Active compositions, cosmetic compositions and uses thereof, repair serums and methods of making same

Abstract

The present application relates to the field of cosmetics, and discloses an active composition for skin anti-inflammatory, soothing and repairing, a cosmetic composition and application thereof, a repair essence and a preparation method thereof. The active composition contains a prophylactic effective amount of two or more of the following components mixed or stored independently: glycerophosphoinositol choline salt, essential oil liposome, glucosaminoglycan, lactic acid bacteria turmeric ferment, mannose erythritol lipid, ergothioneine, seaweed oligosaccharide, hyaluronic acid, glycerol glucoside and ectoine. The active composition can regulate the skin micro-ecology from the root, improve the skin microcirculation, repair the skin barrier, and has excellent anti-inflammatory, soothing and repairing effects.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of cosmetics, in particular to an active composition for skin anti-inflammatory, soothing and repairing, a cosmetic composition and its application, a repair essence and a preparation method thereof. BACKGROUND

[0002] Skin is the largest organ covering the surface of the human body. Skin is directly in contact with the external environment and is easily damaged by excessive sunlight, environmental humidity, temperature, dust and PM2.5 in the air and other factors. At the same time, with the improvement of living standards and consumption upgrading, the public is increasingly demanding of beauty. In this information society, people blindly follow the trend and blindly pursue high-concentration high-activity skincare products, coupled with repeated over-care and over-exfoliation, leading to a sharp increase in the number of sensitive skin in society in just a few decades. In addition, with the development and internalization of society, most people are always in a state of anxiety and high pressure, accompanied by late sleep, overeating sweets, trans fats, spicy food. People in a state of high pressure and sub-health for a long time have very low immunity and are very prone to skin problems, leading to increasingly fragile and sensitive skin. Such a large number of sensitive and weak skin people are in urgent need of finding a truly suitable skincare product to restore their skin to a strong, healthy and vibrant state.

[0003] However, most of the currently marketed skincare products for sensitive and weak skin have the following problem: high activity is often accompanied by high irritation, high instability and sensitivity; while very mild and skin-friendly skincare products often have low activity, and only macromolecular colloid or non-polar mineral oil, squalane, silicone oil, etc. are used to achieve a packaging effect, the purpose of which is to eliminate any irritation and create a moisturizing environment for the skin to allow the skin to recover slowly. But these two ways are inefficient and unsatisfactory for sensitive and weak skin care.

[0004] CN112603867A discloses a skin film repair essence liquid and a preparation method thereof. The skin film repair essence liquid comprises water, a chelating agent, a moisturizing agent, glycerophosphoinositol choline salt, tetrahydropyrimidine, stephania tetrandra extract, a solvent, D-panthenol, gentian extract, ceramide-2, a preservative synergist, etc. The skin film repair essence liquid has excellent anti-allergic, anti-inflammatory and anti-itching effects through the cooperation of glycerophosphoinositol choline salt, tetrahydropyrimidine and stephania tetrandra extract. The addition of gentian extract can inhibit irritation, improve anti-allergic and anti-inflammatory effects, barrier repair function and oil control effect, and provide ceramide-2 to firmly maintain the required moisture of the skin, significantly repair the stratum corneum, and relieve the sensitive symptoms of hormone-dependent skin. However, the active substances used in the essence liquid are almost water-soluble, and the core active substances glycerophosphoinositol choline salt and tetrahydropyrimidine carboxylic acid are ionic active substances after water dissolution, which are difficult to smoothly pass through the stratum corneum containing lipids and the double-layer charge layer of the skin, resulting in low transdermal absorption rate and poor anti-inflammatory effect.

[0005] CN113679628A discloses a comprehensive skin problem improvement essence liquid. The essence liquid comprises a skin care composition for comprehensive improvement of skin problems 0.08-10%, a moisturizing agent 5-50%, a thickening agent 0.05-0.5%, and water 40-99%. The skin care composition comprises ikedoid, ergothioneine, boswelia, glutathione and acetyl hexapeptide-8. The moisturizing agent comprises any one or a combination of at least two of polyhydric alcohols, sodium hyaluronate, trehalose, tremella polysaccharide, betaine, beta-glucan, squalane, algal extract, ceramide or collagen. The essence liquid has strong comprehensive repair effect on skin problems, including moisturizing, whitening, antioxidant, skin tightening and skin lightening. However, the essence liquid also has the problem of low transdermal absorption rate. Moreover, the active combination of the essence liquid pursues comprehensiveness, which is not precise enough in targeting, and it is difficult to truly solve the problems of sensitive and inflammatory skin.

[0006] Therefore, there is an urgent need to develop an active composition and a repair essence that truly have high-efficiency anti-inflammatory, soothing and repair effects, realize precise formula and scientific active combination, can regulate the skin micro-ecology from the root, improve the microcirculation of the skin, repair the skin barrier, have excellent anti-inflammatory, soothing and antioxidant effects, and truly realize high-efficiency transdermal absorption in an extremely mild and skin-friendly manner. SUMMARY

[0007] The purpose of the present application is to overcome the problem of poor anti-inflammatory and soothing effects of existing cosmetics for skin moisturizing.

[0008] To achieve the above-mentioned purpose, the first aspect of the present application provides an active composition for skin anti-inflammatory, soothing and repair, which comprises a prophylactically effective amount of two or more of the following components mixed and stored or stored independently:

[0009] glycerophosphoinositol choline salt, essential oil liposome, glucosaminoglucan, lactic acid bacteria turmeric ferment, mannose erythritol lipid, ergothioneine, seaweed oligosaccharide, hyaluronic acid, glycerol glucoside, ectoine;

[0010] In the active composition, the content of the essential oil liposome is 80-500 parts by weight, the content of the glucosaminoglucan is 30-70 parts by weight, the content of the lactic acid bacteria turmeric ferment is 100-320 parts by weight, the content of the mannose erythritol lipid is 20-50 parts by weight, the content of the ergothioneine is 0.1-10 parts by weight, the content of the seaweed oligosaccharide is 10-80 parts by weight, the content of the hyaluronic acid is 5-50 parts by weight, the content of the glycerol glucoside is 20-325 parts by weight, and the content of the ectoine is 10-260 parts by weight, relative to 100 parts by weight of the glycerophosphoinositol choline salt.

