A microbial strain propagation apparatus

Abstract

The present application relates to the technical fields of microbial culture, and especially relates to a microbial strain expansion equipment, which comprises a plurality of strain storage cabinet assemblies arranged in a top-down stacking mode, a plurality of supporting bottom columns are installed at the bottom of each strain storage cabinet assembly, a plurality of plug-in holes matched with the supporting bottom columns on the strain storage cabinet assembly of the lower layer are respectively installed at the top of each strain storage cabinet assembly, a push-in air pipe group is respectively arranged at the center of the top of each strain storage cabinet assembly, and an air inlet linkage assembly is respectively arranged at the center of the bottom of each strain storage cabinet assembly. The microbial strain expansion equipment adopts a modular structure, each modular strain storage cabinet assembly can be used individually or used as an entire expansion assembly after combination, the combination and separation are relatively simple and fast, and the rapid connection of the interiors of the strain storage cabinet assemblies can be automatically realized after combination.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of microbial culture, in particular to a new technology and new equipment capable of realizing industrial expansion of microbial culture, and especially to a microbial strain expansion equipment. BACKGROUND

[0002] The sources of microbial strains are diversified, generally including isolation from nature (small amount of separation, and currently less than 1% of the total number of microorganisms isolated from soil), mutagenesis breeding (mutagenesis breeding is an important guarantee for stable production and high yield of industrial fermentation), and gene recombination (gene directed modification technology greatly accelerates the development process of biological products).

[0003] Among them, the cultivation of strains obtained by mutagenesis breeding is one of the important means of microbial expansion in industry. Generally, when expanding microbial strains, the strains need to be stored in an ultra-low temperature freezer, and the industrial strain processing technology is used to realize the preservation and expansion of the strains.

[0004] For example, a modular movable microbial expansion device is disclosed in patent application No. CN202222304720.X, which mainly includes a cabinet, a door plate hinged to one side of the cabinet, a roller provided at the bottom of the cabinet, a thermostat provided at the inside top of the cabinet, a switch provided at the top of the cabinet, a fixed slot provided on one side of the inner side wall of the cabinet, a fixed block fixedly connected to the other side of the inner side wall of the cabinet, an activity cavity opened in the inside of the fixed block, a spiral spring fixedly connected to the inside of the activity cavity, an activity plate movably connected to the inside of the activity cavity, an activity slot opened on one side of the activity plate, the spiral spring and the activity plate being fixedly connected, a supporting plate clamped by the activity slot and the fixed slot in the inside of the cabinet, an air inlet pipe provided at the top of one side of the cabinet, a filter screen provided in the inside of the air inlet pipe, and an air outlet hole provided at the bottom of the other side of the cabinet.

[0005] As can be seen from the above, the movable microbial expansion device is actually a culture cabinet structure. It can adjust the air inlet amount of the air inlet pipe through the cooperation between the air inlet pipe, the baffle, the screw rod and the movable hole, and is simple and fast to operate, which is conducive to making the microorganisms in a suitable growth environment and improving the efficiency of microbial expansion. It can be seen that the structure mainly uses the supporting plate structure to pull out and place during expansion, and each supporting plate is still placed in the inside of the cabinet, which can effectively realize the expansion of similar strains. However, it cannot realize the expansion of different strains, so there is a certain limitation in the actual multi-strain culture process, and the purpose of effective partitioning and joint control cannot be achieved.

[0006] To this end, the present application proposes a new technology and new equipment capable of realizing industrial expansion of microbial culture to better solve the problems existing in the prior art. SUMMARY

[0007] To solve one of the above technical problems, the present application employs the technical solution of a microbial strain expansion equipment, comprising a plurality of strain storage cabinet assemblies arranged in a top-down stacking manner, a plurality of support columns are installed at the bottom of each strain storage cabinet assembly, a plurality of plug-in holes matched with each support column on the strain storage cabinet assembly of the lower layer are installed at the top of each strain storage cabinet assembly, a push-in air pipe group is arranged at the center of the top of each strain storage cabinet assembly, and an air inlet linkage assembly is arranged at the center of the bottom of each strain storage cabinet assembly.

[0008] In any of the above solutions, it is preferred that a lateral air supply assembly is arranged at each side of each strain storage cabinet assembly.

