A microfluidic chip for multi-parameter biochemical molecular detection

Abstract

The present invention provides a microfluidic chip for multi-parameter biochemical molecule detection, which specifically includes a chip body, an injection port provided on the chip body, an injection piping system and a calibration liquid bag located on the front of the chip body, and an electrode assembly area and a liquid derivation system located on the back of the chip body. The injection piping system includes a pretreatment chamber, a pretreatment buffer channel, and a micro-check valve that are sequentially connected to the injection port. The electrode assembly area and the injection piping system are connected via an injection liquid channel embedded in the chip body. The injection liquid channel is connected to the micro-check valve, and at least one calibration liquid channel extends through the micro-check valve and is connected to the calibration liquid bag. A diversion valve is provided between the calibration liquid channel and the calibration liquid bag. The present invention can reduce chip cost and processing difficulty, improve detection speed, sensitivity, and accuracy while ensuring simultaneous detection of multiple parameters, and realize simultaneous detection of multiple indicators in biochemical electrolytes.

Description

Technical Field

[0001] The invention belongs to the field of biosensors and relates to a microfluidic chip for multi-parameter biochemical molecule detection. Background Art

[0002] The latest POCT chemical sensor instruments can test multiple samples simultaneously, significantly reducing testing time and making real-time testing faster. POCT devices are often implemented using biosensors, which immobilize bioenzyme molecules on the solid-phase interface of a microanalytical device. After specifically identifying the analyte, they use electrochemical or optical methods for detection and provide immediate readings. Typical commercial biosensor-based POCT devices include blood glucose meters and blood gas analyzers, which are primarily used for blood glucose monitoring, blood gas analysis, and electrolyte analysis.

[0003] There are currently technologies for biochemical testing consumables that can detect multiple substances at once. Specifically, microelectrodes that detect specific biochemical molecules are integrated into the overall detection device and applied to multi-parameter electrochemical detection. Currently, there are technologies that combine microfluidic systems with POCT products both domestically and internationally. Microfluidics is a technology that controls the operation of microfluidics on a chip with microchannels. Existing POCT-related microfluidic systems still have defects, such as solidification and blockage of the test liquid in the microfluidic channel, easy backflow and miscibility of the test liquid, slow liquid flow rate, low detection efficiency, and waste gas generated by the reaction that affects the detection.

[0004] In view of this, the present invention aims to provide a microfluidic chip for multi-parameter biochemical molecular detection, which integrates the functions of preventing the coagulation and blockage of the test liquid, mixing the calibration liquid, sample liquid detection, waste liquid recovery and waste gas discharge on a single chip, thereby reducing the chip cost and processing difficulty, improving the detection speed, sensitivity and accuracy while ensuring the simultaneous detection of multiple parameters, and realizing the simultaneous detection of multiple indicators in biochemical electrolytes. Summary of the Invention

[0005] In summary, the present invention provides a microfluidic chip for multi-parameter biochemical molecule detection.

[0006] The purpose of the present invention is to provide a microfluidic chip for multi-parameter biochemical molecular detection, which specifically includes a chip body, an injection port arranged on the chip body, an injection pipeline system and a calibration liquid bag located on the front of the chip body, and an electrode assembly area and a liquid derivation system located on the back of the chip body.

[0007] The sampling pipeline system includes a pretreatment chamber, a pretreatment buffer channel and a micro-check valve which are connected to the sampling port in sequence. The electrode assembly area is connected to the sampling pipeline system through an injection liquid channel buried inside the chip body. The injection liquid channel is connected to the micro-check valve, and at least one calibration liquid channel is extended through the micro-check valve to be connected to the calibration liquid bag. A diversion valve is provided between the calibration liquid channel and the calibration liquid bag.

[0008] One end of the electrode assembly area is connected to the sample liquid channel, and the other end is connected to the liquid outlet system through the sample liquid outlet channel buried inside the chip body; the liquid outlet system is connected to the exhaust hole.

[0009] Furthermore, the pretreatment chamber may be filled with immunomagnetic beads, anticoagulant drugs or left idle.

[0010] Furthermore, the pre-treatment buffer channel is a micro circulation channel composed of multiple micro saddle-shaped structures.

[0011] Furthermore, the chip body has a width of 65 to 75 mm and a height of 65 to 75 mm; the width of the injection port pipe is 0.5 to 1.5 mm and the height is 0.5 to 1.5 mm; the width of the injection liquid channel and the calibration liquid channel is 0.5 to 1.5 mm and the height is 0.5 to 1.5 mm; the width of the calibration liquid package is 12.5 to 13.5 mm and the height is 30.5 to 31.5 mm.

