Control method, device, system, computer device and storage medium for perfusion

Abstract

The application relates to a perfusion control method, device, system, computer equipment and storage medium, wherein the method comprises the following steps: determining whether a perfusion system and a weighing system are associated; entering an association mode when the perfusion system and the weighing system are associated; acquiring the liquid weight of cell culture solution through the weighing system in the association mode; determining a first control signal of a perfusion pump according to the liquid weight, a preset perfusion weight and a preset dead zone weight; processing a preset perfusion flow rate to obtain a first target flow rate based on the flow rate limitation condition of the perfusion pump; and controlling the perfusion of the cell culture solution according to the first control signal and the first target flow rate. Through the application, the problem that data tracking and recording cannot be realized and the perfusion effect is influenced by human factors is solved, the perfusion system and the weighing system are associated, data tracking and recording can be realized, and the influence of human factors on the perfusion effect is reduced.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of automation control, and in particular to a perfusion control method, device, system, computer equipment and storage medium. BACKGROUND

[0002] The application of perfusion culture technology in cell culture is that two perfusion pumps are responsible for the liquid inlet and outlet of cell culture, constantly updating the culture solution in the reactor and quickly discharging the metabolic products of cells, reducing the accumulation of harmful metabolic waste concentration, providing a relatively stable and good nutrient environment for cells, flexibly changing the survival conditions of cells, improving the high-density survival of cells while continuously harvesting the products contained in the outlet liquid, improving the production rate and the recovery rate of target products.

[0003] In the traditional control scheme of perfusion, the perfusion system is relatively independent of the weighing system of the culture solution, and the data collected by the weighing system needs to be manually input into the perfusion system for association, and then the perfusion control is realized. The problem of this scheme is that it is inconvenient to use and cannot realize data tracking and recording, and there is a human factor affecting the perfusion effect.

[0004] For the problem that the related art cannot realize data tracking and recording and there is a human factor affecting the perfusion effect, there is currently no effective solution. SUMMARY

[0005] A perfusion control method, device, system, computer equipment and storage medium are provided in the embodiment to solve the problem that the related art cannot realize data tracking and recording and there is a human factor affecting the perfusion effect.

[0006] In a first aspect, a perfusion control method is provided in the embodiment, which is applicable to a perfusion control system; the perfusion control system includes a perfusion system and a weighing system; the method includes:

[0007] determining whether the perfusion system and the weighing system are associated;

[0008] when the perfusion system and the weighing system are associated, entering an association mode;

[0009] In the association mode, the liquid weight of the cell culture solution is obtained through the weighing system; the first control signal of the perfusion pump is determined according to the liquid weight, a preset perfusion weight and a preset dead zone weight;

[0010] based on the flow rate limitation condition of the perfusion pump, processing the preset perfusion flow rate to obtain a first target flow rate;

[0011] controlling the perfusion of the cell culture solution according to the first control signal and the first target flow rate.

[0012] In some embodiments, the method further comprises:

[0013] When the perfusion system is not associated with the weighing system, entering a manual mode;

[0014] In the manual mode, processing a preset perfusion flow rate based on a flow rate limit condition of the perfusion pump to obtain a first target flow rate;

[0015] Controlling perfusion of the target cells according to a second control signal of the manual mode and the first target flow rate.

[0016] In some embodiments, the processing of the preset perfusion flow rate based on the flow rate limit condition of the perfusion pump to obtain the first target flow rate comprises:

[0017] Comparing the preset perfusion flow rate with the flow rate limit condition of the perfusion pump;

[0018] When the preset perfusion flow rate is greater than a maximum flow rate of the perfusion pump, setting the maximum flow rate of the perfusion pump as the first target flow rate;

[0019] When the preset perfusion flow rate is less than or equal to the maximum flow rate of the perfusion pump and greater than or equal to a minimum flow rate of the perfusion pump, taking the preset perfusion flow rate as the first target flow rate;

[0020] When the preset perfusion flow rate is less than the minimum flow rate of the perfusion pump, setting the minimum flow rate of the perfusion pump as the first target flow rate and determining a duty cycle corresponding to the first target flow rate based on a duty cycle flow control mode.

[0021] In some embodiments, the duty cycle = the first target flow rate / the minimum flow rate of the perfusion pump*pre-set control fraction.

[0022] In some embodiments, the determining of the first control signal of the perfusion pump according to the liquid weight, a preset perfusion weight and a preset dead zone weight comprises:

[0023] Determining a dead zone range according to the preset perfusion weight and the preset dead zone weight and comparing the liquid weight with the dead zone range;

[0024] When the liquid weight meets the dead zone range, generating an opening signal of the perfusion pump for liquid output and an opening signal of the perfusion pump for liquid input simultaneously.

[0025] In some embodiments, the method further comprises:

[0026] compare the preset perfusion weight with the liquid weight when the liquid weight does not satisfy the dead zone range;

[0027] generate an opening signal of the perfusion pump for outflow when the preset perfusion weight is greater than the liquid weight;

[0028] generate an opening signal of the perfusion pump for inflow when the preset perfusion weight is less than the liquid weight.