[0011] The second aspect of the present application provides a cosmetic composition for skin, characterized in that the composition is composed of an active ingredient and an auxiliary material, the active ingredient is the active composition described in the first aspect, and the total content of the active ingredient is 5.0% by weight or more.

[0012] The third aspect of the present application provides the use of the cosmetic composition for skin described in the second aspect in essence.

[0013] The fourth aspect of the present application provides a repair essence, which is composed of A phase, B phase, C phase and D phase, the A phase contains glycerol, asiaticoside, beta-glucan, rose hydrolat, magnolia hydrolat, panthenol, inositol, allantoin, L-rhamnose;

[0014] The B phase contains glycerophosphoinositol choline salt, glucosaminoglucan, lactic acid bacteria turmeric ferment, mannose erythritol lipid, ergothioneine, seaweed oligosaccharide, hyaluronic acid, glycerol glucoside, ectoine;

[0015] The C phase contains Ultra Care RH23; the Ultra Care RH23 is Ultra Care RH23 produced by AMT BIOTECH LTD company;

[0016] The D phase contains 1,3-butanediol and essential oil liposome.

[0017] In the serum, the content of the glycerol is 100-500 parts by weight, the content of the asiaticoside is 10-70 parts by weight, the content of the beta-glucan is 1-30 parts by weight, the content of the rose hydrolat is 1000-7600 parts by weight, the content of the michelia hydrolat is 500-6500 parts by weight, the content of the panthenol is 100-300 parts by weight, the content of the inositol is 10-100 parts by weight, the content of the allantoin is 10-25 parts by weight, and the content of the L-rhamnose is 20-150 parts by weight, relative to 100 parts by weight of the glycerophosphoinositol choline salt;

[0018] The content of the glucosaminoglycan is 30-70 parts by weight, the content of the lactic acid bacteria turmeric ferment is 100-320 parts by weight, the content of the mannose erythritol lipid is 20-50 parts by weight, the content of the ergothioneine is 0.1-10 parts by weight, the content of the seaweed oligosaccharide is 10-80 parts by weight, the content of the hyaluronic acid is 5-50 parts by weight, the content of the glycerol glucoside is 20-325 parts by weight, and the content of the ekdunin is 10-260 parts by weight;

[0019] The content of the Ultra Care RH23 is 80-120 parts by weight;

[0020] The content of the essential oil liposome is 80-500 parts by weight, and the content of the 1,3-butanediol is 100-300 parts by weight.

[0021] The fifth aspect of the present application provides a method for preparing the repairing serum of the fourth aspect, characterized in that the method comprises:

[0022] (1) first mixing the components in phase A to obtain a mixed material I;

[0023] (2) second mixing phase B with the mixed material I to obtain a mixed material II;

[0024] (3) third mixing phase C with the mixed material II to obtain a mixed material III;

[0025] (4) fourth mixing phase D with the mixed material III to obtain the repairing serum.

[0026] The composition provided by the present application utilizes glycerophosphoinositol choline salt, essential oil liposome, glucosaminoglucan, lactic acid bacteria turmeric ferment, mannose erythritol lipid, ergothioneine, seaweed oligosaccharide, hyaluronic acid, glycerol glucoside, and ectoine in specific contents for synergistic combination, so as to obtain an active composition with excellent anti-inflammatory, soothing, and repairing effects. The addition of glycerol glucoside, ectoine, and hyaluronic acid in the active composition is beneficial to realize long-acting deep moisturizing; the essential oil liposome and glycerophosphoinositol choline GPI, lactic acid bacteria turmeric ferment, and glycerol glucoside can inhibit the source of inflammatory cascade reaction from the root, and comprehensively inhibit various pro-inflammatory factors from multiple dimensions, so that the active composition has excellent soothing, repairing, and maintaining skin barrier functions. Meanwhile, ergothioneine and seaweed oligosaccharide can provide persistent and steady-state multi-antioxidation, scavenge superoxide anion, hydrogen peroxide, and hydroxyl radical, prevent cell membrane lipid peroxidation, and stimulate the natural antioxidant defense system of cells; glucosaminoglucan, seaweed oligosaccharide, and mannose erythritol lipid can not only strongly promote the transdermal absorption of the above-mentioned active substances with anti-inflammatory, soothing, antioxidant, and long-acting moisturizing effects, but also promote the microcirculation of the skin itself, and further strengthen the supply of nutrients to the skin itself.

[0027] The active composition is used in cosmetics, in particular, serum, can regulate the skin microecology from the root, improve the microcirculation of the skin, repair the skin barrier, and provide a mild and efficient repairing liquid serum for sensitive and weak skin, so as to achieve the effects of anti-inflammatory, soothing, and repairing. BRIEF DESCRIPTION OF DRAWINGS

[0028] Figure 1 is a comparison chart of the red area of the face of a subject before and after using the serum of Example 2 for 14 days;

[0029] Figure 2 is a comparison chart of the red area of the face of a subject before and after using the serum of Example 4 for 14 days;

[0030] Figure 3 is a comparison chart of the red area of the face of a subject before and after using the serum of Example 8 for 14 days. DETAILED DESCRIPTION

[0031] The endpoints of the ranges and any values disclosed herein are not limited to the precise values stated. The endpoints of the ranges and the values stated are approximations that are adopted to more readily describe some embodiments. It is to be understood that the endpoints of both ranges and values stated are not to be construed as limiting. Within this context, it is to be understood that the use of a term for one prior-art document in the description or claims to describe a feature is not to be construed as an admission that the feature is considered to be prior art to that document, unless specifically stated as such. Ranges can be expressed herein with endpoints that are not absolute. For example, a range of "about 1 to 5" can be expressed as "about 1 to about 5" or "between about 1 and about 5." Unless otherwise indicated, all ranges include all the sub-ranges of the same entire range, all identified decimal numbers between the endpoints are specifically contemplated, and all values or sub-ranges between the endpoints are specifically contemplated. It is specifically contemplated that endpoints can be combined to form new ranges not expressly disclosed. For example, the range "about 1 to about 5" is specifically contemplated as a new range even though it is not expressly disclosed.

[0032] It should be noted that in the aspects of the present application, for the same components in the aspects, the present application is described only once in one of the aspects without repeated description, and the person skilled in the art should not understand it as a limitation of the present application.

[0033] It should be noted that in the present application, the raw materials not mentioned can be purchased by the person skilled in the art according to the needs, and will not be described one by one in the present application.

