A multifunctional natural sunscreen from hair sources

Abstract

A multifunctional natural sunscreen from hair is disclosed. A sunscreen from hair, the components of which include hair particles loaded with octocrylene. Both in vitro and in vivo experimental results show that the hair particles loaded with octocrylene can effectively protect the skin from ultraviolet radiation, and the hair particles loaded with octocrylene also retain the antioxidant capacity of hair melanin, which can prevent oxidative damage caused by ultraviolet radiation. In addition, the unique shape of the hair particles loaded with octocrylene prevents them from penetrating the skin, thereby avoiding potential toxicity. The present application provides a clever strategy for designing and developing sunscreens from natural substances.

Description

TECHNICAL FIELD

[0001] The present application belongs to the technical field of cosmetics, and particularly relates to a multifunctional natural sunscreen agent from hair. BACKGROUND

[0002] Excessive ultraviolet radiation can cause various skin problems, such as skin phototoxicity, photoaging, photocarcinogenesis, etc. For example, UVA can penetrate the skin surface layer, causing skin photoaging and DNA mutation, and UVB can induce pyrimidine dimers to form cis-cyclobutane pyrimidine dimers (CPD), directly causing DNA damage. Exposure of the skin to ultraviolet light significantly increases the production of reactive oxygen species (ROS), thereby triggering various adverse biological and metabolic reactions, including inflammation and premature skin aging. In addition, the production of reactive oxygen species also activates various matrix metalloproteinases, which damage collagen and other dermal matrix proteins, thereby accelerating skin aging. Ninety percent of human skin aging is closely related to ultraviolet radiation, and long-term exposure to ultraviolet light can accelerate the occurrence and development of melanoma and non-melanoma skin cancer. Studies have shown that the incidence of melanoma and non-melanoma skin cancer significantly increases with the extension of ultraviolet irradiation time and the increase of skin sensitivity to sunlight. Therefore, photoprotection of the skin has become the focus of public attention, which is particularly important for people living in low-latitude, high-altitude, low-ozone concentration, etc. sunlight-rich areas.

[0003] Sunscreen is favored by people due to its good protective effect and convenience of use. At present, the sunscreens on the market are mainly divided into chemical sunscreens and physical sunscreens. The main components of chemical sunscreens are usually organic molecules, such as cinnamic acid, p-aminobenzoic acid, dihydroxyacetone, etc. Although they can provide effective ultraviolet protection, photoactivation of ROS production can cause irreversible damage to the skin. In addition, these lipophilic organic ultraviolet filters can also penetrate deep into the skin, causing harm to the human body. Physical sunscreens are mainly composed of inorganic materials such as titanium dioxide (TiO 2) and zinc oxide (ZnO), which can inhibit skin penetration to some extent, but may produce more ROS after ultraviolet irradiation. In order to avoid potential threats in commercial sunscreens, a series of improvements have been made, for example, ultraviolet absorbing molecules are wrapped in biocompatible polymers or nanoparticles (liposome nanoparticles, polylactic acid nanoparticles, silica nanoparticles, etc.) to reduce their toxicity; in addition, coating inorganic particles with cyclodextrin and biocompatible materials such as polymers is also an effective method to reduce the risk of sunscreen. These studies show that only a slight improvement has been made in minimizing the negative effects of traditional sunscreens, and the fundamental problem has not been solved, and the side effects of such products will still pose a threat to human health. SUMMARY

[0004] In order to overcome the above deficiencies of the prior art, the present application provides a hair-derived sunscreen.

[0005] In order to achieve the above-mentioned object, the technical scheme adopted by the present application is:

[0006] The hair-derived sunscreen comprises hair-derived particles (HDPs) loaded with octocrylene.

[0007] In addition to the hair-derived particles (HDPs) loaded with octocrylene, the components of the hair-derived sunscreen of the present application can also include other commonly used components of sunscreen; the content of the hair-derived particles (HDPs) loaded with octocrylene in the hair-derived sunscreen of the present application can be adjusted according to actual conditions.

