Functional pig compound feed composition and use thereof

Abstract

The present invention provides a functional pig compound feed composition, a method for feeding lactating sows, and the use of the functional pig compound feed composition in increasing the average daily feed intake and / or milk production of lactating sows, or increasing the daily weight gain of piglets. The functional pig compound feed composition prepared with corn undersize fermentation products of the present invention can increase the average daily feed intake of lactating sows and increase the milk production, thereby ensuring the growth and development of piglets, thereby increasing the daily weight gain of piglets.

Description

Technical Field

[0002] The invention relates to the field of feed, and in particular to a functional pig compound feed composition and application thereof, and a method for feeding lactating sows. Background Art

[0004] In recent years, despite the continuous increase in global feed production, the shortage of feed resources has gradually become a bottleneck for the development of the world's feed industry. Therefore, on the one hand, feed raw materials are in short supply, and about 40% of the grain produced by cultivated land is used to produce feed. There are problems such as competition between people and animals for food, and we have to rely heavily on imports. The feed cost fluctuates significantly, which has become a bottleneck that has long restricted the development of my country's animal husbandry; on the other hand, due to the limitations of the existing feed processing and consumption methods, such as short time, low moisture, high energy consumption, and long shelf life, the room for improving the nutritional value of traditional feed is very limited; at the same time, a considerable amount of cheap food by-product resources are discarded in large quantities because they cannot be fully utilized by animals, resulting in resource waste and environmental pollution. It can be seen that the gap between feed supply and demand based on feed cost and nutritional value is still relatively large. It is currently believed that the development of bio-fermented feed can help alleviate the problem of scarce feed resources.

[0005] Corn is one of the major grain trade products, and it has an irreplaceable position in feed, food, chemical industry and other aspects. Generally speaking, corn in the three northeastern provinces of my country will go through the drying process after harvest to reduce its moisture content, so that the corn is not easy to mold and is easy to store. The moisture content of corn is usually around 30% at harvest, and the moisture content after drying is only 14%. During the entire screening process, corn is screened into oversize and undersize. Because the mycotoxin content of corn undersize exceeds the standard, the market value of these by-products cannot be best reflected when they are sold or processed separately. In addition, in the process of producing oils and fats with soybeans as raw materials, a large amount of soybean meal and soybean skin raw materials will be produced; in the process of flour processing, a large amount of bran raw materials will be produced; in the process of producing fructose syrup (F55 type) with corn as the main raw material, many by-products such as corn starch and sugar residue will be produced.

[0006] At present, the concept of feed safety has reached a new height both in the world and in China, and the production of antibiotic-free feed has become a trend in the animal husbandry industry. As a highly efficient, pollution-free, and residue-free natural health-care feed additive, biological feed has many biological functions, such as improving the palatability of feed, promoting the growth and development of pigs, increasing the digestibility and utilization rate of nutrients in feed, preventing digestive tract diseases, enhancing animal immunity, and reducing breeding costs. With the popularization of the application scope of fermented feed, it is urgent to master the law of the influence of fermented concentrated feed on the growth performance of growing and fattening pigs and to create and use safe, efficient, environmentally friendly, and antibiotic-free diets.

[0007] In pig production, pigs need different nutrients and dosages at different growth stages, so the ingredients and proportions of complete feeds must be adjusted accordingly at different growth stages. The feeds currently on the market are not only irrational in their complete feed formulas, but also not targeted at the growth of pigs at different growth stages, thus limiting the growth rate of pigs to a great extent.

[0008] If corn undersize can be effectively used for fermentation to produce biological feed for pig feeding, it can not only improve the utilization rate of feed resources, but also improve the economic value of corn products. Summary of the invention

[0010] To solve the above problems, the inventors prepared corn screenings fermented feed with reduced mycotoxin content and used it to formulate a functional pig feed composition, thereby being able to efficiently utilize corn screenings to produce feed, and unexpectedly found that the functional pig feed composition obtained in this way can significantly improve the production performance of lactating sows.

[0011] In a first aspect of the present invention, the present invention provides a functional pig compound feed composition, wherein the pig compound feed composition comprises a corn undersize fermented feed prepared by the following steps:

[0012] (1) mixing corn undersize, enzyme preparation and inorganic salt with corn, soybean meal, soybean hull, bran and sugar residue, wherein the enzyme preparation includes papain, and the inorganic salt includes ammonium salt, potassium salt and at least one selected from manganese salt and magnesium salt;

[0013] (2) inoculating a composite bacterial strain into the mixture obtained in step (1) to obtain a material to be fermented, wherein the composite bacterial strain includes Lactobacillus plantarum, Candida albicans and Bacillus subtilis;

[0014] (3) Fermenting the material to be fermented, and drying, cooling, and crushing the fermentation product to obtain the corn undersize fermented feed.

[0015] In another aspect, the present invention provides a method for feeding lactating sows, wherein the method comprises feeding lactating sows with the above-mentioned functional pig compound feed composition.

[0016] In another aspect, the present invention provides the use of the functional pig compound feed composition in increasing the average daily feed intake and / or milk production of lactating sows, or increasing the daily weight gain of piglets.

[0017] The results of simulated digestion data in vitro (dry matter digestibility, digestibility after defatting, energy value of enzymatic hydrolyzate) and digestion data in vivo (digestible energy) of pigs show that corn undercuts fermented feed can be used to prepare functional pig compound feed composition suitable for feeding pigs. The results unexpectedly found that the functional pig compound feed composition prepared with corn undercuts fermentation product of the present invention can increase the average daily feed intake of lactating sows and increase the milk production, thereby ensuring the growth and development of piglets, thereby increasing the daily weight gain of piglets. DETAILED DESCRIPTION

[0019] Next, exemplary embodiments of the present invention are described, but the protective scope of the present invention is not limited thereto.

[0020] Corn undersize refers to the material obtained by screening corn before and after drying in order to better ensure the preservation quality of corn before corn storage units are put into storage. High-moisture corn is first screened before drying, and the full kernels obtained after removing the undersize continue to be dried. The full kernels are screened for the second time after drying, and the corn broken kernels produced by drying can be stored in the warehouse after removing them. Therefore, a large amount of corn undersize can be produced during the corn drying process. Corn undersize includes broken corn kernels, shriveled kernels, bran, whiskers, core fragments, etc., of which the corn content is about 70wt%. Corn undersize contains rich nutrients such as crude starch and crude protein, which can provide energy and protein nutrition for animal feeding, and can also provide carbon and nitrogen sources for probiotic fermentation. However, since the mycotoxin content of corn undersize exceeds the standard, it cannot be directly used, resulting in a waste of resources.

