodiment are described as follows:

[0052] 1. Initial State Setting: Calculate the required volume of culture medium to be added based on the volume of each culture bottle 1, and set the liquid level detection value of the first liquid level sensor 13 to the corresponding parameter; calculate the required volume of raw material gas to be added to the pre-filled gas tank 4 based on the available space for gas in the entire system, and set the gas flow rate parameter of the gas metering pump 6 and the pressure sensor 14 parameter in the pre-filled gas tank 4 accordingly; set the liquid turbidity and sampling volume according to the actual needs on site, and set the lower limit of gas concentration to trigger gas replenishment according to the evolution of the culture process; set the liquid level detection value of the second liquid level sensor 15 based on the volume of the pre-filled gas tank 4 to check the volume of liquid condensed in the pre-filled gas tank 4. When the volume of condensed liquid reaches the liquid level detection value of the second liquid level sensor 15, the liquid in the pre-filled gas tank 4 needs to be discharged.

[0053] 2. Adding culture medium: The three-way valve 3 connects the second pipeline 7 and the pre-filled gas tank 4, the second solenoid valve 19 is turned on, the first solenoid valve 18 connects the second pipeline 7 and the culture bottle 1, the third pipeline 9 and the culture bottle 1 are closed, the first liquid outlet 16 is opened, and the preset volume of culture medium is added to each culture bottle 1 through the liquid inlet / outlet system 2; after the liquid addition is completed, the first liquid outlet 16 is closed.

[0054] 3. Pressure balancing of gas pipeline: Open the exhaust end 17 on the pre-installed gas tank 4 to balance the pressure inside and outside the tank, and collect the discharged gas and send it to the next stage of processing or storage device; after balancing, close the exhaust end 17 on the pre-installed gas tank 4.

[0055] 4. Addition of raw material gas: Close the first solenoid valve 18, the second solenoid valve 19 and the circulation pump 10, the three-way valve 3 connects the gas source 5 and the pre-filled gas tank 4, and the gas metering pump 6 adds the preset volume of raw material gas to the pre-filled gas tank 4.

[0056] 5. Gas circulation culture: After gas addition is completed, the three-way valve 3 connects the pre-filled gas tank 4 and the second pipeline 7, the second solenoid valve 19 is opened, and the first solenoid valve 18 is opened to connect the second pipeline 7 and the third pipeline 9 to each culture bottle 1 respectively. The circulation pump 10 is started. At this time, the gas will circulate between the culture bottle 1 and the pre-filled gas tank 4.

[0057] 6. Raw material gas replenishment: When the gas concentration sensor 8 detects that the gas concentration is lower than the preset value, repeat step 3-5.

[0058] 7. Extraction of culture medium: When the turbidity sensor 11 detects that the turbidity of the culture medium reaches the preset value, the first solenoid valve 18 closes the connection between the second pipeline 7 and each culture bottle 1, and uses the inlet and outlet liquid system 2 to extract the preset volume of old culture medium and add the corresponding volume of new culture medium. After the liquid is added again, the first solenoid valve 18 opens the connection between the second pipeline 7 and each culture bottle 1.

[0059] 8. Condensate water discharge: according to the volume of the pre-charging tank 4, set the appropriate condensate water level at the first liquid level sensor 13, when the condensate water in the pre-charging tank 4 reaches the preset height, open the first liquid outlet 16, discharge the condensate water in the tank, to avoid the condensate water storage in the tank.

[0060] 9. Pre-charging tank 4 pressure protection: when the pressure sensor 14 in the pre-charging tank 4 detects that the pressure in the tank is greater than the preset value, open the exhaust end 17 on the pre-charging tank 4, balance the pressure inside and outside the tank, and collect the discharged gas to the next level processing device or storage device; after balancing, close the exhaust end 17 on the pre-charging tank 4. In some preferred embodiments, in order to prevent overpressure caused by failure of the pressure sensor 14, a safety valve 20 can also be provided on the pre-charging tank 4.

[0061] 10. Formula gas pre-mixing configuration: when the culture process requires multiple component raw gas supply, close the first electromagnetic valve 18, the second electromagnetic valve 19 and the circulating pump 10, the three-way valve 3 is connected to the gas source 5 and the pre-charging tank 4, and the gas metering pump 6 adds different kinds of raw gas with preset volume into the pre-charging tank 4 respectively, to realize accurate configuration of formula gas.