[0029] In a second aspect, a control device for perfusion is provided in the embodiments, comprising a determination module, a first entering module, a first processing module, a second processing module and a first perfusion control module;

[0030] The determination module is configured to determine whether the perfusion system and the weighing system are associated.

[0031] The first entering module is configured to enter an associated mode when the perfusion system and the weighing system are associated.

[0032] The first processing module is configured to, in the associated mode, acquire a liquid weight of the cell culture solution by the weighing system, and determine a first control signal of a perfusion pump according to the liquid weight, a preset perfusion weight and a preset dead zone weight.

[0033] The second processing module is configured to process a preset perfusion flow rate to obtain a first target flow rate based on a flow rate limitation condition of the perfusion pump.

[0034] The first perfusion control module is configured to control perfusion of the cell culture solution according to the first control signal and the first target flow rate.

[0035] In a third aspect, a control system for perfusion is provided in the embodiments, comprising a perfusion system and a weighing system associated with the perfusion system.

[0036] The weighing system is connected with a reactor and a control cabinet in the perfusion system respectively, and is configured to acquire a liquid weight of a cell culture solution in the reactor.

[0037] The perfusion system further comprises an inflow pipe, an outflow pipe, an inflow perfusion pump and an outflow perfusion pump; the inflow pipe is connected with the reactor and the inflow perfusion pump, and the outflow pipe is connected with the reactor and the outflow perfusion pump.

[0038] The control cabinet is connected with a control method, a device, a system, a computer device and a storage medium for the inflow perfusion pump and the outflow perfusion pump, and is configured to execute the control method for perfusion in the first aspect.

[0039] In a fourth aspect, a computer device is provided in the present embodiment, comprising a memory, a processor, and a computer program stored in the memory and executable on the processor, and the processor implements the perfusion control method of the first aspect when executing the computer program.

[0040] In a fifth aspect, a storage medium is provided in the present embodiment, and the storage medium stores a computer program, and the computer program is executable on a processor to implement the perfusion control method of the first aspect.

[0041] Compared with the related art, the perfusion control method, device, system, computer device and storage medium provided in the present embodiment determine whether the perfusion system and the weighing system are associated; when the perfusion system and the weighing system are associated, enter the associated mode; in the associated mode, obtain the liquid weight of the cell culture solution through the weighing system; determine the first control signal of the perfusion pump according to the liquid weight, the preset perfusion weight and the preset dead zone weight; process the preset perfusion flow rate based on the flow rate limitation condition of the perfusion pump to obtain the first target flow rate; and control the perfusion of the cell culture solution according to the first control signal and the first target flow rate, which solves the problem that the data tracking and recording cannot be realized and the perfusion effect is affected by human factors when the perfusion is inconvenient to use, and the data tracking and recording can be realized and the influence of human factors on the perfusion effect can be reduced by associating the perfusion system and the weighing system.

[0042] Details of one or more embodiments of the present application are presented in the following drawings and description to make other features, objects and advantages of the present application more apparent. BRIEF DESCRIPTION OF DRAWINGS

[0043] The drawings described herein are intended to provide further understanding of the present application, and form a part of the present application. The illustrative embodiments of the present application and their description serve to explain the present application, and do not constitute an improper limitation of the present application. In the drawings:

[0044] Figure 1 is a hardware structure block diagram of the perfusion system provided in an embodiment of the present application;

[0045] Figure 2 is a flowchart of the perfusion control method provided in an embodiment of the present application;

[0046] Figure 3 is a flowchart of the perfusion control method provided in another embodiment of the present application;

[0047] Figure 4 is a flowchart of the perfusion control method in the associated mode provided in a preferred embodiment of the present application;

[0048] Figure 5is a structural block diagram of a control device of perfusion provided by an embodiment of the present application.

[0049] In the figure: 10, reactor; 20, liquid inlet pipe; 30, liquid outlet pipe; 40, liquid inlet perfusion pump; 50, liquid outlet perfusion pump; 60, control cabinet; 210, determination module; 220, first entering module; 230, first processing module; 240, second processing module; 250, first perfusion control module. DETAILED DESCRIPTION

[0050] In order to more clearly understand the purpose, technical solutions and advantages of the present application, the present application is described and explained below in conjunction with the accompanying drawings and embodiments.

[0051] Unless otherwise defined, technical terms or scientific terms used in the present application shall have the general meaning understood by a person with ordinary skill in the art to which the present application belongs. In the present application, "one", "a", "an", "the", "these" and similar words do not represent a quantitative limitation, and they can be singular or plural. In the present application, the terms "include", "contain", "have" and any variants thereof have the purpose of covering non-exclusive inclusion; for example, a process, method and system, product or device containing a series of steps or modules (units) are not limited to the listed steps or modules (units), but can include steps or modules (units) not listed, or can include other steps or modules (units) inherent to the process, method, product or device. In the present application, the terms "connected", "connected", "coupled" and similar words are not limited to physical or mechanical connections, but can include electrical connections, whether direct or indirect. In the present application, "multiple" means two or more. The association between the associated objects is described by "and / or", which means that there can be three relationships, for example, "A and / or B" can mean that A exists alone, A and B exist together, and B exists alone. In general, the character " / " represents an "or" relationship between the objects before and after it. In the present application, the terms "first", "second", "third" and the like are only used to distinguish similar objects, and do not represent a specific order of the objects.