[0021] Taking corn undercuts produced during corn processing as an example, an assessment of its nutritional content and mycotoxins was conducted. It was determined that the moisture content of the corn undercuts was 12.51wt%, the crude protein content was 8.81wt%, the crude fat content was 0.57wt%, the crude fiber content was 6.76wt%, the crude ash content was 2.53wt%, the crude starch content was 55.13wt%, and the reducing sugar content was 1.35wt%; the aflatoxin B1 content, the vomitoxin content, and the zearalenone content were 15.9ppb, 635.15ppb, and 3680ppb, respectively. Compared with the mycotoxin limit standards for corn in grain and food in my country (aflatoxin B1 content is ≤20ppb, vomitoxin content is ≤500ppb, and zearalenone content is ≤1000ppb), the contents of the two toxins, vomitoxin and zearalenone, in the corn undercuts exceeded the standard and the content of aflatoxin B1 was high, among which the zearalenone content exceeded the standard seriously, thus seriously limiting the resource utilization value of corn undercuts in the grain and food fields. At the same time, compared with the mycotoxin content limit of feed raw materials stipulated in the national standard "GB 13078-2017 Feed Hygiene Standard" (aflatoxin B1 ≤ 50 ppb, zearalenone (ZEN) ≤ ​​500 ppb, vomitoxin (DON) ≤ 5000 ppb), the zearalenone content in corn is also seriously exceeded, which also restricts its direct application as a feed raw material. Therefore, it is necessary to reduce the toxin content by processing corn screenings, thereby increasing the economic value of corn screenings and maximizing resource utilization.

[0022] In view of the above situation, the present invention has developed a functional pig compound feed composition, which degrades the content of mycotoxins in corn undersize by fermentation, thereby achieving better economic value and industrial value.

[0023] In some embodiments, the present invention provides a functional pig compound feed composition, wherein the pig compound feed composition comprises a corn undercut fermented feed prepared by the following steps:

[0024] (1) mixing corn undersize, enzyme preparation and inorganic salt with corn, soybean meal, soybean hulls, bran, sugar residue and molasses, wherein the enzyme preparation includes papain, and the inorganic salt includes ammonium salt, potassium salt, and at least one selected from manganese salt and magnesium salt;

[0025] (2) inoculating a composite bacterial strain into the mixture obtained in step (1) to obtain a material to be fermented, wherein the composite bacterial strain includes Lactobacillus plantarum, Candida albicans and Bacillus subtilis;

[0026] (3) Fermenting the material to be fermented, and drying, cooling, and crushing the fermentation product to obtain the corn undersize fermented feed.

[0027] In some preferred embodiments, the pig compound feed composition of the present invention further comprises: corn, short meal, rice bran, rice bran meal, soybean oil, soybean meal, fish meal, flaxseed, sumac fatty acid calcium, brown sugar, stone powder, calcium hydrogen phosphate, sodium chloride, choline chloride, L-lysine (sulfate), DL-methionine, L-threonine, potassium chloride, organic iron, phytase, Rayco 119 pig vitamin premix, A5702 pig trace element premix, ethoxyquin (60%) and mildew inhibitor.

[0028] The inventors first discovered that adding succinic acid calcium from sumac seeds to a pig compound feed composition helps to improve the nutritional value of the resulting pig compound feed in feeding lactating sows. As is known in the art, sumac seeds come from sumac trees and are mature seeds of the plant. It contains a large amount of natural oil and can be used to extract vegetable oil. Sumac oil is an edible vegetable oil and also a health oil with high medicinal value. It is made from the seeds of the plant sumac tree as the main raw material, and the vegetable oil is obtained after being squeezed and purified. It mostly exists in a solid form and has a natural aroma. It is more common in Yunnan, China. Sumac fatty acid calcium from sumac seeds is prepared by washing, decomposing, physically synthesizing, and drying sumac seed oil fatty acids and calcium oxide. By adding sumac fatty acid calcium from sumac seeds to a pig compound feed composition, the pig body can be strengthened to absorb calcium ions, enhance bone metabolism, increase milk production, prolong the peak of lactation, and improve feed absorption and utilization, which is beneficial to the growth of animals and their muscles.

[0029] In a further preferred embodiment, based on the total weight of the pig compound feed composition, the pig compound feed composition comprises: 40wt%-55wt% (49.3wt%) of the corn, 1wt%-10wt% (e.g. 5wt%) of the second meal, 1wt%-10wt% (e.g. 5wt%) of the rice bran, 0.5wt%-5.0wt% (e.g. 2.7wt%) of the rice bran meal, 0.1wt%-1.0wt% (e.g. 0.5wt%) of the soybean oil, 10wt%-20wt% (e.g. 16.2wt%) of the soybean meal, 0.5wt%-10wt% (e.g. 5wt%) of the rice bran meal, and 0.5wt%-10wt% (e.g. 2.7wt%) of the rice bran meal. t%-2wt% (e.g. 1.0wt%) of the sumac fatty acid calcium, 1.0wt%-3.0wt% (e.g. 2.0wt%) of the fish meal, 2.5wt%-4.5wt% (e.g. 3.5wt%) of the flax seed, 5wt%-20wt% (e.g. 10.0wt%) of the corn undersize fermented feed, 0.5wt%-2.5wt% (e.g. 1.5wt%) of the brown sugar, 0.1wt%-0.7wt% (e.g. 0.35wt%) of the stone powder, 0.6wt%-2wt% (e.g. 1.2wt%) of the calcium hydrogen phosphate, 0.1 wt%-1wt% (e.g. 0.59wt%) of the sodium chloride, 0.01wt%-0.5wt% (e.g. 0.15wt%) of the choline chloride, 0.1wt%-0.7wt% (e.g. 0.35wt%) of the L-lysine (sulfate), 0.01wt%-0.1wt% (e.g. 0.04wt%) of the DL-methionine, 0.02wt%-0.15wt% (e.g. 0.08wt%) of the L-threonine, 0.05wt%-0.5wt% (e.g. 0.15wt%) of the potassium chloride, 0.01wt%-0.15 wt% (e.g. 0.03wt%) of the organic iron, 0.01wt%-0.1wt% (e.g. 0.02wt%) of the phytase, 0.01wt%-0.15wt% (e.g. 0.035wt%) of the Raycus 119 pig vitamin premix, 0.1wt%-0.5wt% (e.g. 0.2wt%) of the A5702 pig trace element premix, 0.005wt%-0.05wt% (e.g. 0.015wt%) of the ethoxyquinoline (60%), and 0.01wt%-0.3wt% (e.g. 0.06wt%) of the antifungal agent.

[0030] In some embodiments, the content of the corn undersize is 10 wt %-50 wt % (eg, 20 wt %-40 wt %) relative to the total mass of the material to be fermented.

[0031] In some embodiments, the composite strain is composed of Lactobacillus plantarum 1.557 (CGMCC), Candida albicans 2.1180 (CGMCC) and Bacillus subtilis 1.1413 (CGMCC). In some preferred embodiments, the bacterial count ratio of Lactobacillus plantarum 1.557 (CGMCC), Candida albicans 2.1180 (CGMCC) and Bacillus subtilis 1.1413 (CGMCC) is (40-50):(10-70):(0.5-4), for example 45:(20-60):(2-3).

[0032] In some embodiments, the inoculation amount of the composite bacteria (in terms of volume-to-mass ratio) is 0.5%-10%, preferably 2%-5%.