[0034] As described before, the first aspect of the present application provides an active composition for skin anti-inflammatory, soothing and repairing, which contains a prophylactically effective amount of two or more of the following components mixed or stored independently:

[0035] Glycerophosphoinositol choline salt, essential oil liposome, glucosaminoglucan, lactic acid bacteria turmeric ferment, mannose erythritol lipid, ergothioneine, seaweed oligosaccharide, hyaluronic acid, glycerol glucoside, ectoine;

[0036] In the active composition, the content of the essential oil liposome is 80-500 parts by weight, the content of the glucosaminoglucan is 30-70 parts by weight, the content of the lactic acid bacteria turmeric ferment is 100-320 parts by weight, the content of the mannose erythritol lipid is 20-50 parts by weight, the content of the ergothioneine is 0.1-10 parts by weight, the content of the seaweed oligosaccharide is 10-80 parts by weight, the content of the hyaluronic acid is 5-50 parts by weight, the content of the glycerol glucoside is 20-325 parts by weight, and the content of the ectoine is 10-260 parts by weight, relative to 100 parts by weight of the glycerophosphoinositol choline salt.

[0037] Preferably, in the active composition, the content of the essential oil liposome is 100-400 parts by weight, the content of the glucosaminoglucan is 40-60 parts by weight, the content of the lactic acid bacteria turmeric ferment is 150-310 parts by weight, the content of the mannose erythritol lipid is 20-40 parts by weight, the content of the ergothioneine is 2-8 parts by weight, the content of the seaweed oligosaccharide is 20-60 parts by weight, the content of the hyaluronic acid is 8-45 parts by weight, the content of the glycerol glucoside is 50-250 parts by weight, and the content of the ectoine is 20-220 parts by weight, relative to 100 parts by weight of the glycerophosphoinositol choline salt. The inventors found in the research process that the composition provided in this preferred case has better effect for skin anti-inflammatory, soothing and repairing.

[0038] The active composition of the present application contains the glycerophosphoinositol choline GPI in a specific amount, which acts on the source of inflammatory cascade, inhibits the release of cytosolic phospholipase A2 and IL-1a in keratinocytes, and relieves the erythema caused by UVB, so that the scheme of the present application can have the effect of anti-inflammatory soothing.

[0039] Preferably, the glycerophosphoinositol choline is glycerophosphoinositol choline GPICHOLINE PE purchased from SEDERMA S.A.S. company.

[0040] The specific amount of the essential oil liposome contained in the active composition of the present application enables the scheme of the present application to have the effect of repairing and soothing.

[0041] Preferably, the essential oil liposome is prepared by emulsifying and encapsulating Roman chamomile essential oil, German chamomile essential oil, everlasting flower essential oil and chastetree essential oil in phospholipids to form nanoliposomes by a high-pressure microfluidizer homogenizer.

[0042] Preferably, the essential oil liposome contains 2-5% by weight of Roman chamomile essential oil, 2-5% by weight of German chamomile essential oil, 1-5% by weight of Italian everlasting flower essential oil and 0.5-2% by weight of chastetree essential oil, based on the total weight of the essential oil liposome. The inventors found that the active composition obtained in this preferred case has more excellent anti-inflammatory and soothing effects.

[0043] Preferably, in order to further improve the anti-inflammatory and soothing effects of the active composition, the inventors provide a preferred method for preparing the essential oil liposome, which comprises the following steps:

[0044] The phospholipid, stearic acid and butanediol are uniformly stirred at 75-85°C until completely dissolved, and then the Roman chamomile essential oil, German chamomile essential oil, Italian everlasting flower essential oil and chastetree essential oil are added and continue to be stirred until uniformly mixed to obtain an oil phase mixture. The temperature of the oil phase mixture is maintained at 70-80°C, and then water at 70-80°C is added to the oil phase mixture, which is homogenized at a speed of 9000-11000 rpm under a high-speed homogenizer for 3-7 min to obtain an essential oil phospholipid emulsion; the essential oil phospholipid emulsion is injected into a microfluidizer homogenizer, and is subjected to shear treatment at a pressure of 15-20 thousand PSI for 10-15 seconds, and the discharge temperature is controlled at 25-35°C by a heat exchanger to obtain the essential oil liposome.

[0045] The phospholipid is used in an amount of 18-25 parts by weight, the stearic acid is used in an amount of 0.5-1.5 parts by weight, the butanediol is used in an amount of 0.1-1.5 parts by weight, the Roman chamomile essential oil is used in an amount of 2-5 parts by weight, the German chamomile essential oil is used in an amount of 2-5 parts by weight, the Italian everlasting flower essential oil is used in an amount of 1-5 parts by weight, the Myroxylon balsamum essential oil is used in an amount of 0.5-2 parts by weight, and the balance is water, based on 100 parts by weight of the total weight of the essential oil liposome.

[0046] The active composition of the present application contains the Curcuma longa fermented product of lactic acid bacteria in a specific amount, so that the scheme of the present application can have the effects of moisturizing and anti-oxidation, and can instantaneously relieve the stinging, burning, redness, itching and other symptoms of sensitive skin, and achieve the effects of anti-inflammation, soothing, and relieving itching.

[0047] Preferably, the Curcuma longa fermented product of lactic acid bacteria is a product obtained by fermenting Curcuma longa with a lactic acid bacteria population; and the lactic acid bacteria are selected from at least one of Lactobacillus rhamnosus, Lactobacillus acidophilus, Lactobacillus plantarum, and Lactobacillus reuteri. More preferably, the lactic acid bacteria are Lactobacillus rhamnosus.

[0048] In a particularly preferred case, the Curcuma longa fermented product of lactic acid bacteria is Turmeric gel (Curcuma longa fermented product of lactic acid bacteria) produced by the Korean company Sajo Industries Co., Ltd. The inventors have found that the active composition obtained in this preferred case has more excellent effects of anti-inflammation and soothing.

[0049] The active composition of the present application contains the seaweed oligosaccharide in a specific amount, so that the scheme of the present application can have the effect of anti-oxidation, improve the activities of superoxide dismutase SOD and glutathione peroxidase GSH-PX, help keratinocytes against oxidative stress damage of UVB, and have excellent thermal stability and system stability.

[0050] Preferably, the seaweed oligosaccharide is a low-molecular high-activity algal oligosaccharide formed by connecting multiple monosaccharide groups through glycosidic bonds.

