A multi-channel microfluidic chip and a sample detection method

Abstract

The application relates to the technical field of immune detection chips, and provides a multi-channel micro-fluidic chip, which comprises a main body, a switching piece and a suction piece, the main body is provided with an inlet, at least two storage channels and at least two reaction channels, the switching piece is rotatably arranged on the main body and is provided with a suction cavity at one end, the suction cavity is communicated with each of the reaction channels, and the suction piece is slidably arranged in the suction cavity; wherein the switching piece has at least a first state and a second state and is switched by rotation; when the switching piece is in the first state, the switching piece sequentially connects the inlet, the at least two storage channels and the suction cavity; when the switching piece is in the second state, the switching piece sequentially connects one of the storage channels, the reaction channel corresponding to the storage channel and the suction cavity. Based on this, the detection efficiency can be improved. In addition, a sample detection method is also provided.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of immune detection chips, in particular to a multi-channel microfluidic chip and a sample detection method. BACKGROUND

[0002] Microfluidic technology can integrate sample preparation, reaction, separation and other basic operation units in biochemical analysis process on a chip to automatically complete the analysis process, which has the advantages of less sample consumption, fast detection speed and simple operation, and is often used in marker detection in the medical field to realize the diagnosis of related diseases.

[0003] At present, the existing microfluidic immune detection chip includes a main body and a suction member, the main body is provided with an inlet, a reaction channel and a suction cavity in sequence in communication, and the suction member is slidably arranged in the suction cavity. The detection instrument pulls the suction member to form negative pressure in the suction cavity, the sample flows from the inlet to the reaction channel for reaction, and then the detection instrument scans the reaction area to realize the detection of the marker.

[0004] For the related technology in the above, the existing microfluidic immune detection chip can only detect one index, and the detection efficiency is low, so it needs to be improved. SUMMARY

[0005] In order to improve the detection efficiency, in the first aspect, the present application provides a multi-channel microfluidic chip and.

[0006] The multi-channel microfluidic chip provided by the present application adopts the following technical scheme:

[0007] A multi-channel microfluidic chip, comprising a main body, a switching member and a suction member, the main body is provided with an inlet, at least two storage channels and at least two reaction channels, the switching member is rotatably arranged in the main body and one end is provided with a suction cavity, the suction cavity is in communication with each of the reaction channels, and the suction member is slidably arranged in the suction cavity; wherein the switching member has at least a first state and a second state and is switched by rotation; when the switching member is in the first state, the switching member makes the inlet, at least two storage channels and the suction cavity in sequence in communication; when the switching member is in the second state, the switching member makes one of the storage channels, the reaction channel corresponding to the storage channel and the suction cavity in sequence in communication.

[0008] By adopting the technical scheme, during work, the sample to be detected is placed at the inlet, the rotating switching member is switched to the first state by the detection instrument, the detection instrument pulls the suction member, the storage channels and the reaction channels are in a negative pressure state, the sample to be detected flows into the storage channels under the driving of the negative pressure and is stored; then, the rotating switching member is switched to the second state by the detection instrument, the detection instrument pulls the suction member again, the sample in the storage channels flows to the corresponding reaction channels to be mixed and reacted, so that multiple indexes are detected simultaneously, and the detection efficiency is improved.

[0009] Preferably, the switching member is provided with a first connecting groove and at least two second connecting grooves, the first connecting groove and the at least two second connecting grooves are arranged along the length direction of the switching member; when the switching member is in the first state, the first connecting groove communicates the inlet with one of the storage channels, and the at least two second connecting grooves communicate the storage channels and the suction cavity.

[0010] By adopting the technical scheme, the first connecting groove and the second connecting groove are arranged, so that when the switching member is in the first state, the inlet, the at least two storage channels and the suction cavity are communicated, the structure is simple and convenient for integrated injection molding production.

[0011] Preferably, the switching member is provided with a third connecting groove and a fourth connecting groove, the third connecting groove and the fourth connecting groove are arranged along the length direction of the switching member; when the switching member is in the second state, the third connecting groove communicates the outside with one of the storage channels, and the fourth connecting groove communicates the storage channels and the corresponding reaction channels.

[0012] By adopting the technical scheme, the third connecting groove and the fourth connecting groove are arranged, so that when the switching member is in the second state, the outside, one of the storage channels, the corresponding reaction channels and the suction cavity are communicated, the structure is simple and convenient for integrated injection molding production.