[0033] In this article, the inorganic salt is preferably a water-soluble inorganic salt, so as to be more conducive to the fermentation. In some embodiments, the inorganic salt includes ammonium salt, potassium salt and manganese salt, or includes ammonium salt, potassium salt and magnesium salt. Preferably, the inorganic salt includes ammonium salt, potassium salt and manganese salt in a mass ratio of 2:2:1, or includes ammonium salt, potassium salt and magnesium salt in a mass ratio of 2:2:1. Wherein, when the inorganic salt includes manganese salt, a relatively better fermentation degradation effect can be achieved.

[0034] In some preferred embodiments, the ammonium salt is one or more selected from the following: ammonium chloride, ammonium sulfate, ammonium carbonate. In some preferred embodiments, the potassium salt is one or more selected from the following: potassium sulfate, potassium chloride, potassium dihydrogen phosphate, dipotassium hydrogen phosphate, potassium phosphate. In some preferred embodiments, the manganese salt is one or more selected from the following: manganese acetate, manganese sulfate, manganese chloride. In some preferred embodiments, the magnesium salt is one or more selected from the following: magnesium sulfate, magnesium chloride. In some more preferred embodiments, the inorganic salt includes ammonium sulfate, potassium dihydrogen phosphate and manganese sulfate; or includes ammonium sulfate, potassium dihydrogen phosphate and magnesium sulfate.

[0035] In some embodiments, the amount of the inorganic salt added is 0.3 wt%-1.0 wt%, for example 0.5 wt%, relative to the total mass of the material to be fermented.

[0036] In some embodiments, the amount of the enzyme preparation added is 0.3 wt%-1.0 wt%, for example 0.5 wt%, relative to the total mass of the material to be fermented.

[0037] In some embodiments, the fermentation is performed at 20°C to 50°C (eg, 25°C to 40°C) for 1-10 days (eg, 2-6 days).

[0038] In some embodiments, the present invention provides a method for feeding lactating sows, wherein the method comprises feeding lactating sows with the above-mentioned functional pig compound feed composition.

[0039] In some preferred embodiments, the method comprises: on the first day after farrowing, feeding 1.5kg-2.5kg (e.g. 1.9kg) of the pig compound feed composition to each lactating sow per day; on the second to fifth days after farrowing, feeding 0.2kg-1kg (e.g. 0.5kg) more of the pig compound feed composition to each lactating sow per day than the previous day; and after the sixth day after farrowing, the sows are allowed to eat freely.

[0040] In some embodiments, the present invention provides the use of the functional pig compound feed composition in increasing the average daily feed intake and / or milk production of lactating sows, or increasing the daily weight gain of piglets.

[0041] In some preferred embodiments, the average daily feed intake of the lactating sows can be increased by more than 10wt%. In other preferred embodiments, the daily weight gain of the piglets can be increased by more than 5wt%.

[0042] Exemplary embodiments of the present invention may be described by the following numbered paragraphs:

[0043] 1. A functional pig compound feed composition, wherein the pig compound feed composition comprises a corn undercut fermented feed prepared by the following steps:

[0044] (1) mixing corn undersize, enzyme preparation and inorganic salt with corn, soybean meal, soybean hulls, bran, sugar residue and molasses, wherein the enzyme preparation includes papain, and the inorganic salt includes ammonium salt, potassium salt, and at least one selected from manganese salt and magnesium salt;

[0045] (2) inoculating a composite bacterial strain into the mixture obtained in step (1) to obtain a material to be fermented, wherein the composite bacterial strain includes Lactobacillus plantarum, Candida albicans and Bacillus subtilis;

[0046] (3) Fermenting the material to be fermented, and drying, cooling, and crushing the fermentation product to obtain the corn undersize fermented feed.

[0047] 2. The pig compound feed composition as described in paragraph 1, wherein the pig compound feed composition further comprises: corn, bran meal, rice bran, rice bran meal, soybean oil, soybean meal, fish meal, flaxseed, sumac fatty acid calcium, brown sugar, stone powder, calcium hydrogen phosphate, sodium chloride, choline chloride, L-lysine (sulfate), DL-methionine, L-threonine, potassium chloride, organic iron, phytase, Rayco 119 pig vitamin premix, A5702 pig trace element premix, ethoxyquin (60%) and mildew inhibitor.

[0048] 3. The pig compound feed composition as described in paragraph 1 or 2, wherein, based on the total weight of the pig compound feed composition, the pig compound feed composition comprises: 40wt%-55wt% of the corn, 1wt%-10wt% of the second flour, 1wt%-10wt% of the rice bran, 0.5wt%-5.0wt% of the rice bran meal, 0.1wt%-1.0wt% of the soybean oil, 10wt%-20wt% of the soybean meal, 0.5wt%-2wt% of the sumac fatty acid calcium, 1.0wt%-3.0wt% of the fish meal, 2.5wt%-4.5wt% of the flax seed, 5wt%-20wt% of the corn undersize fermented feed, 0.5wt%-2.5wt% of the brown sugar, 0.1wt%-0.7wt% of the stone powder, 0.6wt%-2wt% of the phosphoric acid Calcium hydrogen, 0.1wt%-1wt% of the sodium chloride, 0.01wt%-0.5wt% of the choline chloride, 0.1wt%-0.7wt% of the L-lysine (sulfate), 0.01wt%-0.1wt% of the DL-methionine, 0.02wt%-0.15wt% of the L-threonine, 0.05wt%-0.5wt% of the potassium chloride, 0.01wt%-0 .15wt% of the organic iron, 0.01wt%-0.1wt% of the phytase, 0.01wt%-0.15wt% of the Raycus 119 pig vitamin premix, 0.1wt%-0.5wt% of the A5702 pig trace element premix, 0.005wt%-0.05wt% of the ethoxyquinoline (60%), and 0.01wt%-0.3wt% of the antifungal agent.

[0049] 4. The pig compound feed composition as described in any one of paragraphs 1 to 3, wherein the content of the corn undersize is 10 wt%-50 wt% relative to the total mass of the material to be fermented.

[0050] 5. The pig compound feed composition as described in any one of paragraphs 1 to 4, wherein the composite bacterial strains consist of Lactobacillus plantarum 1.557 (CGMCC), Candida albicans 2.1180 (CGMCC) and Bacillus subtilis 1.1413 (CGMCC).

[0051] 6. The pig compound feed composition as described in paragraph 5, wherein the bacterial count ratio of Lactobacillus plantarum 1.557 (CGMCC), Candida albicans 2.1180 (CGMCC) and Bacillus subtilis 1.1413 (CGMCC) is (40-50):(10-70):(0.5-4).

[0052] 7. The pig compound feed composition as described in any one of paragraphs 1 to 6, wherein the inoculation amount of the composite bacteria is 0.5%-10%.

[0053] 8. The pig compound feed composition as described in any of paragraphs 1 to 7, wherein the inorganic salt comprises an ammonium salt, a potassium salt and a manganese salt, or comprises an ammonium salt, a potassium salt and a magnesium salt.