[0008] Natural selection and evolution have provided a good material to resist ultraviolet radiation. Melanin is a unique pigment widely present in nature (such as animals, plants and microorganisms), which can protect organisms from ultraviolet damage. By mimicking natural melanin, melanin analogues (polydopamine, polyamino acids, etc.) have been developed for ultraviolet protection, which all have considerable light protection performance. Melanin usually exists in the form of shuttle particles in human hair. In the present application, human hair-derived particles (HDPs) are extracted, which are composed of melanin and keratin. The natural ultraviolet protection effect and the ability to regulate redox balance of melanin make HDPs effective in preventing ultraviolet radiation and scavenging ROS. In addition, the surface porosity and unique spindle structure of HDPs allow them to load drugs and reside on the skin surface without penetrating deep into the skin. After loading the classic sunscreen ultraviolet filter octocrylene, Oct@HDPs are prepared, and Oct@HDPs are used as a multifunctional sunscreen. On the one hand, due to the addition of octocrylene, the ultraviolet capacity of Oct@HDPs is further improved compared with HDPs alone. On the other hand, Oct@HDPs containing melanin effectively inhibit the ROS production induced by photoactivated ultraviolet of ultraviolet filters, avoiding skin damage. At the same time, the special structure of Oct@HDPs allows them to only reside on the skin surface without penetrating deep into the skin, effectively avoiding the potential toxicity of ultraviolet filters in sunscreen applications.

[0009] Preferably, the length of the hair-derived particles loaded with octocrylene is 600-1200 nm; further preferably, the length of the hair-derived particles loaded with octocrylene is 700-1100 nm.

[0010] Preferably, the pore size of the hair-derived particles loaded with octocrylene is 2-15 nm.

[0011] Preferably, the loading amount of octreotide in the octreotide-loaded hair particle is 120-150 mg / g; further preferably, the loading amount of octreotide in the octreotide-loaded hair particle is 130-145 mg / g; in some specific embodiments of the present application, the loading amount of octreotide in the octreotide-loaded hair particle is 138 mg / g.

[0012] Preferably, the hair particle carrier of the octreotide-loaded hair particle is from human hair; further preferably, the hair particle carrier of the octreotide-loaded hair particle is from Asian hair.

[0013] Preferably, the preparation method of the octreotide-loaded hair particle is as follows:

[0014] (1) mixing hair with NaOH solution, heating, filtering to obtain filtrate, centrifuging, transferring the obtained supernatant to an ultrafiltration tube for ultrafiltration, washing with water to obtain hair particles;

[0015] (2) preparing the hair particles in step (1) into an aqueous solution, adding an octreotide solution, stirring after ultrasonic treatment, and obtaining the octreotide-loaded hair particles after centrifugation.

[0016] Further preferably, in step (1), the concentration of the NaOH solution is 0.8-1.2 mol / L; further preferably, the concentration of the NaOH solution is 0.9-1.1 mol / L.

[0017] Further preferably, in step (1), the heating temperature is 90-100℃, and the heating time is 10-20 min; further preferably, in step (1), the heating temperature is 92-98℃, and the heating time is 12-18 min.

[0018] Further preferably, in step (1), after the hair is mixed with the NaOH solution and heated, a buffer solution is used for dilution and neutralization; the buffer solution is a PBS buffer solution.

[0019] Further preferably, in step (1), the specification of the ultrafiltration tube is 100 kDa.

[0020] Further preferably, in step (2), the concentration of the aqueous solution after the hair particles are prepared is 1-3 mg / L.

[0021] Further preferably, in step (2), the solvent of the octreotide solution is dimethyl sulfoxide (DMSO), and the concentration of the octreotide solution is 4-6 mg / L.

[0022] Further preferably, in step (2), the volume ratio of the aqueous solution of the hair particles to the solution of octocrylene is 1 : (18-22).

[0023] Further preferably, in step (2), the time of the ultrasonication is 10-20 min.

[0024] Further preferably, in step (2), the time of the stirring is 20-28 h; further more preferably, in step (2), the time of the stirring is 22-26 h.

[0025] Compared with the prior art, the present application has the following beneficial effects:

[0026] Both in vitro and in vivo experimental results show that Oct@HDPs can effectively protect the skin from the damage of ultraviolet radiation, and Oct@HDPs also retain the antioxidant ability of hair melanin, which can prevent oxidative damage caused by ultraviolet radiation. In addition, the unique shape of Oct@HDPs prevents them from penetrating the skin, thereby avoiding potential toxicity. The present application provides a clever strategy for designing and developing sunscreen from natural substances. BRIEF DESCRIPTION OF DRAWINGS

[0027] Figure 1 TEM image of HDPs prepared for the example;

[0028] Figure 2 Hydrodynamic diameter graph of HDPs prepared for the example;