[0013] Preferably, the main body is provided with a connecting channel, when the switching member is in the first state, the connecting channel is communicated with the suction cavity and the storage channel far away from the inlet respectively, and a first sensor is arranged at the connecting channel, the first sensor is used to detect whether the at least two storage channels are full of liquid.

[0014] By adopting the technical scheme, the connecting channel is arranged to communicate the suction cavity and the storage channel far away from the inlet, and the first sensor is arranged to accurately control the liquid in the storage channel, so that the detection accuracy of the sample is improved.

[0015] Preferably, the reaction channel comprises a dissolving area, a reaction area and a detection area, a second sensor is arranged between the reaction area and the detection area, and the second sensor is used to detect whether the sample after reaction enters the detection area completely.

[0016] By adopting the technical scheme, the second sensor is arranged to ensure that the sample after reaction enters the detection area completely, thereby improving the detection accuracy of the sample.

[0017] Preferably, the reaction area comprises at least one curved section and at least two straight sections, and the at least one curved section is connected to the at least two straight sections respectively.

[0018] By adopting the technical scheme, the curved section and the straight section are arranged to increase the flow distance of the sample, thereby making the sample mixing and reaction more sufficient.

[0019] Preferably, the main body is provided with a cleaning liquid cavity, and the switching piece has a fifth state; when the switching piece is in the fifth state, the switching piece makes the cleaning liquid cavity, the at least two storage channels and the suction cavity communicate in sequence.

[0020] By adopting the technical scheme, the cleaning liquid cavity is arranged, and when the switching piece is in the fifth state, the cleaning liquid passes through the cleaning liquid cavity, the at least two storage channels and the suction cavity in sequence to clean the storage channels and the reaction channel.

[0021] Preferably, the main body is provided with a waste liquid pool, and the waste liquid pool is communicated with the at least two reaction channels and the suction cavity respectively.

[0022] By adopting the technical scheme, the waste liquid pool is arranged to facilitate the collection of the waste liquid after detection, so as to avoid the pollution of the environment and the detection instrument as much as possible.

[0023] Preferably, the switching piece is provided with a rotating groove at an end away from the suction piece.

[0024] By adopting the technical scheme, the rotating groove is arranged to facilitate the rotation of the switching piece by the detection instrument.

[0025] In a second aspect, the application provides a sample detection method.

[0026] The sample detection method provided by the application adopts the following technical scheme:

[0027] A sample detection method, characterized in that the detection method is realized based on the chip as claimed in any one of the above, and specifically comprises the following steps:

[0028] S1, the sample to be detected is placed in the inlet, the detection instrument rotates the switching piece to make it in the first state, and the detection instrument pulls the suction piece to make each of the storage channels and the reaction channels in a negative pressure state, and the sample to be detected flows into each of the storage channels under the driving of the negative pressure;

[0029] S2, the detection instrument rotates the switching piece to make it in the second state, and the detection instrument again pulls the suction piece to make the storage channel and the corresponding reaction channel in a negative pressure state, wherein the sample to be detected in the storage channel flows to the corresponding reaction channel under the driving of the negative pressure to mix and react.

[0030] In summary, the present application includes at least one of the following beneficial technical effects:

[0031] 1. When working, the sample to be detected is placed in the inlet, the detection instrument rotates the switching piece to make it in the first state, and the detection instrument pulls the suction piece to make each of the storage channels and the reaction channels in a negative pressure state, and the sample to be detected flows into each of the storage channels under the driving of the negative pressure; then, the detection instrument rotates the switching piece to make it in the second state, and the detection instrument again pulls the suction piece to make the sample in each of the storage channels flow to the corresponding reaction channel to mix and react, thereby realizing simultaneous detection of multiple indexes and improving the detection efficiency;

[0032] 2. The main body, the switching piece and the suction piece are produced by integral injection molding, the immunodetection chip has a simple structure and high detection result accuracy, and is suitable for popularization and use;

[0033] 3. After detection is completed, cleaning is performed to avoid environmental pollution of waste liquid and pollution of the detection instrument as much as possible. BRIEF DESCRIPTION OF DRAWINGS

[0034] Figure 1 is a schematic diagram of the overall structure of the multi-channel microfluidic chip in the embodiment of the present application;

[0035] Figure 2 is a schematic diagram of the overall structure of the multi-channel microfluidic chip in the embodiment of the present application; Figure 1

[0036] Figure 3 is an exploded structural schematic diagram of the multi-channel microfluidic chip in the first state in the embodiment of the present application;

[0037] Figure 4 is a connection diagram of the main body and the switching piece in the embodiment of the present application;

[0038] Figure 5 is an exploded structural schematic diagram of the multi-channel microfluidic chip in the second state in the embodiment of the present application.

