A compound liquid for promoting apple sprouting and branching and a preparation and application method thereof

Abstract

The present application relates to the technical field of fruit tree branch development, and particularly relates to a compound liquid for promoting apple sprouting and branch development, and a preparation and application method thereof; the compound liquid is prepared from metabolites and plant growth regulators in different concentrations, the metabolites are sorbitol, hyperoside, genistein or melatonin, and the plant growth regulator is 6-benzylaminopurine; the application method is applied to the apple trees with bare trunks and lateral branches during the spring sprouting and growth period, and is applied at the initial sprouting stage of the apple trees, which can be during the period from mid-March to late April in spring every year, continuously for 2-3 weeks, 5 times, with 3-4 days interval, and can also be applied by using a brush or absorbent cotton within a range of 2 cm around the un-sprouting bud points of the apple bare trunks; the compound liquid with metabolites as the main functional component can effectively increase the sprouting and branch development amount of the apple trees, improve the tree shape, and increase the fruit setting rate.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of fruit tree sprouting, in particular to a compound liquid for promoting apple sprouting and a preparation and application method thereof. BACKGROUND

[0002] In recent years, most of the researches on fruit tree sprouting at home and abroad are concentrated on the use of plant growth regulators and other conventional hormones combined with artificial techniques to promote fruit tree sprouting, and the related researches only stay in the use of conventional hormones and the improvement of cultivation modes, etc., wherein the use of hormones brings problems such as the increase of production cost, the drug damage caused by improper use and the low yield and quality of agricultural products due to the fast growth of agricultural products. Although the traditional cultivation mode can meet people's pursuit of organic and healthy fruits, it consumes a lot of manpower and material resources.

[0003] The application patent with the publication number CN113615695A "A composition for improving yield and quality of fruit trees and application thereof" discloses that the mixed use of melatonin and compound plant regulators can improve the yield and quality of fruit trees. Meanwhile, the exogenous application of plant endogenous metabolites such as genistein, melatonin, sorbitol and hyperoside can promote the growth and development of plants.

[0004] On this basis, the current application methods are usually spraying, tree ring peeling or water and fertilizer control. The spraying method has a large application area and poor effect, and cannot accurately control the yield of fruit trees. The tree ring peeling method requires certain technical skills, otherwise it is easy to cause secondary damage to the tree body. As for the water and fertilizer control method, improper use of the agent can easily cause environmental pollution, and the control of water and fertilizer consumes more manpower and material resources. SUMMARY

[0005] In view of the problems in the prior art, the present application aims to provide a compound liquid for promoting apple sprouting and a preparation and application method thereof, which can improve the problems of drug damage, insufficient consumption of manpower and material resources caused by the traditional yield-increasing mode, and the problems of low efficiency and easy secondary damage to the tree body caused by the traditional application method, thereby increasing the number of branch sprouts, improving the tree shape and improving the yield.

[0006] To solve the above technical problems, the technical scheme provided by the present application is that the compound liquid for promoting apple sprouting is prepared by mixing metabolites and plant growth regulators in different concentrations, wherein the metabolites are sorbitol, hyperoside, genistein or melatonin, and the plant growth regulator is 6-benzylaminopurine.

[0007] The compound liquid with metabolites as the main functional component can effectively increase the sprouting and branching of apple trees, improve the tree shape and increase the fruit setting rate.

[0008] The preparation method of the complex liquid for promoting the sprouting and branching of apples, characterized in that 6-benzylaminopurine soluble liquid is used as a mother liquor, which is diluted to a final concentration of 0-400 mM to obtain a buffer solution, and a metabolite is compounded in the buffer solution to obtain the complex liquid.

[0009] Further, sorbitol with a final concentration of 0-100 mM is compounded in the 200 mM 6-benzylaminopurine buffer solution to obtain a complex sorbitol working solution.

[0010] Further, hyperoside with a final concentration of 0-2 mM is compounded in the 200 mM 6-benzylaminopurine buffer solution to obtain a complex hyperoside working solution.