[0012] Furthermore, the electrode assembly area is provided with at least one electrode card mounting slot, and one electrode card mounting slot can be loaded with an electrode card. The contact surface of the electrode card and the chip body is provided with an electrode detection site, and the back thereof is provided with a metal site. A sample test slot is provided inside the electrode card mounting slot, and the sample inlet liquid channel passes through all the sample test slots in sequence and is connected to the sample outlet liquid channel. The sample test slot is provided at the location of the electrode detection site; a positioning column is provided in the electrode card mounting slot, and a positioning hole is provided on the surface of the electrode card. The positioning column corresponds to the position of the positioning hole and the size is consistent.

[0013] Furthermore, the sample testing slot is a groove with a fixed volume.

[0014] Furthermore, the width of the sample test slot is 7.5-8.5 mm and the height is 1.0-2.0 mm; the positioning column is cylindrical with a radius of 0.2 mm; the width of the electrode card installation slot is 13.0-14.0 mm and the height is 6.5-7.5 mm.

[0015] Furthermore, the liquid derivation system includes a simple check valve, a waste liquid tank and an exhaust hole.

[0016] Furthermore, the simple check valve structure includes a Tesla valve structure.

[0017] Furthermore, a chip positioning hole is provided on the chip body.

[0018] As attached Figure 1 and attached Figure 2 , Attachment Figure 3 Shown are the structural schematic diagram, front and back schematic diagrams of the microfluidic chip for multi-parameter biochemical molecular detection.

[0019] The inlet allows blood / test samples to be injected into the microfluidic channel via a 1-3 ml syringe. The pretreatment chamber is used for pretreatment of the sample solution, such as separation, filtration, and mixing. Magnetic microspheres can be placed in the pretreatment chamber for molecular extraction of RNA and ctDNA, that is, mixed with the sample for DNA detection. In addition, the pretreatment chamber can also contain other substances, structures, or be left empty. The pretreatment buffer channel has the function of uniform mixing and reducing flow rate, which can improve the pretreatment efficiency of the sample solution and prevent incomplete reaction in the pretreatment chamber. The micro-check valve has a micro-inverted frustum-shaped structure. When the chip is placed horizontally, it is vertically oriented, with the opening larger at the top and smaller at the bottom, preventing sample backflow through flow resistance. The diverter valve can guide the calibration liquid in the correct flow direction, and at the same time, a certain height difference structure prevents the liquid from flowing back into the calibration liquid package. Specifically, the calibration liquid package is higher in the direction and lower in the direction of calibration liquid outflow. The calibration liquid package is used to hold a calibration liquid of standard concentration, and the calibration liquid can be discharged by mechanical or manual squeezing.

[0020] The electrode assembly area is an area dug out on the chip body for sample detection, which is specifically divided into six electrode card mounting slots. The electrode card mounting slots are provided with grooves of fixed volume as sample test slots. At the same time, positioning posts are provided at the two corners. The positions of the sample test slots and the positioning posts correspond to the microelectrode detection sites and positioning holes on the electrode card respectively, so that the electrode card can be installed and fixed in the sample test slot; the sampling liquid channel buried in the chip body passes through the sample test slot position in sequence and is connected to the sampling liquid channel at the outlet. The simple check valve adopts a special structure to prevent the waste liquid tank from flowing back; the waste liquid tank can be loaded with tested liquids, including calibration liquids and test samples. The exhaust hole is used to discharge the gas in the flow channel to facilitate the flow of liquid in the microchannel and has a simple anti-overflow structure.

[0021] The number of electrode card installation slots can be changed according to the number of detection indicators. At the same time, the size of the electrode card installation slots and the size of the electrode card can be changed according to needs. The number of positioning columns can also be changed according to the number and size of the electrode cards.

[0022] All of the above structures are contained inside the chip body and can be completed in one go through injection molding, without the need for additional parts. This greatly simplifies the processing steps of the microfluidic system and realizes the integration of microfluidics and sensor chips.

