A method for preparing platelet-rich plasma

Abstract

The application provides a platelet-rich plasma preparation method, comprising: a centrifugal assembly with an inner cavity; and a suction assembly sleeved in the inner cavity of the centrifugal assembly; wherein the centrifugal assembly comprises: a centrifugal tube; a centrifugal tube sealing piston arranged in the centrifugal tube; and a centrifugal tube suction rod connected with the centrifugal tube sealing piston through a centrifugal tube piston connecting piece; and the suction assembly comprises: an inner suction tube; an inner suction tube sealing piston arranged in the inner suction tube; and an inner suction tube core rod connected with one end of the inner suction tube sealing piston. The platelet-rich plasma preparation method provided by the application is integrated with equipment, convenient to operate, and can effectively improve the stability, precision and concentration of platelet-rich plasma.

Description

Technical Field

[0001] This invention relates to the field of medical device technology, and in particular to a method for preparing platelet-rich plasma. Background Technology

[0002] Platelet-rich plasma (PRP) is a concentrated platelet solution obtained by centrifuging whole blood from humans or animals. It contains high concentrations of platelets, leukocytes, and fibrin. Once activated, platelets secrete various growth factors, including leukocytes which prevent infection and fibrin which helps build the three-dimensional structures needed for tissue repair. However, the difficulty in preparing PRP at the time limited its widespread clinical application.

[0003] In existing technologies, platelet-rich plasma (PRP) preparation typically employs methods such as single-stage centrifugation, double-stage centrifugation, triple-stage centrifugation, and equipment preparation. Among these, double-stage centrifugation yields the highest PRP extraction rate and is the most widely used in clinical practice.

[0004] While it's currently possible to prepare PRP with relatively simple components and a concentration meeting the standard (0), the preparation technology is not yet fully mature. The two-stage centrifugation method, which is widely recommended, is complex, lengthy, and carries the risk of product contamination. Furthermore, it suffers from drawbacks such as low platelet concentration and undisclosed hemoglobin concentration in PRP, potentially resulting in high platelet cell concentration but low purity. Moreover, the expensive PRP equipment currently available significantly limits its clinical application and promotion. Summary of the Invention

[0005] This invention provides an apparatus and method for preparing platelet-rich plasma (PRP) to solve the problems of complex PRP preparation processes, low purity, and instrument dispersion.

[0006] To solve the above-mentioned technical problems, the technical solution of the present invention is as follows:

[0007] A platelet-rich plasma preparation device, comprising:

[0008] Centrifuge assembly with an internal cavity;

[0009] An extraction component fitted inside the centrifugal assembly;

[0010] The centrifugation assembly includes:

[0011] Centrifuge tubes;

[0012] A centrifuge tube sealing piston is installed inside the centrifuge tube;

[0013] Centrifuge tube pull rod connected to the centrifuge tube sealing piston via centrifuge tube piston connector;

[0014] The extraction component includes:

[0015] Inner straw;

[0016] The inner suction tube is equipped with an inner suction tube sealing piston;

[0017] One end of the inner suction tube sealing piston is connected to the inner suction tube core rod.

[0018] Optionally, the centrifuge tube includes a tube body and a cone head;

[0019] One end of the cone is fixedly connected to the tube body, and the other end is fixedly connected to a first Luer connector.

[0020] The tube body is provided with a first double-wing handle at one end.

[0021] Optionally, the inner wall of the cone is provided with multiple flow guide grooves;

[0022] A groove is provided at the connection between the cone and the first Luer connector, the groove having an arc of 3 to 8 degrees, and one end of the guide groove is connected to the groove;

[0023] The angle between the sidewall of the cone and the central axis of the groove is 35 to 55 degrees.

[0024] Optionally, the centrifuge tube piston connector is provided with an inner sealing cap, and a second Luer connector and a third Luer connector are respectively provided at both ends of the inner sealing cap;

[0025] The second Luer connector and the third Luer connector have opposite thread directions.