[0052] The method embodiment provided in the present embodiment can be applied to a perfusion control system, including a perfusion system and a weighing system associated with the perfusion system; wherein the perfusion system is as described above, and the weighing system is as described above. Figure 1As shown, it comprises a reactor 10, a control cabinet 60, an inlet pipe 20, an outlet pipe 30, an inlet perfusion pump 40 and an outlet perfusion pump 50; a weighing system connected with the reactor 10 and the control cabinet 60 in the perfusion system respectively, for collecting the liquid weight of the cell culture solution in the reactor 10; the inlet pipe 20 connects the reactor 10 and the inlet perfusion pump 40, and the outlet pipe 30 connects the reactor 10 and the outlet perfusion pump 50; the control cabinet 60 is connected with the inlet perfusion pump 40 and the outlet perfusion pump 50; and is used for executing the control method of perfusion in each embodiment.

[0053] The reactor 10 can be a jet reactor. The cell culture solution in the reactor 10 is used for culturing cells, and the inlet perfusion pump 40 is responsible for the inlet of the cell culture through the inlet pipe 20, and the outlet perfusion pump 50 is responsible for the outlet of the cell culture through the outlet pipe 30.

[0054] The weighing system can be provided in the reactor 10, which can collect the liquid weight of the cell culture solution in the reactor 10 and transmit the collected liquid weight to the control cabinet 60; the control cabinet 60 is connected with the inlet perfusion pump 40 and the outlet perfusion pump 50, and is used for executing the control method of perfusion in each embodiment to constantly update the cell culture solution in the reactor 10 and quickly discharge the metabolic products of the cells, reduce the accumulation of harmful metabolic waste concentration, provide a relatively stable better nutrient environment for the cells, flexibly change the survival conditions of the cells, improve the high-density survival of the cells while constantly harvesting the products contained in the outlet, and improve the production rate and the recovery rate of the target product.

[0055] The control cabinet 60 is provided with a processor and a memory for storing data, wherein the processor can include but is not limited to a microprocessor MCU or a programmable logic device FPGA processing device. The memory can be used to store computer programs, for example, software programs of application software and modules, such as the computer program corresponding to the temperature control method of the vehicle in the embodiment. The processor executes the computer program stored in the memory to perform various functional applications and data processing, that is, to realize the above-mentioned method. The memory can include a high-speed random access memory, and can also include a non-volatile memory, such as one or more magnetic storage devices, flash memories, or other non-volatile solid-state memories. In some examples, the memory can further include a memory remotely arranged with respect to the processor, and these remote memories can be connected to the terminal through a network. Examples of the above-mentioned network include but are not limited to the Internet, an intranet, a local area network, a mobile communication network, and a combination thereof.

[0056] The system can further include a transmission device for communication function and an input and output device. The transmission device is used to receive or send data via a network. The network includes a wireless network provided by a communication provider of the terminal. In an example, the transmission device includes a network adapter (NIC) which can be connected to other network devices through a base station so as to communicate with the Internet. In an example, the transmission device can be a radio frequency (RF) module which is used to communicate with the Internet in a wireless manner.

[0057] In the embodiment, a control method of perfusion is provided, Figure 2 is a flow chart of the control method of perfusion in the embodiment, as shown in the figure, the flow includes the following steps: Figure 2

[0058] Step S210, determine whether the perfusion system and the weighing system are associated;

[0059] Step S220, when the perfusion system and the weighing system are associated, enter an associated mode;

[0060] Step S230, in the associated mode, obtain the liquid weight of the cell culture solution through the weighing system; determine the first control signal of the perfusion pump according to the liquid weight, a preset perfusion weight and a preset dead zone weight;

[0061] Step S240, process the preset perfusion flow rate to obtain a first target flow rate based on a flow rate limitation condition of the perfusion pump;

[0062] Step S250, control the perfusion of the cell culture solution according to the first control signal and the first target flow rate.

[0063] Specifically, in response to the user operation of the control interface of the perfusion system to select whether the perfusion system and the weighing system are associated; when the association is selected, it can be determined that the perfusion system and the weighing system are associated, and enter the associated mode; when the non-association is selected, it can be determined that the perfusion system and the weighing system are not associated, and enter a manual mode. Wherein, the association of the perfusion system and the weighing system refers to using a set of perfusion control system to realize the tracking and recording of data in the perfusion control system.

