A microfluidic detection card for multi-parameter biochemical molecular detection

Abstract

The present invention provides a microfluidic detection card for multi-parameter biochemical molecular detection, specifically comprising a detection card body (1), a microfluidic system (2) and an array electrode mounting slot (3) arranged in the detection card body (1), and an electrode card (4) loaded in the array electrode mounting slot (3), wherein a plurality of microelectrodes are integrated on the surface of the electrode card (4); the microfluidic system (2) comprises an injection port (21), an injection pipeline system, a calibration liquid system, an electrode assembly area (27) and a liquid derivation system; the electrode assembly area (27) is provided with an array electrode mounting slot (3), the array electrode mounting slot (3) is composed of a plurality of electrode mounting slots arranged in an array, a single slot can be loaded with an electrode card (4), a plurality of microelectrodes are integrated on the front of the single electrode card (4), and the same number of electrode pins are provided on the back of the electrode card (4), and the microelectrodes and the electrode pins are connected to the metal circuit one by one through the conductive sites on the surface of the electrode sheet (4).

Description

Technical Field

[0001] The invention belongs to the field of biomedical sensors and relates to a microfluidic detection card for multi-parameter biochemical molecule detection. Background Art

[0002] The latest POCT chemical sensor instruments can test multiple samples simultaneously, significantly reducing testing time and making real-time testing faster. POCT devices are often implemented using biosensors, which immobilize bioenzyme molecules on the solid-phase interface of a microanalytical device. After specifically identifying the analyte, they use electrochemical or optical methods for detection and provide immediate readings. Typical commercial biosensor-based POCT devices include blood glucose meters and blood gas analyzers, which are primarily used for blood glucose monitoring, blood gas analysis, and electrolyte analysis.

[0003] There are currently technologies for biochemical testing consumables that can detect multiple substances at once. Specifically, microelectrodes that detect specific biochemical molecules are integrated into the overall detection device and applied to multi-parameter electrochemical detection. Currently, there are technologies that combine microfluidic systems with POCT products both domestically and internationally. Microfluidics is a technology that controls the operation of microfluidics on a test card with microchannels. Existing POCT-related microfluidic systems still have defects, such as solidification and blockage of the test liquid in the microfluidic channel, easy backflow and miscibility of the test liquid, slow liquid flow rate, low detection efficiency, and waste gas generated by the reaction that affects the detection.

[0004] In view of this, the present invention aims to provide a microfluidic test card for multi-parameter biochemical molecular detection, which integrates the functions of preventing the coagulation and blockage of the test liquid, mixing the calibration liquid, sample liquid detection, waste liquid recovery and waste gas discharge on a single test card, thereby reducing the cost and processing difficulty of the test card, improving the detection speed, sensitivity and accuracy while ensuring the simultaneous detection of multiple parameters, and realizing the simultaneous detection of multiple indicators in biochemical electrolytes. Summary of the Invention

[0005] In summary, the present invention provides a microfluidic detection card for multi-parameter biochemical molecular detection.

[0006] The purpose of the present invention is to provide a microfluidic detection card for multi-parameter biochemical molecular detection, specifically comprising a detection card body (1), a microfluidic system (2) and an array electrode mounting slot (3) arranged in the detection card body (1), and an electrode card (4) loaded in the array electrode mounting slot (3), wherein a plurality of microelectrodes are integrated on the surface of the electrode card (4); the microfluidic system (2) comprises an injection port (21), an injection pipe system, a calibration liquid system, an electrode assembly area (27) and a liquid derivation system; the electrode assembly area (27) is provided with an array electrode mounting slot (3), the array electrode mounting slot (3) is composed of a plurality of electrode mounting slots arranged in an array, and a single slot can be loaded with one electrode card (4). The front of the single-chip electrode card (4) is integrated with multiple microelectrodes, and the back of the electrode card (4) is provided with the same number of electrode pins, and the microelectrodes and the electrode pins are connected to the metal circuit one by one through the conductive sites on the surface of the electrode sheet (4); each card slot of the array-type electrode mounting card slot (3) is provided with a sample test slot (31), and the electrode card (4) is loaded into the card slot with the front side facing downward, and the microelectrodes modified on the surface of the electrode card (4) are correspondingly embedded in the sample test slot (31); one end of the electrode assembly area (27) is connected to the sample injection pipeline system, and the other end is connected to the liquid derivation system; the sample injection pipeline system passes through the electrode assembly area (27), specifically passes through each card slot of the array-type electrode mounting card slot (3), and is connected to the sample test slot (31).

[0007] Furthermore, an exhaust hole (11) is provided on the detection card body (1), and the exhaust hole (11) is connected to the liquid derivation system.

[0008] Furthermore, a detection card positioning hole (12) is provided on the detection card body (1).

[0009] In the microfluidic system (2), the sampling pipeline system includes a pretreatment chamber (22), a pretreatment buffer channel (23), a micro check valve (24) and a sampling liquid channel. The sampling liquid channel is connected to the sampling port (21) and is sequentially connected to the pretreatment chamber (22), the pretreatment buffer channel (23) and the micro check valve (24). The calibration liquid system is connected to the micro check valve (24).

[0010] Furthermore, the pre-treatment chamber (22) may be filled with immunomagnetic beads, anticoagulant drugs, or left empty.