[0026] Optionally, the centrifuge tube sealing piston has a cylindrical structure, the first end of the centrifuge tube sealing piston has a cavity structure, and the inner wall of the cavity of the centrifuge tube sealing piston is provided with an internal thread, which is connected to the centrifuge tube piston connector through the internal thread.

[0027] The second end of the centrifuge tube sealing piston has a concave structure with an arc of 120 to 140 degrees, and a through hole is provided at the center of the concave structure.

[0028] Optionally, a fourth Luer connector is provided at one end of the inner suction tube;

[0029] The other end is provided with a third double-wing handle, and the third double-wing handle is provided with a decorative piece;

[0030] The inner tube has a first scale mark on its outer wall.

[0031] Optionally, a second scale mark is provided on the outer wall of the tube.

[0032] Optionally, the platelet-rich plasma preparation device further includes an upper cap detachably connected to the cone of the centrifuge tube, which seals the centrifuge tube and keeps it upright.

[0033] Optionally, the platelet-rich plasma preparation device further includes an inner cap locking rod that is detachably connected to the inner cap.

[0034] The present invention also provides a method for preparing platelet-rich plasma, applied to the platelet-rich plasma preparation apparatus described above, the method comprising:

[0035] Remove the top cap of the centrifuge assembly, install a blood collection needle at the end of the centrifuge tube, draw blood, install the top cap and remove the centrifuge tube rod, and place the entire centrifuge tube into the centrifuge to centrifuge, causing the blood to separate into layers: the upper layer is the plasma layer, the middle layer is the platelet layer, and the lower layer is the red blood cell layer.

[0036] Remove the centrifuge tube from the centrifuge, lock the centrifuge tube lever, remove the top cap, press down the two wings of the centrifuge tube lever, and slowly press down to the liquid level line to discharge the lower layer of red blood cells from the centrifuge tube into the relevant container. Lock the top cap again and remove the centrifuge tube lever. Place the entire centrifuge tube into the centrifuge for centrifugation to separate the plasma into layers, with the upper layer being anemic platelet plasma and the lower layer being platelet-rich plasma.

[0037] Remove the centrifuge tube from the centrifuge, connect the centrifuge tube pull rod to the centrifuge tube, and remove the inner cap by using the inner cap locking rod; connect the inner pipette to the centrifuge tube piston connector, press down the two wings of the centrifuge tube pull rod, and slowly press down. Under the pressure, the inner pipette core rod rises in the opposite direction, allowing the anemic platelet plasma to completely enter the inner pipette. The remaining liquid is the required platelet-rich plasma.

[0038] The above-described solution of the present invention has at least the following beneficial effects:

[0039] A platelet-rich plasma (PRP) preparation device of the present invention includes: a centrifuge assembly with an inner cavity; and an extraction assembly fitted inside the inner cavity of the centrifuge assembly; wherein the centrifuge assembly includes: a centrifuge tube; a centrifuge tube sealing piston disposed inside the centrifuge tube; and a centrifuge tube extraction rod connected to the centrifuge tube sealing piston via a centrifuge tube piston connector; the extraction assembly includes: an inner pipette; an inner pipette sealing piston disposed inside the inner pipette; and an inner pipette core rod connected to one end of the inner pipette sealing piston. The PRP preparation device provided by the present invention is an integrated instrument, easy to operate, and can effectively improve the stability, accuracy, and concentration of PRP. Attached Figure Description

[0040] Figure 1This is a perspective view of the platelet-rich plasma preparation apparatus provided in an embodiment of the present invention;

[0041] Figure 2 This is an exploded view of the platelet-rich plasma preparation apparatus provided in an embodiment of the present invention;

[0042] Figure 3 This is a schematic diagram of the structure of the centrifuge assembly provided in an embodiment of the present invention;

[0043] Figure 4 This is a schematic diagram of the extraction component provided in an embodiment of the present invention;

[0044] Figure 5 This is a schematic diagram of the structure of a centrifuge tube provided in an embodiment of the present invention;