[0029] Figure 3 Pore size distribution graph of HDPs prepared for the example;

[0030] Figure 4 UV-vis absorption spectrum graph of octocrylene, HDPs and Oct@HDPs;

[0031] Figure 5 ROS generation graph after ultraviolet irradiation;

[0032] Figure 6 Quantitative analysis graph of ROS after ultraviolet irradiation;

[0033] Figure 7 Cell activity graph when HDPs and Oct@HDPs act;

[0034] Figure 8 Cell activity graph when irradiated by ultraviolet light or non-ultraviolet light;

[0035] Figure 9 In vitro skin fluorescence microscope observation graph;

[0036] Figure 10 Figure 9 ​Quantitative analysis of average fluorescence intensity at different depths;

[0037] Figure 11 Microscopic observation of H&E staining of mouse dorsal epidermis after UV irradiation;

[0038] Figure 12 For Figure 11 Relative epidermis thickness in each group;

[0039] Figure 13 Microscopic observation of Masson staining of mouse dorsal epidermis after UV irradiation;

[0040] Figure 14 For Figure 13 Relative keratin area in each group. DETAILED DESCRIPTION

[0041] The specific embodiments of the present application will be further described below. It should be noted that the description of these embodiments is used to help understand the present application, but does not constitute a limitation on the present application. In addition, the technical features involved in each embodiment of the present application described below can be combined with each other as long as they do not conflict with each other.

[0042] The experimental methods in the following examples are all conventional methods unless otherwise specified, and the test materials used in the following examples are all commercially available unless otherwise specified.

[0043] Example 1

[0044] This embodiment provides a preparation method of Oct@HDPs, which specifically comprises the following steps:

[0045] HDPs are prepared by hydrolysis method. First, take 5 g of undyed human hair sample, wash the sample with deionized water for three times, and vacuum freeze dry for 24 h. Then, grind the freeze-dried hair sample into fragments at 4℃, and dissolve the ground sample in 1 molL -1 of NaOH solution (20 mL) and heat at 95℃ for 15 min. After the solution is cooled, dilute it in 200 mL of PBS, then remove the incompletely dissolved hair fragments by filtering with flannel, and further remove the hair fragments by centrifugation at 600g for 10 min. Transfer the obtained supernatant to a 100 kDa ultrafiltration tube for ultrafiltration (10000g, 10 min), wash with deionized water for 3 times, and store the obtained precipitate at 4℃ after re-dissolving with deionized water.

[0046] Octocrylene is loaded into HDPs by hydrophobic interaction. Take 0.5 mL of octocrylene (5 mgmL -1Add dropwise to 10 mL of HDPs solution (2 mg / mL) in DMSO. -1 In deionized water, sonicate for 15 minutes. Then, stir for 24 hours to form Oct@HDPs, centrifuge at 1000g and wash three times with deionized water, then store at 4°C for later use.

[0047] The TEM images of the HDPs prepared in this embodiment are attached. Figure 1 As shown, the scale bar is 1 μm. HDPs exhibit a uniform spindle-shaped structure with an average length of approximately 800 nm and an average width of approximately 200 nm.

[0048] The hydration dynamic diameters of HDPs are shown in the attached figure. Figure 2 As shown. The hydrodynamic diameter of HDPs was measured by dynamic light scattering (DLS), which further confirmed the unique shape of HDPs, with two peaks at 250 nm and 1000 nm, corresponding to the width and length of the particles, respectively.

[0049] The pore size distribution of HDPs is shown in the attached figure. Figure 3 As shown in the figure. Nitrogen adsorption analysis revealed that HDPs have a non-uniform mesoporous structure with pore sizes ranging from 3 nm to 10 nm, indicating their potential as drug carriers.

[0050] The UV-vis absorption spectra of octocrylene, HDPs, and Oct@HDPs are attached. Figure 4 As shown, a characteristic absorption peak is observed at 300 nm. The UV-Vis absorption spectra of HDPs were measured using a UV spectrophotometer to determine their UV protection capability.