[0011] Furthermore, the pre-treatment buffer channel (23) can be a micro-circulation channel composed of a plurality of micro-saddle-shaped structures.

[0012] In the microfluidic system (2), the calibration liquid system includes a calibration liquid channel and a calibration liquid bag (26), a diversion valve (25) is provided between the calibration liquid channel and the calibration liquid bag (26); the calibration liquid channel is connected to the sampling pipeline system.

[0013] In the microfluidic system (2), the liquid outlet system includes a simple check valve (28), a waste liquid tank (29), and a sample liquid outlet channel. The sample liquid outlet channel is connected to the sample inlet pipe system, and the sample liquid outlet channel is connected to the simple check valve (28) and the waste liquid tank (29) in sequence.

[0014] Furthermore, the simple check valve (28) structure includes a Tesla valve structure.

[0015] In the array-type electrode mounting slot (3), the sample testing slot (31) is a groove of fixed volume.

[0016] Furthermore, a positioning column (32) is provided in each card slot of the array-type electrode mounting card slot (3), and a positioning hole (41) is provided on the surface of the electrode card (4). The positioning column (32) corresponds to the position of the positioning hole (41) and has the same size as the positioning hole (41), and the electrode card (4) can be fixed inside the array-type electrode mounting card slot (8) through the positioning hole (41).

[0017] The microelectrodes integrated on the surface of the electrode card (4) include a reference electrode, an auxiliary electrode and a working electrode, wherein the working electrode includes a calibration electrode.

[0018] Furthermore, the surface-modified microelectrodes of the electrode card (4) include a reference electrode (51), an auxiliary electrode (52), a working electrode A (53), a working electrode B (54) and a calibration electrode (55).

[0019] As attached Figure 1 and attached Figure 2 , Attachment Figure 3 , which are respectively a schematic diagram of the structure, front and back of the microfluidic detection card provided by the present invention.

[0020] The injection port (21) can be used to inject blood / test samples into the microfluidic pipeline through a 1~3 ml syringe; the pretreatment chamber (22) is used for pretreatment of the injection solution, such as separation, filtration, mixing, etc. Magnetic beads can be placed in the pretreatment chamber (22) for molecular extraction of RNA and ctDNA, that is, mixed with the sample for DNA detection. In addition, the pretreatment chamber (22) can also be placed with other substances, structures or be left empty; the pretreatment buffer channel (23) has the function of mixing evenly and reducing the flow rate, which can improve the pretreatment efficiency of the injection solution and prevent the pretreatment chamber from The reaction in (22) is insufficient; the micro check valve (24) has a micro inverted cone-shaped structure, which is vertical when the test card is placed horizontally, with the opening larger at the top and smaller at the bottom, and prevents the sample from flowing back through the flow resistance; the diverter valve (25) can guide the calibration liquid to the correct flow direction, and at the same time has a structure with a certain height difference to prevent the liquid from flowing back into the calibration liquid package (26), specifically, the calibration liquid package (26) is higher in direction and lowers along the outflow direction of the calibration liquid; the calibration liquid package (26) is used to load the calibration liquid of standard concentration, and the calibration liquid can flow out through mechanical devices or manual squeezing.

[0021] The electrode assembly area (27) is a region dug out on the detection card body (1) for sample detection, which is a card slot (3) for mounting the array electrode. Specifically, it can be divided into 6 card slots of the same specifications. A groove of a fixed volume is provided in each card slot, which serves as a sample test slot (31). At the same time, positioning posts (32) are provided at the two corners. The positions of the sample test slot (31) and the positioning posts (32) correspond to the microelectrode detection site and the positioning hole (41) on the electrode card (4), respectively. When the electrode card (4) is installed inside the card slot, its integrated microelectrode is fixed in the sample test slot (31).

[0022] As attached Figure 4 and attached Figure 5 The following are schematic diagrams of the front and back structures of the electrode card.

[0023] The front of the electrode card (4) is integrated with five microelectrodes, including one reference electrode, one auxiliary electrode, and three working electrodes, one of which can be used as a calibration electrode. The reference electrode (51) is surface-modified with silver-silver chloride, the auxiliary electrode (52) is surface-modified with a platinum layer, and the working electrode can be surface-modified with a gold layer. Different sensitive substances can also be modified according to the detection index. Each electrode card can detect at least two substance indicators simultaneously.

[0024] The calibration electrode (55) can achieve the purpose of self-calibration, making the working electrode detection result more accurate.

[0025] The back of the electrode card (4) is provided with metal pins having the same number as the microelectrodes, and the microelectrodes and the metal pins are connected to each other through the internal metal circuits of the electrode card (4) or the metal circuits of the conductive holes.

[0026] The electrode card (4) is installed with its front side facing downward inside the array electrode installation slot (3), and the microelectrodes are correspondingly embedded in the sample test slot (31).

[0027] The number of slots of the array-type electrode mounting slots (3) can be changed according to the number of detection indicators. At the same time, the size of the slots and the size of the electrode cards (4) can be changed according to demand. The number of positioning columns (32) can also be changed according to the number and size of the electrode cards (4).