[0009] In any of the above solutions, it is preferred that the strain storage cabinet assembly comprises a horizontal bacterial colony cabinet body, a plurality of layers of drawer-type culture trays are arranged inside the culture chamber of the horizontal bacterial colony cabinet body, and a double-door is installed on the cabinet body at the front of the culture chamber, which is used to realize the sealing of the inside of the culture chamber.

[0010] In any of the above solutions, it is preferred that the push-in air pipe group comprises a push-in pipe fixedly installed at the center of the top of the horizontal bacterial colony cabinet body, the inside of the push-in pipe is in communication with the inside of the culture chamber of the corresponding horizontal bacterial colony cabinet body, a first fixed ring and a second fixed ring are fixedly installed inside the pipe cavity of the push-in pipe from top to bottom, a push-in one-way rubber valve ball is installed in the pipe cavity between the first fixed ring and the second fixed ring, the top of the push-in one-way rubber valve ball is sealed against the inner ring of the first fixed ring, a first spring is fixed to the bottom of the push-in one-way rubber valve ball, the bottom of the first spring is fixedly connected with the top of the second fixed ring, a lower vertical push-in column is fixedly installed at the center of the top of the push-in one-way rubber valve ball, the top of the lower vertical push-in column protrudes out of the upper part of the push-in pipe, and the outer diameter of the lower vertical push-in column is smaller than the inner diameter of the inner ring of the first fixed ring.

[0011] Preferably in any of the above solutions, the air inlet linkage assembly comprises a sealing sleeve pressing pipe fixedly installed at the center of the bottom of the horizontal bacterial population cabinet body, the inside of the sealing sleeve pressing pipe communicates with the inside of the culture bin of the corresponding horizontal bacterial population cabinet body, a third fixed ring and a fourth fixed ring are fixedly installed in the tube cavity of the sealing sleeve pressing pipe from top to bottom in sequence, a passive one-way rubber valve ball is arranged in the tube cavity between the third fixed ring and the fourth fixed ring, the bottom of the passive one-way rubber valve ball sealingly abuts against the inner ring of the fourth fixed ring, a second spring is fixedly connected to the top of the passive one-way rubber valve ball, the top of the second spring abuts against the bottom of the third fixed ring, the inner ring of the fourth fixed ring is coaxially arranged with the lower vertical pushing and pressing column and the inner diameter thereof matches the outer diameter of the lower vertical pushing and pressing column.

[0012] Preferably in any of the above solutions, the lateral ventilation assembly comprises a plurality of lateral ventilation pipes with control valves, each lateral ventilation pipe is used for introducing gas into the inside of the culture bin of the corresponding horizontal bacterial population cabinet body or discharging gas to the outside.

[0013] Preferably in any of the above solutions, a thermometer, a hygrometer and a pressure gauge for detecting the temperature, humidity and pressure inside the culture bin are further installed on the side wall of the horizontal bacterial population cabinet body.

[0014] Preferably in any of the above solutions, a transparent glass observation window for observing the inside of the corresponding culture bin is arranged on each of the double doors.

[0015] Compared with the prior art, the present application has the following beneficial effects:

[0016] 1. The microbial strain expansion equipment adopts a modular structure, and each modular strain storage cabinet assembly can be used individually or combined as an entire expansion assembly, the combination and separation are relatively simple and fast, and the combination can automatically realize the rapid connection of the interiors of the strain storage cabinet assemblies, and the overall structure assembly and separation are simpler and faster.

[0017] 2. After the expansion equipment is combined, the matched pushing and pressing air inlet pipe group and air inlet linkage assembly can be quickly plugged and matched to realize bidirectional conduction; meanwhile, after the assemblies of the expansion equipment are separated, the pushing and pressing air inlet pipe group and air inlet linkage assembly can be quickly plugged, so as to ensure the quick control of the opening and closing of the pipe opening after the strain storage cabinet assembly is disassembled and separated.