[0062] Embodiment 2

[0063] This embodiment is based on the above-mentioned embodiment 1, as shown in the figure, this embodiment gives a culture method based on gas pre-configuration and circulating supply, including: Figure 2

[0064] S1, add a certain amount of culture solution to a plurality of culture bottles 1 through the inlet and outlet system 2;

[0065] S2, connect the pre-charging tank 4 and the gas source 5 through the three-way valve 3, and add a certain amount of raw gas to the pre-charging tank 4 through the gas source 5;

[0066] S3, connect the pre-charging tank 4 and the second pipeline 7 through the three-way valve 3, open the first electromagnetic valve 18 and the second electromagnetic valve 19, and start the circulating pump 10, so that the gas circulates in the order of pre-charging tank 4, third pipeline 9, culture bottle 1, second pipeline 7 and three-way valve 3;

[0067] S4, when the detection value of the gas concentration sensor 8 reaches the predetermined value, close the circulating pump 10, open the exhaust end 17 and collect the exhaust gas, and when the pressure in the tank returns to atmospheric pressure, close the exhaust end 17, and repeat steps S2-S4.

[0068] In other preferred embodiments, a specific method for automatically controlling the discharge and introduction of old culture solution and new culture solution is also given, including the steps of:

[0069] ​When the detection value of the turbidity sensor 11 reaches a predetermined value, the first electromagnetic valve 18 is controlled to keep the third pipeline 9 open to the culture bottle 1 and the second pipeline 7 closed to the culture bottle 1; a certain amount of old culture solution is discharged through the liquid inlet and outlet system 2, and the same amount of new culture solution is supplemented; after the supplement is completed, the first electromagnetic valve 18 is controlled to keep the third pipeline 9 open to the culture bottle 1 and the second pipeline 7 open to the culture bottle 1.

[0070] In other preferred embodiments, a method for controlling the volume of liquid and gas in the pre-filled gas tank 4 is given, which specifically comprises the following steps:

[0071] When the second liquid level sensor 15 detects that the liquid level in the pre-filled gas tank 4 reaches a preset value, the liquid in the tank is discharged through the first liquid outlet 16; when the pressure sensor 14 detects that the gas pressure in the pre-filled gas tank 4 reaches a preset value, the gas outlet 17 is opened to reduce the pressure in the tank.

[0072] Embodiment 3

[0073] This embodiment is based on the above-mentioned embodiments 1 and 2. The culture method and device based on gas pre-distribution and cyclic supply provided by the present application are suitable for distributing the concentration and proportion of one or more gases, and are applied to the culture of bacteria, yeast, edible fungi, algae and other biological cultures, as well as chemical reactions that require mixed gas preparation. Two specific application scenarios are given below:

[0074] Application scenario one

[0075] The culture method and device based on gas pre-distribution and cyclic supply provided by the present application can be used to prepare single gases with different concentrations for microalgae culture. High-purity carbon dioxide (CO2) gas is accurately prepared into different concentrations of 0.04%, 4% and 40%, and one concentration is opened or closed within a set time period for the culture of microalgae A; or three concentrations are opened or closed within a set time period according to the set requirements, and the microalgae culture solution is sequentially aerated, finally realizing the cyclic supply, efficient utilization of CO2 gas and high-efficiency growth of microalgae.

[0076] Application scenario two

[0077] The culture method and device based on gas pre-distribution and cyclic supply provided by the present application can be used to mix multiple gases in proportion for microbial culture. High-purity carbon dioxide (CO2) gas and hydrogen (H2) are mixed in the required proportion for the culture of bacteria B. Uninterrupted preparation and cyclic supply of gas are realized.

[0078] The above shows and describes the basic principles, main features and advantages of the present application. Those skilled in the art should understand that the present application is not limited to the above-mentioned embodiments, and the above-mentioned embodiments and descriptions in the specification are only to illustrate the principles of the present application. Without departing from the spirit and scope of the present application, various changes and improvements can be made to the present application, and these changes and improvements all fall within the scope of the claimed present application. The scope of protection of the present application is defined by the appended claims and their equivalents.

