A pesticidal composition containing ethoprophos and its application

Abstract

The present invention belongs to the technical field of pesticides, and particularly relates to a pesticidal composition of ethoprophos, comprising components A and B, wherein component A is one or a combination of trans-aconitic acid, jasmonic acid, and thymol; and component B is ethoprophos. The compound nematicidal composition can significantly improve the nematicidal activity, and can be processed into common dosage forms applied in the agricultural field such as emulsifiable concentrates, granules, emulsions in water, aqueous solutions, etc., for controlling nematode diseases of vegetable crops, food crops, and fruit trees. It has a low dosage, outstanding effects, can delay the resistance problems existing in existing chemical agents, and meets the safety requirements of pesticide formulations.

Description

Technical Field

[0001] The present invention relates to the technical field of pesticides, and particularly relates to a pesticide composition containing ethoprophos-methyl and its application. Background Art

[0002] Root-knot nematode disease is one of the important crop diseases caused by nematodes of the genus Meloidogyne spp. Root-knot nematodes can infect more than 3000 plant species, including vegetables, grains, cash crops, fruit trees and ornamental plants. Crops infected with root-knot nematodes will have a reduction in yield of 10% - 20% at a minimum, and more than 75% at a maximum. The economic losses caused globally every year reach billions of US dollars. In China, with the increase in the planting area of protected vegetables, especially since the large-scale popularization of solar greenhouses, the multiple cropping index has increased. Coupled with serious continuous cropping, it has provided a suitable environment for the occurrence and development of root-knot nematode disease, enabling the accumulation and proliferation of root-knot nematodes in the soil and becoming an important disease endangering crop production in China. Currently, the control of root-knot nematodes mainly relies on chemical pesticides. However, with the increasing prominence of environmental problems and pesticide residues on crops, reducing the use of chemical pesticides has become a trend.

[0003] Using plants, microorganisms or their metabolites to control vegetable root-knot nematodes can reduce pesticide residues and is conducive to the sustainable development of agricultural production. Patent CN103416400A discloses the use of trans-aconitic acid (CAS: 4023-65-8) in controlling plant root-knot nematodes, with a usage concentration of 100 - 1000 μg / mL for Meloidogyne incognita, and the optimal usage concentration being 500 μg / mL; Li Xiaoman et al. (2017, Journal of Beijing University of Agriculture), Liu Qing'an et al. (2008, Journal of Nanjing Agricultural University) and others have studied the effect of jasmonic acid on root-knot nematodes. The experiments show that jasmonic acid can affect the development of nematodes. Summary of the Invention

[0004] To solve the problem of difficult nematode control, the present invention provides a pesticide composition containing ethoprophos-methyl, which solves the problem of the resistance risk of using chemical pesticides alone. At the same time, it can significantly reduce the dosage of pesticides and also has an obvious improvement effect on crop growth and fruit quality.

[0005] The present invention provides a pesticide composition, which comprises component A and component B (active ingredients);

[0006] Among them, component A is selected from any one, two or more of trans-aconitic acid, jasmonic acid, and thymol; component B is ethoprophos-methyl.

[0007] In some technical solutions, the mass ratio of component A to component B is 80 to 1:1 to 80, preferably 20 to 1:1 to 20, more preferably 20 to 10:5 to 10, such as 20:10, 10:5.

[0008] In some technical solutions, the weight percentage content of component A and component B in the pesticide composition is 1-95%, preferably 10-75%, more preferably 15-50%, such as 30%, 15%.

[0009] In some technical solutions, the pesticide composition further comprises agriculturally acceptable adjuvants and / or carriers.

[0010] In some technical solutions, the adjuvants and / or carriers include fillers, surfactants, etc., such as one, two or more of dispersants, wetting agents, emulsifiers, solvents, thickeners, antifreezes, binders, disintegrants, foaming agents, defoaming agents, penetrants, stabilizers, carriers.

[0011] In some technical solutions, the dosage form of the pesticide composition is selected from liquid dosage forms, solid dosage forms, such as soluble solutions, emulsifiable concentrates, emulsions, aqueous solutions, granules, etc. Among them, the liquid dosage form is used for drip irrigation and flushing, and the solid dosage form is used for broadcasting.