[0039] ​Label: 1, main body; 11, inlet; 12, storage channel; 13, reaction channel; 131, dissolution area; 132, reaction area; 132a, curved section; 132b, straight section; 133, detection area; 14, connecting channel; 141, first connecting section; 142, second connecting section; 15, rotating groove; 16, limiting groove; 17, cleaning liquid cavity; 18, waste liquid pool; 2, switching piece; 21, rotating groove; 22, limiting ring; 23, suction cavity; 24, through groove; 25, first connecting groove; 26, second connecting groove; 27, third connecting groove; 28, fourth connecting groove; 3, suction piece; 4, first sensor; 5, second sensor. DETAILED DESCRIPTION

[0040] The following will be described in detail in combination with the accompanying drawings. Figures 1-5 The present application is further described in detail.

[0041] The embodiment of the present application discloses a multi-channel microfluidic chip.

[0042] Referring to Figure 1 and Figure 2 , the multi-channel microfluidic immune detection chip comprises a main body 1, a switching piece 2 and a suction piece 3, the main body 1 is provided with an inlet 11, at least two storage channels 12 and at least two reaction channels 13, in the embodiment, the number of the storage channels 12 and the reaction channels 13 is three, and the three storage channels 12 and the three reaction channels 13 are correspondingly and staggeredly arranged. At the same time, the main body 1 is also provided with a connecting channel 14 and a rotating groove 15, the rotating groove 15 is respectively communicated with the inlet 11, the three storage channels 12, the three reaction channels 13 and the connecting channel 14, that is, the interfaces of the inlet 11, the storage channels 12, the reaction channels 13 and the connecting channel 14 are respectively communicated with the rotating groove 15, so that the conduction of each channel is realized by adjusting the state of the switching piece 2.

[0043] Referring to Figure 2 and Figure 3 , the switching piece 2 is in a cylindrical shape, and the switching piece 2 is rotatably arranged in the rotating groove 15, one end of the switching piece 2 is provided with a rotating groove 21, the rotating groove 21 is used for cooperating with the connection of a detection instrument, so that the rotation of the switching piece 2 is controlled by the detection instrument. It can be understood that, according to different adaptation requirements of the detection instrument, the switching piece 2 can also be adapted to the detection instrument in other forms, such as a clamping block, a magnetic piece and the like, and the embodiment of the present application is not limited.

[0044] The switching piece 2 is provided with a suction cavity 23 at one end away from the rotating groove 21, the suction cavity 23 is isolated from the rotating groove 21, and the switching piece 2 is provided with a through groove 24 along the circumferential side of the switching piece 2, which is used to connect the channel 14 with the suction cavity 23 at all times. The suction piece 3 is slidably arranged in the suction cavity 23, and a detection instrument is connected to the operating end of the suction piece 3. By pulling the suction piece 3, air in the connecting channel 14 is sucked into the suction cavity 23, so as to generate a negative pressure state.

[0045] The switching piece 2 is further provided with a limiting ring 22. In the embodiment, the limiting ring 22 is formed by protruding along the circumferential direction of the switching piece 22. The main body 1 is provided with a limiting groove 16 at the rotating groove 15, and the limiting ring 22 is limited in rotation in the limiting groove 16, so as to improve the stability of the rotation of the switching piece 2.

[0046] The switching piece 2 has a first state, a second state, a third state and a fourth state, and the states are switched by rotation.

[0047] When the switching piece 2 is in the first state, the switching piece 2 makes the inlet 11, the three storage channels 12, the connecting channel 14 and the suction cavity 23 sequentially communicate. When the switching piece 2 is in the second state, the third state and the fourth state, the switching piece 2 respectively makes one of the storage channels 12, the corresponding reaction channel 13, the connecting channel 14 and the suction cavity 23 sequentially communicate. In operation, the sample to be detected is placed in the inlet 11. The detection instrument is used to rotate the switching piece 2 to make it in the first state, and the detection instrument is used to pull the suction piece 3, so that each storage channel 12 and the reaction channel 13 are in a negative pressure state. The sample to be detected flows into the three storage channels 12 under the driving of the negative pressure and is stored. Then, the detection instrument is used to rotate the switching piece 2 to make it in the second state, so that the sample in one of the storage channels 12 flows to the corresponding reaction channel 13 for mixing and reaction. Then, the switching piece 2 is rotated to the third state, so that the sample in another storage channel 12 flows to the corresponding reaction channel 13 for mixing and reaction. Finally, the switching piece 2 is switched to the fourth state, so that the sample in the remaining storage channel 12 flows to the corresponding reaction channel 13 for mixing and reaction. In this way, multiple indexes can be detected at the same time, and the detection efficiency is improved.