[0028] The sample inlet liquid channel of the test card body (1) passes through the six sample test slots (31) of the array electrode mounting slot (3) in sequence and is connected to the sample outlet liquid channel at the outlet. The simple check valve (28) adopts a special structure to prevent the liquid in the waste liquid tank from flowing back; the waste liquid tank (29) can be loaded with tested liquid, including calibration liquid and test samples. The exhaust hole (11) is used to discharge the gas in the flow channel, which facilitates the flow of liquid in the microchannel and has a simple anti-overflow structure.

[0029] All of the above structures, except for the electrode card (4) that needs to be installed separately, are included in the detection card body (1) and can be completed in one go through the injection molding method without the need to install additional parts. This greatly simplifies the processing steps of the microfluidic system and realizes the integration of microfluidics and sensor detection cards. The electrode card is fixed inside the detection card slot, and multiple biochemical molecules are detected simultaneously through the microelectrode integration solution, thereby realizing multi-parameter electrochemical detection of the microfluidic sensor detection card.

[0030] As attached Figure 6 , which is a schematic diagram of liquid injection of the microfluidic detection card provided by the present invention.

[0031] When adding samples, the test card is placed horizontally with the front side facing upwards, and the liquid to be tested is injected from the sample inlet (21), flows through the pretreatment chamber (22), flows into the pretreatment buffer channel (23), and then flows through the micro-check valve (24). At the same time, a mechanical device or manual force is used to squeeze the calibration liquid package (26), squeeze out the calibration liquid in the calibration liquid package (26), and then flows into the calibration liquid channel after passing through the guide valve (25), and then passes through the micro-check valve (24) to mix with the liquid to be tested in the sample liquid channel; the mixed liquid passes through the sample test slot (31) and the microelectrode modified on the front side of the contact electrode card (4) through the sample liquid channel, and then passes through the sample liquid outlet channel, flows through the simple check valve (28), and enters the waste liquid tank (29). The gas generated on the way is discharged through the exhaust hole (11) connected to the waste liquid tank (29), and the detection electrical signal is transmitted through the electrode pins on the back side of the electrode card (4).

[0032] The beneficial effects of the present invention are:

[0033] Through microfluidic structure design, the present invention integrates pre-treatment functions such as preventing coagulation and blockage of the test liquid, calibration liquid mixing, sample liquid detection, waste liquid recovery and waste gas emission on a single test card, reducing the cost and processing difficulty of the test card, improving the detection speed, sensitivity and accuracy while ensuring the simultaneous detection of multiple parameters, and realizing the simultaneous detection of multiple indicators in biochemical electrolytes.

[0034] The microfluidic test card of the present invention is designed with a sample test slot and a fixed measurement volume, making the measurement results more accurate. At the same time, the positioning column design makes it possible to separate the microfluidic test card and the electrode card, simplifying the structure of the microfluidic test card and greatly reducing the difficulty and efficiency of its production process. The electrode card can be installed according to the detection target, and is suitable for the analysis of multiple indicators in blood gas testing.

[0035] The array electrode sheet is loaded into the card slot of the microfluidic detection card, and multiple microelectrodes are integrated on the electrode sheet. Combined with the microfluidic sensor detection card, it can realize the simultaneous detection of multiple biochemical molecules and can be applied to multi-parameter electrochemical detection. BRIEF DESCRIPTION OF THE DRAWINGS

[0036] The invention is further described with reference to the accompanying drawings, but the embodiments in the accompanying drawings do not constitute any limitation to the invention. A person skilled in the art can obtain other drawings based on the following drawings without inventive effort.

[0037] Figure 1 Schematic diagram of the structure of the microfluidic detection card provided by the present invention;

[0038] Figure 2 1 is a front schematic diagram of the microfluidic detection card provided by the present invention;

[0039] Figure 3 1 is a schematic diagram of the back side of the microfluidic detection card provided by the present invention;

[0040] Figure 4 This is a schematic diagram of the front structure of the electrode card;

[0041] Figure 5 This is a schematic diagram of the structure on the back of the electrode card;

[0042] Figure 6 It is a schematic diagram of liquid injection of the microfluidic detection card provided by the present invention.

[0043] Legend:

[0044] Detection card body; 11. Exhaust hole; 12. Detection card positioning hole;

[0045] Microfluidic system; 21. Inlet; 22. Pretreatment chamber; 23. Pretreatment buffer channel; 24. Micro check valve; 25. Diversion valve; 26. Calibration liquid package; 27. Electrode assembly area; 28. Simple check valve; 29. ​​Waste liquid tank;

[0046] Array electrode mounting slot; 31. Sample test slot; 32. Positioning column;

[0047] Electrode card; 41, positioning hole;

[0048] 51. Reference electrode; 52. Auxiliary electrode; 53. Working electrode A; 54. Working electrode B; 55. Calibration electrode. DETAILED DESCRIPTION

[0049] In order to make the objectives, technical solutions and advantages of the present invention more clearly understood, the present invention is further described in detail with reference to the following specific embodiments and the accompanying drawings.

[0050] Example 1

[0051] As attached Figures 1 to 3 As shown, the microfluidic detection card provided by the present invention specifically includes a detection card body 1, and the detection card body has a width of 70 mm, a height of 70 mm, and a thickness of 3.0 mm.

