High quality mixed magnetic bead bottle

Abstract

The application discloses a high-quality mixed magnetic bead bottle, which comprises a bottle body, an inwardly protruding spoiler is arranged on the inner side wall of the bottle body and extends along the length direction of the bottle body, and a transition part is arranged at the position corresponding to the two sides of the spoiler in the bottle body, wherein the cross section of the transition part is arc-shaped and protrudes towards the outside of the bottle body. The above scheme is adopted, the problem that the existing spoiler structure interferes with the flow field to cause a streamline blank area, thereby affecting the mixing uniformity and easily generating bubbles is proposed, then the structure of the transition part is introduced aiming at the problem, so as to improve the uniformity of the overall flow field in the bottle, reduce the streamline blank area, prevent the aggregation of magnetic beads, improve the mixing effect, on the other hand, reduce the pressure gradient, so as to reduce the generation of bubbles and the inclusion probability, and the purity of the magnetic bead liquid is beneficially ensured.

Description

TECHNICAL FIELD

[0001] The present application belongs to the field of diagnostic medical consumables, and particularly relates to a high-quality mixed magnetic bead bottle. BACKGROUND

[0002] The kit is one of the important carriers in the working process of the full-automatic chemical detection analyzer, and the reagent and magnetic bead liquid are usually loaded on the kit and then put into the reagent bin for rotation mixing while meeting the reagent filling requirement. The magnetic bead bottle is an important component of the kit, which is mainly used for containing magnetic bead mixed liquid, and in order to ensure the diagnosis process, the rotating magnetic bead bottle is usually used to achieve the purpose of mixing the magnetic beads.

[0003] In the prior art, in order to improve the mixing effect of the magnetic beads, a spoiler is usually arranged on the inner side of the magnetic bead bottle, such as the authorized patent with the patent number "201620699745.6" and the name "kit of electrochemical luminescence immunoassay analyzer", which discloses the related content that "the inner surface of the magnetic bead bottle 3 is axially provided with a sheet-shaped protrusion 33, which can block the rotational flow movement of the reagent in the magnetic bead bottle when the magnetic bead bottle rotates, cause turbulence, and better play a stirring role", and the authorized patent with the patent number "202221287468.X" and the name "kit and microfluidic medical analyzer", which discloses the related content that "the inner side wall of the bottle body 21 in the embodiment is provided with a turbulence part 212, and when the bottle body 21 rotates, the magnetic beads in the bottle body 21 can be effectively mixed. Exemplarily, the turbulence part 212 can be a turbulence rib or a turbulence protrusion structure".

[0004] However, the applicant found in the research process that although the above-mentioned structure is beneficial to improve the mixing effect, the flow velocity approaches to zero at the two sides of the sheet-shaped protrusion, which will cause the magnetic beads to easily gather in the area, and the filling needle is usually inserted from the upper middle position of the magnetic bead bottle to draw, and the above-mentioned type of auxiliary turbulence structure will directly cause the magnetic beads in the middle area to be extremely uneven, and the large pressure gradient of the streamline near the turbulence structure will also easily produce bubbles, which will affect the accuracy of the final diagnosis result. SUMMARY

[0005] Therefore, the present application provides a high-quality mixed magnetic bead bottle to solve the problems of uneven rotation mixing of the magnetic bead liquid in the magnetic bead bottle, easy gathering of the magnetic beads and generation of entrained bubbles in the prior art, which affect the detection result.

[0006] The technical scheme is as follows:

[0007] The utility model provides a high quality mixed magnetic bead bottle, including bottle body, the inside wall of bottle body has the turbulence portion that protrudes inwards, and the turbulence portion extends along the length direction of bottle body, its key lies in: the position of bottle body inboard corresponding turbulence portion both sides has transition part, the transition part section is arc and protrudes to the outside of bottle body.

[0008] Adopt above scheme, the transition part of arc can increase the angle of attack of turbulence portion, thereby avoid appearing low flow velocity area, improve the uniformity of whole flow field in bottle, reduce the streamline blank area near turbulence portion, under the action of drag force, magnetic bead is very easy to follow fluid flow, thereby improve mixing effect, on the other hand, reduce flow field pressure gradient, make liquid not easy to produce and mix air bubble in the mixing process, avoid liquid surface layer or inside full of air bubble after mixing, in addition compared with traditional turbulence structure, transition part can reduce liquid level fluctuation in the mixing process, under the same parameter, can improve the loading capacity of bottle.

[0009] As preferred: the transition part is symmetrically arranged on both sides of the turbulence portion. Adopt above scheme, avoid generating larger pressure gradient on both sides before and after the turbulence portion, can further improve the uniformity of flow field.