[0018] 3. When the strain storage cabinet assembly is combined to form the expansion device, the lateral ventilation assembly on both sides of each strain storage cabinet assembly can be used to introduce hot air of corresponding temperature and material, and meanwhile, the intercommunication push-in air pipe group and the air inlet linkage assembly are used to realize the intercommunication and flow guide of each culture cabinet stacked in up and down, to ensure the intercommunication of air flow in each culture cabinet and improve the effective control of internal environment. BRIEF DESCRIPTION OF DRAWINGS

[0019] In order to more clearly illustrate the technical solutions of the present application, the following will briefly introduce the drawings needed in the description of the specific embodiments or prior art. In all the drawings, similar elements or components are generally identified by similar reference numerals. In the drawings, each element or component is not necessarily drawn according to the actual proportion.

[0020] Fig. 1 The figure is a structural schematic diagram of the present application.

[0021] Fig. 2 The figure is a structural schematic diagram of the present application after combination of each strain storage cabinet assembly.

[0022] Fig. 3 The figure is a structural schematic diagram of the present application after combination of each strain storage cabinet assembly.

[0023] In the figure, 1 is a strain storage cabinet assembly; 2 is a supporting bottom column; 3 is a horizontal strain group cabinet body; 301 is a culture cabinet; 302 is a drawer type culture tray; 303 is a double door; 4 is a push-in pipe; 5 is a first fixed ring; 6 is a second fixed ring; 7 is a push-in one-way rubber valve ball; 8 is a first spring; 9 is a lower vertical push-in column; 10 is a sealing sleeve pressure pipe; 11 is a third fixed ring; 12 is a fourth fixed ring; 13 is a passive one-way rubber valve ball; 14 is a second spring; 15 is a lateral ventilation pipe; 16 is a control valve; 17 is a thermometer; 18 is a hygrometer; 19 is a pressure gauge; 20 is a transparent glass observation window; and 21 is a plug-in hole. DETAILED DESCRIPTION

[0024] The embodiments of the technical solutions of the present application will be described in detail below with reference to the drawings. The following embodiments are only used to more clearly illustrate the technical solutions of the present application, and therefore only serve as examples, but cannot limit the protection scope of the present application. The specific structure of the present application is shown in the drawings. Figs. 1-3

[0025] ​Embodiment 1: a microbial strain expansion equipment, comprising a plurality of strain storage cabinet assemblies 1 arranged in a top-down stacking manner, a plurality of supporting bottom columns 2 are installed at the bottom of each strain storage cabinet assembly 1, a plurality of plug-in holes 21 matched with each supporting bottom column 2 on the strain storage cabinet assembly 1 below are installed at the top of each strain storage cabinet assembly 1, a push-in air pipe group is arranged at the center of the top of each strain storage cabinet assembly 1, and an air inlet linkage assembly is arranged at the center of the bottom of each strain storage cabinet assembly 1. The microbial strain expansion equipment designed in the application can realize the overall cooperation of the stacked combination of the plurality of strain storage cabinet assemblies 1, that is, the rapid connection of the internal culture chambers 301, so as to ensure the connectivity of the internal culture chambers 301 and realize the rapid flow and conduction of hot air or air in the entire internal space when the air or hot air is subsequently introduced. In addition, the stability of mutual positioning of each strain storage cabinet assembly 1 is realized by the plug-in of the corresponding supporting bottom column 2 and the corresponding plug-in hole after the stacking of each strain storage cabinet assembly 1, so as to ensure the stability and firmness of the top-down stacking of each strain storage cabinet assembly 1. At the same time, the rapid cultivation of different strains can be realized when each strain storage cabinet assembly 1 is separated from each other, so as to ensure the diversity of strain cultivation.

[0026] In any of the above schemes, preferably, a lateral air supply assembly is arranged at both sides of each strain storage cabinet assembly 1. The lateral air supply assembly can introduce or discharge the required gas or hot air in the interior of a single strain storage cabinet assembly 1, so as to effectively serve the purpose of cultivating internal microbial flora.

[0027] In any of the above schemes, preferably, the strain storage cabinet assembly 1 comprises a horizontal microbial flora cabinet body 3, a plurality of drawer-type culture trays 302 are arranged in the culture chamber 301 of the horizontal microbial flora cabinet body 3, and a double-door 303 is installed on the front cabinet body of the culture chamber 301, which is used to realize the sealing of the interior of the culture chamber 301. The culture chamber 301 space in the strain storage cabinet assembly 1 can store multiple layers of trays, each tray can be used to cultivate microorganisms of the same strain, and the purpose of small-batch expansion of a single strain storage cabinet assembly 1 can be realized, and the double-door 303 arranged herein can realize the effect of internal sealing when closed.