[0052] In this embodiment, the sampling port 21 on the detection card body 1 is provided with a sampling pipe therein, which has a width of 1.0 mm and a height of 1.0 mm.

[0053] In this embodiment, the sampling pipe system and the calibration liquid package 26 are located inside the test card body 1, and the calibration liquid package 26 has a width of 13 mm and a height of 31 mm.

[0054] In this embodiment, the sampling pipeline system includes a pretreatment chamber 22, a pretreatment buffer channel 23 and a micro check valve 24 which are connected to the sampling port in sequence. The electrode assembly area 27 is connected to the sampling pipeline system through an injection liquid channel buried inside the detection card body 1. The injection liquid channel is connected to the micro check valve 24, and a calibration liquid channel is extended through the micro check valve 24 to be connected to the calibration liquid bag 26. A diverter valve 25 is provided between the calibration liquid channel and the calibration liquid bag 26.

[0055] The pre-treatment chamber 22 is filled with immune microspheres.

[0056] The pre-treatment buffer channel 23 is a micro-circulation channel composed of a plurality of micro-saddle-shaped structures.

[0057] The width of the injection liquid channel and the calibration liquid channel is 1.0 mm, and the height is 1.0 mm.

[0058] In this embodiment, the electrode assembly area 27 and the liquid derivation system on the back of the detection card body 1 are detected.

[0059] In this embodiment, one end of the electrode assembly area 27 is connected to the sample liquid channel, and the other end is connected to the liquid outlet system through the sample liquid outlet channel buried in the detection card body 1; the liquid outlet system is connected to the exhaust hole 11.

[0060] In this embodiment, the electrode assembly area 27 is a region dug out on the detection card body 1 for sample detection, and is used for array electrode mounting slots 3, which can be specifically divided into two rows of 6 slots of uniform specifications. Each slot 8 is provided with a groove of a fixed volume, which serves as a sample test slot 31. At the same time, positioning posts 32 are provided at the corners. The positions of the sample test slot 31 and the positioning posts 32 correspond to the microelectrode detection sites and positioning holes 41 on the electrode card 4, respectively.

[0061] like Figure 4~Figure 5 As shown, the front of the electrode card 4 is integrated with five microelectrodes: one reference electrode, one auxiliary electrode, and three working electrodes, one of which can be used as a calibration electrode. These are reference electrode 51, auxiliary electrode 52, working electrode A 53, working electrode B 54, and calibration electrode 55. Reference electrode 51 is modified with silver-silver chloride, auxiliary electrode 52 is modified with platinum, and the working electrodes can be modified with gold. Different sensitive substances can also be modified based on the test indicator. Each electrode card 4 can simultaneously detect two substance indicators.

[0062] The back of the electrode card 4 is provided with metal pins having the same number as the microelectrodes, and the microelectrodes and the metal pins are correspondingly connected through the internal metal circuits of the electrode card 4 or the metal circuits of the conductive holes.

[0063] The surface of the detection card body 1 is loaded with 6 electrode cards 4. The front of the electrode card 4 is installed downward inside the array electrode installation card slot 3. The microelectrodes are correspondingly embedded in the sample test slot 31. The sample inlet liquid channel passes through all the sample test slots 31 in sequence and is connected to the sample outlet liquid channel, so that the liquid to be tested contacts the microelectrode when passing through the sample test slot 31; the back of the electrode card 4 faces upward, exposing the electrode pins. The electrical signal changes of the microelectrode can be collected by connecting the electrode pins, and the concentration of the analyte detected by the microelectrode is detected by electrochemical methods.

[0064] Each electrode sheet can detect 2 different targets, and the microfluidic detection card as a whole can detect 12 different material indicators at the same time, realizing multi-parameter instant electrochemical detection.

[0065] The sample test slot 31 is a fixed-volume groove with a width of 8.1 mm and a height of 1.5 mm. The positioning column 32 is cylindrical with a radius of 0.2 mm. The width of a single slot of the array electrode mounting slot 3 is 13.4 mm and the height is 7.0 mm.

[0066] In this embodiment, the liquid outlet system includes a simple check valve 28 , a waste liquid tank 29 and an exhaust hole 11 .

[0067] The structure of the simple check valve 28 is a Tesla valve structure.

[0068] In this embodiment, the test card body 1 is further provided with a test card positioning hole 12 for fixing the test card to a test instrument or other device, and the hole diameter is 1.0 mm.

[0069] Example 2

[0070] This embodiment provides a microfluidic detection card for multi-parameter biochemical molecular detection, which specifically includes a detection card body 1. The detection card body 1 has a width of 70 mm, a height of 70 mm, and a thickness of 3.0 mm.

[0071] In this embodiment, the sampling port 21 on the detection card body 1 is provided with a sampling pipe therein, which has a width of 1.5 mm and a height of 1.5 mm.

[0072] In this embodiment, the sampling pipe system and the calibration liquid package 26 are located on the front of the test card body 1, and the calibration liquid package 26 has a width of 12 mm and a height of 30 mm.