[0045] Figure 6 This is a schematic diagram of the centrifuge tube sealing piston provided in an embodiment of the present invention;

[0046] Figure 7 This is a cross-sectional view of the centrifuge tube sealing piston provided in an embodiment of the present invention;

[0047] Figure 8 This is a perspective view of the centrifuge tube sealing piston provided in an embodiment of the present invention;

[0048] Figure 9 This is a schematic diagram of the centrifuge tube piston connector provided in an embodiment of the present invention;

[0049] Figure 10 This is a perspective view of the centrifuge tube piston connector provided in an embodiment of the present invention;

[0050] Figure 11 This is a cross-sectional view of the centrifuge tube piston connector provided in an embodiment of the present invention;

[0051] Figure 12 This is a schematic diagram of the inner cap structure provided in an embodiment of the present invention.

[0052] Figure 13 This is a cross-sectional view of the inner cap provided in an embodiment of the present invention;

[0053] Figure 14 This is a schematic diagram of the centrifuge tube ejector provided in an embodiment of the present invention;

[0054] Figure 15 This is a schematic diagram of the inner cap locking rod provided in an embodiment of the present invention;

[0055] Figure 16 This is a schematic diagram of the inner suction tube provided in an embodiment of the present invention;

[0056] Figure 17This is a schematic diagram of the structure of the decorative piece provided in an embodiment of the present invention;

[0057] Figure 18 This is a schematic diagram of the structure of the inner suction tube sealing piston provided in an embodiment of the present invention;

[0058] Figure 19 This is a schematic diagram of the inner suction tube core rod provided in an embodiment of the present invention;

[0059] Figure 20 This is a cross-sectional view of the guide channel provided in an embodiment of the present invention;

[0060] Figure 21 This is a top view of the guide channel provided in an embodiment of the present invention;

[0061] Figure 22 This is a schematic diagram of the upper cap structure provided in an embodiment of the present invention.

[0062] Explanation of reference numerals in the attached figures:

[0063] 1. Centrifuge assembly; 11. Centrifuge tube; 111. Tube body; 112. Cone; 113. First Luer connector; 114. Flow guide groove; 115. Groove; 116. First double-wing handle; 117. Second graduation mark; 12. Centrifuge tube sealing piston; 121. Internal thread; 122. Second through hole; 13. Centrifuge tube piston connector; 131. First thread; 132. Second thread; 133. First through hole; 4. Centrifuge tube pull rod; 141. Second double-wing handle; 15. Inner cap; 151. Second Luer connector; 152. Third Luer connector; 2. Extraction assembly; 21. Inner suction tube; 211. Fourth Luer connector; 212. Third double-wing handle; 213. First scale mark; 22. Inner suction tube sealing piston; 23. Inner suction tube core rod; 24. Decorative piece; 3. Upper cap; 4. Inner cap locking rod; 41. Third thread. Detailed Implementation

[0064] Exemplary embodiments of the present disclosure will now be described in more detail with reference to the accompanying drawings. While exemplary embodiments of the present disclosure are shown in the drawings, it should be understood that the present disclosure may be implemented in various forms and should not be limited to the embodiments set forth herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete, and will fully convey the scope of the disclosure to those skilled in the art.

[0065] like Figures 1 to 4 As shown, an embodiment of the present invention provides a platelet-rich plasma preparation apparatus, comprising:

[0066] Centrifuge assembly 1 with an inner cavity;

[0067] Extraction component 2 is fitted inside the centrifugal assembly 1;

[0068] The centrifugation component 1 includes:

[0069] Centrifuge tube 11;

[0070] Centrifuge tube sealing piston 12 is disposed inside the centrifuge tube 11;

[0071] Centrifuge tube pull rod 14 is connected to centrifuge tube sealing piston 12 via centrifuge tube piston connector 13;

[0072] The extraction component 2 includes:

[0073] Inner straw 21;

[0074] An inner suction pipe sealing piston 22 is provided inside the inner suction pipe 21;

[0075] One end of the inner suction tube sealing piston 22 is connected to the inner suction tube core rod 23.