[0011] Further, genistein with a final concentration of 0-4 mM is compounded in the 200 mM 6-benzylaminopurine buffer solution to obtain a complex genistein working solution.

[0012] Further, melatonin with a final concentration of 0-100 uM is compounded in the 200 mM 6-benzylaminopurine buffer solution to obtain a complex melatonin working solution.

[0013] The application further provides a use method of the complex liquid for promoting the sprouting and branching of apples, which is applied to apple trees with bare trunks and lateral branches during the spring sprouting and growth period, and is applied at the initial sprouting stage of the apple trees, can be applied from mid-March to late April in spring every year, is continuously maintained for 2-3 weeks, is applied 5 times, is applied once every 3-4 days, and is applied to a range of 2 cm around the un-sprouting bud points of the bare apple branches by using a brush or absorbent cotton.

[0014] When the apple trees with large areas of bare branches are still present after 3-4 times of application, i.e., about 14 days after the first application, the un-sprouting places are coated with the working solution and wrapped with absorbent cotton, and the absorbent cotton is maintained in the subsequent 1-2 times of application, so that the effect of the liquid medicine is increased and the branching rate is improved.

[0015] Further, the working solution needs to be prepared on site at the first time of application, and is stored in a 4°C refrigerator in the dark after being divided into small portions, and has a shelf life of one month. In addition, the frequency of outdoor application needs to be determined according to the weather conditions, and the application needs to be supplemented after the rain if it rains, and the application in a greenhouse does not need to be considered.

[0016] The above scheme can effectively increase the sprouting and branching amount of apple trees, improve the tree shape, and improve the fruit setting rate. BRIEF DESCRIPTION OF DRAWINGS

[0017] The accompanying drawings are included to provide a further understanding of the application, and constitute a part of the specification, illustrate the application together with the embodiments thereof, and are used to explain the application, and do not constitute a limitation on the application. In the drawings:

[0018] Figure 1 Statistical chart of sprouting rate for Example 1 of the present application;

[0019] Figure 2 Statistical chart of sprouting rate for Example 2 of the present application;

[0020] Figure 3 Statistical chart of sprouting rate for Example 3 of the present application;

[0021] Figure 4 Statistical chart of sprouting rate for Example 4 of the present application;

[0022] Figure 5 Statistical chart of sprouting rate for Example 5 of the present application;

[0023] Figure 6 Comparison result chart for Example 6 of the present application;

[0024] Figure 7 Schematic diagram of the application method of the present application. DETAILED DESCRIPTION

[0025] The preferred examples of the present application are described below in conjunction with the accompanying drawings, and it should be understood that the preferred examples described herein are only used to illustrate and explain the present application, and are not used to limit the present application.

[0026] As shown in Figure 7 The application method of the complex liquid for promoting apple sprouting and branching of the present application is applied to the apple trees with bare trunk and lateral branches during the spring budding and growing period, and is applied at the initial budding stage of the apple trees, which can be during the period from mid-March to late April in spring every year, continuously for 2-3 weeks, 5 times, with 3-4 days interval, using a brush or absorbent cotton to apply the working solution within 2 cm around the un-budded bud points of the apple bare branches.

[0027] When the working solution is applied for 3-4 times, i.e. about 14 days after the first application, if there are still apple trees with large area of bare branches, the absorbent cotton is wrapped around the un-budded branches after the working solution is applied, and the wrapping is kept in the subsequent 1-2 applications, which can increase the effect of the working solution and improve the branching rate.

[0028] The working solution needs to be prepared on site at the first application, and is stored in a 4°C refrigerator in the dark after being divided into small portions, with a shelf life of one month. In addition, the frequency of outdoor application needs to be determined according to the weather conditions, and if it rains, the application needs to be supplemented after the rain, and if it is applied in a greenhouse, it does not need to be considered.