[0010] As preferred: the bottom of the inner cavity of the bottle body is conical, and the lower end of the turbulence portion extends to the middle part near the conical bottom of the inner cavity of the bottle body. Adopt above scheme, can reduce the flow field blank area in the middle part region, maximize improve the influence of the turbulence portion on the middle part.

[0011] As preferred: the chord length of the transition part gradually linearly converges in the direction from the outer edge of the conical bottom of the inner cavity of the bottle body to the middle part. Adopt above scheme, the bottom of the bottle body is conical, the space gradually reduces, and the transition part gradually converges, which can reduce the area occupied by the transition part while maintaining a larger angle of attack of the turbulence portion.

[0012] As preferred: the transition part is tangent to the turbulence portion. Adopt above scheme, can fully guarantee the smoothness of the joint part and the stability of the flow field.

[0013] As preferred: the bottle body has two turbulence portions symmetrically arranged along the axis thereof. Adopt above scheme, the symmetrically arranged turbulence portions can reduce the impact on the fluid, and try to guarantee the balance of the turbulence, thereby guaranteeing the uniformity of the overall flow field.

[0014] As preferred: the turbulence portions are even in number and uniformly distributed in the circumferential direction of the bottle body. Adopt above scheme, the uniformly distributed multiple turbulence portions can further improve the turbulence effect without affecting the stability and uniformity of the flow field.

[0015] As preferred: the thickness of the spoiler protruding into the bottle body is d, the sum of the tangent angles of the transition part and the spoiler is a, the inner diameter of the bottle body is [10mm, 24mm], and the range of the a is (0°, 150°]. With the above scheme, within the range of the existing general bottle body size, the range of the a is preferably ensured, so that the transition part can improve the flow field on both sides of the spoiler and reduce the streamline blank area.

[0016] As preferred: the loading height of the bottle body 1 is [10mm, 55mm], and the working speed of the bottle body (1) is V;

[0017] When the d is (0mm, 2mm], the V is (1000rpm, 1400rpm], and the range of the a is [100°, 150°];

[0018] When the d is (0mm, 4mm], the V is (500rpm, 1000rpm], and the range of the a is [60°, 150°];

[0019] When the d is [4mm,], the V is (0rpm, 500rpm), and the range of the a is (0°, 60°].

[0020] With the above scheme, according to the loading amount and the working speed of the bottle body during use, the thickness d of the spoiler protrusion is further preferred, and the sum of the tangent angles a is further preferred. Different ranges of the a meet different situations, so that the flow field blank area is controlled within the minimum range.

[0021] As preferred: the outer wall of the bottle body 1 is recessed inward to form a groove 4 corresponding to the positions of the spoiler 2 and the transition part 3. With the above scheme, the mold is integrally formed, which reduces the processing difficulty and production cost.

[0022] Compared with the prior art, the beneficial effects of the present application are:

[0023] The high-quality mixed magnetic bead bottle provided by the present application pays attention to and points out that the existing spoiler structure interferes with the flow field to cause a streamline blank area, which further affects the mixing uniformity and easily produces bubbles. Then, the structure of the transition part is introduced to improve the uniformity of the overall flow field in the bottle, reduce the streamline blank area, prevent the aggregation of magnetic beads, improve the mixing effect, reduce the pressure gradient on the other hand, thereby reducing the probability of bubble generation and inclusion, and being conducive to ensuring the purity of the magnetic bead liquid. BRIEF DESCRIPTION OF DRAWINGS

[0024] Figure 1 It is a structure perspective view of the present application;

[0025] Figure 2 It is a structure perspective view of the present application;Figure 1 Internal structure diagram;

[0026] Figure 3 For Figure 1 Top view;

[0027] Figure 4 For Figure 1 Side view;

[0028] Figure 5 For Figure 4 Sectional view at A-A;

[0029] Figure 6 For the invention is a schematic diagram of the spoiler and transition;

[0030] Figure 7 For the traditional bottle fluid flow state simulation diagram;

[0031] Figure 8 For the application bottle fluid flow state simulation diagram;

[0032] Figure 9 For the fitting relationship between a and r. DETAILED DESCRIPTION

[0033] The application will be further described in detail below with reference to the accompanying drawings.

[0034] Referring to Figures 1 to 9 The high-quality mixed magnetic beads bottle shown, including a generally hollow cylindrical structure of the bottle 1, usually bottle 1 is configured with a threaded screw cap (not shown), in order to prevent when the bottle 1 high speed rotation, the internal magnetic beads relative to stationary or idle speed so as to affect the mixing, therefore in the bottle 1 of the inner side wall is usually provided with spoiler 2, spoiler 2 to the bottle 1 of the inner side protruding, and usually along the length direction of the bottle 1 (when using the height direction) extends.