[0012] In some technical solutions, the dosage form of the pesticide composition is a granule; preferably, the granule comprises the following components: component B, component A, alkylnaphthalenesulfonate, fatty alcohol polyoxyethylene ether sulfate, silica white, polyvinyl alcohol, ammonium sulfate, magnesium stearate, kaolin.

[0013] In some technical solutions, the granule comprises the following components: component B, trans-aconitic acid, alkylnaphthalenesulfonate, fatty alcohol polyoxyethylene ether sulfate, silica white, polyvinyl alcohol, ammonium sulfate, magnesium stearate, kaolin; preferably, the granule comprises the following components: 5 parts of component B, 10 parts of trans-aconitic acid, 3 parts of alkylnaphthalenesulfonate, 2 parts of fatty alcohol polyoxyethylene ether sulfate, 5 parts of silica white, 2 parts of polyvinyl alcohol, 5 parts of ammonium sulfate, 5 parts of magnesium stearate, 63 parts of kaolin.

[0014] In some technical solutions, the granule comprises the following components: component B, jasmonic acid, alkylnaphthalenesulfonate, fatty alcohol polyoxyethylene ether sulfate, silica white, polyvinyl alcohol, ammonium sulfate, magnesium stearate, kaolin; preferably, the granule comprises the following components: 5 parts of component B, 10 parts of jasmonic acid, 3 parts of alkylnaphthalenesulfonate, 2 parts of fatty alcohol polyoxyethylene ether sulfate, 5 parts of silica white, 2 parts of polyvinyl alcohol, 5 parts of ammonium sulfate, 5 parts of magnesium stearate, 63 parts of kaolin.

[0015] In some technical solutions, the granule comprises the following components: Component B, thymol, alkylnaphthalenesulfonate, fatty alcohol polyoxyethylene ether sulfate, silica white, polyvinyl alcohol, ammonium sulfate, magnesium stearate, kaolin; preferably, the granule comprises the following components: 5 parts of Component B, 10 parts of thymol, 3 parts of alkylnaphthalenesulfonate, 2 parts of fatty alcohol polyoxyethylene ether sulfate, 5 parts of silica white, 2 parts of polyvinyl alcohol, 5 parts of ammonium sulfate, 5 parts of magnesium stearate, 63 parts of kaolin.

[0016] In some technical solutions, the dosage form of the pesticide composition is a soluble concentrate; preferably, the soluble concentrate comprises the following components: Component B, Component A, secondary alcohol polyoxyethylene ether, polyoxyethylene alkylamine, propylene glycol, N-methylpyrrolidone.

[0017] In some technical solutions, the soluble concentrate comprises the following components: Component B, trans-aconitic acid, secondary alcohol polyoxyethylene ether, polyoxyethylene alkylamine, propylene glycol, N-methylpyrrolidone; preferably, the soluble concentrate comprises the following components: 10 parts of Component B, 20 parts of trans-aconitic acid, 8 parts of secondary alcohol polyoxyethylene ether, 1 part of polyoxyethylene alkylamine, 20 parts of propylene glycol, 41 parts of N-methylpyrrolidone.

[0018] In some technical solutions, the soluble concentrate comprises the following components: Component B, jasmonic acid, secondary alcohol polyoxyethylene ether, polyoxyethylene alkylamine, propylene glycol, N-methylpyrrolidone; preferably, the soluble concentrate comprises the following components: 10 parts of Component B, 20 parts of jasmonic acid, 8 parts of secondary alcohol polyoxyethylene ether, 1 part of polyoxyethylene alkylamine, 20 parts of propylene glycol, 41 parts of N-methylpyrrolidone.

[0019] In some technical solutions, the soluble concentrate comprises the following components: Component B, thymol, secondary alcohol polyoxyethylene ether, polyoxyethylene alkylamine, propylene glycol, N-methylpyrrolidone; preferably, the soluble concentrate comprises the following components: 10 parts of Component B, 20 parts of thymol, 8 parts of secondary alcohol polyoxyethylene ether, 1 part of polyoxyethylene alkylamine, 20 parts of propylene glycol, 41 parts of N-methylpyrrolidone.