[0023] As attached Figure 4 The figure shows a schematic diagram of liquid injection in a microfluidic chip for multi-parameter biochemical molecular detection. During sample addition, the microfluidic chip is placed horizontally with the front side facing up. The test liquid is injected from the sample inlet, flows through the pretreatment chamber, flows into the pretreatment buffer channel, and then flows through the micro-check valve. At the same time, a mechanical device is used to squeeze the calibration liquid package, forcing the calibration liquid in the calibration liquid package out. After passing through the diversion valve, it flows into the calibration liquid channel and then passes through the micro-check valve to mix with the test liquid. The mixed liquid passes through the sample test slot and the electrode detection site of the contact electrode card through the sample liquid outlet channel, then flows through the simple check valve and into the waste liquid tank. Gas generated along the way is discharged through the exhaust hole connected to the waste liquid tank, and the detection electrical signal is transmitted through the metal site of the electrode card.

[0024] The beneficial effects of the present invention are:

[0025] (1) The present invention integrates the functions of preventing the solidification and clogging of the test liquid, calibration liquid mixing, sample liquid detection, waste liquid recovery and waste gas discharge on a single chip through microfluidic structure design, thereby reducing chip cost and processing difficulty, improving detection speed, sensitivity and accuracy while ensuring simultaneous detection of multiple parameters, and realizing simultaneous detection of multiple indicators in biochemical electrolytes.

[0026] (2) The microfluidic chip of the present invention is designed with a sample test slot and a fixed measurement volume, making the measurement results more accurate. At the same time, the positioning column design makes it possible to separate the microfluidic chip and the electrode card, simplifying the structure of the microfluidic chip and greatly reducing the difficulty and efficiency of its production process. The electrode card can be installed according to the detection target, making it suitable for blood gas detection. BRIEF DESCRIPTION OF THE DRAWINGS

[0027] The invention is further described with reference to the accompanying drawings, but the embodiments in the accompanying drawings do not constitute any limitation to the invention. A person skilled in the art can obtain other drawings based on the following drawings without inventive effort.

[0028] Figure 1 It is a schematic diagram of the structure of a microfluidic chip for multi-parameter biochemical molecular detection;

[0029] Figure 2 This is a front view schematic diagram of a microfluidic chip for multi-parameter biochemical molecular detection;

[0030] Figure 3 This is a schematic diagram of the back of a microfluidic chip for multi-parameter biochemical molecular detection;

[0031] Figure 4 This is a schematic diagram of liquid sampling on a microfluidic chip for multi-parameter biochemical molecular detection.

[0032] Figure ID:

[0033] 1. Inlet; 2. Pretreatment chamber; 3. Pretreatment buffer channel; 4. Micro check valve; 5. Diversion valve; 6. Calibration liquid bag; 7. Electrode assembly area; 8. Electrode card installation slot; 9. Sample test slot; 10. Positioning column; 11. Simple check valve; 12. Waste liquid tank; 13. Exhaust hole; 14. Positioning hole. DETAILED DESCRIPTION

[0034] In order to make the objectives, technical solutions and advantages of the present invention more clearly understood, the present invention is further described in detail with reference to the following specific embodiments and the accompanying drawings.

[0035] Example 1

[0036] As attached Figure 1 As shown, a microfluidic chip for multi-parameter biochemical molecular detection provided by the present invention specifically includes a chip body, and the chip body has a width of 70 mm, a height of 70 mm, and a thickness of 3.0 mm.

[0037] In this embodiment, the sampling port 1 on the chip body is provided with a sampling pipe therein, which has a width of 1.0 mm and a height of 1.0 mm.

[0038] In this embodiment, the sampling pipeline system and the calibration liquid package 6 are located on the front of the chip body, and the calibration liquid package 6 has a width of 13 mm and a height of 31 mm.

[0039] In this embodiment, the sampling pipeline system includes a pretreatment chamber 2, a pretreatment buffer channel 3 and a micro check valve 4 which are connected to the sampling port in sequence. The electrode assembly area 7 is connected to the sampling pipeline system through an injection liquid channel buried inside the chip body. The injection liquid channel is connected to the micro check valve 4, and a calibration liquid channel is extended through the micro check valve 4 to be connected to the calibration liquid bag 6. A diverter valve 5 is provided between the calibration liquid channel and the calibration liquid bag 6.

[0040] Immunomagnetic beads are placed in the pre-treatment chamber 2.

[0041] The pre-treatment buffer channel 3 is a micro-circulation channel composed of a plurality of micro-saddle-shaped structures.

[0042] The width of the injection liquid channel and the calibration liquid channel is 1.0 mm, and the height is 1.0 mm.

[0043] In this embodiment, the electrode assembly area 7 and the liquid derivation system are located on the back of the chip body.