[0076] In this embodiment, the centrifugation component 1 and the extraction component 2 are used in conjunction to centrifuge and separate blood using the centrifugation tube 11 and the inner suction tube 21. This allows the collected fresh blood to be centrifuged and separated directly without being transferred to other containers, which is convenient, quick, reduces the error rate, shortens the operation time, reduces contamination, better preserves platelets, and facilitates medical staff to quickly extract platelet-rich plasma.

[0077] In an optional embodiment of the present invention, such as Figure 5 As shown, the centrifuge tube 11 includes a tube body 111 and a cone head 112;

[0078] One end of the cone 112 is fixedly connected to the tube 111, and the other end is fixedly connected to a first Luer connector 113.

[0079] One end of the tube 111 is provided with a first double-wing handle 116, which facilitates hooking or holding the centrifuge tube 11.

[0080] In an optional embodiment of the present invention, such as Figure 20 and Figure 21 As shown, the inner wall of the cone 112 is provided with a plurality of guide grooves 114;

[0081] A groove 115 is provided at the connection between the cone 112 and the first Luer connector 113. The groove 115 has an arc of 3 to 8 degrees. One end of the guide groove 114 is connected to the groove 115.

[0082] The angle between the sidewall of the cone 112 and the central axis of the groove 115 is 35 to 55 degrees.

[0083] In this embodiment, the guide channel 114 is provided with 6 to 8 channels, which converge towards the center at the groove 115. The arc of the groove 115 is set to 3 to 8 degrees, and the angle between the side wall of the cone 112 and the central axis of the groove 115 is set to 35 to 55 degrees, which can make the liquid flow more easily and be more precise during centrifugation and operation.

[0084] In an optional embodiment of the present invention, such as Figure 9 and Figure 11 As shown, the centrifuge tube piston connector 13 has a first thread 131 at the first end and a second thread 132 at the second end; wherein, the first thread 131 is a horizontal thread and the second thread 132 is a helical thread.

[0085] The centrifuge tube piston connector 13 has a second through hole 122 at its center. The angle between the side wall of the second through hole 122 and the central axis of the through hole is 8 degrees, which allows it to communicate with the second through hole 122 of the centrifuge tube sealing piston 12.

[0086] In an optional embodiment of the present invention, such as Figure 14 As shown, the centrifuge tube pull rod 14 has a hollowed-out groove on its tube wall;

[0087] The centrifuge tube pull rod 14 has a through hole at its first end, and the inner cap 15 can pass through the through hole and connect with the centrifuge tube pull rod 14 and the centrifuge tube piston connector 13.

[0088] The centrifuge tube pull rod 14 is also provided with a thread at the first end, which can be connected to the centrifuge tube piston connector 13.

[0089] The centrifuge tube pull rod 14 is provided with a second double-wing handle 141 at its second end.

[0090] In an optional embodiment of the present invention, such as Figures 12 to 13 As shown, the centrifuge tube piston connector 13 is provided with an inner cap 15, and the inner cap 15 is provided with a second Luer connector 151 and a third Luer connector 152 at both ends.

[0091] The second Luer connector 151 and the third Luer connector 152 have opposite thread directions.

[0092] In this embodiment, the inner cap 15 is provided with a second Luer connector 151 and a third Luer connector 152 at both ends. The threads of the second Luer connector 151 and the third Luer connector 152 are opposite and are respectively connected to the centrifuge tube piston connector 13 and the centrifuge tube pull rod 14 to improve the sealing effect inside the centrifuge tube.

[0093] The Luer connector on the inner cap 15 can cooperate with the inner cap locking rod 4 to disassemble the inner cap 15.