[0064] In the associated mode, the perfusion flow rate, the perfusion weight and the dead zone weight can be input in the control interface of the perfusion system, and the liquid weight of the cell culture solution obtained through the weighing system can be combined, so as to automatically control the perfusion of the cell culture solution, without manual operation, thereby avoiding the influence of human factors on the perfusion effect. It needs to be known that the automatic control of the perfusion of the cell culture solution is mainly to control the flow rate of the perfusion and the start and stop of the perfusion pump. ​

[0065] The first control signal of the perfusion pump is determined according to the liquid weight, the preset perfusion weight, and the preset dead zone weight by using a control strategy or a control algorithm. The first control signal includes an opening signal and a closing signal of the liquid inlet perfusion pump, and also includes an opening signal and a closing signal of the liquid outlet perfusion pump. Due to the limitation of the mechanical performance of the perfusion pump, each type of flow pump has a flow rate limiting condition, which includes but is not limited to a maximum flow rate, a minimum flow rate, etc. The preset perfusion flow rate is processed based on the flow rate limiting condition of the perfusion pump, so that the first target flow rate meets the flow rate limiting condition. The control mode of the perfusion pump is different, and the first target flow rate matches the corresponding control signal; if the perfusion pump is a duty cycle control, a duty cycle corresponding to the first target flow rate is determined, and then a corresponding duty cycle control signal is generated. If the perfusion pump is a proportional flow control, a proportional control signal corresponding to the first target flow rate is determined. The first control signal and the first target flow rate can be determined according to actual conditions, which will not be illustrated one by one here.

[0066] After the first control signal and the first target flow rate are determined, the corresponding control signal is converted to control the operation of the two perfusion pumps, so as to control the perfusion of the cell culture solution; thereby the cell culture solution in the reactor is continuously updated and the metabolic products of the cells are quickly discharged, and the accumulation of harmful metabolic waste concentration is reduced.

[0067] Through the above steps, when the perfusion system is associated with the weighing system, the association mode is entered; so as to realize the tracking and recording of data. In the association mode, the liquid weight of the cell culture solution is obtained through the weighing system; the first control signal of the perfusion pump is determined according to the liquid weight, the preset perfusion weight, and the preset dead zone weight; the preset perfusion flow rate is processed based on the flow rate limiting condition of the perfusion pump, and the first target flow rate is obtained; the perfusion of the cell culture solution is controlled according to the first control signal and the first target flow rate, so as to realize the automatic perfusion control, reduce the influence of human factors on the perfusion effect, and solve the problems of inconvenience of use, inability to realize data tracking and recording, and influence of human factors on the perfusion effect.

[0068] In some embodiments, as shown in FIG. 3, Figure 3 The control method of perfusion further includes the following steps:

[0069] Step S320, when the perfusion system is not associated with the weighing system, the manual mode is entered;

[0070] Step S330, in the manual mode, the preset perfusion flow rate is processed based on the flow rate limiting condition of the perfusion pump, and the first target flow rate is obtained;

[0071] Step S340, the perfusion of the target cells is controlled according to the second control signal of the manual mode and the first target flow rate.

[0072] Specifically, the manual mode and the associated mode are two parallel modes. The difference is that in the associated mode, the first control signal is determined by the liquid weight, the preset perfusion weight and the preset dead zone weight; while in the manual mode, the second control signal is determined by responding to the user operating the control interface of the perfusion system; that is, the user controls the opening and closing of the two perfusion pumps.

[0073] For example, the preset perfusion flow rate V1 of the liquid inlet perfusion pump is set, the preset perfusion flow rate V1 is processed based on the flow rate limitation condition of the perfusion pump to obtain the first target flow rate, and then the liquid inlet perfusion pump is manually started to perfuse at the first target flow rate. The preset perfusion flow rate V2 of the liquid outlet perfusion pump is set, the preset perfusion flow rate V2 is processed based on the flow rate limitation condition of the perfusion pump to obtain the first target flow rate, and then the liquid outlet perfusion pump is manually started to perfuse at the first target flow rate. In this embodiment, the manual mode is added to facilitate user use.

[0074] In some embodiments, the implementation process of the first target flow rate in the associated mode and the manual mode can be the same. Specifically, processing the preset perfusion flow rate based on the flow rate limitation condition of the perfusion pump to obtain the first target flow rate includes the following steps:

[0075] Comparing the preset perfusion flow rate with the flow rate limitation condition of the perfusion pump;

[0076] When the preset perfusion flow rate is greater than the maximum flow rate of the perfusion pump, setting the maximum flow rate of the perfusion pump as the first target flow rate;

[0077] When the preset perfusion flow rate is less than or equal to the maximum flow rate of the perfusion pump, and the preset perfusion flow rate is greater than or equal to the minimum flow rate of the perfusion pump, setting the preset perfusion flow rate as the first target flow rate;

[0078] When the preset perfusion flow rate is less than the minimum flow rate of the perfusion pump, setting the minimum flow rate of the perfusion pump as the first target flow rate, and determining the duty ratio corresponding to the first target flow rate based on the duty ratio flow control mode.

