Intelligent bioreactor integrated with multi-parameter biochemical analysis function

Abstract

The utility model discloses an intelligent bioreactor integrated with a multi-parameter biochemical analysis function. The intelligent bioreactor comprises an optical detection module, an electrochemical sensor module and an overflow pool biochemical detection module, the optical detection module adopts a transmission-type optical measurement principle and is used for measuring the optical density of the culture solution; the electrochemical sensor module adopts an electrochemical principle and is used for detecting the concentration of at least 10 metabolites in the culture solution; the overflow pool biochemical detection module adopts an ion selective electrode principle and is used for simultaneously detecting the concentrations and pH values of NH4 < + >, K < + >, Na < + > and Ca < 2 + > ions in the culture solution; the three modules are sequentially connected through fluid channels to form a complete detection flow path.

Description

Technical Field

[0001] This utility model relates to the field of bioreactor technology, and in particular to an intelligent bioreactor that integrates multi-parameter biochemical analysis functions. Background Technology

[0002] In modern biotechnology and pharmaceutical industries, bioreactors are key equipment, and their performance directly affects the efficiency and quality of microbial cultivation and product production. Traditional bioreactors typically only have limited monitoring functions, such as detecting basic parameters like temperature, pH, and dissolved oxygen. Monitoring critical parameters such as microbial concentration, metabolite composition, and ionic environment in the culture medium often relies on manual sampling and offline analysis, which is not only inefficient but also unable to achieve real-time monitoring and timely control, severely restricting the development of the biotechnology industry and the improvement of product quality.

[0003] In recent years, with the development of biosensing technology, some improved bioreactors have begun to adopt single-type online detection technologies, such as using optical detection to achieve real-time monitoring of cell concentration, or using electrochemical sensors to detect changes in the concentration of specific metabolites. However, these improvements still have significant shortcomings: on the one hand, single-type detection technologies can only monitor specific categories of parameters and cannot provide comprehensive information; on the other hand, different detection technologies are usually used as independent modules, making it difficult to achieve simultaneous detection and correlation analysis of multiple parameters, thus making it difficult for researchers to fully understand the interactions and synergistic changes among various parameters during microbial culture.

[0004] Furthermore, existing technologies lack solutions that effectively integrate optical detection, electrochemical sensing, and ion detection technologies. Poor interface compatibility between detection modules, inconsistent data formats, and difficulties in establishing a unified data acquisition and processing platform are also issues. Simultaneously, traditional detection systems are mostly fixed installations, lacking remote monitoring and intelligent control capabilities, requiring researchers to be stationed in the laboratory for extended periods, resulting in low work efficiency. These technological bottlenecks severely restrict the precise control and optimization of bioreaction processes, impacting the R&D efficiency and production quality of biotechnology products. Therefore, there is an urgent need to develop an intelligent bioreactor capable of multi-parameter biochemical analysis, providing a more comprehensive, accurate, and real-time monitoring method for the biotechnology field. Utility Model Content

[0005] The purpose of this invention is to address the shortcomings of existing technologies by proposing an intelligent bioreactor that integrates multi-parameter biochemical analysis functions.

[0006] To achieve the above objectives, the present invention adopts the following technical solution:

[0007] An intelligent bioreactor integrating multi-parameter biochemical analysis functions includes:

[0008] The optical detection module 100 is used to measure the optical density of the culture medium. The optical detection module 100 adopts the principle of transmission optical measurement and determines the optical density of the sample by measuring the intensity change of light before and after passing through the sample.

[0009] Electrochemical sensor module 200 is used to detect the concentration of at least 10 metabolites in culture medium. Electrochemical sensor module 200 adopts the principle of electrochemistry and detects the concentration of specific metabolites in the sample by measuring the change of potential difference between electrodes.

[0010] The overflow tank biochemical detection module 300 is used to simultaneously detect the concentration of at least four ions, namely NH4+, K+, Na+, and Ca2+, and the pH value in the culture medium. The overflow tank biochemical detection module 300 adopts the principle of ion selective electrode.