[0054] 9. The pig compound feed composition as described in paragraph 8, wherein the inorganic salt comprises ammonium salt, potassium salt and manganese salt in a mass ratio of 2:2:1, or comprises ammonium salt, potassium salt and magnesium salt in a mass ratio of 2:2:1.

[0055] 10. The pig compound feed composition as described in paragraph 8 or 9, wherein the ammonium salt is one or more selected from the following: ammonium chloride, ammonium sulfate, ammonium carbonate;

[0056] The potassium salt is one or more selected from the following: potassium sulfate, potassium chloride, potassium dihydrogen phosphate, dipotassium hydrogen phosphate, potassium phosphate;

[0057] The manganese salt is one or more selected from the following: manganese acetate, manganese sulfate, manganese chloride;

[0058] The magnesium salt is one or more selected from the following: magnesium sulfate and magnesium chloride.

[0059] 11. The pig compound feed composition as described in any of paragraphs 8 to 10, wherein the inorganic salt comprises ammonium sulfate, potassium dihydrogen phosphate and manganese sulfate; or comprises ammonium sulfate, potassium dihydrogen phosphate and magnesium sulfate.

[0060] 12. The pig compound feed composition as described in any one of paragraphs 1 to 11, wherein the amount of the inorganic salt added is 0.3 wt%-1.0 wt% relative to the total mass of the material to be fermented.

[0061] 13. The pig compound feed composition as described in any one of paragraphs 1 to 12, wherein the enzyme preparation is added in an amount of 0.3 wt% to 1.0 wt% relative to the total mass of the material to be fermented.

[0062] 14. The pig compound feed composition as described in any of paragraphs 1 to 13, wherein the fermentation is carried out at 20°C to 50°C for 1 to 10 days.

[0063] 15. A method for feeding lactating sows, wherein the method comprises feeding lactating sows with the functional pig compound feed composition described in any one of paragraphs 1 to 14.

[0064] 16. The method as described in paragraph 15, wherein the method comprises: on the first day after farrowing, feeding each lactating sow with 1.5kg-2.5kg of the pig compound feed composition per day; on the second to fifth days after farrowing, feeding each lactating sow with 0.2kg-1kg more of the pig compound feed composition per day than the previous day; and after the sixth day after farrowing, the sows are allowed to eat freely.

[0065] 17. Use of the functional pig compound feed composition described in any one of paragraphs 1 to 14 for increasing the average daily feed intake and / or milk production of lactating sows, or increasing the daily weight gain of piglets.

[0066] 18. The use as described in paragraph 17, wherein the average daily feed intake of the lactating sow is increased by more than 10wt%.

[0067] 19. The use as described in paragraph 17 or 18, wherein the daily weight gain of the piglet is increased by more than 5wt%.

[0068] Example

[0069] Next, the present invention is further described in detail by examples, but the present invention is not limited to these examples. Unless otherwise specified, the raw materials, reagents, instruments, etc. used in the following examples are conventional commercially available raw materials, reagents, instruments.

[0070] The sources of corn undersize materials used in the following examples and the methods for determining various indicators, cultivating bacteria and counting are as follows:

[0071]

[0072] Collect screenings from corn processing at COFCO Trading.

[0073] 2. Evaluation of mycotoxin indexes in corn screenings

[0074] 1) Conventional nutritional indicators include: moisture, crude protein, crude fat, crude fiber, crude ash, crude starch, and reducing sugar.

[0075] Conventional index determination method: In the following examples, moisture is determined by "GB / T6435 Determination of moisture and other volatile substances in feed", crude protein on a dry basis is determined by "GBT 6432 Determination of crude protein in feed", crude fat on a dry basis is determined by "GBT 6433 Determination of crude fat in feed", crude fiber on a dry basis is determined by "GBT6434-2007 Determination of crude fiber in feed", crude ash on a dry basis is determined by "GBT 6438-2007 Determination of crude ash in feed", crude starch on a dry basis is determined by "GB / T 5009.9-2008 Determination of starch in food", and reducing sugar on a dry basis is determined by "GB / T 5513-2008 Grain and oil inspection - Determination of reducing sugar and non-reducing sugar in grain".

[0076] Table 1 Test results of conventional nutrient content in corn sieves

[0077]

[0078] 2) Mycotoxins mainly determine the content of aflatoxin B1, vomitoxin and zearalenone in the sieve.

[0079] Mycotoxin determination method: In the following examples, samples were tested for aflatoxin B1, zearalenone (F2 toxin), and vomitoxin using ELISA enzyme-linked immunosorbent assay kits. The determination of aflatoxin B1 was based on the determination method of aflatoxin B1 (Order No.: COKAQ8000) in the Romer Labs Singapore Pte. Ltd. kit, the determination of zearalenone was based on the determination method of zearalenone (Order No.: COKAQ5100) in the Romer Labs Singapore Pte. Ltd. kit, and the determination of vomitoxin was based on the determination method of vomitoxin (Order No.: COKAQ4000) in the Romer Labs Singapore Pte. Ltd. kit. Others were based on GB / T 6682-1992 Specifications and Test Methods for Water Use in Analytical Laboratories and GB / T 14699.1, a feed sampling standard.

[0080] Table 2 Results of toxin content detection in corn sieves

[0081] Corn Screenings Detection Technology Aflatoxin B1 15.90ppb Colloidal gold test paper method Zearalenone 635.15ppb ELISA Deoxynivalenol 3680.00ppb ELISA

[0082] The national standard "GB 13078-2017 Feed Hygiene Standard" stipulates the following limits for the mycotoxin content of feed raw materials: aflatoxin B1 ≤ 50 ppb, zearalenone (ZEN) ≤ ​​500 ppb, and vomitoxin (DON) ≤ 5000 ppb. Therefore, untreated corn screenings are not suitable as feed raw materials.

[0083] 3. Preparation of shake flask seeds:

[0084] Shake flask seed culture process: prepare the following culture medium, sterilize at 121℃ for 20min, cool the bacterial suspension and inoculate it into the culture medium, culture under the corresponding control parameter conditions, measure OD (wavelength 600nm, cuvette width 1cm) and pH value during the culture process, and when the termination parameters are reached, the shake flask seed culture is completed.

[0085] Lactobacillus:

[0086] Culture medium: 48 g of MRS broth medium in 1000 ml of distilled water, adjusted to pH 6.2 ± 0.1.

[0087] Control parameters: 30°C, anaerobic culture under static conditions.

[0088] Termination parameter: OD ≥ 0.250.

[0089] Yeast:

[0090] Culture medium: 19 g peptone, 19 g glucose, 9.5 g yeast extract, and distilled water to make up to 1000 ml.

[0091] Control parameters: 30℃, 200r / min.

[0092] Termination parameter: OD ≥ 0.320.

[0093] Bacillus:

[0094] Culture medium: 5g yeast powder, 10g peptone, 5g glucose, 10g sodium chloride, 1000ml water. Use 1mol / L NaOH solution to adjust the pH value to alkaline.

[0095] Control parameters: 35℃, 160r / min.

[0096] Termination parameter: OD ≥ 0.120.