[0051] To further confirm the ROS scavenging ability of Oct@HDPs, the ROS generation of HDPs and Oct@HDPs after UV irradiation was investigated. Commercial sunscreen, inorganic UV-filtered titanium dioxide nanoparticles (50 nm), and synthetic melanin nanoparticles (PDANPs, 200 nm) were used as controls. ROS generation after UV irradiation was detected using a DHR (a widely used ROS probe), with 2 mL of octocrylene emulsion (20 μg / mL) as a control. -1 Dopamine nanoparticles (PDA NPs, 0.1 mg / mL) -1 Nano titanium dioxide (0.1 mg / mL) -1 HDPs (0.1 mg / mL) -1 ) and OCT@HDPs (0.1 mg mL) -1 The solutions were incubated in quartz tubes with 50 μL of DHR (dissolved in 330 μM ethanol) for 20 minutes. They were then irradiated with a UV lamp (UVB, 8W) for 2 hours, and the fluorescence intensity of these solutions was measured at excitation / emission wavelengths of 485 / 538 nm.

[0052] The ingredients of commercial sunscreen are: water, octocrylene, C12-15 alcohol benzoate, titanium dioxide, butylene glycol, glycerin, potassium cetyl phosphate, butyl methoxydibenzoylmethane, ethylhexyl methoxyphenyl trioleate, cyclopentasiloxane, dicaprylyl carbonate, chamomile flower extract, aloe barbadensis leaf extract, scutellaria baicalensis root extract, glycyrrhiza glabra root extract, glyceryl stearate, PEG-100 stearate, cetearyl alcohol, ascorbyl acetate, magnesium aluminum silicate, hydroxyethyl acrylate / sodium acryloyldimethyl taurate copolymer, ammonium acryloyldimethyltaurate / VP copolymer, squalane, xanthan gum, propylene glycol, polysorbate-60, maltodextrin, sorbitan isostearate, sodium hydroxide, imidazolidinyl urea, methylisothiazolinone, ethylisothiazolinone, disodium EDTA, fragrance.

[0053] The PDANPs are artificial synthetic melanin nanoparticles, which are used as a control for the natural hair nanoparticle HDPs. The preparation method is as follows: 2.5 mL of ammonia water, 40 mL of ethanol and 90 mL of deionized water are mixed uniformly, stirred at room temperature for 30 min, then 10 mL of dopamine hydrochloride aqueous solution (50 mg / mL) is slowly added dropwise into the above solution, reacted under stirring for 24 h, then the PDA is centrifuged and washed with water for 3 times.

[0054] As shown in Figure 5 Figure 2, after UV irradiation, the fluorescence intensity of the octocrylene group, the TiO2 group and the commercial sunscreen group is obviously stronger than that of the control group, indicating that the three components will produce a large amount of ROS under UV irradiation. The ROS level of the PDA and HDPs groups is obviously reduced, and the DHR fluorescence intensity is obviously reduced, indicating that the melanin in PDA and HDPs can act as an effective antioxidant to eliminate the generated ROS. Even if loaded with octocrylene, HDPs can effectively eliminate the ROS generated by octocrylene. Quantitative analysis also shows that the fluorescence intensity of Oct@HDPs is reduced by 38.8% and 76.8% compared with the control group and the octocrylene group, respectively. Figure 6 These data show that Oct@HDPs have strong ROS scavenging ability and can effectively eliminate the ROS generated by the activation of UV filtering light.

[0055] Example 2

[0056] In vitro cytotoxicity and UV-induced intracellular ROS production experiment

[0057] Experimental procedure: NIH3T3 cells were inoculated in a 96-well plate (1 x 10 4 cells per well). After 24 h of culture, different concentrations of HDPs and Oct@HDPs (1.5-200 μg / mL) were added to the cells, and the cells were irradiated with UV light for 30 min. After 24 h of culture, the DHR fluorescence intensity of the cells was detected. -1Incubate for another 24 hours. Cell viability was assessed using a CCK8 assay kit. The experiment was performed in six replicates. Results are as follows: Figure 7 As shown.

[0058] like Figure 7 As shown, neither HDPs nor Oct@HDPs significantly inhibited the activity of 3T3 cells, and even at high concentrations (200 μg / mL), they maintained high viability levels. Figure 8 As shown, the experimental concentration was octocrylene emulsion (20 μg / mL). -1 Dopamine nanoparticles (PDANPs, 0.1 mg / mL) -1 Nano titanium dioxide (0.1 mg / mL) -1 HDPs (0.1 mg / mL) -1 ) and Oct@HDPs (0.1 mg / mL) -1 The specific experimental procedure was as follows: 3T3 cells were seeded in 96-well plates (1×10⁶ cells per well). 4 Cells were cultured for 24 hours and then incubated with various drugs (PBS, octocrylene emulsion, sunscreen, PDANPs, TiO2NPs, HDPs, and Oct@HDPs) for 4 hours. The cells were then irradiated with a UV lamp (UVB, 8W) for 15 minutes and cultured for another 24 hours. Cell viability was assessed using a CCK8 assay kit. Each experiment was performed in 6 replicates.