[0079] In this embodiment, the flow rate limitation condition is the maximum flow rate of the perfusion pump, the minimum flow rate of the perfusion pump and the duty ratio control.

[0080] After the preset perfusion flow rate is set, the preset perfusion flow rate is compared with the flow rate limitation condition of the perfusion pump to determine the flow rate; when the preset perfusion flow rate is greater than the maximum flow rate of the perfusion pump, the maximum flow rate of the perfusion pump is set as the first target flow rate due to the mechanical performance limitation of the perfusion pump; at this time, the perfusion pump can be in the maximum open state. When the preset perfusion flow rate is less than or equal to the maximum flow rate of the perfusion pump and greater than or equal to the minimum flow rate of the perfusion pump, the preset perfusion flow rate is set as the first target flow rate; at this time, the perfusion pump can be controlled according to the set parameters. When the preset perfusion flow rate is less than the minimum flow rate of the perfusion pump, the minimum flow rate of the perfusion pump is set as the first target flow rate due to the mechanical performance limitation of the perfusion pump, and the duty cycle corresponding to the first target flow rate is determined based on the duty cycle flow control mode, so that the first target flow rate of the perfusion pump matches the preset perfusion flow rate at this time by combining the duty cycle with the minimum flow rate. In this embodiment, the control flow rate is quickly adjusted to improve the control accuracy.

[0081] wherein the duty cycle = the first target flow rate (the preset perfusion flow rate) / the minimum flow rate of the perfusion pump * the preset control fraction. The duty cycle flow control mode is that the flow control in the preset perfusion flow rate is divided into the preset control fraction, and the ratio of the set flow rate to the minimum flow rate is calculated in the flow rate determination to control the output. Assuming that the preset control fraction is 10 fractions, the preset perfusion flow rate / the minimum flow rate = n, that is, the flow rate controlled by the perfusion pump in 10 fractions of 10 seconds is n*10.

[0082] For example: the total control time is 10 seconds, 10 fractions of 10 seconds is 1 second, and the set perfusion flow rate is 1 ml / s; the minimum flow rate is 2 ml / s. If the output is always according to the minimum flow rate, it is 2*10 = 20 ml; and actually only 1*10 = 10 ml is needed. Therefore, the duty cycle is 1 / 2*10 = 5, which means that the first target flow rate is 2 ml / s, but only 5 s is run, and the remaining 5 s is not run to achieve the same effect.

[0083] In some embodiments, the determination of the first control signal of the perfusion pump according to the liquid weight, the preset perfusion weight and the preset dead zone weight in step S230 comprises the following steps:

[0084] determining a dead zone range according to the preset perfusion weight and the preset dead zone weight; and comparing the liquid weight with the dead zone range;

[0085] generating the opening signal of the perfusion pump for discharging liquid and the opening signal of the perfusion pump for discharging liquid when the liquid weight meets the dead zone range;

[0086] comparing the preset perfusion weight with the liquid weight when the liquid weight does not meet the dead zone range;

[0087] generating an opening signal of the outflow perfusion pump when the preset perfusion weight is greater than the liquid weight;

[0088] generating an opening signal of the inflow perfusion pump when the preset perfusion weight is less than the liquid weight.

[0089] Specifically, the weighing system can collect the liquid weight of the cell culture liquid in real time. The required perfusion weight and dead zone weight of the cell culture liquid are determined according to the process requirements of cell culture; the dead zone range is determined by the preset perfusion weight and the preset dead zone weight; the dead zone range is an interval range value close to the target weight. The dead zone weight is associated with the perfusion weight, for example, the perfusion weight is 50 kg, and the dead zone weight is 1 kg; at this time, the dead zone range is 49 kg (perfusion weight 50 kg - dead zone weight 1 kg) to 50 kg (perfusion weight 50 kg).

[0090] First, compare the liquid weight with the dead zone range, and determine whether to control the direction of perfusion according to the comparison result.

[0091] When the liquid weight meets the dead zone range, the direction of perfusion does not need to be adjusted at this time, and the opening signals of the outflow perfusion pump and the inflow perfusion pump are generated at this time, and the inflow and the outflow are the same to control the liquid weight to be unchanged.

[0092] When the liquid weight does not meet the dead zone range, the direction of perfusion needs to be adjusted at this time, and the preset perfusion weight is compared with the liquid weight, and the inflow perfusion pump is opened according to the comparison result, and new culture liquid is continuously input into the reactor (at this time, the outflow perfusion pump is closed); or the outflow perfusion pump is opened, and the old culture liquid is continuously output to the reactor (at this time, the inflow perfusion pump is closed). Specifically, when the preset perfusion weight is greater than the liquid weight, the opening signal of the outflow perfusion pump is generated (at this time, the inflow perfusion pump is closed); when the preset perfusion weight is less than the liquid weight, the opening signal of the inflow perfusion pump is generated (at this time, the outflow perfusion pump is closed). Repeat the steps of the embodiment until the liquid weight meets the dead zone range, and the opening signals of the outflow perfusion pump and the inflow perfusion pump are generated; achieve the balance of perfusion, perfuse new culture liquid under the preset perfusion weight, and perfuse waste liquid, so as to provide a stable culture environment for cells, reduce the production cost of cell products, and ensure the stable growth of cells at a scientific perfusion rate.