[0097] 4. Plate count method:

[0098] The bacterial content was determined by the plate count method. The preparation methods of the plate culture medium used for lactic acid bacteria, yeast, and bacillus were as follows:

[0099] Lactic acid bacteria plate culture medium: weigh 55.2 g / L of MRS broth medium and 10.14 g / L of agar, add distilled water to make up the volume, adjust the pH value to 6.2, sterilize at 120°C for 20 min, make a plate culture medium, and culture for 24 h to ensure no contamination before use.

[0100] Yeast plate culture medium: weigh 10 g / L yeast powder, 20 g / L peptone, 20 g / L glucose, and 14 g / L agar, add distilled water to make up to volume, adjust the pH value to 6.0, sterilize at 120°C for 20 min, make a plate culture medium, and culture for 24 h to ensure no contamination.

[0101] Bacillus plate culture medium: weigh 5 g / L yeast powder, 10 g / L peptone, 5 g / L glucose, 10 g / L sodium chloride, and 20 g / L agar, add distilled water to make up to volume, adjust the pH value to 7.2, sterilize at 120°C for 20 min, make a plate culture medium, and culture for 24 h to ensure no pollution.

[0102] The bacterial content is determined by the plate count method. Weigh 5.0g of the material into 45g of sterile water, place it on a shaker for culture (temperature 32°C, speed 120rpm / min, time 40-50min), then take 0.1mL of culture solution to 0.9mL of sterile water and shake well, dilute and spread it on the plate culture medium of the corresponding strain, and place it in a constant temperature box at 30°C for 48h. Counting method: The number of colonies at a dilution is 30-300 colonies. The total number of bacteria in the sample is the number of colonies at the dilution divided by the volume of the dilution solution used for the plate coating (0.1mL) and multiplied by the dilution multiple.

[0103] Example 1 Preparation of high-end microbial fermentation concentrated feed (drying) using corn undersize

[0104] In this embodiment, corn undersize (aflatoxin B1 content of 15.9 ppb, zearalenone content of 635.15 ppb, vomitoxin content of 3680 ppb), corn, bran, starch sugar residue were purchased from COFCO (Chengdu) Cereals and Oils Industry Co., Ltd., soybean meal and bean skin were purchased from COFCO Oils (Heze) Co., Ltd., beet molasses was purchased from COFCO Sugar Co., Ltd., papain (≥800000 U / g) was purchased from Beijing Solebow Technology Co., Ltd., and various inorganic salts were purchased from Sinopharm Chemical Reagent Co., Ltd.

[0105] The first-grade seed solution of Lactobacillus plantarum 1.557 (purchased from CGMCC), Candida albicans 2.1180 (purchased from CGMCC) and Bacillus subtilis 1.1413 (purchased from CGMCC) obtained according to the description of the "Preparation method of shake flask seeds" section above (wherein the contents of Lactobacillus plantarum 1.557, Candida albicans 2.1180 and Bacillus subtilis 1.1413 were 6×109 cfu / mL, 8×10 9 cfu / mL and 2×10 8 cfu / mL) in a volume ratio of 3:3:4 to obtain a composite bacterial strain mixed solution. Then, 0.5 parts by weight of papain, 0.5 parts by weight of inorganic salt (composed of (NH4)2SO4, KH2PO4 and MnSO4 in a mass ratio of 2:2:1), 30 parts by weight of corn undersize, 26 parts by weight of corn, 30 parts by weight of soybean meal, 2 parts by weight of bean skin, 2 parts by weight of bran, and 6 parts by weight of starch residue were mixed and broken up, and then mixed with 1 part by weight of beet molasses, and 2 parts by weight of the composite bacterial strain mixed solution were added thereto in an inoculation amount of 2% (volume-to-weight ratio) to obtain a material to be fermented.

[0106] The material to be fermented is placed in a breathing bag (an airtight packaging bag provided with a one-way exhaust valve through which gas can only be discharged from the bag to the outside of the bag) with a volume ratio of the material in the breathing bag to the gas sealed in the bag of 10:1. The fermentation is carried out for 96 hours under the following conditions: the oxygen content is 4 vol%, the temperature is 30°C, and the initial pH is 7.

[0107] The product (wet material) obtained by the above fermentation is dried (boiling drying, inlet air temperature 140°C), naturally cooled, crushed and passed through a 20-mesh sieve to obtain a high-end corn undersize fermented concentrated feed (drying).

[0108] In the effect examples section below, the corn undersize fermented concentrated feed obtained above (also referred to as "fermented concentrated feed" or "fermented feed of Example 1") was evaluated.

[0109] Example 2 Preparation of functional compound feed composition

[0110] Rhus tree seed fatty acid calcium is provided by COFCO Nutrition & Health Research Institute Co., Ltd. and is made by washing, decomposing, physically synthesizing and drying Rhus tree seed oil fatty acids and calcium oxide.

[0111] Corn (first grade), second-grade flour, rice bran and rice bran meal (all purchased from COFCO (Chengdu) Grain and Oil Industry Co., Ltd.), soybean oil and soybean meal (all purchased from COFCO Oils and Fats (Heze) Co., Ltd.), fish meal (purchased from Tianjin Ronghai International Trade Co., Ltd.), flaxseed (purchased from Shaanxi Xintianyu Biotechnology Co., Ltd.), sumac fatty acid calcium, brown sugar (purchased from COFCO Sugar Co., Ltd.), stone powder (purchased from Shijiazhuang Weijia Mineral Products Co., Ltd.), calcium hydrogen phosphate (purchased from Shandong Hongshengda Chemical Co., Ltd.), sodium chloride (purchased from Jinan Ruilin Chemical Co., Ltd.), choline chloride (purchased from Shandong Siyang Biotechnology Co., Ltd.), L-lysine (sulfate) (purchased from Shandong Qilu Chemical Technology Co., Ltd.), DL-methionine (purchased from Xi'an Lavia Biotechnology Co., Ltd.), L-threonine (purchased from Jinan Qiyue Chemical Technology Co., Ltd.), potassium chloride (purchased from Jiangsu Kelunduo Food Ingredients Co., Ltd.), organic iron (purchased from Wuhan Shuer Biotechnology Co., Ltd.), phytase (purchased from Jiangsu Yinong Biological Co., Ltd.), Ruike 119 pig vitamin premix (purchased from COFCO (Beijing) Feed Technology Co., Ltd.), A5702 pig trace element premix (purchased from COFCO (Beijing) Feed Technology Co., Ltd.), ethoxyquinoline (60%) (purchased from Yixing Tianshi Feed Co., Ltd.) and mildew inhibitor (dimethyl fumarate, CAS No. 624-49-7, purchased from Shandong Suihua Biotechnology Co., Ltd.), selectively add the high-end corn undersize fermented concentrated feed (dried) prepared in Example 1 to prepare two different functional pig compound feed compositions. Specifically, two different types of pig compound feed compositions were prepared according to the formula in Table 3 as the lactating sow diets used in the following effect examples: C type lactating sow diet and C high-end lactating sow diet.