[0059] Without UV irradiation, the impact on cell viability was negligible in all groups except for those treated with commercial sunscreen. However, under UV irradiation, octocrylene, commercial sunscreen, and TiO2 all exhibited significantly higher cytotoxicity than the control group, indicating the generation of harmful ROS under UV irradiation. This phenomenon was not observed in the PDA, HDPs, and Oct@HDPs groups, suggesting that these particles possess the ability to scavenge ROS. These results confirm that HDPs can protect cells from UV damage by scavenging ROS generated during irradiation and demonstrate effective UV protection.

[0060] Example 3

[0061] Oct@HDPs Ex vivo skin penetration experiment

[0062] Procedure: Fresh pigskin was obtained from a nearby market and frozen at -20°C before use. Before use, the pigskin was thawed, washed with PBS, and cut into 1.5×1.5cm pieces. Then, RBITC-labeled TiO2, PDA, and HDPs (0.1 mg / mL) were added. -1) Topically applied on skin samples. RBITC in PBS was used as a control in this experiment. After incubation in a humidity chamber at 32°C for 8 h, the skin samples were rinsed with PBS for 3 times and then dried. Subsequently, the skin tissues were fixed in 4% paraformaldehyde and cryosectioned. The sectioned tissues were observed under a fluorescence microscope and the images were analyzed using ImageJ software, and the results are shown in Figure 9

[0063] Figure 9 Represented incubation on pig skin in a humidity chamber at 32°C for 6 h (n = 3), scale bar 200 pm. Figure 10 Figure 9 Quantitative analysis of the average fluorescence intensity at different depths (n = 3). Data are expressed as mean ± standard deviation. The significance between groups was calculated using analysis of variance and Tukey's post-hoc test. ***P < 0.05, **P < 0.01, ***P < 0.001, ns: not significant.

[0064] To visually observe the penetration, TiO2, PDA and HDPs were labeled with rhodamine B isothiocyanate (RBITC) respectively, and free RBITC was used as a control to simulate free organic UV filters. Fluorescence imaging technology was used to observe the penetration of particles in each group of pig skin after incubation for 8 h. As shown in Figure 9 , free RBITC was distributed in the entire skin tissue including the epidermis and dermis layers, and the distribution decreased with increasing depth. In contrast, the fluorescence signal in the deep layer of the skin tissue in the HDPs group was negligible, and almost all the particles were distributed on the skin surface, which may be due to the larger size of HDPs, indicating that the skin retention of HDPs was significantly higher and did not penetrate into the deep layer of the skin. It is worth noting that when the particle size becomes nanoscale (including TiO2 and PDA), most of the particles penetrate the epidermis layer of the skin, while a small part of the particles can still be detected in the dermis layer, which can be seen from the fluorescence signal distribution in the fluorescence image of the skin tissue. Quantitative data also showed that both free dye and nanoparticles showed obvious penetration into the dermis layer, while HDPs did not penetrate the dermis layer Figure 10 . These results suggest that HDPs can maintain long-term skin retention without penetrating the deep layer of the skin, and the very low rhodamine fluorescence intensity observed in the deep layer of the skin suggests that Oct@HDPs formed after loading octocrylene into HDPs may have effective penetration prevention ability of UV filters.

[0065] Example 4

[0066] In vivo UV protection efficiency

[0067] ​​Experimental Procedure: Six-week-old BALB / c mice were anesthetized by intraperitoneal injection of chloral hydrate (4%, 200 μL / mouse), their back hair was shaved, and treated with depilatory cream. Three days later, the back skin was washed with 70% alcohol and divided into 5 small pieces, each measuring 1 × 1 cm. One piece was treated with PBS as a control, and the other piece was treated with HDPs (0.1 mg / mL). -1 ), TiO2NPs (CAS:13463-67-7) (0.1mgmL -1 ), sunscreen and Oct@HDPs (0.1 mg / mL) -1 The mice were then treated with a black covering. The dorsal skin was then exposed to a UVB lamp (8W) for 30 minutes, and the remaining skin was covered with a black covering. Three days later, the mice were sacrificed, and skin tissue was collected for histological analysis. The skin tissue was fixed with 4% paraformaldehyde and cut into serial sections. The tissue sections were then stained with H&E and Masson's trichomorphs and observed under a light microscope. Epidermal thickness and keratin content were analyzed using ImageJ software.