[0020] In some technical solutions, the dosage form of the pesticide composition is an emulsifiable concentrate; preferably, the emulsifiable concentrate comprises the following components: Component B, Component A, fatty alcohol polyoxyethylene ether, triethanolamine salt of triphenylethylphenol polyoxyethylene ether phosphate, cyclohexanone, absolute ethanol, solvent oil.

[0021] In some technical solutions, the emulsifiable concentrate comprises the following components: Component B, jasmonic acid, fatty alcohol polyoxyethylene ether, triethanolamine salt of triphenylethylphenol polyoxyethylene ether phosphate, cyclohexanone, absolute ethanol, solvent oil; preferably, the emulsifiable concentrate comprises the following components: 10 parts of Component B, 20 parts of jasmonic acid, 6 parts of fatty alcohol polyoxyethylene ether, 2 parts of triethanolamine salt of triphenylethylphenol polyoxyethylene ether phosphate, 20 parts of cyclohexanone, 5 parts of absolute ethanol, and 37 parts of solvent oil.

[0022] In some technical solutions, the emulsifiable concentrate comprises the following components: Component B, trans-aconitic acid, fatty alcohol polyoxyethylene ether, triethanolamine salt of triphenylethylphenol polyoxyethylene ether phosphate, cyclohexanone, absolute ethanol, solvent oil; preferably, the emulsifiable concentrate comprises the following components: 10 parts of Component B, 20 parts of trans-aconitic acid, 6 parts of fatty alcohol polyoxyethylene ether, 2 parts of triethanolamine salt of triphenylethylphenol polyoxyethylene ether phosphate, 20 parts of cyclohexanone, 5 parts of absolute ethanol, and 37 parts of solvent oil.

[0023] In some technical solutions, the emulsifiable concentrate comprises the following components: Component B, thymol, fatty alcohol polyoxyethylene ether, triethanolamine salt of triphenylethylphenol polyoxyethylene ether phosphate, cyclohexanone, absolute ethanol, solvent oil; preferably, the emulsifiable concentrate comprises the following components: 10 parts of Component B, 20 parts of thymol, 6 parts of fatty alcohol polyoxyethylene ether, 2 parts of triethanolamine salt of triphenylethylphenol polyoxyethylene ether phosphate, 20 parts of cyclohexanone, 5 parts of absolute ethanol, and 37 parts of solvent oil.

[0024] The present invention also provides the use of the above pesticide composition, and the pesticide composition is used for preventing and controlling plant diseases and pests, preferably for preventing and controlling plant nematodes, such as root-knot nematodes, stem nematodes (such as Ditylenchus destructor), cyst nematodes, root rot nematodes, and Bursaphelenchus xylophilus.

[0025] In some technical solutions, the pesticide composition can be used for soil treatment during the seedling stage of plants or before transplanting seedlings.

[0026] In some technical solutions, the plants include vegetables, fruit trees, cash crops, garden trees, etc.; vegetables such as: tomatoes, cucumbers, loofahs, gourds, peas, bell peppers, chili peppers, lettuce, potatoes, celery, etc.; fruit trees such as: citrus, oranges, grapefruits, lemons, bananas, sugarcane, mangoes, pineapples, lychees, guavas, durians, etc.; cash crops such as: cotton, cocoa, coffee, peanuts, tobacco, corn, soybeans, etc.; garden trees such as: fir trees, pine trees, etc.

[0027] The present invention also provides a preparation method of the above pesticide composition, which is prepared by using conventional methods in the pesticide field; for example, by mixing the active ingredient with a filler (i.e., a liquid solvent and / or a solid carrier), and selecting a suitable surfactant (i.e., an emulsifier and / or a dispersant and / or a foaming agent and / or a penetrant and / or a stabilizer).