[0011] The optical detection module 100, the electrochemical sensor module 200, and the overflow cell biochemical detection module 300 are connected in sequence through a fluid channel to form a complete detection flow path.

[0012] Furthermore, the optical detection module 100 includes:

[0013] Lenses 1 are mounted on both sides of the device;

[0014] Red light 2 serves as the light source;

[0015] The red filter 3 is located in the optical path;

[0016] First sample receiving pool 4 for holding the sample to be tested;

[0017] Receiver 5 is used to receive optical signals.

[0018] Furthermore, the electrochemical sensor module 200 includes:

[0019] Overflow cap 6 for liquid overflow protection;

[0020] A second sample receiving pool 11 for holding the sample to be tested;

[0021] Platinum wire fixing shell 7 for fixing electrodes;

[0022] Platinum wire 8 serving as the working electrode;

[0023] Silver sheet 9 serving as reference electrode;

[0024] The first sealing ring 10 is used for system sealing.

[0025] Furthermore, the overflow pool biochemical detection module 300 includes:

[0026] Silver chloride rod holder 12 for fixing electrodes;

[0027] The first outer shell 13, the second outer shell 14, the third outer shell 15, the fourth outer shell 16, and the fifth outer shell 17 constitute the external structure of the ion detection unit;

[0028] Silver chloride rod 18 serves as a reference electrode;

[0029] 19, an internal electrolyte chamber for holding internal electrolyte;

[0030] Second sealing ring 20 for system sealing;

[0031] Inlet 21 and outlet 22 are used for sample entry and exit.

[0032] Furthermore, the overflow pool biochemical detection module 300 includes multiple ion-selective electrodes for detecting the concentrations of NH4+, K+, Na+, Ca2+ ions and pH value.

[0033] Furthermore, the bioreactor also includes an intelligent control unit, which is electrically connected to the optical detection module 100, the electrochemical sensor module 200, and the overflow pool biochemical detection module 300. The intelligent control unit is used to collect and process detection data and automatically adjust the operating parameters of the bioreactor based on the detection results.

[0034] Furthermore, the intelligent control unit supports wireless data transmission and remote monitoring.

[0035] Furthermore, the optical detection module uses red light with a wavelength of 630nm as the light source.

[0036] Furthermore, the electrochemical sensor module can detect one or more metabolites, including glucose, lactic acid, ethanol, glycerol, and glutamate.

[0037] The beneficial effects of this utility model are as follows:

[0038] 1. This utility model relates to an intelligent bioreactor integrating multi-parameter biochemical analysis. Through innovative integration of optical, electrochemical, and ion detection technologies, it achieves real-time synchronous detection of microbial concentration (OD value), concentrations of more than 10 metabolites, concentrations of four key ions (NH4+, K+, Na+, Ca2+), and pH value in the culture medium. This solves the technical problems of traditional bioreactors, such as single monitoring parameters, discontinuous detection, and the inability to perform multi-parameter correlation analysis. This design significantly improves the comprehensiveness and real-time nature of bioreactor monitoring, enabling researchers to fully grasp the changes in various key indicators during microbial culture, providing a reliable basis for precise control. It also supports wireless data transmission and remote monitoring, greatly reducing the workload of manual sampling and offline analysis, improving experimental efficiency and safety, and has significant practical value for improving the technological level of bio-fermentation, pharmaceutical industry, and scientific research.

[0039] The above description is merely an overview of the technical solution of this utility model. In order to better understand the technical means of this utility model and to implement it in accordance with the contents of the specification, and to make the above and other objects, features and advantages of this utility model more apparent and understandable, preferred embodiments are described below in detail with reference to the accompanying drawings. Attached Figure Description

[0040] Figure 1 This is a schematic diagram of the optical detection module structure of an intelligent bioreactor integrating multi-parameter biochemical analysis function proposed in this utility model;

[0041] Figure 2 This is a schematic diagram of the electrochemical sensor module structure of an intelligent bioreactor integrating multi-parameter biochemical analysis function proposed in this utility model.