[0094] In an optional embodiment of the present invention, such as Figures 6 to 8 As shown, the centrifuge tube sealing piston 12 has a cylindrical structure, and the first end of the centrifuge tube sealing piston 12 has a cavity structure. The inner wall of the cavity of the centrifuge tube sealing piston 12 is provided with an internal thread 121, which is connected to the centrifuge tube piston connector 13 through the internal thread 121.

[0095] The second end of the centrifuge tube sealing piston 12 has a concave structure with an arc of 120 to 140 degrees. A second through hole 122 is provided at the center of the concave structure. The angle between the side wall of the second through hole 122 and the central axis of the through hole is 8 degrees.

[0096] In this embodiment, the concave structure at the bottom of the centrifuge tube sealing piston 12 can increase the pressure of the piston on the inside of the tube, causing the liquid to gather and flow from the peripheral wall to the center during the movement of the centrifuge tube sealing piston 12.

[0097] In an optional embodiment of the present invention, such as Figure 16 As shown, a fourth Luer connector 211 is provided at one end of the inner suction tube 21;

[0098] The other end is provided with a double-wing handle 212, and the double-wing handle 212 is provided with a decorative piece 24;

[0099] The inner suction tube 21 has a first scale mark 213 on its outer wall.

[0100] In this embodiment, a fourth Luer connector 211 is provided at one end of the inner suction tube 21 to facilitate connection with the centrifuge tube piston connector 13;

[0101] The dual-wing handle 212 allows for convenient pressing of the inner suction tube 21;

[0102] like Figure 17 As shown, the surface of the decorative piece 24 can be coated with a color that distinguishes it from other structures, making it easy to differentiate components during use and preventing operational errors.

[0103] In an optional embodiment of the present invention, a second scale mark 117 is provided on the outer wall of the tube 111 to clearly identify the amount of blood drawn.

[0104] In an optional embodiment of the present invention, such as Figure 22 As shown, the platelet-rich plasma preparation device further includes an upper cap 3 detachably connected to the cone 112 of the centrifuge tube 11. The upper cap 3 can seal the centrifuge tube 11 and allow the centrifuge tube 11 to be placed vertically.

[0105] In an optional embodiment of the present invention, such as Figure 15 As shown, the platelet-rich plasma preparation device further includes an inner cap locking rod 4 that is detachably connected to the inner cap 15.

[0106] In this embodiment, the end of the inner cap locking rod 4 is provided with a third thread 41, which can extend into the centrifuge tube extraction rod 14. The inner cap 15 is disassembled by matching the third thread 41 with the Luer connector of the inner cap 15, so that the centrifuge assembly 1 and the extraction assembly 2 can be connected, which can completely avoid contamination caused during disassembly and assembly.

[0107] The present invention also provides a method for preparing platelet-rich plasma, applied to the platelet-rich plasma preparation apparatus described above, the method comprising:

[0108] Step 1: Remove the upper cap 3 of the centrifuge assembly 1, install a blood collection needle at the end of the centrifuge tube 11, draw blood, install the upper cap 3 and remove the centrifuge tube pull rod 14, and put the centrifuge tube 11 into the centrifuge to centrifuge, so that the blood is divided into layers: the upper layer is the plasma layer, the middle layer is the platelet layer, and the lower layer is the red blood cell layer.

[0109] Step 2: Remove the centrifuge tube 11 from the centrifuge, lock the centrifuge tube lever 15, remove the upper cap 3, press down the two wings of the centrifuge tube lever 14, and slowly press down to the liquid level line to discharge the lower layer of red blood cells from the centrifuge tube 11 into the relevant container. Lock the upper cap 3 again, remove the centrifuge tube lever 14, and put the centrifuge tube 11 into the centrifuge to centrifuge, so that the plasma is divided into layers, with the upper layer being anemic platelet plasma and the lower layer being platelet-rich plasma.