[0028] In any of the above schemes, preferably, the push-inlet pipe set comprises a push pipe 4 fixedly installed at the top center of the horizontal bacterial flora cabinet body 3, the inside of the push pipe 4 is in communication with the inside of the culture bin 301 of the corresponding horizontal bacterial flora cabinet body 3, a first fixed ring 5 and a second fixed ring 6 are respectively fixedly installed in the tube cavity of the push pipe 4 from top to bottom, a push one-way rubber valve ball 7 is installed in the tube cavity between the first fixed ring 5 and the second fixed ring 6, the top of the push one-way rubber valve ball 7 is sealed against the inner ring of the first fixed ring 5, a first spring 8 is fixed to the bottom of the push one-way rubber valve ball 7, the bottom of the first spring 8 is fixedly connected with the top of the second fixed ring 6, a lower vertical push column 9 is fixedly installed at the center of the top of the push one-way rubber valve ball 7, the top of the lower vertical push column 9 protrudes to the upper part of the push pipe 4, and the outer diameter of the lower vertical push column 9 is smaller than the inner diameter of the inner ring of the first fixed ring 5. The whole push-inlet pipe set adopts a one-way conduction structure, that is, in the normal state when the horizontal bacterial flora cabinet body 3 is used alone, the push pipe 4 is blocked under the action of the corresponding push one-way rubber valve ball 7, only when the push one-way rubber valve ball 7 is pressed will the first spring 8 be compressed, so as to quickly open the inner ring of the first fixed ring 5, at this time the inside of the culture bin 301 is communicated with the outside, so as to facilitate the entry of external oxygen and air; the lower vertical push column 9 serves as an execution component and controls the corresponding push one-way rubber valve ball 7 to be pressed downward when pressed, so as to finally realize communication.

[0029] In any of the above schemes, preferably, the air inlet linkage assembly includes a sealing sleeve pressing pipe 10 fixedly installed at the center of the bottom of the horizontal bacterial flora cabinet body 3, the inside of the sealing sleeve pressing pipe 10 communicates with the inside of the culture bin 301 of the corresponding horizontal bacterial flora cabinet body 3, a third fixed ring 11 and a fourth fixed ring 12 are sequentially fixedly installed inside the lumen of the sealing sleeve pressing pipe 10 from top to bottom, a passive one-way rubber valve ball 13 is arranged in the lumen between the third fixed ring 11 and the fourth fixed ring 12, the bottom of the passive one-way rubber valve ball 13 sealingly abuts against the inner ring of the fourth fixed ring 12, a second spring 14 is fixedly connected to the top of the passive one-way rubber valve ball 13, the top of the second spring 14 abuts against the bottom of the third fixed ring 11, the inner ring of the fourth fixed ring 12 is coaxially arranged with the lower vertical push column 9 and its inner diameter matches the outer diameter of the lower vertical push column 9. In the normal natural state of the air inlet linkage assembly, the passive one-way rubber valve ball 13 is forced to abut against the fourth fixed ring 12 and block the inner ring thereof under the action of the second spring 14, so as to achieve the purpose of blocking the culture bin 301 inside the horizontal bacterial flora cabinet body 3. When the air inlet linkage assembly is stacked above the lower air inlet linkage assembly, the air inlet linkage assembly can press the lower vertical push column 9 inside the push pipe 4 at the top of the horizontal bacterial flora cabinet body 3 below. At this time, the lower vertical push column 9 is mainly pressed by the push one-way rubber valve ball 7 of the upper horizontal bacterial flora cabinet body 3, so as to abut against the lower vertical push column 9 below and push away the passive one-way rubber valve ball 13 above the lower vertical push column 9 and the push one-way rubber valve ball 7 below, so as to control the opening of the switch at the passive one-way rubber valve ball 13 and the push one-way rubber valve ball 7, and finally achieve the purpose of controlling the communication between the horizontal bacterial flora cabinet body 3 above and the horizontal bacterial flora cabinet body 3 below. Meanwhile, when the air inlet linkage assembly is cooperatively inserted with the push air inlet pipe group below, the sealing sleeve pressing pipe 10 can be sealingly sleeved on the outer sidewall of the push pipe 4, so as to ensure the sealing of the connected part.