[0042] Figure 3 This is a schematic diagram of the overflow pool biochemical detection module of an intelligent bioreactor that integrates multi-parameter biochemical analysis functions, as proposed in this utility model.

[0043] In the diagram: 100, Optical detection module; 200, Electrochemical sensor module; 300, Overflow cell biochemical detection module; 1, Lens; 2, Red light; 3, Red filter; 4, First sample receiving cell; 5, Receiver; 6, Overflow cap; 7, Platinum wire fixing shell; 8, Platinum wire; 9, Silver sheet; 10, Sealing ring; 11, Second sample receiving cell; 12, Silver chloride rod fixing seat; 13, First outer shell; 14, Second outer shell; 15, Third outer shell; 16, Fourth outer shell; 17, Fifth outer shell; 18, Silver chloride rod; 19, Ion filling chamber; 21, Liquid inlet; 22, Liquid outlet. Detailed Implementation

[0044] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments of the present utility model. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments.

[0045] This invention provides an intelligent bioreactor integrating multi-parameter biochemical analysis functions. The reactor mainly consists of three parts: an optical detection module, an electrochemical sensor module, and an overflow pool biochemical detection module. It realizes real-time synchronous detection of optical density, multiple metabolites, multiple ions, and pH value, which greatly improves the comprehensiveness and real-time performance of biological reaction process monitoring.

[0046] I. Structure of Optical Detection Module

[0047] like Figure 1 As shown, the optical detection module mainly includes:

[0048] Lenses 1 are positioned on both sides of the device to focus the light beam; red lamp 2 serves as the light source, generating red light of a specific wavelength; red filter 3 is located in the optical path to filter light of a specific wavelength, ensuring measurement accuracy; the first sample receiving cell 4 is used to hold the sample to be tested; and receiver 5 is located at the other end of the optical path to receive the light signal after it has passed through the sample. This structure employs the principle of transmission optical measurement, determining the optical density of the sample by measuring the change in light intensity before and after it passes through the sample, thereby reflecting the concentration of microorganisms in the culture medium.

[0049] II. Electrochemical Sensor Module Structure

[0050] like Figure 2 As shown, the electrochemical sensor module mainly includes:

[0051] Overflow cap 6 is used for liquid overflow protection; second sample receiving pool 11 is used to hold the sample to be tested; platinum wire fixing shell 7 is used to fix and protect platinum wire electrode; platinum wire 8 serves as working electrode; silver sheet 9 serves as reference electrode; first sealing ring 10 ensures system sealing and prevents liquid leakage. This module uses electrochemical principles to detect the concentration of specific metabolites in the sample by measuring the potential difference change between electrodes.

[0052] III. Structure of Overflow Pool Biochemical Detection Module

[0053] like Figure 3 As shown, the overflow pool biochemical detection module mainly includes:

[0054] The silver chloride rod holder 12 is used to fix the silver chloride reference electrode; the first outer shell 13, the second outer shell 14, the third outer shell 15, the fourth outer shell 16 and the fifth outer shell 17 constitute the external structure of the ion detection unit; the silver chloride rod 18 serves as the reference electrode for each ion sensor; the ion internal filling chamber 19 is used to hold the internal electrolyte; the second sealing ring 20 ensures the system is sealed; the inlet 21 and the outlet 22 are used for sample entry and exit, respectively.

[0055] like Figure 3 As shown, this module can simultaneously detect NH4+, K+, Na+, Ca2+ and pH value, and is equipped with a reference electrode (Ref) to achieve real-time monitoring of the concentration of multiple key ions during the culture process.

[0056] Example

[0057] Example 1: Principle and Application of Optical Module Detection

[0058] In this embodiment, the optical detection module uses 630nm wavelength red light as the light source. After being filtered by a red filter 3, the light beam passes through the culture medium sample in the first sample receiving tank 4 and is finally received by the receiver 5. By comparing the incident light intensity and the transmitted light intensity, the optical density value of the culture medium is calculated. This value is positively correlated with the microbial concentration, enabling real-time monitoring of the microbial growth status in the bioreactor.