[0028] Beneficial effects:

[0029] The pesticide composition provided by the present invention, by combining ethoprophos with trans-aconitic acid, jasmonic acid or thymol, has a low dosage, outstanding effects, can delay the problem of drug resistance existing in existing chemical agents, and meets the safety requirements of pesticide formulations. The pesticide composition provided by the present invention has significantly improved the lethal effect on root-knot nematodes, significantly improved the growth of crops and the quality of fruits, enhanced the plant vigor, increased the crop yield, and delayed the generation of resistance. Specific embodiments

[0030] The technical solutions of the present invention will be further described in detail below in conjunction with specific embodiments. It should be understood that the following embodiments are only used to illustrate and explain the present invention exemplarily, and should not be construed as limiting the protection scope of the present invention. All technologies implemented based on the above content of the present invention are covered within the scope of protection intended by the present invention.

[0031] Unless otherwise specified, the raw materials and reagents used in the following examples are all commercially available products, or can be prepared by known methods.

[0032] Example 1

[0033] The nematicidal composition is a soluble concentrate composed of the following components: 10% ethoprophos, 20% trans-aconitic acid, 8% secondary alcohol polyoxyethylene ether, 1% polyoxyethylene alkylamine, 20% propylene glycol, and made up to 100% with N-methylpyrrolidone.

[0034] Example 2

[0035] The nematicidal composition is a soluble concentrate composed of the following components: 10% ethoprophos, 20% jasmonic acid, 8% secondary alcohol polyoxyethylene ether, 1% polyoxyethylene alkylamine, 20% propylene glycol, and made up to 100% with N-methylpyrrolidone.

[0036] Example 3

[0037] The nematicidal composition is a soluble concentrate composed of the following components: 10% ethoprophos, 20% thymol, 5% dioctyl phthalate, 5% polyoxyethylene sorbitan, 20% n-butanol, 10% N,N-dimethylformamide, and made up to 100% with dimethyl sulfoxide.

[0038] Example 4

[0039] The nematicidal composition is a granule composed of the following components: 5% ethoprophos, 10% jasmonic acid, 3% alkylnaphthalenesulfonate, 2% fatty alcohol polyoxyethylene sulfate, 5% white carbon black, 2% polyvinyl alcohol, 5% ammonium sulfate, 5% magnesium stearate, and made up to 100% with kaolin.

[0040] Example 5

[0041] The nematocide composition is a granule composed of the following components: 5% of ethoprophos methyl, 10% of trans-aconitic acid, 3% of alkyl naphthalene sulfonate, 2% of fatty alcohol polyoxyethylene ether sulfate, 5% of silica white, 2% of polyvinyl alcohol, 5% of ammonium sulfate, 5% of magnesium stearate, and the balance made up with kaolin.

[0042] Example 6

[0043] The nematocide composition is a granule composed of the following components: 5% of ethoprophos methyl, 10% of thymol, 3% of alkyl naphthalene sulfonate, 2% of fatty alcohol polyoxyethylene ether sulfate, 5% of silica white, 2% of polyvinyl alcohol, 5% of ammonium sulfate, 5% of magnesium stearate, and the balance made up with kaolin.

[0044] Example 7

[0045] The nematocide composition is an emulsifiable concentrate composed of the following components: 10% of ethoprophos methyl, 20% of jasmonic acid, 6% of fatty alcohol polyoxyethylene ether, 2% of triethanolamine salt of triphenylethylphenol polyoxyethylene ether phosphate, 20% of cyclohexanone, 5% of absolute ethanol, and the balance made up with solvent oil.

[0046] Example 8

[0047] The nematocide composition is an emulsifiable concentrate composed of the following components: 10% of ethoprophos methyl, 20% of trans-aconitic acid, 6% of fatty alcohol polyoxyethylene ether, 2% of triethanolamine salt of triphenylethylphenol polyoxyethylene ether phosphate, 20% of cyclohexanone, 5% of absolute ethanol, and the balance made up with solvent oil.

[0048] Example 9

[0049] The nematocide composition is an emulsifiable concentrate composed of the following components: 10% of ethoprophos methyl, 20% of thymol, 8% of fatty alcohol polyoxyethylene ether, 3% of triethanolamine salt of triphenylethylphenol polyoxyethylene ether phosphate, 20% of dimethyl sulfoxide, and the balance made up with solvent oil.