[0093] The embodiment will be described and explained below through preferred embodiments.

[0094] Figure 4 The flow chart of the perfusion control method of the preferred embodiment.

[0095] In the association mode, S51 presets a perfusion flow rate V; S52 when the preset perfusion flow rate V is greater than the maximum flow rate Vmax of the perfusion pump; S53 sets the maximum flow rate Vmax of the perfusion pump as the first target flow rate; S54 when the preset perfusion flow rate V is less than or equal to the maximum flow rate Vmax of the perfusion pump and greater than or equal to the minimum flow rate Vmin of the perfusion pump, S55 takes the preset perfusion flow rate V as the first target flow rate; S56 when the preset perfusion flow rate V is less than the minimum flow rate Vmin of the perfusion pump; S57 sets the minimum flow rate Vmin of the perfusion pump as the first target flow rate and determines the duty cycle corresponding to the first target flow rate based on the duty cycle flow control mode; and S58 determines the final first target flow rate.

[0096] S61 presets a perfusion weight; S71 presets a dead zone weight; S72 when the liquid weight meets the dead zone range; S73 simultaneously generates an opening signal of the perfusion pump for discharging liquid and an opening signal of the perfusion pump for discharging liquid, so that the two perfusion pumps are simultaneously opened. S73 when the liquid weight does not meet the dead zone range; S62 when the preset perfusion weight is greater than the liquid weight; S63 generates an opening signal of the perfusion pump for discharging liquid, so that the perfusion pump for discharging liquid is opened; S64 when the preset perfusion weight is less than the liquid weight; S65 generates an opening signal of the perfusion pump for discharging liquid, so that the perfusion pump for discharging liquid is opened; S66 correspondingly controls the perfusion pump to be opened; and S59 finally realizes perfusion control.

[0097] In the embodiment, the problems of inconvenience in use, failure to realize data tracking and recording, and influence of human factors on perfusion effect are solved, the perfusion system and the weighing system are associated, data tracking and recording can be realized, and the influence of human factors on perfusion effect is reduced; a stable culture environment is provided for cells, the production cost of cell products is reduced, and the stable growth of cells is ensured at a scientific perfusion rate.

[0098] It should be noted that the steps shown in the above flow or the flowchart of the accompanying drawings can be executed in a computer system such as a set of computer executable instructions, and although a logical order is shown in the flowchart, in some cases, the steps shown or described can be executed in an order different from that herein.

[0099] In the embodiment, a perfusion control device is also provided, which is used to realize the above embodiments and preferred embodiments and has been described above. The terms "module", "unit", "sub-unit" and the like used below can be a combination of software and / or hardware that realizes a predetermined function. Although the device described in the following embodiments is preferably realized in software, hardware or a combination of software and hardware is also possible and is conceived.

[0100] Figure 5 is a structure block diagram of the perfusion control device of the embodiment, as Figure 5As shown, the device comprises: a determination module 210, a first entering module 220, a first processing module 230, a second processing module 240, and a first perfusion control module 250; the determination module 210 is configured to determine whether the perfusion system and the weighing system are associated; the first entering module 220 is configured to enter an associated mode when the perfusion system and the weighing system are associated; the first processing module 230 is configured to, in the associated mode, acquire the liquid weight of the cell culture solution through the weighing system; determine the first control signal of the perfusion pump according to the liquid weight, the preset perfusion weight, and the preset dead zone weight; the second processing module 240 is configured to process the preset perfusion flow rate based on the flow rate limitation condition of the perfusion pump to obtain a first target flow rate; and the first perfusion control module 250 is configured to control the perfusion of the cell culture solution according to the first control signal and the first target flow rate.

[0101] Through the above device, the problem that data tracking and recording cannot be realized and the perfusion effect is affected by human factors is solved, the perfusion system and the weighing system are associated, data tracking and recording can be realized, and the influence of human factors on the perfusion effect is reduced.

[0102] In some embodiments, the control device of the perfusion further comprises: a second entering module, a third processing module, and a second perfusion control module; the second entering module is configured to enter a manual mode when the perfusion system and the weighing system are not associated; the third processing module is configured to, in the manual mode, process the preset perfusion flow rate based on the flow rate limitation condition of the perfusion pump to obtain a first target flow rate; and the second perfusion control module is configured to control the perfusion of the target cell according to the second control signal of the manual mode and the first target flow rate.