[0059] In practical applications, this module can automatically collect culture medium samples, measure the OD value every 5 minutes, and transmit the data to the control system to achieve real-time monitoring of the fermentation process. When the OD value reaches a preset threshold, the system can automatically trigger the next operation, such as feeding or harvesting.

[0060] Example 2: Implementation of the electrochemical sensor module

[0061] In this embodiment, the electrochemical sensor module comprises a platinum wire 8 and a silver sheet 9 forming an electrochemical detection unit. When the culture medium passes through the second sample receiving cell 11, the platinum wire serves as the working electrode, and the silver sheet as the reference electrode. At an appropriate potential, metabolites (including glucose, lactic acid, etc.) undergo oxidation or reduction reactions on the electrode surface, generating a current signal. This signal is proportional to the metabolite concentration, and the concentration value of the metabolite can be obtained through signal processing.

[0062] This module can detect more than 10 metabolites, including glucose, lactic acid, ethanol, glycerol, glutamate, etc., with a sensitivity of up to 0.1mM, providing comprehensive metabolic state monitoring for biological reaction processes.

[0063] Example 3: Working process of the ion detection module

[0064] like Figure 3 As shown, the ion detection module uses the principle of ion-selective electrodes to detect the concentrations of NH4+, K+, Na+, and Ca2+ ions and the pH value through different ion-selective membranes. The culture medium enters through inlet 21, passes through each detection unit, and flows out through outlet 22.

[0065] Each ion detection unit includes a specific ion-selective membrane and an internal filling chamber 19, with a silver chloride rod 18 serving as a reference electrode. When a specific ion passes through the corresponding selective membrane, a potential difference is generated, and the ion concentration can be calculated by measuring this potential difference.

[0066] This module can simultaneously detect multiple ion concentrations, with a detection range of NH4+ (0.1-100mM), K+ (0.1-150mM), Na+ (0.1-150mM), and Ca2+ (0.01-10mM), and a pH detection range of 2-12, providing comprehensive ion environment monitoring for biological reaction processes.

[0067] Example 4: System Integration and Intelligent Control

[0068] In this embodiment, the three functional modules are integrated into a complete system through fluid channels and electrical signal channels. The culture medium is sampled from the bioreactor by a peristaltic pump and then sequentially passed through the optical detection module, electrochemical sensor module, and ion detection module to achieve continuous monitoring of multiple parameters.

[0069] The system is equipped with an intelligent control unit that integrates data acquisition, signal processing, and feedback control functions. Based on the detection results, the control unit automatically adjusts parameters such as reactor temperature, pH value, stirring speed, and aeration rate to achieve precise control of the biological reaction process.

[0070] The system supports wireless data transmission and remote monitoring. Researchers can view the reactor status in real time, receive abnormal alarms, and remotely adjust operating parameters through a mobile app or computer, which greatly improves experimental efficiency and safety.

[0071] This invention enables real-time synchronous detection of OD value, more than 10 metabolites, 4 key ions and pH value, providing comprehensive data support for biological reaction processes. It is an important technological innovation in the fields of bioengineering, pharmaceutical industry and scientific research.

[0072] The working principle of this utility model:

[0073] Overall working principle

[0074] This invention relates to an intelligent bioreactor integrating multi-parameter biochemical analysis, an innovative device capable of real-time synchronous detection of multiple biochemical parameters. Its core working principle is based on three independent yet complementary detection modules: an optical detection module, an electrochemical sensor module, and an overflow cell biochemical detection module. These three modules are sequentially connected via fluid channels to form a complete detection flow path, enabling continuous monitoring of the same culture medium sample.

[0075] The system takes samples from the bioreactor using an automatic sampling device. The samples flow sequentially through three detection modules to obtain comprehensive data information. After being processed by the intelligent control unit, the system forms a comprehensive monitoring of the biological reaction process and can automatically adjust the reaction parameters based on the monitoring results to achieve intelligent control.