[0044] In this embodiment, one end of the electrode assembly area 7 is connected to the sample liquid channel, and the other end is connected to the liquid outlet system through the sample liquid outlet channel buried inside the chip body; the liquid outlet system is connected to the exhaust hole 13.

[0045] In this embodiment, the electrode assembly area 7 is provided with two rows of six electrode card mounting slots 8. One electrode card mounting slot 8 can be loaded with one electrode card. The contact surface of the electrode card and the chip body is provided with an electrode detection site, and a metal site is provided on the back thereof. A sample test slot 9 is provided inside the electrode card mounting slot 8. The sample inlet liquid channel passes through all the sample test slots 9 in sequence and is connected to the sample outlet liquid channel. The sample test slot 9 is provided at the location of the electrode detection site; a positioning column 10 is provided in the electrode card mounting slot 8, and a positioning hole is provided on the surface of the electrode card. The positioning column 10 corresponds to the position of the positioning hole and has the same size.

[0046] The sample test slot 9 is a fixed-volume groove with a width of 8.1 mm and a height of 1.5 mm. The positioning column is cylindrical with a radius of 0.2 mm. The electrode card installation slot 8 is 13.4 mm wide and 7.0 mm high.

[0047] In this embodiment, the liquid derivation system includes a simple check valve 11 , a waste liquid tank 12 and an exhaust hole 13 .

[0048] The structure of the simple check valve 11 is a Tesla valve structure.

[0049] In this embodiment, a chip positioning hole is further provided on the chip body, and the hole diameter is 1.0 mm.

[0050] Example 2

[0051] This embodiment provides a microfluidic chip for multi-parameter biochemical molecular detection, which specifically includes a chip body. The chip body has a width of 70 mm, a height of 70 mm, and a thickness of 3.0 mm.

[0052] In this embodiment, the sampling port 1 on the chip body is provided with a sampling pipe therein, which has a width of 1.5 mm and a height of 1.5 mm.

[0053] In this embodiment, the sampling pipeline system and the calibration liquid package 6 are located on the front of the chip body, and the calibration liquid package 6 has a width of 12 mm and a height of 30 mm.

[0054] In this embodiment, the sampling pipeline system includes a pretreatment chamber 2, a pretreatment buffer channel 3 and a micro check valve 4 which are connected to the sampling port in sequence. The electrode assembly area 7 is connected to the sampling pipeline system through an injection liquid channel buried inside the chip body. The injection liquid channel is connected to the micro check valve 4, and a calibration liquid channel is extended through the micro check valve 4 to be connected to the calibration liquid bag 6. A diverter valve 5 is provided between the calibration liquid channel and the calibration liquid bag 6.

[0055] Anticoagulant drugs are placed in the pre-treatment chamber 2 .

[0056] The pre-treatment buffer channel 3 is a micro-circulation channel composed of a plurality of micro-saddle-shaped structures.

[0057] The width of the injection liquid channel and the calibration liquid channel is 1.0 mm, and the height is 1.0 mm.

[0058] In this embodiment, the electrode assembly area 7 and the liquid derivation system are located on the back of the chip body.

[0059] In this embodiment, one end of the electrode assembly area 7 is connected to the sample liquid channel, and the other end is connected to the liquid outlet system through the sample liquid outlet channel buried inside the chip body; the liquid outlet system is connected to the exhaust hole 13.

[0060] In this embodiment, the electrode assembly area 7 is provided with two rows of four electrode card mounting slots 8. One electrode card mounting slot 8 can be loaded with one electrode card. The contact surface of the electrode card and the chip body is provided with an electrode detection site, and a metal site is provided on the back thereof. A sample test slot 9 is provided inside the electrode card mounting slot 8. The sample inlet liquid channel passes through all the sample test slots 9 in sequence and is connected to the sample outlet liquid channel. The sample test slot 9 is provided at the location of the electrode detection site; a positioning column 10 is provided in the electrode card mounting slot 8, and a positioning hole is provided on the surface of the electrode card. The positioning column 10 corresponds to the position of the positioning hole and the size is consistent.

[0061] The sample test slot 9 is a fixed-volume groove with a width of 8.0 mm and a height of 1.5 mm. The positioning column is cylindrical with a radius of 0.2 mm. The electrode card installation slot 8 is 14.5 mm wide and 7.5 mm high.

[0062] In this embodiment, the liquid derivation system includes a simple check valve 11 , a waste liquid tank 12 and an exhaust hole 13 .