Claims

1. A cultivation method based on a gas pre-preparation and circulating supply cultivation device, characterized in that, Includes the following steps: (1) Initial state setting: Calculate the required volume of culture medium to be added based on the volume of each culture bottle (1), and set the liquid level detection value of the first liquid level sensor (13) as the corresponding parameter; calculate the volume of raw material gas to be added to the pre-filled gas tank (4) based on the available space for gas in the entire system, and set the gas flow parameters of the gas metering pump (6) and the parameters of the pressure sensor (14) in the pre-filled gas tank (4) accordingly; set the liquid turbidity and the volume of material to be taken according to the actual needs of the site, and set the lower limit of the gas concentration to trigger gas replenishment according to the evolution of the culture process; set the liquid level detection value of the second liquid level sensor (15) based on the volume of the pre-filled gas tank (4) to check the volume of liquid condensed in the pre-filled gas tank (4). When the volume of condensed liquid reaches the liquid level detection value of the second liquid level sensor (15), the liquid in the pre-filled gas tank (4) needs to be discharged. (2) Addition of culture medium: The three-way valve (3) connects the second pipeline (7) and the pre-filled gas tank (4), the second solenoid valve (19) connects the third pipeline (9) and the pre-filled gas tank (4), and the first solenoid valve (18) connects the second pipeline (7) and the culture bottle (1); the third pipeline (9) and the culture bottle (1) are closed, the first liquid outlet (16) is opened, and the preset culture medium volume is added to each culture bottle (1) through the liquid inlet and outlet system (2); After the liquid addition is completed, close the first liquid outlet (16); (3) Pressure balancing of gas pipeline: Open the exhaust end (17) on the pre-installed gas tank (4) to balance the pressure inside and outside the tank, and collect the discharged gas and send it to the next stage of processing device or storage device; after balancing, close the exhaust end (17) on the pre-installed gas tank (4). (4) Addition of raw material gas: Close the first solenoid valve (18), the second solenoid valve (19) and the circulation pump (10), the three-way valve (3) connects the gas source (5) and the pre-filled gas tank (4), and the gas metering pump (6) adds the preset volume of raw material gas to the pre-filled gas tank (4). (5) Gas circulation culture: After the gas is added, the three-way valve (3) connects the pre-filled gas tank (4) and the second pipeline (7), the second solenoid valve (19) is opened, and the first solenoid valve (18) is opened to connect the second pipeline (7) and the third pipeline (9) to each culture bottle (1) respectively. The circulation pump (10) is started. At this time, the gas will circulate between the culture bottle (1) and the pre-filled gas tank (4). (6) Replenishment of raw material gas: When the gas concentration sensor (8) detects that the gas concentration is lower than the preset value, repeat steps (3)-(5); (7) Extraction of culture medium: When the turbidity sensor (11) detects that the turbidity of the culture medium reaches the preset value, the first solenoid valve (18) closes the connection between the second pipeline (7) and each culture bottle (1), and the inlet and outlet liquid system (2) extracts the preset volume of old culture medium and replenishes the corresponding volume of new culture medium. After the liquid is replenished, the first solenoid valve (18) opens the connection between the second pipeline (7) and each culture bottle (1); (8) Condensate discharge: Set an appropriate condensate level on the second liquid level sensor (15) according to the volume of the pre-installed gas tank (4). When the condensate in the pre-installed gas tank (4) reaches the preset height, open the first liquid outlet (16) to discharge the condensate in the tank to avoid excessive condensate accumulation in the tank. (9) Pressure protection of pre-installed gas tank (4): When the pressure sensor (14) in the pre-installed gas tank (4) detects that the pressure inside the tank is greater than the preset value, the exhaust end (17) on the pre-installed gas tank (4) is opened to balance the pressure inside and outside the tank, and the discharged gas is collected and sent to the next stage processing device or storage device; after the balance is completed, the exhaust end (17) on the pre-installed gas tank (4) is closed. A safety valve (20) may be installed on the pre-installed gas tank (4). (10) Premixing of formulation gas: When the cultivation process requires the supply of raw materials gas of multiple components, the first solenoid valve (18), the second solenoid valve (19) and the circulation pump (10) are closed, the three-way valve (3) connects the gas source (5) and the pre-filled gas tank (4), and the gas metering pump (6) adds different types of raw materials gas of each preset volume to the pre-filled gas tank (4) to achieve precise configuration of formulation gas; The culture device based on gas pre-mixing and circulating supply includes: Several culture flasks (1) are connected through a first pipeline (12), and an inlet / outlet liquid system (2) is also connected to the first pipeline (12); A three-way valve (3) is connected to a pre-filled gas tank (4), a gas source (5) via a gas metering pump (6), and a culture bottle (1) via a second pipeline (7). A gas concentration sensor (8) is installed on the second pipeline (7). The pre-filled gas tank (4) is also connected to the culture bottle (1) through a third pipeline (9), on which a circulation pump (10) is installed; The culture flask (1) is connected to a turbidity sensor (11) for detecting the turbidity of the liquid inside the flask, and a first liquid level sensor (13) for detecting the height of the liquid inside the flask; The pre-filled gas tank (4) is connected to a pressure sensor (14) for detecting the gas pressure inside the tank and a second liquid level sensor (15) for detecting the liquid level inside the tank; The pre-filled gas tank (4) is also provided with a first liquid outlet (16) and an exhaust outlet (17); The first solenoid valve (18) is used to control the connection between the second pipeline (7) and the third pipeline (9) and each culture flask (1); The second solenoid valve (19) is used to control the connection between the pre-installed gas tank (4) and the third pipeline (9); Wherein, one end of the second pipe (7) connected to the culture bottle (1) is above the culture medium surface; one end of the first pipe (12) and the third pipe (9) connected to the culture bottle (1) is below the culture medium surface; The liquid inlet and outlet system (2) includes a liquid inlet end (201) and a liquid outlet end (202) that are parallel to each other and connected to the first pipeline (12); the liquid inlet end (201) and the liquid outlet end (202) are respectively equipped with a solenoid valve and a liquid metering pump (22); The third pipeline (9) is equipped with an aeration head (101) at one end that extends into the culture bottle (1); An adjustable damper (21) is installed on the third pipeline (9) before the culture bottle (1) and after the first solenoid valve (18).

