A bean curd attractant

Abstract

The invention discloses a phagocytic worm attractant, which is characterized in that the attractant comprises ethyl benzoate; glucose and potassium dihydrogen phosphate are further added to the attractant; and the preparation method of the attractant comprises the following steps: step 1: dissolving ethyl benzoate with ethanol; step 2: dissolving the ethyl benzoate ethanol solution, glucose and potassium dihydrogen phosphate in water; and the phagocytic worm can eat mulberry leaves, thereby expanding the host plant range of the phagocytic worm.

Description

Technical Field

[0001] The invention relates to the technical field of insect breeding, and in particular to a bean curd attractant. Background Art

[0002] Bean moth (Horphinidae) is a common pest in northern Jiangsu Province. Clanis Bilineata Walker ) is a common name for the larvae of the bean moth. The bean moth is an insect of the genus Sphingidae in the order Lepidoptera, and undergoes complete metamorphosis.

[0003] Although schizonepeta is a crop pest, it is nutritious and delicious, so people in many parts of my country have the habit of eating it. Guanyun County, Jiangsu Province, in particular, has developed into the country's largest schizonepeta distribution center, with an annual trading volume of tens of thousands of tons and an output value of hundreds of millions of yuan. It purchases schizonepeta from Hunan, Hubei, Anhui, Henan, Hebei, and even Northeast China, and sells it to surrounding counties and cities as well as multiple regions in Shandong Province.

[0004] The market for bean curd is huge. In August, when the bean hawk moth naturally occurs, the price of bean curd is 50-60 yuan per kilogram. In May, the price of bean curd that is put on the market in advance using greenhouse facilities or cultivated in Hainan is as high as 1,000 yuan per kilogram.

[0005] The soybean feed industry has broad development prospects, but since it has a certain impact on soybean production, it is necessary to develop new soybean feed.

[0006] The bean scorpion is a strict oligophagous insect. Current studies have found that it only feeds on the leaves of a few legumes such as soybeans, kudzu vines, locusts, and lentils. However, the above-mentioned plants are either related to national food security or are planted on a small scale, and insufficient feed supply has become a bottleneck for the large-scale increase in the yield of the bean scorpion. If its diet can be expanded to the leaves of plants other than legumes, it can effectively promote the increase in the yield of the bean scorpion, and it can also achieve indoor breeding of the bean scorpion. The inventor found that the yield of mulberry leaves is relatively high, and it can also revitalize many gradually abandoned mulberry gardens and promote the increase of mulberry farmers' income. However, the objective fact is that the bean scorpion will not eat mulberry leaves even if it starves to death, so first of all, a kind of attractant must be developed to enable the bean scorpion to open its mouth and eat mulberry leaves. Summary of the invention

[0007] The purpose of the present invention is to develop a feeding attractant for radix sphaerocephala, which is combined with high-yield mulberry leaves to realize agricultural breeding, so as to change the problem that radix sphaerocephala does not eat mulberry leaves, enable radix sphaerocephala to eat mulberry leaves, and increase the income of both mulberry leaf breeders and radix sphaerocephala breeders.

[0008] In order to achieve the above object, the technical solution adopted by the present invention is as follows:

[0009] A bean curd attractant, characterized in that the ingredient of the attractant is ethyl benzoate.

[0010] Furthermore, glucose and potassium dihydrogen phosphate are added to the ingredients of the attractant.

[0011] Further, the preparation method of the attractant:

[0012] Step 1: Dissolve ethyl benzoate in ethanol;

[0013] Step 2: dissolving the ethyl benzoate ethanol solution, glucose and potassium dihydrogen phosphate in water;

[0014] Step 3: Prepare the liquid attractant.

[0015] Furthermore, the concentration of ethanol is 95%, the concentration of ethyl benzoate is 0.1-0.5‰, the concentration of glucose is 1.0 - 5.0%, and the concentration of potassium dihydrogen phosphate is 0.1 - 1.0%.