[0050] Test Example 1 Indoor Test

[0051] 1. Test Method

[0052] It was carried out with reference to the immersion method in Part 1: Test for inhibiting phytopathogenic nematodes of the Guidelines for Pesticide Bioassay in the Laboratory - Nematocides NY / T 1833.1 - 2009.

[0053] On the basis of determining the concentration range of the medicament in the preliminary test, the test medicament was diluted with an aqueous solution containing 0.1% Tween 80 into 5 - 7 series of concentrations, and the same dose of acetone was diluted with an aqueous solution containing 0.1% Tween 80 as a control. The collected nematodes were made into a suspension with clear water (about 200 nematodes / mL). 0.5 mL of the medicament solution and 0.5 mL of the nematode suspension were pipetted into a 2 mL counting dish, repeated 3 times. After mixing evenly with a fine glass rod, it was placed in a constant temperature incubator at 25°C. After 48 hours, the medicament solution in the counting dish was filtered out, and only the nematodes were retained. 1 mL of 2% sodium chloride solution was added and kept for 2 minutes. The nematodes with stiff bodies were dead worms, and the live worms curled or wriggled. The number of dead and live nematodes was checked, and the mortality rate and corrected mortality rate were calculated. Statistical analysis was carried out using DPS statistical analysis software. By calculating the LC 50 of each medicament to evaluate the nematicidal activity of each medicament, and the co-toxicity coefficient (CTC value) of the mixture was calculated according to the method of Sun Peiyun.

[0054] Mortality rate = number of dead nematodes / number of treated nematodes × 100%

[0055] Corrected mortality rate = (mortality rate of treatment - mortality rate of control) / (100 - mortality rate of control) × 100%

[0056] Actual toxicity index (ATI) = (LC 50 of standard medicament / LC 50 of test medicament) × 100

[0057] Theoretical toxicity index (TTI) = toxicity index of medicament A × percentage content of A in the mixture + toxicity index of medicament B × percentage content of B in the mixture + toxicity index of medicament C × percentage content of C in the mixture

[0058] Co-toxicity coefficient (CTC) = [actual toxicity index (ATI) of the mixture / theoretical toxicity index (TTI) of the mixture] × 100

[0059] According to the classification standard of joint action: co-toxicity coefficient (CTC) ≥ 120 indicates synergistic effect; co-toxicity coefficient (CTC) ≤ 80 indicates antagonistic effect; 80 < co-toxicity coefficient (CTC) < 120 indicates additive effect.

[0060] 2. Test results

[0061] Table 1 Test results of the inhibitory activity of the nematicidal compositions in Examples 1 - 3, 7 - 9 against the second-instar larvae of Meloidogyne

[0062]

[0063]

[0064] As can be seen from the data in Table 1, the nematode-killing composition of the present invention has a good control effect on the second-instar larvae of nematodes, and the two compounds can play a good synergistic effect.

[0065] Test Example 2 Field Efficacy Test

[0066] Test agents: Examples 1-9

[0067] Test crop: Cucumber

[0068] Control object: Root-knot nematodes

[0069] Dosage of active ingredient used: 1.5 kg / ha

[0070] Method for investigating disease index and control effect:

[0071] Crush the diseased roots of cucumber root knots, mix evenly with 9 times the weight of wet sandy soil to prepare a root-knot nematode inoculum. When planting cucumbers, apply it into the holes at a rate of about 180 kg·hm -2 for artificial inoculation. There are two application methods. One is to apply the granule to each hole individually before planting cucumbers. The other is to water and slow down the seedlings after planting. 7 days after planting, carry out medicament irrigation on the roots. No other nematode medicaments were applied during the test period.