[0029] Example 1:

[0030] Preparation of working solution: In this embodiment, the complex solution for promoting the germination and branching of apple trees is prepared by mixing metabolites and plant growth regulators at different concentrations. The 20% 6-benzylaminopurine soluble solution is used as the mother solution, which is diluted to a final concentration of 200 mM and 400 mM, and named as buffer-1 and buffer-2, respectively.

[0031] Application process: 18 apple trees with bare trunks and side branches in a greenhouse are randomly selected as the test objects. Among them, 6 are randomly selected as the control, i.e., without any treatment, 6 are randomly selected to be coated with buffer a, and 6 are randomly selected to be coated with buffer b. The coating is performed 5 times at intervals, and the period is 21 days.

[0032] Branching rate calculation method and formula screening: The number of ungerminated bud points on apple tree A in the first coating test is taken as the base (Ya). The total number of buds Xb is counted b days after each subsequent coating. The branching rate of apple tree A after b days is Xb / Ya*100%. The average number of ungerminated bud points on each test apple tree is about 70. The branching rate is counted 4, 7, 10, 14, 17, and 21 days after the first coating, as shown in Table 1. The results show that the bud emergence effect of buffer-1 is the most obvious. Figure 1

[0033] Example 2

[0034] Preparation of working solution: In this embodiment, the complex solution for promoting the germination and branching of apple trees is prepared by mixing metabolites and plant growth regulators at different concentrations. The 20% 6-benzylaminopurine soluble solution is used as the mother solution, which is diluted to a final concentration of 200 mM and 400 mM, and named as buffer-1 and buffer-2, respectively.

[0035] Application process: 18 apple trees with bare trunks and side branches in a greenhouse are randomly selected as the test objects. Among them, 6 are randomly selected as the control, i.e., without any treatment, 6 are randomly selected to be coated with buffer a, and 6 are randomly selected to be coated with buffer b. The coating is performed 5 times at intervals, and the period is 21 days.

[0036] Branching rate calculation method and formula screening: The number of ungerminated bud points on apple tree A in the first coating test is taken as the base (Ya). The total number of buds Xb is counted b days after each subsequent coating. The branching rate of apple tree A after b days is Xb / Ya*100%. The average number of ungerminated bud points on each test apple tree is about 70. The branching rate is counted 4, 7, 10, 14, 17, and 21 days after the first coating, as shown in Table 1. The results show that the bud emergence effect of buffer-1 is the most obvious. Figure 2

[0037] Example 3:​​

[0038] Working solution configuration: In this embodiment, the complex solution for promoting the germination and sprouting of apples is based on "buffer-1" in Example 1, and a working solution is prepared by compounding genistein. Genistein is diluted to a final concentration of 100 uM and 200 uM in the working solution based on 2 mM genistein stock solution, and is named genistein-1 and genistein-2 respectively.

[0039] Application process: 18 apple trees with bare trunks and side branches in the greenhouse are randomly selected as the test objects, of which 6 are randomly selected as the control group, i.e. without any treatment; 6 are randomly selected to be smeared with genistein-1, and 6 are randomly selected to be smeared with genistein-2. The smearing is performed 5 times at intervals, and the period is 21 days.

[0040] Sprouting rate calculation method and formula screening: The number of ungerminated bud points on apple tree A in the first smearing test is taken as the base (Ya), and the total number of buds Xb after b days of smearing is counted before each subsequent smearing, and the sprouting of apple tree A after b days is Xb / Ya*100%. The average number of ungerminated bud points on each test apple tree is about 70, and the sprouting rate is counted at 4, 7, 10, 14, 17 and 21 days after the first smearing, as shown in Table 1. Figure 3 The results show that the sprouting rate of genistein-1 and 2 is slightly higher than that of the control group.

[0041] Example 4:

[0042] Working solution configuration: In this embodiment, the complex solution for promoting the germination and sprouting of apples is based on "buffer-1" in Example 1, and a working solution is prepared by compounding genistein. Genistein is diluted to a final concentration of 100 uM and 200 uM in the working solution based on 2 mM genistein stock solution, and is named genistein-1 and genistein-2 respectively.