[0076] Working principle of the optical detection module: The optical detection module is based on the principle of optical density measurement and is mainly used to determine the concentration of microorganisms in the culture medium. Its working process is as follows:

[0077] Red light 2 emits red light of a specific wavelength, which is filtered by a red filter 3. The monochromatic light beam, focused by lens 1, passes through the culture medium sample in the first sample receiving cell 4. The microorganisms in the culture medium scatter and absorb the light beam, reducing the intensity of the light passing through the sample. The light transmitted through the sample is focused by lens 1 on the other side and received by receiver 5, which converts it into an electrical signal. By comparing the ratio of incident light intensity to transmitted light intensity, the optical density of the sample is calculated. The OD value is positively correlated with the concentration of microorganisms in the culture medium. By monitoring the OD value in real time, the growth status of microorganisms can be accurately grasped, providing a basis for the control of biological reaction processes.

[0078] Working principle of electrochemical sensor module:

[0079] The electrochemical sensor module, based on the principle of electrochemical analysis, is mainly used to detect various metabolites in culture media. Its working process is as follows:

[0080] The culture medium sample flows into the second sample receiving cell 11, where it contacts the electrode system. A platinum wire 8 serves as the working electrode, and a silver sheet 9 serves as the reference electrode, forming an electrochemical detection unit. Under an appropriate potential, metabolites (including glucose and lactic acid) in the culture medium undergo oxidation or reduction reactions on the surface of the working electrode. The current signal generated during the reaction is proportional to the metabolite concentration. After amplification and processing, the current signal is converted into a metabolite concentration value. This module can detect more than 10 metabolites, including glucose, lactic acid, ethanol, glycerol, and glutamate, providing comprehensive data support for metabolic monitoring in biological reactions. Different metabolites can be selectively detected by changing the electrode material or applying different potentials.

[0081] Working principle of the overflow pool biochemical detection module:

[0082] The overflow tank biochemical detection module, based on the principle of ion-selective electrodes, is mainly used to detect the concentration of various ions and pH value in the culture medium. Its working process is as follows:

[0083] The culture medium enters the overflow tank through the inlet 21 and flows sequentially through each ion detection unit. Each ion detection unit contains a specific ion-selective membrane and an internal filling chamber 19. When specific ions (NH4+, K+, Na+, Ca2+) pass through the corresponding selective membrane, a potential difference is generated across the membrane. The silver chloride rod 18 serves as a reference electrode, and the potential difference between it and the ion-selective electrode is logarithmically related to the concentration of the corresponding ion. After the potential difference signal is converted, the concentration values ​​of various ions are calculated. The pH detection unit measures the hydrogen ion concentration of the solution through a special pH-sensitive membrane to determine the pH value. After the detection is completed, the sample flows out from the outlet 22.

[0084] This module can simultaneously detect multiple ion concentrations and pH values, providing comprehensive information for monitoring the microbial culture environment.

[0085] Working principle of intelligent control system:

[0086] The intelligent control system serves as the hub connecting the three detection modules, responsible for data acquisition, processing, and feedback control. Its working principle is as follows:

[0087] It receives and processes electrical signals from three detection modules, converts them into corresponding parameter values, performs real-time data analysis to determine the state of the biological reaction, and automatically adjusts parameters such as reactor temperature, pH value, stirring speed, and aeration rate according to preset algorithms and control strategies. Through a wireless communication module, it realizes remote data transmission and monitoring, and combined with artificial intelligence algorithms, it continuously optimizes control parameters to improve the efficiency of the biological reaction.

[0088] System integration and data flow

[0089] The data flow and workflow of the entire system are integrated as follows:

[0090] The automated sampling system periodically samples the bioreactor. The samples first pass through an optical detection module to measure the OD value, then flow into an electrochemical sensor module to detect the concentration of various metabolites, and then into an overflow tank biochemical detection module to measure the concentration of multiple ions and pH value. The detection data from the three modules are simultaneously transmitted to the intelligent control unit. The intelligent control unit comprehensively analyzes the data, evaluates the state of the biological reaction, and automatically adjusts the operating parameters of the reactor based on the analysis results. The data is also transmitted to a remote monitoring terminal via a wireless network to achieve remote monitoring and data storage.