[0051] In a preferred case, the seaweed oligosaccharide is a multi-component oligosaccharide mixture obtained by directional extraction from at least one marine alga selected from the group consisting of red algae, Antarctic ice algae, and polar brown algae. More preferably, the seaweed oligosaccharide is Antarctic ice algae oligosaccharide.

[0052] In a preferred case, the molecular weight of the seaweed oligosaccharide is below 10,000 daltons; further preferably, the oligosaccharide has a molecular weight of 4,000-6,000 daltons.

[0053] The active composition of the present application contains a specific amount of the glyceryl glucoside, which can reduce the expression of interleukin 1 alpha and interleukin 1 beta, inhibit mast cell degranulation, and thus improve the cell healing ability of the present application, and further improve the anti-inflammatory and soothing ability of the present application.

[0054] Preferably, the glyceryl glucoside is 2-alpha configuration glyceryl glucoside produced by Qingdao Zhongke Lan Zhi Biological Technology Development Co., Ltd. The inventors have found that in this preferred case, the active composition obtained has better moisturizing, anti-inflammatory and soothing ability.

[0055] The active composition of the present application contains a specific amount of the glyceryl glucoside, which can reduce the expression of interleukin 1 alpha and interleukin 1 beta, inhibit mast cell degranulation, and thus improve the cell healing ability of the present application, and further improve the anti-inflammatory and soothing ability of the present application.

[0056] Preferably, the molecular weight of the glyceryl glucoside is 40,000 to 60,000 daltons.

[0057] More preferably, the glyceryl glucoside is selected from the glyceryl glucoside produced by Shanghai Huiwen Biological Technology Co., Ltd. The inventors have found that in this preferred case, the active composition obtained is more stable and has better repair effect.

[0058] The active composition of the present application contains a specific amount of the glyceryl glucoside, which can reduce the expression of interleukin 1 alpha and interleukin 1 beta, inhibit mast cell degranulation, and thus improve the cell healing ability of the present application, and further improve the anti-inflammatory and soothing ability of the present application.

[0059] Preferably, the hyaluronic acid is full-spectrum hyaluronic acid.

[0060] Preferably, the hyaluronic acid is low molecular weight hyaluronic acid with a molecular weight of less than 20,000 daltons.

[0061] The active composition of the present application contains a specific amount of the glyceryl glucoside, which can reduce the expression of interleukin 1 alpha and interleukin 1 beta, inhibit mast cell degranulation, and thus improve the cell healing ability of the present application, and further improve the anti-inflammatory and soothing ability of the present application.

[0062] The active composition of the present application contains a specific amount of the glyceryl glucoside, which can reduce the expression of interleukin 1 alpha and interleukin 1 beta, inhibit mast cell degranulation, and thus improve the cell healing ability of the present application, and further improve the anti-inflammatory and soothing ability of the present application.

[0063] As described above, the second aspect of the present application provides a cosmetic composition for skin, which is composed of an active ingredient and an auxiliary material, the active ingredient being the active composition of the first aspect, and the total content of the active ingredient being 5.0% by weight or more.

[0064] In the present application, the cosmetic composition containing the components in the specific amounts as described above can satisfy the effects of good anti-inflammatory, soothing, and repair.

[0065] Preferably, the content of the active ingredient is 6.0-14.0% by weight. Exemplarily, 8.0% by weight, 10.0% by weight, 12.0% by weight, 14.0% by weight.

[0066] Preferably, the auxiliary material is selected from at least one of alcohol, asiaticoside, beta-glucan, allantoin, L-rhamnose, Ultra Care RH23, and plant hydrolate; the Ultra Care RH23 is Ultra Care RH23 produced by AMT BIOTECH LTD.

[0067] Preferably, the plant hydrolate is selected from at least one of German chamomile hydrolate, lemon balm hydrolate, rose hydrolate, white orchid hydrolate, and Roman chamomile hydrolate; the alcohol is selected from at least one of glycerin, 1,3-butanediol, propylene glycol, methyl propylene glycol, panthenol, and inositol.

[0068] More preferably, the plant hydrolate is selected from at least one of rose hydrolate and white orchid hydrolate; the alcohol is selected from at least one of glycerin, panthenol, and inositol.

[0069] Preferably, the dosage form of the cosmetic composition is selected from at least one of serum, gel, water, and emulsion.

[0070] As described above, the third aspect of the present application provides the use of the cosmetic composition for skin of the second aspect in serum.

[0071] As described above, the fourth aspect of the present application provides a repair serum, which is composed of phase A, phase B, phase C, and phase D,

[0072] The phase A contains glycerin, asiaticoside, beta-glucan, rose hydrolate, white orchid hydrolate, panthenol, inositol, allantoin, and L-rhamnose.

[0073] The phase B contains glycerylphosphorylcholine salt, glucosaminoglucan, lactic acid bacteria turmeric ferment, mannose erythritol lipid, ergothioneine, seaweed oligosaccharide, hyaluronic acid, glycosylglycerin, and ekdoin.

[0074] The C phase contains Ultra Care RH23; the Ultra Care RH23 is Ultra Care RH23 produced by AMT BIOTECH LTD company;

[0075] The D phase contains 1,3-butanediol, essential oil liposome;

[0076] In the essence, the content of glycerol is 100-500 parts by weight, the content of asiaticoside is 10-70 parts by weight, the content of β-glucan is 1-30 parts by weight, the content of rose hydrolat is 1000-7600 parts by weight, the content of michelia hydrolat is 500-6500 parts by weight, the content of panthenol is 100-300 parts by weight, the content of inositol is 10-100 parts by weight, the content of allantoin is 10-25 parts by weight, and the content of L-rhamnose is 20-150 parts by weight, relative to 100 parts by weight of the glycerophosphoinositol choline salt;

[0077] The content of glucosaminoglycan is 30-70 parts by weight, the content of lactic acid bacteria turmeric ferment is 100-320 parts by weight, the content of mannose erythritol lipid is 20-50 parts by weight, the content of ergothioneine is 0.1-10 parts by weight, the content of seaweed oligosaccharide is 10-80 parts by weight, the content of hyaluronic acid is 5-50 parts by weight, the content of glycerol glucoside is 20-325 parts by weight, and the content of exopolysaccharide is 10-260 parts by weight;

[0078] The content of Ultra Care RH23 is 80-120 parts by weight;

[0079] The content of essential oil liposome is 80-500 parts by weight, and the content of 1,3-butanediol is 100-300 parts by weight.