[0016] Furthermore, the attractant is sprayed onto the surface of mulberry leaves, and the spraying is stopped when water drops fall from the leaf tips. The mulberry leaves are used to feed the bean sprouts, and the bean sprouts can eat and grow.

[0017] The above technical solution can achieve the following beneficial effects:

[0018] 1. The combination of attractants and mulberry leaves effectively expanded the host plant range of Bean Dan.

[0019] 2. It improves the guarantee for the large-scale consumption of bean curd and solves the problem that the low yield of a few legumes such as soybean, kudzu, locust, lentil, etc. cannot meet the large-scale consumption of bean curd.

[0020] 3. Spraying attractants on mulberry leaves to cultivate bean curd is also beneficial to increase production and income for mulberry farmers.

[0021] 4. It also avoids the problem of soybean leaves becoming dehydrated and limp quickly after being picked, thus realizing indoor cultivation of soybeans. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] Figure 1 This is an analysis chart of the growth of Bean Dandelion fed with mulberry leaves sprayed with different concentrations of ethyl benzoate solution for 8 days.

[0023] Figure 2 Growth analysis of mulberry leaf spray fed with different nutrient compound solutions for 8 days. DETAILED DESCRIPTION

[0024] The present invention will be further described below in conjunction with embodiments and drawings:

[0025] Determination of the main ingredient ethyl benzoate in bean attractant:

[0026] The plant leaves that bean sprouts like to eat the most are soybean leaves. First, the active substances with feeding function are separated and purified from soybean leaves. The active substance is sprayed on mulberry leaves and the bean sprouts can eat the mulberry leaves after the solvent evaporates as the activity tracking method.

[0027] The specific operation is as follows: pick soybean leaves, dry at 55℃, crush them, extract them with three times the volume of 80% ethanol at room temperature of 25℃ for three days each time, and evaporate the extract by vacuum rotary evaporation to obtain a black extract; extract the black extract with petroleum ether, and concentrate the extract at 52℃ to obtain a yellow-brown extract; apply the yellow-brown extract to a silica gel column, use three times the volume of ethyl acetate: petroleum ether system for gradient elution, collect the active components, and concentrate under vacuum to obtain a light yellow sample; separate the light yellow sample with a thin layer chromatography preparation plate to obtain the active component. Its structure was analyzed by nuclear magnetic resonance spectroscopy and confirmed to be ethyl benzoate.

[0028] Weigh 0.3 grams of ethyl benzoate, dissolve it in 3 ml of 95% ethanol solution, and then dilute it to 1000 ml with water. The final concentration of ethyl benzoate is 0.3‰. Then dilute it with distilled water to form a series of ethyl benzoate solutions of 0.1‰, 0.3‰, 1‰, 3‰, and 1%. Spray it on the surface of mulberry leaves and use it to feed bean larvae (50 bean larvae are fed at each concentration). After 8 days, the weight of bean larvae is measured as a basis for judging the growth condition. Feeding with bean leaves is used as a control. In order to facilitate the observation of the results, the bean larvae used are all fifth-instar bean larvae that have just molted; the results are shown in the figure. Figure 1 .Depend on Figure 1 It can be seen that the optimal concentration of ethyl benzoate is 0.3‰. In addition, during the study, it was found that 1% ethyl benzoate caused a slight agitation reaction in the bean mulberry, which affected the feeding and rest of the bean mulberry. But in general, there is still a certain gap between the growth of the bean mulberry and the bean leaf feeding, which shows that there is a certain difference in the nutritional components of mulberry leaves and bean leaves. It is necessary to add more nutrients and compound the attractant ingredients.