[0073] In this embodiment, the sampling pipeline system includes a pretreatment chamber 22, a pretreatment buffer channel 23 and a micro check valve 24 which are connected to the sampling port in sequence. The electrode assembly area 27 is connected to the sampling pipeline system through an injection liquid channel buried inside the detection card body 1. The injection liquid channel is connected to the micro check valve 24, and a calibration liquid channel is extended through the micro check valve 24 to be connected to the calibration liquid bag 26. A diverter valve 25 is provided between the calibration liquid channel and the calibration liquid bag 26.

[0074] Anticoagulant drugs are placed in the pre-treatment chamber 22 .

[0075] The pre-treatment buffer channel 23 is a micro-circulation channel composed of a plurality of micro-saddle-shaped structures.

[0076] The width of the injection liquid channel and the calibration liquid channel is 1.0 mm, and the height is 1.0 mm.

[0077] In this embodiment, the electrode assembly area 27 and the liquid derivation system on the back of the detection card body 1 are detected.

[0078] In this embodiment, one end of the electrode assembly area 27 is connected to the sample liquid channel, and the other end is connected to the liquid outlet system through the sample liquid outlet channel buried in the detection card body 1; the liquid outlet system is connected to the exhaust hole 11.

[0079] In this embodiment, the electrode assembly area 27 is a region dug out on the detection card body for sample detection, which is used for array electrode installation slots 3. Specifically, it can be divided into two rows, with a total of 4 slots of the same specifications. Each slot is provided with a groove of a fixed volume, which serves as a sample test slot 31. At the same time, positioning posts 32 are provided at the corners. The positions of the sample test slot 31 and the positioning posts 32 correspond to the microelectrode detection sites and positioning holes 41 on the electrode card 4, respectively.

[0080] The front of the electrode card 4 is integrated with five microelectrodes: one reference electrode, one auxiliary electrode, and three working electrodes, one of which can be used as a calibration electrode. These are reference electrode 51, auxiliary electrode 52, working electrode A 53, working electrode B 54, and calibration electrode 55. The reference electrode is modified with silver-silver chloride, the auxiliary electrode with platinum, and the working electrode with gold. These can also be modified with different sensitive substances depending on the test indicator. Each electrode card 4 can simultaneously detect two substance indicators.

[0081] The back of the electrode card 4 is provided with metal pins having the same number as the microelectrodes, and the microelectrodes and the metal pins are correspondingly connected through the internal metal circuits of the electrode card 4 or the metal circuits of the conductive holes.

[0082] The surface of the detection card body 1 is loaded with four electrode cards 4. The front of the electrode card 4 is installed downward inside the array electrode installation card slot 3. The microelectrodes are correspondingly embedded in the sample test slot 31. The sample inlet liquid channel passes through all the sample test slots 31 in sequence and is connected to the sample outlet liquid channel, so that the liquid to be tested contacts the microelectrode when passing through the sample test slot 31; the back of the electrode card 4 faces upward, exposing the electrode pins. The electrical signal changes of the microelectrode can be collected by connecting the electrode pins, and the concentration of the analyte detected by the microelectrode is detected by electrochemical methods.

[0083] Each electrode sheet can detect 2 different targets, and the microfluidic detection card as a whole can simultaneously detect 8 different material indicators, realizing multi-parameter real-time electrochemical detection.

[0084] The sample test slot 31 is a fixed-volume groove with a width of 8.0 mm and a height of 1.5 mm. The positioning column is cylindrical with a radius of 0.2 mm. The width of a single slot of the array electrode mounting slot 3 is 14.5 mm and the height is 7.5 mm.

[0085] In this embodiment, the liquid outlet system includes a simple check valve 28 , a waste liquid tank 29 and an exhaust hole 11 .

[0086] The structure of the simple check valve 28 is a Tesla valve structure.

[0087] In this embodiment, the detection card body 1 is further provided with a detection card positioning hole 12 with a hole diameter of 1.0 mm.

[0088] Example 3

[0089] This embodiment provides a microfluidic detection card for multi-parameter biochemical molecular detection, which specifically includes a detection card body 1. The detection card body 1 has a width of 70 mm, a height of 70 mm, and a thickness of 3.0 mm.

[0090] In this embodiment, the sampling port 21 on the detection card body 1 is provided with a sampling pipe therein, which has a width of 1.5 mm and a height of 1.5 mm.

[0091] In this embodiment, the sampling pipe system and the calibration liquid package 26 are located on the front of the test card body 1, and the calibration liquid package 26 has a width of 12 mm and a height of 30 mm.

[0092] In this embodiment, the sampling pipeline system includes a pretreatment chamber 22, a pretreatment buffer channel 23 and a micro check valve 24 which are connected to the sampling port in sequence. The electrode assembly area 27 is connected to the sampling pipeline system through an injection liquid channel buried inside the detection card body 1. The injection liquid channel is connected to the micro check valve 24, and a calibration liquid channel is extended through the micro check valve 24 to be connected to the calibration liquid bag 26. A diverter valve 25 is provided between the calibration liquid channel and the calibration liquid bag 26.

[0093] The pre-processing chamber 22 is empty.

[0094] The pre-treatment buffer channel 23 is a micro-circulation channel composed of a plurality of micro-saddle-shaped structures.

[0095] The width of the injection liquid channel and the calibration liquid channel is 1.5 mm, and the height is 1.5 mm.

[0096] In this embodiment, the electrode assembly area 27 and the liquid derivation system on the back of the detection card body 1 are detected.