[0080] Preferably, the content of glycerol is 150-350 parts by weight, the content of asiaticoside is 20-60 parts by weight, the content of β-glucan is 2-25 parts by weight, the content of rose hydrolat is 2500-7600 parts by weight, the content of michelia hydrolat is 500-5500 parts by weight, the content of panthenol is 120-250 parts by weight, the content of inositol is 20-80 parts by weight, the content of allantoin is 12-20 parts by weight, and the content of L-rhamnose is 25-150 parts by weight, relative to 100 parts by weight of the glycerophosphoinositol choline salt;

[0081] The content of the glucosaminoglycan is 40-60 parts by weight, the content of the Lactobacillus ferments Curcuma longa is 150-310 parts by weight, the content of the mannosylerythritol lipids is 20-40 parts by weight, the content of the ergothioneine is 2-8 parts by weight, the content of the sea algae oligosaccharides is 20-60 parts by weight, the content of the hyaluronic acid is 8-45 parts by weight, the content of the glycerol glucoside is 50-250 parts by weight, and the content of the ekotoxin is 20-220 parts by weight.

[0082] The content of the Ultra Care RH23 is 90-110 parts by weight.

[0083] The content of the essential oil liposome is 80-400 parts by weight, and the content of the 1,3-butanediol is 100-280 parts by weight. The inventors find that the serum obtained in this preferred case has more excellent anti-inflammatory, soothing and repairing effects.

[0084] Preferably, the asiaticoside is 70wt%-95wt% of the water-soluble hydroxyl asiaticoside.

[0085] The repairing serum provided by the fourth aspect of the present application can be used for anti-inflammatory, soothing and repairing of the skin.

[0086] As described above, the fifth aspect of the present application provides a method for preparing the repairing serum of the fourth aspect, which comprises:

[0087] (1) first mixing the components in phase A to obtain a mixed material I;

[0088] (2) second mixing phase B with the mixed material I to obtain a mixed material II;

[0089] (3) third mixing phase C with the mixed material II to obtain a mixed material III;

[0090] (4) fourth mixing phase D with the mixed material III to obtain the repairing serum.

[0091] Preferably, the conditions of the first mixing include a temperature of 80-85℃, a stirring speed of 30-50rpm and a time of 20-25min.

[0092] Preferably, the conditions of the second mixing include a temperature of 40-45℃, a stirring speed of 25-45rpm and a time of 25-30min.

[0093] Preferably, the conditions of the third mixing include a temperature of 25-30℃, a stirring speed of 25-45rpm and a time of 15-20min.

[0094] Preferably, the fourth mixing conditions include a temperature of 25-30°C, a stirring speed of 25-40 rpm, and a time of 25-30 min.

[0095] In the present application, the preparation methods of the A phase, the B phase, the C phase, the D phase, the mixture I, the mixture II, and the mixture III can be conventional preparation methods in the art and can be achieved by stirring.

[0096] In the present application, when the components contained in the A phase are subjected to first mixing, the components contained in the B phase are subjected to second mixing with the mixture I, the components of the C phase are subjected to third mixing with the mixture II, and the components of the D phase are subjected to fourth mixing with the mixture III, the order of addition of the components contained in the A phase, the B phase, and the C phase is not particularly limited and can be added in any order.

[0097] The present application will be described in detail below through examples.

[0098] The main raw materials in the following examples are as follows:

[0099] Glycerophosphoinositol choline salt is a commercial product GPI CHOLINE PE of CRODA SEDERMA, S.A.S;

[0100] Glucosaminoglucan is a commercial product of Shanghai Huiwen Biotechnology Co., Ltd;

[0101] Lactic acid bacteria turmeric ferment I is a commercial product Turmeric gel of Korea Nidex Pharmaceutical Co., Ltd;

[0102] Curcumin is a commercial product Curcumin C3 Complex of Sabinsa, USA;

[0103] Mannose erythritol lipid is a commercial product Bio 202 biological sugar lipid of Shanghai Yusheng Industry Co., Ltd;

[0104] Ergothioneine is a commercial product of Shenzhen Zhongkeyixian Biotechnology Co., Ltd., with a purity of 99.75%;

[0105] Alguronic acid is a commercial product of Haisheng Health Technology (Qingdao) Co., Ltd., and is developed by Qingdao Marine Biomedical Research Institute;

[0106] Alpha-glucan oligosaccharide is a commercial product of Hu Zhou Purui Biomedical Technology Co., Ltd., with a model number of alpha-glucan oligosaccharide;

[0107] Hyaluronic acid is a commercial product Supreme Hyal of Shandong Tianxun Biotechnology Co., Ltd;

[0108] Glycerol glucoside is a commercial product of Qingdao Blue Wisdom Biotech Co., Ltd. 2-αGG;

[0109] Ecdocin is a commercial product of Shandong Tian Sheng Science and Technology Co., Ltd.;

[0110] Glycerol is a commercial product of Procter & Gamble;

[0111] Madecassoside is a commercial product of Guangzhou Changzhou Natural Medicine Co., Ltd., 95wt% water-soluble hydroxyl madecassoside;

[0112] Beta-glucan is a commercial product of Sichuan Sailikang Biotechnology Co., Ltd.;

[0113] Rose hydrolate is a commercial product of Yin Gu Fragrance Technology Co., Ltd.;

[0114] Bai Lan Hua hydrolate is a commercial product of Zhengda Flower Tea Fragrance Factory;

[0115] Panthenol is a commercial product of DSM;

[0116] Myo-inositol is a commercial product of Japan Chikuno;

[0117] Allantoin is a commercial product of Germany MERK;

[0118] L-rhamnose is a commercial product of Shanghai Heping Industry Co., Ltd.;

[0119] Ultra Care RH23 is Ultra Care RH23 produced by AMT BIOTECH LTD;

[0120] 1,3-Butanediol is a commercial product of Oxea, USA;

[0121] Rome chamomile essential oil is a commercial product of Changguangzhou Fragrance Era Import and Export Co., Ltd., model Rome chamomile essential oil;

[0122] German chamomile essential oil is a commercial product of Guangzhou Fragrance Era Import and Export Co., Ltd., model German chamomile essential oil;

[0123] Italian everlasting flower essential oil is a commercial product of Guangzhou Fragrance Era Import and Export Co., Ltd., model Italian everlasting flower essential oil;

[0124] Mugwort essential oil is a commercial product of Guangzhou Fragrance Era Import and Export Co., Ltd., model Mugwort essential oil.