[0072] One month after applying the medicine, investigate the plant height, stem diameter, chlorophyll content (measured with a chlorophyll meter), cucumber length, cucumber thickness, and cucumber sugar content (measured with a sugar meter) of each treatment of cucumbers, and then measure the yield for one month. Two months after applying the medicine, investigate the damage of root-knot nematodes. Grading method for the damage of root-knot nematode disease: Grade 0: Healthy seedlings without disease (no root knots on the roots); Grade 1: Root knots on less than 10% of the roots, but the root knots are not connected to each other; Grade 2: Root knots on 11%-30% of the roots, and only a small number of root knots are connected to each other; Grade 3: Root knots on 31%-50% of the roots, and less than half of the root knots are connected to each other; Grade 4: Root knots on 51%-75% of the roots, more than half of the root knots are connected to each other, and some main and lateral roots become thick and deformed; Grade 5: Root knots on more than 75% of the roots, and they are connected to each other, and most main and lateral roots become thick and deformed.

[0073]

[0074]

[0075] At the harvest stage, measure the single fruit weight, melon length, melon thickness, yield and sugar content of cucumbers.

[0076] Test results:

[0077] Table 2 Field control effect of the nematode-killing composition of Examples 1-9 on cucumber root-knot nematodes and plant growth

[0078]

[0079] Table 3 Field Yield and Fruit Quality of the Nematocide Compositions in Examples 1 - 9 against Meloidogyne incognita on Cucumber

[0080]

[0081] As can be seen from Table 2 and Table 3, after the compounding of ethoprophos with each medicament, the control effect on Meloidogyne incognita can be significantly improved, the plant height, stem diameter, chlorophyll content, etc. of cucumber plants can be increased, the cucumber yield can be increased, and the cucumber quality can be improved.

[0082] Test Example 3 Field Efficacy Test

[0083] Test Medicaments: Examples 1 - 9

[0084] Test Crop: Tomato

[0085] Control Object: Meloidogyne incognita

[0086] Dosage of Active Ingredient: 1.5 kg / ha

[0087] Investigation Method for Disease Index and Control Efficacy:

[0088] The diseased roots of tomato root knots were crushed and evenly mixed with 9 times the weight of wet sand to prepare a Meloidogyne incognita inoculum. When planting tomatoes, it was applied into the holes at a rate of about 180 kg·hm -2 for artificial inoculation. There were two application methods. One was single-plant hole application of the granule before planting tomatoes. The other was after planting, watering to slow seedling growth, and performing medicament root irrigation 7 days after planting. No other nematode medicaments were applied during the test period.

[0089] One month after application, the plant height, stem diameter, and chlorophyll content (measured with a chlorophyll meter) of tomatoes in each treatment were investigated. Two months after application, the damage caused by Meloidogyne incognita was investigated. Grading method for the damage of Meloidogyne incognita: Grade 0: Healthy seedlings without disease (no root knots on the roots); Grade 1: Root knots on less than 10% of the roots, and the root knots are not connected to each other; Grade 2: Root knots on 11% - 30% of the roots, and only a small number of root knots are connected to each other; Grade 3: Root knots on 31% - 50% of the roots, and less than half of the root knots are connected to each other; Grade 4: Root knots on 51% - 75% of the roots, more than half of the root knots are connected to each other, and some main and lateral roots become thick and deformed; Grade 5: Root knots on more than 75% of the roots, and they are connected to each other, and most main and lateral roots become thick and deformed.

[0090]

[0091]

[0092] At the harvest stage, the single fruit weight, yield, and sugar content of tomatoes were measured.

[0093] Test results:

[0094] Table 4 Field control efficacy of nematode-killing compositions of Examples 1-9 against Meloidogyne incognita on tomatoes and plant growth

[0095]

[0096]

[0097] Table 5 Field yields and fruit qualities of nematode-killing compositions of Examples 1-9 against Meloidogyne incognita on tomatoes

[0098]

[0099] As can be seen from Table 4 and Table 5, after the trifloxystrobin is compounded with each medicament, the control effect on root-knot nematodes can be significantly improved, the plant height, stem diameter, chlorophyll content, etc. of tomato plants can be increased, the tomato yield can be increased, and the tomato quality can be improved.