[0035] First, combined with Figure 7 It can be seen that Figure 7 a for the flow field under the simulation of the schematic, Figure 7 b for the flow field under the simulation of the schematic), the traditional bottle because of the introduction of spoiler, and has a large angle of attack, as shown, fluid is blocked by spoiler, flow lines are blocked, after the rib will produce a large piece of no liquid flow line of blank area, the liquid flow rate of this area is very low, in Figure 7The speed field is embodied as an outer blue area, and magnetic beads are easily gathered in the area and are not easily mixed. On the other hand, according to Bernoulli's principle, the faster the fluid flow, the smaller the pressure of the fluid flow, so the liquid in the flow line blank area flows to the area with high flow rate under the pressure gradient, causing the liquid to be absent at the inner wall, and the air in the atmosphere is used to fill the vacancy, so that during the rotation mixing process, air is mixed into the liquid, and when the mixing stops, the air bubbles float up, and the unbroken air-liquid surface bubbles are formed, and the small air bubbles are likely to adhere to the inner wall of the magnetic bead bottle. The larger the flow line blank area, the greater the flow rate difference of the liquid, the greater the pressure gradient, and the greater the probability of air bubbles generated during the mixing process, which will also affect the purity and uniformity of the final magnetic bead mixed liquid.

[0036] The present application is aware of this problem, and therefore a transition part 3 is arranged in the bottle body 1 corresponding to the position of the spoiler part 2. As shown in the figure, the cross section of the transition part 3 is basically arc-shaped and protrudes towards the outside of the bottle body 1, mainly used to improve the angle of attack of the spoiler part 2.

[0037] In specific implementation, the transition part 3 is symmetrically arranged on both sides of the spoiler part 2, that is, the angles of attack on both sides of the spoiler part 2 are improved, so that no matter the bottle body 1 is rotating or counter-rotating, the flow field and flow line can be improved through the transition part 3.

[0038] In the embodiment, the bottom of the inner cavity of the bottle body 1 is conical, the lower end of the spoiler part 2 extends to the middle part near the conical bottom of the inner cavity of the bottle body 1, and the protruding thickness of the spoiler part 2 also gradually decreases to zero. Along the direction from the outer edge of the conical bottom of the inner cavity of the bottle body 1 to the middle part, the chord length of the transition part 3 gradually converges linearly, so that Figure 3 For reference, the width of the transition part 3 gradually decreases.

[0039] Generally, there are an even number of spoiler parts 2 in the bottle body 1, and all the spoiler parts 2 are uniformly distributed along the circumferential direction of the bottle body 1. In the embodiment, the bottle body 1 has two spoiler parts 2 arranged symmetrically along the axis thereof.

[0040] For reference Figure 6 To fully utilize the spoiler effect of the spoiler part 2, in specific implementation, the spoiler part 2 is a regular circular arc, and the vertex of the circular arc is directly opposite the axis of the bottle body 1, that is, the corresponding diameter of the spoiler part 2 and the corresponding diameter of the bottle body 1 must coincide. At the same time, the transition part 3 is tangent to the spoiler part 2, that is, the transition part 3 is also a regular circular arc, and its corresponding circle is tangent to the corresponding circle of the spoiler part 2, so as to further ensure the smoothness of the angle of attack.

[0041] As shown in the figure, the thickness of the spoiler part 2 protruding into the bottle body 1 is d, the sum of the tangent angles of the two transition parts 3 and the spoiler part 2 is α, the inner diameter of the bottle body 1 is [10mm, 24mm], and the range of α is (0°, 150°].

[0042] The loading height of bottle 1 is [10mm, 55mm], the operating speed of bottle 1 during loading and mixing is V, and the radius of the circle corresponding to the turbulence part 2 is r (in mm). During the rotation and mixing process, d and V are the two main parameters affecting the flow state. However, based on the current size limitations of bottle 1 and the limitations of the mixing drive structure, the two parameters d and V are limited to a certain range. In this application, under the premise of the above loading height and the diameter of bottle 1, α and r are further optimized according to the limited range of d and V.

[0043] When d is in (0mm, 2mm] and V is in (1000rpm, 1400rpm], the range of α is [100°, 150°].

[0044] When d is in (0mm, 4mm] and V is in (500rpm, 1000rpm], the range of α is [60°, 150°].

[0045] When d is in [4mm,), i.e. d≥4mm, and V is in (0rpm,500rpm), the range of α is (0°,60°).