[0110] Step 3: Remove the centrifuge tube 11 from the centrifuge, connect the centrifuge tube lever 14 to the centrifuge tube 11, and remove the inner cap 15 by using the inner cap locking lever 4; connect the inner pipette 21 to the centrifuge tube piston connector 13, press down the two wings of the centrifuge tube lever 14, and slowly press down. Under the pressure, the inner pipette core rod 23 rises in the opposite direction, allowing the anemic platelet plasma to completely enter the inner pipette 21. The remaining liquid is the required platelet-rich plasma.

[0111] In an optional embodiment of the present invention, step 1 may specifically include:

[0112] Step 11: Check the integrity of the packaging to confirm that all components are complete and undamaged, and open the package;

[0113] Step 12: Rotate the upper cap 3 of the centrifuge assembly 1 counterclockwise to remove it, install the blood collection needle at the Luer connector of the centrifuge tube 11, draw blood to the nominal mark, then rotate the upper cap 3 clockwise to lock it, and then rotate it counterclockwise to remove the centrifuge tube pull rod 14.

[0114] Step 13: After completing the above operations, place the centrifuge tube 11 into the centrifuge and centrifuge at 1500G for 10 minutes to separate the blood into layers: the upper layer is the plasma layer, the middle layer is the platelet layer, and the lower layer is the red blood cell layer.

[0115] Step 14: Take out the extraction component 1, hold the inner suction tube 21 with your left hand, and hold the inner suction tube core rod 23 with your right thumb and index finger and pull it open repeatedly 2-3 times.

[0116] In an optional embodiment of the present invention, step 2 may specifically include:

[0117] Step 21: Gently and vertically remove centrifuge tube 11. Rotate centrifuge tube lever 14 clockwise to lock it, and rotate counterclockwise to remove the upper cap 3. Place your index fingers under the wings of centrifuge tube 11 and clamp them to keep it vertical. Press down on the wings of centrifuge tube lever 14 with your thumbs and slowly press down to the liquid level line to discharge the red blood cells from centrifuge tube 11 into the relevant container. Rotate the upper cap 3 clockwise to lock it again, and then rotate counterclockwise to remove centrifuge tube lever 14.

[0118] Step 22: Place the centrifuge tube 11 back into the centrifuge and centrifuge at 1500G for 10 minutes to separate the plasma into layers: the upper layer is the anemic platelet plasma layer and the lower layer is the platelet-rich plasma layer.

[0119] In an optional embodiment of the present invention, step 3 may specifically include:

[0120] Step 31: Gently and vertically remove centrifuge tube 11, rotate centrifuge tube lever 14 clockwise to lock it, and use inner cap locking lever 4 to rotate counterclockwise to remove inner cap 15.

[0121] Step 32: Connect the Luer connector at one end of the inner pipette 21 to the Luer connector of the centrifuge tube piston connector 13, and rotate clockwise to lock it. Place your index fingers under the wings of the centrifuge tube 11 and clamp them to keep them vertical. Press down on the wings of the centrifuge tube lever 14 with your thumbs and slowly press down. Under the pressure, the inner pipette core rod 23 rises in the opposite direction until the anemic platelet plasma is completely inside the inner pipette 21. Hold the inner pipette core rod 23 with your left hand and rotate the wings of the inner pipette 32 counterclockwise with your right hand to remove the extraction component 2.

[0122] Step 33: Take out a new set of extraction components 2, hold the inner suction tube 21 with your left hand and hold the inner suction tube core rod 23 with your right thumb and index finger and pull it open repeatedly 2-3 times. Connect the Luer connector at one end of the inner suction tube 21 to the Luer connector of the centrifuge tube piston connector 13 and rotate clockwise to lock it.

[0123] Step 34: Flip the device 180 degrees so that the upper cap 3 of the centrifugation assembly 1 is facing upward. Rotate the upper cap 3 counterclockwise to remove it. Hold the centrifuge tube 11 with your left hand and hold the inner pipette rod 23 with your right thumb and forefinger to draw platelet-rich plasma from the centrifuge tube 11 into the extraction assembly 2. After installing the needle at the Luer connector at one end of the inner pipette 21, it can be used immediately at the treatment site.