[0103] In some embodiments, the second processing module 240 or the third processing module is further configured to compare the preset perfusion flow rate with the flow rate limitation condition of the perfusion pump; when the preset perfusion flow rate is greater than the maximum flow rate of the perfusion pump, set the maximum flow rate of the perfusion pump as the first target flow rate; when the preset perfusion flow rate is less than or equal to the maximum flow rate of the perfusion pump and greater than or equal to the minimum flow rate of the perfusion pump, set the preset perfusion flow rate as the first target flow rate; and when the preset perfusion flow rate is less than the minimum flow rate of the perfusion pump, set the minimum flow rate of the perfusion pump as the first target flow rate and determine the duty cycle corresponding to the first target flow rate based on the duty cycle flow control mode.

[0104] In some embodiments, the duty cycle = the first target flow rate / the minimum flow rate of the perfusion pump*the preset control number.

[0105] In some embodiments, the first processing module 230 is further configured to determine a dead zone range according to the preset perfusion weight and a preset dead zone weight, compare the liquid weight with the dead zone range, and generate an opening signal of the perfusion pump for discharging and an opening signal of the perfusion pump for taking in at the same time when the liquid weight meets the dead zone range. When the liquid weight does not meet the dead zone range, compare the preset perfusion weight with the liquid weight, generate the opening signal of the perfusion pump for discharging when the preset perfusion weight is greater than the liquid weight, and generate the opening signal of the perfusion pump for taking in when the preset perfusion weight is less than the liquid weight.

[0106] It should be noted that each of the above modules can be a functional module or a program module, and can be implemented by software or hardware. For the modules implemented by hardware, each of the above modules can be located in the same processor, or each of the above modules can be located in different processors in any combination.

[0107] In this embodiment, a computer device is also provided, which includes a memory and a processor. The memory stores a computer program, and the processor is configured to execute the computer program to perform the steps in any of the above method embodiments.

[0108] Optionally, the computer device can further include a transmission device and an input and output device, wherein the transmission device is connected with the processor, and the input and output device is connected with the processor.

[0109] Optionally, in this embodiment, the processor can be configured to execute the following steps through the computer program:

[0110] S1, determining whether the perfusion system and the weighing system are associated;

[0111] S2, when the perfusion system and the weighing system are associated, entering an association mode;

[0112] S3, in the association mode, obtaining the liquid weight of the cell culture solution through the weighing system, and determining the first control signal of the perfusion pump according to the liquid weight, a preset perfusion weight, and a preset dead zone weight;

[0113] S4, processing the preset perfusion flow rate to obtain a first target flow rate based on a flow rate limitation condition of the perfusion pump;

[0114] S5, controlling the perfusion of the cell culture solution according to the first control signal and the first target flow rate.

[0115] It should be noted that the specific examples in this embodiment can refer to the examples described in the above embodiments and optional implementation manners, which will not be described herein again.

[0116] In addition, in combination with the control method of perfusion provided in the above-mentioned embodiments, a storage medium can also be provided in this embodiment to implement. The storage medium has a computer program stored thereon; the computer program is executed by a processor to implement any one of the control methods of perfusion in the above-mentioned embodiments.

[0117] It should be understood that the specific embodiments described herein are merely exemplary and are not intended to limit the application. Based on the embodiments provided in the present application, all other embodiments obtained by those of ordinary skill in the art without creative efforts fall within the scope of the present application.

[0118] Obviously, the drawings are only some examples or embodiments of the present application, and those of ordinary skill in the art can also apply the present application to other similar situations without creative efforts. In addition, it can be understood that although the work done in the development process may be complex and long, some design, manufacture or production changes made by those of ordinary skill in the art according to the technical content disclosed in the present application are only routine technical means and should not be regarded as insufficient disclosure of the present application.

[0119] The term "embodiment" in the present application means that the specific features, structures or characteristics described in combination with the embodiments can be included in at least one embodiment of the present application. The appearance of this phrase in various places in the specification does not necessarily mean the same embodiment, nor does it mean independence or alternatives to other embodiments. It can be clearly or implicitly understood by those of ordinary skill in the art that the embodiments described in the present application can be combined with other embodiments without conflict.

[0120] The above-described embodiments only express several implementation manners of the present application, and the description is more specific and detailed, but it should not be understood as a limitation on the scope of patent protection. It should be pointed out that for those of ordinary skill in the art, without departing from the concept of the present application, a number of modifications and improvements can be made, which all fall within the scope of the present application. Therefore, the scope of protection of the present application should be subject to the appended claims.