[0125] Preparation of essential oil liposome I:

[0126] The 20 g phospholipid, 1 g stearic acid, 1 g butanediol are uniformly stirred at 80°C until completely dissolved, then 3 g of Roman chamomile essential oil, 3 g of German chamomile essential oil, 3 g of Italian everlasting flower essential oil, 1 g of chastetree essential oil are added, and continue to stir until the mixture is uniform, to obtain an oil phase mixture. The temperature of the oil phase mixture is kept at 75°C, then 68 g of ultrapure water at 75°C is added to the oil phase mixture, and homogenized at a speed of 10000 rpm under a high-speed homogenizer for 5 min to obtain 100 g of essential oil phospholipid emulsion; then the essential oil phospholipid emulsion is injected into a microfluidic homogenizer, and sheared at a pressure of 20,000 PSI for 10 seconds, and the discharge temperature is controlled at 30°C by a heat exchanger to obtain essential oil liposome I.

[0127] Preparation of essential oil liposome II:

[0128] The preparation method of the essential oil liposome II is the same as that of the essential oil liposome I, except that "3 g of Roman chamomile essential oil, 3 g of German chamomile essential oil, 3 g of Italian everlasting flower essential oil, 1 g of chastetree essential oil" is replaced by "3 g of black spruce essential oil, 3 g of true lavender essential oil, 3 g of bitter orange flower essential oil, 1 g of turmeric essential oil", and the remaining steps are the same as those of the preparation method of the essential oil liposome I.

[0129] In the following examples, each weight part represents 1 g.

[0130] Examples 1-6

[0131] This example is used to illustrate the active composition according to the present application for repairing the essence according to the formula S1-S6 in Table 1 is added, and the essence A1-A6 is prepared according to the method as follows. The method comprises:

[0132] (1) The components in phase A are first mixed to obtain a mixture I;

[0133] The first mixing conditions are: temperature 80°C, stirring speed 40 rpm, time 25 min;

[0134] (2) The second mixing of phase B with the mixture I is carried out to obtain a mixture II;

[0135] The second mixing conditions are: temperature 45°C, stirring speed 45 rpm, time 30 min;

[0136] (3) The third mixing of phase C with the mixture II is carried out to obtain a mixture III;

[0137] The third mixing conditions are: temperature 30°C, stirring speed 35 rpm, time 20 min;

[0138] (4) mixing D phase with the mixture III to obtain the repairing serum;

[0139] The fourth mixing is carried out at 25℃, at a stirring speed of 35 rpm for 25 min.

[0140] Table 1

[0141]

[0142]

[0143] Example 7

[0144] This example is used to illustrate the preparation of the serum A7 according to the formula S1 and the method described in Example 1, except that the essential oil liposome II is used to replace the essential oil liposome I in Example 1 in equal amount.

[0145] Example 8

[0146] This example is used to illustrate the preparation of the serum A8 according to the formula S1 and the method described in Example 1, except that the curcumin is used to replace the lactic acid bacteria curcumin ferment I in Example 1 in equal amount.

[0147] Comparative Example 1

[0148] This comparative example is used to illustrate the preparation of the serum DA1 according to the formula S1 and the method described in Example 1, except that the amount of the lactic acid bacteria curcumin ferment is 350 parts by weight in this comparative example.

[0149] Comparative Example 2

[0150] This comparative example is used to illustrate the preparation of the serum DA2 according to the formula S1 and the method described in Example 1, except that the ergothioneine is not added in this comparative example.

[0151] Comparative Example 3

[0152] This comparative example is used to illustrate the preparation of the serum DA3 according to the formula S1 and the method described in Example 1, except that the α-glucan oligosaccharide is used to replace the seaweed oligosaccharide in Example 1 in equal amount.

[0153] Test Example 1: Toxicological test

[0154] The serum prepared by the method of the present application was determined according to the multiple skin irritation test method in the Cosmetic Safety Technical Specifications (2015 Edition).

[0155] The serum prepared by the example and the comparative example was tested, and each of the serum was tested on 4 healthy adult New Zealand rabbits (female, weight 2.0-2.5 kg).

[0156] The test steps were as follows: before the test, the fur on both sides of the experimental animal's back spine was cut off, and the fur removal range was about 3 cm x 3 cm on the left and right sides, respectively. 0.5 mL of the test substance was applied to the left skin as is, and the application area was 2.5 cm x 2.5 cm. When the test substance was prepared using a non-irritating solvent, the right side was applied with the solvent as a control. The application was performed once a day for 14 consecutive days. From the second day, the fur was cut off before each application, and the residue was removed with water or a non-irritating solvent. The results were observed after one hour, and the skin irritation reaction score was performed according to the relevant provisions in the Cosmetic Safety Technical Specifications (2015 Edition). After the test, the total score of 14 days, the average score of each animal per day, and the average score of each animal per day were calculated, and the skin irritation intensity classification was performed.

[0157] Observation results: the average score of each animal per day and the average score of each animal per day were 0.

[0158] The above results show that the serum prepared by the method of the present application has no skin irritation.

[0159] Test Example 2: Human patch test

[0160] The serum prepared by the example and the comparative example was tested, and each of the serum was tested on 4 healthy adult New Zealand rabbits (female, weight 2.0-2.5 kg).

[0161] Patch test method: qualified patch test equipment was selected, and the test substance about 0.020 mL-0.025 mL (liquid) was placed in the patch test equipment by the closed patch test method. The low sensitization adhesive tape was applied to the back of the test subject, and the test substance was removed after 24 hours. The skin reaction was observed at 0.5, 24, and 48 hours after removal, respectively, and the results were recorded according to the skin reaction grading standard in the current effective technical specification (Cosmetic Safety Technical Specifications (2015 Edition)).

[0162] Test results: the human skin patch test results showed that the skin reactions of the 30 test subjects were all negative reactions, indicating that the serum provided by the present application has good safety.