[0046] And fit the relationship between α and r as follows: Figure 9 As shown, the relation is obtained:

[0047]

[0048] in For the fitted values, the physical units of the parameters are ignored in this relation, and only numerical fitting is performed. Based on the values ​​of d, V, and α, and the above fitting relation, the range of r is further optimized as follows:

[0049]

[0050] Furthermore, in this application, the turbulence-disrupting part 2 and the transition part 3 are integrally formed with the bottle body 1, and the outer wall of the bottle body 1 is recessed inward to form a groove 4 corresponding to the positions of the turbulence-disrupting part 2 and the transition part 3. In addition, the height of the turbulence-disrupting part 2 is usually lower than the position of the upper screw thread of the bottle body 1 to prevent the turbulence from causing the fluid to splash out during rotation.

[0051] Of course, because the transition section 3 in this application greatly reduces the fluctuation range of the liquid during the mixing process, it can hold more liquid compared with the traditional magnetic bead bottle with the same parameters, thereby improving the utilization efficiency of the magnetic bead bottle, extending the duration of the magnetic bead bottle, reducing the frequency of replacement, and helping to ensure the constant temperature environment of the use environment (reagent chamber).

[0052] Combination Figure 8 It can be known that ( Figure 8 a is a schematic diagram of the flow velocity field under the simulation of this application. Figure 8b is the streamline field change under the simulation of the application, after the transition part 3 improves the angle of attack of the spoiler part 2, because the low flow area is avoided by the large round transition, the fluid streamline in this area is also relatively smooth, under the action of drag force, the magnetic beads are very easy to follow the liquid flow, the magnetic beads are not easy to gather outside the corner, and the mixing is easier to achieve, the mixing effect is improved, in addition, the flow speed vacancy is also obviously reduced (as shown by the outer blue part), the streamline is filled more, the pressure gradient is reduced, which is beneficial to reduce the generation of bubbles and the probability of inclusion, ensure that there are few bubbles in the mixed liquid, which is beneficial to ensure the purity of the liquid and will not affect the normal drawing of the filling needle.

[0053] Finally, it should be noted that the above description is only for the preferred embodiments of the present application, and those skilled in the art can make various similar expressions under the inspiration of the present application without departing from the purpose of the present application and the claims, and such changes fall within the protection scope of the present application.

Claims

1. A high-quality mixed magnetic bead bottle, comprising a bottle body (1), the inner side wall of the bottle body (1) has an inwardly protruding spoiler (2), and the spoiler (2) extends along the length direction of the bottle body (1), characterized in that: The bottle body (1) has transition sections (3) corresponding to the positions on both sides of the spoiler section (2) in the bottle body (1), the transition sections (3) are arc-shaped in cross section and protrude towards the outside of the bottle body (1); The bottom of the inner cavity of the bottle body (1) is conical, the lower end of the spoiler section (2) extends to the middle of the conical bottom of the inner cavity of the bottle body (1), and the chord length of the transition section (3) gradually linearly converges along the direction from the outer edge to the middle of the conical bottom of the inner cavity of the bottle body (1); The transition sections (3) are tangent to the spoiler section (2); The thickness of the spoiler section (2) protruding into the bottle body (1) is d, the sum of the tangent angles of the transition section (3) and the spoiler section (2) is α, the inner diameter of the bottle body (1) is [10mm, 24mm], wherein the range of α is (0°, 150°], and the corresponding radius of the spoiler section (2) is r, then wherein is the fitted value, The loading height of the bottle body (1) is [10mm, 55mm], and the working speed of the bottle body (1) is V; When the d is in (0mm, 2mm], V is in (1000rpm, 1400rpm], the range of α is [100°, 150°]; When the d is in (0mm, 4mm], V is in (500rpm, 1000rpm], the range of α is [60°, 150°]; When the d is in [4mm, ), V is in (0rpm, 500rpm), the range of α is (0°, 60°].

2. The high-quality mixed magnetic bead bottle of claim 1, wherein: The transition sections (3) are symmetrically arranged on both sides of the spoiler section (2).

3. The high-quality mixed magnetic bead bottle according to claim 1 or 2, characterized in that: The bottle body (1) has two symmetrically arranged spoiler sections (2) along the axis thereof.

4. The high-quality mixed magnetic bead bottle according to claim 1 or 2, characterized in that: The spoiler section (2) has an even number and is uniformly distributed along the circumferential direction of the bottle body (1).

5. The high-quality mixed magnetic bead bottle according to claim 1 or 2, characterized in that: The outer wall of the bottle body (1) is recessed inward to form a groove (4) corresponding to the positions of the spoiler section (2) and the transition section (3).

Images