[0048] Reference Figure 3 and Figure 4Specifically, the switching piece 2 is provided with a first connecting groove 25 and three second connecting grooves 26 on the circumferential side of the switching piece 2, and the first connecting groove 25 and the three second connecting grooves 26 are arranged at intervals along the length direction of the switching piece 2. When the switching piece 2 is in the first state, the first connecting groove 25 makes the inlet 11 communicate with the storage channel 12 close to the inlet 11, two of the second connecting grooves 26 make the three storage channels 12 communicate, and the other second connecting groove 26 makes the storage channel 12 far away from the inlet 11 communicate with the connecting channel 14, so as to realize the sequential communication of the inlet 11, the three storage channels 12, the connecting channel 14 and the suction cavity 23, and the structure is simple and convenient for integrated injection molding production.

[0049] With reference to Figure 4 and Figure 5 Specifically, the switching piece 2 is provided with a third connecting groove 27 and a fourth connecting groove 28 on the circumferential side of the switching piece 2, and the third connecting groove 27 and the fourth connecting groove 28 are arranged at intervals along the length direction of the switching piece 2. When the switching piece 2 is in the second state, one end of the third connecting groove 27 extends to the end of the switching piece 2 and communicates with the external environment, and the other end communicates with one of the storage channels 12, and the fourth connecting groove 28 makes the storage channel 12 communicate with the corresponding reaction channel 13, so as to realize the sequential communication of the external environment, the storage channel 12, the corresponding reaction channel 13, the connecting channel 14 and the suction cavity 23. Due to the communication with the external environment, when the suction piece 3 is pulled again, the sample in the storage channel 12 can flow to the corresponding reaction channel 13.

[0050] It should be noted that in the second state, the third state and the fourth state, one of the storage channels 12 and the corresponding reaction channel 13 can be realized, respectively, and correspondingly, the number of the third connecting grooves 27 and the fourth connecting grooves 28 on the switching piece 2 is three groups, and each group is arranged at intervals along the circumferential direction of the switching piece 2, and the size and position of the third connecting grooves 27 and the fourth connecting grooves 28 in different groups are different, so as to realize the conduction of different storage channels 12 and the corresponding reaction channels 13.

[0051] When the switching piece 2 is in the third state or the fourth state, the situation is the same as when the switching piece 2 is in the second state, respectively making the other two storage channels 12 and the corresponding reaction channels 13 communicate, which will not be described one by one here.

[0052] Further, the main body 1 is further provided with a cleaning liquid cavity 17 and a waste liquid pool 18, the cleaning liquid cavity 17 is placed with a bubble cap (not shown in the figure), the cleaning liquid cavity 17 is communicated with the inlet 11, and the waste liquid pool 18 is communicated with the three reaction channels 13 and the connecting channel 14 respectively. Moreover, the switching piece 2 has a fifth state, when the switching piece 2 is in the fifth state, the switching piece 2 makes the cleaning liquid cavity 17, the three storage channels 12, the connecting channel 14 and the suction cavity 23 communicate in sequence, and the specific communication structure is the same as when the switching piece 2 is in the first state. When working, the detection instrument blocks the inlet 11 and squeezes the bubble cap, and the cleaning liquid in the bubble cap enters the three storage channels 12 through the cleaning liquid cavity 17.

[0053] Wherein, the connecting channel 14 includes a first connecting section 141 and a second connecting section 142, one end of the first connecting section 141 and the second connecting section 142 is communicated with the waste liquid pool 18, the other end of the second connecting section 142 is communicated with the suction cavity 23, and is separated from the first connecting section 141 142, in this way, the sample or the cleaning liquid can be avoided entering the suction cavity 23 as far as possible.

[0054] The first connecting section 141 is provided with a first sensor 4, the first sensor 4 is used for detecting whether the three storage channels 12 are full of liquid, so as to accurately control the liquid in the storage channel 12, and the detection accuracy of the sample is improved. It should be noted that the first sensor 4 is electrically connected with the detection instrument, so that the detection instrument controls the suction of the suction piece 3 and the switching according to the signal of the first sensor 4.