[0163] Test Example 3: Anti-inflammatory effect test

[0164] The serum prepared according to the examples and the comparative examples was subjected to experiments, 20 subjects were subjected to experiments for each serum, and the subjects were healthy women (i.e. non-allergic constitution) aged 20 to 53.

[0165] Usage: after cleansing, apply an appropriate amount of the serum to the face, once in the morning and once in the evening, for 28 consecutive days.

[0166] The anti-inflammatory detection method is as follows:

[0167] The skin image analysis system Visia 7 of the original imported by the American Canfield Company was used to detect and analyze all facial aging and lesion problems from the epidermis to the dermis layer by photographic means. The red area mainly analyzes the red area of the facial dermis layer, representing the location, quantity, shape, size and clinical severity of red blood vessels and inflammation. The higher the measured value, the more serious the inflammation.

[0168] The improvement rate of the red area was calculated by comparing the initial value before using the product with the value after using the product for 14 days and 28 days.

[0169] The calculation formula of the improvement rate of the red area is: {(the red area score of the subject before use-the red area score of the same subject after using for 14 days (28 days)) / the red area score of the subject before use} x 100%.

[0170] The test results are shown in Table 2:

[0171] Table 2

[0172] Before use After 14 days After 28 days Improvement rate (%) Improvement rate (%) A1 12.796 8.061 7.931 37.00 38.02 A2 17.281 9.831 8.824 43.11 48.94 A3 12.796 8.892 7.837 30.50 38.75 A4 14.124 9.072 9.226 35.77 34.68 A5 11.135 9.349 9.259 16.04 16.84 A6 11.078 9.305 9.137 16.00 17.52 A7 14.127 12.135 11.898 14.10 15.78 A8 19.412 17.601 17.077 9.34 12.03 DA1 17.974 16.597 16.308 7.66 9.27 DA2 13.875 12.812 12.718 7.67 8.34 DA3 12.179 11.672 11.563 4.16 5.06

[0173] As can be seen from the test results in Table 2, the serum provided by the present application has obvious anti-inflammatory effect.

[0174] Test Example 4: soothing effect test

[0175] The test object and test method of Test Example 4 are the same as those of Test Example 3, except that the detection method is different. The soothing effect detection method is as follows:

[0176] The facial image analyzer (manufacturer: American Canfield, model: Visia6.0) was used to analyze the cheek red area a value. The larger the measured value, the more serious the skin redness.

[0177] The improvement rate calculation formula of the a value is: {(the red area a value of the subject before use-the red area a value of the same subject after 28 days) / the red area a value of the subject before use} x 100%;

[0178] The test results are shown in Table 3:

[0179] Table 3

[0180] Before use After 28 days Improvement rate A1 29.72 18.15 38.93 A2 23.42 13.58 42.02 A3 43.02 25.39 40.98 A4 30.94 23.76 23.20 A5 38.76 31.78 18.01 A6 35.16 28.78 18.14 A7 28.54 24.26 15.00 A8 31.95 27.47 14.02 DA1 29.17 25.66 12.03 DA2 30.48 26.82 12.01 DA3 31.28 27.84 11.00

[0181] It can be seen from the test results in Table 3 that the serum provided by the present application has excellent soothing effect.

[0182] Test Example 5: Repair effect test

[0183] The test subjects and test methods of Test Example 5 are the same as those of Test Example 3, except that the detection method is different. The detection method of repair effect is as follows:

[0184] The Tewameter TM Hex probe is used to measure the data of trans-epidermal water loss rate of the cheek skin of the test subject. The smaller the measurement value, the less the amount of trans-epidermal water loss per unit time and unit cross-sectional area, and the better the skin barrier is represented.

[0185] The data of trans-epidermal water loss rate of the test subject after using the sample for 28 days and before using the sample are analyzed. The calculation formula of the improvement rate of trans-epidermal water loss rate is: {(trans-epidermal water loss rate of the skin of the test subject before use-trans-epidermal water loss rate of the same part of the same test subject after using the sample for 28 days) / trans-epidermal water loss rate of the skin of the test subject before use} x 100%.

[0186] The test results are shown in Table 4:

[0187] Table 4

[0188] Before use / g / m 2 / h]] 28 days later / g / m 2 / h]] Improvement rate / % A1 26.39 16.01 39.33 A2 21.85 11.92 45.44 A3 25.25 15.14 40.04 A4 29.43 22.44 23.75 A5 28.81 21.42 25.65 A6 25.44 19.08 25.00 A7 23.87 18.20 23.75 A8 29.86 23.05 22.81 DA1 24.81 20.09 19.02 DA2 29.87 24.57 17.74 DA3 26.12 21.67 17.04

[0189] It can be seen from the test results in Table 4 that the serum provided by the present application has good repair effect.

[0190] In addition, the present application exemplarily provides the facial images of the test subject collected by the skin detector before and after using the serum of Example 1, Example 2 and Example 4 for 14 days, which are shown in Figures 1 to 3 .

[0191] Figure 1 is a comparison chart of the facial red area of the test subject before and after using the serum of Example 2 of the present application for 14 days, Figure 1 wherein the left chart is the facial red area chart before use, and the right chart is the facial red area chart after using for 14 days.

[0192] Figure 2 is a comparison chart of the facial red area of the test subject before and after using the serum of Example 4 of the present application for 14 days, Figure 2 wherein the left chart is the facial red area chart before use, and the right chart is the facial red area chart after using for 14 days.

[0193] Figure 3is a comparison chart of the red area of the face of the subject before and after 14 days of using the serum of Example 8 of the present application, Figure 3 The left chart is a chart of the red area of the face before use, and the right chart is a chart of the red area of the face after 14 days of use.

[0194] From Figures 1 to 3 It can be seen that the serum formed by the active composition provided by the present application can significantly improve skin inflammation and improve the fullness and smoothness of the skin after 14 days of continuous use.

[0195] The above describes the preferred embodiments of the present application in detail, but the present application is not limited thereto. Within the technical concept of the present application, various simple modifications can be made to the technical solutions of the present application, including the combination of various technical features in any other suitable manner. These simple modifications and combinations should also be considered as disclosed by the present application and fall within the protection scope of the present application.