[0112] Table 3 Formula of lactating sow diet (weight / wt%)

[0113]

[0114] Effect Example 1 Detection of Microbial Fermentation Feed from Corn Screenings

[0115] The corn undersize fermented concentrated feed prepared in Example 1 was tested according to national standards, wherein the moisture content detection standard was based on GB / T 6435-2014, the crude protein content detection standard was based on GB / T 6432-2018, the crude fat content detection standard was based on GB / T 6433-2006, the crude fiber content detection standard was based on GB / T 6434-2006, the crude ash content detection standard was based on GB / T 6438-2007, the acid-soluble protein content detection standard was based on GB / T 22492-2008, the calcium content detection standard was based on GB / T 6436-2002, the phosphorus content detection standard was based on GB / T 6437-2002, and the lactic acid content detection standard was based on GB / T SXA03-FB-016.

[0116] Determination of mycotoxins in hazardous materials: Samples were tested for aflatoxin B1, zearalenone (F2 toxin) and vomitoxin using ELISA kits. The determination of aflatoxin B1 was carried out in accordance with the determination method of aflatoxin B1 (Order No.: COKAQ8000) in the Romer LabsSingapore Pte. Ltd. kit, the determination of zearalenone was carried out in accordance with the determination method of zearalenone (Order No.: COKAQ5100) in the Romer Labs Singapore Pte. Ltd. kit, and the determination of vomitoxin was carried out in accordance with the determination method of vomitoxin (Order No.: COKAQ4000) in the Romer Labs Singapore Pte. Ltd. kit. Others were carried out in accordance with GB / T6682-1992 Specifications and Test Methods for Water in Analytical Laboratories and Feed Sampling Standard GB / T 14699.1.

[0117] Determination of heavy metal and fluorine content in hazardous substances: The lead content detection standard is based on GB / T 13080-2018, the arsenic content detection standard is based on GB / T 13079-2006, the mercury content detection standard is based on GB / T 13081-2006, the chromium content detection standard is based on GB / T 13088-2006, and the fluorine content detection standard is based on GB / T 13083-2018.

[0118] The samples were tested for the content of 18 kinds of amino acids in feed using amino acid analyzer and high performance liquid chromatography. The 18 kinds of amino acids were determined on a dry basis according to GB / T 18246-2019 Determination of Amino Acids in Feed.

[0119] Results and Analysis:

[0120] Table 4 Nutritional composition of corn undersize fermented feed (wt%)

[0121] Moisture Crude Protein Crude fat Crude Fiber Coarse ash Amino Acids Acid soluble protein calcium phosphorus lactic acid Fermented concentrated feed 6.36 27.12 3.97 3.83 6.19 24.98 12.15 0.32 0.37 3.86

[0122] Table 5 Test results of mycotoxins in feed raw materials (before fermentation) and corn undersize fermented feed (after fermentation)

[0123] Mycotoxin content before fermentation Mycotoxin content after fermentation Degradation rate Aflatoxin B1 7.91ppb 0.87ppb 89.00% Zearalenone 508.86ppb 283.13ppb 44.36% Deoxynivalenol 1.75ppb 0.90ppb 48.62%

[0124] Table 6 Heavy metal and fluoride content of corn undersize fermented feed (mg / kg)

[0125]

[0126] Table 7 Amino acid detection of corn undersize fermented feed (wt%)

[0127] Aspartic acid Threonine Serine Glutamate Proline Glycine Alanine Cystine Valine 2.78% 1.02% 1.26% 4.36% 1.62% 1.08% 1.43% 0.36% 1.37% Methionine Isoleucine Leucine Tyrosine Phenylalanine Histidine Lysine Arginine Tryptophan 0.48% 1.17% 2.30% 0.82% 1.18% 0.62% 1.35% 1.51% 0.27%

[0128] As can be seen from the table above, after fermentation, the aflatoxin B1 content in the corn undercuts fermented concentrated feed is 0.87 ppb, the zearalenone content is 283.13 ppb, and the vomitoxin content is 0.90 ppm; therefore, the degradation rate of aflatoxin B1 in the feed obtained by the synergistic fermentation of bacteria, enzymes and inorganic salts is 89.00%, the degradation rate of zearalenone is 44.36%, and the degradation rate of vomitoxin is 48.62%, and the comprehensive degradation effect of mycotoxins is significant. The corn undercuts fermented concentrated feed meets the relevant standards for toxin quality control in feed, so it can be used in pig compound feed.

[0129] The total amount of amino acids in the fermented concentrated feed of corn undersize prepared in Example 1 is 24.98wt%, the total amount of amino acids in the high-quality type (drying) is 18.02wt%, and the amino acid content is very balanced.

[0130] Effect Example 2: In vitro (monogastric animal bionic digestive system) evaluation test of corn undersize fermented concentrated feed

[0131] The digestion and absorption characteristics of feed directly reflect the utilization value of feed raw materials and the ability of animals to utilize feed raw materials. The digestion characteristics of feed are mainly evaluated through digestion experiments. By using in vitro analytical enzymatic hydrolysis, protease is used to simulate the digestion process of feed by animals, so as to evaluate the fermented feed of corn undersize prepared in Example 1 in vitro.

[0132] The digestibility of feed raw materials or complete feed is determined by using a monogastric animal bionic digestion instrument and digestive enzyme hydrolysis method.

[0133] According to the manual of the monogastric animal bionic digestion system, stomach buffer, small intestine buffer, large intestine buffer, stomach digestion fluid, small intestine digestion fluid, and large intestine digestion fluid were prepared. Among them, pepsin, trypsin, chymotrypsin, and large intestine enzymes were purchased from Hunan Zhongben Zhinong Technology.

[0134] Put the gastric buffer, small intestine buffer and large intestine buffer into the special bottles provided with the instrument respectively, and place them at the designated positions of the instrument, and then connect the system pipes to the buffer bottles.

[0135] Weigh 2 g of the feed sample prepared in Example 1 into a 10 ml glass test tube. Use a dialysis bag to simulate the digestive tract, transfer the sample in the glass test tube into it, rinse the glass test tube with 20 ml of gastric digestive fluid, and transfer all the rinsing fluid into the dialysis bag. Place the dialysis bag on a digestive tract stand.

[0136] According to the operation manual of the bionic digestion system, enter the gastric digestion stage, small intestine digestion stage and large intestine digestion stage in sequence. After digestion, pour the undigested residue in the dialysis bag into a 90mm culture dish with constant weight, then cut the dialysis bag longitudinally, and rinse the residue remaining on the dialysis bag into the culture dish with deionized water. Bake the culture dish containing the digestion residue at 65°C until there is no water mark, then bake it at 105°C to constant weight, and weigh it. Scrape off all the digestion residue in the culture dish, transfer it to a glass sand crucible with a known absolute dry weight, and weigh it after constant weight at 105°C for 2h. Rinse the residue with anhydrous ethanol 3 times under suction filtration conditions, 30ml each time. After the anhydrous ethanol is basically evaporated, place the glass crucible with defatted undigested residue at 105°C for 2h.

[0137] The results are shown in Table 9 below.