[0097] In this embodiment, one end of the electrode assembly area 27 is connected to the sample liquid channel, and the other end is connected to the liquid outlet system through the sample liquid outlet channel buried in the detection card body 1; the liquid outlet system is connected to the exhaust hole 11.

[0098] In this embodiment, the electrode assembly area 27 is a region dug out on the detection card body for sample detection, which is an array electrode mounting slot 3, specifically divided into a row of 3 slots of the same specifications. Each slot is provided with a groove of a fixed volume, which serves as a sample test slot 31. At the same time, positioning posts 32 are provided at the corners. The positions of the sample test slot 31 and the positioning posts 32 correspond to the microelectrode detection sites and positioning holes 41 on the electrode card 4, respectively, so that the electrode card 4 can be installed and fixed in the sample test slot 31.

[0099] The front of the electrode card 4 is integrated with five microelectrodes: one reference electrode, one auxiliary electrode, and three working electrodes, one of which can be used as a calibration electrode. These are reference electrode 51, auxiliary electrode 52, working electrode A 53, working electrode B 54, and calibration electrode 55. The reference electrode is modified with silver-silver chloride, the auxiliary electrode with platinum, and the working electrode with gold. These can also be modified with different sensitive substances depending on the test indicator. Each electrode card 4 can simultaneously detect two substance indicators.

[0100] The back of the electrode card 4 is provided with metal pins having the same number as the microelectrodes, and the microelectrodes and the metal pins are correspondingly connected through the internal metal circuits of the electrode card 4 or the metal circuits of the conductive holes.

[0101] The surface of the detection card body 1 is loaded with three electrode cards 4. The front of the electrode card 4 is installed downward inside the array electrode installation card slot 3. The microelectrodes are correspondingly embedded in the sample test slot 31. The sample inlet liquid channel passes through all the sample test slots 31 in sequence and is connected to the sample outlet liquid channel, so that the liquid to be tested contacts the microelectrode when passing through the sample test slot 31; the back of the electrode card 4 faces upward, exposing the electrode pins. The electrical signal changes of the microelectrode can be collected by connecting the electrode pins, and the concentration of the analyte detected by the microelectrode is detected by electrochemical methods.

[0102] Each electrode sheet can detect 2 different targets, and the microfluidic detection card as a whole can detect 6 different material indicators at the same time, realizing multi-parameter instant electrochemical detection.

[0103] The sample test slot 31 is a fixed-volume groove with a width of 8.0 mm and a height of 1.5 mm. The positioning column is cylindrical with a radius of 0.2 mm. The width of a single electrode mounting slot is 13.0 mm and the height is 6.5 mm.

[0104] In this embodiment, the liquid outlet system includes a simple check valve 28 , a waste liquid tank 29 and an exhaust hole 11 .

[0105] The structure of the simple check valve 28 is a Tesla valve structure.

[0106] In this embodiment, a detection card positioning hole 12 is further provided on the detection card body, and the hole diameter is 1.0 mm.

[0107] Example 4

[0108] This embodiment provides a microfluidic detection card for multi-parameter biochemical molecular detection, which specifically includes a detection card body 1. The detection card body 1 has a width of 70 mm, a height of 70 mm, and a thickness of 3.0 mm.

[0109] In this embodiment, the sampling port 21 on the detection card body 1 is provided with a sampling pipe therein, which has a width of 1.5 mm and a height of 1.5 mm.

[0110] In this embodiment, the sampling pipe system and the calibration liquid package 26 are located on the front of the test card body 1, and the calibration liquid package 26 has a width of 12 mm and a height of 30 mm.

[0111] In this embodiment, the sampling pipeline system includes a pretreatment chamber 22, a pretreatment buffer channel 23 and a micro check valve 24 which are connected to the sampling port in sequence. The electrode assembly area 27 is connected to the sampling pipeline system through an injection liquid channel buried inside the detection card body 1. The injection liquid channel is connected to the micro check valve 24, and a calibration liquid channel is extended through the micro check valve 24 to be connected to the calibration liquid bag 26. A diverter valve 25 is provided between the calibration liquid channel and the calibration liquid bag 26.

[0112] The pre-processing chamber 22 is empty.

[0113] The pre-treatment buffer channel 23 is a micro-circulation channel composed of a plurality of micro-saddle-shaped structures.

[0114] The width of the injection liquid channel and the calibration liquid channel is 1.5 mm, and the height is 1.5 mm.

[0115] In this embodiment, the electrode assembly area 27 and the liquid derivation system on the back of the detection card body 1 are detected.

[0116] In this embodiment, one end of the electrode assembly area 27 is connected to the sample liquid channel, and the other end is connected to the liquid outlet system through the sample liquid outlet channel buried in the detection card body 1; the liquid outlet system is connected to the exhaust hole 11.

[0117] In this embodiment, the electrode assembly area 27 is a region dug out on the detection card body 1 for sample detection, in which an electrode mounting slot is provided, and a groove of a fixed volume is provided in the electrode mounting slot, which serves as a sample test slot 31. At the same time, positioning posts 32 are provided at the corners. The positions of the sample test slot 31 and the positioning posts 32 correspond to the microelectrode detection sites and positioning holes 41 on the electrode card 4, respectively.