[0043] Application process: 18 apple trees with bare trunks and side branches in the greenhouse are randomly selected as the test objects, of which 6 are randomly selected as the control group, i.e. without any treatment; 6 are randomly selected to be smeared with genistein-1, and 6 are randomly selected to be smeared with genistein-2. The smearing is performed 5 times at intervals, and the period is 21 days.

[0044] Sprouting rate calculation method and formula screening: The number of ungerminated bud points on apple tree A in the first smearing test is taken as the base (Ya), and the total number of buds Xb after b days of smearing is counted before each subsequent smearing, and the sprouting of apple tree A after b days is Xb / Ya*100%. The average number of ungerminated bud points on each test apple tree is about 70, and the sprouting rate is counted at 4, 7, 10, 14, 17 and 21 days after the first smearing, as shown in Table 1. Figure 4As shown in the results, the application of genistein-2 significantly increased the sprouting rate between 10 days after the first application compared with the control group.

[0045] Example 5:

[0046] Working solution configuration: In this embodiment, the complex solution for promoting apple sprouting and branching is based on the "buffer-1" in Example 1, and melatonin is prepared into a working solution. Melatonin is diluted to 50uM, 100uM final concentration based on 10mM melatonin stock solution, and named as melatonin-1, melatonin-2 respectively.

[0047] Application process: 18 apple trees with bare trunks and lateral branches in the greenhouse were randomly selected, among which 6 were randomly selected as controls, i.e. without any treatment; 6 were randomly selected to apply melatonin-1, and 6 were randomly selected to apply melatonin-2. The application was repeated 5 times with an interval of 21 days.

[0048] Branching rate calculation method and formula screening: The number of ungerminated buds on apple tree A in the first application was taken as the base (Ya), and the total number of buds Xb was counted b days after the application. The branching rate of apple tree A after b days was Xb / Ya*100%. Figure 5 As shown in the results, the application of melatonin-2 significantly increased the sprouting rate between 14 days after the first application compared with the control group.

[0049] Example 6:

[0050] In this embodiment, an optimal formula is selected from the complex solutions tested in Examples 1-5. The complex solutions tested in Examples 1-5 include buffer-1, sorbitol-1, hyperoside-1, genistein-2 and melatonin-2, and the comparison results are as shown in Figure 6 As shown in the results, sorbitol-1 is the best among all the complex solutions, and the branching rate after 21 days can reach 80%.

[0051] Finally, it should be noted that the above description is only a preferred example of the present application and does not limit the present application. Although the present application has been described in detail with reference to the foregoing examples, those skilled in the art can still modify the technical solutions described in the foregoing examples or make equivalent substitutions for some technical features. Any modification, equivalent substitution, improvement, etc. made within the spirit and principles of the present application shall be included in the protection scope of the present application.

Claims

1. A compound liquid for promoting apple germination and branching, characterized in that: The compound liquid is prepared by a metabolite and a plant growth regulator according to different concentrations, wherein the metabolite is sorbitol and the plant growth regulator is 6-benzylaminopurine; The compound liquid is applied at the early stage of budding of apple trees for 2 to 3 consecutive weeks, 5 times at intervals of 3 to 4 days. When applying the solution 3-4 times, if there are still large areas of bare branches on the apple tree, apply the working solution to the unbudded area and wrap it with absorbent cotton, and keep it for the next 1-2 applications; 6-benzylaminopurine soluble solution is used as a mother solution, diluted to a final concentration of 200 mM to obtain a buffer solution, and a metabolite combination is compounded in the buffer solution to obtain a compound solution; Sorbitol was prepared in a 200 mM 6-benzylaminopurine buffer solution with a final concentration of 50 mM to obtain a prepared sorbitol working solution.

2. The compound liquid for promoting apple germination and branching according to claim 1, characterized in that: The working solution needs to be prepared freshly when applied for the first time and stored in aliquots away from light.

Images