[0124] It should be noted that this method is the same as the method described above. All implementations in the above device embodiments are applicable to the embodiments of this method and can achieve the same technical effect.

[0125] The above-mentioned platelet-rich plasma preparation apparatus and method of the present invention improve the convenience of platelet-rich plasma preparation, completely avoid contamination during the process, further improve the stability, accuracy and concentration of platelet-rich plasma preparation, solve various problems faced by platelet-rich plasma preparation technology in clinical application, better promote the clinical application of platelet-rich plasma, and benefit more patients.

[0126] The above description represents the preferred embodiments of the present invention. It should be noted that those skilled in the art can make various improvements and modifications without departing from the principles of the present invention, and these improvements and modifications should also be considered within the scope of protection of the present invention.

Claims

1. A method for preparing platelet-rich plasma, characterized in that, The platelet-rich plasma preparation device includes: a centrifuge assembly (1) with an inner cavity; and an extraction assembly (2) fitted inside the inner cavity of the centrifuge assembly (1). The centrifuge assembly (1) includes: a centrifuge tube (11); a centrifuge tube sealing piston (12) disposed inside the centrifuge tube (11); and a centrifuge tube rod (14) connected to the centrifuge tube sealing piston (12) via a centrifuge tube piston connector (13). The extraction assembly (2) includes: an inner suction tube (21); an inner suction tube sealing piston (22) is disposed inside the inner suction tube (21); one end of the inner suction tube sealing piston (22) is connected to an inner suction tube core rod (23); the centrifuge tube piston connector (13) has a first thread (131) at one end and a second thread (132) at the other end; wherein the first thread (131) is a horizontal thread and the second thread (132) is a helical thread; the centrifuge tube piston connector (131)... A first through hole (133) is provided at the center; an inner cap (15) is provided on the centrifuge tube piston connector (13), and a second Luer connector (151) and a third Luer connector (152) are respectively provided at both ends of the inner cap (15); the threads of the second Luer connector (151) and the third Luer connector (152) are opposite; a fourth Luer connector (211) is provided at one end of the inner suction tube (21); a third double-wing handle (212) is provided at the other end, and a decorative piece (24) is provided on the third double-wing handle (212); a first scale mark (213) is provided on the outer wall of the inner suction tube (21); an upper cap (3) is detachably connected to the cone (112) of the centrifuge tube (11), and the upper cap (3) seals the centrifuge tube (11) and makes the centrifuge tube (11) stand upright; an inner cap locking rod (4) is detachably connected to the inner cap (15), and the method includes: Remove the cap (3) from the centrifuge assembly (1), install a blood collection needle at the end of the centrifuge tube (11), draw blood, install the cap (3) and remove the centrifuge tube rod (14), put the centrifuge tube (11) into the centrifuge and centrifuge it to separate the blood into layers: the upper layer is the plasma layer, the middle layer is the platelet layer, and the lower layer is the red blood cell layer. Remove the centrifuge tube (11) from the centrifuge, lock the centrifuge tube lever (14), remove the upper cap (3), press down the two wings of the centrifuge tube lever (14), slowly press down to the liquid level line, discharge the lower layer of red blood cells from the centrifuge tube (11) into the relevant container, lock the upper cap (3) again, remove the centrifuge tube lever (14), put the centrifuge tube (11) into the centrifuge as a whole, centrifuge, so that the plasma is divided into layers, the upper layer is the anemic platelet plasma layer, and the lower layer is the platelet-rich plasma layer; Remove the centrifuge tube (11) from the centrifuge, connect the centrifuge tube lever (14) to the centrifuge tube (11), and remove the inner cap (15) by using the inner cap locking lever (4); connect the inner tube (21) to the centrifuge tube piston connector (13), press down the two wings of the centrifuge tube lever (14), and slowly press down. Under the pressure, the inner tube core rod (23) rises in the opposite direction, so that the anemic platelet plasma completely enters the inner tube (21), and the remaining liquid is the required platelet-rich plasma.

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