[0118] The front of the electrode card 4 is integrated with five microelectrodes: one reference electrode, one auxiliary electrode, and three working electrodes, one of which can be used as a calibration electrode. These are reference electrode 51, auxiliary electrode 52, working electrode A 53, working electrode B 54, and calibration electrode 55. The reference electrode is modified with silver-silver chloride, the auxiliary electrode with platinum, and the working electrode with gold. These can also be modified with different sensitive substances depending on the test indicator. Each electrode card 4 can simultaneously detect two substance indicators.

[0119] The back of the electrode card 4 is provided with metal pins having the same number as the microelectrodes, and the microelectrodes and the metal pins are correspondingly connected through the internal metal circuits of the electrode card 4 or the metal circuits of the conductive holes.

[0120] An electrode card 4 is mounted on the surface of the detection card body 1. The front of the electrode card 4 is installed downward inside the electrode installation slot. The microelectrode is correspondingly embedded in the sample test slot 31. The sample inlet liquid channel passes through all the sample test slots 31 in sequence and is connected to the sample outlet liquid channel, so that the liquid to be tested contacts the microelectrode when passing through the sample test slot 31; the back of the electrode card 4 faces upward, exposing the electrode pins. The electrical signal changes of the microelectrode can be collected by connecting the electrode pins, and the concentration of the analyte detected by the microelectrode is detected by electrochemical methods.

[0121] Each electrode sheet 4 can detect 2 different targets, and the microfluidic detection card as a whole can detect 2 different material indicators at the same time, realizing multi-parameter instant electrochemical detection.

[0122] The sample test slot 31 is a fixed-volume groove with a width of 8.0 mm and a height of 1.5 mm. The positioning column is cylindrical with a radius of 0.2 mm. The electrode mounting slot is 13.0 mm wide and 6.5 mm high.

[0123] In this embodiment, the liquid outlet system includes a simple check valve 28 , a waste liquid tank 29 and an exhaust hole 11 .

[0124] The structure of the simple check valve 28 is a Tesla valve structure.

[0125] In this embodiment, the detection card body 1 is further provided with a detection card positioning hole 12 with a hole diameter of 1.0 mm.

[0126] Example 5

[0127] According to the multi-parameter biochemical molecular detection microfluidic detection card provided in Example 1, Figure 6 , is a schematic diagram of liquid injection of the microfluidic detection card provided by the present invention.

[0128] When adding samples, the microfluidic detection card is placed horizontally with the front side facing up. The liquid to be tested is injected from the sampling port 21, flows through the pretreatment chamber 22, flows into the pretreatment buffer channel 23, and then flows through the micro check valve 24. At the same time, a mechanical device is used to squeeze the calibration liquid package 26, squeeze the calibration liquid in the calibration liquid package 26 out, pass through the guide valve 25, and flow into the calibration liquid channel, and then mix with the liquid to be tested through the micro check valve 24; the mixed liquid passes through the sample test slot 31 and the microelectrode modified on the front side of the contact electrode card 4 through the sampling liquid channel, and then passes through the sample liquid outlet channel, flows through the simple check valve 28, and enters the waste liquid tank 29. The gas generated on the way is discharged through the exhaust hole 11 connected to the waste liquid tank 29, and the detection electrical signal is transmitted through the electrode pins of the electrode card 4.

[0129] The electrode mounting slot is an array-like design, housing six identically sized electrode cards. Each card features five microelectrodes on its front, corresponding to the sample test slots 31. Inside the array-like electrode mounting slot 3, a continuous groove-shaped sample test slot 31 is located, connecting to the sample inlet channel. The test liquid flows sequentially through all sample test slots 31 through the sample inlet channel, contacting the corresponding microelectrodes, enabling simultaneous detection of up to 12 different indicators.

[0130] It will be apparent to those skilled in the art that the present invention is not limited to the details of the exemplary embodiments described above and that the invention can be embodied in other specific forms without departing from the spirit or essential characteristics of the invention. Therefore, the embodiments should be considered in all respects as illustrative and non-restrictive, and the scope of the invention is defined by the appended claims rather than the foregoing description, and it is intended that all variations that come within the meaning and range of equivalents of the claims be embraced therein.

[0131] In addition, it should be understood that although this specification is described in terms of implementation methods, not every implementation method contains only one independent technical solution. This description is for clarity only. Those skilled in the art should consider this specification as a whole. The technical solutions in each embodiment can also be appropriately combined to form other implementation methods that can be understood by those skilled in the art. Technical details not described in detail in this invention can be implemented by any existing technology in the art. In particular, all technical features not described in detail in this invention can be implemented by any existing technology.