[0055] Specifically, the reaction channel 13 includes a dissolution region 131, a reaction region 132 and a detection region 133 in sequence, the dissolution region 131 is provided with a freeze-dried reagent, the sample dissolves the freeze-dried reagent when passing through the dissolution region 131, then mixes and reacts in the reaction region 132, and then enters the detection region 133 for detection, a second sensor 5 is arranged between the reaction region 132 and the detection region 133, the second sensor 5 is used for detecting whether the sample after reaction enters the detection region 133 completely, so as to ensure that the sample after reaction enters the detection region 133 completely, thereby improving the detection accuracy of the sample. It should be noted that the second sensor 5 is electrically connected with the detection instrument, so that the detection instrument controls the suction of the suction piece 3 and the switching according to the signal of the second sensor 5.

[0056] Wherein, the reaction region 132 includes at least one curved section 132a and at least two straight sections 132b, the two ends of the at least one curved section 132a are connected with the at least two straight sections 132b respectively, so that the reaction region 132 is in a snake shape, thereby increasing the flow distance of the sample, and further making the sample mix and react more fully.

[0057] The implementation principle of the embodiment is that: in operation, the sample to be detected is placed at the inlet 11, the detection instrument rotates the switching piece 2 to make it in the first state, and the detection instrument pulls the suction piece 3 to make each storage channel 12 and the reaction channel 13 in a negative pressure state, the sample to be detected flows into the three storage channels 12 under the driving of the negative pressure and is stored; then the detection instrument rotates the switching piece 2 to make it in the second state, so that the sample in one storage channel 12 flows to the corresponding reaction channel 13 for mixing and reaction, then the switching piece 2 is rotated to the third state, so that the sample in another storage channel 12 flows to the corresponding reaction channel 13 for mixing and reaction, and finally the switching piece 2 is switched to the fourth state, so that the sample in the remaining storage channel 12 flows to the corresponding reaction channel 13 for mixing and reaction. In this way, multiple indexes can be detected at the same time, and the detection efficiency is improved.

[0058] The application also discloses a sample detection method, which is realized based on the chip provided in the above embodiment. The detection method comprises the following steps:

[0059] S1, the sample to be detected is placed at the inlet 11, the detection instrument rotates the switching piece 2 to make it in the first state, and the detection instrument pulls the suction piece 3 to make each storage channel 12 and the reaction channel 13 in a negative pressure state, the sample to be detected flows into the three storage channels 12 and the connecting channel 14 under the driving of the negative pressure, and the first sensor 4 detects that the three storage channels 12 are full of the sample to be detected, and the detection instrument stops pulling the suction piece 3;

[0060] S2, the detection instrument rotates the switching piece 2 to make it in the second state, and the detection instrument pulls the suction piece 3 to make one storage channel 12 and the corresponding reaction channel 13 in a negative pressure state, and the sample flows from the storage channel 12 to the corresponding reaction channel 13 under the driving of the negative pressure;

[0061] S3, the sample to be detected first enters the dissolving area 131, dissolves the freeze-dried reagent in the area, then enters the reaction area 132 for mixing and reaction, and then enters the detection area 133, and the second sensor 5 detects that the sample to be detected after reaction all flows into the detection area 133, and the detection instrument stops pulling the suction piece 3, the antigen-antibody is fully combined here, and the incubation timing starts;

[0062] S4, the detection instrument rotates the switching piece 2 to make it in the third state, and the operation process is the same as that when the detection instrument rotates the switching piece 2 to make it in the second state;

[0063] S5, the detection instrument rotates the switching piece 2 to make it in the fourth state, and the operation process is the same as that when the detection instrument rotates the switching piece 2 to make it in the second state;

[0064] S6, after the incubation time of each detection area 133, repeat S2, S4 and S5 to make the residual liquid of each detection area 133 flow into the waste pool 18;

[0065] S7, the detection instrument rotates the switching member 2 to the fifth state, the detection instrument blocks the sample inlet 11, and the detection instrument squeezes the bubble cap to make the cleaning liquid into the cleaning liquid chamber 17, the cleaning liquid flows along the three storage channels 12, at the same time, the detection instrument pulls the suction member 3, when the first sensor 4 detects that the three storage channels 12 are full of cleaning liquid, the detection instrument stops pulling the suction member 3;

[0066] S8, repeat S2, S4 and S5 to make the cleaning liquid flow through each reaction channel 13 and then enter the waste pool 18;

[0067] S9, the detection instrument scans the fluorescence signal of each detection area 133 to obtain the result.