[0029] Compound single factor experiment: Different concentrations of glucose, milk, urea, foliar fertilizer (amino acid compound fertilizer, Shandong Baoyuan Biotechnology Co., Ltd.), and potassium dihydrogen phosphate were used as foliar supplementary nutrition, respectively, and mixed with 0.3‰ ethyl benzoate, sprayed on the surface of mulberry leaves, and used to feed bean worms (50 bean worms were fed at each concentration). After 8 days, the weight of bean worms was measured as a basis for judging the growth. The uncompounded 0.3‰ ethyl benzoate solution was used as a control. The results are shown in Figure 2 .

[0030] from Figure 2 It can be seen that 2% glucose and 3% potassium dihydrogen phosphate are compounded with 0.3‰ ethyl benzoate separately. Taking the growth of P. edulis, specifically the increase in insect body weight as the evaluation index, it is selected that potassium dihydrogen phosphate and glucose are compounded with ethyl benzoate with the best effect.

[0031] Compound orthogonal experiment: A three-factor three-level orthogonal experiment was carried out using ethyl benzoate (0.2‰, 0.3‰, 0.4‰), glucose (2%, 3%, 4%) and potassium dihydrogen phosphate (0.3%, 0.4%, 0.5%). The results are shown in the table below.

[0032]

[0033] The results of the range analysis showed that the attractant prepared by mixing 0.3‰ ethyl benzoate, 3% glucose and 0.4% potassium dihydrogen phosphate was the most effective and produced the highest weight gain for bean sprouts. Example

[0034] Accurately weigh 0.3 g of ethyl benzoate, dissolve it in 10 mL of 95% ethanol, and then pour it into 1 L of water. Then weigh 30 g of glucose and 4 g of potassium dihydrogen phosphate and add them to the mixture, stir until they are completely dissolved, and prepare an attractant solution. Spray the attractant on the surface of mulberry leaves until water drops fall from the tip of the leaves. Wait until the surface moisture is slightly dried, and use the mulberry leaves to feed the bean dan, which will eat and grow normally. Example

[0035] Accurately weigh 0.1 g of ethyl benzoate, dissolve it in 10 mL of 95% ethanol, and then pour it into 1 L of water. Then weigh 10 g of glucose and 1 g of potassium dihydrogen phosphate and add them to the mixture, stir until they are completely dissolved, and prepare an attractant solution. Spray the attractant on the surface of mulberry leaves until water drops fall from the tip of the leaves. Wait until the surface moisture is slightly dried, and use the mulberry leaves to feed the bean dan, which will eat and grow normally. Example

[0036] Accurately weigh 0.5 g of ethyl benzoate, dissolve it in 10 mL of 95% ethanol, and then pour it into 1 L of water. Then weigh 50 g of glucose and 10 g of potassium dihydrogen phosphate and add them to the mixture, stir until they are completely dissolved, and prepare an attractant solution. Spray the attractant on the surface of mulberry leaves until water drops fall from the tip of the leaves. Wait until the surface moisture is slightly dried, and use the mulberry leaves to feed the bean dan, which will eat and grow normally.

[0037] The above description of the disclosed embodiments enables one skilled in the art to implement or use the present invention. Various modifications to these embodiments will be apparent to one skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the present invention. Therefore, the present invention will not be limited to the embodiments shown herein, but rather to the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. A bean curd attractant, characterized in that: The ingredient of the attractant is ethyl benzoate; glucose and potassium dihydrogen phosphate are also added to the ingredients of the attractant; Preparation method of attractant: Step 1: Dissolve ethyl benzoate in ethanol with a concentration of 95%. Step 2: dissolving the ethyl benzoate ethanol solution, glucose and potassium dihydrogen phosphate in water; Step 3: obtain a liquid attractant, wherein the concentration of ethyl benzoate in the liquid attractant is 0.3‰, the concentration of potassium dihydrogen phosphate is 0.4%, and the concentration of glucose is 3%; The attractant is sprayed onto the surface of mulberry leaves, and the spraying is stopped when water drops are confirmed to fall from the leaf tips. The mulberry leaves are used to feed the bean sprouts, and the bean sprouts can eat and grow.

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