[0063] The structure of the simple check valve 11 is a Tesla valve structure.

[0064] In this embodiment, a chip positioning hole is further provided on the chip body, and the hole diameter is 1.0 mm.

[0065] Example 3

[0066] This embodiment provides a microfluidic chip for multi-parameter biochemical molecular detection, which specifically includes a chip body. The chip body has a width of 70 mm, a height of 70 mm, and a thickness of 3.0 mm.

[0067] In this embodiment, the sampling port 1 on the chip body is provided with a sampling pipe therein, which has a width of 1.5 mm and a height of 1.5 mm.

[0068] In this embodiment, the sampling pipeline system and the calibration liquid package 6 are located on the front of the chip body, and the calibration liquid package 6 has a width of 12 mm and a height of 30 mm.

[0069] In this embodiment, the sampling pipeline system includes a pretreatment chamber 2, a pretreatment buffer channel 3 and a micro check valve 4 which are connected to the sampling port in sequence. The electrode assembly area 7 is connected to the sampling pipeline system through an injection liquid channel buried inside the chip body. The injection liquid channel is connected to the micro check valve 4, and a calibration liquid channel is extended through the micro check valve 4 to be connected to the calibration liquid bag 6. A diverter valve 5 is provided between the calibration liquid channel and the calibration liquid bag 6.

[0070] The pre-treatment chamber 2 is empty.

[0071] The pre-treatment buffer channel 3 is a micro-circulation channel composed of a plurality of micro-saddle-shaped structures.

[0072] The width of the injection liquid channel and the calibration liquid channel is 1.5 mm, and the height is 1.5 mm.

[0073] In this embodiment, the electrode assembly area 7 and the liquid derivation system are located on the back of the chip body.

[0074] In this embodiment, one end of the electrode assembly area 7 is connected to the sample liquid channel, and the other end is connected to the liquid outlet system through the sample liquid outlet channel buried inside the chip body; the liquid outlet system is connected to the exhaust hole 13.

[0075] In this embodiment, the electrode assembly area 7 is provided with a row of three electrode card mounting slots 8. One electrode card mounting slot 8 can be loaded with one electrode card. The contact surface of the electrode card and the chip body is provided with an electrode detection site, and a metal site is provided on the back thereof. A sample test slot 9 is provided inside the electrode card mounting slot 8. The sample inlet liquid channel passes through all the sample test slots 9 in sequence and is connected to the sample outlet liquid channel. The sample test slot 9 is provided at the location of the electrode detection site; a positioning column 10 is provided in the electrode card mounting slot 8, and a positioning hole is provided on the surface of the electrode card. The positioning column 10 corresponds to the position of the positioning hole and has the same size.

[0076] The sample test slot 9 is a fixed-volume groove with a width of 8.0 mm and a height of 1.5 mm. The positioning column is cylindrical with a radius of 0.2 mm. The electrode card installation slot 8 is 13.0 mm wide and 6.5 mm high.

[0077] In this embodiment, the liquid derivation system includes a simple check valve 11 , a waste liquid tank 12 and an exhaust hole 13 .

[0078] The structure of the simple check valve 11 is a Tesla valve structure.

[0079] In this embodiment, a chip positioning hole is further provided on the chip body, and the hole diameter is 1.0 mm.

[0080] Example 4

[0081] According to the multi-parameter biochemical molecular detection microfluidic chip provided in Example 1, Figure 4 , which is a schematic diagram of liquid sampling in a microfluidic chip for multi-parameter biochemical molecular detection.

[0082] When adding samples, the microfluidic chip is placed horizontally with the front side facing up. The liquid to be tested is injected from the sampling port 1, flows through the pretreatment chamber 2, flows into the pretreatment buffer channel 3, and then flows through the micro check valve 4. At the same time, a mechanical device is used to squeeze the calibration liquid package 6, and the calibration liquid in the calibration liquid package 6 is squeezed out. After passing through the guide valve 5, it flows into the calibration liquid channel, and then passes through the micro check valve 4 to mix with the liquid to be tested; the mixed liquid passes through the sample test slot 9 and the electrode detection site of the contact electrode card in sequence through the sampling liquid channel, and then passes through the sample outlet liquid channel, flows through the simple check valve 11, and enters the waste liquid tank 12. The gas generated on the way is discharged through the exhaust hole 13 connected to the waste liquid tank 12, and the detection electrical signal is transmitted through the metal site of the electrode card.