Claims

1. A method for controlling irrigation flow, characterized in that, A control system suitable for irrigation; the irrigation control system includes an irrigation system and a weighing system; the method includes: Determine whether the irrigation system and the weighing system are interconnected; When the irrigation system is associated with the weighing system, it enters the association mode; In the associated mode, the liquid weight of the cell culture medium is obtained through the weighing system; based on the liquid weight, the preset perfusion weight, and the preset dead zone weight, the first control signal of the perfusion pump is determined. Based on the flow rate limitation conditions of the irrigation pump, the preset irrigation flow rate is processed to obtain the first target flow rate, including: The preset irrigation flow rate is compared with the flow rate limit condition of the irrigation pump; When the preset irrigation flow rate is greater than the maximum flow rate of the irrigation pump, the maximum flow rate of the irrigation pump is set as the first target flow rate; When the preset irrigation flow rate is less than or equal to the maximum flow rate of the irrigation pump, and the preset irrigation flow rate is greater than or equal to the minimum flow rate of the irrigation pump, the preset irrigation flow rate is taken as the first target flow rate. When the preset irrigation flow rate is less than the minimum flow rate of the irrigation pump, the minimum flow rate of the irrigation pump is set as the first target flow rate, and the duty cycle corresponding to the first target flow rate is determined based on the duty cycle flow control mode. The duty cycle flow control mode divides the flow control in the preset irrigation flow rate into a preset control number, calculates the ratio of the preset irrigation flow rate to the minimum flow rate in the flow determination, and the duty cycle = first target flow rate / minimum flow rate of the irrigation pump × preset control number, thereby controlling the output. The perfusion of the cell culture medium is controlled according to the first control signal and the first target flow rate.

2. The irrigation control method according to claim 1, characterized in that, The method further includes: When the irrigation system is not associated with the weighing system, enter manual mode; In manual mode, the preset irrigation flow rate is processed based on the flow rate limit condition of the irrigation pump to obtain the first target flow rate; The perfusion of the target cells is controlled based on the second control signal and the first target flow rate in manual mode.

3. The irrigation control method according to claim 1, characterized in that, Based on the liquid weight, the preset perfusion weight, and the preset dead zone weight, the first control signal for the perfusion pump is determined, including: The dead zone range is determined based on the preset perfusion weight and the preset dead zone weight; and the liquid weight is compared with the dead zone range. When the liquid weight meets the dead zone range, both the effluent pump start signal and the influent pump start signal are generated simultaneously.

4. The irrigation control method according to claim 3, characterized in that, The method further includes: When the liquid weight does not meet the dead zone range, the preset perfusion weight is compared with the liquid weight; When the preset perfusion weight is greater than the liquid weight, a start signal for the perfusion pump to discharge liquid is generated; When the preset perfusion weight is less than the liquid weight, a start signal is generated for the perfusion pump to start injecting liquid.

5. A control device for irrigation, characterized in that, include: The module includes a determination module, a first entry module, a first processing module, a second processing module, and a first perfusion control module. The determining module is used to determine whether the irrigation system and the weighing system are associated; The first entry module is used to enter the association mode when the irrigation system is associated with the weighing system; The first processing module is used to obtain the liquid weight of the cell culture medium through the weighing system in the associated mode; and to determine the first control signal of the perfusion pump based on the liquid weight, the preset perfusion weight, and the preset dead zone weight. The second processing module is used to process the preset irrigation flow rate based on the flow rate limitation conditions of the irrigation pump to obtain the first target flow rate; The second processing module is further configured to compare the preset irrigation flow rate with the flow rate limitation condition of the irrigation pump; when the preset irrigation flow rate is greater than the maximum flow rate of the irrigation pump, set the maximum flow rate of the irrigation pump as the first target flow rate; when the preset irrigation flow rate is less than or equal to the maximum flow rate of the irrigation pump, and the preset irrigation flow rate is greater than or equal to the minimum flow rate of the irrigation pump, set the preset irrigation flow rate as the first target flow rate; when the preset irrigation flow rate is less than the minimum flow rate of the irrigation pump, set the minimum flow rate of the irrigation pump as the first target flow rate, and determine the duty cycle corresponding to the first target flow rate based on the duty cycle flow control mode; the duty cycle flow control mode divides the flow control in the preset irrigation flow rate into a preset control portion, calculates the ratio of the preset irrigation flow rate to the minimum flow rate in the flow determination, and the duty cycle = first target flow rate / minimum flow rate of the irrigation pump × preset control portion, thereby controlling the output; The first perfusion control module is used to control the perfusion of cell culture medium according to the first control signal and the first target flow rate.

6. A control system for irrigation, characterized in that, Includes an irrigation system and a weighing system associated with the irrigation system; The weighing system is connected to the reactor and control cabinet in the perfusion system, respectively, and is used to collect the liquid weight of the cell culture medium in the reactor. The irrigation system further includes an inlet pipe, an outlet pipe, an inlet irrigation pump, and an outlet irrigation pump; the inlet pipe connects the reactor and the inlet irrigation pump, and the outlet pipe connects the reactor and the outlet irrigation pump. The control cabinet is connected to the inlet pump and the outlet pump. Steps for performing the irrigation control method according to any one of claims 1 to 4.

7. A computer device, comprising a memory and a processor, characterized in that, The memory stores a computer program, and the processor is configured to run the computer program to perform the steps of the perfusion control method according to any one of claims 1 to 4.

8. A computer-readable storage medium having a computer program stored thereon, characterized in that, When the computer program is executed by the processor, it implements the steps of the irrigation control method according to any one of claims 1 to 4.

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