[0030] In any of the above schemes, preferably, the lateral ventilation assembly includes a plurality of lateral ventilation pipes 15 with control valves 16, each of the lateral ventilation pipes 15 is used for introducing gas into the inside of the culture bin 301 of the corresponding horizontal bacterial flora cabinet body 3 or discharging gas to the outside. The lateral ventilation assembly is mainly used for realizing the ventilation between the inside and the outside of each bacterial strain storage bin assembly 1 when the bacterial strain storage bin assembly 1 is used as a single culture container, so as to ensure the normal ventilation in the inside of the bacterial strain storage bin assembly 1, provide the required gas and temperature for the growth of microorganisms in the inside, and preheat the gas at a suitable temperature according to the requirement when the gas is introduced.

[0031] Embodiment 2: A microbial strain expansion device, comprising a plurality of strain storage cabinet assemblies 1 arranged in a top-down stack, a plurality of support bottom columns 2 are installed at the bottom of each of the strain storage cabinet assemblies 1, a plurality of plug-in holes are respectively installed at the top of each of the strain storage cabinet assemblies 1 and matched with each of the support bottom columns 2 on the strain storage cabinet assembly 1 below, a push-in air pipe group is respectively arranged at the top center of each of the strain storage cabinet assemblies 1, and an air inlet linkage assembly is respectively arranged at the bottom center of each of the strain storage cabinet assemblies 1.

[0032] In any of the above solutions, it is preferred that a lateral air supply assembly is respectively arranged at both sides of each of the strain storage cabinet assemblies 1.

[0033] The lateral air supply assembly can introduce or exhaust the required gas or hot air inside a single strain storage cabinet assembly 1, which effectively serves the purpose of cultivating internal microbial flora.

[0034] In any of the above solutions, it is preferred that the strain storage cabinet assembly 1 comprises a horizontal microbial flora cabinet body 3, a plurality of layers of drawer-type cultivation trays 302 are arranged inside the cultivation chamber 301 of the horizontal microbial flora cabinet body 3, and a double-door 303 is installed on the front cabinet body of the cultivation chamber 301, which is used to realize the sealing of the inside of the cultivation chamber 301.

[0035] The cultivation chamber 301 space inside the strain storage cabinet assembly 1 can store multiple layers of trays, each of which can be used to cultivate microorganisms of the same strain, sequentially achieving the purpose of small-batch expansion of a single strain storage cabinet assembly 1, and the double-door 303 arranged here can achieve the effect of internal sealing when closed.

[0036] In any of the above solutions, it is preferred that the push-in air pipe group comprises a push-in pipe 4 fixedly installed at the top center of the horizontal microbial flora cabinet body 3, the inside of the push-in pipe 4 is in communication with the inside of the cultivation chamber 301 of the corresponding horizontal microbial flora cabinet body 3, a first fixed ring 5 and a second fixed ring 6 are respectively fixedly installed inside the pipe cavity of the push-in pipe 4 from top to bottom, a push-in one-way rubber valve ball 7 is installed in the pipe cavity between the first fixed ring 5 and the second fixed ring 6, the top of the push-in one-way rubber valve ball 7 is sealed against the inner ring of the first fixed ring 5, a first spring 8 is fixed to the bottom of the push-in one-way rubber valve ball 7, the bottom of the first spring 8 is fixedly connected with the top of the second fixed ring 6, a lower vertical push-in column 9 is fixedly installed at the top center of the push-in one-way rubber valve ball 7, the top of the lower vertical push-in column 9 extends to the upper part of the push-in pipe 4, and the outer diameter of the lower vertical push-in column 9 is smaller than the inner diameter of the inner ring of the first fixed ring 5.

[0037] The push-inlet pipe group as a whole adopts a one-way conduction structure, that is, in the normal state when the horizontal bacterial flora cabinet body 3 is used alone, the push pipe 4 is blocked under the action of the corresponding push one-way rubber valve ball 7, only when the push one-way rubber valve ball 7 is pressed, the first spring 8 is compressed, so as to quickly open the inner ring of the first fixed ring 5, at this time, the inside of the culture bin 301 is connected with the outside, so as to facilitate the entry of external oxygen and air; The lower vertical push column 9 arranged at the lower part is used as an execution component, which controls the corresponding push one-way rubber valve ball 7 to press downward when it is pressed, and finally realizes the connection.