[0138] Effect Example 3 Animal Energy Metabolism Test Results

[0139] The absorption and utilization of exogenous nutrients by animals is currently mainly completed through animal feeding experiments, and the absorption performance of feed is evaluated by utilizing digestible energy.

[0140] Twelve healthy castrated three-way crossbred pigs (Dor × Long × Long) with an initial body weight of about 55 kg were selected and housed in stainless steel metabolic cages (1.20 × 0.70 × 0.96 m 3 The experimental pigs were randomly divided into one experimental diet group and one basal diet group, and each treatment was set up with 6 replicates, and each replicate had 1 pig.

[0141] This experiment was conducted in the pig metabolism laboratory of Fengning Animal Experiment Base (Fengning, Hebei). Each experimental period was 7 days, with the first 5 days as an adaptation period and feces and urine collected by the full feces collection method on days 6 to 7. The basal diet of this experiment consisted of corn (76.00%), dehulled soybean meal (21.00%) and premix (3.00%). The composition of the basal diet and experimental diet is shown in Table 8.

[0142] Table 8 Feed formula of experimental diet and basal diet (wt%)

[0143]

[0144] In this experiment, the total amount of feed for the experimental diet group and the basal diet group was 4wt% of the pig's body weight (Adeola, 2001); equal amounts were fed twice a day at 8:00 and 17:00. During the entire experimental period, the pigs were allowed to drink water freely. During the collection period, the feed intake, food refusal and feed spillage of each pig were accurately recorded. During the entire experimental period, the pig house environment was kept clean and sanitary, and the room temperature was controlled at 22 ± 2℃.

[0145] At the beginning of the collection period, place the manure collecting pan under the pig cage slats to ensure that all the manure discharged by the pig falls into the manure collecting pan. If a small amount of manure falls on the slats, it must be cleaned up completely. Collect the manure discharged by each experimental pig every day, put the manure of each pig in each period into the corresponding plastic bag, and store it in a -20℃ refrigerator immediately after each collection. After the end of the test period, all the manure collected for 5 days was thawed at room temperature, mixed evenly, and weighed. Take 2 300 g small samples from the mixed manure sample of each pig and place them evenly on the baking tray, spray 15 mL of 6 N HCl on the surface of the pig manure for nitrogen fixation, and then put it in a 65℃ oven for 72 hours to air dry state, and rehydrate to constant weight after 24 hours. The dried manure samples were retained according to the pig number, crushed through a 40-mesh sieve, and bagged for analysis.

[0146] When collecting urine samples during the experimental period, place a urine bucket containing 10 mL of 6 N HCl under the metabolic cage to collect urine samples from each pig every day. Place gauze above the urine bucket to prevent feed and feces from falling into the urine samples. Collect urine samples from each pig every morning during the experimental period. Accurately measure the total urine volume of each pig every day, mix it evenly, measure 1wt% of the total urine volume and transfer it to the urine collection bottle, and freeze it in time. After the collection period is over, thaw the 5-day urine sample and transfer it to a bucket to mix it evenly, then take part of the urine sample and put it into a 50 mL centrifuge tube. The urine sample is frozen and stored for testing and analysis.

[0147] The test results are shown in Table 9 below.

[0148] Table 9 In vitro simulated digestion and in vivo energy metabolism of pigs

[0149]

[0150] In the in vitro digestion simulation experiment of pigs, the energy value of the enzymatic hydrolyzate of the fermented concentrated feed of corn undersize prepared in Example 1 was 14.8209 MJ / Kg.

[0151] In the digestion experiment in pigs, the digestible energy value of the fermented concentrated feed of corn undercuts prepared in Example 1 was 3172 J / g. In this article, digestible energy refers to the energy contained in the feed that is digested and absorbed, that is, digestible energy = total energy of feed - feces energy - gas energy such as methane that cannot be used by animals. Therefore, a high digestible energy value means that the energy that can be digested and absorbed in the feed is higher. The digestion and absorption characteristics of the feed determine the efficiency and degree of utilization of the feed by the animal. As can be seen from the above table, the fermented concentrated feed of corn undercuts described in the present invention has a high digestible energy value.

[0152] In the present invention, the high-end corn undercut fermented feed (drying) of Example 1 showed good results in terms of simulated digestion data (dry matter digestibility, digestibility after defatting, energy value of enzymatic hydrolyzate) in vitro and digestion data (digestible energy) in vivo. Therefore, the corn undercut fermented concentrated feed prepared by the present invention can be used to prepare a functional pig compound feed composition, thereby improving the utilization value of corn undercuts.

[0153] Effect Example 4 Feeding Experiment on Production Performance of Lactating Sows

[0154] In order to explore the effects of the two functional pig compound feed compositions prepared in Example 2 on the production performance of lactating sows, this experimental study was carried out to determine the effects of fermented corn undersize feed added to the compound feed composition on the production performance of lactating sows.

[0155] 1. Experimental Design

[0156] Twenty healthy sows with 3-5 parities and close due dates were selected and randomly divided into a control group and a test group, with 10 replicates in each group and 1 sow in each replicate. The sows in the control group were fed the C-type lactating sow diet prepared in Example 2, and the sows in the test group were fed the C-type lactating sow diet prepared in Example 2 (supplemented with 10wt% corn undersize fermented feed).

[0157] 2. Feeding and management

[0158] The sows in each group were transferred to the farrowing house 3 days before the expected date of delivery. The farrowing room was equipped with high beds and slatted floors. The pens were well ventilated, kept clean and dry, and conventional feeding management was adopted. No feed was given on the day of farrowing (recorded as day 0 of the experiment), and the sows were fed the corresponding feed according to the group after farrowing. Restricted feeding was adopted for the first 5 days after farrowing, with 1.9 kg / day on the first day, and then increased by 0.5 kg per day. Free feeding and free drinking water began on the 6th day.

[0159] 3. Index determination and methods

[0160] 3.1 Lactating sow production performance

[0161] The birth weight of piglets was weighed immediately after farrowing, and the litter size (number of piglets in each litter), litter weight (total weight of piglets in each litter), and average head weight at birth were calculated. The individual weight of piglets in each litter was weighed at 08:00 on the morning of the 21st day of the experiment, the number of piglets in each litter was recorded, and the average head weight, litter weight, litter weight gain, and average daily weight gain on the 21st day were calculated.

[0162] 3.2 Feed intake of lactating sows

[0163] Record the daily feed intake and remaining feed amount of the sows during lactation, and calculate the average daily feed intake of the sows.

[0164] 4. Experimental Procedure

[0165] The experiment was conducted at the Fengning Animal Experimental Base in Chengde City, Hebei Province, China Agricultural University, Ministry of Agriculture Feed Industry Center. The sows were transferred to the farrowing house 3 days before the due date, and the difference in due date of the same farrowing house was less than 10 days. The farrowing house has 3 rows of aisles, tail-to-tail design. One row of 10 sows was fed with C-type lactating sow diet (control lactating sow feed), and another row of 10 sows was fed with C-type lactating sow diet (supplemented with 10wt% corn undersize fermented feed). The technicians fed and recorded the experimental data on site. One sow in the experimental group was eliminated midway due to an epidemic.