Claims

1. An active composition for anti-inflammatory, soothing, repairing of the skin, characterized in that, The active composition is composed of the following components: Glycerophosphoinositol choline salt, essential oil liposome, glucosaminoglycan, lactic acid bacteria turmeric ferment, mannose erythritol lipid, ergothioneine, seaweed oligosaccharide, hyaluronic acid, glycerol glucoside and ectoine; The content of the essential oil liposome is 100-400 parts by weight, the content of the glucosaminoglycan is 40-60 parts by weight, the content of the lactic acid bacteria turmeric ferment is 150-310 parts by weight, the content of the mannose erythritol lipid is 20-40 parts by weight, the content of the ergothioneine is 2-8 parts by weight, the content of the seaweed oligosaccharide is 20-60 parts by weight, the content of the hyaluronic acid is 8-45 parts by weight, the content of the glycerol glucoside is 50-250 parts by weight, and the content of the ectoine is 20-220 parts by weight, relative to 100 parts by weight of the glycerophosphoinositol choline salt. The essential oil liposome is prepared by emulsifying and encapsulating Roman chamomile essential oil, German chamomile essential oil, Italian everlasting flower essential oil and chastetree essential oil in phospholipids to form nanoliposomes; the essential oil liposome contains 2-5% of Roman chamomile essential oil, 2-5% of German chamomile essential oil, 1-5% of Italian everlasting flower essential oil and 0.5-2% of chastetree essential oil by weight based on the total weight of the essential oil liposome.

2. The active composition of claim 1, wherein, The lactic acid bacteria turmeric ferment is a product obtained by fermenting turmeric with a lactic acid bacteria flora; the lactic acid bacteria are at least one selected from the group consisting of Lactobacillus rhamnosus, Lactobacillus acidophilus, Lactobacillus plantarum and Lactobacillus reuteri.

3. The active composition according to claim 1 or 2, wherein, The lactic acid bacteria are Lactobacillus rhamnosus.

4. The active composition of claim 3, wherein, The seaweed oligosaccharide is a multi-component oligosaccharide mixture obtained by directional extraction from at least one marine algae selected from the group consisting of red algae, Antarctic ice algae and polar brown algae.

5. The active composition of claim 4, wherein, The seaweed oligosaccharide is Antarctic ice algae oligosaccharide.

6. The active composition of claim 5, wherein, The glycerol glucoside is 2-α configuration glycerol glucoside produced by Qingdao Zhongke Lan Zhi Biological Technology Development Co., Ltd.

7. A cosmetic composition for the skin, characterized by, The composition is composed of active ingredients and adjuvants, the active ingredients are the active composition according to any one of claims 1-6, and the total content of the active ingredients is 5.0% by weight or more.

8. The cosmetic composition according to claim 7, wherein, The adjuvants are at least one selected from the group consisting of alcohol substances, asiaticoside, β-glucan, allantoin, L-rhamnose, Ultra Care RH23 and plant hydrolat; the Ultra Care RH23 is Ultra Care RH23 produced by AMT BIOTECH LTD.

9. The cosmetic composition according to claim 8, wherein, The plant hydrolat is at least one selected from the group consisting of German chamomile hydrolat, lemon balm hydrolat, rose hydrolat, white orchid hydrolat and Roman chamomile hydrolat; the alcohol substances are at least one selected from the group consisting of glycerol, 1,3-butanediol, propylene glycol, methyl propylene glycol, panthenol and inositol.

10. The cosmetic composition according to claim 9, wherein, The dosage form of the cosmetic composition is at least one selected from the group consisting of serum, gel, water agent and emulsion.

11. Use of the cosmetic composition for skin according to any one of claims 7-10 in the preparation of a serum.

12. A repair serum, characterized in that, The serum is composed of phase A, phase B, phase C and phase D, The A phase contains glycerin, asiaticoside, beta-glucan, rose hydrolate, michelia hydrolate, panthenol, inositol, allantoin and L-rhamnose; The B phase contains glycerophosphoinositol choline salt, glucosaminoglucan, lactic acid bacteria turmeric ferment, mannose erythritol lipid, ergothioneine, algal oligosaccharide, hyaluronic acid, glycerol glucoside and ectoine; The C phase contains Ultra Care RH23; the Ultra Care RH23 is Ultra Care RH23 produced by AMT BIOTECH LTD company; The D phase contains 1,3-butanediol and essential oil liposome; In the essence, the content of glycerin is 150-350 parts by weight, the content of asiaticoside is 20-60 parts by weight, the content of beta-glucan is 2-25 parts by weight, the content of rose hydrolate is 2500-7600 parts by weight, the content of michelia hydrolate is 500-5500 parts by weight, the content of panthenol is 120-250 parts by weight, the content of inositol is 20-80 parts by weight, the content of allantoin is 12-20 parts by weight, and the content of L-rhamnose is 25-150 parts by weight, relative to 100 parts by weight of the glycerophosphoinositol choline salt; The content of glucosaminoglucan is 40-60 parts by weight, the content of lactic acid bacteria turmeric ferment is 150-310 parts by weight, the content of mannose erythritol lipid is 20-40 parts by weight, the content of ergothioneine is 2-8 parts by weight, the content of algal oligosaccharide is 20-60 parts by weight, the content of hyaluronic acid is 8-45 parts by weight, the content of glycerol glucoside is 50-250 parts by weight, and the content of ectoine is 20-220 parts by weight; The content of Ultra Care RH23 is 90-110 parts by weight; The content of essential oil liposome is 80-400 parts by weight, and the content of 1,3-butanediol is 100-280 parts by weight.

13. The repair serum of claim 12, wherein, The asiaticoside is 70wt%-95wt% of water-soluble hydroxyl asiaticoside.

14. A method of preparing the repair serum of claim 12 or 13, characterized in that, The method comprises: (1) first mixing the components in the A phase to obtain a mixed material I; (2) second mixing the B phase with the mixed material I to obtain a mixed material II; (3) third mixing the C phase with the mixed material II to obtain a mixed material III; (4) fourth mixing the D phase with the mixed material III to obtain the repair essence.

15. The method of claim 14, wherein, The conditions of the first mixing include a temperature of 80-85℃, a stirring speed of 30-50rpm and a time of 20-25min; The conditions of the second mixing include a temperature of 40-45℃, a stirring speed of 25-45rpm and a time of 25-30min; The conditions of the third mixing include a temperature of 25-30℃, a stirring speed of 25-45rpm and a time of 15-20min; The conditions of the fourth mixing include: temperature is 25-30 ℃, stirring speed is 25-40 rpm, and time is 25-30 min.

Images