[0083] It will be apparent to those skilled in the art that the present invention is not limited to the details of the exemplary embodiments described above and that the invention can be embodied in other specific forms without departing from the spirit or essential characteristics of the invention. Therefore, the embodiments should be considered in all respects as illustrative and non-restrictive, and the scope of the invention is defined by the appended claims rather than the foregoing description, and it is intended that all variations that come within the meaning and range of equivalents of the claims be embraced therein.

[0084] In addition, it should be understood that although this specification is described in terms of implementation methods, not every implementation method contains only one independent technical solution. This description is for clarity only. Those skilled in the art should consider this specification as a whole. The technical solutions in each embodiment can also be appropriately combined to form other implementation methods that can be understood by those skilled in the art. Technical details not described in detail in this invention can be implemented by any existing technology in the art. In particular, all technical features not described in detail in this invention can be implemented by any existing technology.

Claims

1. A microfluidic chip for multi-parameter biochemical molecular detection, characterized in that: It includes a chip body, a The chip body comprises an injection port (1), an injection pipe system and a calibration liquid bag (6) located on the front of the chip body, and an electrode assembly area (7) and a liquid derivation system located on the back of the chip body; the injection pipe system comprises a pretreatment chamber (2), a pretreatment buffer channel (3) and a micro check valve (4) which are sequentially connected to the injection port; the electrode assembly area (7) is connected to the injection pipe system via an injection liquid channel buried inside the chip body; the injection liquid channel is connected to the micro check valve (4), and at least one calibration liquid channel is extended through the micro check valve (4) and connected to the calibration liquid bag (6); a diversion valve (5) is provided between the calibration liquid channel and the calibration liquid bag (6); one end of the electrode assembly area (7) is connected to the injection liquid channel, and the other end is connected to the liquid derivation system via a sample liquid outlet channel buried inside the chip body; the liquid derivation system is connected to an exhaust hole (13); The electrode assembly area (7) is provided with at least one electrode card installation slot (8), and one electrode card installation slot (8) can be loaded with one electrode card. The contact surface of the electrode card with the chip body is provided with an electrode detection site, and the back surface thereof is provided with a metal site. A sample test slot (9) is provided inside the electrode card installation slot, and the sample inlet liquid channel passes through all the sample test slots (9) in sequence and is connected to the sample outlet liquid channel. The sample test slot (9) is provided at the location of the electrode detection site; a positioning column (10) is provided inside the electrode card installation slot (8), and a positioning hole is provided on the surface of the electrode card. The positioning column (10) corresponds to the position of the positioning hole and has the same size as the positioning hole; The pre-treatment chamber (2) can be filled with immunomagnetic beads, anticoagulant drugs or left idle.

2. A microfluidic chip for multi-parameter biochemical molecular detection according to claim 1, characterized in that: The pre-treatment buffer channel (3) is a micro-circulation channel composed of a plurality of micro-saddle-shaped structures.

3. A microfluidic chip for multi-parameter biochemical molecular detection according to claim 1, characterized in that: The chip body has a width of 65 to 75 mm and a height of 65 to 75 mm; the injection port (1) pipe has a width of 0.5 to 1.5 mm and a height of 0.5 to 1.5 mm; the injection liquid channel and the calibration liquid channel have a width of 0.5 to 1.5 mm and a height of 0.5 to 1.5 mm; the calibration liquid bag (6) has a width of 12.5 to 13.5 mm and a height of 30.5 to 31.5 mm.

4. A microfluidic chip for multi-parameter biochemical molecular detection according to claim 1, characterized in that: The sample testing slot (9) is a groove with a fixed volume.

5. The microfluidic chip for multi-parameter biochemical molecular detection according to claim 1, characterized in that: The sample test slot (9) has a width of 7.5-8.5 mm and a height of 1.0-2.0 mm; the positioning column (10) is cylindrical with a bottom radius of 0.2 mm; the electrode card installation slot (8) has a width of 13.0-14.0 mm and a height of 6.5-7.5 mm.

6. The microfluidic chip for multi-parameter biochemical molecular detection according to claim 1, characterized in that: The liquid derivation system comprises a simple check valve (11), a waste liquid tank (12) and an exhaust hole (13).

7. A microfluidic chip for multi-parameter biochemical molecular detection according to claim 6, characterized in that: The simple check valve (11) structure includes a Tesla valve structure.

8. The microfluidic chip for multi-parameter biochemical molecular detection according to claim 1, characterized in that: A chip positioning hole (14) is also provided on the chip body.

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