[0166] The results of the experiment are shown in Table 10 below.

[0167] Table 10 Experimental results of lactating sow production performance

[0168]

[0169] As can be seen from Table 10, the lactating sows in the experimental group were fed with the C high-end lactating sow diet (supplemented with 10wt% corn undersize fermented feed). During the 21-day lactation period, the average daily feed intake of each sow in this group was 5.79 kg, which was 0.61 kg more than the average daily feed intake of sows in the control group (5.18 kg).

[0170] After 21 days of lactation, the lactating sows in the experimental group were fed the C high-end lactating sow diet (supplemented with 10wt% fermented corn undersize feed), and their piglets had an average daily weight gain of 248.20 g / day, which was 16.35 g higher than the average daily weight gain of 231.85 g / day of piglets produced by sows in the control group.

[0171] The results of the above experiments show that the use of corn undercuts fermented feed to prepare a functional pig feed composition can increase the average daily feed intake of lactating sows and increase milk production, thereby ensuring the growth and development of piglets, thereby increasing the daily weight gain of piglets.

Claims

1. A functional pig feed composition, wherein: The pig compound feed composition comprises a fermented corn undersize feed prepared by the following steps: (1) mixing corn undersize, enzyme preparation and inorganic salt with corn, soybean meal, soybean hulls, bran, sugar residue and molasses, wherein the enzyme preparation includes papain, and the inorganic salt includes ammonium salt, potassium salt and manganese salt in a mass ratio of 2:2:1; (2) inoculating the mixture obtained in step (1) with a composite bacterial strain to obtain a material to be fermented, wherein the composite bacterial strain is composed of Lactobacillus plantarum 1.557 (CGMCC), Candida utilis 2.1180 (CGMCC) and Bacillus subtilis 1.1413 (CGMCC), wherein the bacterial count ratio of Lactobacillus plantarum 1.557 (CGMCC), Candida utilis 2.1180 (CGMCC) and Bacillus subtilis 1.1413 (CGMCC) is (40-50):(10-70):(0.5-4); (3) Fermenting the material to be fermented, and drying, cooling, and crushing the fermentation product to obtain the corn undersize fermented feed.

2. The pig compound feed composition according to claim 1, wherein The pig compound feed composition further comprises: corn, second flour, rice bran, rice bran meal, soybean oil, soybean meal, fish meal, flaxseed, sumac fatty acid calcium, brown sugar, stone powder, calcium hydrogen phosphate, sodium chloride, choline chloride, L-lysine sulfate, DL-methionine, L-threonine, potassium chloride, organic iron, phytase, Rayco 119 pig vitamin premix, A5702 pig trace element premix, ethoxyquin 60% and mildew inhibitor.

3. The pig compound feed composition according to claim 2, wherein: Based on the total weight of the pig compound feed composition, the pig compound feed composition comprises: 40wt%-55wt% of the corn, 1wt%-10wt% of the second flour, 1wt%-10wt% of the rice bran, 0.5wt%-5.0wt% of the rice bran meal, 0.1wt%-1.0wt% of the soybean oil, 10wt%-20wt% of the soybean meal, 0.5wt%-2wt% of the sumac fatty acid calcium, 1.0wt%-3.0wt% of the fish meal, 2.5wt%-4.5wt% of the flax seed, 5wt%-20wt% of the corn undersize fermented feed, 0.5wt%-2.5wt% of the brown sugar, 0.1wt%-0.7wt% of the stone powder, 0.6wt%-2wt% of the calcium hydrogen phosphate, 0.1wt%-1.0wt% of the soybean oil, 10wt%-20wt% of the soybean meal, 0.5wt%-2wt% of the sumac fatty acid calcium, 1.0wt%-3.0wt% of the fish meal, 2.5wt%-4.5wt% of the flax seed, 5wt%-20wt% of the corn undersize fermented feed, 0.5wt%-2.5wt% of the brown sugar, %-1wt% of the sodium chloride, 0.01wt%-0.5wt% of the choline chloride, 0.1wt%-0.7wt% of the L-lysine sulfate, 0.01wt%-0.1wt% of the DL-methionine, 0.02wt%-0.15wt% of the L-threonine, 0.05wt%-0.5wt% of the potassium chloride, 0.01wt%-0.15wt% of the organic iron, 0.01wt%-0.1wt% of the phytase, 0.01wt%-0.15wt% of the Rayco 119 pig vitamin premix, 0.1wt%-0.5wt% of the A5702 pig trace element premix, 0.005wt%-0.05wt% of the ethoxyquinoline 60%, and 0.01wt%-0.3wt% of the antifungal agent.

4. The pig compound feed composition according to any one of claims 1 to 3, wherein Relative to the total mass of the material to be fermented, the content of the corn undersize is 10wt%-50wt%.

5. The pig compound feed composition according to any one of claims 1 to 3, wherein The inoculation amount of the composite bacteria is 0.5%-10%.

6. The pig compound feed composition according to any one of claims 1 to 3, wherein The ammonium salt is one or more selected from the following: ammonium chloride, ammonium sulfate, ammonium carbonate; The potassium salt is one or more selected from the following: potassium sulfate, potassium chloride, potassium dihydrogen phosphate, dipotassium hydrogen phosphate, potassium phosphate; The manganese salt is one or more selected from the following: manganese acetate, manganese sulfate, and manganese chloride.

7. The pig compound feed composition according to any one of claims 1 to 3, wherein The inorganic salts include ammonium sulfate, potassium dihydrogen phosphate and manganese sulfate.

8. The pig compound feed composition according to any one of claims 1 to 3, wherein Relative to the total mass of the material to be fermented, the added amount of the inorganic salt is 0.3wt%-1.0wt%.

9. The pig compound feed composition according to any one of claims 1 to 3, wherein Relative to the total mass of the material to be fermented, the added amount of the enzyme preparation is 0.3wt%-1.0wt%.

10. The pig compound feed composition according to any one of claims 1 to 3, wherein The fermentation is carried out at 20°C to 50°C for 1 to 10 days.

11. A method for feeding lactating sows, wherein: The method comprises feeding a lactating sow with the functional pig compound feed composition according to any one of claims 1 to 10.

12. The method of claim 11, wherein: The method comprises: on the first day after farrowing, feeding each lactating sow with 1.5kg-2.5kg of the pig compound feed composition per day; on the second to fifth days after farrowing, feeding each lactating sow with 0.2kg-1kg more of the pig compound feed composition per day than the previous day; and after the sixth day after farrowing, the sows are allowed to eat freely.

13. Use of the functional pig compound feed composition according to any one of claims 1 to 10 in increasing the average daily feed intake and / or milk production of lactating sows, or increasing the daily weight gain of piglets.

14. The use according to claim 13, wherein The average daily feed intake of lactating sows is increased by more than 10wt%.

15. The use according to claim 13 or 14, wherein The daily weight gain of the piglets is increased by more than 5wt%.

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