2. A culture device based on gas pre-mixing and circulating supply, comprising: Several culture flasks (1) are connected through a first pipeline (12), and an inlet / outlet liquid system (2) is also connected to the first pipeline (12); A three-way valve (3) is connected to a pre-filled gas tank (4), a gas source (5) via a gas metering pump (6), and a culture bottle (1) via a second pipeline (7). A gas concentration sensor (8) is installed on the second pipeline (7). The pre-filled gas tank (4) is also connected to the culture bottle (1) through a third pipeline (9), on which a circulation pump (10) is installed; The culture flask (1) is connected to a turbidity sensor (11) for detecting the turbidity of the liquid inside the flask, and a first liquid level sensor (13) for detecting the height of the liquid inside the flask; The pre-filled gas tank (4) is connected to a pressure sensor (14) for detecting the gas pressure inside the tank and a second liquid level sensor (15) for detecting the liquid level inside the tank; The pre-filled gas tank (4) is also provided with a first liquid outlet (16) and an exhaust outlet (17); The first solenoid valve (18) is used to control the connection between the second pipeline (7) and the third pipeline (9) and each culture flask (1); The second solenoid valve (19) is used to control the connection between the pre-installed gas tank (4) and the third pipeline (9); Wherein, one end of the second pipe (7) connected to the culture bottle (1) is above the culture medium surface; one end of the first pipe (12) and the third pipe (9) connected to the culture bottle (1) is below the culture medium surface; The liquid inlet and outlet system (2) includes a liquid inlet end (201) and a liquid outlet end (202) that are parallel to each other and connected to the first pipeline (12); the liquid inlet end (201) and the liquid outlet end (202) are respectively equipped with a solenoid valve and a liquid metering pump (22); The third pipeline (9) is equipped with an aeration head (101) at one end that extends into the culture bottle (1); An adjustable damper (21) is installed on the third pipeline (9) before the culture bottle (1) and after the first solenoid valve (18).

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