[0068] Figure 11 H&E staining of the dorsal epidermis of mice after ultraviolet irradiation (n=3), scale bar: 100 μm. Figure 12 The relative epidermal thickness of each group (n=3) was analyzed. Figure 11 The image is used to calculate the epidermal thickness. Figure 13 Masson staining of the dorsal epidermis of mice after ultraviolet irradiation (n=3), scale bar 100μm. Figure 14 The relative keratin area of ​​each group (n=3) was analyzed. Figure 13 Keratin area was calculated, and data are expressed as mean ± standard deviation. Analysis of variance and Tukey's post-hoc test were used to calculate significance between groups. *p<0.05, ****p<0.0001.

[0069] from Figure 11 The images show that the skin of PBS-treated mice exposed to ultraviolet light was significantly thicker compared to that of normal mice. Quantification of epidermal thickness from the images indicates that the epidermal thickness in the PBS-treated group after ultraviolet light exposure was almost twice that of normal skin. Figure 12However, the epidermal hypertrophic effect induced by UV irradiation was significantly weakened by the treatment of commercial sunscreen, TiO2 or HDPs. Quantitative data showed that the epidermal thickness of commercial sunscreen, TiO2 and HDPs treated groups decreased by 77.6%, 53.7% and 77.8% respectively compared with PBS treated group. Notably, the skin protected by Oct@HDPs had a significant inhibition effect on the epidermal hyperplasia induced by UV, and the epidermal thickness was the same as normal skin, indicating that Oct@HDPs had high UV protection performance. Further analysis of Masson staining of mouse skin found that the keratin content of PBS treated group was about three times that of normal skin after UV irradiation. In contrast, the keratin hyperplasia effect was significantly alleviated after the treatment of commercial sunscreen, TiO2 or HDPs, and the detected keratin content increased by 75%, 68% and 30% respectively; while no obvious keratin hyperplasia phenomenon was observed in the skin protected by Oct@HDPs, which could effectively prevent skin keratinization. Consistent with the results of H&E staining, Masson staining also confirmed that Oct@HDPs had good UV protection efficiency in vivo.

[0070] The above detailed the embodiments of the present application, but the present application is not limited to the described embodiments. For those skilled in the art, various changes, modifications, replacements and variations of the embodiments can be made without departing from the principles and spirits of the present application, and still fall within the protection scope of the present application.

Claims

1. A hair-derived sunscreen agent, characterized in that, The hair-derived sunscreen comprises hair particles loaded with octocrylene. The hair particles loaded with octocrylene have a length of 600-1200 nm. The pore size of the hair particles loaded with octocrylene is 2-15 nm; The octocrylene loading of the hair particles containing octocrylene is 120-150 mg / g; The method for preparing the hair particles loaded with octocrylene is as follows: (1) Mix hair with NaOH solution, heat, filter to obtain filtrate, centrifuge, transfer the supernatant to ultrafiltration tube for ultrafiltration, wash with water to obtain hair particles; (2) Prepare an aqueous solution of the hair particles described in step (1), add octocrylene solution, sonicate and stir, and centrifuge to obtain the hair particles loaded with octocrylene. In step (1), the concentration of the NaOH solution is 0.8-1.2 mol / L; In step (1), the heating temperature is 90-100℃ and the heating time is 10-20 min; In step (2), the stirring time is 20-28 hours; Oct@HDPs are formed by loading octocrylene into hair particles (HDPs). Oct@HDPs have a strong ROS removal capability and can effectively remove ROS generated by UV-filtered light activation. HDPs can protect cells from UV damage by clearing ROS generated during irradiation and have shown effective UV protection capabilities. HDPs can maintain long-term skin retention without penetrating deep into the skin, and extremely low Rhodamine fluorescence intensity is observed in deep skin layers. Oct@HDPs have the ability to effectively prevent UV filter penetration.

2. The hair-derived sunscreen agent according to claim 1, characterized in that, The hair particle carrier loaded with octocrylene is derived from human hair.

3. The hair-derived sunscreen agent according to claim 2, characterized in that, The hair particle carrier loaded with octocrylene is derived from Asian hair.

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