Claims

1. A microfluidic detection card for multi-parameter biochemical molecular detection, characterized in that: The invention comprises a detection card body (1), a microfluidic system (2) and an array electrode mounting slot (3) arranged in the detection card body (1), and an electrode card (4) loaded in the array electrode mounting slot (3), wherein the surface of the electrode card (4) is integrated with a plurality of microelectrodes; the microfluidic system (2) comprises an injection port (21), an injection pipe system, a calibration liquid system, an electrode assembly area (27) and a liquid derivation system; the electrode assembly area (27) is provided with an array electrode mounting slot (3), wherein the array electrode mounting slot (3) is composed of a plurality of electrode mounting slots arranged in an array, wherein a single slot is loaded with an electrode card (4), and a single electrode card (4) is integrated with a plurality of microelectrodes on its front surface. The back of the card (4) is provided with the same number of electrode pins, and the microelectrodes and the electrode pins are connected to the metal circuits one by one through the conductive sites on the surface of the electrode card (4); each card slot of the array electrode mounting card slot (3) is provided with a sample test slot (31), and the electrode card (4) is loaded into the card slot with the front side facing downward, and the microelectrodes modified on the surface of the electrode card (4) are correspondingly embedded in the sample test slot (31); one end of the electrode assembly area (27) is connected to the sample injection pipeline system, and the other end is connected to the liquid derivation system; the sample injection pipeline system passes through the electrode assembly area (27), specifically passes through each card slot of the array electrode mounting card slot (3), and is connected to the sample test slot (31); In the microfluidic system (2), the sampling pipeline system includes a pretreatment chamber (22), a pretreatment buffer channel (23), a micro check valve (24) and a sampling liquid channel, the sampling liquid channel is connected to the sampling port (21), and is sequentially connected to the pretreatment chamber (22), the pretreatment buffer channel (23) and the micro check valve (24), and the calibration liquid system is connected to the micro check valve (24); In the microfluidic system (2), the calibration liquid system includes a calibration liquid channel and a calibration liquid bag (26), the calibration liquid bag (26) is filled with calibration liquid, and a diversion valve (25) is provided between the calibration liquid channel and the calibration liquid bag (26); the calibration liquid channel is connected to the sampling pipeline system; In the microfluidic system (2), the liquid outlet system includes a simple check valve (28), a waste liquid tank (29), and a sample liquid outlet channel. The sample liquid outlet channel is connected to the sample inlet pipe system, and the sample liquid outlet channel is connected to the simple check valve (28) and the waste liquid tank (29) in sequence.

2. A microfluidic detection card for multi-parameter biochemical molecular detection according to claim 1, characterized in that: The detection card body (1) is provided with an exhaust hole (11), and the exhaust hole (11) is connected to the liquid derivation system.

3. A microfluidic detection card for multi-parameter biochemical molecular detection according to claim 1, characterized in that: The detection card body (1) is also provided with a detection card positioning hole (12).

4. A microfluidic detection card for multi-parameter biochemical molecular detection according to claim 1, characterized in that: The pre-treatment chamber (22) is filled with immunomagnetic beads, anticoagulant drugs or is empty.

5. A microfluidic detection card for multi-parameter biochemical molecular detection according to claim 1, characterized in that: The pre-treatment buffer channel (23) is a micro-circulation channel composed of a plurality of micro-saddle-shaped structures.

6. A microfluidic detection card for multi-parameter biochemical molecular detection according to claim 1, characterized in that: The simple check valve (28) structure includes a Tesla valve structure.

7. A microfluidic detection card for multi-parameter biochemical molecular detection according to claim 1, characterized in that: In the array-type electrode installation slot (3), the sample testing slot (31) is a groove of fixed volume.

8. The microfluidic detection card for multi-parameter biochemical molecular detection according to claim 1, characterized in that: A positioning post (32) is provided in each slot of the array-type electrode mounting slot (3), and a positioning hole (41) is provided on the surface of the electrode card (4). The positioning post (32) corresponds to the position of the positioning hole (41) and has the same size as the positioning hole (41), and the electrode card (4) is fixed inside the array-type electrode mounting slot (3) through the positioning hole (41).

9. A microfluidic detection card for multi-parameter biochemical molecular detection according to claim 1, characterized in that: The microelectrodes integrated on the surface of the electrode card (4) include a reference electrode, an auxiliary electrode and a working electrode, wherein the working electrode includes a calibration electrode.

10. A microfluidic detection card for multi-parameter biochemical molecular detection according to claim 9, characterized in that: The surface-modified microelectrodes of the electrode card (4) include a reference electrode (51), an auxiliary electrode (52), a working electrode A (53), a working electrode B (54) and a calibration electrode (55).

11. A method for using the microfluidic detection card for multi-parameter biochemical molecular detection according to any one of claims 1 to 10, characterized in that: The method comprises the following steps: when adding a sample, the microfluidic detection card is placed horizontally with the front side facing upward, the liquid to be tested is injected from the sampling port (21), flows through the pretreatment chamber (22), flows into the pretreatment buffer channel (23), and then flows through the micro check valve (24); at the same time, a calibration liquid package (26) is squeezed out by a mechanical device or manual force, and the calibration liquid in the calibration liquid package (26) is squeezed out, flows into the calibration liquid channel after passing through the guide valve (25), and then is mixed with the liquid to be tested in the sampling liquid channel through the micro check valve (24); The mixed liquid passes through the sample test slot (31) and the microelectrode modified on the front of the contact electrode card (4) through the sample liquid inlet channel, and then passes through the simple check valve (28) and enters the waste liquid tank (29). The gas generated on the way is discharged through the exhaust hole (11) connected to the waste liquid tank (29), and the detection electrical signal is transmitted through the electrode pin on the back of the electrode card (4).

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