[0038] In any of the above schemes, preferably, the air inlet linkage assembly includes a sealing sleeve pressing pipe 10 fixedly installed at the bottom center of the horizontal bacterial flora cabinet body 3, the inside of the sealing sleeve pressing pipe 10 is communicated with the inside of the culture bin 301 of the horizontal bacterial flora cabinet body 3, the third fixed ring 11 and the fourth fixed ring 12 are sequentially fixedly installed in the lumen of the sealing sleeve pressing pipe 10 from top to bottom, a passive one-way rubber valve ball 13 is arranged in the lumen between the third fixed ring 11 and the fourth fixed ring 12, the bottom of the passive one-way rubber valve ball 13 is sealed against the inner ring of the fourth fixed ring 12, the top of the passive one-way rubber valve ball 13 is fixedly connected with the second spring 14, the top of the second spring 14 abuts against the bottom of the third fixed ring 11, the inner ring of the fourth fixed ring 12 is coaxially arranged with the lower vertical push column 9, and the inner diameter of the fourth fixed ring 12 matches the outer diameter of the lower vertical push column 9.

[0039] In the normal natural state of the air inlet linkage assembly, the passive one-way rubber valve ball 13 is forced to abut against the fourth fixed ring 12 and block the inner ring of the fourth fixed ring 12 under the action of the second spring 14, so as to achieve the purpose of blocking the culture bin 301 inside the horizontal bacterial flora cabinet body 3, when the air inlet linkage assembly is stacked above the lower air inlet linkage assembly, the lower vertical push column 9 in the inside of the push pipe 4 at the top of the horizontal bacterial flora cabinet body 3 below can be pressed down, at this time, the lower vertical push column 9 is mainly pressed by the push one-way rubber valve ball 7 of the upper horizontal bacterial flora cabinet body 3 to press the passive one-way rubber valve ball 13, so as to abut against the lower vertical push column 9 below, at this time, the passive one-way rubber valve ball 13 above the lower vertical push column 9 and the push one-way rubber valve ball 7 below are pushed away, so as to control the opening of the switch at the push one-way rubber valve ball 7 and the passive one-way rubber valve ball 13, finally, the purpose of controlling the connection between the upper horizontal bacterial flora cabinet body 3 and the lower horizontal bacterial flora cabinet body 3 is achieved, at the same time, when the air inlet linkage assembly is inserted into the push-inlet pipe group below, the sealing sleeve pressing pipe 10 can be sealingly sleeved on the outer sidewall of the push pipe 4, so as to ensure the sealing of the connected part.

[0040] Preferably in any of the above solutions, the lateral ventilation assembly comprises a plurality of lateral ventilation pipes 15 with control valves 16, each of the lateral ventilation pipes 15 is used to introduce gas into or exhaust gas out of the inside of the corresponding culture bin 301 of the horizontal bacterial flora cabinet body 3.

[0041] The lateral ventilation assembly is mainly used to realize the ventilation of the inside and outside of each bacterial strain storage bin assembly 1 when it is used as a single culture container, to ensure the normal ventilation of the inside of the bacterial strain storage bin assembly 1, to provide the required gas and temperature for the growth of microorganisms, and to preheat the gas to a suitable temperature as needed when introducing the gas.

[0042] Preferably in any of the above solutions, a thermometer 17, a hygrometer 18 and a pressure gauge 19 are further installed on the side wall of the horizontal bacterial flora cabinet body 3 to detect the temperature, humidity and pressure inside the culture bin 301.

[0043] The thermometer 17, the hygrometer 18 and the pressure gauge 19 can more conveniently observe the growth environment of the microorganisms inside, and facilitate the regulation and adjustment as needed to ensure the rapid and stable growth of the microorganisms.

[0044] Preferably in any of the above solutions, a transparent glass observation window 20 is arranged on each of the double doors to observe the inside of the corresponding culture bin 301.

[0045] The transparent glass observation window 20 can facilitate the intuitive observation of the growth of the bacteria inside, so as to achieve the purpose of adjusting the temperature of the internal environment in real time according to the observation results.