[0091] Through this integrated multi-parameter synchronous detection and intelligent control, this invention achieves comprehensive, real-time, and precise monitoring and control of the biological reaction process, greatly improving the efficiency of the biological reaction and the quality of the product, and providing important technical support for the fields of bioengineering, pharmaceutical industry and scientific research.

[0092] The above description is only a preferred embodiment of the present utility model, but the protection scope of the present utility model is not limited thereto. Any equivalent substitutions or changes made by those skilled in the art within the technical scope disclosed in the present utility model, based on the technical solution and the inventive concept of the present utility model, should be included within the protection scope of the present utility model.

Claims

1. An intelligent bioreactor integrating multi-parameter biochemical analysis functions, characterized in that, include: An optical detection module (100) is used to measure the optical density of the culture medium. The optical detection module (100) adopts the principle of transmission optical measurement and determines the optical density of the sample by measuring the intensity change of light before and after passing through the sample. An electrochemical sensor module (200) is used to detect the concentration of at least 10 metabolites in a culture medium. The electrochemical sensor module (200) adopts the principle of electrochemistry and detects the concentration of metabolites in a sample by measuring the change in potential difference between electrodes. The overflow pool biochemical detection module (300) is used to simultaneously detect the concentration of at least four ions, namely NH4+, K+, Na+, and Ca2+, and the pH value in the culture medium. The overflow pool biochemical detection module (300) adopts the principle of ion-selective electrode. The optical detection module (100), the electrochemical sensor module (200), and the overflow pool biochemical detection module (300) are connected in sequence through a fluid channel to form a complete detection flow path.

2. The intelligent bioreactor according to claim 1, characterized in that, The optical detection module (100) includes: Lenses (1) are set on both sides of the device; The red light (2) serves as a light source; The red filter (3) located in the optical path; The first sample receiving cell (4) is used to hold the sample to be tested; Receiver (5) for receiving optical signals.

3. The intelligent bioreactor according to claim 1, characterized in that, The electrochemical sensor module (200) includes: Overflow cap (6) for liquid overflow protection; A second sample receiving pool (11) for holding the sample to be tested; Platinum wire fixing shell for fixing electrodes (7); Platinum wire (8) serves as the working electrode; Silver sheet (9) serving as reference electrode; The first sealing ring (10) is used for system sealing.

4. The intelligent bioreactor according to claim 1, characterized in that, The overflow pool biochemical detection module (300) includes: Silver chloride rod holder (12) for fixing electrodes; The first shell (13), the second shell (14), the third shell (15), the fourth shell (16) and the fifth shell (17) constitute the external structure of the ion detection unit; Silver chloride rod (18) serves as a reference electrode; An ion-filled chamber (19) for holding the internal electrolyte; The second sealing ring (20) is used for system sealing; The inlet (21) and outlet (22) are used for sample entry and exit.

5. The intelligent bioreactor according to claim 4, characterized in that, The overflow pool biochemical detection module (300) includes multiple ion-selective electrodes for detecting the concentrations of NH4+, K+, Na+, Ca2+ ions and pH value.

6. The intelligent bioreactor according to claim 1, characterized in that, It also includes an intelligent control unit, which is electrically connected to the optical detection module (100), the electrochemical sensor module (200) and the overflow pool biochemical detection module (300) for collecting and processing detection data and automatically adjusting the operating parameters of the bioreactor based on the detection results.

7. The intelligent bioreactor according to claim 6, characterized in that, The intelligent control unit supports wireless data transmission and remote monitoring.

8. The intelligent bioreactor according to claim 1, characterized in that, The optical detection module uses red light with a wavelength of 630nm as the light source.

9. The intelligent bioreactor according to claim 1, characterized in that, The electrochemical sensor module can detect a variety of metabolites, including glucose, lactic acid, ethanol, glycerol, and glutamate.

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