[0100] Test Example 4 Field efficacy test

[0101] Test medicaments: Examples 1-9

[0102] Test crop: Potato

[0103] Control object: Ditylenchus destructor

[0104] Dosage of active ingredient used: 1.5 kg / ha

[0105] Investigation method for disease index and control efficacy:

[0106] The diseased potato tubers of potato stem nematode disease were crushed and mixed evenly with 9 times the weight of wet sand to prepare a stem nematode disease inoculum. When planting potatoes, it was applied into the holes at a rate of about 150 kg·hm -2 for artificial inoculation. When planting potatoes, after the water seeped, the treated medicament was mixed evenly with fine sand and applied in holes. No other pesticide treatment was carried out during the test period.

[0107] During the potato harvest period, the damage caused by Ditylenchus destructor was investigated. Grading method for damage caused by Ditylenchus destructor: Grade 0, no damage; Grade 1, the potato tuber is damaged and the diseased area accounts for less than 25% of the potato tuber area; Grade 2: the diseased area accounts for 25%-50% of the potato tuber area; Grade 3: the diseased area accounts for 50%-75% of the potato tuber area; Grade 4: the diseased area accounts for more than 75% of the potato tuber area.

[0108]

[0109]

[0110] Test results:

[0111] Table 6 Field control efficacy of the nematicidal compositions of Examples 1-9 against Ditylenchus destructor on potatoes

[0112]

[0113]

[0114] As can be seen from Table 6, after the compounding of ethoprophos with each medicament, the control efficacy against potato stem nematodes can be significantly improved.

[0115] Test Example 5 Field efficacy test

[0116] Test medicaments: Examples 1-9

[0117] Test crop: soybean

[0118] Target for control: cyst nematode

[0119] Dosage of active ingredient: 1.5 kg / ha

[0120] Method for investigating disease index and control efficacy:

[0121] Soybean roots were retrieved from the plot infected with soybean cyst nematode, crushed and mixed evenly with 9 times the weight of wet sand to prepare the inoculum of cyst nematode disease. Before sowing soybeans, it was applied into the holes at a rate of about 150 kg·hm -2 for artificial inoculation. When sowing soybeans, the treated medicament was mixed evenly with fine sand and applied into the holes, and no medicament application treatment was carried out during the test period.

[0122] 50 days after sowing, 5-point sampling was adopted. At each point, 2 complete soybean plant roots and 300 g of soil around the roots were taken to investigate the number of cysts of soybean cyst nematode in the roots and the soil around the roots and the total number of second-stage (J2), third-stage (J3), and fourth-stage (J4) larvae in the roots.

[0123] Test results:

[0124] Table 7 Field control efficacy of the nematicidal compositions of Examples 1-9 against cyst nematode on soybeans

[0125]

[0126] As can be seen from Table 7, after the compounding of ethoprophos with each medicament, the control efficacy against soybean cyst nematode can be significantly improved.

[0127] From the application of the medicine to the end of the investigation, no visible phytotoxic symptoms were found in the crops treated with the preparations of each example, and the crops grew well after the application of the medicine, indicating that the thifluzamide composition can effectively control the damage of root-knot nematodes. It can be judged that under the same control effect, the dosage of the medicine used is less than that of the control agent, indicating that the present invention can effectively reduce the amount of pesticide used, reduce the production cost of crops, increase the crop yield, reduce the pesticide residue on crops at the same time, and reduce the degree of environmental pollution.

[0128] The above describes the embodiments of the present invention. However, the present invention is not limited to the above embodiments. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. A pesticide composition, the pesticide composition comprising component A and component B; Among them, Component A is trans-aconitic acid; Component B is ethoprophos; The mass ratio of component A to component B is 2:

1.

2. The pesticide composition according to claim 1, wherein The weight percentage content of component A and component B in the pesticide composition is 1-95%.

3. The pesticide composition according to claim 2, wherein The weight percentage content of component A and component B in the pesticide composition is 10-75%.

4. The pesticide composition according to claim 3, wherein The weight percentage content of component A and component B in the pesticide composition is 15-50%.

5. The pesticidal composition according to any one of claims 1-4, characterized in that, The pesticide composition further comprises agriculturally acceptable adjuvants and / or carriers.

6. The pesticidal composition according to claim 5, wherein, The adjuvants and / or carriers include fillers, surfactants.