[0046] Specific working principle: The microbial strain expansion equipment designed by the present application can realize the overall cooperation of the stacked combination of the plurality of bacterial strain storage bin assemblies 1, that is, the rapid connection of the inside culture bins 301, to ensure the connectivity of the inside culture bins 301, so that when air or hot air is introduced subsequently, the hot air or air can be rapidly guided and connected in the entire internal space.

[0047] After the stacking of each bacterial strain storage bin assembly 1, the corresponding each support column 2 and the corresponding plug-in hole are plugged in to realize the stability of mutual positioning, to ensure the stability and firmness of the stacking of each bacterial strain storage bin assembly 1 from top to bottom.

[0048] When each bacterial strain storage bin assembly 1 is separated from each other, the rapid cultivation of different bacterial strains can be realized, to ensure the diversity of bacterial strain cultivation.

[0049] The culture bin 301 space inside the strain storage cabinet assembly 1 can store multiple layers of trays, each tray can be used to culture microorganisms of the same strain, and the single strain storage cabinet assembly 1 can achieve the purpose of small-batch expansion. The double-leaf door 303 can achieve the effect of internal sealing when closed.

[0050] In the normal state of the horizontal bacterial group cabinet body 3, the push pipe 4 is blocked by the corresponding push one-way rubber valve ball 7. Only when the push one-way rubber valve ball 7 is pressed will the first spring 8 be compressed, thereby quickly opening the inner ring of the first fixed ring 5, and the culture bin 301 is connected to the outside, so that external oxygen and air can enter. The lower vertical push column 9 is used as an execution component and controls the corresponding push one-way rubber valve ball 7 to press down when pressed, thereby achieving connection. In the normal natural state of the air inlet linkage assembly, the passive one-way rubber valve ball 13 is forced against the fourth fixed ring 12 and blocks the inner ring, thereby achieving the purpose of blocking the culture bin 301 inside the horizontal bacterial group cabinet body 3. When the air inlet linkage assembly is stacked above the lower air inlet linkage assembly, the air inlet linkage assembly can press the lower vertical push column 9 inside the push pipe 4 at the top of the horizontal bacterial group cabinet body 3 below. At this time, the lower vertical push column 9 is pressed mainly by the push one-way rubber valve ball 7 of the upper horizontal bacterial group cabinet body 3 pushing the passive one-way rubber valve ball 13, thereby achieving the purpose of abutting the lower vertical push column 9 below. At this time, the passive one-way rubber valve ball 13 above the lower vertical push column 9 and the push one-way rubber valve ball 7 below are pushed away, thereby achieving the purpose of controlling the switch at the push one-way rubber valve ball 7 and the passive one-way rubber valve ball 13 to open. Finally, the purpose of controlling the connection between the upper horizontal bacterial group cabinet body 3 and the lower horizontal bacterial group cabinet body 3 is achieved. When the air inlet linkage assembly and the push air pipe group below are connected, the sealing sleeve pipe 10 can be used to seal the movable sleeve on the outer wall of the push pipe 4, thereby ensuring the sealing of the connected part.

[0051] The microbial strain expansion equipment adopts a modular structure, and each modular strain storage cabinet assembly 1 can be used individually or combined as a whole expansion assembly, and the combination and separation is relatively simple and fast, and after combination, the rapid connection of the inside of each strain storage cabinet assembly 1 can be automatically realized, and the overall structure assembly and separation is simpler and faster; after the expansion equipment is combined, the matched push-in air pipe group and air inlet linkage assembly can be quickly inserted and matched to realize bidirectional conduction; at the same time, after the separation of each component of the expansion equipment, the push-in air pipe group and air inlet linkage assembly can be quickly blocked, so as to ensure the on-off control of the pipe opening after the strain storage cabinet assembly 1 is disassembled and separated; when the strain storage cabinet assembly 1 is combined to form the expansion equipment, the lateral air supply assembly on both sides of each strain storage cabinet assembly 1 can be used to supply hot air and materials of corresponding temperature, and at the same time, the push-in air pipe group and air inlet linkage assembly are connected with each other to realize the connection and flow guiding of each culture bin 301 inside in up-down stacking mode, ensure the connectivity of the airflow in each culture bin 301, and improve the effective control of the internal environment.