[0068] The above are the preferred embodiments of the present application, which do not limit the protection scope of the present application, therefore: any equivalent changes made on the structure, shape and principle of the present application should be covered within the protection scope of the present application.

Claims

1. A multi-channel microfluidic chip, characterized in that, The device includes a main body (1), a switching component (2), and a suction component (3). The main body (1) is provided with an inlet (11), at least two storage channels (12), and at least two reaction channels (13). The switching component (2) is rotatably disposed on the main body (1) and has a suction chamber (23) at one end. The suction chamber (23) is connected to each of the reaction channels (13). The suction component (3) is slidably disposed on the suction chamber (23). The switching element (2) has at least a first state and a second state and can be switched by rotation; when the switching element (2) is in the first state, the switching element (2) connects the inlet (11), at least two of the storage channels (12), and the suction chamber (23) in sequence; when the switching element (2) is in the second state, the switching element (2) connects one of the storage channels (12), the reaction channel (13) corresponding to the storage channel (12), and the suction chamber (23) in sequence. The switching component (2) is provided with a first connecting groove (25) and at least two second connecting grooves (26) on its periphery. The first connecting groove (25) and the at least two second connecting grooves (26) are spaced apart along the length direction of the switching component (2). When the switching component (2) is in the first state, the first connecting groove (25) connects the inlet (11) to one of its storage channels (12), and the at least two second connecting grooves (26) connect each of the storage channels (12) and the suction chamber (23). The switching component (2) is provided with a third connecting groove (27) and a fourth connecting groove (28) on its periphery. The third connecting groove (27) and the fourth connecting groove (28) are spaced apart along the length direction of the switching component (2). When the switching component (2) is in the second state, the third connecting groove (27) connects the outside to one of its storage channels (12), and the fourth connecting groove (28) connects the storage channel (12) to the corresponding reaction channel (13).

2. The multi-channel microfluidic chip according to claim 1, characterized in that, The main body (1) is provided with a connecting channel (14). When the switching component (2) is in the first state, the connecting channel (14) is connected to the suction chamber (23) and the storage channel (12) away from the inlet (11) respectively. A first sensor (4) is provided at the connecting channel (14). The first sensor (4) is used to detect whether at least two of the storage channels (12) are full of liquid.

3. A multi-channel microfluidic chip according to claim 1, characterized in that, The reaction channel (13) includes a dissolution region (131), a reaction region (132), and a detection region (133). A second sensor (5) is provided between the reaction region (132) and the detection region (133). The second sensor (5) is used to detect whether the sample after the reaction has completely entered the detection region (133).

4. A multi-channel microfluidic chip according to claim 3, characterized in that, The reaction region (132) includes at least one curved segment (132a) and at least two straight segments (132b), wherein at least one curved segment (132a) is connected to at least two straight segments (132b).

5. A multi-channel microfluidic chip according to claim 1, characterized in that, The main body (1) is provided with a cleaning fluid chamber (17), and the switching member (2) has a fifth state; when the switching member (2) is in the fifth state, the switching member (2) connects the cleaning fluid chamber (17), at least two of the storage channels (12), and the suction chamber (23) in sequence.

6. A multi-channel microfluidic chip according to claim 5, characterized in that, The main body (1) is provided with a waste liquid tank (18), which is connected to at least two of the reaction channels (13) and the suction chamber (23).

7. A multi-channel microfluidic chip according to claim 1, characterized in that, The switching component (2) has a rotating groove (21) at the end away from the suction component (3).

8. A sample detection method, characterized in that, The detection method is implemented based on the chip described in any one of claims 1-7, and specifically includes the following steps: S1. The sample to be tested is placed in the inlet (11), the testing instrument rotates the switching component (2) to make it in the first state, and the testing instrument pulls the suction component (3) to make each of the storage channels (12) and the reaction channel (13) in a negative pressure state, and the sample to be tested flows into each of the storage channels (12) under the drive of the negative pressure. S2. The detection instrument rotates the switching component (2) to put it in the second state, and the detection instrument pulls the suction component (3) again to put one of its storage channels (12) and the corresponding reaction channel (13) into a negative pressure state, wherein the sample to be tested in one of the storage channels (12) flows to the corresponding reaction channel (13) under the drive of the negative pressure to carry out the reaction.

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