7. The pesticide composition according to claim 5, characterized in that, The adjuvants and / or carriers include one, two or more of dispersants, wetting agents, emulsifiers, solvents, thickeners, antifreezes, binders, disintegrants, foaming agents, defoaming agents, penetrants, stabilizers, carriers.

8. The pesticidal composition according to any one of claims 1 to 4, characterized in that, The dosage form of the pesticide composition is selected from liquid dosage forms, solid dosage forms.

9. The pesticidal composition according to claim 8, characterized in that, The dosage form of the pesticide composition is selected from soluble solutions, emulsifiable concentrates, emulsions, aqueous solutions, granules.

10. The pesticide composition according to claim 9, wherein The dosage form of the pesticide composition is granules.

11. The pesticidal composition according to claim 10, wherein The granules comprise the following components: component B, trans-aconitic acid, alkylnaphthalenesulfonate, fatty alcohol polyoxyethylene ether sulfate, silica white, polyvinyl alcohol, ammonium sulfate, magnesium stearate, kaolin.

12. The pesticidal composition according to claim 11, characterized in that, The granules comprise the following components: component B is 5 parts, trans-aconitic acid 10 parts, alkylnaphthalenesulfonate 3 parts, fatty alcohol polyoxyethylene ether sulfate 2 parts, silica white 5 parts, polyvinyl alcohol 2 parts, ammonium sulfate 5 parts, magnesium stearate 5 parts, kaolin 63 parts.

13. The pesticide composition according to claim 9, wherein The dosage form of the pesticide composition is soluble solution.

14. The pesticidal composition according to claim 13, characterized in that, The soluble solution comprises the following components: component B, trans-aconitic acid, secondary alcohol polyoxyethylene ether, polyoxyethylene alkylamine, propylene glycol, N-methylpyrrolidone.

15. The pesticide composition according to claim 14, characterized in that, The soluble solution comprises the following components: component B 10 parts, trans-aconitic acid 20 parts, secondary alcohol polyoxyethylene ether 8 parts, polyoxyethylene alkylamine 1 part, propylene glycol 20 parts, N-methylpyrrolidone 41 parts.

16. The pesticide composition according to claim 9, wherein The dosage form of the pesticide composition is emulsifiable concentrate.

17. The pesticidal composition according to claim 16, characterized in that, The emulsifiable concentrate comprises the following components: component B, trans-aconitic acid, fatty alcohol polyoxyethylene ether, triethanolamine salt of triphenylethylphenol polyoxyethylene ether phosphate, cyclohexanone, absolute ethanol, solvent oil.

18. The pesticide composition according to claim 17, wherein The emulsifiable concentrate comprises the following components: component B 10 parts, trans-aconitic acid 20 parts, fatty alcohol polyoxyethylene ether 6 parts, triethanolamine salt of triphenylethylphenol polyoxyethylene ether phosphate 2 parts, cyclohexanone 20 parts, absolute ethanol 5 parts, solvent oil 37 parts.

19. The use of the pesticide composition according to any one of claims 1-18, the pesticide composition is used for controlling plant diseases and pests.

20. The use according to claim 19, characterized in that, The pesticide composition is used for controlling plant nematodes.

21. The use according to claim 20, wherein Plant nematodes include root-knot nematodes, stem nematodes, cyst nematodes, root-lesion nematodes and Bursaphelenchus xylophilus.

22. The use according to claim 19, characterized in that, The pesticidal composition is subjected to soil treatment during the seedling stage of plants or before transplanting of seedlings.

23. The use according to claim 19, characterized in that, The plants include vegetables, fruit trees, economic crops and garden trees.

24. The use according to claim 23, characterized in that, The vegetables are selected from: tomato, cucumber, loofah, gourd, pea, sweet pepper, chili pepper, lettuce, potato, celery; The fruit trees are selected from: citrus, orange, grapefruit, lemon, banana, sugarcane, mango, pineapple, litchi, guava, durian; The economic crops are selected from: cotton, cocoa, coffee, peanut, tobacco, corn, soybean; The garden trees are selected from: fir trees, pine trees.

25. The method for preparing the pesticidal composition according to any one of claims 1-18 is prepared by a conventional method in the pesticidal field.

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