[0052] The above embodiments are only used to illustrate the technical solutions of the present application, but not limited thereto; although the present application has been described in detail with reference to the above embodiments, those skilled in the art should understand that the technical solutions recorded in the above embodiments can still be modified, or some or all of the technical features can be replaced by equivalents; and these modifications or replacements do not make the essence of the corresponding technical solutions deviate from the scope of the technical solutions of the embodiments of the present application, and they should be covered in the scope of the claims and the specification of the present application; any alternative improvement or change made by those skilled in the art to the embodiments of the present application falls within the protection scope of the present application.

[0053] The details of the present application not described are well known to those skilled in the art.

Claims

1. A microbial strain propagation and cultivation device, characterized in that: The system includes several mushroom spawn storage cabinet components stacked from top to bottom. Each mushroom spawn storage cabinet component has several supporting columns installed at its bottom. Each mushroom spawn storage cabinet component has several insertion holes installed at its top that mate with the supporting columns on the lower mushroom spawn storage cabinet components. Each mushroom spawn storage cabinet component has a push-in air intake pipe assembly at its top center and an air intake linkage assembly at its bottom center. The microbial storage cabinet assembly includes a horizontal microbial cabinet, and several layers of drawer-type culture trays are arranged inside the culture chamber of the horizontal microbial cabinet. The matching push-in air pipe assembly and air intake linkage component are plugged in to achieve bidirectional flow, thereby enabling the connection and airflow inside each of the stacked culture chambers; The push-in air pipe assembly includes a push-in pipe fixedly installed at the top center of the horizontal microbial community cabinet. The interior of the push-in pipe is connected to the interior of the corresponding culture chamber of the horizontal microbial community cabinet. A first fixing ring and a second fixing ring are fixedly installed from top to bottom inside the cavity of the push-in pipe. A push-in one-way rubber valve ball is installed in the cavity between the first fixing ring and the second fixing ring. The top of the push-in one-way rubber valve ball seals against the inner ring of the first fixing ring. A first spring is fixed at the bottom of the push-in one-way rubber valve ball. The bottom of the first spring is fixedly connected to the top of the second fixing ring. A lower vertical push-in column is fixed at the top center of the push-in one-way rubber valve ball. The top of the lower vertical push-in column extends to the upper part of the push-in pipe. The outer diameter of the lower vertical push-in column is smaller than the inner diameter of the inner ring of the first fixing ring. The air intake linkage assembly includes a sealing sleeve pressure pipe fixedly installed at the bottom center of the horizontal microbial community cabinet. The interior of the sealing sleeve pressure pipe is connected to the interior of the corresponding culture chamber of the horizontal microbial community cabinet. A third fixing ring and a fourth fixing ring are fixedly installed sequentially from top to bottom inside the cavity of the sealing sleeve pressure pipe. A passive one-way rubber valve ball is provided in the cavity between the third fixing ring and the fourth fixing ring. The bottom of the passive one-way rubber valve ball seals against the inner ring of the fourth fixing ring. A second spring is fixedly connected to the top of the passive one-way rubber valve ball. The top of the second spring abuts against the bottom of the third fixing ring. The inner ring of the fourth fixing ring is coaxially arranged with the lower vertical push column, and its inner diameter matches the outer diameter of the lower vertical push column.

2. The microbial strain propagation equipment according to claim 1, characterized in that: Lateral ventilation components are provided on both sides of each of the aforementioned microbial storage cabinet components.

3. The microbial strain propagation equipment according to claim 2, characterized in that: A double door is installed on the horizontal microbial culture cabinet at the front of the culture chamber, which is used to seal the inside of the culture chamber.

4. The microbial strain propagation equipment according to claim 3, characterized in that: The lateral ventilation assembly includes several lateral ventilation pipes with control valves, each of which is used to introduce gas into the culture chamber of the corresponding horizontal microbial community cabinet or to exhaust gas to the outside.

5. The microbial strain propagation equipment according to claim 4, characterized in that: The side wall of the horizontal microbial culture cabinet is also equipped with thermometers, hygrometers, and pressure gauges for detecting the temperature, humidity, and pressure inside the culture chamber.

6. The microbial strain propagation equipment according to claim 5, characterized in that: Each of the double doors is equipped with a transparent glass observation window for observing the interior of the corresponding culture chamber.

Images